Smo cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smo
Entrez Gene ID	25273
Full Name	smoothened, frizzled class receptor
Synonyms	Smoh
General protein information	Preferred Names smoothened, frizzled class receptor Names smoothened homolog smoothened, frizzled family receptor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q22
Summary	may act as a signaling component of the Patched (Ptch) signaling pathway [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012807.1	NP_036939.1	smoothened homolog precursor

Pathways from BioSystems

Homologies

	SMO	XP_001093150.1
Mus musculus (house mouse)	Smo	NP_795970.3
Gallus gallus (chicken)	SMO	XP_414970.4
Danio rerio (zebrafish)	smo	NP_571102.1
Xenopus tropicalis (tropical clawed frog)	smo	XP_002931911.2
Pan troglodytes (chimpanzee)	SMO	XP_519371.3
Canis lupus familiaris (dog)	SMO	XP_005628462.1
Anopheles gambiae (African malaria mosquito)	GPRSMO	XP_314720.1
Bos taurus (cattle)	SMO	NP_001179149.1
Homo sapiens (human)	SMO	NP_005622.1
Rattus norvegicus (Norway rat)	Smo	NP_036939.1
Drosophila melanogaster (fruit fly)	smo	NP_523443.1

Gene Ontology
Bibliography

Related articles in PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
Badgandi HB, Hwang SH, Shimada IS, Loriot E, Mukhopadhyay S
The Journal of cell biology216(3)743-760(2017 Mar)

Intrinsic facilitation of adult peripheral nerve regeneration by the Sonic hedgehog morphogen.
Martinez JA, Kobayashi M, Krishnan A, Webber C, Christie K, Guo G, Singh V, Zochodne DW
Experimental neurology271493-505(2015 Sep)

FGFR3 Deficiency Causes Multiple Chondroma-like Lesions by Upregulating Hedgehog Signaling.
Zhou S, Xie Y, Tang J, Huang J, Huang Q, Xu W, Wang Z, Luo F, Wang Q, Chen H, Du X, Shen Y, Chen D, Chen L
PLoS genetics11(6)e1005214(2015 Jun)

Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5.
Chong YC, Mann RK, Zhao C, Kato M, Beachy PA
Genes & development29(3)262-76(2015 Feb)

Expression of hedgehog signal pathway in articular cartilage is associated with the severity of cartilage damage in rats with adjuvant-induced arthritis.
Li R, Cai L, Hu CM, Wu TN, Li J
Journal of inflammation (London, England)1224(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study characterizes, for the first time, the transcriptional response of cardiomyocytes to Shh and establishes a critical role for Smo coupling to Gi in Hh signaling in the normal and ischemic myocardium
Title: Gi proteins mediate activation of the canonical hedgehog pathway in the myocardium.

The expression of Shh, Smo and Gli1 at the protein and mRNA level in hepatocytes of rats with chronic fluorosis can be increased by fluoride and may be inhibited by cyclopamine.
Title: Protein and mRNA expression of Shh, Smo and Gli1 and inhibition by cyclopamine in hepatocytes of rats with chronic fluorosis.

Ptch1 and Smo are predominantly localized in the postsynaptic side of the synapses, a distribution pattern similar to that found in hippocampal neurons
Title: Subcellular distribution of patched and smoothened in the cerebellar neurons.

In young rat hippocampal neurons, Smo is present in the processes and growth cones. In mature rat hippocampal neurons, Smo is located predominately in dendrites, dendritic spines, and postsynaptic sites.
Title: Subcellular localization of Patched and Smoothened, the receptors for Sonic hedgehog signaling, in the hippocampal neuron.

Shh and Smo are upregulated in a time-dependent manner in retinas exposed to ocular hypertension, and Shh has neuroprotective effects on damaged RGCs in a rat chronic hypertension model.
Title: Neuroprotective effect of upregulated sonic Hedgehog in retinal ganglion cells following chronic ocular hypertension.

The following Smo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13771	NM_012807.1 Latest version!	Rattus norvegicus smoothened, frizzled class receptor (Smo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$468.30 $669.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smo ( NM_012807.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036939.1 Rattus norvegicus smoothened, frizzled class receptor (Smo), mRNA.

Price & Availability↑

CloneID	ORa13771
Clone ID Related Accession (Same CDS sequence)	NM_012807.1
Accession Version	NM_012807.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Rattus norvegicus(Norway rat)
Product	smoothened homolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U84402.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U84402.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCTG GCCGCCCCGT GCGTGGGCCC GAGCTGGCGC CCCGGAGGCT GCTGCAGTTG 
CTGCTGCTGG TACTGCTTGG GGGCCGGGGC CGGGGGGCGG CCTTGAGCGG GAACGTGACC 
GGGCCTGGGC CTCGCAGTGC CGGCGGGAGC GCGAGGAGGA ACGCGCCGGT GACCAGCCCT 
CCGCCGCCGC TGCTGAGCCA CTGCGGCCGG GCCGCCCACT GCGAGCCTTT GCGCTACAAC 
GTGTGCCTGG GCTCCGCGCT GCCCTACGGA GCCACCACCA CGCTGCTGGC TGGGGACTCG 
GACTCGCAGG AGGAAGCGCA CAGCAAGCTC GTGCTCTGGT CCGGCCTCCG GAATGCTCCC 
CGATGCTGGG CAGTGATCCA GCCCCTGCTG TGTGCTGTCT ACATGCCCAA GTGTGAAAAT 
GACCGAGTGG AGTTGCCCAG CCGTACCCTC TGCCAGGCCA CCCGAGGCCC CTGTGCCATT 
GTGGAGCGGG AACGAGGGTG GCCTGACTTT CTGCGTTGCA CGCCGGACCA CTTCCCTGAA 
GGCTGTCCAA ACGAGGTACA AAACATCAAG TTCAACAGTT CAGGCCAATG TGAAGCACCC 
TTGGTGAGGA CAGACAACCC CAAGAGCTGG TACGAGGACG TGGAGGGCTG TGGGATCCAG 
TGCCAGAACC CGCTGTTCAC CGAGGCTGAG CACCAGGACA TGCACAGTTA CATCGCAGCC 
TTCGGGGCGG TCACCGGCCT CTGTACACTC TTCACCCTGG CCACCTTTGT GGCTGACTGG 
CGGAACTCCA ATCGCTACCC TGCGGTTATT CTCTTCTATG TCAATGCGTG TTTCTTTGTG 
GGCAGCATTG GCTGGCTGGC CCAGTTCATG GATGGTGCCC GCCGGGAGAT TGTTTGCCGA 
GCAGATGGCA CCATGAGATT TGGGGAGCCC ACCTCCAGCG AGACCCTATC CTGTGTCATC 
ATCTTTGTCA TCGTGTACTA TGCCTTGATG GCTGGAGTAG TGTGGTTCGT GGTCCTCACC 
TATGCCTGGC ACACCTCCTT CAAAGCCCTG GGCACCACTT ACCAGCCTCT CTCGGGCAAG 
ACATCCTATT TCCACCTGCT CACGTGGTCA CTCCCCTTCG TCCTCACTGT GGCAATCCTT 
GCTGTGGCTC AGGTAGATGG GGACTCCGTG AGTGGCATCT GCTTTGTAGG CTACAAGAAC 
TATCGGTACC GTGCTGGCTT TGTACTTGCC CCAATTGGCC TGGTGCTTAT TGTGGGAGGC 
TACTTCCTCA TCCGAGGGGT CATGACTCTG TTCTCCATCA AGAGCAACCA CCCTGGGCTT 
CTGAGTGAGA AGGCAGCCAG CAAGATCAAT GAGACCATGC TGCGCCTGGG CATTTTTGGC 
TTCCTCGCCT TTGGCTTCGT GCTCATCACC TTCAGCTGCC ACTTCTATGA CTTCTTCAAC 
CAGGCTGAGT GGGAGCGTAG CTTCCGGGAC TATGTGCTAT GCCAAGCCAA TGTGACCATT 
GGGCTGCCTA CCAAGAAGCC CATTCCTGAT TGTGAGATCA AGAATCGGCC CAGCCTCCTG 
GTGGAGAAGA TCAATCTGTT TGCCATGTTT GGCACTGGCA TTGCCATGAG CACCTGGGTC 
TGGACCAAGG CCACCCTGCT CATCTGGAGG CGCACCTGGT GCAGGTTGAC TGGGCACAGT 
GATGATGAAC CCAAGAGAAT CAAGAAAAGC AAGATGATTG CCAAGGCCTT CTCTAAGCGG 
CGTGAACTGC TGCAGAACCC GGGCCAGGAG CTCTCCTTCA GCATGCACAC TGTCTCCCAT 
GATGGACCTG TTGCCGGTTT GGCTTTTGAA CTCAATGAAC CCTCAGCTGA TGTCTCCTCT 
GCCTGGGCCC AGCACGTCAC CAAGATGGTG GCTCGAAGAG GAGCCATATT ACCCCAGGAT 
GTGTCTGTCA CCCCTGTGGC AACTCCAGTG CCACCAGAAG AACAAGCCAA CCTGTGGCTG 
GTTGAGGCAG AGATCTCCCC AGAGTTAGAG AAGCGTTTAG GCCGGAAGAA GAAGCGGAGG 
AAGAGGAAGA AGGAGGTGTG CCCCTTGGGG CCAGCCCCTG AACTTCACCA CTCTGCCCCT 
GTTCCTGCCA CCAGTGCAGT TCCTCGGCTG CCTCAGCTGC CTCGGCAGAA GTGCCTAGTA 
GCTGCAAATG CCTGGGGAAC AGGAGAGCCC TGCCGACAGG GAGCCTGGAC TGTAGTCTCC 
AACCCCTTCT GCCCAGAGCC TAGTCCCCAT CAAGATCCAT TTCTCCCTGG TGCCTCAGCC 
CCCAGGGTCT GGGCTCAGGG CCGCCTCCAG GGGCTGGGAT CCATTCATTC CCGCACTAAC 
CTAATGGAGG CTGAGCTCTT GGATGCAGAC TCGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036939.1
CDS	1..2382
Translation	MAAGRPVRGPELAPRRLLQLLLLVLLGGRGRGAALSGNVTGPGPRSAGGSARRNAPVTSPPPPLLSHCGRAAHCEPLRYNVCLGSALPYGATTTLLAGDSDSQEEAHSKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAIVERERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCGIQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNACFFVGSIGWLAQFMDGARREIVCRADGTMRFGEPTSSETLSCVIIFVIVYYALMAGVVWFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSGICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKINETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKKPIPDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGHSDDEPKRIKKSKMIAKAFSKRRELLQNPGQELSFSMHTVSHDGPVAGLAFELNEPSADVSSAWAQHVTKMVARRGAILPQDVSVTPVATPVPPEEQANLWLVEAEISPELEKRLGRKKKRRKRKKEVCPLGPAPELHHSAPVPATSAVPRLPQLPRQKCLVAANAWGTGEPCRQGAWTVVSNPFCPEPSPHQDPFLPGASAPRVWAQGRLQGLGSIHSRTNLMEAELLDADSDF

Target ORF information:

RefSeq Version	NM_012807.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus smoothened, frizzled class receptor (Smo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012807.1

ORF Insert Sequence:

ATGGCTGCTG GCCGCCCCGT GCGTGGGCCC GAGCTGGCGC CCCGGAGGCT GCTGCAGTTG 
CTGCTGCTGG TACTGCTTGG GGGCCGGGGC CGGGGGGCGG CCTTGAGCGG GAACGTGACC 
GGGCCTGGGC CTCGCAGTGC CGGCGGGAGC GCGAGGAGGA ACGCGCCGGT GACCAGCCCT 
CCGCCGCCGC TGCTGAGCCA CTGCGGCCGG GCCGCCCACT GCGAGCCTTT GCGCTACAAC 
GTGTGCCTGG GCTCCGCGCT GCCCTACGGA GCCACCACCA CGCTGCTGGC TGGGGACTCG 
GACTCGCAGG AGGAAGCGCA CAGCAAGCTC GTGCTCTGGT CCGGCCTCCG GAATGCTCCC 
CGATGCTGGG CAGTGATCCA GCCCCTGCTG TGTGCTGTCT ACATGCCCAA GTGTGAAAAT 
GACCGAGTGG AGTTGCCCAG CCGTACCCTC TGCCAGGCCA CCCGAGGCCC CTGTGCCATT 
GTGGAGCGGG AACGAGGGTG GCCTGACTTT CTGCGTTGCA CGCCGGACCA CTTCCCTGAA 
GGCTGTCCAA ACGAGGTACA AAACATCAAG TTCAACAGTT CAGGCCAATG TGAAGCACCC 
TTGGTGAGGA CAGACAACCC CAAGAGCTGG TACGAGGACG TGGAGGGCTG TGGGATCCAG 
TGCCAGAACC CGCTGTTCAC CGAGGCTGAG CACCAGGACA TGCACAGTTA CATCGCAGCC 
TTCGGGGCGG TCACCGGCCT CTGTACACTC TTCACCCTGG CCACCTTTGT GGCTGACTGG 
CGGAACTCCA ATCGCTACCC TGCGGTTATT CTCTTCTATG TCAATGCGTG TTTCTTTGTG 
GGCAGCATTG GCTGGCTGGC CCAGTTCATG GATGGTGCCC GCCGGGAGAT TGTTTGCCGA 
GCAGATGGCA CCATGAGATT TGGGGAGCCC ACCTCCAGCG AGACCCTATC CTGTGTCATC 
ATCTTTGTCA TCGTGTACTA TGCCTTGATG GCTGGAGTAG TGTGGTTCGT GGTCCTCACC 
TATGCCTGGC ACACCTCCTT CAAAGCCCTG GGCACCACTT ACCAGCCTCT CTCGGGCAAG 
ACATCCTATT TCCACCTGCT CACGTGGTCA CTCCCCTTCG TCCTCACTGT GGCAATCCTT 
GCTGTGGCTC AGGTAGATGG GGACTCCGTG AGTGGCATCT GCTTTGTAGG CTACAAGAAC 
TATCGGTACC GTGCTGGCTT TGTACTTGCC CCAATTGGCC TGGTGCTTAT TGTGGGAGGC 
TACTTCCTCA TCCGAGGGGT CATGACTCTG TTCTCCATCA AGAGCAACCA CCCTGGGCTT 
CTGAGTGAGA AGGCAGCCAG CAAGATCAAT GAGACCATGC TGCGCCTGGG CATTTTTGGC 
TTCCTCGCCT TTGGCTTCGT GCTCATCACC TTCAGCTGCC ACTTCTATGA CTTCTTCAAC 
CAGGCTGAGT GGGAGCGTAG CTTCCGGGAC TATGTGCTAT GCCAAGCCAA TGTGACCATT 
GGGCTGCCTA CCAAGAAGCC CATTCCTGAT TGTGAGATCA AGAATCGGCC CAGCCTCCTG 
GTGGAGAAGA TCAATCTGTT TGCCATGTTT GGCACTGGCA TTGCCATGAG CACCTGGGTC 
TGGACCAAGG CCACCCTGCT CATCTGGAGG CGCACCTGGT GCAGGTTGAC TGGGCACAGT 
GATGATGAAC CCAAGAGAAT CAAGAAAAGC AAGATGATTG CCAAGGCCTT CTCTAAGCGG 
CGTGAACTGC TGCAGAACCC GGGCCAGGAG CTCTCCTTCA GCATGCACAC TGTCTCCCAT 
GATGGACCTG TTGCCGGTTT GGCTTTTGAA CTCAATGAAC CCTCAGCTGA TGTCTCCTCT 
GCCTGGGCCC AGCACGTCAC CAAGATGGTG GCTCGAAGAG GAGCCATATT ACCCCAGGAT 
GTGTCTGTCA CCCCTGTGGC AACTCCAGTG CCACCAGAAG AACAAGCCAA CCTGTGGCTG 
GTTGAGGCAG AGATCTCCCC AGAGTTAGAG AAGCGTTTAG GCCGGAAGAA GAAGCGGAGG 
AAGAGGAAGA AGGAGGTGTG CCCCTTGGGG CCAGCCCCTG AACTTCACCA CTCTGCCCCT 
GTTCCTGCCA CCAGTGCAGT TCCTCGGCTG CCTCAGCTGC CTCGGCAGAA GTGCCTAGTA 
GCTGCAAATG CCTGGGGAAC AGGAGAGCCC TGCCGACAGG GAGCCTGGAC TGTAGTCTCC 
AACCCCTTCT GCCCAGAGCC TAGTCCCCAT CAAGATCCAT TTCTCCCTGG TGCCTCAGCC 
CCCAGGGTCT GGGCTCAGGG CCGCCTCCAG GGGCTGGGAT CCATTCATTC CCGCACTAAC 
CTAATGGAGG CTGAGCTCTT GGATGCAGAC TCGGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tubby family proteins are adapters for ciliary trafficking of integral membrane proteins.
The Journal of cell biology216(3)743-760(2017 Mar)
Badgandi HB,Hwang SH,Shimada IS,Loriot E,Mukhopadhyay S

Intrinsic facilitation of adult peripheral nerve regeneration by the Sonic hedgehog morphogen.
Experimental neurology271493-505(2015 Sep)
Martinez JA,Kobayashi M,Krishnan A,Webber C,Christie K,Guo G,Singh V,Zochodne DW

FGFR3 Deficiency Causes Multiple Chondroma-like Lesions by Upregulating Hedgehog Signaling.
PLoS genetics11(6)e1005214(2015 Jun)
Zhou S,Xie Y,Tang J,Huang J,Huang Q,Xu W,Wang Z,Luo F,Wang Q,Chen H,Du X,Shen Y,Chen D,Chen L

Bifurcating action of Smoothened in Hedgehog signaling is mediated by Dlg5.
Genes & development29(3)262-76(2015 Feb)
Chong YC,Mann RK,Zhao C,Kato M,Beachy PA

Expression of hedgehog signal pathway in articular cartilage is associated with the severity of cartilage damage in rats with adjuvant-induced arthritis.
Journal of inflammation (London, England)1224(2015)
Li R,Cai L,Hu CM,Wu TN,Li J

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Smo cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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