Gpc3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpc3
Entrez Gene ID	25236
Full Name	glypican 3
Synonyms	OCI-5
General protein information	Preferred Names glypican 3 Names glypican-3 defective in Simpson-Golabi-Behmel overgrowth syndrome intestinal protein OCI-5 proteoglycan GPC3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq36
Summary	heparan sulfate cell surface proteogycan, may be involved in development of mesodermal tissues, may modulate Igf2 function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012774.1	NP_036906.1	glypican-3 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gpc3	NP_057906.2
Pan troglodytes (chimpanzee)	GPC3	NP_001129209.1
Canis lupus familiaris (dog)	GPC3	XP_538178.2
Gallus gallus (chicken)	GPC3	XP_001232892.1
	GPC3	XP_001097558.2
Danio rerio (zebrafish)	gpc3	XP_688014.3
Homo sapiens (human)	GPC3	NP_004475.1
Bos taurus (cattle)	GPC3	NP_001030540.1
Xenopus tropicalis (tropical clawed frog)	gpc3	XP_002932665.2
Rattus norvegicus (Norway rat)	Gpc3	NP_036906.1

Gene Ontology
Bibliography

Related articles in PubMed

Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma.
Zaghloul RA, El-Shishtawy MM, El Galil KH, Ebrahim MA, Metwaly AA, Al-Gayyar MM
European journal of pharmacology746353-62(2015 Jan)

Glypican-3 binds to Frizzled and plays a direct role in the stimulation of canonical Wnt signaling.
Capurro M, Martin T, Shi W, Filmus J
Journal of cell science127(Pt 7)1565-75(2014 Apr)

Impact of lactobacilli on orally acquired listeriosis.
Archambaud C, Nahori MA, Soubigou G, B?cavin C, Laval L, Lechat P, Smokvina T, Langella P, Lecuit M, Cossart P
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)

Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma.
Yao M, Yao DF, Bian YZ, Zhang CG, Qiu LW, Wu W, Sai WL, Yang JL, Zhang HJ
Hepatobiliary & pancreatic diseases international : HBPD INT10(3)289-94(2011 Jun)

Dally-like core protein and its mammalian homologues mediate stimulatory and inhibitory effects on Hedgehog signal response.
Williams EH, Pappano WN, Saunders AM, Kim MS, Leahy DJ, Beachy PA
Proceedings of the National Academy of Sciences of the United States of America107(13)5869-74(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of hepatocellular carcinoma.
Title: Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma.

GPC3 may be a negative regulator of liver regeneration and hepatocyte proliferation, and that this regulation may involve CD81.
Title: Investigation of the role of glypican 3 in liver regeneration and hepatocyte proliferation.

Gpc3 is a marker of hepatic progenitor cells and of early liver lesions.
Title: The oncofetal protein glypican-3 is a novel marker of hepatic progenitor/oval cells.

The following Gpc3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpc3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00899	NM_012774.1 Latest version!	Rattus norvegicus glypican 3 (Gpc3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpc3 ( NM_012774.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036906.1 Rattus norvegicus glypican 3 (Gpc3), mRNA.

Price & Availability↑

CloneID	ORa00899
Clone ID Related Accession (Same CDS sequence)	NM_012774.1
Accession Version	NM_012774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glypican-3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M22400.1. On Apr 2, 2005 this sequence version replaced XM_346357.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M22400.1, BC085756.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906348, SAMN01906349 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGGA CCGTGCGCAC CGCGTGCTTG CTGGTGGCGA TGCTGCTCGG CTTGGGCTGC 
CTGGGACAGG CGCAGCCCCC GCCGCCTCCA GACGCCACCT GTCACCAGGT CCGTTCTTTC 
TTCCAGAGAC TGCAGCCCGG ACTCAAATGG GTTCCAGAAA CCCCTGTGCC AGGATCAGAT 
TTGCAAGTAT GTCTCCCCAA GGGCCCAACA TGCTGCTCAA GAAAGATGGA AGAAAAATAC 
CAACTAACAG CGCGCCTGAA CATGGAACAA CTGCTCCAGT CTGCGAGTAT GGAACTCAAG 
TTCTTAATTA TTCAGAATGC TGCGGTTTTC CAAGAGGCCT TTGAAATTGT TGTTCGCCAT 
GCCAAGAACT ACACCAATGC CATGTTCAAG AATAACTACC CCAGCCTGAC TCCACAAGCT 
TTTGAGTTTG TCGGTGAATT TTTCACAGAT GTGTCTCTCT ACATCTTGGG TTCTGATATC 
AATGTGGATG ATATGGTCAA TGAATTGTTC GACAGCCTCT TTCCAGTCAT CTATACCCAG 
ATGATGAACC CAGGCCTCCC CGAGTCAGTA TTAGACATCA ACGAGTGCCT CCGAGGAGCA 
AGACGCGACC TGAAAGTATT TGGCAGTTTC CCCAAGCTTA TTATGACCCA GGTTTCCAAG 
TCACTGCAAG TTACTAGAAT CTTCCTTCAG GCCCTGAACC TCGGAATTGA AGTAATCAAC 
ACCACCGACC ACCTCAAGTT TAGTAAGGAC TGTGGCCGTA TGCTCACCCG AATGTGGTAC 
TGCTCTTACT GCCAGGGACT GATGATGGTC AAACCTTGTG GTGGTTATTG CAATGTGGTC 
ATGCAAGGCT GTATGGCAGG TGTGGTAGAG ATCGACAAGT ACTGGAGAGA ATACATTCTG 
TCTCTTGAAG AGCTCGTGAA CGGCATGTAC AGAATCTACG ACATGGAGAA TGTGCTGCTC 
GGACTCTTTT CAACCATCCA TGATTCCATC CAGTATGTGC AGAAGAACGG AGGCAAGCTG 
ACCACCACTA TTGGCAAGTT GTGCGCCCAC TCCCAGCAAC GCCAATATAG ATCTGCTTAT 
TATCCTGAAG ATCTGTTTAT TGACAAGAAG GTATTAAAAG TCGCCCGTGT CGAACATGAA 
GAAACCTTAT CCAGCCGAAG AAGGGAACTG ATTCAGAAGT TGAAGTCTTT CATCAGCTTC 
TATAGTGCTT TGCCAGGCTA CATCTGCAGC CATAGCCCCG TGGCCGAAAA CGACACCCTG 
TGCTGGAACG GACAAGAGCT TGTGGAGAGA TACAGCCAGA AGGCGGCAAG GAATGGGATG 
AAGAATCAGT TTAACCTCCA TGAGCTGAAA ATGAAGGGCC CTGAGCCAGT GGTTAGCCAG 
ATCATTGACA AACTGAAGCA CATTAACCAG CTCCTGAGAA CCATGTCTGT GCCCAAGGGT 
AAAGTTGTGG ATAAAAGCCT GGATGAGGAA GGACTTGAGA GTGGAGACTG TGGTGATGAT 
GAGGATGAGT GCATCGGGAG CTCTGGTGAC GGCATGATGA AAGTGAAGAA CCAACTGCGC 
TTCCTTGCAG AACTGGCATA TGATCTGGAT GTGGACGATG CTCCAGGGAA CAAGCAACAT 
GGAAATCAGA AGGACAACGA GATCACCACC TCTCACAGCG TGGGGAACAT GCCATCCCCA 
CTGAAAATCC TCATCAGTGT GGCCATCTAC GTGGCGTGCT TTTTTTCCTG GTGCACTGAC 
TTGCCATGCC CATGCCTGTG CTGCCCTGCA GCACCTTGTG GCCCTCCTAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036906.1
CDS	115..1908
Translation	MAGTVRTACLLVAMLLGLGCLGQAQPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQMMNPGLPESVLDINECLRGARRDLKVFGSFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNGGKLTTTIGKLCAHSQQRQYRSAYYPEDLFIDKKVLKVARVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSVPKGKVVDKSLDEEGLESGDCGDDEDECIGSSGDGMMKVKNQLRFLAELAYDLDVDDAPGNKQHGNQKDNEITTSHSVGNMPSPLKILISVAIYVACFFSWCTDLPCPCLCCPAAPCGPPT

Target ORF information:

RefSeq Version	NM_012774.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glypican 3 (Gpc3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012774.1

ORF Insert Sequence:

ATGGCCGGGA CCGTGCGCAC CGCGTGCTTG CTGGTGGCGA TGCTGCTCGG CTTGGGCTGC 
CTGGGACAGG CGCAGCCCCC GCCGCCTCCA GACGCCACCT GTCACCAGGT CCGTTCTTTC 
TTCCAGAGAC TGCAGCCCGG ACTCAAATGG GTTCCAGAAA CCCCTGTGCC AGGATCAGAT 
TTGCAAGTAT GTCTCCCCAA GGGCCCAACA TGCTGCTCAA GAAAGATGGA AGAAAAATAC 
CAACTAACAG CGCGCCTGAA CATGGAACAA CTGCTCCAGT CTGCGAGTAT GGAACTCAAG 
TTCTTAATTA TTCAGAATGC TGCGGTTTTC CAAGAGGCCT TTGAAATTGT TGTTCGCCAT 
GCCAAGAACT ACACCAATGC CATGTTCAAG AATAACTACC CCAGCCTGAC TCCACAAGCT 
TTTGAGTTTG TCGGTGAATT TTTCACAGAT GTGTCTCTCT ACATCTTGGG TTCTGATATC 
AATGTGGATG ATATGGTCAA TGAATTGTTC GACAGCCTCT TTCCAGTCAT CTATACCCAG 
ATGATGAACC CAGGCCTCCC CGAGTCAGTA TTAGACATCA ACGAGTGCCT CCGAGGAGCA 
AGACGCGACC TGAAAGTATT TGGCAGTTTC CCCAAGCTTA TTATGACCCA GGTTTCCAAG 
TCACTGCAAG TTACTAGAAT CTTCCTTCAG GCCCTGAACC TCGGAATTGA AGTAATCAAC 
ACCACCGACC ACCTCAAGTT TAGTAAGGAC TGTGGCCGTA TGCTCACCCG AATGTGGTAC 
TGCTCTTACT GCCAGGGACT GATGATGGTC AAACCTTGTG GTGGTTATTG CAATGTGGTC 
ATGCAAGGCT GTATGGCAGG TGTGGTAGAG ATCGACAAGT ACTGGAGAGA ATACATTCTG 
TCTCTTGAAG AGCTCGTGAA CGGCATGTAC AGAATCTACG ACATGGAGAA TGTGCTGCTC 
GGACTCTTTT CAACCATCCA TGATTCCATC CAGTATGTGC AGAAGAACGG AGGCAAGCTG 
ACCACCACTA TTGGCAAGTT GTGCGCCCAC TCCCAGCAAC GCCAATATAG ATCTGCTTAT 
TATCCTGAAG ATCTGTTTAT TGACAAGAAG GTATTAAAAG TCGCCCGTGT CGAACATGAA 
GAAACCTTAT CCAGCCGAAG AAGGGAACTG ATTCAGAAGT TGAAGTCTTT CATCAGCTTC 
TATAGTGCTT TGCCAGGCTA CATCTGCAGC CATAGCCCCG TGGCCGAAAA CGACACCCTG 
TGCTGGAACG GACAAGAGCT TGTGGAGAGA TACAGCCAGA AGGCGGCAAG GAATGGGATG 
AAGAATCAGT TTAACCTCCA TGAGCTGAAA ATGAAGGGCC CTGAGCCAGT GGTTAGCCAG 
ATCATTGACA AACTGAAGCA CATTAACCAG CTCCTGAGAA CCATGTCTGT GCCCAAGGGT 
AAAGTTGTGG ATAAAAGCCT GGATGAGGAA GGACTTGAGA GTGGAGACTG TGGTGATGAT 
GAGGATGAGT GCATCGGGAG CTCTGGTGAC GGCATGATGA AAGTGAAGAA CCAACTGCGC 
TTCCTTGCAG AACTGGCATA TGATCTGGAT GTGGACGATG CTCCAGGGAA CAAGCAACAT 
GGAAATCAGA AGGACAACGA GATCACCACC TCTCACAGCG TGGGGAACAT GCCATCCCCA 
CTGAAAATCC TCATCAGTGT GGCCATCTAC GTGGCGTGCT TTTTTTCCTG GTGCACTGAC 
TTGCCATGCC CATGCCTGTG CTGCCCTGCA GCACCTTGTG GCCCTCCTAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinoma.
European journal of pharmacology746353-62(2015 Jan)
Zaghloul RA,El-Shishtawy MM,El Galil KH,Ebrahim MA,Metwaly AA,Al-Gayyar MM

Glypican-3 binds to Frizzled and plays a direct role in the stimulation of canonical Wnt signaling.
Journal of cell science127(Pt 7)1565-75(2014 Apr)
Capurro M,Martin T,Shi W,Filmus J

Impact of lactobacilli on orally acquired listeriosis.
Proceedings of the National Academy of Sciences of the United States of America109(41)16684-9(2012 Oct)
Archambaud C,Nahori MA,Soubigou G,B?cavin C,Laval L,Lechat P,Smokvina T,Langella P,Lecuit M,Cossart P

Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma.
Hepatobiliary & pancreatic diseases international : HBPD INT10(3)289-94(2011 Jun)
Yao M,Yao DF,Bian YZ,Zhang CG,Qiu LW,Wu W,Sai WL,Yang JL,Zhang HJ

Dally-like core protein and its mammalian homologues mediate stimulatory and inhibitory effects on Hedgehog signal response.
Proceedings of the National Academy of Sciences of the United States of America107(13)5869-74(2010 Mar)
Williams EH,Pappano WN,Saunders AM,Kim MS,Leahy DJ,Beachy PA

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Gpc3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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