Gss cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gss
Entrez Gene ID	25458
Full Name	glutathione synthetase
General protein information	Preferred Names glutathione synthetase Names glutathione synthetase GSH synthetase GSH-S glutathione synthase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	catalyzes the synthesis of glutathione from gamma-L-glutamyl-L-cysteine and glycine; activity is ATP-dependent [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012962.1	NP_037094.1	glutathione synthetase

Pathways from BioSystems

Homologies

Homo sapiens (human)	GSS	NP_000169.1
Rattus norvegicus (Norway rat)	Gss	NP_037094.1
Drosophila melanogaster (fruit fly)	CG32495	NP_728116.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000534	XP_310552.5
Magnaporthe oryzae (rice blast fungus)	MGG_06454	XP_003717112.1
Canis lupus familiaris (dog)	GSS	XP_005635021.1
Oryza sativa (rice)	Os11g0642800	NP_001068356.1
Pan troglodytes (chimpanzee)	GSS	XP_003953759.1
Drosophila melanogaster (fruit fly)	GS	NP_001162782.1
Saccharomyces cerevisiae (baker's yeast)	GSH2	NP_014593.1
Kluyveromyces lactis	KLLA0F07557g	XP_455422.1
Bos taurus (cattle)	GSS	NP_001015630.1
Gallus gallus (chicken)	GSS	XP_004947034.1
Caenorhabditis elegans (roundworm)	gss-1	NP_496011.1
Eremothecium gossypii	AGOS_ACR284C	NP_983686.1
Schizosaccharomyces pombe (fission yeast)	gsa1	NP_593936.1
Neurospora crassa	NCU06191	XP_963003.1
Mus musculus (house mouse)	Gss	NP_032206.1
Danio rerio (zebrafish)	gss	NP_001006104.1
Arabidopsis thaliana (thale cress)	GSH2	NP_568495.2
Xenopus tropicalis (tropical clawed frog)	gss	NP_001008045.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Toward an understanding of the protein interaction network of the human liver.
Wang J, Huo K, Ma L, Tang L, Li D, Huang X, Yuan Y, Li C, Wang W, Guan W, Chen H, Jin C, Wei J, Zhang W, Yang Y, Liu Q, Zhou Y, Zhang C, Wu Z, Xu W, Zhang Y, Liu T, Yu D, Zhang Y, Chen L, Zhu D, Zhong X, Kang L, Gan X, Yu X, Ma Q, Yan J, Zhou L, Liu Z, Zhu Y, Zhou T, He F, Yang X
Molecular systems biology7536(2011 Oct)

High fat feeding and dietary L-arginine supplementation differentially regulate gene expression in rat white adipose tissue.
Jobgen W, Fu WJ, Gao H, Li P, Meininger CJ, Smith SB, Spencer TE, Wu G
Amino acids37(1)187-98(2009 May)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Coordinated changes in xenobiotic metabolizing enzyme gene expression in aging male rats.
Lee JS, Ward WO, Wolf DC, Allen JW, Mills C, DeVito MJ, Corton JC
Toxicological sciences : an official journal of the Society of Toxicology106(1)263-83(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

role of AP-1 in induction by tert-butylhydroquinone
Title: Role of AP-1 in the coordinate induction of rat glutamate-cysteine ligase and glutathione synthetase by tert-butylhydroquinone.

The following Gss gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gss cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01484	NM_012962.1 Latest version!	Rattus norvegicus glutathione synthetase (Gss), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gss ( NM_012962.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037094.1 Rattus norvegicus glutathione synthetase (Gss), mRNA.

Price & Availability↑

CloneID	ORa01484
Clone ID Related Accession (Same CDS sequence)	NM_012962.1
Accession Version	NM_012962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glutathione synthetase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L38615.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L38615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCA GCTGGGGAAG CATCTTGCAG GATGAGAAGC AGCTGGAAGA GTTGGCACAG 
CAGGCCATAG ACCGGGCCCT GGCTGAGGGG GTGTTGCTGA GGTCCGCAAA GAACCCCAGC 
TCCTCTGACG TGGTGACGTA TGCCCCATTC ACGCTCTTCC CCTCACCAGT GCCCAGCACT 
CTGCTGGAGC AGGCCTATGC TGTGCAGATG GACTTCAACA TCCTGGTAGA TGCTGTCAGC 
CAGAACTCCG CCTTCCTGGA GCAAACACTG TCTAGCACCA TCAAAAAGGA TGAGTATACT 
GCCCGTCTCT TTGATATCTA CAAGCAAGTC CTGAAAGAGG GCATAGCCCA GACTGTGTTC 
CTGGGCCTCA ATCGTTCAGA TTACATGTTC CAGTGCAGCG CAGACGGCTC CAAAGCCCTG 
AAACAGATTG AGATCAACAC TATCTCTGCC AGCTTTGGGG GCCTGGCCTC CCGGACTCCG 
GCTGTGCACC GACATGTTCT CAATGTCCTG AATAAGACCA ACGAAGCTTC CAAGATCCTG 
TCCAACAACC CCAGCAAGGG ACTGGCCCTG GGGATCGCCA AAGCCTGGGA GCTCTATGGC 
TCAGCCAATG CCGTGGTGCT ACTGATTGCT CAGGAGAAGG AAAGGAACAT ATTTGACCAG 
CGTGCCATAG AGAACGAGCT GCTAGACAGG AAGATCCATG TAATCCGCCG AAGATTTGAA 
GATGTCTCTG AAAGGGGTTC TCTAGACCAA AACCGAAGGC TGTTTATGGA GGACCAGGAA 
GTTGCTGTGG TTTACTTCCG AGATGGCTAC ATGCCCAGTC AGTATAACGC ACAGAACTGG 
GAAGCTCGCC TGCTGCTAGA GAGATCATGT GCTGCCAAGT GTCCCGACAT TGCCACACAG 
CTGGCTGGCA CTAAGAAGGT GCAGCAGGAA CTGAGCAGGG TGGGCCTGCT GGAAGCGCTG 
CTCCCGGGCC AGCCCGAGGC TGTGGCCCGC CTCCGTGCCA CCTTTGCTGG CCTCTATTCA 
CTGGACATGG GTGAAGAAGG GGACCAGGCT GTCGCTGAGG CCCTTGCTGC CCCTAGCCAC 
TTTGTGCTGA AGCCCCAAAG AGAGGGCGGA GGTAATAACT TCTATGGGGA GGAAATGGTA 
CACGCTCTGG AGCAGCTGAA AGACAGCGAG GAGAGAGCCT CCTACATCCT CATGGAGAAG 
ATTGAACCTG AGCCTTTTAG GAATTGCTTA CTACGGCCTG GCAGCCCTGC CCAAGTGGTC 
CAGTGCATCT CGGAGCTGGG TATTTTTGGA GTCTATGTCA GACAGGGAAC AACACTTGTG 
ATGAACAAGC ATGTGGGGCA TCTGCTTCGA ACCAAAGCCA TTGAACATGC AGATGGAGGT 
GTGGCAGCAG GAGTGGCAGT CCTGGACAAC CCCTACCCTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037094.1
CDS	45..1469
Translation	MATSWGSILQDEKQLEELAQQAIDRALAEGVLLRSAKNPSSSDVVTYAPFTLFPSPVPSTLLEQAYAVQMDFNILVDAVSQNSAFLEQTLSSTIKKDEYTARLFDIYKQVLKEGIAQTVFLGLNRSDYMFQCSADGSKALKQIEINTISASFGGLASRTPAVHRHVLNVLNKTNEASKILSNNPSKGLALGIAKAWELYGSANAVVLLIAQEKERNIFDQRAIENELLDRKIHVIRRRFEDVSERGSLDQNRRLFMEDQEVAVVYFRDGYMPSQYNAQNWEARLLLERSCAAKCPDIATQLAGTKKVQQELSRVGLLEALLPGQPEAVARLRATFAGLYSLDMGEEGDQAVAEALAAPSHFVLKPQREGGGNNFYGEEMVHALEQLKDSEERASYILMEKIEPEPFRNCLLRPGSPAQVVQCISELGIFGVYVRQGTTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV

Target ORF information:

RefSeq Version	NM_012962.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione synthetase (Gss), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012962.1

ORF Insert Sequence:

ATGGCCACCA GCTGGGGAAG CATCTTGCAG GATGAGAAGC AGCTGGAAGA GTTGGCACAG 
CAGGCCATAG ACCGGGCCCT GGCTGAGGGG GTGTTGCTGA GGTCCGCAAA GAACCCCAGC 
TCCTCTGACG TGGTGACGTA TGCCCCATTC ACGCTCTTCC CCTCACCAGT GCCCAGCACT 
CTGCTGGAGC AGGCCTATGC TGTGCAGATG GACTTCAACA TCCTGGTAGA TGCTGTCAGC 
CAGAACTCCG CCTTCCTGGA GCAAACACTG TCTAGCACCA TCAAAAAGGA TGAGTATACT 
GCCCGTCTCT TTGATATCTA CAAGCAAGTC CTGAAAGAGG GCATAGCCCA GACTGTGTTC 
CTGGGCCTCA ATCGTTCAGA TTACATGTTC CAGTGCAGCG CAGACGGCTC CAAAGCCCTG 
AAACAGATTG AGATCAACAC TATCTCTGCC AGCTTTGGGG GCCTGGCCTC CCGGACTCCG 
GCTGTGCACC GACATGTTCT CAATGTCCTG AATAAGACCA ACGAAGCTTC CAAGATCCTG 
TCCAACAACC CCAGCAAGGG ACTGGCCCTG GGGATCGCCA AAGCCTGGGA GCTCTATGGC 
TCAGCCAATG CCGTGGTGCT ACTGATTGCT CAGGAGAAGG AAAGGAACAT ATTTGACCAG 
CGTGCCATAG AGAACGAGCT GCTAGACAGG AAGATCCATG TAATCCGCCG AAGATTTGAA 
GATGTCTCTG AAAGGGGTTC TCTAGACCAA AACCGAAGGC TGTTTATGGA GGACCAGGAA 
GTTGCTGTGG TTTACTTCCG AGATGGCTAC ATGCCCAGTC AGTATAACGC ACAGAACTGG 
GAAGCTCGCC TGCTGCTAGA GAGATCATGT GCTGCCAAGT GTCCCGACAT TGCCACACAG 
CTGGCTGGCA CTAAGAAGGT GCAGCAGGAA CTGAGCAGGG TGGGCCTGCT GGAAGCGCTG 
CTCCCGGGCC AGCCCGAGGC TGTGGCCCGC CTCCGTGCCA CCTTTGCTGG CCTCTATTCA 
CTGGACATGG GTGAAGAAGG GGACCAGGCT GTCGCTGAGG CCCTTGCTGC CCCTAGCCAC 
TTTGTGCTGA AGCCCCAAAG AGAGGGCGGA GGTAATAACT TCTATGGGGA GGAAATGGTA 
CACGCTCTGG AGCAGCTGAA AGACAGCGAG GAGAGAGCCT CCTACATCCT CATGGAGAAG 
ATTGAACCTG AGCCTTTTAG GAATTGCTTA CTACGGCCTG GCAGCCCTGC CCAAGTGGTC 
CAGTGCATCT CGGAGCTGGG TATTTTTGGA GTCTATGTCA GACAGGGAAC AACACTTGTG 
ATGAACAAGC ATGTGGGGCA TCTGCTTCGA ACCAAAGCCA TTGAACATGC AGATGGAGGT 
GTGGCAGCAG GAGTGGCAGT CCTGGACAAC CCCTACCCTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Toward an understanding of the protein interaction network of the human liver.
Molecular systems biology7536(2011 Oct)
Wang J,Huo K,Ma L,Tang L,Li D,Huang X,Yuan Y,Li C,Wang W,Guan W,Chen H,Jin C,Wei J,Zhang W,Yang Y,Liu Q,Zhou Y,Zhang C,Wu Z,Xu W,Zhang Y,Liu T,Yu D,Zhang Y,Chen L,Zhu D,Zhong X,Kang L,Gan X,Yu X,Ma Q,Yan J,Zhou L,Liu Z,Zhu Y,Zhou T,He F,Yang X

High fat feeding and dietary L-arginine supplementation differentially regulate gene expression in rat white adipose tissue.
Amino acids37(1)187-98(2009 May)
Jobgen W,Fu WJ,Gao H,Li P,Meininger CJ,Smith SB,Spencer TE,Wu G

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Coordinated changes in xenobiotic metabolizing enzyme gene expression in aging male rats.
Toxicological sciences : an official journal of the Society of Toxicology106(1)263-83(2008 Nov)
Lee JS,Ward WO,Wolf DC,Allen JW,Mills C,DeVito MJ,Corton JC

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Gss cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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