Vps35 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vps35
Entrez Gene ID	25479
Full Name	VPS35 retromer complex component
Synonyms	Mem3
General protein information	Preferred Names VPS35 retromer complex component Names vacuolar protein sorting-associated protein 35 Maternal embryonic message 3 vacuolar protein sorting 35 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	mouse homolog is a hydrophilic membrane protein having a direct role in the retrieval of cargo proteins [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105718.2	NP_001099188.2	vacuolar protein sorting-associated protein 35

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS35	NP_060676.2
Canis lupus familiaris (dog)	VPS35	XP_532570.3
Eremothecium gossypii	AGOS_AEL107W	NP_984754.1
Arabidopsis thaliana (thale cress)	VPS35B	NP_177713.3
Rattus norvegicus (Norway rat)	Vps35	NP_001099188.2
Danio rerio (zebrafish)	vps35	NP_001020688.2
	VPS35	XP_001108828.2
Gallus gallus (chicken)	VPS35	NP_001005842.1
Caenorhabditis elegans (roundworm)	vps-35	NP_495180.2
Saccharomyces cerevisiae (baker's yeast)	VPS35	NP_012381.1
Kluyveromyces lactis	KLLA0B07535g	XP_451864.1
Magnaporthe oryzae (rice blast fungus)	MGG_05089	XP_003712611.1
Bos taurus (cattle)	VPS35	NP_001039723.1
Drosophila melanogaster (fruit fly)	Vps35	NP_611651.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007683	XP_308188.4
Schizosaccharomyces pombe (fission yeast)	vps35	NP_588260.2
Oryza sativa (rice)	Os03g0801600	NP_001051593.1
Pan troglodytes (chimpanzee)	VPS35	XP_001161536.1
Mus musculus (house mouse)	Vps35	NP_075373.1
Neurospora crassa	NCU06284	XP_962546.1

Gene Ontology
Bibliography

Related articles in PubMed

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
McNally KE, Faulkner R, Steinberg F, Gallon M, Ghai R, Pim D, Langton P, Pearson N, Danson CM, N?gele H, Morris LL, Singla A, Overlee BL, Heesom KJ, Sessions R, Banks L, Collins BM, Berger I, Billadeau DD, Burstein E, Cullen PJ
Nature cell biology19(10)1214-1225(2017 Oct)

The Retromer Supports AMPA Receptor Trafficking During LTP.
Temkin P, Morishita W, Goswami D, Arendt K, Chen L, Malenka R
Neuron94(1)74-82.e5(2017 Apr)

Depletion of vacuolar protein sorting-associated protein 35 is associated with increased lysosomal degradation of aquaporin-2.
Lee MS, Choi HJ, Park EJ, Park HJ, Kwon TH
American journal of physiology. Renal physiology311(6)F1294-F1307(2016 Dec)

VPS35 regulates cell surface recycling and signaling of dopamine receptor D1.
Wang C, Niu M, Zhou Z, Zheng X, Zhang L, Tian Y, Yu X, Bu G, Xu H, Ma Q, Zhang YW
Neurobiology of aging4622-31(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our results show that Vps35 interacts with AQP2c, and depletion of Vps35 is likely to be associated with decreased AQP2 trafficking and increased lysosomal degradation of AQP2 protein.
Title: Depletion of vacuolar protein sorting-associated protein 35 is associated with increased lysosomal degradation of aquaporin-2.

these data are consistent with roles of Vps35 and Nbea in the retrieval and post-Golgi trafficking of synaptic glycine receptor beta-subunit and possibly other neurotransmitter receptors.
Title: The trafficking proteins Vacuolar Protein Sorting 35 and Neurobeachin interact with the glycine receptor ?-subunit.

rat Vps35 dominant negative behavior in yeast requires a conserved PRLYL motif involved in retromer assembly
Title: Dominant-negative behavior of mammalian Vps35 in yeast requires a conserved PRLYL motif involved in retromer assembly.

The following Vps35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vps35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11906	NM_001105718.2 Latest version!	Rattus norvegicus VPS35 retromer complex component (Vps35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$468.30 $669.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vps35 ( NM_001105718.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099188.2 Rattus norvegicus VPS35 retromer complex component (Vps35), mRNA.

Price & Availability↑

CloneID	ORa11906
Clone ID Related Accession (Same CDS sequence)	NM_001105718.2
Accession Version	NM_001105718.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	vacuolar protein sorting-associated protein 35
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07043339.1, CB583538.1, CB732662.1, CV120410.1, CK596387.1, EV778075.1, CV106736.1, DV725274.1 and BP475974.1. On Sep 20, 2008 this sequence version replaced NM_001105718.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTACAA CACAGCAATC ACCCCAGGAT GAGCAAGAAA AACTCTTGGA TGAAGCCATC 
CAGGCTGTGA AAGTTCAATC ATTCCAGATG AAAAGATGCC TGGACAAAAA CAAGCTGATG 
GACGCTCTGA AACATGCCTC GAATATGCTT GGAGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCTATT TCTGATGAGC TGCACTACTT GGAGGTCTAT 
TTGACTGATG AATTTGCTAA AGGAAGAAAG GTGGCAGATC TCTATGAACT TGTACAGTAT 
GCTGGAAACA TTATTCCAAG GCTTTATCTC TTGATCACAG TTGGAGTTGT GTATGTCAAG 
TCATTTCCTC AGTCCAGGAA AGATATTCTG AAAGATTTGG TAGAAATGTG CCGTGGCGTG 
CAGCATCCAC TAAGGGGTTT GTTTCTTCGA AATTATCTTC TTCAGTGTAC TAGGAACATT 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGATATCAG TGATTCCATG 
GATTTTGTAC TACTCAACTT TGCAGAAATG AATAAGCTCT GGGTGCGGAT GCAGCATCAA 
GGACATAGTC GAGATAGAGA AAAAAGAGAA CGAGAGAGAC AAGAACTGAG AATTTTAGTG 
GGAACTAATT TGGTGCGCCT TAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTAA CAGGCATTTT GGAGCAAGTT GTGAATTGTA GAGATGCTTT GGCTCAAGAG 
TATCTCATGG AGTGCATCAT TCAGGTTTTT CCTGATGAAT TCCATCTCCA GACTTTGAAT 
CCTTTTCTTA GAGCTTGTGC TGAGTTACAC CAAAATGTAA ATGTGAAAAA CATAATCATT 
GCTTTAATTG ACAGATTAGC TTTATTTGCT CATCGTGAAG ATGGACCTGG AATTCCAGCT 
GAGATTAAAC TTTTTGACAT ATTTTCACAA CAGGTGGCTA CAGTGATACA GTCCAGACAA 
GACATGCCAT CAGAGGACGT TGTATCTTTA CAAGTCTCTC TCATTAATCT TGCTATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAACTTA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA GCTTACCAGA 
CTTTTGAAGA TTCCAGTTGA TACTTACAAC AATATCTTAA CAGTCTTAAA GTTAAAGCAT 
TTCCACCCAC TTTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG CTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATCGTCTCTC AGGACCAGGT AGATTCCATA 
ATGAATTTGG TGTCCACGTT GATTCAGGAT CAGCCAGACC AGCCTGTAGA AGACCCTGAC 
CCAGAAGACT TTGCTGATGA ACAGAGCCTT GTTGGCCGAT TTATTCACCT TCTGCGTTCT 
GAAGACCCTG ATCAGCAGTA TTTGATTTTG AATACAGCAC GAAAACATTT TGGGGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA TCAGTTGGCT 
TTTCGGTACA AAGAGAATTC TCAAATGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCATCAGAC TATCAGTGCT TTGATTAAAG CCGAGCTGGC TGAGTTACCA 
CTGAGACTTT TTCTTCAAGG AGCATTAGCT GCTGGGGAGA TTGGCTTCGA AAATCATGAA 
ACAGTAGCAT ATGAATTTAT GTCCCAGGCG TTTTCTCTAT ATGAGGATGA ACTCAGTGAC 
TCCAAAGCAC AGCTGGCTGC TATCACTTTG ATCATTGGTA CTTTTGAGAG GATGAAATGC 
TTCAGTGAAG AGAATCATGA GCCCTTGAGA ACTCAGTGTG CACTTGCTGC ATCAAAACTT 
CTGAAAAAAC CGGACCAAGG CCGAGCTGTG AGCACATGTG CACATCTCTT TTGGTCTGGC 
CGAAACACAG ACAAAAATGG GGAAGAGCTT CATGGAGGTA AAAGGGTCAT GGAGTGCCTA 
AAGAAGGCAC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT TCAGCTCTTT 
ATAGAGATTC TGAACAGGTA CATCTATTTC TATGAAAAAG AAAATGATGC GGTAACAATT 
CAGGTCTTGA ACCAACTTAT TCAAAAAATT CGAGAAGATC TCCCAAACCT TGAGTCCAGT 
GAAGAAACAG AGCAAATTAA CAAGCATTTT CACAACACGT TGGAGCACTT GCGCTCAAGA 
CGGGAATCAC CAGAGTCTGA GGGGCCAATC TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099188.2
CDS	61..2451
Translation	MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSQMDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDELSDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRSRRESPESEGPIYEGLIL

Target ORF information:

RefSeq Version	NM_001105718.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus VPS35 retromer complex component (Vps35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105718.2

ORF Insert Sequence:

ATGCCTACAA CACAGCAATC ACCCCAGGAT GAGCAAGAAA AACTCTTGGA TGAAGCCATC 
CAGGCTGTGA AAGTTCAATC ATTCCAGATG AAAAGATGCC TGGACAAAAA CAAGCTGATG 
GACGCTCTGA AACATGCCTC GAATATGCTT GGAGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCTATT TCTGATGAGC TGCACTACTT GGAGGTCTAT 
TTGACTGATG AATTTGCTAA AGGAAGAAAG GTGGCAGATC TCTATGAACT TGTACAGTAT 
GCTGGAAACA TTATTCCAAG GCTTTATCTC TTGATCACAG TTGGAGTTGT GTATGTCAAG 
TCATTTCCTC AGTCCAGGAA AGATATTCTG AAAGATTTGG TAGAAATGTG CCGTGGCGTG 
CAGCATCCAC TAAGGGGTTT GTTTCTTCGA AATTATCTTC TTCAGTGTAC TAGGAACATT 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGATATCAG TGATTCCATG 
GATTTTGTAC TACTCAACTT TGCAGAAATG AATAAGCTCT GGGTGCGGAT GCAGCATCAA 
GGACATAGTC GAGATAGAGA AAAAAGAGAA CGAGAGAGAC AAGAACTGAG AATTTTAGTG 
GGAACTAATT TGGTGCGCCT TAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTAA CAGGCATTTT GGAGCAAGTT GTGAATTGTA GAGATGCTTT GGCTCAAGAG 
TATCTCATGG AGTGCATCAT TCAGGTTTTT CCTGATGAAT TCCATCTCCA GACTTTGAAT 
CCTTTTCTTA GAGCTTGTGC TGAGTTACAC CAAAATGTAA ATGTGAAAAA CATAATCATT 
GCTTTAATTG ACAGATTAGC TTTATTTGCT CATCGTGAAG ATGGACCTGG AATTCCAGCT 
GAGATTAAAC TTTTTGACAT ATTTTCACAA CAGGTGGCTA CAGTGATACA GTCCAGACAA 
GACATGCCAT CAGAGGACGT TGTATCTTTA CAAGTCTCTC TCATTAATCT TGCTATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAACTTA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA GCTTACCAGA 
CTTTTGAAGA TTCCAGTTGA TACTTACAAC AATATCTTAA CAGTCTTAAA GTTAAAGCAT 
TTCCACCCAC TTTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG CTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATCGTCTCTC AGGACCAGGT AGATTCCATA 
ATGAATTTGG TGTCCACGTT GATTCAGGAT CAGCCAGACC AGCCTGTAGA AGACCCTGAC 
CCAGAAGACT TTGCTGATGA ACAGAGCCTT GTTGGCCGAT TTATTCACCT TCTGCGTTCT 
GAAGACCCTG ATCAGCAGTA TTTGATTTTG AATACAGCAC GAAAACATTT TGGGGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA TCAGTTGGCT 
TTTCGGTACA AAGAGAATTC TCAAATGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCATCAGAC TATCAGTGCT TTGATTAAAG CCGAGCTGGC TGAGTTACCA 
CTGAGACTTT TTCTTCAAGG AGCATTAGCT GCTGGGGAGA TTGGCTTCGA AAATCATGAA 
ACAGTAGCAT ATGAATTTAT GTCCCAGGCG TTTTCTCTAT ATGAGGATGA ACTCAGTGAC 
TCCAAAGCAC AGCTGGCTGC TATCACTTTG ATCATTGGTA CTTTTGAGAG GATGAAATGC 
TTCAGTGAAG AGAATCATGA GCCCTTGAGA ACTCAGTGTG CACTTGCTGC ATCAAAACTT 
CTGAAAAAAC CGGACCAAGG CCGAGCTGTG AGCACATGTG CACATCTCTT TTGGTCTGGC 
CGAAACACAG ACAAAAATGG GGAAGAGCTT CATGGAGGTA AAAGGGTCAT GGAGTGCCTA 
AAGAAGGCAC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT TCAGCTCTTT 
ATAGAGATTC TGAACAGGTA CATCTATTTC TATGAAAAAG AAAATGATGC GGTAACAATT 
CAGGTCTTGA ACCAACTTAT TCAAAAAATT CGAGAAGATC TCCCAAACCT TGAGTCCAGT 
GAAGAAACAG AGCAAATTAA CAAGCATTTT CACAACACGT TGGAGCACTT GCGCTCAAGA 
CGGGAATCAC CAGAGTCTGA GGGGCCAATC TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
Nature cell biology19(10)1214-1225(2017 Oct)
McNally KE,Faulkner R,Steinberg F,Gallon M,Ghai R,Pim D,Langton P,Pearson N,Danson CM,N?gele H,Morris LL,Singla A,Overlee BL,Heesom KJ,Sessions R,Banks L,Collins BM,Berger I,Billadeau DD,Burstein E,Cullen PJ

The Retromer Supports AMPA Receptor Trafficking During LTP.
Neuron94(1)74-82.e5(2017 Apr)
Temkin P,Morishita W,Goswami D,Arendt K,Chen L,Malenka R

Depletion of vacuolar protein sorting-associated protein 35 is associated with increased lysosomal degradation of aquaporin-2.
American journal of physiology. Renal physiology311(6)F1294-F1307(2016 Dec)
Lee MS,Choi HJ,Park EJ,Park HJ,Kwon TH

VPS35 regulates cell surface recycling and signaling of dopamine receptor D1.
Neurobiology of aging4622-31(2016 Oct)
Wang C,Niu M,Zhou Z,Zheng X,Zhang L,Tian Y,Yu X,Bu G,Xu H,Ma Q,Zhang YW