Ambn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ambn
Entrez Gene ID	25376
Full Name	ameloblastin
General protein information	Preferred Names ameloblastin Names ameloblastin amelin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	may act as a structural component of tooth enamel matrix [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012900.1	NP_037032.1	ameloblastin precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ambn	NP_033794.1
	AMBN	XP_001105992.1
Homo sapiens (human)	AMBN	NP_057603.1
Canis lupus familiaris (dog)	AMBN	XP_539304.3
Rattus norvegicus (Norway rat)	Ambn	NP_037032.1
Bos taurus (cattle)	AMBN	NP_776413.1
Pan troglodytes (chimpanzee)	AMBN	XP_001160377.1
Xenopus tropicalis (tropical clawed frog)	ambn	XP_002938667.2

Gene Ontology
Bibliography

Related articles in PubMed

High-Dose Fluoride Induces Apoptosis and Inhibits Ameloblastin Secretion in Primary Rat Ameloblast.
Wang L, Zhu Y, Wang D
Biological trace element research174(2)402-409(2016 Dec)

Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair.
Tamburstuen MV, Reseland JE, Spahr A, Brookes SJ, Kvalheim G, Slaby I, Snead ML, Lyngstadaas SP
Bone48(2)406-13(2011 Feb)

Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.
Iizuka S, Kudo Y, Yoshida M, Tsunematsu T, Yoshiko Y, Uchida T, Ogawa I, Miyauchi M, Takata T
Molecular and cellular biology31(4)783-92(2011 Feb)

Critical role of heparin binding domains of ameloblastin for dental epithelium cell adhesion and ameloblastoma proliferation.
Sonoda A, Iwamoto T, Nakamura T, Fukumoto E, Yoshizaki K, Yamada A, Arakaki M, Harada H, Nonaka K, Nakamura S, Yamada Y, Fukumoto S
The Journal of biological chemistry284(40)27176-84(2009 Oct)

Ameloblastin and amelogenin share a common secretory pathway and are co-secreted during enamel formation.
Zalzal SF, Smith CE, Nanci A
Matrix biology : journal of the International Society for Matrix Biology27(4)352-9(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fluoride could decrease ameloblast viability, induce ameloblast apoptosis via activating FasL/Fas signaling pathway, and reduce AMBN secretion.
Title: High-Dose Fluoride Induces Apoptosis and Inhibits Ameloblastin Secretion in Primary Rat Ameloblast.

Findings suggest a role for this protein in early bone formation and repair.
Title: Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair.

ameloblastin is expressed in osteoblasts and functions as a promoting factor for osteogenic differentiation via a novel pathway through the interaction between CD63 and integrin beta1
Title: Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.

AMBN promotes cell binding through the heparin binding sites and plays an important role in preventing odontogenic tumor development by suppressing cell proliferation and maintaining differentiation phenotype through Msx2, p21, and p27.
Title: Critical role of heparin binding domains of ameloblastin for dental epithelium cell adhesion and ameloblastoma proliferation.

subpopulation of granules containing only AMBN could be responsible for augmenting its local concentration along secretory surfaces against which hydroxyapatite crystals actively elongate
Title: Ameloblastin and amelogenin share a common secretory pathway and are co-secreted during enamel formation.

The following Ambn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ambn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42500	NM_012900.1 Latest version!	Rattus norvegicus ameloblastin (Ambn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ambn ( NM_012900.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037032.1 Rattus norvegicus ameloblastin (Ambn), mRNA.

Price & Availability↑

CloneID	ORa42500
Clone ID Related Accession (Same CDS sequence)	NM_012900.1
Accession Version	NM_012900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Rattus norvegicus(Norway rat)
Product	ameloblastin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U35097.1. On Jun 25, 2006 this sequence version replaced XM_001073587.1. ##Evidence-Data-START## Transcript exon combination :: U35097.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN02642615, SAMN02642676 [ECO:0000350] ##Evidence-Data-END##

ATGTCAGCAT CTAAGATTCC ACTTTTCAAA ATGAAGGGCC TGCTCCTGTT CCTGTCCCTA 
GTGAAAATGA GCCTCGCCGT GCCGGCATTT CCTCAACAAC CTGGGGCTCA AGGCATGGCA 
CCTCCTGGCA TGGCTAGTTT GAGCCTTGAG ACAATGAGAC AGTTGGGAAG CTTGCAGGGG 
CTCAACGCAC TTTCTCAGTA TTCTAGACTT GGCTTTGGAA AAGCACTTAA TAGTTTATGG 
TTGCATGGAC TCCTCCCACC GCATAATTCT TTCCCATGGA TAGGACCAAG GGAACATGAA 
ACCCAACAGT ATGAATATTC TTTGCCTGTG CATCCCCCAC CTCTCCCATC ACAGCCATCC 
TTGCAGCCTC ACCAGCCAGG ACTGAAACCC TTCCTCCAGC CCACTGCTGC AACCGGTGTC 
CAGGTCACAC CCCAGAAGCC AGGGCCTCAT CCTCCAATGC ACCCTGGACA GCTGCCCTTG 
CAGGAAGGAG AGCTGATAGC ACCAGATGAG CCACAGGTGG CGCCATCAGA GAACCCACCA 
ACACCCGAGG TACCAATAAT GGATTTTGCC GATCCACAAT TCCCAACAGT GTTCCAGATC 
GCCCATTCGC TGTCTCGGGG ACCAATGGCA CACAACAAAG TACCCACTTT TTACCCAGGA 
ATGTTTTACA TGTCTTATGG AGCAAACCAA TTGAATGCTC CTGCCAGAAT CGGCTTCATG 
AGTTCAGAAG AAATGCCTGG AGAAAGAGGA AGTCCCATGG CCTACGGAAC TCTGTTCCCA 
GGATATGGAG GCTTCAGGCA AACCCTTAGG GGACTGAATC AGAATTCACC CAAGGGAGGA 
GACTTTACTG TGGAAGTAGA TTCTCCAGTG TCTGTAACTA AAGGCCCTGA GAAAGGAGAG 
GGTCCAGAAG GCTCTCCACT GCAAGAGGCC AGCCCAGACA AGGGCGAAAA CCCGGCTCTC 
CTTTCACAGA TGGCCCCCGG GGCCCATGCA GGACTTCTTG CTTTCCCCAA TGACCACATC 
CCCAACATGG CAAGGGGTCC TGCAGGGCAA AGACTCCTCG GAGTCACCCC TGCAGCTGCA 
GACCCACTGA TCACCCCTGA ATTAGCAGAA GTTTATGAAA CCTATGGTGC TGATGTTACC 
ACACCCTTGG GGGATGGAGA AGCAACCATG GATATCACCA TGTCCCCAGA CACTCAGCAG 
CCACCGATGC CTGGAAACAA AGTGCACCAG CCCCAGGTGC ACAATGCATG GCGTTTCCAA 
GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037032.1
CDS	91..1359
Translation	MSASKIPLFKMKGLLLFLSLVKMSLAVPAFPQQPGAQGMAPPGMASLSLETMRQLGSLQGLNALSQYSRLGFGKALNSLWLHGLLPPHNSFPWIGPREHETQQYEYSLPVHPPPLPSQPSLQPHQPGLKPFLQPTAATGVQVTPQKPGPHPPMHPGQLPLQEGELIAPDEPQVAPSENPPTPEVPIMDFADPQFPTVFQIAHSLSRGPMAHNKVPTFYPGMFYMSYGANQLNAPARIGFMSSEEMPGERGSPMAYGTLFPGYGGFRQTLRGLNQNSPKGGDFTVEVDSPVSVTKGPEKGEGPEGSPLQEASPDKGENPALLSQMAPGAHAGLLAFPNDHIPNMARGPAGQRLLGVTPAAADPLITPELAEVYETYGADVTTPLGDGEATMDITMSPDTQQPPMPGNKVHQPQVHNAWRFQEP

Target ORF information:

RefSeq Version	NM_012900.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ameloblastin (Ambn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012900.1

ORF Insert Sequence:

ATGTCAGCAT CTAAGATTCC ACTTTTCAAA ATGAAGGGCC TGCTCCTGTT CCTGTCCCTA 
GTGAAAATGA GCCTCGCCGT GCCGGCATTT CCTCAACAAC CTGGGGCTCA AGGCATGGCA 
CCTCCTGGCA TGGCTAGTTT GAGCCTTGAG ACAATGAGAC AGTTGGGAAG CTTGCAGGGG 
CTCAACGCAC TTTCTCAGTA TTCTAGACTT GGCTTTGGAA AAGCACTTAA TAGTTTATGG 
TTGCATGGAC TCCTCCCACC GCATAATTCT TTCCCATGGA TAGGACCAAG GGAACATGAA 
ACCCAACAGT ATGAATATTC TTTGCCTGTG CATCCCCCAC CTCTCCCATC ACAGCCATCC 
TTGCAGCCTC ACCAGCCAGG ACTGAAACCC TTCCTCCAGC CCACTGCTGC AACCGGTGTC 
CAGGTCACAC CCCAGAAGCC AGGGCCTCAT CCTCCAATGC ACCCTGGACA GCTGCCCTTG 
CAGGAAGGAG AGCTGATAGC ACCAGATGAG CCACAGGTGG CGCCATCAGA GAACCCACCA 
ACACCCGAGG TACCAATAAT GGATTTTGCC GATCCACAAT TCCCAACAGT GTTCCAGATC 
GCCCATTCGC TGTCTCGGGG ACCAATGGCA CACAACAAAG TACCCACTTT TTACCCAGGA 
ATGTTTTACA TGTCTTATGG AGCAAACCAA TTGAATGCTC CTGCCAGAAT CGGCTTCATG 
AGTTCAGAAG AAATGCCTGG AGAAAGAGGA AGTCCCATGG CCTACGGAAC TCTGTTCCCA 
GGATATGGAG GCTTCAGGCA AACCCTTAGG GGACTGAATC AGAATTCACC CAAGGGAGGA 
GACTTTACTG TGGAAGTAGA TTCTCCAGTG TCTGTAACTA AAGGCCCTGA GAAAGGAGAG 
GGTCCAGAAG GCTCTCCACT GCAAGAGGCC AGCCCAGACA AGGGCGAAAA CCCGGCTCTC 
CTTTCACAGA TGGCCCCCGG GGCCCATGCA GGACTTCTTG CTTTCCCCAA TGACCACATC 
CCCAACATGG CAAGGGGTCC TGCAGGGCAA AGACTCCTCG GAGTCACCCC TGCAGCTGCA 
GACCCACTGA TCACCCCTGA ATTAGCAGAA GTTTATGAAA CCTATGGTGC TGATGTTACC 
ACACCCTTGG GGGATGGAGA AGCAACCATG GATATCACCA TGTCCCCAGA CACTCAGCAG 
CCACCGATGC CTGGAAACAA AGTGCACCAG CCCCAGGTGC ACAATGCATG GCGTTTCCAA 
GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-Dose Fluoride Induces Apoptosis and Inhibits Ameloblastin Secretion in Primary Rat Ameloblast.
Biological trace element research174(2)402-409(2016 Dec)
Wang L,Zhu Y,Wang D

Ameloblastin expression and putative autoregulation in mesenchymal cells suggest a role in early bone formation and repair.
Bone48(2)406-13(2011 Feb)
Tamburstuen MV,Reseland JE,Spahr A,Brookes SJ,Kvalheim G,Slaby I,Snead ML,Lyngstadaas SP

Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.
Molecular and cellular biology31(4)783-92(2011 Feb)
Iizuka S,Kudo Y,Yoshida M,Tsunematsu T,Yoshiko Y,Uchida T,Ogawa I,Miyauchi M,Takata T

Critical role of heparin binding domains of ameloblastin for dental epithelium cell adhesion and ameloblastoma proliferation.
The Journal of biological chemistry284(40)27176-84(2009 Oct)
Sonoda A,Iwamoto T,Nakamura T,Fukumoto E,Yoshizaki K,Yamada A,Arakaki M,Harada H,Nonaka K,Nakamura S,Yamada Y,Fukumoto S

Ameloblastin and amelogenin share a common secretory pathway and are co-secreted during enamel formation.
Matrix biology : journal of the International Society for Matrix Biology27(4)352-9(2008 May)
Zalzal SF,Smith CE,Nanci A

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Ambn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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