Cd247 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd247
Entrez Gene ID	25300
Full Name	Cd247 molecule
Synonyms	Cd3z,TCRzeta
General protein information	Preferred Names Cd247 molecule Names T-cell surface glycoprotein CD3 zeta chain CD247 antigen CD3 antigen, zeta polypeptide T-cell receptor CD3, subunit zeta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q23
Summary	component of T cell receptor (TCR) complex that plays a role in TCR assembly and signaling; does not promote surface expression of Fc gamma RIII, unlike human homolog [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006250147.3	XP_006250209.1	T-cell surface glycoprotein CD3 zeta chain isoform X1
NM_001205304.1	NP_001192233.1	T-cell surface glycoprotein CD3 zeta chain isoform 2 precursor
NM_170789.2	NP_740770.2	T-cell surface glycoprotein CD3 zeta chain isoform 1 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CD247	NP_996762.1
Pan troglodytes (chimpanzee)	CD247	XP_001174731.1
Bos taurus (cattle)	CD247	NP_776437.1
Rattus norvegicus (Norway rat)	Cd247	NP_740770.2
Xenopus tropicalis (tropical clawed frog)	cd247	XP_002936278.1
	CD247	NP_001070891.1
Homo sapiens (human)	CD247	NP_932170.1
Canis lupus familiaris (dog)	CD247	XP_005623027.1
Mus musculus (house mouse)	Cd247	NP_001106862.1

Gene Ontology
Bibliography

Related articles in PubMed

Changes in Expressions of Major Histocompatibility Complex Class I, Paired-Immunoglobulin-Like Receptor B, and Cluster of Differentiation 3? in Motor Cortical Representations of the Brachial Plexus After Avulsion in Rats.
Zhang J, Chen L, Gu YD
World neurosurgery106211-218(2017 Oct)

CD247 modulates blood pressure by altering T-lymphocyte infiltration in the kidney.
Rudemiller N, Lund H, Jacob HJ, Geurts AM, Mattson DL,
Hypertension (Dallas, Tex. : 1979)63(3)559-64(2014 Mar)

?-Arrestin-1 mediates the TCR-triggered re-routing of distal receptors to the immunological synapse by a PKC-mediated mechanism.
Fern?ndez-Arenas E, Calleja E, Mart?nez-Mart?n N, Gharbi SI, Navajas R, Garc?a-Medel N, Penela P, Alcam? A, Mayor F Jr, Albar JP, Alarc?n B
The EMBO journal33(6)559-77(2014 Mar)

Ectopic expression of the immune adaptor protein CD3zeta in neural stem/progenitor cells disrupts cell-fate specification.
Angibaud J, Baudouin SJ, Louveau A, Nerri?re-Daguin V, Bonnamain V, Csaba Z, Dournaud P, Naveilhan P, Noraz N, Pellier-Monnin V, Boudin H
Journal of molecular neuroscience : MN46(2)431-41(2012 Feb)

The signaling adaptor protein CD3zeta is a negative regulator of dendrite development in young neurons.
Baudouin SJ, Angibaud J, Loussouarn G, Bonnamain V, Matsuura A, Kinebuchi M, Naveilhan P, Boudin H
Molecular biology of the cell19(6)2444-56(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In the original motor cortical representations of the brachial plexus, mRNA and protein expression levels of MHCI, PirB, and CD3zeta were down-regulated 7 days post brachial plexus injury compared with control. Interestingly, mRNA and protein expression levels of these factors were up-regulated at 3 months compared with 7 days, excepting PirB protein, whose expression was not increasing.
Title: Changes in Expressions of Major Histocompatibility Complex Class I, Paired-Immunoglobulin-Like Receptor B, and Cluster of Differentiation 3? in Motor Cortical Representations of the Brachial Plexus After Avulsion in Rats.

functional T cells are required for the full development of Dahl salt-sensitive hypertension and indicate that the association between CD247 and hypertension in humans may be related to altered immune cell function.
Title: CD247 modulates blood pressure by altering T-lymphocyte infiltration in the kidney.

ectopic CD3zeta expression in neural precursor stem cells impaired their normal cell-fate specification. Variations of CD3zeta expression in the developing CNS might result in neurodevelopmental anomalies.
Title: Ectopic expression of the immune adaptor protein CD3zeta in neural stem/progenitor cells disrupts cell-fate specification.

The transmembrane adaptor signaling protein CD3zeta, first described in the immune system, has a previously uncharacterized role in regulating neuronal development.
Title: The signaling adaptor protein CD3zeta is a negative regulator of dendrite development in young neurons.

Tere is a robust postnatal up-regulation of CD3-zeta in rat hippocampus in early postnatal period.
Title: Effect of TNF-alpha on CD3-zeta and MHC-I in postnatal rat hippocampus.

The following Cd247 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd247 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12949	NM_170789.2 Latest version!	Rattus norvegicus Cd247 molecule (Cd247), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa12950	NM_001205304.1 Latest version!	Rattus norvegicus Cd247 molecule (Cd247), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa35550	XM_006250147.3 Latest version!	Rattus norvegicus Cd247 molecule (Cd247), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd247 ( NM_170789.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_740770.2 Rattus norvegicus Cd247 molecule (Cd247), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12949
Clone ID Related Accession (Same CDS sequence)	NM_170789.2
Accession Version	NM_170789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	T-cell surface glycoprotein CD3 zeta chain isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07021649.1 and BC097933.1. On Apr 13, 2011 this sequence version replaced NM_170789.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D13555.1, L08447.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTGGA CGGCATCAGT CCTCGCCTGC ATCCTTCAAG TGCAGTTCCC AGGAGCAGAG 
GCACAGAGCT TTGGTCTGCT GGATCCCAAA CTCTGCTATA TGCTAGATGG AATCCTCTTC 
ATCTACGGAG TCATCGTCAC GGCCCTGTAC CTGAGAGCAA AATTCAGCAG GAGTGCAGAT 
GCTGCTGCTT ACCTTCAGGA CCCCAACCAG CTCTATAACG AGCTCAACCT AGGGCGAAGA 
GAGGAATATG ATGTTTTGGA CAAGAAGCGG CCTCGGGATC CGGAGATGGG GGGCAAACAG 
CAGAGGAGGA GGAACCCCCA GGAAGGCGTG TACAATGCAC TGCAGAAAGA CAAGATGGCA 
GAGGCCTACA GTGAGATTGG CATGAAAGGC GAGAGGCGGA GAGGCAAGGG GCACGACGGC 
CTTTACCAGG GTCTCAGCAC TGCCACCAAG GACACCTATG ACGCCCTGCA TATGCAGACC 
CTGCCCCCTC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_740770.2
CDS	120..614
Translation	MKWTASVLACILQVQFPGAEAQSFGLLDPKLCYMLDGILFIYGVIVTALYLRAKFSRSADAAAYLQDPNQLYNELNLGRREEYDVLDKKRPRDPEMGGKQQRRRNPQEGVYNALQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQTLPPR

Target ORF information:

RefSeq Version	NM_170789.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cd247 molecule (Cd247), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170789.2

ORF Insert Sequence:

ATGAAGTGGA CGGCATCAGT CCTCGCCTGC ATCCTTCAAG TGCAGTTCCC AGGAGCAGAG 
GCACAGAGCT TTGGTCTGCT GGATCCCAAA CTCTGCTATA TGCTAGATGG AATCCTCTTC 
ATCTACGGAG TCATCGTCAC GGCCCTGTAC CTGAGAGCAA AATTCAGCAG GAGTGCAGAT 
GCTGCTGCTT ACCTTCAGGA CCCCAACCAG CTCTATAACG AGCTCAACCT AGGGCGAAGA 
GAGGAATATG ATGTTTTGGA CAAGAAGCGG CCTCGGGATC CGGAGATGGG GGGCAAACAG 
CAGAGGAGGA GGAACCCCCA GGAAGGCGTG TACAATGCAC TGCAGAAAGA CAAGATGGCA 
GAGGCCTACA GTGAGATTGG CATGAAAGGC GAGAGGCGGA GAGGCAAGGG GCACGACGGC 
CTTTACCAGG GTCTCAGCAC TGCCACCAAG GACACCTATG ACGCCCTGCA TATGCAGACC 
CTGCCCCCTC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd247 ( NM_001205304.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001192233.1 Rattus norvegicus Cd247 molecule (Cd247), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12950
Clone ID Related Accession (Same CDS sequence)	NM_001205304.1
Accession Version	NM_001205304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	T-cell surface glycoprotein CD3 zeta chain isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07021649.1, CO562119.1 and BC097933.1. Transcript Variant: This variant (2) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CO562119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689603 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTGGA CGGCATCAGT CCTCGCCTGC ATCCTTCAAG TGCAGTTCCC AGGAGCAGAG 
GCACAGAGCT TTGGTCTGCT GGATCCCAAA CTCTGCTATA TGCTAGATGG AATCCTCTTC 
ATCTACGGAG TCATCGTCAC GGCCCTGTAC CTGAGAGCAA AATTCAGCAG GAGTGCAGAT 
GCTGCTGCTT ACCTTCAGGA CCCCAACCAG CTCTATAACC AGAGGAGGAG GAACCCCCAG 
GAAGGCGTGT ACAATGCACT GCAGAAAGAC AAGATGGCAG AGGCCTACAG TGAGATTGGC 
ATGAAAGGCG AGAGGCGGAG AGGCAAGGGG CACGACGGCC TTTACCAGGG TCTCAGCACT 
GCCACCAAGG ACACCTATGA CGCCCTGCAT ATGCAGACCC TGCCCCCTCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192233.1
CDS	120..533
Translation	MKWTASVLACILQVQFPGAEAQSFGLLDPKLCYMLDGILFIYGVIVTALYLRAKFSRSADAAAYLQDPNQLYNQRRRNPQEGVYNALQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQTLPPR

Target ORF information:

RefSeq Version	NM_001205304.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cd247 molecule (Cd247), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205304.1

ORF Insert Sequence:

ATGAAGTGGA CGGCATCAGT CCTCGCCTGC ATCCTTCAAG TGCAGTTCCC AGGAGCAGAG 
GCACAGAGCT TTGGTCTGCT GGATCCCAAA CTCTGCTATA TGCTAGATGG AATCCTCTTC 
ATCTACGGAG TCATCGTCAC GGCCCTGTAC CTGAGAGCAA AATTCAGCAG GAGTGCAGAT 
GCTGCTGCTT ACCTTCAGGA CCCCAACCAG CTCTATAACC AGAGGAGGAG GAACCCCCAG 
GAAGGCGTGT ACAATGCACT GCAGAAAGAC AAGATGGCAG AGGCCTACAG TGAGATTGGC 
ATGAAAGGCG AGAGGCGGAG AGGCAAGGGG CACGACGGCC TTTACCAGGG TCTCAGCACT 
GCCACCAAGG ACACCTATGA CGCCCTGCAT ATGCAGACCC TGCCCCCTCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cd247 ( XM_006250147.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250209.1 Rattus norvegicus Cd247 molecule (Cd247), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35550
Clone ID Related Accession (Same CDS sequence)	XM_006250147.3
Accession Version	XM_006250147.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	T-cell surface glycoprotein CD3 zeta chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGGA CGGCATCAGT CCTCGCCTGC ATCCTTCAAG TGCAGTTCCC AGGAGCAGAG 
GCACAGAGCT TTGGTCTGCT GGATCCCAAA CTCTGCTATA TGCTAGATGG AATCCTCTTC 
ATCTACGGAG TCATCGTCAC GGCCCTGTAC CTGAGAGCAA AATTCAGCAG GAGTGCAGAT 
GCTGCTGCTT ACCTTCAGGA CCCCAACCAG CTCTATAACG AGCTCAACCT AGGGCGAAGA 
GAGGAATATG ATGTTTTGGA CAAGAAGCGG CCTCGGGATC CGGAGATGGG GGGCAAACAG 
AGGAGGAGGA ACCCCCAGGA AGGCGTGTAC AATGCACTGC AGAAAGACAA GATGGCAGAG 
GCCTACAGTG AGATTGGCAT GAAAGGCGAG AGGCGGAGAG GCAAGGGGCA CGACGGCCTT 
TACCAGGGTC TCAGCACTGC CACCAAGGAC ACCTATGACG CCCTGCATAT GCAGACCCTG 
CCCCCTCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250209.1
CDS	155..646
Translation	MKWTASVLACILQVQFPGAEAQSFGLLDPKLCYMLDGILFIYGVIVTALYLRAKFSRSADAAAYLQDPNQLYNELNLGRREEYDVLDKKRPRDPEMGGKQRRRNPQEGVYNALQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQTLPPR

Target ORF information:

RefSeq Version	XM_006250147.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Cd247 molecule (Cd247), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250147.3

ORF Insert Sequence:

ATGAAGTGGA CGGCATCAGT CCTCGCCTGC ATCCTTCAAG TGCAGTTCCC AGGAGCAGAG 
GCACAGAGCT TTGGTCTGCT GGATCCCAAA CTCTGCTATA TGCTAGATGG AATCCTCTTC 
ATCTACGGAG TCATCGTCAC GGCCCTGTAC CTGAGAGCAA AATTCAGCAG GAGTGCAGAT 
GCTGCTGCTT ACCTTCAGGA CCCCAACCAG CTCTATAACG AGCTCAACCT AGGGCGAAGA 
GAGGAATATG ATGTTTTGGA CAAGAAGCGG CCTCGGGATC CGGAGATGGG GGGCAAACAG 
AGGAGGAGGA ACCCCCAGGA AGGCGTGTAC AATGCACTGC AGAAAGACAA GATGGCAGAG 
GCCTACAGTG AGATTGGCAT GAAAGGCGAG AGGCGGAGAG GCAAGGGGCA CGACGGCCTT 
TACCAGGGTC TCAGCACTGC CACCAAGGAC ACCTATGACG CCCTGCATAT GCAGACCCTG 
CCCCCTCGCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Changes in Expressions of Major Histocompatibility Complex Class I, Paired-Immunoglobulin-Like Receptor B, and Cluster of Differentiation 3? in Motor Cortical Representations of the Brachial Plexus After Avulsion in Rats.
World neurosurgery106211-218(2017 Oct)
Zhang J,Chen L,Gu YD

CD247 modulates blood pressure by altering T-lymphocyte infiltration in the kidney.
Hypertension (Dallas, Tex. : 1979)63(3)559-64(2014 Mar)
Rudemiller N,Lund H,Jacob HJ,Geurts AM,Mattson DL,

?-Arrestin-1 mediates the TCR-triggered re-routing of distal receptors to the immunological synapse by a PKC-mediated mechanism.
The EMBO journal33(6)559-77(2014 Mar)
Fern?ndez-Arenas E,Calleja E,Mart?nez-Mart?n N,Gharbi SI,Navajas R,Garc?a-Medel N,Penela P,Alcam? A,Mayor F Jr,Albar JP,Alarc?n B

Ectopic expression of the immune adaptor protein CD3zeta in neural stem/progenitor cells disrupts cell-fate specification.
Journal of molecular neuroscience : MN46(2)431-41(2012 Feb)
Angibaud J,Baudouin SJ,Louveau A,Nerri?re-Daguin V,Bonnamain V,Csaba Z,Dournaud P,Naveilhan P,Noraz N,Pellier-Monnin V,Boudin H

The signaling adaptor protein CD3zeta is a negative regulator of dendrite development in young neurons.
Molecular biology of the cell19(6)2444-56(2008 Jun)
Baudouin SJ,Angibaud J,Loussouarn G,Bonnamain V,Matsuura A,Kinebuchi M,Naveilhan P,Boudin H

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Cd247 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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