Cyp3a18 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp3a18
Entrez Gene ID	252931
Full Name	cytochrome P450, family 3, subfamily a, polypeptide 18
General protein information	Preferred Names cytochrome P450, family 3, subfamily a, polypeptide 18 Names cytochrome P450 3A18 CYPIIIA18 P450(6)beta-2 cytochrome P450(6)beta-2 cytochrome P450, 3a18
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p11
Summary	catalyzes 16 beta- and 6-alpha hydroxylations of testosterone [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_145782.1	NP_665725.1	cytochrome P450 3A18

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cyp3a57	NP_001093650.1
Rattus norvegicus (Norway rat)	Cyp3a18	NP_665725.1
Neurospora crassa	NCU01086	XP_961698.2
Pan troglodytes (chimpanzee)	CYP3A7	NP_001087243.1
Mus musculus (house mouse)	Cyp3a25	NP_062766.2
Mus musculus (house mouse)	Cyp3a59	NP_001098630.1
Magnaporthe oryzae (rice blast fungus)	MGG_13928	XP_003713223.1

Gene Ontology
Bibliography

Related articles in PubMed

Acetaminophen bioactivation by human cytochrome P450 enzymes and animal microsomes.
Laine JE, Auriola S, Pasanen M, Juvonen RO
Xenobiotica; the fate of foreign compounds in biological systems39(1)11-21(2009 Jan)

The relative contribution of human cytochrome P450 isoforms to the four caffeine oxidation pathways: an in vitro comparative study with cDNA-expressed P450s including CYP2C isoforms.
Kot M, Daniel WA
Biochemical pharmacology76(4)543-51(2008 Aug)

Identification of the human cytochrome P450 enzymes involved in the in vitro biotransformation of lynestrenol and norethindrone.
Korhonen T, Turpeinen M, Tolonen A, Laine K, Pelkonen O
The Journal of steroid biochemistry and molecular biology110(1-2)56-66(2008 May)

An NH2-terminal truncated cytochrome P450 CYP3A4 showing catalytic activity is present in the cytoplasm of human liver cells.
Jeon S, Kim KH, Yun CH, Hong BW, Chang YS, Han HS, Yoon YS, Choi WB, Kim S, Lee AY
Experimental & molecular medicine40(2)254-60(2008 Apr)

Radical intermediates in the catalytic oxidation of hydrocarbons by bacterial and human cytochrome P450 enzymes.
Jiang Y, He X, Ortiz de Montellano PR
Biochemistry45(2)533-42(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cyp3a18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp3a18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02713	NM_145782.1 Latest version!	Rattus norvegicus cytochrome P450, family 3, subfamily a, polypeptide 18 (Cyp3a18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp3a18 ( NM_145782.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_665725.1 Rattus norvegicus cytochrome P450, family 3, subfamily a, polypeptide 18 (Cyp3a18), mRNA.

Price & Availability↑

CloneID	ORa02713
Clone ID Related Accession (Same CDS sequence)	NM_145782.1
Accession Version	NM_145782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1494bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 3A18
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X79991.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X79991.1, D38381.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGGAGATCA TTCCCAACCT TTCTATAGAG ACCTGGGTGC TTCTAGCTAC TAGCTTGATG 
CTCTTCTACA TATATGGGAC CTATTCTCAT GGCCTGTTTA AGAAACTAGG AATTCCTGGA 
CCCAAACCTG TGCCTTTATT TGGCACCATT TTCAACTACG GTGATGGCAT GTGGAAATTT 
GATGATGACT GCTATAAAAA GTATGGAAAA ATATGGGGGT TTTATGAGGG CCCACAGCCT 
TTTTTGGCTA TCATGGATCC AGAGATCATC AAAATGGTGC TGGTGAAAGA ATGTTACTCA 
GTCTTCACAA ACCGTCGGTG TTTTGGGCCA ATGGGATTTA TGAAAAAGGC CATTACCATG 
TCTGAGGATG AAGAATGGAA GAGACTTCGA ACAATCCTGT CTCCAACCTT CACCAGTGGC 
AAACTCAAGG AGATGTTCCC CCTCATGAGA CAGTATGGAG ATACATTGTT GAAGAACTTG 
AGGCGAGAAG AAGCAAAAGG GGAGCCCATC AACATGAAAG ACATCTTTGG AGCTTATAGC 
ATGGACGTGA TCACTGGCAC ATCATTTGGA GTGAACGTCG ATTCCCTCAA CAATCCACAG 
GATCCCTTCG TGCAGAAAGC CAAGAAGATC TTAAAATTTC AAATTTTTGA TCCATTTCTT 
CTCTCTGTAG TTCTGTTTCC ATTTCTTACT CCAATATATG AGATGTTAAA TTTTTCAATT 
TTTCCAAGAC AGTCAATGAA CTTTTTCAAA AAATTCGTAA AAACAATGAA GAAAAATCGC 
CTTGATTCAA ACCAGAAGAA CCGAGTGGAT TTTCTTCAAC TGATGATGAA TACTCAGAAC 
TCCAAAGGCC AAGAGTCCCA GAAAGCTCTT TCTGATCTAG AAATGGCAGC ACAAGCTATT 
ATTTTCATTT TTGGGGGTTA TGATGCCACA AGCACCTCCA TTTCCTTCAT AATGTATGAA 
CTGGCCACTC GCCCCAATGT GCAAAAGAAA CTCCAGAATG AGATTGATAG AGCTCTGCCC 
AATAAGGCAC CTGTCACCTA TGATGCTCTG ATGGAAATGG AGTACCTGGA CATGGTGGTG 
AATGAAAGTC TAAGATTGTA CCCAATTGCT ACCAGGCTAG ACAGAGTCTC AAAAAAGGAT 
GTGGAAATCA ATGGAGTTTT TATTCCCAAA GGGACTGTAG TTACGATACC AATCTATCCT 
CTTCATCGGA ACCCTGAGTA CTGGCTAGAG CCTGAGGAAT TCAACCCTGA AAGGTTCAGC 
AAGGAGAACA AGGGCAGCAT TGATCCTTAT GTATATCTGC CCTTTGGAAA TGGACCCAGG 
AACTGCATTG GCATGAGGTT TGCTCTCATC AGCATGAAAC TTGCTGTCAT AGGAGTCCTG 
CAGAACTTCA ATATCCAGCC TTGTGAGAAG ACACAGATCC CTCTGAAGAT CAGTAGGCAA 
CCAATTTTCC AACCAGAAGG ACCCATCATC CTAAAGCTTG TGTCAAGAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665725.1
CDS	60..1553
Translation	MEIIPNLSIETWVLLATSLMLFYIYGTYSHGLFKKLGIPGPKPVPLFGTIFNYGDGMWKFDDDCYKKYGKIWGFYEGPQPFLAIMDPEIIKMVLVKECYSVFTNRRCFGPMGFMKKAITMSEDEEWKRLRTILSPTFTSGKLKEMFPLMRQYGDTLLKNLRREEAKGEPINMKDIFGAYSMDVITGTSFGVNVDSLNNPQDPFVQKAKKILKFQIFDPFLLSVVLFPFLTPIYEMLNFSIFPRQSMNFFKKFVKTMKKNRLDSNQKNRVDFLQLMMNTQNSKGQESQKALSDLEMAAQAIIFIFGGYDATSTSISFIMYELATRPNVQKKLQNEIDRALPNKAPVTYDALMEMEYLDMVVNESLRLYPIATRLDRVSKKDVEINGVFIPKGTVVTIPIYPLHRNPEYWLEPEEFNPERFSKENKGSIDPYVYLPFGNGPRNCIGMRFALISMKLAVIGVLQNFNIQPCEKTQIPLKISRQPIFQPEGPIILKLVSRD

Target ORF information:

RefSeq Version	NM_145782.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 3, subfamily a, polypeptide 18 (Cyp3a18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145782.1

ORF Insert Sequence:

ATGGAGATCA TTCCCAACCT TTCTATAGAG ACCTGGGTGC TTCTAGCTAC TAGCTTGATG 
CTCTTCTACA TATATGGGAC CTATTCTCAT GGCCTGTTTA AGAAACTAGG AATTCCTGGA 
CCCAAACCTG TGCCTTTATT TGGCACCATT TTCAACTACG GTGATGGCAT GTGGAAATTT 
GATGATGACT GCTATAAAAA GTATGGAAAA ATATGGGGGT TTTATGAGGG CCCACAGCCT 
TTTTTGGCTA TCATGGATCC AGAGATCATC AAAATGGTGC TGGTGAAAGA ATGTTACTCA 
GTCTTCACAA ACCGTCGGTG TTTTGGGCCA ATGGGATTTA TGAAAAAGGC CATTACCATG 
TCTGAGGATG AAGAATGGAA GAGACTTCGA ACAATCCTGT CTCCAACCTT CACCAGTGGC 
AAACTCAAGG AGATGTTCCC CCTCATGAGA CAGTATGGAG ATACATTGTT GAAGAACTTG 
AGGCGAGAAG AAGCAAAAGG GGAGCCCATC AACATGAAAG ACATCTTTGG AGCTTATAGC 
ATGGACGTGA TCACTGGCAC ATCATTTGGA GTGAACGTCG ATTCCCTCAA CAATCCACAG 
GATCCCTTCG TGCAGAAAGC CAAGAAGATC TTAAAATTTC AAATTTTTGA TCCATTTCTT 
CTCTCTGTAG TTCTGTTTCC ATTTCTTACT CCAATATATG AGATGTTAAA TTTTTCAATT 
TTTCCAAGAC AGTCAATGAA CTTTTTCAAA AAATTCGTAA AAACAATGAA GAAAAATCGC 
CTTGATTCAA ACCAGAAGAA CCGAGTGGAT TTTCTTCAAC TGATGATGAA TACTCAGAAC 
TCCAAAGGCC AAGAGTCCCA GAAAGCTCTT TCTGATCTAG AAATGGCAGC ACAAGCTATT 
ATTTTCATTT TTGGGGGTTA TGATGCCACA AGCACCTCCA TTTCCTTCAT AATGTATGAA 
CTGGCCACTC GCCCCAATGT GCAAAAGAAA CTCCAGAATG AGATTGATAG AGCTCTGCCC 
AATAAGGCAC CTGTCACCTA TGATGCTCTG ATGGAAATGG AGTACCTGGA CATGGTGGTG 
AATGAAAGTC TAAGATTGTA CCCAATTGCT ACCAGGCTAG ACAGAGTCTC AAAAAAGGAT 
GTGGAAATCA ATGGAGTTTT TATTCCCAAA GGGACTGTAG TTACGATACC AATCTATCCT 
CTTCATCGGA ACCCTGAGTA CTGGCTAGAG CCTGAGGAAT TCAACCCTGA AAGGTTCAGC 
AAGGAGAACA AGGGCAGCAT TGATCCTTAT GTATATCTGC CCTTTGGAAA TGGACCCAGG 
AACTGCATTG GCATGAGGTT TGCTCTCATC AGCATGAAAC TTGCTGTCAT AGGAGTCCTG 
CAGAACTTCA ATATCCAGCC TTGTGAGAAG ACACAGATCC CTCTGAAGAT CAGTAGGCAA 
CCAATTTTCC AACCAGAAGG ACCCATCATC CTAAAGCTTG TGTCAAGAGA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Acetaminophen bioactivation by human cytochrome P450 enzymes and animal microsomes.
Xenobiotica; the fate of foreign compounds in biological systems39(1)11-21(2009 Jan)
Laine JE,Auriola S,Pasanen M,Juvonen RO

The relative contribution of human cytochrome P450 isoforms to the four caffeine oxidation pathways: an in vitro comparative study with cDNA-expressed P450s including CYP2C isoforms.
Biochemical pharmacology76(4)543-51(2008 Aug)
Kot M,Daniel WA

Identification of the human cytochrome P450 enzymes involved in the in vitro biotransformation of lynestrenol and norethindrone.
The Journal of steroid biochemistry and molecular biology110(1-2)56-66(2008 May)
Korhonen T,Turpeinen M,Tolonen A,Laine K,Pelkonen O

An NH2-terminal truncated cytochrome P450 CYP3A4 showing catalytic activity is present in the cytoplasm of human liver cells.
Experimental & molecular medicine40(2)254-60(2008 Apr)
Jeon S,Kim KH,Yun CH,Hong BW,Chang YS,Han HS,Yoon YS,Choi WB,Kim S,Lee AY

Radical intermediates in the catalytic oxidation of hydrocarbons by bacterial and human cytochrome P450 enzymes.
Biochemistry45(2)533-42(2006 Jan)
Jiang Y,He X,Ortiz de Montellano PR