Pc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pc
Entrez Gene ID	25104
Full Name	pyruvate carboxylase
Synonyms	Pcx
General protein information	Preferred Names pyruvate carboxylase Names pyruvate carboxylase, mitochondrial PCB pyruvic carboxylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	biotin-containing enzyme that catalyses the carboxylation of pyruvate to form oxaloacetate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012744.2	NP_036876.2	pyruvate carboxylase, mitochondrial precursor
XM_006230691.3	XP_006230753.1	pyruvate carboxylase, mitochondrial isoform X2
XM_006230693.2	XP_006230755.1	pyruvate carboxylase, mitochondrial isoform X3
XM_006230692.3	XP_006230754.1	pyruvate carboxylase, mitochondrial isoform X3
XM_006230694.3	XP_006230756.1	pyruvate carboxylase, mitochondrial isoform X3
XM_006230689.3	XP_006230751.2	pyruvate carboxylase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PC	NP_071504.2
Canis lupus familiaris (dog)	PC	XP_540825.2
Bos taurus (cattle)	PC	NP_808815.2
Mus musculus (house mouse)	Pcx	NP_001156418.1
Caenorhabditis elegans (roundworm)	pyc-1	NP_001256376.1
Saccharomyces cerevisiae (baker's yeast)	PYC2	NP_009777.1
Kluyveromyces lactis	KLLA0C05764g	XP_452456.1
Eremothecium gossypii	AGOS_AAR162C	NP_982705.2
Magnaporthe oryzae (rice blast fungus)	MGG_07756	XP_003712969.1
Pan troglodytes (chimpanzee)	PC	XP_003952003.1
Neurospora crassa	NCU02505	XP_965636.1
Xenopus tropicalis (tropical clawed frog)	pc.2	NP_001096495.1
Schizosaccharomyces pombe (fission yeast)	pyr1	NP_595900.1
	PC	XP_001107814.1
Rattus norvegicus (Norway rat)	Pc	NP_036876.2
Danio rerio (zebrafish)	pcxb	NP_571625.1
Drosophila melanogaster (fruit fly)	CG1516	NP_724844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004742	XP_001689096.1
Saccharomyces cerevisiae (baker's yeast)	PYC1	NP_011453.1

Gene Ontology
Bibliography

Related articles in PubMed

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
Martin J, Balmer ML, Rajendran S, Maurhofer O, Dufour JF, St-Pierre MV
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 Apr)

Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element.
Wutthisathapornchai A, Vongpipatana T, Muangsawat S, Boonsaen T, MacDonald MJ, Jitrapakdee S
PloS one9(7)e102730(2014)

Knockdown of pyruvate carboxylase or fatty acid synthase lowers numerous lipids and glucose-stimulated insulin release in insulinoma cells.
MacDonald MJ, Hasan NM, Dobrzyn A, Stoker SW, Ntambi JM, Liu X, Sampath H
Archives of biochemistry and biophysics532(1)23-31(2013 Apr)

Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation.
Yim SA, Lim YS, Kim JW, Hwang SB
PloS one8(7)e68170(2013)

Glucose induces expression of rat pyruvate carboxylase through a carbohydrate response element in the distal gene promoter.
Pedersen KB, Buckley RS, Scioneaux R
The Biochemical journal426(2)159-70(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that glucose-mediated transcriptional activation of the pyruvate carboxylase (PC) gene is regulated by transcription factors.
Title: Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element.

Pyruvate carboxylase and fatty acid synthase were knocked down in insulin cells. Insulin secretion, phospholipid and cholesterol esters were lowered.
Title: Knockdown of pyruvate carboxylase or fatty acid synthase lowers numerous lipids and glucose-stimulated insulin release in insulinoma cells.

A carbohydrate response element in the distal gene promoter contributes to the glucose-mediated expression of pyruvate carboxylase.
Title: Glucose induces expression of rat pyruvate carboxylase through a carbohydrate response element in the distal gene promoter.

The PC pathway in beta cells may play a key role in pyruvate metabolism, insulin secretion and cell proliferation.
Title: The role of pyruvate carboxylase in insulin secretion and proliferation in rat pancreatic beta cells.

PC is important for insulin secretion stimulated by noncarbohydrate insulin secretagogues
Title: Impaired anaplerosis and insulin secretion in insulinoma cells caused by small interfering RNA-mediated suppression of pyruvate carboxylase.

The following Pc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa23699	XM_006230691.3 Latest version!	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa23700	XM_006230694.3 Latest version!	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa23700	XM_006230693.2 Latest version!	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa23700	XM_006230692.3 Latest version!	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
ORa56398	XM_006230689.3 Latest version!	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa12990	NM_012744.2 Latest version!	Rattus norvegicus pyruvate carboxylase (Pc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$867.30 $1239.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pc ( XM_006230691.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230753.1 Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa23699
Clone ID Related Accession (Same CDS sequence)	XM_006230691.3
Accession Version	XM_006230691.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCTGA AGTTCCAAAC AGTTCGAGGG GGCCTGAGGC TCCTGGGTGT CCGCCGATCC 
TCCACAGCCC CCGTTGCCTC CCCAAATGTC CGGCGTCTGG AGTACAAGCC CATCAAGAAA 
GTAATGGTGG CCAACAGAGG TGAGATTGCC ATCCGAGTGT TTCGTGCCTG CACAGAGCTG 
GGTATCCGCA CAGTGGCTGT CTACTCGGAG CAGGACACAG GCCAGATGCA CCGGCAGAAA 
GCTGATGAAG CCTACCTTAT TGGCCGTGGG CTGGCTCCTG TGCAAGCCTA CCTGCACATT 
CCAGACATCA TTAAGGTGGC CAAGGAGAAT GGTGTAGATG CTGTGCACCC TGGCTATGGG 
TTCCTCTCAG AGAGAGCAGA CTTTGCCCAG GCCTGCCAAG ATGCTGGAGT CCGATTCATT 
GGTCCAAGCC CAGAGGTGGT CCGCAAGATG GGAGACAAGG TGGAAGCCCG GGCCATTGCC 
ATTGCTGCAG GCGTTCCAGT GGTCCCTGGC ACTAATTCCC CCATCAATTC CCTGCATGAG 
GCACACGAGT TCTCTAACAC CTATGGTTTC CCTATTATCT TCAAGGCTGC CTATGGAGGT 
GGGGGCCGTG GCATGAGGGT TGTGCATAGC TACGAGGAGC TGGAAGAGAA TTACACCCGG 
GCCTACTCTG AGGCCTTGGC AGCCTTTGGG AATGGGGCAT TGTTTGTGGA GAAATTCATT 
GAGAAGCCAA GACACATTGA GGTGCAGATC CTAGGGGACC AATATGGGAA CATCTTGCAC 
TTGTATGAGC GGGACTGCTC CATCCAGCGG CGGCACCAGA AGGTGGTAGA GATTGCCCCT 
GCTACCCACC TGGACCCCCA ACTTCGGTCA CGCCTCACCA GTGACTCTGT CAAACTTGCC 
AAGCAGGTTG GCTATGAGAA TGCAGGCACT GTGGAGTTCC TGGTGGACAA GCATGGCAAG 
CACTACTTCA TCGAGGTCAA TTCCCGCCTG CAGGTGGAGC ACACGGTCAC TGAGGAGATT 
ACAGATGTGG ACCTGGTCCA TGCTCAGATC CATGTGTCCG AAGGCCGGAG CCTGCCTGAC 
CTAGGCCTGC GGCAGGAAAA CATCCGAATC AATGGTTGTG CCATTCAGTG TCGGGTCACC 
ACTGAGGACC CTGCACGCAG CTTCCAGCCA GACACTGGCC GCATTGAGGT TTTCCGGAGT 
GGTGAGGGCA TGGGCATCCG CCTGGACAAT GCCTCAGCAT TCCAGGGAGC TGTCATATCC 
CCCCACTATG ACTCCCTGCT CGTCAAAGTC ATTGCCCATG GCAAAGACCA CCCTACAGCT 
GCCACCAAGA TGAGCAGAGC CCTGGCGGAG TTCCGTGTCC GAGGTGTAAA GACCAACATC 
CCCTTCCTGC AGAATGTGCT CAACAACCAG CAGTTCCTAG CGGGCATTGT GGACACCCAG 
TTCATCGATG AGAACCCCGA GCTGTTCCAG CTGCGGCCTG CACAGAACCG GGCCCAGAAG 
TTGCTACATT ACCTTGGACA CGTCATGGTC AATGGCCCTA CCACTCCAAT CCCCGTCAAG 
GTCAGTCCCA GCCCTGTGGA CCCCATTGTT CCTGTGGTGC CCATAGGCCC ACCCCCAGCT 
GGTTTCAGAG ACATCCTTCT GCGAGAGGGG CCAGAGGGCT TTGCCAGAGC TGTGCGGAAT 
CACCAGGGGC TGCTGCTAAT GGACACAACC TTCCGGGATG CCCACCAGTC ACTACTTGCC 
ACTAGAGTGC GCACACACGA TCTCAAAAAG ATTGCACCCT ACGTTGCCCA CAACTTCAAC 
AACCTCTTCA GCATAGAGAA CTGGGGAGGA GCCACATTTG ACGTGGCCAT GCGCTTCTTG 
TATGAGTGCC CCTGGCGGCG GCTCCAGGAG CTCCGGGAGC TCATCCCCAA CATCCCATTC 
CAGATGCTAC TGAGGGGGGC CAATGCTGTG GGCTACACCA ACTACCCTGA CAACGTGGTC 
TTCAAGTTCT GTGAGGTGGC CAAAGAGAAT GGCATGGACG TCTTCCGGAT CTTTGACTCC 
CTTAACTACC TGCCAAACAT GCTGCTGGGC ATGGAAGCAG CTGGCAGTGC TGGGGGTGTG 
GTGGAAGCTG CCATCTCCTA CACGGGTGAC GTGGCTGACC CCAGTCGCAC TAAATACTCA 
CTGGAGTACT ACATGGGCTT AGCTGAAGAA CTGGTGCGAG CCGGCACTCA CATCCTCTGC 
ATTAAGGACA TGGCAGGCCT GCTGAAGCCT GCAGCATGCA CCATGCTGGT CAGCTCCCTC 
CGGGACCGGT TCCCCGACCT CCCACTGCAC ATCCATACCC ATGACACATC AGGGTCAGGT 
GTGGCAGCCA TGTTGGCCTG TGCACAAGCT GGGGCTGATG TTGTGGATGT GGCAGTCGAC 
TCTATGTCTG GGATGACCTC ACAGCCCAGC ATGGGGGCCC TGGTGGCCTG TACCAAAGGG 
ACTCCTCTGG ACACAGAGGT ACCCCTGGAG CGTGTGTTTG ACTACAGTGA GTATTGGGAA 
GGGGCTCGGG GGCTGTATGC AGCCTTTGAT TGCACGGCTA CCATGAAGTC TGGCAACTCA 
GACGTGTATG AGAATGAGAT CCCAGGGGGC CAGTACACCA ACCTACACTT CCAGGCCCAC 
AGCATGGGAC TTGGCTCCAA GTTCAAGGAG GTCAAGAAGG CCTATGTGGA GGCTAACCAG 
ATGCTGGGGG ACCTCATCAA GGTGACACCA TCCTCCAAGA TTGTGGGGGA TCTGGCCCAG 
TTCATGGTGC AGAACGGGTT GAGCCGGGCA GAGGCAGAAG CTCAGGCAGA AGAGCTGTCC 
TTCCCCCGCT CTGTGGTGGA GTTCCTGCAG GGCTACATTG GCATTCCCCA TGGGGGTTTC 
CCTGAACCCT TCCGTTCTAA GGTGCTAAAG GACCTGCCAA GAATAGAAGG GAGGCCTGGA 
GCCTCCCTCC CTCCCTTGAA CCTGAAGGAG CTGGAGAAGG ACCTGATTGA TAGGCATGGA 
GAGGAGGTGA CCCCAGAGGA CGTTCTCTCT GCAGCCATGT ACCCTGATGT CTTTGCTCAG 
TTCAAAGACT TCACGGCTAC CTTTGGCCCC CTGGATAGCC TGAATACTCG TCTCTTTCTT 
CAAGGACCCA AAATTGCAGA GGAGTTTGAG GTTGAGCTGG AACGGGGCAA GACCTTGCAC 
ATCAAAGCCC TGGCTGTAAG CGACCTGAAC CGTGCTGGCC AGAGGCAGGT GTTCTTTGAA 
CTCAATGGGC AGCTTCGATC CATTCTGGTT AAAGACACCC AGGCCATGAA GGAGATGCAC 
TTCCATCCCA AGGCCTTGAA GGATGTGAAG GGCCAAATTG GGGCCCCTAT GCCTGGGAAG 
GTCATAGACG TCAAGGTGGC AGCAGGAGCC AAGGTGGTTA AGGGCCAGCC CCTCTGTGTG 
CTCAGCGCCA TGAAGATGGA GACTGTGGTG ACTTCGCCCA TGGAGGGCAC TATCCGAAAG 
GTTCACGTGA CCAAGGACAT GACTCTGGAA GGCGATGACC TCATCCTAGA GATTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230753.1
CDS	127..3666
Translation	MMLKFQTVRGGLRLLGVRRSSTAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGIVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKVSPSPVDPIVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNNLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRIFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGSGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006230691.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230691.3

ORF Insert Sequence:

ATGATGCTGA AGTTCCAAAC AGTTCGAGGG GGCCTGAGGC TCCTGGGTGT CCGCCGATCC 
TCCACAGCCC CCGTTGCCTC CCCAAATGTC CGGCGTCTGG AGTACAAGCC CATCAAGAAA 
GTAATGGTGG CCAACAGAGG TGAGATTGCC ATCCGAGTGT TTCGTGCCTG CACAGAGCTG 
GGTATCCGCA CAGTGGCTGT CTACTCGGAG CAGGACACAG GCCAGATGCA CCGGCAGAAA 
GCTGATGAAG CCTACCTTAT TGGCCGTGGG CTGGCTCCTG TGCAAGCCTA CCTGCACATT 
CCAGACATCA TTAAGGTGGC CAAGGAGAAT GGTGTAGATG CTGTGCACCC TGGCTATGGG 
TTCCTCTCAG AGAGAGCAGA CTTTGCCCAG GCCTGCCAAG ATGCTGGAGT CCGATTCATT 
GGTCCAAGCC CAGAGGTGGT CCGCAAGATG GGAGACAAGG TGGAAGCCCG GGCCATTGCC 
ATTGCTGCAG GCGTTCCAGT GGTCCCTGGC ACTAATTCCC CCATCAATTC CCTGCATGAG 
GCACACGAGT TCTCTAACAC CTATGGTTTC CCTATTATCT TCAAGGCTGC CTATGGAGGT 
GGGGGCCGTG GCATGAGGGT TGTGCATAGC TACGAGGAGC TGGAAGAGAA TTACACCCGG 
GCCTACTCTG AGGCCTTGGC AGCCTTTGGG AATGGGGCAT TGTTTGTGGA GAAATTCATT 
GAGAAGCCAA GACACATTGA GGTGCAGATC CTAGGGGACC AATATGGGAA CATCTTGCAC 
TTGTATGAGC GGGACTGCTC CATCCAGCGG CGGCACCAGA AGGTGGTAGA GATTGCCCCT 
GCTACCCACC TGGACCCCCA ACTTCGGTCA CGCCTCACCA GTGACTCTGT CAAACTTGCC 
AAGCAGGTTG GCTATGAGAA TGCAGGCACT GTGGAGTTCC TGGTGGACAA GCATGGCAAG 
CACTACTTCA TCGAGGTCAA TTCCCGCCTG CAGGTGGAGC ACACGGTCAC TGAGGAGATT 
ACAGATGTGG ACCTGGTCCA TGCTCAGATC CATGTGTCCG AAGGCCGGAG CCTGCCTGAC 
CTAGGCCTGC GGCAGGAAAA CATCCGAATC AATGGTTGTG CCATTCAGTG TCGGGTCACC 
ACTGAGGACC CTGCACGCAG CTTCCAGCCA GACACTGGCC GCATTGAGGT TTTCCGGAGT 
GGTGAGGGCA TGGGCATCCG CCTGGACAAT GCCTCAGCAT TCCAGGGAGC TGTCATATCC 
CCCCACTATG ACTCCCTGCT CGTCAAAGTC ATTGCCCATG GCAAAGACCA CCCTACAGCT 
GCCACCAAGA TGAGCAGAGC CCTGGCGGAG TTCCGTGTCC GAGGTGTAAA GACCAACATC 
CCCTTCCTGC AGAATGTGCT CAACAACCAG CAGTTCCTAG CGGGCATTGT GGACACCCAG 
TTCATCGATG AGAACCCCGA GCTGTTCCAG CTGCGGCCTG CACAGAACCG GGCCCAGAAG 
TTGCTACATT ACCTTGGACA CGTCATGGTC AATGGCCCTA CCACTCCAAT CCCCGTCAAG 
GTCAGTCCCA GCCCTGTGGA CCCCATTGTT CCTGTGGTGC CCATAGGCCC ACCCCCAGCT 
GGTTTCAGAG ACATCCTTCT GCGAGAGGGG CCAGAGGGCT TTGCCAGAGC TGTGCGGAAT 
CACCAGGGGC TGCTGCTAAT GGACACAACC TTCCGGGATG CCCACCAGTC ACTACTTGCC 
ACTAGAGTGC GCACACACGA TCTCAAAAAG ATTGCACCCT ACGTTGCCCA CAACTTCAAC 
AACCTCTTCA GCATAGAGAA CTGGGGAGGA GCCACATTTG ACGTGGCCAT GCGCTTCTTG 
TATGAGTGCC CCTGGCGGCG GCTCCAGGAG CTCCGGGAGC TCATCCCCAA CATCCCATTC 
CAGATGCTAC TGAGGGGGGC CAATGCTGTG GGCTACACCA ACTACCCTGA CAACGTGGTC 
TTCAAGTTCT GTGAGGTGGC CAAAGAGAAT GGCATGGACG TCTTCCGGAT CTTTGACTCC 
CTTAACTACC TGCCAAACAT GCTGCTGGGC ATGGAAGCAG CTGGCAGTGC TGGGGGTGTG 
GTGGAAGCTG CCATCTCCTA CACGGGTGAC GTGGCTGACC CCAGTCGCAC TAAATACTCA 
CTGGAGTACT ACATGGGCTT AGCTGAAGAA CTGGTGCGAG CCGGCACTCA CATCCTCTGC 
ATTAAGGACA TGGCAGGCCT GCTGAAGCCT GCAGCATGCA CCATGCTGGT CAGCTCCCTC 
CGGGACCGGT TCCCCGACCT CCCACTGCAC ATCCATACCC ATGACACATC AGGGTCAGGT 
GTGGCAGCCA TGTTGGCCTG TGCACAAGCT GGGGCTGATG TTGTGGATGT GGCAGTCGAC 
TCTATGTCTG GGATGACCTC ACAGCCCAGC ATGGGGGCCC TGGTGGCCTG TACCAAAGGG 
ACTCCTCTGG ACACAGAGGT ACCCCTGGAG CGTGTGTTTG ACTACAGTGA GTATTGGGAA 
GGGGCTCGGG GGCTGTATGC AGCCTTTGAT TGCACGGCTA CCATGAAGTC TGGCAACTCA 
GACGTGTATG AGAATGAGAT CCCAGGGGGC CAGTACACCA ACCTACACTT CCAGGCCCAC 
AGCATGGGAC TTGGCTCCAA GTTCAAGGAG GTCAAGAAGG CCTATGTGGA GGCTAACCAG 
ATGCTGGGGG ACCTCATCAA GGTGACACCA TCCTCCAAGA TTGTGGGGGA TCTGGCCCAG 
TTCATGGTGC AGAACGGGTT GAGCCGGGCA GAGGCAGAAG CTCAGGCAGA AGAGCTGTCC 
TTCCCCCGCT CTGTGGTGGA GTTCCTGCAG GGCTACATTG GCATTCCCCA TGGGGGTTTC 
CCTGAACCCT TCCGTTCTAA GGTGCTAAAG GACCTGCCAA GAATAGAAGG GAGGCCTGGA 
GCCTCCCTCC CTCCCTTGAA CCTGAAGGAG CTGGAGAAGG ACCTGATTGA TAGGCATGGA 
GAGGAGGTGA CCCCAGAGGA CGTTCTCTCT GCAGCCATGT ACCCTGATGT CTTTGCTCAG 
TTCAAAGACT TCACGGCTAC CTTTGGCCCC CTGGATAGCC TGAATACTCG TCTCTTTCTT 
CAAGGACCCA AAATTGCAGA GGAGTTTGAG GTTGAGCTGG AACGGGGCAA GACCTTGCAC 
ATCAAAGCCC TGGCTGTAAG CGACCTGAAC CGTGCTGGCC AGAGGCAGGT GTTCTTTGAA 
CTCAATGGGC AGCTTCGATC CATTCTGGTT AAAGACACCC AGGCCATGAA GGAGATGCAC 
TTCCATCCCA AGGCCTTGAA GGATGTGAAG GGCCAAATTG GGGCCCCTAT GCCTGGGAAG 
GTCATAGACG TCAAGGTGGC AGCAGGAGCC AAGGTGGTTA AGGGCCAGCC CCTCTGTGTG 
CTCAGCGCCA TGAAGATGGA GACTGTGGTG ACTTCGCCCA TGGAGGGCAC TATCCGAAAG 
GTTCACGTGA CCAAGGACAT GACTCTGGAA GGCGATGACC TCATCCTAGA GATTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pc ( XM_006230693.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230755.1 Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa23700
Clone ID Related Accession (Same CDS sequence)	XM_006230693.2 , XM_006230692.3 , XM_006230694.3
Accession Version	XM_006230693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate carboxylase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006230693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTAGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTG GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTGTCCGAAG GCCGGAGCCT GCCTGACCTA 
GGCCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230755.1
CDS	222..3758
Translation	MLKFQTVRGGLRLLGVRRSSTAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGIVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKVSPSPVDPIVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNNLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRIFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGSGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006230693.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230693.2

ORF Insert Sequence:

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTAGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTG GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTGTCCGAAG GCCGGAGCCT GCCTGACCTA 
GGCCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pc ( XM_006230692.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230754.1 Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X3, mRNA.

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CloneID	ORa23700
Clone ID Related Accession (Same CDS sequence)	XM_006230693.2 , XM_006230692.3 , XM_006230694.3
Accession Version	XM_006230692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate carboxylase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTAGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTG GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTGTCCGAAG GCCGGAGCCT GCCTGACCTA 
GGCCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230754.1
CDS	91..3627
Translation	MLKFQTVRGGLRLLGVRRSSTAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGIVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKVSPSPVDPIVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNNLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRIFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGSGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006230692.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230692.3

ORF Insert Sequence:

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTAGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTG GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTGTCCGAAG GCCGGAGCCT GCCTGACCTA 
GGCCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pc ( XM_006230694.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230756.1 Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X5, mRNA.

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CloneID	ORa23700
Clone ID Related Accession (Same CDS sequence)	XM_006230693.2 , XM_006230692.3 , XM_006230694.3
Accession Version	XM_006230694.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate carboxylase, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230694.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTAGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTG GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTGTCCGAAG GCCGGAGCCT GCCTGACCTA 
GGCCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230756.1
CDS	91..3627
Translation	MLKFQTVRGGLRLLGVRRSSTAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGIVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKVSPSPVDPIVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNNLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRIFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGSGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006230694.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230694.3

ORF Insert Sequence:

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TGGTAGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTG GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTGTCCGAAG GCCGGAGCCT GCCTGACCTA 
GGCCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pc ( XM_006230689.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230751.2 Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X1, mRNA.

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CloneID	ORa56398
Clone ID Related Accession (Same CDS sequence)	XM_006230689.3
Accession Version	XM_006230689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230689.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTCGG GTGGAGCAGT TGCTGCCTGT GGATCCTCTA ACACGTCCTC TGTGTGCAGC 
ACGATTAGGT GTTTGGCTGC TGGTACAAGG TACATAGAGG AGGAGGCAAG CACACTGCAC 
CAATCTGGCA ATGAGGCGGT CCCCTGGCAT GATCTCACCT GTTGTCACCC TTGTTTCAGG 
AAGAAACTTC ATGAGACGAT GCTGAAGTTC CAAACAGTTC GAGGGGGCCT GAGGCTCCTG 
GGTGTCCGCC GATCCTCCAC AGCCCCCGTT GCCTCCCCAA ATGTCCGGCG TCTGGAGTAC 
AAGCCCATCA AGAAAGTAAT GGTGGCCAAC AGAGGTGAGA TTGCCATCCG AGTGTTTCGT 
GCCTGCACAG AGCTGGGTAT CCGCACAGTG GCTGTCTACT CGGAGCAGGA CACAGGCCAG 
ATGCACCGGC AGAAAGCTGA TGAAGCCTAC CTTATTGGCC GTGGGCTGGC TCCTGTGCAA 
GCCTACCTGC ACATTCCAGA CATCATTAAG GTGGCCAAGG AGAATGGTGT AGATGCTGTG 
CACCCTGGCT ATGGGTTCCT CTCAGAGAGA GCAGACTTTG CCCAGGCCTG CCAAGATGCT 
GGAGTCCGAT TCATTGGTCC AAGCCCAGAG GTGGTCCGCA AGATGGGAGA CAAGGTGGAA 
GCCCGGGCCA TTGCCATTGC TGCAGGCGTT CCAGTGGTCC CTGGCACTAA TTCCCCCATC 
AATTCCCTGC ATGAGGCACA CGAGTTCTCT AACACCTATG GTTTCCCTAT TATCTTCAAG 
GCTGCCTATG GAGGTGGGGG CCGTGGCATG AGGGTTGTGC ATAGCTACGA GGAGCTGGAA 
GAGAATTACA CCCGGGCCTA CTCTGAGGCC TTGGCAGCCT TTGGGAATGG GGCATTGTTT 
GTGGAGAAAT TCATTGAGAA GCCAAGACAC ATTGAGGTGC AGATCCTAGG GGACCAATAT 
GGGAACATCT TGCACTTGTA TGAGCGGGAC TGCTCCATCC AGCGGCGGCA CCAGAAGGTG 
GTAGAGATTG CCCCTGCTAC CCACCTGGAC CCCCAACTTC GGTCACGCCT CACCAGTGAC 
TCTGTCAAAC TTGCCAAGCA GGTTGGCTAT GAGAATGCAG GCACTGTGGA GTTCCTGGTG 
GACAAGCATG GCAAGCACTA CTTCATCGAG GTCAATTCCC GCCTGCAGGT GGAGCACACG 
GTCACTGAGG AGATTACAGA TGTGGACCTG GTCCATGCTC AGATCCATGT GTCCGAAGGC 
CGGAGCCTGC CTGACCTAGG CCTGCGGCAG GAAAACATCC GAATCAATGG TTGTGCCATT 
CAGTGTCGGG TCACCACTGA GGACCCTGCA CGCAGCTTCC AGCCAGACAC TGGCCGCATT 
GAGGTTTTCC GGAGTGGTGA GGGCATGGGC ATCCGCCTGG ACAATGCCTC AGCATTCCAG 
GGAGCTGTCA TATCCCCCCA CTATGACTCC CTGCTCGTCA AAGTCATTGC CCATGGCAAA 
GACCACCCTA CAGCTGCCAC CAAGATGAGC AGAGCCCTGG CGGAGTTCCG TGTCCGAGGT 
GTAAAGACCA ACATCCCCTT CCTGCAGAAT GTGCTCAACA ACCAGCAGTT CCTAGCGGGC 
ATTGTGGACA CCCAGTTCAT CGATGAGAAC CCCGAGCTGT TCCAGCTGCG GCCTGCACAG 
AACCGGGCCC AGAAGTTGCT ACATTACCTT GGACACGTCA TGGTCAATGG CCCTACCACT 
CCAATCCCCG TCAAGGTCAG TCCCAGCCCT GTGGACCCCA TTGTTCCTGT GGTGCCCATA 
GGCCCACCCC CAGCTGGTTT CAGAGACATC CTTCTGCGAG AGGGGCCAGA GGGCTTTGCC 
AGAGCTGTGC GGAATCACCA GGGGCTGCTG CTAATGGACA CAACCTTCCG GGATGCCCAC 
CAGTCACTAC TTGCCACTAG AGTGCGCACA CACGATCTCA AAAAGATTGC ACCCTACGTT 
GCCCACAACT TCAACAACCT CTTCAGCATA GAGAACTGGG GAGGAGCCAC ATTTGACGTG 
GCCATGCGCT TCTTGTATGA GTGCCCCTGG CGGCGGCTCC AGGAGCTCCG GGAGCTCATC 
CCCAACATCC CATTCCAGAT GCTACTGAGG GGGGCCAATG CTGTGGGCTA CACCAACTAC 
CCTGACAACG TGGTCTTCAA GTTCTGTGAG GTGGCCAAAG AGAATGGCAT GGACGTCTTC 
CGGATCTTTG ACTCCCTTAA CTACCTGCCA AACATGCTGC TGGGCATGGA AGCAGCTGGC 
AGTGCTGGGG GTGTGGTGGA AGCTGCCATC TCCTACACGG GTGACGTGGC TGACCCCAGT 
CGCACTAAAT ACTCACTGGA GTACTACATG GGCTTAGCTG AAGAACTGGT GCGAGCCGGC 
ACTCACATCC TCTGCATTAA GGACATGGCA GGCCTGCTGA AGCCTGCAGC ATGCACCATG 
CTGGTCAGCT CCCTCCGGGA CCGGTTCCCC GACCTCCCAC TGCACATCCA TACCCATGAC 
ACATCAGGGT CAGGTGTGGC AGCCATGTTG GCCTGTGCAC AAGCTGGGGC TGATGTTGTG 
GATGTGGCAG TCGACTCTAT GTCTGGGATG ACCTCACAGC CCAGCATGGG GGCCCTGGTG 
GCCTGTACCA AAGGGACTCC TCTGGACACA GAGGTACCCC TGGAGCGTGT GTTTGACTAC 
AGTGAGTATT GGGAAGGGGC TCGGGGGCTG TATGCAGCCT TTGATTGCAC GGCTACCATG 
AAGTCTGGCA ACTCAGACGT GTATGAGAAT GAGATCCCAG GGGGCCAGTA CACCAACCTA 
CACTTCCAGG CCCACAGCAT GGGACTTGGC TCCAAGTTCA AGGAGGTCAA GAAGGCCTAT 
GTGGAGGCTA ACCAGATGCT GGGGGACCTC ATCAAGGTGA CACCATCCTC CAAGATTGTG 
GGGGATCTGG CCCAGTTCAT GGTGCAGAAC GGGTTGAGCC GGGCAGAGGC AGAAGCTCAG 
GCAGAAGAGC TGTCCTTCCC CCGCTCTGTG GTGGAGTTCC TGCAGGGCTA CATTGGCATT 
CCCCATGGGG GTTTCCCTGA ACCCTTCCGT TCTAAGGTGC TAAAGGACCT GCCAAGAATA 
GAAGGGAGGC CTGGAGCCTC CCTCCCTCCC TTGAACCTGA AGGAGCTGGA GAAGGACCTG 
ATTGATAGGC ATGGAGAGGA GGTGACCCCA GAGGACGTTC TCTCTGCAGC CATGTACCCT 
GATGTCTTTG CTCAGTTCAA AGACTTCACG GCTACCTTTG GCCCCCTGGA TAGCCTGAAT 
ACTCGTCTCT TTCTTCAAGG ACCCAAAATT GCAGAGGAGT TTGAGGTTGA GCTGGAACGG 
GGCAAGACCT TGCACATCAA AGCCCTGGCT GTAAGCGACC TGAACCGTGC TGGCCAGAGG 
CAGGTGTTCT TTGAACTCAA TGGGCAGCTT CGATCCATTC TGGTTAAAGA CACCCAGGCC 
ATGAAGGAGA TGCACTTCCA TCCCAAGGCC TTGAAGGATG TGAAGGGCCA AATTGGGGCC 
CCTATGCCTG GGAAGGTCAT AGACGTCAAG GTGGCAGCAG GAGCCAAGGT GGTTAAGGGC 
CAGCCCCTCT GTGTGCTCAG CGCCATGAAG ATGGAGACTG TGGTGACTTC GCCCATGGAG 
GGCACTATCC GAAAGGTTCA CGTGACCAAG GACATGACTC TGGAAGGCGA TGACCTCATC 
CTAGAGATTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230751.2
CDS	50..3784
Translation	MTSGGAVAACGSSNTSSVCSTIRCLAAGTRYIEEEASTLHQSGNEAVPWHDLTCCHPCFRKKLHETMLKFQTVRGGLRLLGVRRSSTAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGIVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKVSPSPVDPIVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNNLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRIFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGSGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_006230689.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate carboxylase (Pc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230689.3

ORF Insert Sequence:

ATGACCTCGG GTGGAGCAGT TGCTGCCTGT GGATCCTCTA ACACGTCCTC TGTGTGCAGC 
ACGATTAGGT GTTTGGCTGC TGGTACAAGG TACATAGAGG AGGAGGCAAG CACACTGCAC 
CAATCTGGCA ATGAGGCGGT CCCCTGGCAT GATCTCACCT GTTGTCACCC TTGTTTCAGG 
AAGAAACTTC ATGAGACGAT GCTGAAGTTC CAAACAGTTC GAGGGGGCCT GAGGCTCCTG 
GGTGTCCGCC GATCCTCCAC AGCCCCCGTT GCCTCCCCAA ATGTCCGGCG TCTGGAGTAC 
AAGCCCATCA AGAAAGTAAT GGTGGCCAAC AGAGGTGAGA TTGCCATCCG AGTGTTTCGT 
GCCTGCACAG AGCTGGGTAT CCGCACAGTG GCTGTCTACT CGGAGCAGGA CACAGGCCAG 
ATGCACCGGC AGAAAGCTGA TGAAGCCTAC CTTATTGGCC GTGGGCTGGC TCCTGTGCAA 
GCCTACCTGC ACATTCCAGA CATCATTAAG GTGGCCAAGG AGAATGGTGT AGATGCTGTG 
CACCCTGGCT ATGGGTTCCT CTCAGAGAGA GCAGACTTTG CCCAGGCCTG CCAAGATGCT 
GGAGTCCGAT TCATTGGTCC AAGCCCAGAG GTGGTCCGCA AGATGGGAGA CAAGGTGGAA 
GCCCGGGCCA TTGCCATTGC TGCAGGCGTT CCAGTGGTCC CTGGCACTAA TTCCCCCATC 
AATTCCCTGC ATGAGGCACA CGAGTTCTCT AACACCTATG GTTTCCCTAT TATCTTCAAG 
GCTGCCTATG GAGGTGGGGG CCGTGGCATG AGGGTTGTGC ATAGCTACGA GGAGCTGGAA 
GAGAATTACA CCCGGGCCTA CTCTGAGGCC TTGGCAGCCT TTGGGAATGG GGCATTGTTT 
GTGGAGAAAT TCATTGAGAA GCCAAGACAC ATTGAGGTGC AGATCCTAGG GGACCAATAT 
GGGAACATCT TGCACTTGTA TGAGCGGGAC TGCTCCATCC AGCGGCGGCA CCAGAAGGTG 
GTAGAGATTG CCCCTGCTAC CCACCTGGAC CCCCAACTTC GGTCACGCCT CACCAGTGAC 
TCTGTCAAAC TTGCCAAGCA GGTTGGCTAT GAGAATGCAG GCACTGTGGA GTTCCTGGTG 
GACAAGCATG GCAAGCACTA CTTCATCGAG GTCAATTCCC GCCTGCAGGT GGAGCACACG 
GTCACTGAGG AGATTACAGA TGTGGACCTG GTCCATGCTC AGATCCATGT GTCCGAAGGC 
CGGAGCCTGC CTGACCTAGG CCTGCGGCAG GAAAACATCC GAATCAATGG TTGTGCCATT 
CAGTGTCGGG TCACCACTGA GGACCCTGCA CGCAGCTTCC AGCCAGACAC TGGCCGCATT 
GAGGTTTTCC GGAGTGGTGA GGGCATGGGC ATCCGCCTGG ACAATGCCTC AGCATTCCAG 
GGAGCTGTCA TATCCCCCCA CTATGACTCC CTGCTCGTCA AAGTCATTGC CCATGGCAAA 
GACCACCCTA CAGCTGCCAC CAAGATGAGC AGAGCCCTGG CGGAGTTCCG TGTCCGAGGT 
GTAAAGACCA ACATCCCCTT CCTGCAGAAT GTGCTCAACA ACCAGCAGTT CCTAGCGGGC 
ATTGTGGACA CCCAGTTCAT CGATGAGAAC CCCGAGCTGT TCCAGCTGCG GCCTGCACAG 
AACCGGGCCC AGAAGTTGCT ACATTACCTT GGACACGTCA TGGTCAATGG CCCTACCACT 
CCAATCCCCG TCAAGGTCAG TCCCAGCCCT GTGGACCCCA TTGTTCCTGT GGTGCCCATA 
GGCCCACCCC CAGCTGGTTT CAGAGACATC CTTCTGCGAG AGGGGCCAGA GGGCTTTGCC 
AGAGCTGTGC GGAATCACCA GGGGCTGCTG CTAATGGACA CAACCTTCCG GGATGCCCAC 
CAGTCACTAC TTGCCACTAG AGTGCGCACA CACGATCTCA AAAAGATTGC ACCCTACGTT 
GCCCACAACT TCAACAACCT CTTCAGCATA GAGAACTGGG GAGGAGCCAC ATTTGACGTG 
GCCATGCGCT TCTTGTATGA GTGCCCCTGG CGGCGGCTCC AGGAGCTCCG GGAGCTCATC 
CCCAACATCC CATTCCAGAT GCTACTGAGG GGGGCCAATG CTGTGGGCTA CACCAACTAC 
CCTGACAACG TGGTCTTCAA GTTCTGTGAG GTGGCCAAAG AGAATGGCAT GGACGTCTTC 
CGGATCTTTG ACTCCCTTAA CTACCTGCCA AACATGCTGC TGGGCATGGA AGCAGCTGGC 
AGTGCTGGGG GTGTGGTGGA AGCTGCCATC TCCTACACGG GTGACGTGGC TGACCCCAGT 
CGCACTAAAT ACTCACTGGA GTACTACATG GGCTTAGCTG AAGAACTGGT GCGAGCCGGC 
ACTCACATCC TCTGCATTAA GGACATGGCA GGCCTGCTGA AGCCTGCAGC ATGCACCATG 
CTGGTCAGCT CCCTCCGGGA CCGGTTCCCC GACCTCCCAC TGCACATCCA TACCCATGAC 
ACATCAGGGT CAGGTGTGGC AGCCATGTTG GCCTGTGCAC AAGCTGGGGC TGATGTTGTG 
GATGTGGCAG TCGACTCTAT GTCTGGGATG ACCTCACAGC CCAGCATGGG GGCCCTGGTG 
GCCTGTACCA AAGGGACTCC TCTGGACACA GAGGTACCCC TGGAGCGTGT GTTTGACTAC 
AGTGAGTATT GGGAAGGGGC TCGGGGGCTG TATGCAGCCT TTGATTGCAC GGCTACCATG 
AAGTCTGGCA ACTCAGACGT GTATGAGAAT GAGATCCCAG GGGGCCAGTA CACCAACCTA 
CACTTCCAGG CCCACAGCAT GGGACTTGGC TCCAAGTTCA AGGAGGTCAA GAAGGCCTAT 
GTGGAGGCTA ACCAGATGCT GGGGGACCTC ATCAAGGTGA CACCATCCTC CAAGATTGTG 
GGGGATCTGG CCCAGTTCAT GGTGCAGAAC GGGTTGAGCC GGGCAGAGGC AGAAGCTCAG 
GCAGAAGAGC TGTCCTTCCC CCGCTCTGTG GTGGAGTTCC TGCAGGGCTA CATTGGCATT 
CCCCATGGGG GTTTCCCTGA ACCCTTCCGT TCTAAGGTGC TAAAGGACCT GCCAAGAATA 
GAAGGGAGGC CTGGAGCCTC CCTCCCTCCC TTGAACCTGA AGGAGCTGGA GAAGGACCTG 
ATTGATAGGC ATGGAGAGGA GGTGACCCCA GAGGACGTTC TCTCTGCAGC CATGTACCCT 
GATGTCTTTG CTCAGTTCAA AGACTTCACG GCTACCTTTG GCCCCCTGGA TAGCCTGAAT 
ACTCGTCTCT TTCTTCAAGG ACCCAAAATT GCAGAGGAGT TTGAGGTTGA GCTGGAACGG 
GGCAAGACCT TGCACATCAA AGCCCTGGCT GTAAGCGACC TGAACCGTGC TGGCCAGAGG 
CAGGTGTTCT TTGAACTCAA TGGGCAGCTT CGATCCATTC TGGTTAAAGA CACCCAGGCC 
ATGAAGGAGA TGCACTTCCA TCCCAAGGCC TTGAAGGATG TGAAGGGCCA AATTGGGGCC 
CCTATGCCTG GGAAGGTCAT AGACGTCAAG GTGGCAGCAG GAGCCAAGGT GGTTAAGGGC 
CAGCCCCTCT GTGTGCTCAG CGCCATGAAG ATGGAGACTG TGGTGACTTC GCCCATGGAG 
GGCACTATCC GAAAGGTTCA CGTGACCAAG GACATGACTC TGGAAGGCGA TGACCTCATC 
CTAGAGATTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pc ( NM_012744.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036876.2 Rattus norvegicus pyruvate carboxylase (Pc), mRNA.

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CloneID	ORa12990
Clone ID Related Accession (Same CDS sequence)	NM_012744.2
Accession Version	NM_012744.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate carboxylase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U36585.1. On or before Jan 7, 2005 this sequence version replaced XM_346577.1, NM_012744.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U36585.1, U32314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TCGTCGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTC GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTCTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGGCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTGGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTCCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036876.2
CDS	67..3603
Translation	MLKFQTVRGGLRLLGVRRSSTAPVASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENGVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTNSPINSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEENYTRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPATHLDPQLRSRLTSDSVKLAKQVGYENAGTVEFLVDKHGKHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVSEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGIVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKVSPSPVDPIVPVVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFNNLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRIFDSLNYLPNMLLGMEAAGSAGGVVEAAISYTGDVADPSRTKYSLEYYMGLAEELVRAGTHILCIKDMAGLLKPAACTMLVSSLRDRFPDLPLHIHTHDTSGSGVAAMLACAQAGADVVDVAVDSMSGMTSQPSMGALVACTKGTPLDTEVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPFRSKVLKDLPRIEGRPGASLPPLNLKELEKDLIDRHGEEVTPEDVLSAAMYPDVFAQFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDVKVAAGAKVVKGQPLCVLSAMKMETVVTSPMEGTIRKVHVTKDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	NM_012744.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate carboxylase (Pc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012744.2

ORF Insert Sequence:

ATGCTGAAGT TCCAAACAGT TCGAGGGGGC CTGAGGCTCC TGGGTGTCCG CCGATCCTCC 
ACAGCCCCCG TTGCCTCCCC AAATGTCCGG CGTCTGGAGT ACAAGCCCAT CAAGAAAGTA 
ATGGTGGCCA ACAGAGGTGA GATTGCCATC CGAGTGTTTC GTGCCTGCAC AGAGCTGGGT 
ATCCGCACAG TGGCTGTCTA CTCGGAGCAG GACACAGGGC AGATGCACCG GCAGAAAGCT 
GATGAAGCCT ACCTTATTGG CCGTGGGCTG GCTCCTGTGC AAGCCTACCT GCACATTCCA 
GACATCATTA AGGTGGCCAA GGAGAATGGT GTAGATGCTG TGCACCCTGG CTATGGGTTC 
CTCTCAGAGA GAGCAGACTT TGCCCAGGCC TGCCAAGATG CTGGAGTCCG ATTCATTGGT 
CCAAGCCCAG AGGTGGTCCG CAAGATGGGA GACAAGGTGG AAGCCCGGGC CATTGCCATT 
GCTGCAGGCG TTCCAGTGGT CCCTGGCACT AATTCCCCCA TCAATTCCCT GCATGAGGCA 
CACGAGTTCT CTAACACCTA TGGTTTCCCT ATTATCTTCA AGGCTGCCTA TGGAGGTGGG 
GGCCGTGGCA TGAGGGTTGT GCATAGCTAC GAGGAGCTGG AAGAGAATTA CACCCGGGCC 
TACTCTGAGG CCTTGGCAGC CTTTGGGAAT GGGGCATTGT TTGTGGAGAA ATTCATTGAG 
AAGCCAAGAC ACATTGAGGT GCAGATCCTA GGGGACCAAT ATGGGAACAT CTTGCACTTG 
TATGAGCGGG ACTGCTCCAT CCAGCGGCGG CACCAGAAGG TCGTCGAGAT TGCCCCTGCT 
ACCCACCTGG ACCCCCAACT TCGGTCACGC CTCACCAGTG ACTCTGTCAA ACTTGCCAAG 
CAGGTTGGCT ATGAGAATGC AGGCACTGTC GAGTTCCTGG TGGACAAGCA TGGCAAGCAC 
TACTTCATCG AGGTCAATTC CCGCCTGCAG GTGGAGCACA CGGTCACTGA GGAGATTACA 
GATGTGGACC TGGTCCATGC TCAGATCCAT GTCTCCGAAG GCCGGAGCCT GCCTGACCTG 
GGGCTGCGGC AGGAAAACAT CCGAATCAAT GGTTGTGCCA TTCAGTGTCG GGTCACCACT 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGCA TTGAGGTTTT CCGGAGTGGT 
GAGGGCATGG GCATCCGCCT GGACAATGCC TCAGCATTCC AGGGAGCTGT CATATCCCCC 
CACTATGACT CCCTGCTGGT CAAAGTCATT GCCCATGGCA AAGACCACCC TACAGCTGCC 
ACCAAGATGA GCAGAGCCCT GGCGGAGTTC CGTGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTTCTCAA CAACCAGCAG TTCCTAGCGG GCATTGTGGA CACCCAGTTC 
ATCGATGAGA ACCCCGAGCT GTTCCAGCTG CGGCCTGCAC AGAACCGGGC CCAGAAGTTG 
CTACATTACC TTGGACACGT CATGGTCAAT GGCCCTACCA CTCCAATCCC CGTCAAGGTC 
AGTCCCAGCC CTGTGGACCC CATTGTTCCT GTGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTTCTGCG AGAGGGGCCA GAGGGCTTTG CCAGAGCTGT GCGGAATCAC 
CAGGGGCTGC TGCTAATGGA CACAACCTTC CGGGATGCCC ACCAGTCACT ACTTGCCACT 
AGAGTGCGCA CACACGATCT CAAAAAGATT GCACCCTACG TTGCCCACAA CTTCAACAAC 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACATTTGACG TGGCCATGCG CTTCTTGTAT 
GAGTGCCCCT GGCGGCGGCT CCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCATTCCAG 
ATGCTACTGA GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA CGTGGTCTTC 
AAGTTCTGTG AGGTGGCCAA AGAGAATGGC ATGGACGTCT TCCGGATCTT TGACTCCCTT 
AACTACCTGC CAAACATGCT GCTGGGCATG GAAGCAGCTG GCAGTGCTGG GGGTGTGGTG 
GAAGCTGCCA TCTCCTACAC GGGTGACGTG GCTGACCCCA GTCGCACTAA ATACTCACTG 
GAGTACTACA TGGGCTTAGC TGAAGAACTG GTGCGAGCCG GCACTCACAT CCTCTGCATT 
AAGGACATGG CAGGCCTGCT GAAGCCTGCA GCATGCACCA TGCTGGTCAG CTCCCTCCGG 
GACCGGTTCC CCGACCTCCC ACTGCACATC CATACCCATG ACACATCAGG GTCAGGTGTG 
GCAGCCATGT TGGCCTGTGC ACAAGCTGGG GCTGATGTTG TGGATGTGGC AGTCGACTCT 
ATGTCTGGGA TGACCTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGTAC CAAAGGGACT 
CCTCTGGACA CAGAGGTACC CCTGGAGCGT GTGTTTGACT ACAGTGAGTA TTGGGAAGGG 
GCTCGGGGGC TGTATGCAGC CTTTGATTGC ACGGCTACCA TGAAGTCTGG CAACTCAGAC 
GTGTATGAGA ATGAGATCCC AGGGGGCCAG TACACCAACC TACACTTCCA GGCCCACAGC 
ATGGGACTTG GCTCCAAGTT CAAGGAGGTC AAGAAGGCCT ATGTGGAGGC TAACCAGATG 
CTGGGGGACC TCATCAAGGT GACACCATCC TCCAAGATTG TGGGGGATCT GGCCCAGTTC 
ATGGTGCAGA ACGGGTTGAG CCGGGCAGAG GCAGAAGCTC AGGCAGAAGA GCTGTCCTTC 
CCCCGCTCTG TGGTGGAGTT CCTCCAGGGC TACATTGGCA TTCCCCATGG GGGTTTCCCT 
GAACCCTTCC GTTCTAAGGT GCTAAAGGAC CTGCCAAGAA TAGAAGGGAG GCCTGGAGCC 
TCCCTCCCTC CCTTGAACCT GAAGGAGCTG GAGAAGGACC TGATTGATAG GCATGGAGAG 
GAGGTGACCC CAGAGGACGT TCTCTCTGCA GCCATGTACC CTGATGTCTT TGCTCAGTTC 
AAAGACTTCA CGGCTACCTT TGGCCCCCTG GATAGCCTGA ATACTCGTCT CTTTCTTCAA 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTT GAGCTGGAAC GGGGCAAGAC CTTGCACATC 
AAAGCCCTGG CTGTAAGCGA CCTGAACCGT GCTGGCCAGA GGCAGGTGTT CTTTGAACTC 
AATGGGCAGC TTCGATCCAT TCTGGTTAAA GACACCCAGG CCATGAAGGA GATGCACTTC 
CATCCCAAGG CCTTGAAGGA TGTGAAGGGC CAAATTGGGG CCCCTATGCC TGGGAAGGTC 
ATAGACGTCA AGGTGGCAGC AGGAGCCAAG GTGGTTAAGG GCCAGCCCCT CTGTGTGCTC 
AGCGCCATGA AGATGGAGAC TGTGGTGACT TCGCCCATGG AGGGCACTAT CCGAAAGGTT 
CACGTGACCA AGGACATGAC TCTGGAAGGC GATGACCTCA TCCTAGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nutritional stress exacerbates hepatic steatosis induced by deletion of the histidine nucleotide-binding (Hint2) mitochondrial protein.
American journal of physiology. Gastrointestinal and liver physiology310(7)G497-509(2016 Apr)
Martin J,Balmer ML,Rajendran S,Maurhofer O,Dufour JF,St-Pierre MV

Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element.
PloS one9(7)e102730(2014)
Wutthisathapornchai A,Vongpipatana T,Muangsawat S,Boonsaen T,MacDonald MJ,Jitrapakdee S

Knockdown of pyruvate carboxylase or fatty acid synthase lowers numerous lipids and glucose-stimulated insulin release in insulinoma cells.
Archives of biochemistry and biophysics532(1)23-31(2013 Apr)
MacDonald MJ,Hasan NM,Dobrzyn A,Stoker SW,Ntambi JM,Liu X,Sampath H

Nonstructural 5A protein of hepatitis C virus interacts with pyruvate carboxylase and modulates viral propagation.
PloS one8(7)e68170(2013)
Yim SA,Lim YS,Kim JW,Hwang SB

Glucose induces expression of rat pyruvate carboxylase through a carbohydrate response element in the distal gene promoter.
The Biochemical journal426(2)159-70(2010 Feb)
Pedersen KB,Buckley RS,Scioneaux R

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Pc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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