Pcsk6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcsk6
Entrez Gene ID	25507
Full Name	proprotein convertase subtilisin/kexin type 6
Synonyms	Pace4,Spc4
General protein information	Preferred Names proprotein convertase subtilisin/kexin type 6 Names proprotein convertase subtilisin/kexin type 6 Subtilisin - like endoprotease kexin-like protease PC7A paired basic amino acid cleaving enzyme 4 subtilisin-like proprotein convertase 4 subtilisin/kexin-like protease PACE4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	may play a role in neuroendocrine peptide processing [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012999.1	NP_037131.1	proprotein convertase subtilisin/kexin type 6 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PCSK6	NP_002561.1
Canis lupus familiaris (dog)	PCSK6	XP_005618782.1
Bos taurus (cattle)	PCSK6	XP_002696700.3
Mus musculus (house mouse)	Pcsk6	NP_035178.1
Rattus norvegicus (Norway rat)	Pcsk6	NP_037131.1
Pan troglodytes (chimpanzee)	PCSK6	XP_001146976.3
Gallus gallus (chicken)	PCSK6	XP_413892.4
	LOC100429418	XP_002805017.1
Xenopus tropicalis (tropical clawed frog)	pcsk6	XP_002939308.2

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of pro-protein convertase subtilisin/kexin type 6 has a protective role against synovitis in a rat model of rheumatoid arthritis.
Jiang H, Wang L, Pan J
Molecular medicine reports12(5)7681-6(2015 Nov)

Furin is the primary in vivo convertase of angiopoietin-like 3 and endothelial lipase in hepatocytes.
Essalmani R, Susan-Resiga D, Chamberland A, Asselin MC, Canuel M, Constam D, Creemers JW, Day R, Gauthier D, Prat A, Seidah NG
The Journal of biological chemistry288(37)26410-8(2013 Sep)

Inhibition and transcriptional silencing of a subtilisin-like proprotein convertase, PACE4/SPC4, reduces the branching morphogenesis of and AQP5 expression in rat embryonic submandibular gland.
Akamatsu T, Azlina A, Purwanti N, Karabasil MR, Hasegawa T, Yao C, Hosoi K
Developmental biology325(2)434-43(2009 Jan)

Temporospatially regulated expression of subtilisin-like proprotein convertase PACE4 (SPC4) during development of the rat submandibular gland.
Akamatsu T, Purwanti N, Karabasil MR, Li X, Yao C, Kanamori N, Hosoi K
Developmental dynamics : an official publication of the American Association of Anatomists236(1)314-20(2007 Jan)

A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4.
Tsuji A, Kikuchi Y, Sato Y, Koide S, Yuasa K, Nagahama M, Matsuda Y
The Biochemical journal396(1)51-9(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PCSK6 knockdown significantly decreased the cell proliferation, invasion and migration of fibroblastlike synoviocytes from rats with collageninduced arthritis.
Title: Inhibition of pro-protein convertase subtilisin/kexin type 6 has a protective role against synovitis in a rat model of rheumatoid arthritis.

PACE4 regulates the SMG development via the activation of some growth/differentiation factors.
Title: Inhibition and transcriptional silencing of a subtilisin-like proprotein convertase, PACE4/SPC4, reduces the branching morphogenesis of and AQP5 expression in rat embryonic submandibular gland.

temporospatially regulated expression of PACE4 suggests its apparent association with the proliferation, differentiation, and establishment of functional acinar and ductal cells of the submandibular gland.
Title: Temporospatially regulated expression of subtilisin-like proprotein convertase PACE4 (SPC4) during development of the rat submandibular gland.

Selective and transient association of RCN-3 with the precursor of PACE4 plays an important role in the biosynthesis of PACE4.
Title: A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4.

The following Pcsk6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcsk6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01601	NM_012999.1 Latest version!	Rattus norvegicus proprotein convertase subtilisin/kexin type 6 (Pcsk6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$552.30 $789.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcsk6 ( NM_012999.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037131.1 Rattus norvegicus proprotein convertase subtilisin/kexin type 6 (Pcsk6), mRNA.

Price & Availability↑

CloneID	ORa01601
Clone ID Related Accession (Same CDS sequence)	NM_012999.1
Accession Version	NM_012999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2814bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Rattus norvegicus(Norway rat)
Product	proprotein convertase subtilisin/kexin type 6 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L31894.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L31894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCTCCGC GCGCGCCGCC AGCGCCCGGG CCCCGGCCGC CGCCCCGGGC CGCCGGCCGG 
CACGGGCTCT CGCCGCTGGC GCCGCGCCCC TGGCGTTGGC TGCTTCTGCT CGCTCTGCCC 
GCCGTCTGCT CCGCGCTGCC GCCGCCGCGC CCCGTCTACA CCAACCACTG GGCAGTGCAA 
GTGCTGGGCG GCCCCGGCGC GGCGGACCGC GTGGCTGCGG CTCACGGCTA CCTCAACTTG 
GGCCAGATTG GAAATCTGGA CGATTACTAT CATTTTTACC ACAGCAAGAC CTTCAAGAGA 
TCAACCTTGA GTAGCAGGGG CCCCCACACC TTCCTCAGAA TGGACCCACA GGTAAAATGG 
CTCCAGCAAC AGGAAGTGAA ACGCAGGGTC AAGAGACAGG CGCGAAGCGA CTCTCTTTAT 
TTCAATGATC CCATTTGGTC CAACATGTGG TATATGCATT GTGCTGATAA GAACAGTCGC 
TGTCGGTCAG AGATGAACGT CCAGGCGGCA TGGAAGCGCG GCTACACAGG AAAGAACGTG 
GTTGTCACCA TCCTCGATGA CGGCATAGAA AGGAATCACC CAGACCTGGC CCCCAACTAC 
GATTCCTATG CAAGCTACGA TGTCAACGGA AACGATTATG ACCCATCACC GAGATATGAT 
GCCAGCAACG AGAACAAACA TGGTACTCGC TGTGCGGGAG AAGTCGCTGC TTCAGCCAAC 
AACTCCTACT GCATCGTGGG CATAGCATAT AATGCAAAGA TAGGAGGCAT CCGGATGCTG 
GACGGTGACG TGACCGACGT GGTTGAGGCC AAGTCTCTGG GCATCAGACC CAACTACATT 
GACATTTACA GCGCTAGTTG GGGGCCAGAT GATGATGGGA AGACCGTGGA TGGGCCCGGC 
CGTCTGGCTA AACAGGCTTT CGAGTATGGC ATTAAAAAGG GCCGCCAAGG TCTGGGCTCC 
ATTTTTGTCT GGGCCTCTGG GAATGGTGGG AGAGAAGGGG ACCACTGCTC CTGTGATGGC 
TACACCAACA GCATCTACAC CATCTCTGTG AGCAGCACCA CTGAGAACGG CCACAAACCC 
TGGTACCTGG AGGAATGTGC TTCCACCTTG GCTACCACCT ACAGCAGCGG GGCCTTCTAT 
GAACGGAAGA TCGTCACCAC GGACCTGCGT CAGCGCTGCA CCGACGGCCA CACTGGGACA 
TCTGTCTCAG CTCCCATGGT GGCTGGCATC ATTGCCCTGG CTCTAGAAGC AAACAACCAG 
TTGACCTGGA GGGACGTGCA GCACCTGTTA GTAAAGACGT CACGGCCGGC TCATCTGAAG 
GCGAGTGACT GGAAAGTCAA CGGAGCTGGG CATAAAGTTA GCCATCTCTA TGGATTTGGC 
TTGGTGGATG CTGAAGCGCT CGTCCTAGAG GCAAGGAAGT GGACGGCAGT GCCATCCCAG 
CACATGTGCG TGGCCACCGC AGACAAAAGG CCCAGGAGCA TCCCCGTAGT GCAGGTGCTG 
CGGACCACAG CCCTGACCAA TGCCTGTGCA GACCACTCTG ACCAGCGTGT GGTGTACCTG 
GAGCATGTGG TAGTCCGAAT CTCTATCTCA CATCCACGAC GGGGTGACCT CCAGATCCAC 
CTGATTTCTC CCTCTGGAAC CAAGTCTCAA CTTTTGGCAA AGAGATTGCT GGATTTTTCC 
AATGAGGGGT TCACGAACTG GGAGTTCATG ACTGTCCACT GCTGGGGAGA AAAGGCTGAA 
GGTGAATGGA CCCTGGAAGT CCAGGATATA CCATCGCAGG TCCGCAACCC AGAGAAACAA 
GGAAAGTTGA AAGAATGGAG CCTCATTTTA TATGGCACTG CAGAGCACCC ATACCGCACC 
TTCAGCTCCC ACCAGTCTCG CTCACGGATG CTGGAGCTTT CAGTCCCGGA ACAGGAGCCT 
CTCAAGGCTG AGGGACCACC ACCGCAGGCA GAGACTCCAG AAGAAGAGGA AGAGTACACA 
GGTGTGTGCC ATCCAGAGTG TGGTGATAAA GGCTGCGATG GTCCCAGTGC AGACCAGTGC 
TTGAACTGCG TCCACTTCAG CCTGGGAAAC TCCAAGACAA ACAGGAAGTG TGTGAGCGAG 
TGCCCCTTGG GCTACTTTGG GGACACAGCA GCAAGACGCT GCCGTCGATG CCATAAGGGA 
TGTGAGACAT GCACGGGCAG GAGCCCAACA CAGTGCCTGT CTTGTCGCCG TGGGTTCTAT 
CACCACCAGG AAACGAACAC ATGTGTGACC CTGTGTCCTG CCGGACTTTA TGCTGATGAA 
AGTCAGAGAC TCTGCCTCAG GTGCCACCCG AGCTGTCAGA AGTGTGTGGA TGAACCTGAG 
AAGTCGACTG TGTGCAAGGA GGGATTCAGC CTCGCACGGG GCAGCTGCAT TCCGGACTGT 
GAACCAGGTA CCTACTTCGA TTCTGAGCTC ATCAGATGTG GGGAATGCCA TCACACCTGC 
CGGACCTGCG TGGGGCCCAG CAGAGAAGAA TGTATTCACT GTGCAAAAAG CTTCCACTTC 
CAAGACTGGA AATGTGTGCC GGCCTGTGGT GAGGGCTTCT ACCCGGAGGA GATGCCTGGC 
TTACCCCACA AAGTGTGTCG AAGATGTGAT GAAAACTGCC TGAGCTGCGA GGGCTCCAGC 
AGGAACTGCA GCAGATGTAA AGCTGGCTTC ACGCAGCTGG GGACCTCCTG CATCACCAAC 
CACACGTGCA GTAACGCCGA TGAGACCTTC TGCGAGATGG TAAAGTCCAA CCGGCTCTGT 
GAACGGAAGC TCTTCATCCA GTTTTGTTGC CGCACCTGCC TCCTGGCTGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037131.1
CDS	29..2842
Translation	MPPRAPPAPGPRPPPRAAGRHGLSPLAPRPWRWLLLLALPAVCSALPPPRPVYTNHWAVQVLGGPGAADRVAAAHGYLNLGQIGNLDDYYHFYHSKTFKRSTLSSRGPHTFLRMDPQVKWLQQQEVKRRVKRQARSDSLYFNDPIWSNMWYMHCADKNSRCRSEMNVQAAWKRGYTGKNVVVTILDDGIERNHPDLAPNYDSYASYDVNGNDYDPSPRYDASNENKHGTRCAGEVAASANNSYCIVGIAYNAKIGGIRMLDGDVTDVVEAKSLGIRPNYIDIYSASWGPDDDGKTVDGPGRLAKQAFEYGIKKGRQGLGSIFVWASGNGGREGDHCSCDGYTNSIYTISVSSTTENGHKPWYLEECASTLATTYSSGAFYERKIVTTDLRQRCTDGHTGTSVSAPMVAGIIALALEANNQLTWRDVQHLLVKTSRPAHLKASDWKVNGAGHKVSHLYGFGLVDAEALVLEARKWTAVPSQHMCVATADKRPRSIPVVQVLRTTALTNACADHSDQRVVYLEHVVVRISISHPRRGDLQIHLISPSGTKSQLLAKRLLDFSNEGFTNWEFMTVHCWGEKAEGEWTLEVQDIPSQVRNPEKQGKLKEWSLILYGTAEHPYRTFSSHQSRSRMLELSVPEQEPLKAEGPPPQAETPEEEEEYTGVCHPECGDKGCDGPSADQCLNCVHFSLGNSKTNRKCVSECPLGYFGDTAARRCRRCHKGCETCTGRSPTQCLSCRRGFYHHQETNTCVTLCPAGLYADESQRLCLRCHPSCQKCVDEPEKSTVCKEGFSLARGSCIPDCEPGTYFDSELIRCGECHHTCRTCVGPSREECIHCAKSFHFQDWKCVPACGEGFYPEEMPGLPHKVCRRCDENCLSCEGSSRNCSRCKAGFTQLGTSCITNHTCSNADETFCEMVKSNRLCERKLFIQFCCRTCLLAG

Target ORF information:

RefSeq Version	NM_012999.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proprotein convertase subtilisin/kexin type 6 (Pcsk6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012999.1

ORF Insert Sequence:

ATGCCTCCGC GCGCGCCGCC AGCGCCCGGG CCCCGGCCGC CGCCCCGGGC CGCCGGCCGG 
CACGGGCTCT CGCCGCTGGC GCCGCGCCCC TGGCGTTGGC TGCTTCTGCT CGCTCTGCCC 
GCCGTCTGCT CCGCGCTGCC GCCGCCGCGC CCCGTCTACA CCAACCACTG GGCAGTGCAA 
GTGCTGGGCG GCCCCGGCGC GGCGGACCGC GTGGCTGCGG CTCACGGCTA CCTCAACTTG 
GGCCAGATTG GAAATCTGGA CGATTACTAT CATTTTTACC ACAGCAAGAC CTTCAAGAGA 
TCAACCTTGA GTAGCAGGGG CCCCCACACC TTCCTCAGAA TGGACCCACA GGTAAAATGG 
CTCCAGCAAC AGGAAGTGAA ACGCAGGGTC AAGAGACAGG CGCGAAGCGA CTCTCTTTAT 
TTCAATGATC CCATTTGGTC CAACATGTGG TATATGCATT GTGCTGATAA GAACAGTCGC 
TGTCGGTCAG AGATGAACGT CCAGGCGGCA TGGAAGCGCG GCTACACAGG AAAGAACGTG 
GTTGTCACCA TCCTCGATGA CGGCATAGAA AGGAATCACC CAGACCTGGC CCCCAACTAC 
GATTCCTATG CAAGCTACGA TGTCAACGGA AACGATTATG ACCCATCACC GAGATATGAT 
GCCAGCAACG AGAACAAACA TGGTACTCGC TGTGCGGGAG AAGTCGCTGC TTCAGCCAAC 
AACTCCTACT GCATCGTGGG CATAGCATAT AATGCAAAGA TAGGAGGCAT CCGGATGCTG 
GACGGTGACG TGACCGACGT GGTTGAGGCC AAGTCTCTGG GCATCAGACC CAACTACATT 
GACATTTACA GCGCTAGTTG GGGGCCAGAT GATGATGGGA AGACCGTGGA TGGGCCCGGC 
CGTCTGGCTA AACAGGCTTT CGAGTATGGC ATTAAAAAGG GCCGCCAAGG TCTGGGCTCC 
ATTTTTGTCT GGGCCTCTGG GAATGGTGGG AGAGAAGGGG ACCACTGCTC CTGTGATGGC 
TACACCAACA GCATCTACAC CATCTCTGTG AGCAGCACCA CTGAGAACGG CCACAAACCC 
TGGTACCTGG AGGAATGTGC TTCCACCTTG GCTACCACCT ACAGCAGCGG GGCCTTCTAT 
GAACGGAAGA TCGTCACCAC GGACCTGCGT CAGCGCTGCA CCGACGGCCA CACTGGGACA 
TCTGTCTCAG CTCCCATGGT GGCTGGCATC ATTGCCCTGG CTCTAGAAGC AAACAACCAG 
TTGACCTGGA GGGACGTGCA GCACCTGTTA GTAAAGACGT CACGGCCGGC TCATCTGAAG 
GCGAGTGACT GGAAAGTCAA CGGAGCTGGG CATAAAGTTA GCCATCTCTA TGGATTTGGC 
TTGGTGGATG CTGAAGCGCT CGTCCTAGAG GCAAGGAAGT GGACGGCAGT GCCATCCCAG 
CACATGTGCG TGGCCACCGC AGACAAAAGG CCCAGGAGCA TCCCCGTAGT GCAGGTGCTG 
CGGACCACAG CCCTGACCAA TGCCTGTGCA GACCACTCTG ACCAGCGTGT GGTGTACCTG 
GAGCATGTGG TAGTCCGAAT CTCTATCTCA CATCCACGAC GGGGTGACCT CCAGATCCAC 
CTGATTTCTC CCTCTGGAAC CAAGTCTCAA CTTTTGGCAA AGAGATTGCT GGATTTTTCC 
AATGAGGGGT TCACGAACTG GGAGTTCATG ACTGTCCACT GCTGGGGAGA AAAGGCTGAA 
GGTGAATGGA CCCTGGAAGT CCAGGATATA CCATCGCAGG TCCGCAACCC AGAGAAACAA 
GGAAAGTTGA AAGAATGGAG CCTCATTTTA TATGGCACTG CAGAGCACCC ATACCGCACC 
TTCAGCTCCC ACCAGTCTCG CTCACGGATG CTGGAGCTTT CAGTCCCGGA ACAGGAGCCT 
CTCAAGGCTG AGGGACCACC ACCGCAGGCA GAGACTCCAG AAGAAGAGGA AGAGTACACA 
GGTGTGTGCC ATCCAGAGTG TGGTGATAAA GGCTGCGATG GTCCCAGTGC AGACCAGTGC 
TTGAACTGCG TCCACTTCAG CCTGGGAAAC TCCAAGACAA ACAGGAAGTG TGTGAGCGAG 
TGCCCCTTGG GCTACTTTGG GGACACAGCA GCAAGACGCT GCCGTCGATG CCATAAGGGA 
TGTGAGACAT GCACGGGCAG GAGCCCAACA CAGTGCCTGT CTTGTCGCCG TGGGTTCTAT 
CACCACCAGG AAACGAACAC ATGTGTGACC CTGTGTCCTG CCGGACTTTA TGCTGATGAA 
AGTCAGAGAC TCTGCCTCAG GTGCCACCCG AGCTGTCAGA AGTGTGTGGA TGAACCTGAG 
AAGTCGACTG TGTGCAAGGA GGGATTCAGC CTCGCACGGG GCAGCTGCAT TCCGGACTGT 
GAACCAGGTA CCTACTTCGA TTCTGAGCTC ATCAGATGTG GGGAATGCCA TCACACCTGC 
CGGACCTGCG TGGGGCCCAG CAGAGAAGAA TGTATTCACT GTGCAAAAAG CTTCCACTTC 
CAAGACTGGA AATGTGTGCC GGCCTGTGGT GAGGGCTTCT ACCCGGAGGA GATGCCTGGC 
TTACCCCACA AAGTGTGTCG AAGATGTGAT GAAAACTGCC TGAGCTGCGA GGGCTCCAGC 
AGGAACTGCA GCAGATGTAA AGCTGGCTTC ACGCAGCTGG GGACCTCCTG CATCACCAAC 
CACACGTGCA GTAACGCCGA TGAGACCTTC TGCGAGATGG TAAAGTCCAA CCGGCTCTGT 
GAACGGAAGC TCTTCATCCA GTTTTGTTGC CGCACCTGCC TCCTGGCTGG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of pro-protein convertase subtilisin/kexin type 6 has a protective role against synovitis in a rat model of rheumatoid arthritis.
Molecular medicine reports12(5)7681-6(2015 Nov)
Jiang H,Wang L,Pan J

Furin is the primary in vivo convertase of angiopoietin-like 3 and endothelial lipase in hepatocytes.
The Journal of biological chemistry288(37)26410-8(2013 Sep)
Essalmani R,Susan-Resiga D,Chamberland A,Asselin MC,Canuel M,Constam D,Creemers JW,Day R,Gauthier D,Prat A,Seidah NG

Inhibition and transcriptional silencing of a subtilisin-like proprotein convertase, PACE4/SPC4, reduces the branching morphogenesis of and AQP5 expression in rat embryonic submandibular gland.
Developmental biology325(2)434-43(2009 Jan)
Akamatsu T,Azlina A,Purwanti N,Karabasil MR,Hasegawa T,Yao C,Hosoi K

Temporospatially regulated expression of subtilisin-like proprotein convertase PACE4 (SPC4) during development of the rat submandibular gland.
Developmental dynamics : an official publication of the American Association of Anatomists236(1)314-20(2007 Jan)
Akamatsu T,Purwanti N,Karabasil MR,Li X,Yao C,Kanamori N,Hosoi K

A proteomic approach reveals transient association of reticulocalbin-3, a novel member of the CREC family, with the precursor of subtilisin-like proprotein convertase, PACE4.
The Biochemical journal396(1)51-9(2006 May)
Tsuji A,Kikuchi Y,Sato Y,Koide S,Yuasa K,Nagahama M,Matsuda Y

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Pcsk6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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