Rapgef4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rapgef4
Entrez Gene ID	252857
Full Name	Rap guanine nucleotide exchange factor 4
Synonyms	CAMP-GEFII,CAMPS,Cgef2,Epac2
General protein information	Preferred Names Rap guanine nucleotide exchange factor 4 Names rap guanine nucleotide exchange factor 4 Rap guanine nucleotide exchange factor (GEF) 4 cAMP-regulated guanine nucleotide exchange factor II epac 2 exchange factor directly activated by cAMP 2 exchange protein directly activated by cAMP 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q23

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100642.1	NP_001094112.1	rap guanine nucleotide exchange factor 4
XM_001060956.5	XP_001060956.2	rap guanine nucleotide exchange factor 4 isoform X2
XM_017592164.1	XP_017447653.1	rap guanine nucleotide exchange factor 4 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rapgef4	XP_006234430.1
Xenopus tropicalis (tropical clawed frog)	rapgef4	XP_002934663.2
Pan troglodytes (chimpanzee)	RAPGEF4	XP_003309469.1
Canis lupus familiaris (dog)	RAPGEF4	XP_005640372.1
Mus musculus (house mouse)	Rapgef4	NP_001191094.1
Homo sapiens (human)	RAPGEF4	NP_008954.2
	RAPGEF4	XP_001086678.2
Bos taurus (cattle)	RAPGEF4	NP_001179831.1
Gallus gallus (chicken)	RAPGEF4	XP_426579.3
Danio rerio (zebrafish)	rapgef4	XP_687578.4
Drosophila melanogaster (fruit fly)	Epac	NP_001097202.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007307	XP_308514.4
Caenorhabditis elegans (roundworm)	epac-1	NP_499256.2

Gene Ontology
Bibliography

Related articles in PubMed

Guanine nucleotide exchange factor Epac2-dependent activation of the GTP-binding protein Rap2A mediates cAMP-dependent growth arrest in neuroendocrine cells.
Emery AC, Xu W, Eiden MV, Eiden LE
The Journal of biological chemistry292(29)12220-12231(2017 07)

Exchange proteins directly activated by cAMP induce the proliferation of rat anterior pituitary GH3 cells via the activation of extracellular signal-regulated kinase.
Sun W, Jiao W, Huang Y, Li R, Zhang Z, Wang J, Lei T
Biochemical and biophysical research communications485(2)355-359(2017 04)

EPAC activation inhibits acetaldehyde-induced activation and proliferation of hepatic stellate cell via Rap1.
Yang Y, Yang F, Wu X, Lv X, Li J
Canadian journal of physiology and pharmacology94(5)498-507(2016 May)

Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus.
Fernandes HB, Riordan S, Nomura T, Remmers CL, Kraniotis S, Marshall JJ, Kukreja L, Vassar R, Contractor A
The Journal of neuroscience : the official journal of the Society for Neuroscience35(16)6544-53(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Rap2A is the obligate effector for Epac2 in mediating growth arrest and reversal of neurite extension through activation of MAP kinase signaling via protein phosphorylation/farnesylation in neuroendocrine cells. (Rap2A = RAS oncogene family member Rap2A; Epac2 = guanine nucleotide exchange factor Epac2)
Title: Guanine nucleotide exchange factor Epac2-dependent activation of the GTP-binding protein Rap2A mediates cAMP-dependent growth arrest in neuroendocrine cells.

Rap guanine nucleotide exchange factors Epac1 and Epac2 induces the phosphorylation of extracellular signal-regulated kinases ERK1/2 in pituitary tumor GH3 cells.
Title: Exchange proteins directly activated by cAMP induce the proliferation of rat anterior pituitary GH3 cells via the activation of extracellular signal-regulated kinase.

Data suggest activation of EPAC1/2 (exchange proteins activated by cAMP) and Rap1 (RAS-related GTP-binding protein 1) in hepatic stellate cells down-regulates proliferation and synthesis of a-smooth muscle actin/collagen I/collagen II in an in vitro model of alcoholic cirrhosis (AC); in AC, expression of EPAC1 is down-regulated but expression of EPAC2 is up-regulated; EPAC1 activation increases GTP-bound (activated) Rap1.
Title: EPAC activation inhibits acetaldehyde-induced activation and proliferation of hepatic stellate cell via Rap1.

Suggest that EPAC1/2 and RAP1 are the crucial factors for endometrial decidualization in rat pregnancy.
Title: Possible roles of the cAMP-mediators EPAC and RAP1 in decidualization of rat uterus.

Inhibition of adenylate cyclase (SQ-22536) abolished the positive interaction between stimuli and the Epac (exchange proteins activated by cAMP) activator 8-pCPT-2'-O-Me-cAMP reversed the effect of adenylate cyclase inhibition
Title: Cyclic AMP and Epac contribute to the genesis of the positive interaction between hypoxia and hypercapnia in the carotid body.

The following Rapgef4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rapgef4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa15670	NM_001100642.1 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa15670	XM_017592164.1 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa15670	XM_001060956.5 Latest version!	Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rapgef4 ( NM_001100642.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001094112.1 Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), mRNA.

Price & Availability↑

CloneID	ORa15670
Clone ID Related Accession (Same CDS sequence)	NM_001100642.1 , XM_001060956.5 , XM_017592164.1
Accession Version	NM_001100642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01022633.1, AAHX01022636.1, AAHX01022641.1, AAHX01022642.1, AAHX01022645.1, AAHX01022646.1 and AAHX01022647.1. On Nov 16, 2019 this sequence version replaced XM_001060956.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB031229.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGGTTGCTG CGCACGCTGC ACACTCTCCG TCCTCGGCCG AGTGGATCGC CTGCCTGGAT 
AAAAGGCCGT TGGAGCGATC TAGTGAAGAT GTGGACATAA TTTTCACGCG GCTGAAAGGA 
GTTAAAGCTT TTGAGAGATT TCACCCAAAC CTCCTTCGTC AGATTTGTTT ATGCAGTTAC 
TATGAGAACC TGGAGAAAGG AATCACACTG TTTCGACAAG GGGACATTGG AACCAACTGG 
TATGCTGTCC TAGCTGGGTC TTTGGATGTT AAAGTGTCTG AGACCAGCAG TCACCAGGAT 
GCGGTGACCA TCTGTACCCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTCGACAAC 
ACACCTCGTC ATGCAACCAT TGTTACCAGA GAGAACAGTG AGCTCCTCCG TATCGAGCAG 
GAAGACTTTA AGGCACTATG GGAGAAATAC CGACAGTATA TGGCCGGACT TCTGGCTCCT 
CCCTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCGGACAA GGAGAATACA 
CCTCTCATTG AACCTCACGT TCCTCTCCGC CCTGCTCACA CCATTACCAA GGTCCCTTCA 
GAGAAGATCC TCAGAGCTGG AAAAATTTTG CGAATTGCCA TTCTCTCTCG AGCACCCCAC 
ATGATAAGAG ACAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT TGGGACTGAG 
CTGGTAGACT GGATGATACA GCAGACGTCC TGTGTTCACT CCCGGACTCA AGCTGTTGGC 
ATGTGGCAAG TCTTGCTGGA AGATGGCGTC CTCAACCACG TGGACCAGGA GCGCCATTTC 
CAAGATAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ATGAGGATGC CCCTTTGCCT 
ACTGAGGAGG AGAAGAAGGA GTGCGATGAA GAGCTCCAGG ACACCATGCT GCTGCTGTCA 
CAAATGGGCC CTGACGCCCA CATGAGAATG ATCCTGCGAA AACCACCTGG CCAGAGGACT 
GCAGATGACC TAGAGATTAT CTACGACGAA CTCCTTCATA TTAAAGCCTT ATCCCATCTT 
TCTACCACAG TGAAACGGGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA 
GGAACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATCAT TCTGAAAGGA 
TCCGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC 
TTTGGCAAGT TAGCTCTGGT GAACGATGCT CCGAGAGCTG CATCCATTGT TCTTCGGGAA 
GATAATTGTC ACTTCCTAAG AGTTGACAAG GAAGACTTCA ATCGGATTCT GAGGGACGTT 
GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTACT GGAGAAGGTC 
CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG 
ATGTCAGGAA CACCTGAAAA GATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA 
TCTTTGAATG AAGCAACAGA TTCAGTTTTA AATGACTTCG TTATGATGCA CTGTGTTTTT 
ATGCCAAACA CCCAGCTTTG CCCCGCCCTC GTGGCCCATT ACCACGCACA GCCTTCTCAA 
GGTACAGAAC AGGAGAGAAT GGATTACGCC CTCAACAACA AGAGGCGGGT CATCCGCCTG 
GTTCTGCAAT GGGCAGCCAT GTATGGTGAT CTTCTCCAAG AAGACGACGT GGCCATGGCT 
TTCCTGGAGG AGTTCTATGT GTCTGTATCA GATGATGCAC GGATGATGGT CGCCTTCAAG 
GAGCAGCTGG CTGAGCTGGA GAAGACTGTC AAACAGATCT CAGAAGACGC AAAAGCTCCA 
CAGAAGAAGC ACAAGGTGCT TTTGCAGCAG TTTAATACAG GTGACGAGAG GGCCCAGAAG 
CGTCAGCCGA TTCGTGGCTC TGATGAGGTT TTGTTCAAGG TCTACTGCAT TGACCACACC 
TATACCACCA TCCGAGTGCC TGTAGCCGCC TCGGTGAAGG AAGTCATCAG TGCAGTAGCA 
GACAAACTTG GCTCTGGGGA AGGCCTGATC ATTGTCAAGA TGAACTCTGG AGGAGAAAAG 
GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG 
TTTGCCTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCG 
ACCACTGGGA CAGTGGGGAC GTTTGAACTG ATGAGCTCGA AAGACTTGGC GTACCAGATG 
ACAACGTATG ACTGGGAACT CTTCAACTGT GTGCACGAGC TGGAGCTAAT CTACCACACA 
TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT 
AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCT CAGCAAACGT 
GTTCAGCTTT TGAAAAAATT TATCAAGATA GCGGCTCACT GCAAGGAGTA CAAAAACCTG 
AATTCCTTCT TCGCCATCGT CATGGGGCTC AGTAACGTGG CTGTGAGCCG CCTGGCATTA 
ACATGGGAGA AACTGCCGAG CAAGTTTAAG AAGTTCTATG CGGAGTTTGA GAGCTTAATG 
GATCCTTCCA GAAATCACAG GGCGTACAGG CTGACAGCAG CTAAACTGGA GCCCCCCCTC 
ATCCCTTTCA TGCCCTTGCT TATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA 
TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACG 
GTGCGCTACT ACAGGAGCCA GCCATTCAAT CCGGATGCTG CTCAAGCTAA TAAGAACCAT 
CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA 
CAGATGTCAC ACAGATTAGA GCCTCGCAGG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001094112.1
CDS	72..3107
Translation	MVAAHAAHSPSSAEWIACLDKRPLERSSEDVDIIFTRLKGVKAFERFHPNLLRQICLCSYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRENSELLRIEQEDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPAHTITKVPSEKILRAGKILRIAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQVLLEDGVLNHVDQERHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTADDLEIIYDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEPSLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGTEQERMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMVAFKEQLAELEKTVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYTTIRVPVAASVKEVISAVADKLGSGEGLIIVKMNSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP

Target ORF information:

RefSeq Version	NM_001100642.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100642.1

ORF Insert Sequence:

ATGGTTGCTG CGCACGCTGC ACACTCTCCG TCCTCGGCCG AGTGGATCGC CTGCCTGGAT 
AAAAGGCCGT TGGAGCGATC TAGTGAAGAT GTGGACATAA TTTTCACGCG GCTGAAAGGA 
GTTAAAGCTT TTGAGAGATT TCACCCAAAC CTCCTTCGTC AGATTTGTTT ATGCAGTTAC 
TATGAGAACC TGGAGAAAGG AATCACACTG TTTCGACAAG GGGACATTGG AACCAACTGG 
TATGCTGTCC TAGCTGGGTC TTTGGATGTT AAAGTGTCTG AGACCAGCAG TCACCAGGAT 
GCGGTGACCA TCTGTACCCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTCGACAAC 
ACACCTCGTC ATGCAACCAT TGTTACCAGA GAGAACAGTG AGCTCCTCCG TATCGAGCAG 
GAAGACTTTA AGGCACTATG GGAGAAATAC CGACAGTATA TGGCCGGACT TCTGGCTCCT 
CCCTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCGGACAA GGAGAATACA 
CCTCTCATTG AACCTCACGT TCCTCTCCGC CCTGCTCACA CCATTACCAA GGTCCCTTCA 
GAGAAGATCC TCAGAGCTGG AAAAATTTTG CGAATTGCCA TTCTCTCTCG AGCACCCCAC 
ATGATAAGAG ACAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT TGGGACTGAG 
CTGGTAGACT GGATGATACA GCAGACGTCC TGTGTTCACT CCCGGACTCA AGCTGTTGGC 
ATGTGGCAAG TCTTGCTGGA AGATGGCGTC CTCAACCACG TGGACCAGGA GCGCCATTTC 
CAAGATAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ATGAGGATGC CCCTTTGCCT 
ACTGAGGAGG AGAAGAAGGA GTGCGATGAA GAGCTCCAGG ACACCATGCT GCTGCTGTCA 
CAAATGGGCC CTGACGCCCA CATGAGAATG ATCCTGCGAA AACCACCTGG CCAGAGGACT 
GCAGATGACC TAGAGATTAT CTACGACGAA CTCCTTCATA TTAAAGCCTT ATCCCATCTT 
TCTACCACAG TGAAACGGGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA 
GGAACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATCAT TCTGAAAGGA 
TCCGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC 
TTTGGCAAGT TAGCTCTGGT GAACGATGCT CCGAGAGCTG CATCCATTGT TCTTCGGGAA 
GATAATTGTC ACTTCCTAAG AGTTGACAAG GAAGACTTCA ATCGGATTCT GAGGGACGTT 
GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTACT GGAGAAGGTC 
CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG 
ATGTCAGGAA CACCTGAAAA GATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA 
TCTTTGAATG AAGCAACAGA TTCAGTTTTA AATGACTTCG TTATGATGCA CTGTGTTTTT 
ATGCCAAACA CCCAGCTTTG CCCCGCCCTC GTGGCCCATT ACCACGCACA GCCTTCTCAA 
GGTACAGAAC AGGAGAGAAT GGATTACGCC CTCAACAACA AGAGGCGGGT CATCCGCCTG 
GTTCTGCAAT GGGCAGCCAT GTATGGTGAT CTTCTCCAAG AAGACGACGT GGCCATGGCT 
TTCCTGGAGG AGTTCTATGT GTCTGTATCA GATGATGCAC GGATGATGGT CGCCTTCAAG 
GAGCAGCTGG CTGAGCTGGA GAAGACTGTC AAACAGATCT CAGAAGACGC AAAAGCTCCA 
CAGAAGAAGC ACAAGGTGCT TTTGCAGCAG TTTAATACAG GTGACGAGAG GGCCCAGAAG 
CGTCAGCCGA TTCGTGGCTC TGATGAGGTT TTGTTCAAGG TCTACTGCAT TGACCACACC 
TATACCACCA TCCGAGTGCC TGTAGCCGCC TCGGTGAAGG AAGTCATCAG TGCAGTAGCA 
GACAAACTTG GCTCTGGGGA AGGCCTGATC ATTGTCAAGA TGAACTCTGG AGGAGAAAAG 
GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG 
TTTGCCTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCG 
ACCACTGGGA CAGTGGGGAC GTTTGAACTG ATGAGCTCGA AAGACTTGGC GTACCAGATG 
ACAACGTATG ACTGGGAACT CTTCAACTGT GTGCACGAGC TGGAGCTAAT CTACCACACA 
TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT 
AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCT CAGCAAACGT 
GTTCAGCTTT TGAAAAAATT TATCAAGATA GCGGCTCACT GCAAGGAGTA CAAAAACCTG 
AATTCCTTCT TCGCCATCGT CATGGGGCTC AGTAACGTGG CTGTGAGCCG CCTGGCATTA 
ACATGGGAGA AACTGCCGAG CAAGTTTAAG AAGTTCTATG CGGAGTTTGA GAGCTTAATG 
GATCCTTCCA GAAATCACAG GGCGTACAGG CTGACAGCAG CTAAACTGGA GCCCCCCCTC 
ATCCCTTTCA TGCCCTTGCT TATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA 
TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACG 
GTGCGCTACT ACAGGAGCCA GCCATTCAAT CCGGATGCTG CTCAAGCTAA TAAGAACCAT 
CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA 
CAGATGTCAC ACAGATTAGA GCCTCGCAGG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef4 ( XM_001060956.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001060956.2 Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa15670
Clone ID Related Accession (Same CDS sequence)	NM_001100642.1 , XM_001060956.5 , XM_017592164.1
Accession Version	XM_001060956.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) and transcript sequence (AB031229.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_001060956.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 141 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTTGCTG CGCACGCTGC ACACTCTCCG TCCTCGGCCG AGTGGATCGC CTGCCTGGAT 
AAAAGGCCGT TGGAGCGATC TAGTGAAGAT GTGGACATAA TTTTCACGCG GCTGAAAGGA 
GTTAAAGCTT TTGAGAGATT TCACCCAAAC CTCCTTCGTC AGATTTGTTT ATGCAGTTAC 
TATGAGAACC TGGAGAAAGG AATCACACTG TTTCGACAAG GGGACATTGG AACCAACTGG 
TATGCTGTCC TAGCTGGGTC TTTGGATGTT AAAGTGTCTG AGACCAGCAG TCACCAGGAT 
GCGGTGACCA TCTGTACCCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTCGACAAC 
ACACCTCGTC ATGCAACCAT TGTTACCAGA GAGAACAGTG AGCTCCTCCG TATCGAGCAG 
GAAGACTTTA AGGCACTATG GGAGAAATAC CGACAGTATA TGGCCGGACT TCTGGCTCCT 
CCCTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCGGACAA GGAGAATACA 
CCTCTCATTG AACCTCACGT TCCTCTCCGC CCTGCTCACA CCATTACCAA GGTCCCTTCA 
GAGAAGATCC TCAGAGCTGG AAAAATTTTG CGAATTGCCA TTCTCTCTCG AGCACCCCAC 
ATGATAAGAG ACAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT TGGGACTGAG 
CTGGTAGACT GGATGATACA GCAGACGTCC TGTGTTCACT CCCGGACTCA AGCTGTTGGC 
ATGTGGCAAG TCTTGCTGGA AGATGGCGTC CTCAACCACG TGGACCAGGA GCGCCATTTC 
CAAGATAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ATGAGGATGC CCCTTTGCCT 
ACTGAGGAGG AGAAGAAGGA GTGCGATGAA GAGCTCCAGG ACACCATGCT GCTGCTGTCA 
CAAATGGGCC CTGACGCCCA CATGAGAATG ATCCTGCGAA AACCACCTGG CCAGAGGACT 
GCAGATGACC TAGAGATTAT CTACGACGAA CTCCTTCATA TTAAAGCCTT ATCCCATCTT 
TCTACCACAG TGAAACGGGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA 
GGAACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATCAT TCTGAAAGGA 
TCCGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC 
TTTGGCAAGT TAGCTCTGGT GAACGATGCT CCGAGAGCTG CATCCATTGT TCTTCGGGAA 
GATAATTGTC ACTTCCTAAG AGTTGACAAG GAAGACTTCA ATCGGATTCT GAGGGACGTT 
GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTACT GGAGAAGGTC 
CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG 
ATGTCAGGAA CACCTGAAAA GATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA 
TCTTTGAATG AAGCAACAGA TTCAGTTTTA AATGACTTCG TTATGATGCA CTGTGTTTTT 
ATGCCAAACA CCCAGCTTTG CCCCGCCCTC GTGGCCCATT ACCACGCACA GCCTTCTCAA 
GGTACAGAAC AGGAGAGAAT GGATTACGCC CTCAACAACA AGAGGCGGGT CATCCGCCTG 
GTTCTGCAAT GGGCAGCCAT GTATGGTGAT CTTCTCCAAG AAGACGACGT GGCCATGGCT 
TTCCTGGAGG AGTTCTATGT GTCTGTATCA GATGATGCAC GGATGATGGT CGCCTTCAAG 
GAGCAGCTGG CTGAGCTGGA GAAGACTGTC AAACAGATCT CAGAAGACGC AAAAGCTCCA 
CAGAAGAAGC ACAAGGTGCT TTTGCAGCAG TTTAATACAG GTGACGAGAG GGCCCAGAAG 
CGTCAGCCGA TTCGTGGCTC TGATGAGGTT TTGTTCAAGG TCTACTGCAT TGACCACACC 
TATACCACCA TCCGAGTGCC TGTAGCCGCC TCGGTGAAGG AAGTCATCAG TGCAGTAGCA 
GACAAACTTG GCTCTGGGGA AGGCCTGATC ATTGTCAAGA TGAACTCTGG AGGAGAAAAG 
GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG 
TTTGCCTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCG 
ACCACTGGGA CAGTGGGGAC GTTTGAACTG ATGAGCTCGA AAGACTTGGC GTACCAGATG 
ACAACGTATG ACTGGGAACT CTTCAACTGT GTGCACGAGC TGGAGCTAAT CTACCACACA 
TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT 
AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCT CAGCAAACGT 
GTTCAGCTTT TGAAAAAATT TATCAAGATA GCGGCTCACT GCAAGGAGTA CAAAAACCTG 
AATTCCTTCT TCGCCATCGT CATGGGGCTC AGTAACGTGG CTGTGAGCCG CCTGGCATTA 
ACATGGGAGA AACTGCCGAG CAAGTTTAAG AAGTTCTATG CGGAGTTTGA GAGCTTAATG 
GATCCTTCCA GAAATCACAG GGCGTACAGG CTGACAGCAG CTAAACTGGA GCCCCCCCTC 
ATCCCTTTCA TGCCCTTGCT TATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA 
TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACG 
GTGCGCTACT ACAGGAGCCA GCCATTCAAT CCGGATGCTG CTCAAGCTAA TAAGAACCAT 
CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA 
CAGATGTCAC ACAGATTAGA GCCTCGCAGG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001060956.2
CDS	213..3248
Translation	MVAAHAAHSPSSAEWIACLDKRPLERSSEDVDIIFTRLKGVKAFERFHPNLLRQICLCSYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRENSELLRIEQEDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPAHTITKVPSEKILRAGKILRIAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQVLLEDGVLNHVDQERHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTADDLEIIYDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEPSLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGTEQERMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMVAFKEQLAELEKTVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYTTIRVPVAASVKEVISAVADKLGSGEGLIIVKMNSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP

Target ORF information:

RefSeq Version	XM_001060956.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001060956.5

ORF Insert Sequence:

ATGGTTGCTG CGCACGCTGC ACACTCTCCG TCCTCGGCCG AGTGGATCGC CTGCCTGGAT 
AAAAGGCCGT TGGAGCGATC TAGTGAAGAT GTGGACATAA TTTTCACGCG GCTGAAAGGA 
GTTAAAGCTT TTGAGAGATT TCACCCAAAC CTCCTTCGTC AGATTTGTTT ATGCAGTTAC 
TATGAGAACC TGGAGAAAGG AATCACACTG TTTCGACAAG GGGACATTGG AACCAACTGG 
TATGCTGTCC TAGCTGGGTC TTTGGATGTT AAAGTGTCTG AGACCAGCAG TCACCAGGAT 
GCGGTGACCA TCTGTACCCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTCGACAAC 
ACACCTCGTC ATGCAACCAT TGTTACCAGA GAGAACAGTG AGCTCCTCCG TATCGAGCAG 
GAAGACTTTA AGGCACTATG GGAGAAATAC CGACAGTATA TGGCCGGACT TCTGGCTCCT 
CCCTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCGGACAA GGAGAATACA 
CCTCTCATTG AACCTCACGT TCCTCTCCGC CCTGCTCACA CCATTACCAA GGTCCCTTCA 
GAGAAGATCC TCAGAGCTGG AAAAATTTTG CGAATTGCCA TTCTCTCTCG AGCACCCCAC 
ATGATAAGAG ACAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT TGGGACTGAG 
CTGGTAGACT GGATGATACA GCAGACGTCC TGTGTTCACT CCCGGACTCA AGCTGTTGGC 
ATGTGGCAAG TCTTGCTGGA AGATGGCGTC CTCAACCACG TGGACCAGGA GCGCCATTTC 
CAAGATAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ATGAGGATGC CCCTTTGCCT 
ACTGAGGAGG AGAAGAAGGA GTGCGATGAA GAGCTCCAGG ACACCATGCT GCTGCTGTCA 
CAAATGGGCC CTGACGCCCA CATGAGAATG ATCCTGCGAA AACCACCTGG CCAGAGGACT 
GCAGATGACC TAGAGATTAT CTACGACGAA CTCCTTCATA TTAAAGCCTT ATCCCATCTT 
TCTACCACAG TGAAACGGGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA 
GGAACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATCAT TCTGAAAGGA 
TCCGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC 
TTTGGCAAGT TAGCTCTGGT GAACGATGCT CCGAGAGCTG CATCCATTGT TCTTCGGGAA 
GATAATTGTC ACTTCCTAAG AGTTGACAAG GAAGACTTCA ATCGGATTCT GAGGGACGTT 
GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTACT GGAGAAGGTC 
CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG 
ATGTCAGGAA CACCTGAAAA GATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA 
TCTTTGAATG AAGCAACAGA TTCAGTTTTA AATGACTTCG TTATGATGCA CTGTGTTTTT 
ATGCCAAACA CCCAGCTTTG CCCCGCCCTC GTGGCCCATT ACCACGCACA GCCTTCTCAA 
GGTACAGAAC AGGAGAGAAT GGATTACGCC CTCAACAACA AGAGGCGGGT CATCCGCCTG 
GTTCTGCAAT GGGCAGCCAT GTATGGTGAT CTTCTCCAAG AAGACGACGT GGCCATGGCT 
TTCCTGGAGG AGTTCTATGT GTCTGTATCA GATGATGCAC GGATGATGGT CGCCTTCAAG 
GAGCAGCTGG CTGAGCTGGA GAAGACTGTC AAACAGATCT CAGAAGACGC AAAAGCTCCA 
CAGAAGAAGC ACAAGGTGCT TTTGCAGCAG TTTAATACAG GTGACGAGAG GGCCCAGAAG 
CGTCAGCCGA TTCGTGGCTC TGATGAGGTT TTGTTCAAGG TCTACTGCAT TGACCACACC 
TATACCACCA TCCGAGTGCC TGTAGCCGCC TCGGTGAAGG AAGTCATCAG TGCAGTAGCA 
GACAAACTTG GCTCTGGGGA AGGCCTGATC ATTGTCAAGA TGAACTCTGG AGGAGAAAAG 
GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG 
TTTGCCTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCG 
ACCACTGGGA CAGTGGGGAC GTTTGAACTG ATGAGCTCGA AAGACTTGGC GTACCAGATG 
ACAACGTATG ACTGGGAACT CTTCAACTGT GTGCACGAGC TGGAGCTAAT CTACCACACA 
TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT 
AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCT CAGCAAACGT 
GTTCAGCTTT TGAAAAAATT TATCAAGATA GCGGCTCACT GCAAGGAGTA CAAAAACCTG 
AATTCCTTCT TCGCCATCGT CATGGGGCTC AGTAACGTGG CTGTGAGCCG CCTGGCATTA 
ACATGGGAGA AACTGCCGAG CAAGTTTAAG AAGTTCTATG CGGAGTTTGA GAGCTTAATG 
GATCCTTCCA GAAATCACAG GGCGTACAGG CTGACAGCAG CTAAACTGGA GCCCCCCCTC 
ATCCCTTTCA TGCCCTTGCT TATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA 
TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACG 
GTGCGCTACT ACAGGAGCCA GCCATTCAAT CCGGATGCTG CTCAAGCTAA TAAGAACCAT 
CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA 
CAGATGTCAC ACAGATTAGA GCCTCGCAGG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rapgef4 ( XM_017592164.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447653.1 Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), transcript variant X1, mRNA.

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CloneID	ORa15670
Clone ID Related Accession (Same CDS sequence)	NM_001100642.1 , XM_001060956.5 , XM_017592164.1
Accession Version	XM_017592164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	rap guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) and transcript sequence (AB031229.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 174 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTTGCTG CGCACGCTGC ACACTCTCCG TCCTCGGCCG AGTGGATCGC CTGCCTGGAT 
AAAAGGCCGT TGGAGCGATC TAGTGAAGAT GTGGACATAA TTTTCACGCG GCTGAAAGGA 
GTTAAAGCTT TTGAGAGATT TCACCCAAAC CTCCTTCGTC AGATTTGTTT ATGCAGTTAC 
TATGAGAACC TGGAGAAAGG AATCACACTG TTTCGACAAG GGGACATTGG AACCAACTGG 
TATGCTGTCC TAGCTGGGTC TTTGGATGTT AAAGTGTCTG AGACCAGCAG TCACCAGGAT 
GCGGTGACCA TCTGTACCCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTCGACAAC 
ACACCTCGTC ATGCAACCAT TGTTACCAGA GAGAACAGTG AGCTCCTCCG TATCGAGCAG 
GAAGACTTTA AGGCACTATG GGAGAAATAC CGACAGTATA TGGCCGGACT TCTGGCTCCT 
CCCTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCGGACAA GGAGAATACA 
CCTCTCATTG AACCTCACGT TCCTCTCCGC CCTGCTCACA CCATTACCAA GGTCCCTTCA 
GAGAAGATCC TCAGAGCTGG AAAAATTTTG CGAATTGCCA TTCTCTCTCG AGCACCCCAC 
ATGATAAGAG ACAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT TGGGACTGAG 
CTGGTAGACT GGATGATACA GCAGACGTCC TGTGTTCACT CCCGGACTCA AGCTGTTGGC 
ATGTGGCAAG TCTTGCTGGA AGATGGCGTC CTCAACCACG TGGACCAGGA GCGCCATTTC 
CAAGATAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ATGAGGATGC CCCTTTGCCT 
ACTGAGGAGG AGAAGAAGGA GTGCGATGAA GAGCTCCAGG ACACCATGCT GCTGCTGTCA 
CAAATGGGCC CTGACGCCCA CATGAGAATG ATCCTGCGAA AACCACCTGG CCAGAGGACT 
GCAGATGACC TAGAGATTAT CTACGACGAA CTCCTTCATA TTAAAGCCTT ATCCCATCTT 
TCTACCACAG TGAAACGGGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA 
GGAACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATCAT TCTGAAAGGA 
TCCGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC 
TTTGGCAAGT TAGCTCTGGT GAACGATGCT CCGAGAGCTG CATCCATTGT TCTTCGGGAA 
GATAATTGTC ACTTCCTAAG AGTTGACAAG GAAGACTTCA ATCGGATTCT GAGGGACGTT 
GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTACT GGAGAAGGTC 
CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG 
ATGTCAGGAA CACCTGAAAA GATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA 
TCTTTGAATG AAGCAACAGA TTCAGTTTTA AATGACTTCG TTATGATGCA CTGTGTTTTT 
ATGCCAAACA CCCAGCTTTG CCCCGCCCTC GTGGCCCATT ACCACGCACA GCCTTCTCAA 
GGTACAGAAC AGGAGAGAAT GGATTACGCC CTCAACAACA AGAGGCGGGT CATCCGCCTG 
GTTCTGCAAT GGGCAGCCAT GTATGGTGAT CTTCTCCAAG AAGACGACGT GGCCATGGCT 
TTCCTGGAGG AGTTCTATGT GTCTGTATCA GATGATGCAC GGATGATGGT CGCCTTCAAG 
GAGCAGCTGG CTGAGCTGGA GAAGACTGTC AAACAGATCT CAGAAGACGC AAAAGCTCCA 
CAGAAGAAGC ACAAGGTGCT TTTGCAGCAG TTTAATACAG GTGACGAGAG GGCCCAGAAG 
CGTCAGCCGA TTCGTGGCTC TGATGAGGTT TTGTTCAAGG TCTACTGCAT TGACCACACC 
TATACCACCA TCCGAGTGCC TGTAGCCGCC TCGGTGAAGG AAGTCATCAG TGCAGTAGCA 
GACAAACTTG GCTCTGGGGA AGGCCTGATC ATTGTCAAGA TGAACTCTGG AGGAGAAAAG 
GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG 
TTTGCCTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCG 
ACCACTGGGA CAGTGGGGAC GTTTGAACTG ATGAGCTCGA AAGACTTGGC GTACCAGATG 
ACAACGTATG ACTGGGAACT CTTCAACTGT GTGCACGAGC TGGAGCTAAT CTACCACACA 
TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT 
AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCT CAGCAAACGT 
GTTCAGCTTT TGAAAAAATT TATCAAGATA GCGGCTCACT GCAAGGAGTA CAAAAACCTG 
AATTCCTTCT TCGCCATCGT CATGGGGCTC AGTAACGTGG CTGTGAGCCG CCTGGCATTA 
ACATGGGAGA AACTGCCGAG CAAGTTTAAG AAGTTCTATG CGGAGTTTGA GAGCTTAATG 
GATCCTTCCA GAAATCACAG GGCGTACAGG CTGACAGCAG CTAAACTGGA GCCCCCCCTC 
ATCCCTTTCA TGCCCTTGCT TATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA 
TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACG 
GTGCGCTACT ACAGGAGCCA GCCATTCAAT CCGGATGCTG CTCAAGCTAA TAAGAACCAT 
CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA 
CAGATGTCAC ACAGATTAGA GCCTCGCAGG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447653.1
CDS	139..3174
Translation	MVAAHAAHSPSSAEWIACLDKRPLERSSEDVDIIFTRLKGVKAFERFHPNLLRQICLCSYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDNTPRHATIVTRENSELLRIEQEDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPAHTITKVPSEKILRAGKILRIAILSRAPHMIRDRKYHLKTYRQCCVGTELVDWMIQQTSCVHSRTQAVGMWQVLLEDGVLNHVDQERHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTADDLEIIYDELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEPSLNEATDSVLNDFVMMHCVFMPNTQLCPALVAHYHAQPSQGTEQERMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVAMAFLEEFYVSVSDDARMMVAFKEQLAELEKTVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCIDHTYTTIRVPVAASVKEVISAVADKLGSGEGLIIVKMNSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTTGTVGTFELMSSKDLAYQMTTYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTAAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP

Target ORF information:

RefSeq Version	XM_017592164.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rap guanine nucleotide exchange factor 4 (Rapgef4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592164.1

ORF Insert Sequence:

ATGGTTGCTG CGCACGCTGC ACACTCTCCG TCCTCGGCCG AGTGGATCGC CTGCCTGGAT 
AAAAGGCCGT TGGAGCGATC TAGTGAAGAT GTGGACATAA TTTTCACGCG GCTGAAAGGA 
GTTAAAGCTT TTGAGAGATT TCACCCAAAC CTCCTTCGTC AGATTTGTTT ATGCAGTTAC 
TATGAGAACC TGGAGAAAGG AATCACACTG TTTCGACAAG GGGACATTGG AACCAACTGG 
TATGCTGTCC TAGCTGGGTC TTTGGATGTT AAAGTGTCTG AGACCAGCAG TCACCAGGAT 
GCGGTGACCA TCTGTACCCT GGGAATTGGG ACAGCCTTTG GAGAGTCCAT TCTCGACAAC 
ACACCTCGTC ATGCAACCAT TGTTACCAGA GAGAACAGTG AGCTCCTCCG TATCGAGCAG 
GAAGACTTTA AGGCACTATG GGAGAAATAC CGACAGTATA TGGCCGGACT TCTGGCTCCT 
CCCTATGGTG TTATGGAAAC GGGCTCTAAC AATGACAGGA TTCCGGACAA GGAGAATACA 
CCTCTCATTG AACCTCACGT TCCTCTCCGC CCTGCTCACA CCATTACCAA GGTCCCTTCA 
GAGAAGATCC TCAGAGCTGG AAAAATTTTG CGAATTGCCA TTCTCTCTCG AGCACCCCAC 
ATGATAAGAG ACAGAAAATA CCACCTAAAG ACATACAGAC AATGCTGTGT TGGGACTGAG 
CTGGTAGACT GGATGATACA GCAGACGTCC TGTGTTCACT CCCGGACTCA AGCTGTTGGC 
ATGTGGCAAG TCTTGCTGGA AGATGGCGTC CTCAACCACG TGGACCAGGA GCGCCATTTC 
CAAGATAAAT ATTTATTTTA TCGATTTCTG GATGACGAGC ATGAGGATGC CCCTTTGCCT 
ACTGAGGAGG AGAAGAAGGA GTGCGATGAA GAGCTCCAGG ACACCATGCT GCTGCTGTCA 
CAAATGGGCC CTGACGCCCA CATGAGAATG ATCCTGCGAA AACCACCTGG CCAGAGGACT 
GCAGATGACC TAGAGATTAT CTACGACGAA CTCCTTCATA TTAAAGCCTT ATCCCATCTT 
TCTACCACAG TGAAACGGGA GTTAGCAGGT GTTCTCATTT TTGAGTCTCA CGCCAAAGGA 
GGAACTGTGT TGTTTAACCA GGGGGAAGAA GGTACCTCCT GGTACATCAT TCTGAAAGGA 
TCCGTGAATG TAGTCATTTA TGGCAAGGGT GTGGTCTGCA CCCTGCATGA GGGAGATGAC 
TTTGGCAAGT TAGCTCTGGT GAACGATGCT CCGAGAGCTG CATCCATTGT TCTTCGGGAA 
GATAATTGTC ACTTCCTAAG AGTTGACAAG GAAGACTTCA ATCGGATTCT GAGGGACGTT 
GAGGCGAATA CAGTCAGACT TAAAGAACAT GACCAAGATG TCTTGGTACT GGAGAAGGTC 
CCAGCAGGGA ACAGAGCTTC TAATCAAGGA AACTCACAGC CTCAGCAAAA GTATACTGTG 
ATGTCAGGAA CACCTGAAAA GATTTTAGAG CATTTTCTAG AAACAATACG CCTTGAGCCA 
TCTTTGAATG AAGCAACAGA TTCAGTTTTA AATGACTTCG TTATGATGCA CTGTGTTTTT 
ATGCCAAACA CCCAGCTTTG CCCCGCCCTC GTGGCCCATT ACCACGCACA GCCTTCTCAA 
GGTACAGAAC AGGAGAGAAT GGATTACGCC CTCAACAACA AGAGGCGGGT CATCCGCCTG 
GTTCTGCAAT GGGCAGCCAT GTATGGTGAT CTTCTCCAAG AAGACGACGT GGCCATGGCT 
TTCCTGGAGG AGTTCTATGT GTCTGTATCA GATGATGCAC GGATGATGGT CGCCTTCAAG 
GAGCAGCTGG CTGAGCTGGA GAAGACTGTC AAACAGATCT CAGAAGACGC AAAAGCTCCA 
CAGAAGAAGC ACAAGGTGCT TTTGCAGCAG TTTAATACAG GTGACGAGAG GGCCCAGAAG 
CGTCAGCCGA TTCGTGGCTC TGATGAGGTT TTGTTCAAGG TCTACTGCAT TGACCACACC 
TATACCACCA TCCGAGTGCC TGTAGCCGCC TCGGTGAAGG AAGTCATCAG TGCAGTAGCA 
GACAAACTTG GCTCTGGGGA AGGCCTGATC ATTGTCAAGA TGAACTCTGG AGGAGAAAAG 
GTGGTGCTCA AACCTAATGA TGTTTCAGTA TTTACGACGC TCACCATTAA TGGACGCCTG 
TTTGCCTGCC CGCGAGAGCA ATTCGACTCA CTGACTCCCT TGCCAGAACA GGAGGGCCCG 
ACCACTGGGA CAGTGGGGAC GTTTGAACTG ATGAGCTCGA AAGACTTGGC GTACCAGATG 
ACAACGTATG ACTGGGAACT CTTCAACTGT GTGCACGAGC TGGAGCTAAT CTACCACACA 
TTTGGAAGGC ATAATTTTAA AAAGACCACA GCAAACTTGG ATTTGTTCCT GAGGAGATTT 
AATGAAATTC AGTTTTGGGT TGTCACTGAG ATCTGCCTTT GTTCCCAGCT CAGCAAACGT 
GTTCAGCTTT TGAAAAAATT TATCAAGATA GCGGCTCACT GCAAGGAGTA CAAAAACCTG 
AATTCCTTCT TCGCCATCGT CATGGGGCTC AGTAACGTGG CTGTGAGCCG CCTGGCATTA 
ACATGGGAGA AACTGCCGAG CAAGTTTAAG AAGTTCTATG CGGAGTTTGA GAGCTTAATG 
GATCCTTCCA GAAATCACAG GGCGTACAGG CTGACAGCAG CTAAACTGGA GCCCCCCCTC 
ATCCCTTTCA TGCCCTTGCT TATTAAAGAT ATGACATTTA CTCATGAGGG GAACAAGACA 
TTCATTGACA ATCTAGTAAA CTTTGAAAAA ATGCGCATGA TTGCAAATAC TGCCAGAACG 
GTGCGCTACT ACAGGAGCCA GCCATTCAAT CCGGATGCTG CTCAAGCTAA TAAGAACCAT 
CAGGATGTCC GGAGTTATGT ACGGCAATTA AATGTGATTG ACAACCAGAG AACTTTATCA 
CAGATGTCAC ACAGATTAGA GCCTCGCAGG CCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Guanine nucleotide exchange factor Epac2-dependent activation of the GTP-binding protein Rap2A mediates cAMP-dependent growth arrest in neuroendocrine cells.
The Journal of biological chemistry292(29)12220-12231(2017 07)
Emery AC,Xu W,Eiden MV,Eiden LE

Exchange proteins directly activated by cAMP induce the proliferation of rat anterior pituitary GH3 cells via the activation of extracellular signal-regulated kinase.
Biochemical and biophysical research communications485(2)355-359(2017 04)
Sun W,Jiao W,Huang Y,Li R,Zhang Z,Wang J,Lei T

EPAC activation inhibits acetaldehyde-induced activation and proliferation of hepatic stellate cell via Rap1.
Canadian journal of physiology and pharmacology94(5)498-507(2016 May)
Yang Y,Yang F,Wu X,Lv X,Li J

Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus.
The Journal of neuroscience : the official journal of the Society for Neuroscience35(16)6544-53(2015 Apr)
Fernandes HB,Riordan S,Nomura T,Remmers CL,Kraniotis S,Marshall JJ,Kukreja L,Vassar R,Contractor A

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Rapgef4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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