Cyp7b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp7b1
Entrez Gene ID	25429
Full Name	cytochrome P450 family 7 subfamily B member 1
General protein information	Preferred Names cytochrome P450 family 7 subfamily B member 1 Names 25-hydroxycholesterol 7-alpha-hydroxylase HCT-1 cytochrome P450 7B1 cytochrome P450, family 7, subfamily b, polypeptide 1 cytochrome P450, subfamily 7B, polypeptide 1 hippocampal transcript 1 protein oxysterol 7-alpha-hydroxylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q24
Summary	member of the p450 subfamily of cholesterol metabolizing enzymes that performs 7alpha-hydroxylation of 27-hydroxycholesterol in a bile synthesis pathway alternative to the classical cholesterol disposal pathway [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019138.1	NP_062011.1	25-hydroxycholesterol 7-alpha-hydroxylase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC570455	XP_699028.2
Canis lupus familiaris (dog)	CYP7B1	XP_005638074.1
Bos taurus (cattle)	CYP7B1	XP_002692741.1
Gallus gallus (chicken)	CYP7B1	XP_418276.4
Rattus norvegicus (Norway rat)	Cyp7b1	NP_062011.1
Homo sapiens (human)	CYP7B1	NP_004811.1
	LOC710188	XP_001098766.2
Xenopus tropicalis (tropical clawed frog)	cyp7b1	NP_001120511.1
Pan troglodytes (chimpanzee)	CYP7B1	XP_001159731.1
Mus musculus (house mouse)	Cyp7b1	NP_031851.3

Gene Ontology
Bibliography

Related articles in PubMed

Estrogen-mediated regulation of steroid metabolism in rat glial cells; effects on neurosteroid levels via regulation of CYP7B1-mediated catalysis.
Wicher G, Norlin M
The Journal of steroid biochemistry and molecular biology14521-7(2015 Jan)

Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.
Yi T, Wang X, Kelly LM, An J, Xu Y, Sailer AW, Gustafsson JA, Russell DW, Cyster JG
Immunity37(3)535-48(2012 Sep)

CYP7B1 mutations in French-Canadian hereditary spastic paraplegia subjects.
Noreau A, Dion PA, Szuto A, Levert A, Thibodeau P, Brais B, Dupr? N, Rioux MF, Rouleau GA
The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques39(1)91-4(2012 Jan)

[Interaction among peroxisome proliferators-activated receptor alpha, cytochrome P450 oxysterol 7?-hydroxylase and estrogen receptor and its association with intrahepatic cholestasis in pregnant rats].
Shi QY, Lin YG, Zhou X, Lin YQ, Yan S
Zhonghua fu chan ke za zhi45(6)440-4(2010 Jun)

Dramatically increased intestinal absorption of cholesterol following hypophysectomy is normalized by thyroid hormone.
G?lman C, Bonde Y, Matasconi M, Angelin B, Rudling M
Gastroenterology134(4)1127-36(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

estrogen suppresses CYP7B1-catalyzed 7alpha-hydroxylation of DEHA
Title: Estrogen-mediated regulation of steroid metabolism in rat glial cells; effects on neurosteroid levels via regulation of CYP7B1-mediated catalysis.

This study deminitrated that CYP7B1 mutations in patients with hereditary spastic paraplegia in French-Canadian.
Title: CYP7B1 mutations in French-Canadian hereditary spastic paraplegia subjects.

Abnormal expression of PPARalpha, CYP7B1 and ER may play a role in the pathogenesis of estrogen-induced intrahepatic cholestasis.
Title: [Interaction among peroxisome proliferators-activated receptor alpha, cytochrome P450 oxysterol 7?-hydroxylase and estrogen receptor and its association with intrahepatic cholestasis in pregnant rats].

Hepatic secretion of cholesterol and ABCG5/G8 expression are strongly stimulated in hypophysectomized rats during treatment with thyroid hormone.
Title: Dramatically increased intestinal absorption of cholesterol following hypophysectomy is normalized by thyroid hormone.

Kidney CYP24 activity, possibly regulated by factors such as PTH, acts in concert with kidney CYP27B1 to control serum 1,25D levels.
Title: Quantification of mRNA for the vitamin D metabolizing enzymes CYP27B1 and CYP24 and vitamin D receptor in kidney using real-time reverse transcriptase- polymerase chain reaction.

The following Cyp7b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp7b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03827	NM_019138.1 Latest version!	Rattus norvegicus cytochrome P450 family 7 subfamily B member 1 (Cyp7b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp7b1 ( NM_019138.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062011.1 Rattus norvegicus cytochrome P450 family 7 subfamily B member 1 (Cyp7b1), mRNA.

Price & Availability↑

CloneID	ORa03827
Clone ID Related Accession (Same CDS sequence)	NM_019138.1
Accession Version	NM_019138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	25-hydroxycholesterol 7-alpha-hydroxylase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB800565.1, CB760136.1, U36992.1, BF283070.1 and CB749347.1. On or before Oct 13, 2007 this sequence version replaced XM_001060253.1, XM_342218.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGAG CCACGACCCC AGATGCCGCT TCGCCCGGGC CTCTCTCCCT ACTAGGCCTT 
CTCTTTGCGG TCACCTTGCT GCTCCCAGTC CTGTTCCTCC TCACCCGGCG CACAAGGCGT 
CCTTGTGAAC CTCCCTTGAT AAAAGGTTGG ATTCCTTATC TTGGCATGGC CCTGAAATTC 
TGGAAGGATC CGTTAGCTTT CTTGCAAACT CTTCAAAGGC AATGTGGTGA CACTTTCACT 
GTCTTACTTG GAGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ATGAAAAACC CAAAACAATT AAGCTTTGAG AAGTTCAGCC GAAGATTATC AGCGAAAGCC 
TTCTCTGTCA AGAAGCTGCT AACTAATGAC GACCTTAGCA ATGACATTCA CAGAGGCTAT 
CTTCTTTTAC AAGGCAAATC TCTGGATGGT CTTCTGGAAA CCATGATCCA AGAAGTAAAA 
GAAATATTTG AGTCCAGACT GCTAAAACTC ACAGATTGGA ATACAGCAAG AGTATTTGAT 
TTCTGTAGTT CACTGGTATT TGAAATCACA TTTACAACTA TATATGGAAA AATTCTTGCT 
GCTAACAAAA AACAAATTAT CAGTGAGCTG AGGGATGATT TTTTAAAATT TGATGACCAT 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGCAGA ATTTATGCAG 
AAGAAAATTA TAAAATGTCT CACACCAGAA AAAGTAGCTC AGATGCAAAG ACGGTCAGAA 
ATTGTTCAGG AGAGGCAGGA GATGCTGAAA AAATACTACG GGCATGAAGA GTTTGAAATA 
GGAGCACATC ATCTTGGCTT GCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATCTTCT TCAGCATCCA GAAGCTATGG AAGTCCTGCG TGACGAAATT 
GACAGCTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCTGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GAAAGCGCTA TTCTTGAGGT TCTGAGGTTG 
TGCTCCTACT CCAGCATCAT CCGTGAAGTG CAAGAGGATA TGGATTTCAG CTCAGAGAGT 
AGGAGCTACC GTCTGCGGAA AGGAGACTTT GTAGCTGTCT TTCCTCCAAT GATACACAAT 
GACCCAGAAG TCTTCGATGC TCCAAAGGAC TTTAGGTTTG ATCGCTTCGT AGAAGATGGT 
AAGAAGAAAA CAACGTTTTT CAAAGGAGGA AAAAAGCTGA AGAGTTACAT TATACCATTT 
GGACTTGGAA CAAGCAAATG TCCAGGCAGA TACTTTGCAA TTAATGAAAT GAAGCTACTA 
GTGATTATAC TTTTAACTTA TTTTGATTTA GAAGTCATTG ACACTAAGCC TATAGGACTA 
AACCACAGTC GCATGTTTCT GGGCATTCAG CATCCAGACT CTGACATCTC ATTTAGGTAC 
AAGGCAAAAT CTTGGAGATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062011.1
CDS	119..1642
Translation	MEGATTPDAASPGPLSLLGLLFAVTLLLPVLFLLTRRTRRPCEPPLIKGWIPYLGMALKFWKDPLAFLQTLQRQCGDTFTVLLGGKYITFVLNPFQYQYVMKNPKQLSFEKFSRRLSAKAFSVKKLLTNDDLSNDIHRGYLLLQGKSLDGLLETMIQEVKEIFESRLLKLTDWNTARVFDFCSSLVFEITFTTIYGKILAANKKQIISELRDDFLKFDDHFPYLVSDIPIQLLRNAEFMQKKIIKCLTPEKVAQMQRRSEIVQERQEMLKKYYGHEEFEIGAHHLGLLWASLANTIPAMFWAMYYLLQHPEAMEVLRDEIDSFLQSTGQKKGPGISVHFTREQLDSLVCLESAILEVLRLCSYSSIIREVQEDMDFSSESRSYRLRKGDFVAVFPPMIHNDPEVFDAPKDFRFDRFVEDGKKKTTFFKGGKKLKSYIIPFGLGTSKCPGRYFAINEMKLLVIILLTYFDLEVIDTKPIGLNHSRMFLGIQHPDSDISFRYKAKSWRS

Target ORF information:

RefSeq Version	NM_019138.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450 family 7 subfamily B member 1 (Cyp7b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019138.1

ORF Insert Sequence:

ATGGAGGGAG CCACGACCCC AGATGCCGCT TCGCCCGGGC CTCTCTCCCT ACTAGGCCTT 
CTCTTTGCGG TCACCTTGCT GCTCCCAGTC CTGTTCCTCC TCACCCGGCG CACAAGGCGT 
CCTTGTGAAC CTCCCTTGAT AAAAGGTTGG ATTCCTTATC TTGGCATGGC CCTGAAATTC 
TGGAAGGATC CGTTAGCTTT CTTGCAAACT CTTCAAAGGC AATGTGGTGA CACTTTCACT 
GTCTTACTTG GAGGGAAGTA TATAACATTT GTTCTGAACC CTTTCCAGTA CCAGTATGTA 
ATGAAAAACC CAAAACAATT AAGCTTTGAG AAGTTCAGCC GAAGATTATC AGCGAAAGCC 
TTCTCTGTCA AGAAGCTGCT AACTAATGAC GACCTTAGCA ATGACATTCA CAGAGGCTAT 
CTTCTTTTAC AAGGCAAATC TCTGGATGGT CTTCTGGAAA CCATGATCCA AGAAGTAAAA 
GAAATATTTG AGTCCAGACT GCTAAAACTC ACAGATTGGA ATACAGCAAG AGTATTTGAT 
TTCTGTAGTT CACTGGTATT TGAAATCACA TTTACAACTA TATATGGAAA AATTCTTGCT 
GCTAACAAAA AACAAATTAT CAGTGAGCTG AGGGATGATT TTTTAAAATT TGATGACCAT 
TTCCCATACT TAGTATCTGA CATACCTATT CAGCTTCTAA GAAATGCAGA ATTTATGCAG 
AAGAAAATTA TAAAATGTCT CACACCAGAA AAAGTAGCTC AGATGCAAAG ACGGTCAGAA 
ATTGTTCAGG AGAGGCAGGA GATGCTGAAA AAATACTACG GGCATGAAGA GTTTGAAATA 
GGAGCACATC ATCTTGGCTT GCTCTGGGCC TCTCTAGCAA ACACCATTCC AGCTATGTTC 
TGGGCAATGT ATTATCTTCT TCAGCATCCA GAAGCTATGG AAGTCCTGCG TGACGAAATT 
GACAGCTTCC TGCAGTCAAC AGGTCAAAAG AAAGGACCTG GAATTTCTGT CCACTTCACC 
AGAGAACAAT TGGACAGCTT GGTCTGCCTG GAAAGCGCTA TTCTTGAGGT TCTGAGGTTG 
TGCTCCTACT CCAGCATCAT CCGTGAAGTG CAAGAGGATA TGGATTTCAG CTCAGAGAGT 
AGGAGCTACC GTCTGCGGAA AGGAGACTTT GTAGCTGTCT TTCCTCCAAT GATACACAAT 
GACCCAGAAG TCTTCGATGC TCCAAAGGAC TTTAGGTTTG ATCGCTTCGT AGAAGATGGT 
AAGAAGAAAA CAACGTTTTT CAAAGGAGGA AAAAAGCTGA AGAGTTACAT TATACCATTT 
GGACTTGGAA CAAGCAAATG TCCAGGCAGA TACTTTGCAA TTAATGAAAT GAAGCTACTA 
GTGATTATAC TTTTAACTTA TTTTGATTTA GAAGTCATTG ACACTAAGCC TATAGGACTA 
AACCACAGTC GCATGTTTCT GGGCATTCAG CATCCAGACT CTGACATCTC ATTTAGGTAC 
AAGGCAAAAT CTTGGAGATC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Estrogen-mediated regulation of steroid metabolism in rat glial cells; effects on neurosteroid levels via regulation of CYP7B1-mediated catalysis.
The Journal of steroid biochemistry and molecular biology14521-7(2015 Jan)
Wicher G,Norlin M

Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.
Immunity37(3)535-48(2012 Sep)
Yi T,Wang X,Kelly LM,An J,Xu Y,Sailer AW,Gustafsson JA,Russell DW,Cyster JG

CYP7B1 mutations in French-Canadian hereditary spastic paraplegia subjects.
The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques39(1)91-4(2012 Jan)
Noreau A,Dion PA,Szuto A,Levert A,Thibodeau P,Brais B,Dupr? N,Rioux MF,Rouleau GA

[Interaction among peroxisome proliferators-activated receptor alpha, cytochrome P450 oxysterol 7?-hydroxylase and estrogen receptor and its association with intrahepatic cholestasis in pregnant rats].
Zhonghua fu chan ke za zhi45(6)440-4(2010 Jun)
Shi QY,Lin YG,Zhou X,Lin YQ,Yan S

Dramatically increased intestinal absorption of cholesterol following hypophysectomy is normalized by thyroid hormone.
Gastroenterology134(4)1127-36(2008 Apr)
G?lman C,Bonde Y,Matasconi M,Angelin B,Rudling M

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Cyp7b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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