Cpt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cpt2
Entrez Gene ID	25413
Full Name	carnitine palmitoyltransferase 2
Synonyms	CPTII
General protein information	Preferred Names carnitine palmitoyltransferase 2 Names carnitine O-palmitoyltransferase 2, mitochondrial CPT II carnitine palmitoyltransferase II
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q34
Summary	inner mitochondrial membrane protein that converts acylcarnitine to acyl-CoA [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012930.1	NP_037062.1	carnitine O-palmitoyltransferase 2, mitochondrial precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CPT2	NP_001039354.1
Caenorhabditis elegans (roundworm)	cpt-2	NP_001040977.1
Saccharomyces cerevisiae (baker's yeast)	YAT1	NP_009420.1
Eremothecium gossypii	AGOS_AER224W	NP_985081.1
Magnaporthe oryzae (rice blast fungus)	MGG_06981	XP_003709730.1
	CPT2	XP_001112489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008907	XP_552786.2
Homo sapiens (human)	CPT2	NP_000089.1
Pan troglodytes (chimpanzee)	CPT2	XP_001148628.1
Gallus gallus (chicken)	CPT2	NP_001026458.2
Danio rerio (zebrafish)	cpt2	NP_001007448.1
Neurospora crassa	NCU01611	XP_956522.1
Rattus norvegicus (Norway rat)	Cpt2	NP_037062.1
Drosophila melanogaster (fruit fly)	CG2107	NP_647756.1
Xenopus tropicalis (tropical clawed frog)	cpt2	NP_989193.1
Canis lupus familiaris (dog)	CPT2	XP_546705.1
Mus musculus (house mouse)	Cpt2	NP_034079.2
Kluyveromyces lactis	KLLA0C04169g	XP_452383.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial carnitine palmitoyl transferase-II inactivity aggravates lipid accumulation in rat hepatocarcinogenesis.
Gu JJ, Yao M, Yang J, Cai Y, Zheng WJ, Wang L, Yao DB, Yao DF
World journal of gastroenterology23(2)256-264(2017 Jan)

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wilson R, Geyer SH, Reissig L, Rose J, Szumska D, Hardman E, Prin F, McGuire C, Ramirez-Solis R, White J, Galli A, Tudor C, Tuck E, Mazzeo CI, Smith JC, Robertson E, Adams DJ, Mohun T, Weninger WJ
Wellcome open research11(2016)

Adipose fatty acid oxidation is required for thermogenesis and potentiates oxidative stress-induced inflammation.
Lee J, Ellis JM, Wolfgang MJ
Cell reports10(2)266-79(2015 Jan)

Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.
Console L, Giangregorio N, Indiveri C, Tonazzi A
Molecular and cellular biochemistry394(1-2)307-14(2014 Sep)

Structural insight into function and regulation of carnitine palmitoyltransferase.
Rufer AC, Thoma R, Hennig M
Cellular and molecular life sciences : CMLS66(15)2489-501(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Low CPT-II expression might lead to abnormal hepatic lipid accumulation, which should promote the malignant transformation of hepatocytes.
Title: Mitochondrial carnitine palmitoyl transferase-II inactivity aggravates lipid accumulation in rat hepatocarcinogenesis.

Report interaction between carnitine palmitoyltransferase 2 and carnitine/acylcarnitine translocase supporting the channeling of acylcarnitines and carnitine at level of the inner mitochondrial membrane.
Title: Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.

The protein interacts with the membrane as a functional monomer and the calculations confirm the presence of a membrane association domain that consists of layers of hydrophobic and positively charged residues.
Title: Carnitine palmitoyltransferase 2: analysis of membrane association and complex structure with a substrate analog.

surface patch mediates the association of CPT-II with the inner membrane of the mitochondria
Title: Crystal structure of rat carnitine palmitoyltransferase II (CPT-II).

crystal structure of carnitine palmitoyltransferase 2
Title: The crystal structure of carnitine palmitoyltransferase 2 and implications for diabetes treatment.

The following Cpt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cpt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12499	NM_012930.1 Latest version!	Rattus norvegicus carnitine palmitoyltransferase 2 (Cpt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cpt2 ( NM_012930.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037062.1 Rattus norvegicus carnitine palmitoyltransferase 2 (Cpt2), mRNA.

Price & Availability↑

CloneID	ORa12499
Clone ID Related Accession (Same CDS sequence)	NM_012930.1
Accession Version	NM_012930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-palmitoyltransferase 2, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05470.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05470.1, U88295.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATGCCGC GCCTGCTGTT TCGTGCCTGG CCCCGCTGCC CCTCGCTTGT CCTGGGAGCC 
CCTAGTAGGC CCTTAAGTGC TGTCTCGGGG CCGGACGATT ATCTGCAGCA CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TCCCTAAACT TGAAGACACC 
ATGAAGAGAT ACCTCAATGC ACAGAAGCCT CTCTTGGATG ACAGCCAGTT CAGGAGAACA 
GAAGCGTTGT GTAAGAATTT TGAGACTGGC GTTGGGAAGG AGCTGCATGC CCACCTGCTT 
GCTCAGGATA AGCAGAATAA GCACACCAGC TACATCTCAG GCCCCTGGTT CGATATGTAT 
TTAACTGCTC GAGACTCTAT CGTTTTAAAC TTTAATCCAT TTATGGCATT CAATCCGGAC 
CCAAAGTCTG AGTATAATGA CCAGCTTACC AGGGCAACCA ACTTGACTGT TTCTGCAGTC 
CGGTTTCTGA AGACACTTCA GGCTGGCCTT TTAGAACCAG AAGTGTTCCA CTTGAACCCT 
TCCAAAAGTG ACACAGACGC CTTCAAAAGA CTCATTCGCT TTGTTCCTCC CTCTCTGTCC 
TGGTATGGAG CCTACCTGGT CAACGCATAT CCCCTGGATA TGTCCCAGTA TTTCCGGCTT 
TTCAATTCAA CTCGTATACC CAGACCCAAT CGTGATGAAC TCTTTACTGA CACCAAGGCT 
AGACACCTCC TGGTCCTAAG AAAAGGACAT TTCTATGTCT TTGATGTCCT CGATCAAGAT 
GGGAACATTG TGAATCCCTT AGAGATTCAG GCACATCTAA AGTACATTCT CTCAGACAGC 
AGCCCTGTGC CCGAGTTTCC TGTGGCATAT CTGACCAGTG AGAACCGAGA TGTCTGGGCA 
GAGCTCAGAC AGAAGTTGAT TTTTGATGGC AATGAGGAAA CCCTGAAGAA AGTGGACTCT 
GCTGTCTTCT GCCTCTGCCT AGATGACTTC CCGATGAAGG ATCTTATTCA CTTGTCACAC 
ACCATGCTGC ACGGCGATGG CACAAACCGC TGGTTTGATA AGTCCTTTAA CCTCATTGTA 
GCCGAGGACG GCACTGCTGC AGTCCACTTC GAGCACTCGT GGGGGGATGG GGTGGCAGTG 
CTTAGGTTTT TCAATGAAGT GTTCAGAGAC AGCACTCAGA CCCCTGCCAT CACTCCCCAG 
AGCCAGCCTG CCGCCACCAA CTCCTCTGCG TCTGTGGAGA CGCTCAGCTT CAACCTCAGC 
GGTGCTCTCA AGGCTGGCAT CACTGCTGCC AAGGAGAAGT TCGACACCAC GGTGAAAACC 
CTCAGCATTG ACTCCATTCA GTTCCAGAGA GGTGGCAAGG AGTTCCTGAA GAAGAAGCAG 
CTGAGCCCCG ATGCGGTGGC CCAGCTGGCC TTCCAGATGG CCTTCCTGAG ACAGTATGGC 
CAGACGGTGG CTACCTATGA GTCCTGCAGC ACTGCAGCCT TCAAGCACGG CCGCACAGAG 
ACTATCCGCC CAGCCTCCAT CTTTACTAAG AGATGCTCCG AGGCGTTTGT CAGGGACCCC 
TCCAAGCACA GTGTGGGCGA GCTTCAGCAC ATGATGGCTG AGTGTTCCAA ATACCATGGC 
CAGCTGACCA AAGAAGCAGC GATGGGCCAG GGCTTTGACC GACACTTGTA TGCTCTGCGC 
TATCTGGCAA CGGCCAGAGG ACTCAACCTA CCTGAGCTCT ATCTGGATCC TGCATACCAG 
CAGATGAACC ACAACATCCT GTCCACCAGC ACTCTGAACA GCCCAGCAGT GAGCCTTGGG 
GGCTTTGCCC CTGTGGTCCC TGATGGCTTT GGCATTGCAT ATGCTGTTCA CGATGACTGG 
ATAGGCTGCA ATGTCTCCTC CTACTCAGGA CGCAATGCCC GAGAGTTTCT CCACTGTGTC 
CAGAAGTGCT TGGAAGACAT TTTCGATGCT CTAGAAGGCA AAGCCATCAA AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037062.1
CDS	63..2039
Translation	MMPRLLFRAWPRCPSLVLGAPSRPLSAVSGPDDYLQHSIVPTMHYQDSLPRLPIPKLEDTMKRYLNAQKPLLDDSQFRRTEALCKNFETGVGKELHAHLLAQDKQNKHTSYISGPWFDMYLTARDSIVLNFNPFMAFNPDPKSEYNDQLTRATNLTVSAVRFLKTLQAGLLEPEVFHLNPSKSDTDAFKRLIRFVPPSLSWYGAYLVNAYPLDMSQYFRLFNSTRIPRPNRDELFTDTKARHLLVLRKGHFYVFDVLDQDGNIVNPLEIQAHLKYILSDSSPVPEFPVAYLTSENRDVWAELRQKLIFDGNEETLKKVDSAVFCLCLDDFPMKDLIHLSHTMLHGDGTNRWFDKSFNLIVAEDGTAAVHFEHSWGDGVAVLRFFNEVFRDSTQTPAITPQSQPAATNSSASVETLSFNLSGALKAGITAAKEKFDTTVKTLSIDSIQFQRGGKEFLKKKQLSPDAVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASIFTKRCSEAFVRDPSKHSVGELQHMMAECSKYHGQLTKEAAMGQGFDRHLYALRYLATARGLNLPELYLDPAYQQMNHNILSTSTLNSPAVSLGGFAPVVPDGFGIAYAVHDDWIGCNVSSYSGRNAREFLHCVQKCLEDIFDALEGKAIKT

Target ORF information:

RefSeq Version	NM_012930.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine palmitoyltransferase 2 (Cpt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012930.1

ORF Insert Sequence:

ATGATGCCGC GCCTGCTGTT TCGTGCCTGG CCCCGCTGCC CCTCGCTTGT CCTGGGAGCC 
CCTAGTAGGC CCTTAAGTGC TGTCTCGGGG CCGGACGATT ATCTGCAGCA CAGCATCGTG 
CCCACCATGC ACTACCAGGA CAGCCTGCCC AGGCTGCCTA TCCCTAAACT TGAAGACACC 
ATGAAGAGAT ACCTCAATGC ACAGAAGCCT CTCTTGGATG ACAGCCAGTT CAGGAGAACA 
GAAGCGTTGT GTAAGAATTT TGAGACTGGC GTTGGGAAGG AGCTGCATGC CCACCTGCTT 
GCTCAGGATA AGCAGAATAA GCACACCAGC TACATCTCAG GCCCCTGGTT CGATATGTAT 
TTAACTGCTC GAGACTCTAT CGTTTTAAAC TTTAATCCAT TTATGGCATT CAATCCGGAC 
CCAAAGTCTG AGTATAATGA CCAGCTTACC AGGGCAACCA ACTTGACTGT TTCTGCAGTC 
CGGTTTCTGA AGACACTTCA GGCTGGCCTT TTAGAACCAG AAGTGTTCCA CTTGAACCCT 
TCCAAAAGTG ACACAGACGC CTTCAAAAGA CTCATTCGCT TTGTTCCTCC CTCTCTGTCC 
TGGTATGGAG CCTACCTGGT CAACGCATAT CCCCTGGATA TGTCCCAGTA TTTCCGGCTT 
TTCAATTCAA CTCGTATACC CAGACCCAAT CGTGATGAAC TCTTTACTGA CACCAAGGCT 
AGACACCTCC TGGTCCTAAG AAAAGGACAT TTCTATGTCT TTGATGTCCT CGATCAAGAT 
GGGAACATTG TGAATCCCTT AGAGATTCAG GCACATCTAA AGTACATTCT CTCAGACAGC 
AGCCCTGTGC CCGAGTTTCC TGTGGCATAT CTGACCAGTG AGAACCGAGA TGTCTGGGCA 
GAGCTCAGAC AGAAGTTGAT TTTTGATGGC AATGAGGAAA CCCTGAAGAA AGTGGACTCT 
GCTGTCTTCT GCCTCTGCCT AGATGACTTC CCGATGAAGG ATCTTATTCA CTTGTCACAC 
ACCATGCTGC ACGGCGATGG CACAAACCGC TGGTTTGATA AGTCCTTTAA CCTCATTGTA 
GCCGAGGACG GCACTGCTGC AGTCCACTTC GAGCACTCGT GGGGGGATGG GGTGGCAGTG 
CTTAGGTTTT TCAATGAAGT GTTCAGAGAC AGCACTCAGA CCCCTGCCAT CACTCCCCAG 
AGCCAGCCTG CCGCCACCAA CTCCTCTGCG TCTGTGGAGA CGCTCAGCTT CAACCTCAGC 
GGTGCTCTCA AGGCTGGCAT CACTGCTGCC AAGGAGAAGT TCGACACCAC GGTGAAAACC 
CTCAGCATTG ACTCCATTCA GTTCCAGAGA GGTGGCAAGG AGTTCCTGAA GAAGAAGCAG 
CTGAGCCCCG ATGCGGTGGC CCAGCTGGCC TTCCAGATGG CCTTCCTGAG ACAGTATGGC 
CAGACGGTGG CTACCTATGA GTCCTGCAGC ACTGCAGCCT TCAAGCACGG CCGCACAGAG 
ACTATCCGCC CAGCCTCCAT CTTTACTAAG AGATGCTCCG AGGCGTTTGT CAGGGACCCC 
TCCAAGCACA GTGTGGGCGA GCTTCAGCAC ATGATGGCTG AGTGTTCCAA ATACCATGGC 
CAGCTGACCA AAGAAGCAGC GATGGGCCAG GGCTTTGACC GACACTTGTA TGCTCTGCGC 
TATCTGGCAA CGGCCAGAGG ACTCAACCTA CCTGAGCTCT ATCTGGATCC TGCATACCAG 
CAGATGAACC ACAACATCCT GTCCACCAGC ACTCTGAACA GCCCAGCAGT GAGCCTTGGG 
GGCTTTGCCC CTGTGGTCCC TGATGGCTTT GGCATTGCAT ATGCTGTTCA CGATGACTGG 
ATAGGCTGCA ATGTCTCCTC CTACTCAGGA CGCAATGCCC GAGAGTTTCT CCACTGTGTC 
CAGAAGTGCT TGGAAGACAT TTTCGATGCT CTAGAAGGCA AAGCCATCAA AACTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial carnitine palmitoyl transferase-II inactivity aggravates lipid accumulation in rat hepatocarcinogenesis.
World journal of gastroenterology23(2)256-264(2017 Jan)
Gu JJ,Yao M,Yang J,Cai Y,Zheng WJ,Wang L,Yao DB,Yao DF

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wellcome open research11(2016)
Wilson R,Geyer SH,Reissig L,Rose J,Szumska D,Hardman E,Prin F,McGuire C,Ramirez-Solis R,White J,Galli A,Tudor C,Tuck E,Mazzeo CI,Smith JC,Robertson E,Adams DJ,Mohun T,Weninger WJ

Adipose fatty acid oxidation is required for thermogenesis and potentiates oxidative stress-induced inflammation.
Cell reports10(2)266-79(2015 Jan)
Lee J,Ellis JM,Wolfgang MJ

Carnitine/acylcarnitine translocase and carnitine palmitoyltransferase 2 form a complex in the inner mitochondrial membrane.
Molecular and cellular biochemistry394(1-2)307-14(2014 Sep)
Console L,Giangregorio N,Indiveri C,Tonazzi A

Structural insight into function and regulation of carnitine palmitoyltransferase.
Cellular and molecular life sciences : CMLS66(15)2489-501(2009 Aug)
Rufer AC,Thoma R,Hennig M

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Cpt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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