Akt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akt2
Entrez Gene ID	25233
Full Name	AKT serine/threonine kinase 2
General protein information	Preferred Names AKT serine/threonine kinase 2 Names RAC-beta serine/threonine-protein kinase AKT2 kinase PKB beta RAC protein kinase beta RAC-PK beta RAC-PK-beta murine thymoma viral (v-akt) oncogene homolog 2 protein kinase Akt-2 protein kinase B beta thymoma viral proto-oncogene 2 v-akt murine thymoma viral oncogene homolog 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	may be involved in phosphatidylinositol 3-kinase (PI3-K) mediated signaling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017093.1	NP_058789.1	RAC-beta serine/threonine-protein kinase
XM_008759108.2	XP_008757330.1	RAC-beta serine/threonine-protein kinase isoform X1
XM_017588811.1	XP_017444300.1	RAC-beta serine/threonine-protein kinase isoform X1
XM_008759110.2	XP_008757332.1	RAC-beta serine/threonine-protein kinase isoform X2
XM_008759111.2	XP_008757333.1	RAC-beta serine/threonine-protein kinase isoform X2
XM_008759109.2	XP_008757331.1	RAC-beta serine/threonine-protein kinase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	AKT2	NP_001617.1
Mus musculus (house mouse)	Akt2	NP_001103678.1
Bos taurus (cattle)	AKT2	NP_001193075.1
Danio rerio (zebrafish)	akt2	NP_937789.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002161	XP_308030.5
Rattus norvegicus (Norway rat)	Akt2	NP_058789.1
Pan troglodytes (chimpanzee)	AKT2	XP_001143709.2
	AKT2	XP_001093461.1
Canis lupus familiaris (dog)	AKT2	NP_001012340.1
Xenopus tropicalis (tropical clawed frog)	akt2	NP_001116936.1
Drosophila melanogaster (fruit fly)	Akt1	NP_732114.1

Gene Ontology
Bibliography

Related articles in PubMed

Akt2-Dependent Beneficial Effect of Galanin on Insulin-Induced Glucose Uptake in Adipocytes of Diabetic Rats.
Zhang Z, Fang P, Guo L, He B, Shi M, Zhu Y, Bo P
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology41(5)1777-1787(2017)

Activation of PI3K?/Akt pathway increases cardiomyocyte HMGB1 expression in diabetic environment.
Song J, Liu Q, Tang H, Tao A, Wang H, Kao R, Rui T
Oncotarget7(49)80803-80810(2016 Dec)

Inhibition of Akt2 phosphorylation abolishes the calorie restriction-induced improvement in insulin-stimulated glucose uptake by rat soleus muscle.
Sharma N, Arias EB, Cartee GD
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme41(11)1208-1211(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results of this study suggest that phosphorylation of Akt2 mediates the beneficial effects of galanin on insulin-induced glucose uptake in the adipocytes of diabetic rats.
Title: Akt2-Dependent Beneficial Effect of Galanin on Insulin-Induced Glucose Uptake in Adipocytes of Diabetic Rats.

Data suggest that caloric restriction in aging rats up-regulates insulin-stimulated glucose uptake in the predominantly slow-twitch soleus muscle; this up-regulation appears to rely on enhanced Akt2 phosphorylation.
Title: Inhibition of Akt2 phosphorylation abolishes the calorie restriction-induced improvement in insulin-stimulated glucose uptake by rat soleus muscle.

Evidence links greater Akt2 activation to the caloric restriction-induced elevation of insulin-stimulated glucose uptake by muscle from old animals.
Title: Calorie restriction leads to greater Akt2 activity and glucose uptake by insulin-stimulated skeletal muscle from old rats.

Role of the guanine nucleotide exchange factor in Akt2-mediated plasma membrane translocation of GLUT4 in insulin-stimulated skeletal muscle.
Title: Role of the guanine nucleotide exchange factor in Akt2-mediated plasma membrane translocation of GLUT4 in insulin-stimulated skeletal muscle.

PP2A inhibitors increased hepatic Akt phosphorylation and inhibited FoxO1in vitro and in vivo, and suppressed gluconeogenesis in hepatocytes
Title: PP2A inhibition results in hepatic insulin resistance despite Akt2 activation.

The following Akt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12709	NM_017093.1 Latest version!	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22471	XM_008759108.2 Latest version!	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22471	XM_017588811.1 Latest version!	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22472	XM_008759109.2 Latest version!	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22472	XM_008759111.2 Latest version!	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa22472	XM_008759110.2 Latest version!	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akt2 ( NM_017093.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058789.1 Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), mRNA.

Price & Availability↑

CloneID	ORa12709
Clone ID Related Accession (Same CDS sequence)	NM_017093.1
Accession Version	NM_017093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	RAC-beta serine/threonine-protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D30041.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D30041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGACCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGTGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGACGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTC CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCCAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCC 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058789.1
CDS	282..1727
Translation	MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWIRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGDLFFHLSRERVFTEDRARFYGAEIVSALEYLHSTDVVYRDIKLENLMLDKDGHIKITDFGLSKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGSLELDQRTHFPQFSYSASIRE

Target ORF information:

RefSeq Version	NM_017093.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017093.1

ORF Insert Sequence:

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGACCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGTGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGACGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTC CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCCAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCC 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt2 ( XM_008759108.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757330.1 Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22471
Clone ID Related Accession (Same CDS sequence)	XM_008759108.2 , XM_017588811.1
Accession Version	XM_008759108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RAC-beta serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTACC CCAGCCTGCG TCAGCAGGTG CTACCTCCAA GATTCCCTCG GGAGATCCTC 
TCATCTACCT TCCTCCCAAT GTGGTACCCT TGTGAATACA TCAAGACCTG GAGGCCCCGG 
TACTTCCTTC TGAAGAGTGA TGGATCTTTT ATTGGGTATA AAGAGAGACC TGAGGCCCCC 
GACCAGACCC TACCCCCCCT TAACAATTTC TCTGTAGCAG AATGCCAGCT GATGAAGACT 
GAGAGGCCAC GACCCAACAC CTTTGTCATA CGCTGCCTGC AGTGGACCAC AGTCATCGAG 
AGGACCTTCC ATGTCGACTC TCCAGATGAG AGGGAAGAGT GGATACGGGC TATCCAGATG 
GTAGCCAACA GTCTGAAGCA GCGGGGCCCC GGGGAGGATG CCATGGATTA CAAGTGTGGC 
TCCCCCAGTG ACTCTTCCAC ATCTGAGATG ATGGAGGTAG CGGTCAGCAA GGCACGGGCC 
AAAGTGACCA TGAACGACTT CGATTATCTC AAACTCCTCG GCAAGGGCAC CTTCGGCAAA 
GTCATTCTAG TTCGAGAGAA GGCCACTGGC CGCTATTATG CCATGAAGAT CCTGCGGAAG 
GAGGTCATCA TTGCAAAGGA TGAAGTCGCC CACACAGTCA CAGAGAGCCG AGTCCTACAG 
AATACCAGGC ACCCCTTCCT TACAGCCCTG AAGTATGCCT TCCAGACCCA TGACCGCCTG 
TGCTTTGTGA TGGAGTATGC CAACGGGGGC GAGCTGTTTT TCCACCTCTC TCGGGAGCGA 
GTCTTCACGG AGGATCGGGC ACGCTTTTAT GGAGCAGAGA TTGTGTCAGC TCTGGAGTAC 
TTGCACTCGA GGGATGTGGT GTACCGCGAC ATCAAGCTGG AAAACCTTAT GCTGGACAAA 
GATGGCCACA TCAAGATCAC TGACTTTGGC TTGTGCAAAG AGGGCATCAG TGATGGGGCC 
ACCATGAAAA CCTTCTGTGG TACCCCGGAG TACTTGGCGC CTGAGGTGCT AGAGGACAAT 
GACTACGGGC GTGCAGTGGA CTGGTGGGGA CTGGGCGTGG TCATGTACGA GATGATGTGC 
GGCCGCCTGC CCTTCTACAA CCAGGACCAC GAGCGCCTCT TCGAGCTCAT CCTCATGGAG 
GAGATCCGGT TCCCACGCAC GCTTGGGCCA GAGGCCAAGT CCCTGCTGGC TGGACTGCTC 
AAGAAGGACC CTAAGCAGAG GCTCGGTGGG GGTCCCAGTG ATGCAAAGGA GGTCATGGAG 
CATCGGTTCT TCCTCAGCAT CAACTGGCAG GATGTGGTAC AGAAGAAGCT CCTGCCACCC 
TTCAAACCTC AGGTCACTTC AGAAGTGGAC ACAAGGTACT TTGATGATGA ATTCACCGCC 
CAGTCCATCA CAATCACACC CCCAGACCGA TATGACAGCC TGGGCTCGCT GGAACTGGAC 
CAGCGCACAC ACTTCCCCCA GTTCTCCTAC TCAGCCAGCA TCCGAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757330.1
CDS	2635..4125
Translation	MQYPSLRQQVLPPRFPREILSSTFLPMWYPCEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWIRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGSLELDQRTHFPQFSYSASIRE

Target ORF information:

RefSeq Version	XM_008759108.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759108.2

ORF Insert Sequence:

ATGCAGTACC CCAGCCTGCG TCAGCAGGTG CTACCTCCAA GATTCCCTCG GGAGATCCTC 
TCATCTACCT TCCTCCCAAT GTGGTACCCT TGTGAATACA TCAAGACCTG GAGGCCCCGG 
TACTTCCTTC TGAAGAGTGA TGGATCTTTT ATTGGGTATA AAGAGAGACC TGAGGCCCCC 
GACCAGACCC TACCCCCCCT TAACAATTTC TCTGTAGCAG AATGCCAGCT GATGAAGACT 
GAGAGGCCAC GACCCAACAC CTTTGTCATA CGCTGCCTGC AGTGGACCAC AGTCATCGAG 
AGGACCTTCC ATGTCGACTC TCCAGATGAG AGGGAAGAGT GGATACGGGC TATCCAGATG 
GTAGCCAACA GTCTGAAGCA GCGGGGCCCC GGGGAGGATG CCATGGATTA CAAGTGTGGC 
TCCCCCAGTG ACTCTTCCAC ATCTGAGATG ATGGAGGTAG CGGTCAGCAA GGCACGGGCC 
AAAGTGACCA TGAACGACTT CGATTATCTC AAACTCCTCG GCAAGGGCAC CTTCGGCAAA 
GTCATTCTAG TTCGAGAGAA GGCCACTGGC CGCTATTATG CCATGAAGAT CCTGCGGAAG 
GAGGTCATCA TTGCAAAGGA TGAAGTCGCC CACACAGTCA CAGAGAGCCG AGTCCTACAG 
AATACCAGGC ACCCCTTCCT TACAGCCCTG AAGTATGCCT TCCAGACCCA TGACCGCCTG 
TGCTTTGTGA TGGAGTATGC CAACGGGGGC GAGCTGTTTT TCCACCTCTC TCGGGAGCGA 
GTCTTCACGG AGGATCGGGC ACGCTTTTAT GGAGCAGAGA TTGTGTCAGC TCTGGAGTAC 
TTGCACTCGA GGGATGTGGT GTACCGCGAC ATCAAGCTGG AAAACCTTAT GCTGGACAAA 
GATGGCCACA TCAAGATCAC TGACTTTGGC TTGTGCAAAG AGGGCATCAG TGATGGGGCC 
ACCATGAAAA CCTTCTGTGG TACCCCGGAG TACTTGGCGC CTGAGGTGCT AGAGGACAAT 
GACTACGGGC GTGCAGTGGA CTGGTGGGGA CTGGGCGTGG TCATGTACGA GATGATGTGC 
GGCCGCCTGC CCTTCTACAA CCAGGACCAC GAGCGCCTCT TCGAGCTCAT CCTCATGGAG 
GAGATCCGGT TCCCACGCAC GCTTGGGCCA GAGGCCAAGT CCCTGCTGGC TGGACTGCTC 
AAGAAGGACC CTAAGCAGAG GCTCGGTGGG GGTCCCAGTG ATGCAAAGGA GGTCATGGAG 
CATCGGTTCT TCCTCAGCAT CAACTGGCAG GATGTGGTAC AGAAGAAGCT CCTGCCACCC 
TTCAAACCTC AGGTCACTTC AGAAGTGGAC ACAAGGTACT TTGATGATGA ATTCACCGCC 
CAGTCCATCA CAATCACACC CCCAGACCGA TATGACAGCC TGGGCTCGCT GGAACTGGAC 
CAGCGCACAC ACTTCCCCCA GTTCTCCTAC TCAGCCAGCA TCCGAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt2 ( XM_017588811.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444300.1 Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22471
Clone ID Related Accession (Same CDS sequence)	XM_008759108.2 , XM_017588811.1
Accession Version	XM_017588811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RAC-beta serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTACC CCAGCCTGCG TCAGCAGGTG CTACCTCCAA GATTCCCTCG GGAGATCCTC 
TCATCTACCT TCCTCCCAAT GTGGTACCCT TGTGAATACA TCAAGACCTG GAGGCCCCGG 
TACTTCCTTC TGAAGAGTGA TGGATCTTTT ATTGGGTATA AAGAGAGACC TGAGGCCCCC 
GACCAGACCC TACCCCCCCT TAACAATTTC TCTGTAGCAG AATGCCAGCT GATGAAGACT 
GAGAGGCCAC GACCCAACAC CTTTGTCATA CGCTGCCTGC AGTGGACCAC AGTCATCGAG 
AGGACCTTCC ATGTCGACTC TCCAGATGAG AGGGAAGAGT GGATACGGGC TATCCAGATG 
GTAGCCAACA GTCTGAAGCA GCGGGGCCCC GGGGAGGATG CCATGGATTA CAAGTGTGGC 
TCCCCCAGTG ACTCTTCCAC ATCTGAGATG ATGGAGGTAG CGGTCAGCAA GGCACGGGCC 
AAAGTGACCA TGAACGACTT CGATTATCTC AAACTCCTCG GCAAGGGCAC CTTCGGCAAA 
GTCATTCTAG TTCGAGAGAA GGCCACTGGC CGCTATTATG CCATGAAGAT CCTGCGGAAG 
GAGGTCATCA TTGCAAAGGA TGAAGTCGCC CACACAGTCA CAGAGAGCCG AGTCCTACAG 
AATACCAGGC ACCCCTTCCT TACAGCCCTG AAGTATGCCT TCCAGACCCA TGACCGCCTG 
TGCTTTGTGA TGGAGTATGC CAACGGGGGC GAGCTGTTTT TCCACCTCTC TCGGGAGCGA 
GTCTTCACGG AGGATCGGGC ACGCTTTTAT GGAGCAGAGA TTGTGTCAGC TCTGGAGTAC 
TTGCACTCGA GGGATGTGGT GTACCGCGAC ATCAAGCTGG AAAACCTTAT GCTGGACAAA 
GATGGCCACA TCAAGATCAC TGACTTTGGC TTGTGCAAAG AGGGCATCAG TGATGGGGCC 
ACCATGAAAA CCTTCTGTGG TACCCCGGAG TACTTGGCGC CTGAGGTGCT AGAGGACAAT 
GACTACGGGC GTGCAGTGGA CTGGTGGGGA CTGGGCGTGG TCATGTACGA GATGATGTGC 
GGCCGCCTGC CCTTCTACAA CCAGGACCAC GAGCGCCTCT TCGAGCTCAT CCTCATGGAG 
GAGATCCGGT TCCCACGCAC GCTTGGGCCA GAGGCCAAGT CCCTGCTGGC TGGACTGCTC 
AAGAAGGACC CTAAGCAGAG GCTCGGTGGG GGTCCCAGTG ATGCAAAGGA GGTCATGGAG 
CATCGGTTCT TCCTCAGCAT CAACTGGCAG GATGTGGTAC AGAAGAAGCT CCTGCCACCC 
TTCAAACCTC AGGTCACTTC AGAAGTGGAC ACAAGGTACT TTGATGATGA ATTCACCGCC 
CAGTCCATCA CAATCACACC CCCAGACCGA TATGACAGCC TGGGCTCGCT GGAACTGGAC 
CAGCGCACAC ACTTCCCCCA GTTCTCCTAC TCAGCCAGCA TCCGAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444300.1
CDS	478..1968
Translation	MQYPSLRQQVLPPRFPREILSSTFLPMWYPCEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWIRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGSLELDQRTHFPQFSYSASIRE

Target ORF information:

RefSeq Version	XM_017588811.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017588811.1

ORF Insert Sequence:

ATGCAGTACC CCAGCCTGCG TCAGCAGGTG CTACCTCCAA GATTCCCTCG GGAGATCCTC 
TCATCTACCT TCCTCCCAAT GTGGTACCCT TGTGAATACA TCAAGACCTG GAGGCCCCGG 
TACTTCCTTC TGAAGAGTGA TGGATCTTTT ATTGGGTATA AAGAGAGACC TGAGGCCCCC 
GACCAGACCC TACCCCCCCT TAACAATTTC TCTGTAGCAG AATGCCAGCT GATGAAGACT 
GAGAGGCCAC GACCCAACAC CTTTGTCATA CGCTGCCTGC AGTGGACCAC AGTCATCGAG 
AGGACCTTCC ATGTCGACTC TCCAGATGAG AGGGAAGAGT GGATACGGGC TATCCAGATG 
GTAGCCAACA GTCTGAAGCA GCGGGGCCCC GGGGAGGATG CCATGGATTA CAAGTGTGGC 
TCCCCCAGTG ACTCTTCCAC ATCTGAGATG ATGGAGGTAG CGGTCAGCAA GGCACGGGCC 
AAAGTGACCA TGAACGACTT CGATTATCTC AAACTCCTCG GCAAGGGCAC CTTCGGCAAA 
GTCATTCTAG TTCGAGAGAA GGCCACTGGC CGCTATTATG CCATGAAGAT CCTGCGGAAG 
GAGGTCATCA TTGCAAAGGA TGAAGTCGCC CACACAGTCA CAGAGAGCCG AGTCCTACAG 
AATACCAGGC ACCCCTTCCT TACAGCCCTG AAGTATGCCT TCCAGACCCA TGACCGCCTG 
TGCTTTGTGA TGGAGTATGC CAACGGGGGC GAGCTGTTTT TCCACCTCTC TCGGGAGCGA 
GTCTTCACGG AGGATCGGGC ACGCTTTTAT GGAGCAGAGA TTGTGTCAGC TCTGGAGTAC 
TTGCACTCGA GGGATGTGGT GTACCGCGAC ATCAAGCTGG AAAACCTTAT GCTGGACAAA 
GATGGCCACA TCAAGATCAC TGACTTTGGC TTGTGCAAAG AGGGCATCAG TGATGGGGCC 
ACCATGAAAA CCTTCTGTGG TACCCCGGAG TACTTGGCGC CTGAGGTGCT AGAGGACAAT 
GACTACGGGC GTGCAGTGGA CTGGTGGGGA CTGGGCGTGG TCATGTACGA GATGATGTGC 
GGCCGCCTGC CCTTCTACAA CCAGGACCAC GAGCGCCTCT TCGAGCTCAT CCTCATGGAG 
GAGATCCGGT TCCCACGCAC GCTTGGGCCA GAGGCCAAGT CCCTGCTGGC TGGACTGCTC 
AAGAAGGACC CTAAGCAGAG GCTCGGTGGG GGTCCCAGTG ATGCAAAGGA GGTCATGGAG 
CATCGGTTCT TCCTCAGCAT CAACTGGCAG GATGTGGTAC AGAAGAAGCT CCTGCCACCC 
TTCAAACCTC AGGTCACTTC AGAAGTGGAC ACAAGGTACT TTGATGATGA ATTCACCGCC 
CAGTCCATCA CAATCACACC CCCAGACCGA TATGACAGCC TGGGCTCGCT GGAACTGGAC 
CAGCGCACAC ACTTCCCCCA GTTCTCCTAC TCAGCCAGCA TCCGAGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt2 ( XM_008759110.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757332.1 Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa22472
Clone ID Related Accession (Same CDS sequence)	XM_008759110.2 , XM_008759111.2 , XM_008759109.2
Accession Version	XM_008759110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RAC-beta serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGAGCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGAGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGAGGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTG CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCTAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCA 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757332.1
CDS	2547..3992
Translation	MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWIRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGSLELDQRTHFPQFSYSASIRE

Target ORF information:

RefSeq Version	XM_008759110.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759110.2

ORF Insert Sequence:

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGAGCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGAGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGAGGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTG CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCTAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCA 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt2 ( XM_008759111.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757333.1 Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa22472
Clone ID Related Accession (Same CDS sequence)	XM_008759110.2 , XM_008759111.2 , XM_008759109.2
Accession Version	XM_008759111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RAC-beta serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGAGCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGAGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGAGGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTG CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCTAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCA 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757333.1
CDS	390..1835
Translation	MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWIRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGSLELDQRTHFPQFSYSASIRE

Target ORF information:

RefSeq Version	XM_008759111.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759111.2

ORF Insert Sequence:

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGAGCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGAGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGAGGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTG CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCTAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCA 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Akt2 ( XM_008759109.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757331.1 Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa22472
Clone ID Related Accession (Same CDS sequence)	XM_008759110.2 , XM_008759111.2 , XM_008759109.2
Accession Version	XM_008759109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1446bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RAC-beta serine/threonine-protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008759109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGAGCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGAGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGAGGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTG CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCTAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCA 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757331.1
CDS	270..1715
Translation	MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWIRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGSLELDQRTHFPQFSYSASIRE

Target ORF information:

RefSeq Version	XM_008759109.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus AKT serine/threonine kinase 2 (Akt2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759109.2

ORF Insert Sequence:

ATGAATGAGG TATCTGTCAT CAAAGAAGGC TGGCTCCACA AACGTGGTGA ATACATCAAG 
ACCTGGAGGC CCCGGTACTT CCTTCTGAAG AGTGATGGAT CTTTTATTGG GTATAAAGAG 
AGACCTGAGG CCCCCGACCA GACCCTACCC CCCCTTAACA ATTTCTCTGT AGCAGAATGC 
CAGCTGATGA AGACTGAGAG GCCACGACCC AACACCTTTG TCATACGCTG CCTGCAGTGG 
ACCACAGTCA TCGAGAGGAC CTTCCATGTC GACTCTCCAG ATGAGAGGGA AGAGTGGATA 
CGGGCTATCC AGATGGTAGC CAACAGTCTG AAGCAGCGGG GCCCCGGGGA GGATGCCATG 
GATTACAAGT GTGGCTCCCC CAGTGACTCT TCCACATCTG AGATGATGGA GGTAGCGGTC 
AGCAAGGCAC GGGCCAAAGT GACCATGAAC GACTTCGATT ATCTCAAACT CCTCGGCAAG 
GGCACCTTCG GCAAAGTCAT TCTAGTTCGA GAGAAGGCCA CTGGCCGCTA TTATGCCATG 
AAGATCCTGC GGAAGGAGGT CATCATTGCA AAGGATGAAG TCGCCCACAC AGTCACAGAG 
AGCCGAGTCC TACAGAATAC CAGGCACCCC TTCCTTACAG CCCTGAAGTA TGCCTTCCAG 
ACCCATGACC GCCTGTGCTT TGTGATGGAG TATGCCAACG GGGGCGAGCT GTTTTTCCAC 
CTCTCTCGGG AGCGAGTCTT CACGGAGGAT CGGGCACGCT TTTATGGAGC AGAGATTGTG 
TCAGCTCTGG AGTACTTGCA CTCGAGGGAT GTGGTGTACC GCGACATCAA GCTGGAAAAC 
CTTATGCTGG ACAAAGATGG CCACATCAAG ATCACTGACT TTGGCTTGTG CAAAGAGGGC 
ATCAGTGATG GGGCCACCAT GAAAACCTTC TGTGGTACCC CGGAGTACTT GGCGCCTGAG 
GTGCTAGAGG ACAATGACTA CGGGCGTGCA GTGGACTGGT GGGGACTGGG CGTGGTCATG 
TACGAGATGA TGTGCGGCCG CCTGCCCTTC TACAACCAGG ACCACGAGCG CCTCTTCGAG 
CTCATCCTCA TGGAGGAGAT CCGGTTCCCA CGCACGCTTG GGCCAGAGGC CAAGTCCCTG 
CTGGCTGGAC TGCTCAAGAA GGACCCTAAG CAGAGGCTCG GTGGGGGTCC CAGTGATGCA 
AAGGAGGTCA TGGAGCATCG GTTCTTCCTC AGCATCAACT GGCAGGATGT GGTACAGAAG 
AAGCTCCTGC CACCCTTCAA ACCTCAGGTC ACTTCAGAAG TGGACACAAG GTACTTTGAT 
GATGAATTCA CCGCCCAGTC CATCACAATC ACACCCCCAG ACCGATATGA CAGCCTGGGC 
TCGCTGGAAC TGGACCAGCG CACACACTTC CCCCAGTTCT CCTACTCAGC CAGCATCCGA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Akt2-Dependent Beneficial Effect of Galanin on Insulin-Induced Glucose Uptake in Adipocytes of Diabetic Rats.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology41(5)1777-1787(2017)
Zhang Z,Fang P,Guo L,He B,Shi M,Zhu Y,Bo P

Activation of PI3K?/Akt pathway increases cardiomyocyte HMGB1 expression in diabetic environment.
Oncotarget7(49)80803-80810(2016 Dec)
Song J,Liu Q,Tang H,Tao A,Wang H,Kao R,Rui T

Inhibition of Akt2 phosphorylation abolishes the calorie restriction-induced improvement in insulin-stimulated glucose uptake by rat soleus muscle.
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme41(11)1208-1211(2016 Nov)
Sharma N,Arias EB,Cartee GD

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Akt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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