Cstb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cstb
Entrez Gene ID	25308
Full Name	cystatin B
Synonyms	Cyb,Epm1,Stfb
General protein information	Preferred Names cystatin B Names cystatin-B cystatin B (stefin B) cystatin beta liver thiol proteinase inhibitor stefin-B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	inhibits the activity of cathepsins B, H, L, and S; may protect against apoptosis that occurs in response to seizures [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012838.2	NP_036970.1	cystatin-B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cstb	NP_031819.1
Gallus gallus (chicken)	CSTB	NP_001185577.1
Danio rerio (zebrafish)	zgc:56530	NP_001096599.1
	CSTB	XP_001104524.1
Bos taurus (cattle)	CSTB	NP_001032538.1
Danio rerio (zebrafish)	zgc:153129	NP_001070742.1
Danio rerio (zebrafish)	si:ch211-161h7.6	XP_698401.1
Rattus norvegicus (Norway rat)	Cstb	NP_036970.1
Homo sapiens (human)	CSTB	NP_000091.1
Pan troglodytes (chimpanzee)	CSTB	NP_001009095.1

Gene Ontology
Bibliography

Related articles in PubMed

Cystatin B and SOD1: protein?protein interaction and possible relation to neurodegeneration.
Ulbrich L, Cozzolino M, Marini ES, Amori I, De Jaco A, Carr? MT, Augusti-Tocco G
Cellular and molecular neurobiology34(2)205-13(2014 Mar)

Insights in progressive myoclonus epilepsy: HSP70 promotes cystatin B polymerization.
Rispoli A, Cipollini E, Catania S, Di Giaimo R, Pulice G, van Houte S, Sparla F, Dal Piaz F, Roncarati D, Trost P, Melli M
Biochimica et biophysica acta1834(12)2591-9(2013 Dec)

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Pieragostino D, Agnifili L, Fasanella V, D'Aguanno S, Mastropasqua R, Di Ilio C, Sacchetta P, Urbani A, Del Boccio P
Molecular bioSystems9(6)1108-16(2013 Jun)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A reciprocal influence of CSTB and SOD1 at the gene expression level and for a direct interaction of the two proteins, is reported.
Title: Cystatin B and SOD1: protein?protein interaction and possible relation to neurodegeneration.

Cu(2+) modifies the redox environment of the protein, allowing the oxidation of the cysteine residue of CSTB that triggers polymerization.
Title: Insights in progressive myoclonus epilepsy: HSP70 promotes cystatin B polymerization.

expression of CSTB in neural stem cells and in differentiating mature brain cells
Title: Cystatin-B is expressed by neural stem cells and by differentiated neurons and astrocytes.

The following Cstb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cstb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12095	NM_012838.2 Latest version!	Rattus norvegicus cystatin B (Cstb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cstb ( NM_012838.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036970.1 Rattus norvegicus cystatin B (Cstb), mRNA.

Price & Availability↑

CloneID	ORa12095
Clone ID Related Accession (Same CDS sequence)	NM_012838.2
Accession Version	NM_012838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	cystatin-B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CF976177.1 and BC084725.1. On Oct 20, 2007 this sequence version replaced NM_012838.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC084725.1, CO402879.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGTGTG GCGCGCCATC CGCCACAATG CCGGCCACGA CCGAGACGCA GGAGATCGCC 
GACAAGGTGA AGTCTCAACT TGAAGAGAAA GCAAATCAGA AGTTTGATGT CTTTAAAGCC 
ATATCCTTCA GGAGACAGGT AGTGGCCGGC ACCAACTTCT TCATCAAGGT TGATGTCGGC 
GAAGAAAAAT GTGTGCACTT GAGGGTGTTT GAACCCCTCC CTCATGAGAA CAAGCCTTTG 
ACCTTGTCTT CTTACCAGAC CGACAAAGAA AAGCACGATG AGCTAACCTA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036970.1
CDS	98..394
Translation	MMCGAPSATMPATTETQEIADKVKSQLEEKANQKFDVFKAISFRRQVVAGTNFFIKVDVGEEKCVHLRVFEPLPHENKPLTLSSYQTDKEKHDELTYF

Target ORF information:

RefSeq Version	NM_012838.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cystatin B (Cstb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012838.2

ORF Insert Sequence:

ATGATGTGTG GCGCGCCATC CGCCACAATG CCGGCCACGA CCGAGACGCA GGAGATCGCC 
GACAAGGTGA AGTCTCAACT TGAAGAGAAA GCAAATCAGA AGTTTGATGT CTTTAAAGCC 
ATATCCTTCA GGAGACAGGT AGTGGCCGGC ACCAACTTCT TCATCAAGGT TGATGTCGGC 
GAAGAAAAAT GTGTGCACTT GAGGGTGTTT GAACCCCTCC CTCATGAGAA CAAGCCTTTG 
ACCTTGTCTT CTTACCAGAC CGACAAAGAA AAGCACGATG AGCTAACCTA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cystatin B and SOD1: protein?protein interaction and possible relation to neurodegeneration.
Cellular and molecular neurobiology34(2)205-13(2014 Mar)
Ulbrich L,Cozzolino M,Marini ES,Amori I,De Jaco A,Carr? MT,Augusti-Tocco G

Insights in progressive myoclonus epilepsy: HSP70 promotes cystatin B polymerization.
Biochimica et biophysica acta1834(12)2591-9(2013 Dec)
Rispoli A,Cipollini E,Catania S,Di Giaimo R,Pulice G,van Houte S,Sparla F,Dal Piaz F,Roncarati D,Trost P,Melli M

Shotgun proteomics reveals specific modulated protein patterns in tears of patients with primary open angle glaucoma na?ve to therapy.
Molecular bioSystems9(6)1108-16(2013 Jun)
Pieragostino D,Agnifili L,Fasanella V,D'Aguanno S,Mastropasqua R,Di Ilio C,Sacchetta P,Urbani A,Del Boccio P

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

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Cstb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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