Nedd4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nedd4
Entrez Gene ID	25489
Full Name	neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase
Synonyms	Nedd4a
General protein information	Preferred Names neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase NEDD4 HECT-type E3 ubiquitin transferase NEDD4 neural precursor cell expressed, developmentally down-regulated gene 4 neural precursor cell expressed, developmentally down-regulated gene 4A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	may regulate function of the amiloride-sensitive epithelial sodium channel ENaC [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012986.1	NP_037118.1	E3 ubiquitin-protein ligase NEDD4
XM_017595479.1	XP_017450968.1	E3 ubiquitin-protein ligase NEDD4 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nedd4a	NP_001029358.1
Mus musculus (house mouse)	Nedd4	NP_035020.2
Schizosaccharomyces pombe (fission yeast)	pub3	NP_595793.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003133	XP_003436401.1
Pan troglodytes (chimpanzee)	NEDD4	XP_523083.3
Rattus norvegicus (Norway rat)	Nedd4	NP_037118.1

Gene Ontology
Bibliography

Related articles in PubMed

NEDD4-1 protects against ischaemia/reperfusion-induced cardiomyocyte apoptosis via the PI3K/Akt pathway.
Hu W, Zhang P, Gu J, Yu Q, Zhang D
Apoptosis : an international journal on programmed cell death22(3)437-448(2017 Mar)

Proanthocyanidins block aldosterone-dependent up-regulation of cardiac gamma ENaC and Nedd4-2 inactivation via SGK1.
Galiana-Simal A, Olivares-?lvaro E, Klett-Mingo M, Ruiz-Roso MB, Ballesteros S, de Las Heras N, Fuller PJ, Lahera V, Mart?n-Fern?ndez B
The Journal of nutritional biochemistry3713-19(2016 11)

Structural Studies of the Nedd4 WW Domains and Their Selectivity for the Connexin43 (Cx43) Carboxyl Terminus.
Spagnol G, Kieken F, Kopanic JL, Li H, Zach S, Stauch KL, Grosely R, Sorgen PL
The Journal of biological chemistry291(14)7637-50(2016 Apr)

Histone Modification of Nedd4 Ubiquitin Ligase Controls the Loss of AMPA Receptors and Cognitive Impairment Induced by Repeated Stress.
Wei J, Xiong Z, Lee JB, Cheng J, Duffney LJ, Matas E, Yan Z
The Journal of neuroscience : the official journal of the Society for Neuroscience36(7)2119-30(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that dietary factors can regulate cardiac Scnn1g, Nedd4-2, and Sgk1 activity; here, proanthocyanidin dietary supplement PRO80 blocks aldosterone-dependent up-regulation of Scnn1g, Nedd4-2, and Sgk1 content in cardiac dysfunction. (Scnn1g = epithelial sodium channel 1, gamma subunit; Nedd4-2 = ubiquitin-protein ligase Nedd4-2; Sgk1 = serum-glucocorticoid regulated kinase 1)
Title: Proanthocyanidins block aldosterone-dependent up-regulation of cardiac gamma ENaC and Nedd4-2 inactivation via SGK1.

Structural Studies of the Nedd4 WW Domains and Their Selectivity for the Connexin43 (Cx43) Carboxyl Terminus.
Title: Structural Studies of the Nedd4 WW Domains and Their Selectivity for the Connexin43 (Cx43) Carboxyl Terminus.

histone methyltransferase Ehmt2 (G9a) led to the loss of repressive histone methylation at the Nedd4-1 promoter and the transcriptional activation of Nedd4
Title: Histone Modification of Nedd4 Ubiquitin Ligase Controls the Loss of AMPA Receptors and Cognitive Impairment Induced by Repeated Stress.

CRTC2 and Nedd4 ligase involvement in FSH and TGFbeta1 upregulation of connexin43 gap junction
Title: CRTC2 and Nedd4 ligase involvement in FSH and TGF?1 upregulation of connexin43 gap junction.

In thyroid FRTL-5 cells, activation of the cyclic AMP pathway increases the association of Nedd4 with IRS-2, thereby enhancing IRS-2-mediated signalling and cell proliferation induced by IGF-I.
Title: Nedd4-induced monoubiquitination of IRS-2 enhances IGF signalling and mitogenic activity.

The following Nedd4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nedd4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13421	NM_012986.1 Latest version!	Rattus norvegicus NEDD4 E3 ubiquitin protein ligase (Nedd4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa62324	XM_017595479.1 Latest version!	Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (Nedd4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nedd4 ( NM_012986.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037118.1 Rattus norvegicus NEDD4 E3 ubiquitin protein ligase (Nedd4), mRNA.

Price & Availability↑

CloneID	ORa13421
Clone ID Related Accession (Same CDS sequence)	NM_012986.1
Accession Version	NM_012986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase NEDD4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK365888.1, U50842.1, CB615796.1, CB568921.1, DY312717.1, BQ191927.1, EV775947.1, DY318090.1, CF110862.1 and CB326200.1. On or before Oct 13, 2007 this sequence version replaced XM_343427.3, XM_001053672.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCGG ACATGGCAGC CGACGACACT GAGGCCCCAG TGCTTTCGGA GGACGAGGTA 
TGGGAGTTCT GTCTGGATAA GAATGAAGAG GGTGGTGGAT CCCCCGGAAG TGATGTCACA 
GATACTTGTG AGCCACCATG TGGATGCTGG GAGTTAAATC CGAGTTCCCT GGAAGAGGAG 
CACGTGCTTT TCACTGCCGA TCCATATCTC GAGCTTCACA ATGATGACAC GCGAGTTGTG 
AGAGTGAAGG TTATAGCTGG CATAGGTCTG GCCAAGAAAG ACATCTTGGG AGCCAGTGAT 
CCTTATGTAA GAGTGACATT GTATGACCCG ATGAGTGGAG TCCTTACCAG CGTGCAGACG 
AAAACTATCA AAAAGTCTTT GAATCCAAAA TGGAACGAAG AAATACTGTT CAGAGTCCTT 
CCACAACAGC ACCGGATTCT TTTCGAAGTG TTTGATGAAA ATCGTTTGAC AAGAGATGAT 
TTCCTGGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GCCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTACTTA CCTAAAAACG GCTCAGATGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACC 
CATTTGCAGC ATCCACCAGA ACCTTCTCCC CTACCTCCAG GGTGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAGTCTAGAA CAACACAATG GAAAAGACCA 
AGCCCTGAGG ATGACCTCAC AGATGATGAG AATGGTGACA TTCAGCTGCA GGCGCAGCGA 
GCATTTACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCACGAGTCC 
CCTGAGAATT GGGAGATTGT ACGAGAAGAT GAGAACACCA TGTACAGTGG TCAGGCTGTT 
CAGTCGCCTC CGTCAGGTCA CCCAGACGTG CAGGTCCGCC TCGCAGAGGA GTTAGATACC 
AGACTCACCA TGTATGGAAA CCCGGCCACC AGCCAGCCAG TCACCAGCTC AAACCATTCC 
AGCAGAGGAG GCAGCTCGCA AACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGACGA 
TCGTACTATG TAGACCACAA CTCTAAGACA ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATTCC TGCTCATCTG AGAGGAAAGA CACCAGTCGA CTCCAATGAC 
CTGGGACCTT TACCTCCAGG CTGGGAAGAA AGAACGCACA CAGATGGAAG GGTCTTCTTC 
ATAAATCACA ATATAAAGAA GACACAGTGG GAAGATCCTC GGATGCAGAA TGTGGCCATA 
ACTGGACCAG CAGTGCCCTA CTCCAGAGAT TACAAAAGAA AGTATGAGTT TTTCCGAAGG 
AAGCTGAAGA AGCAGACTGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGTGCAAAC 
ATTCTGGAGG ATTCTTACCG GAGAATTATG GGTGTGAAGA GAGCTGACTT CCTCAAGGCC 
CGACTCTGGA TTGAGTTTGA TGGTGAAAAG GGCCTTGACT ATGGAGGAGT TGCCCGAGAA 
TGGTTCTTCC TCATCTCAAA GGAGATGTTT AATCCCTATT ATGGCCTGTT TGAATATTCC 
GCTACGGACA ATTACACTCT GCAGATAAAT CCAAACTCGG GCTTGTGTAA TGAAGATCAC 
CTCTCATACT TCAAGTTCAT TGGCCGTGTG GCTGGGATGG CGGTTTATCA TGGCAAGCTG 
TTGGATGGGT TTTTCATCCG CCCCTTTTAC AAGATGATGC TTCAGAAACT GATAACACTG 
CACGACATGG AGTCCGTGGA TAGTGAATAT TACAGTTCTC TGCGATGGAT TCTTGAAAAT 
GACCCGACGG AGCTGGACCT GAGATTTATC ATAGATGAAG AGCTTTTTGG ACAGACACAT 
CAACACGAGC TGAAAACTGG TGGATCGGAG GTTGTGGTCA CCAACAAGAA CAAAAAGGAG 
TATATCTACC TTGTAATCCA GTGGCGATTT GTGAACAGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTTTTTGA ACTGATACCA CAGGATCTGA TCAAGATATT CGATGAAAAT 
GAACTAGAGC TTCTCATGTG TGGTCTGGGA GATGTGGACG TGAACGACTG GAGGGAACAT 
ACAAAATACA AAAATGGATA CAGCTTAAAC CACCAGGTCA TCCACTGGTT CTGGAAGGCT 
GTTTTGATGA TGGATTCAGA AAAAAGAATA CGCTTACTTC AGTTTGTTAC TGGCACATCC 
CGTGTGCCTA TGAATGGATT TGCTGAACTC TATGGCTCAA ATGGACCACA GTCCTTCACA 
GTGGAACAGT GGGGCACCCC TGATAAGCTG CCCAGAGCAC ATACCTGCTT CAACCGCCTG 
GACCTGCCAC CCTATGAATC CTTTGACGAA CTCTGGGATA AACTTCAGAT GGCGATTGAG 
AACACGCAGG GCTTTGATGG GGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037118.1
CDS	66..2735
Translation	MSSDMAADDTEAPVLSEDEVWEFCLDKNEEGGGSPGSDVTDTCEPPCGCWELNPSSLEEEHVLFTADPYLELHNDDTRVVRVKVIAGIGLAKKDILGASDPYVRVTLYDPMSGVLTSVQTKTIKKSLNPKWNEEILFRVLPQQHRILFEVFDENRLTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSDDENADQAEELEPGWVVLDQPDAATHLQHPPEPSPLPPGWEERQDVLGRTYYVNHESRTTQWKRPSPEDDLTDDENGDIQLQAQRAFTTRRQISEDVDGPDNHESPENWEIVREDENTMYSGQAVQSPPSGHPDVQVRLAEELDTRLTMYGNPATSQPVTSSNHSSRGGSSQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTPVDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRMQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEVVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSLNHQVIHWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	NM_012986.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NEDD4 E3 ubiquitin protein ligase (Nedd4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012986.1

ORF Insert Sequence:

ATGAGCTCGG ACATGGCAGC CGACGACACT GAGGCCCCAG TGCTTTCGGA GGACGAGGTA 
TGGGAGTTCT GTCTGGATAA GAATGAAGAG GGTGGTGGAT CCCCCGGAAG TGATGTCACA 
GATACTTGTG AGCCACCATG TGGATGCTGG GAGTTAAATC CGAGTTCCCT GGAAGAGGAG 
CACGTGCTTT TCACTGCCGA TCCATATCTC GAGCTTCACA ATGATGACAC GCGAGTTGTG 
AGAGTGAAGG TTATAGCTGG CATAGGTCTG GCCAAGAAAG ACATCTTGGG AGCCAGTGAT 
CCTTATGTAA GAGTGACATT GTATGACCCG ATGAGTGGAG TCCTTACCAG CGTGCAGACG 
AAAACTATCA AAAAGTCTTT GAATCCAAAA TGGAACGAAG AAATACTGTT CAGAGTCCTT 
CCACAACAGC ACCGGATTCT TTTCGAAGTG TTTGATGAAA ATCGTTTGAC AAGAGATGAT 
TTCCTGGGTC AAGTGGATGT CCCTCTCTAT CCTTTACCGA CTGAAAACCC AAGAATGGAG 
AGACCATATA CATTTAAGGA TTTTGTTCTT CATCCAAGAA GCCACAAATC AAGAGTTAAA 
GGTTATCTGA GATTAAAAAT GACTTACTTA CCTAAAAACG GCTCAGATGA TGAAAATGCA 
GACCAGGCTG AGGAGTTAGA GCCTGGCTGG GTTGTTTTGG ACCAACCAGA TGCTGCCACC 
CATTTGCAGC ATCCACCAGA ACCTTCTCCC CTACCTCCAG GGTGGGAAGA GAGGCAGGAT 
GTCCTTGGAA GGACCTACTA CGTAAACCAT GAGTCTAGAA CAACACAATG GAAAAGACCA 
AGCCCTGAGG ATGACCTCAC AGATGATGAG AATGGTGACA TTCAGCTGCA GGCGCAGCGA 
GCATTTACCA CCAGGCGGCA GATATCGGAG GATGTGGATG GCCCTGACAA CCACGAGTCC 
CCTGAGAATT GGGAGATTGT ACGAGAAGAT GAGAACACCA TGTACAGTGG TCAGGCTGTT 
CAGTCGCCTC CGTCAGGTCA CCCAGACGTG CAGGTCCGCC TCGCAGAGGA GTTAGATACC 
AGACTCACCA TGTATGGAAA CCCGGCCACC AGCCAGCCAG TCACCAGCTC AAACCATTCC 
AGCAGAGGAG GCAGCTCGCA AACCTGTATC TTTGAGGAAC AGCCTACACT TCCTGTGCTT 
TTGCCTACTT CATCTGGATT GCCACCAGGT TGGGAAGAAA AACAAGATGA CAGAGGACGA 
TCGTACTATG TAGACCACAA CTCTAAGACA ACCACATGGT CCAAGCCCAC CATGCAGGAT 
GATCCAAGAT CGAAAATTCC TGCTCATCTG AGAGGAAAGA CACCAGTCGA CTCCAATGAC 
CTGGGACCTT TACCTCCAGG CTGGGAAGAA AGAACGCACA CAGATGGAAG GGTCTTCTTC 
ATAAATCACA ATATAAAGAA GACACAGTGG GAAGATCCTC GGATGCAGAA TGTGGCCATA 
ACTGGACCAG CAGTGCCCTA CTCCAGAGAT TACAAAAGAA AGTATGAGTT TTTCCGAAGG 
AAGCTGAAGA AGCAGACTGA CATTCCAAAC AAATTTGAAA TGAAACTTCG CCGTGCAAAC 
ATTCTGGAGG ATTCTTACCG GAGAATTATG GGTGTGAAGA GAGCTGACTT CCTCAAGGCC 
CGACTCTGGA TTGAGTTTGA TGGTGAAAAG GGCCTTGACT ATGGAGGAGT TGCCCGAGAA 
TGGTTCTTCC TCATCTCAAA GGAGATGTTT AATCCCTATT ATGGCCTGTT TGAATATTCC 
GCTACGGACA ATTACACTCT GCAGATAAAT CCAAACTCGG GCTTGTGTAA TGAAGATCAC 
CTCTCATACT TCAAGTTCAT TGGCCGTGTG GCTGGGATGG CGGTTTATCA TGGCAAGCTG 
TTGGATGGGT TTTTCATCCG CCCCTTTTAC AAGATGATGC TTCAGAAACT GATAACACTG 
CACGACATGG AGTCCGTGGA TAGTGAATAT TACAGTTCTC TGCGATGGAT TCTTGAAAAT 
GACCCGACGG AGCTGGACCT GAGATTTATC ATAGATGAAG AGCTTTTTGG ACAGACACAT 
CAACACGAGC TGAAAACTGG TGGATCGGAG GTTGTGGTCA CCAACAAGAA CAAAAAGGAG 
TATATCTACC TTGTAATCCA GTGGCGATTT GTGAACAGAA TCCAGAAGCA AATGGCTGCT 
TTTAAAGAGG GATTTTTTGA ACTGATACCA CAGGATCTGA TCAAGATATT CGATGAAAAT 
GAACTAGAGC TTCTCATGTG TGGTCTGGGA GATGTGGACG TGAACGACTG GAGGGAACAT 
ACAAAATACA AAAATGGATA CAGCTTAAAC CACCAGGTCA TCCACTGGTT CTGGAAGGCT 
GTTTTGATGA TGGATTCAGA AAAAAGAATA CGCTTACTTC AGTTTGTTAC TGGCACATCC 
CGTGTGCCTA TGAATGGATT TGCTGAACTC TATGGCTCAA ATGGACCACA GTCCTTCACA 
GTGGAACAGT GGGGCACCCC TGATAAGCTG CCCAGAGCAC ATACCTGCTT CAACCGCCTG 
GACCTGCCAC CCTATGAATC CTTTGACGAA CTCTGGGATA AACTTCAGAT GGCGATTGAG 
AACACGCAGG GCTTTGATGG GGTTGATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nedd4 ( XM_017595479.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450968.1 Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (Nedd4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62324
Clone ID Related Accession (Same CDS sequence)	XM_017595479.1
Accession Version	XM_017595479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase NEDD4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA GTTTACGATT GCACTTCGCA GCCAGAAGAA GCAAGACTTA CCCTCTGTCG 
GAACCCTCTG GAGATGACTT GGATAACCAC ATTCACACAC ACGGCCAGAG GCCACCCCGG 
ATCTCAGTGG CCAGCGTGGT TCAAATGAGG CTGATCCCCA GACAGACGCC ACTGGCTCAG 
TGGGTAGATC ATAGATCCGC CGAAAGTACT GACAGAAAGA GCAGCTGCCT GCAGATCTCA 
CTGCAGCCCA GGTATGGTGG GTGTCTTCCC TCCGGCACTG TTGTGGTAGA TAGGGACGAT 
ATTTCCTTTA CCTGTGCTTT AAAGGATAGT GTTTGCAGCA GCACTGGAGT TGACAGTGAG 
GTGTCCAGTG CCAGTGATGG CAGCCTCTTA GGTATTTCTG CCATGTACAA TGGCAGCCTC 
AGTAACTTCT CAGCCAGTGA CAATGGGTCT TACAGCAGCA ATGGTAGTGA CTGTGGGTCT 
TGTGCAAGCC TCACAAGTGG ATGCTCTTAC ACAGGCAGTG TTGTCAGCGA CAGTAGTGGT 
TATGAGACCT TTTTCAGTGG AAATTTACCT TCTGACAACA CCTCCAACAG AAGCGTGCCA 
GTCAACACTG CTCCCCGTGA AGTTCTGTGC AGAAGTCCCA GTAGGCTTCC CTTTGCCCAG 
GAAGACCTGG AACATGGAGT AGAGATTATG AAAGTGCCGG TGAGCGGGAA TGCAAAGGTT 
CCACTGAAGC GCTGCACATC TTTGGTCATT TTCCCAAGGA GTCCTTCAAA CACTCCACCA 
GCATCCCCGA CAAGCAGGGG TGCCTACCAG ACCTCACACC AGTTGGTTGT GTCTCCTACC 
GAAACTGCAC ATCATGAAGA CGGAACTGGC ACCAGGGGCT TCCTGTCGAC AGCTGTCAGC 
GGGCTTCGGT TATCTAAAGT GACTTGTACT CCTGGGGAAG TCAGAGATGT TCGGCCGCTC 
CATGCCAAGG GCTCCCTGCA AAAGAAAATT GTCATTTCAG ATAATAGGCC CAAACAAACT 
GTGTGTGAGA AGTTGCCGGA AAGATTTTCC TGTGTTTCTG TGCACTTGAC CCAACAAAAT 
CAAACAGCGT CAGATTGTGC AAACCCATGT GTTGGCCGCA CATCAGAGAG GTTAGAACTT 
CAGCTCCAGT CTGATTTGGA CCATGGCATG CTCACACAAA GGGCATCTCC TTGCTTACCA 
ACTACACCAG ACACGGATTC CCATGTAAAC ATCCGTGGAG AGTTGGAAAG ACCAAATTCA 
CAGAGAAGCA GAGGCCGTGC TGCTTTGCAA AGAAGTGTCT CGCTGGGTGG GACATATCCG 
AATGTTTCCT GTTTATCCAG CCTGAAGCAC AGTTGTTCAA AGGGGGGACC ATCTCAGTTG 
CTCATAAAGT TTGCATCTGG GAATGACGGT AAAGTTGATA GTTTCTGGAG AGAGAGAGAC 
TTGACAAACG AACTGTCCGG TGTCCGTAAG ACAAGAGATG ATTTCCTGGG TCAAGTGGAT 
GTCCCTCTCT ATCCTTTACC GACTGAAAAC CCAAGAATGG AGAGACCATA TACATTTAAG 
GATTTTGTTC TTCATCCAAG AAGCCACAAA TCAAGAGTTA AAGGTTATCT GAGATTAAAA 
ATGACTTACT TACCTAAAAA CGGCTCAGAT GATGAAAATG CAGACCAGGC TGAGGAGTTA 
GAGCCTGGCT GGGTTGTTTT GGACCAACCA GATGCTGCCA CCCATTTGCA GCATCCACCA 
GAACCTTCTC CCCTACCTCC AGGGTGGGAA GAGAGGCAGG ATGTCCTTGG AAGGACCTAC 
TACGTAAACC ATGAGTCTAG AACAACACAA TGGAAAAGAC CAAGCCCTGA GGATGACCTC 
ACAGATGATG AGAATGGTGA CATTCAGCTG CAGGCGCAGC GAGCATTTAC CACCAGGCGG 
CAGATATCGG AGGATGTGGA TGGCCCTGAC AACCACGAGT CCCCTGAGAA TTGGGAGATT 
GTACGAGAAG ATGAGAACAC CATGTACAGT GGTCAGGCTG TTCAGTCGCC TCCGTCAGGT 
CACCCAGACG TGCAGGTCCG CCTCGCAGAG GAGTTAGATA CCAGACTCAC CATGTATGGA 
AACCCGGCCA CCAGCCAGCC AGTCACCAGC TCAAACCATT CCAGCAGAGG AGGCAGCTCG 
CAAACCTGTA TCTTTGAGGA ACAGCCTACA CTTCCTGTGC TTTTGCCTAC TTCATCTGGA 
TTGCCACCAG GTTGGGAAGA AAAACAAGAT GACAGAGGAC GATCGTACTA TGTAGACCAC 
AACTCTAAGA CAACCACATG GTCCAAGCCC ACCATGCAGG ATGATCCAAG ATCGAAAATT 
CCTGCTCATC TGAGAGGAAA GACACCAGTC GACTCCAATG ACCTGGGACC TTTACCTCCA 
GGCTGGGAAG AAAGAACGCA CACAGATGGA AGGGTCTTCT TCATAAATCA CAATATAAAG 
AAGACACAGT GGGAAGATCC TCGGATGCAG AATGTGGCCA TAACTGGACC AGCAGTGCCC 
TACTCCAGAG ATTACAAAAG AAAGTATGAG TTTTTCCGAA GGAAGCTGAA GAAGCAGACT 
GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGTGCAA ACATTCTGGA GGATTCTTAC 
CGGAGAATTA TGGGTGTGAA GAGAGCTGAC TTCCTCAAGG CCCGACTCTG GATTGAGTTT 
GATGGTGAAA AGGGCCTTGA CTATGGAGGA GTTGCCCGAG AATGGTTCTT CCTCATCTCA 
AAGGAGATGT TTAATCCCTA TTATGGCCTG TTTGAATATT CCGCTACGGA CAATTACACT 
CTGCAGATAA ATCCAAACTC GGGCTTGTGT AATGAAGATC ACCTCTCATA CTTCAAGTTC 
ATTGGCCGTG TGGCTGGGAT GGCGGTTTAT CATGGCAAGC TGTTGGATGG GTTTTTCATC 
CGCCCCTTTT ACAAGATGAT GCTTCAGAAA CTGATAACAC TGCACGACAT GGAGTCCGTG 
GATAGTGAAT ATTACAGTTC TCTGCGATGG ATTCTTGAAA ATGACCCGAC GGAGCTGGAC 
CTGAGATTTA TCATAGATGA AGAGCTTTTT GGACAGACAC ATCAACACGA GCTGAAAACT 
GGTGGATCGG AGGTTGTGGT CACCAACAAG AACAAAAAGG AGTATATCTA CCTTGTAATC 
CAGTGGCGAT TTGTGAACAG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTTTTT 
GAACTGATAC CACAGGATCT GATCAAGATA TTCGATGAAA ATGAACTAGA GCTTCTCATG 
TGTGGTCTGG GAGATGTGGA CGTGAACGAC TGGAGGGAAC ATACAAAATA CAAAAATGGA 
TACAGCTTAA ACCACCAGGT CATCCACTGG TTCTGGAAGG CTGTTTTGAT GATGGATTCA 
GAAAAAAGAA TACGCTTACT TCAGTTTGTT ACTGGCACAT CCCGTGTGCC TATGAATGGA 
TTTGCTGAAC TCTATGGCTC AAATGGACCA CAGTCCTTCA CAGTGGAACA GTGGGGCACC 
CCTGATAAGC TGCCCAGAGC ACATACCTGC TTCAACCGCC TGGACCTGCC ACCCTATGAA 
TCCTTTGACG AACTCTGGGA TAAACTTCAG ATGGCGATTG AGAACACGCA GGGCTTTGAT 
GGGGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450968.1
CDS	2450..6121
Translation	MAQSLRLHFAARRSKTYPLSEPSGDDLDNHIHTHGQRPPRISVASVVQMRLIPRQTPLAQWVDHRSAESTDRKSSCLQISLQPRYGGCLPSGTVVVDRDDISFTCALKDSVCSSTGVDSEVSSASDGSLLGISAMYNGSLSNFSASDNGSYSSNGSDCGSCASLTSGCSYTGSVVSDSSGYETFFSGNLPSDNTSNRSVPVNTAPREVLCRSPSRLPFAQEDLEHGVEIMKVPVSGNAKVPLKRCTSLVIFPRSPSNTPPASPTSRGAYQTSHQLVVSPTETAHHEDGTGTRGFLSTAVSGLRLSKVTCTPGEVRDVRPLHAKGSLQKKIVISDNRPKQTVCEKLPERFSCVSVHLTQQNQTASDCANPCVGRTSERLELQLQSDLDHGMLTQRASPCLPTTPDTDSHVNIRGELERPNSQRSRGRAALQRSVSLGGTYPNVSCLSSLKHSCSKGGPSQLLIKFASGNDGKVDSFWRERDLTNELSGVRKTRDDFLGQVDVPLYPLPTENPRMERPYTFKDFVLHPRSHKSRVKGYLRLKMTYLPKNGSDDENADQAEELEPGWVVLDQPDAATHLQHPPEPSPLPPGWEERQDVLGRTYYVNHESRTTQWKRPSPEDDLTDDENGDIQLQAQRAFTTRRQISEDVDGPDNHESPENWEIVREDENTMYSGQAVQSPPSGHPDVQVRLAEELDTRLTMYGNPATSQPVTSSNHSSRGGSSQTCIFEEQPTLPVLLPTSSGLPPGWEEKQDDRGRSYYVDHNSKTTTWSKPTMQDDPRSKIPAHLRGKTPVDSNDLGPLPPGWEERTHTDGRVFFINHNIKKTQWEDPRMQNVAITGPAVPYSRDYKRKYEFFRRKLKKQTDIPNKFEMKLRRANILEDSYRRIMGVKRADFLKARLWIEFDGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLQKLITLHDMESVDSEYYSSLRWILENDPTELDLRFIIDEELFGQTHQHELKTGGSEVVVTNKNKKEYIYLVIQWRFVNRIQKQMAAFKEGFFELIPQDLIKIFDENELELLMCGLGDVDVNDWREHTKYKNGYSLNHQVIHWFWKAVLMMDSEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQSFTVEQWGTPDKLPRAHTCFNRLDLPPYESFDELWDKLQMAIENTQGFDGVD

Target ORF information:

RefSeq Version	XM_017595479.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (Nedd4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595479.1

ORF Insert Sequence:

ATGGCACAGA GTTTACGATT GCACTTCGCA GCCAGAAGAA GCAAGACTTA CCCTCTGTCG 
GAACCCTCTG GAGATGACTT GGATAACCAC ATTCACACAC ACGGCCAGAG GCCACCCCGG 
ATCTCAGTGG CCAGCGTGGT TCAAATGAGG CTGATCCCCA GACAGACGCC ACTGGCTCAG 
TGGGTAGATC ATAGATCCGC CGAAAGTACT GACAGAAAGA GCAGCTGCCT GCAGATCTCA 
CTGCAGCCCA GGTATGGTGG GTGTCTTCCC TCCGGCACTG TTGTGGTAGA TAGGGACGAT 
ATTTCCTTTA CCTGTGCTTT AAAGGATAGT GTTTGCAGCA GCACTGGAGT TGACAGTGAG 
GTGTCCAGTG CCAGTGATGG CAGCCTCTTA GGTATTTCTG CCATGTACAA TGGCAGCCTC 
AGTAACTTCT CAGCCAGTGA CAATGGGTCT TACAGCAGCA ATGGTAGTGA CTGTGGGTCT 
TGTGCAAGCC TCACAAGTGG ATGCTCTTAC ACAGGCAGTG TTGTCAGCGA CAGTAGTGGT 
TATGAGACCT TTTTCAGTGG AAATTTACCT TCTGACAACA CCTCCAACAG AAGCGTGCCA 
GTCAACACTG CTCCCCGTGA AGTTCTGTGC AGAAGTCCCA GTAGGCTTCC CTTTGCCCAG 
GAAGACCTGG AACATGGAGT AGAGATTATG AAAGTGCCGG TGAGCGGGAA TGCAAAGGTT 
CCACTGAAGC GCTGCACATC TTTGGTCATT TTCCCAAGGA GTCCTTCAAA CACTCCACCA 
GCATCCCCGA CAAGCAGGGG TGCCTACCAG ACCTCACACC AGTTGGTTGT GTCTCCTACC 
GAAACTGCAC ATCATGAAGA CGGAACTGGC ACCAGGGGCT TCCTGTCGAC AGCTGTCAGC 
GGGCTTCGGT TATCTAAAGT GACTTGTACT CCTGGGGAAG TCAGAGATGT TCGGCCGCTC 
CATGCCAAGG GCTCCCTGCA AAAGAAAATT GTCATTTCAG ATAATAGGCC CAAACAAACT 
GTGTGTGAGA AGTTGCCGGA AAGATTTTCC TGTGTTTCTG TGCACTTGAC CCAACAAAAT 
CAAACAGCGT CAGATTGTGC AAACCCATGT GTTGGCCGCA CATCAGAGAG GTTAGAACTT 
CAGCTCCAGT CTGATTTGGA CCATGGCATG CTCACACAAA GGGCATCTCC TTGCTTACCA 
ACTACACCAG ACACGGATTC CCATGTAAAC ATCCGTGGAG AGTTGGAAAG ACCAAATTCA 
CAGAGAAGCA GAGGCCGTGC TGCTTTGCAA AGAAGTGTCT CGCTGGGTGG GACATATCCG 
AATGTTTCCT GTTTATCCAG CCTGAAGCAC AGTTGTTCAA AGGGGGGACC ATCTCAGTTG 
CTCATAAAGT TTGCATCTGG GAATGACGGT AAAGTTGATA GTTTCTGGAG AGAGAGAGAC 
TTGACAAACG AACTGTCCGG TGTCCGTAAG ACAAGAGATG ATTTCCTGGG TCAAGTGGAT 
GTCCCTCTCT ATCCTTTACC GACTGAAAAC CCAAGAATGG AGAGACCATA TACATTTAAG 
GATTTTGTTC TTCATCCAAG AAGCCACAAA TCAAGAGTTA AAGGTTATCT GAGATTAAAA 
ATGACTTACT TACCTAAAAA CGGCTCAGAT GATGAAAATG CAGACCAGGC TGAGGAGTTA 
GAGCCTGGCT GGGTTGTTTT GGACCAACCA GATGCTGCCA CCCATTTGCA GCATCCACCA 
GAACCTTCTC CCCTACCTCC AGGGTGGGAA GAGAGGCAGG ATGTCCTTGG AAGGACCTAC 
TACGTAAACC ATGAGTCTAG AACAACACAA TGGAAAAGAC CAAGCCCTGA GGATGACCTC 
ACAGATGATG AGAATGGTGA CATTCAGCTG CAGGCGCAGC GAGCATTTAC CACCAGGCGG 
CAGATATCGG AGGATGTGGA TGGCCCTGAC AACCACGAGT CCCCTGAGAA TTGGGAGATT 
GTACGAGAAG ATGAGAACAC CATGTACAGT GGTCAGGCTG TTCAGTCGCC TCCGTCAGGT 
CACCCAGACG TGCAGGTCCG CCTCGCAGAG GAGTTAGATA CCAGACTCAC CATGTATGGA 
AACCCGGCCA CCAGCCAGCC AGTCACCAGC TCAAACCATT CCAGCAGAGG AGGCAGCTCG 
CAAACCTGTA TCTTTGAGGA ACAGCCTACA CTTCCTGTGC TTTTGCCTAC TTCATCTGGA 
TTGCCACCAG GTTGGGAAGA AAAACAAGAT GACAGAGGAC GATCGTACTA TGTAGACCAC 
AACTCTAAGA CAACCACATG GTCCAAGCCC ACCATGCAGG ATGATCCAAG ATCGAAAATT 
CCTGCTCATC TGAGAGGAAA GACACCAGTC GACTCCAATG ACCTGGGACC TTTACCTCCA 
GGCTGGGAAG AAAGAACGCA CACAGATGGA AGGGTCTTCT TCATAAATCA CAATATAAAG 
AAGACACAGT GGGAAGATCC TCGGATGCAG AATGTGGCCA TAACTGGACC AGCAGTGCCC 
TACTCCAGAG ATTACAAAAG AAAGTATGAG TTTTTCCGAA GGAAGCTGAA GAAGCAGACT 
GACATTCCAA ACAAATTTGA AATGAAACTT CGCCGTGCAA ACATTCTGGA GGATTCTTAC 
CGGAGAATTA TGGGTGTGAA GAGAGCTGAC TTCCTCAAGG CCCGACTCTG GATTGAGTTT 
GATGGTGAAA AGGGCCTTGA CTATGGAGGA GTTGCCCGAG AATGGTTCTT CCTCATCTCA 
AAGGAGATGT TTAATCCCTA TTATGGCCTG TTTGAATATT CCGCTACGGA CAATTACACT 
CTGCAGATAA ATCCAAACTC GGGCTTGTGT AATGAAGATC ACCTCTCATA CTTCAAGTTC 
ATTGGCCGTG TGGCTGGGAT GGCGGTTTAT CATGGCAAGC TGTTGGATGG GTTTTTCATC 
CGCCCCTTTT ACAAGATGAT GCTTCAGAAA CTGATAACAC TGCACGACAT GGAGTCCGTG 
GATAGTGAAT ATTACAGTTC TCTGCGATGG ATTCTTGAAA ATGACCCGAC GGAGCTGGAC 
CTGAGATTTA TCATAGATGA AGAGCTTTTT GGACAGACAC ATCAACACGA GCTGAAAACT 
GGTGGATCGG AGGTTGTGGT CACCAACAAG AACAAAAAGG AGTATATCTA CCTTGTAATC 
CAGTGGCGAT TTGTGAACAG AATCCAGAAG CAAATGGCTG CTTTTAAAGA GGGATTTTTT 
GAACTGATAC CACAGGATCT GATCAAGATA TTCGATGAAA ATGAACTAGA GCTTCTCATG 
TGTGGTCTGG GAGATGTGGA CGTGAACGAC TGGAGGGAAC ATACAAAATA CAAAAATGGA 
TACAGCTTAA ACCACCAGGT CATCCACTGG TTCTGGAAGG CTGTTTTGAT GATGGATTCA 
GAAAAAAGAA TACGCTTACT TCAGTTTGTT ACTGGCACAT CCCGTGTGCC TATGAATGGA 
TTTGCTGAAC TCTATGGCTC AAATGGACCA CAGTCCTTCA CAGTGGAACA GTGGGGCACC 
CCTGATAAGC TGCCCAGAGC ACATACCTGC TTCAACCGCC TGGACCTGCC ACCCTATGAA 
TCCTTTGACG AACTCTGGGA TAAACTTCAG ATGGCGATTG AGAACACGCA GGGCTTTGAT 
GGGGTTGATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NEDD4-1 protects against ischaemia/reperfusion-induced cardiomyocyte apoptosis via the PI3K/Akt pathway.
Apoptosis : an international journal on programmed cell death22(3)437-448(2017 Mar)
Hu W,Zhang P,Gu J,Yu Q,Zhang D

Proanthocyanidins block aldosterone-dependent up-regulation of cardiac gamma ENaC and Nedd4-2 inactivation via SGK1.
The Journal of nutritional biochemistry3713-19(2016 11)
Galiana-Simal A,Olivares-?lvaro E,Klett-Mingo M,Ruiz-Roso MB,Ballesteros S,de Las Heras N,Fuller PJ,Lahera V,Mart?n-Fern?ndez B

Structural Studies of the Nedd4 WW Domains and Their Selectivity for the Connexin43 (Cx43) Carboxyl Terminus.
The Journal of biological chemistry291(14)7637-50(2016 Apr)
Spagnol G,Kieken F,Kopanic JL,Li H,Zach S,Stauch KL,Grosely R,Sorgen PL

Histone Modification of Nedd4 Ubiquitin Ligase Controls the Loss of AMPA Receptors and Cognitive Impairment Induced by Repeated Stress.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(7)2119-30(2016 Feb)
Wei J,Xiong Z,Lee JB,Cheng J,Duffney LJ,Matas E,Yan Z

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Nedd4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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