Mitf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mitf
Entrez Gene ID	25094
Full Name	melanogenesis associated transcription factor
General protein information	Preferred Names melanogenesis associated transcription factor Names microphthalmia-associated transcription factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	basic helix-loop-helix-leucine zipper transcription factor; may play a role in osteoclast function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592477.1	XP_017447966.1	microphthalmia-associated transcription factor isoform X1
XM_017592478.1	XP_017447967.1	microphthalmia-associated transcription factor isoform X2
NM_001191089.2	NP_001178018.1	microphthalmia-associated transcription factor

Pathways from BioSystems

Homologies

Homo sapiens (human)	MITF	NP_937802.1
Canis lupus familiaris (dog)	MITF	NP_001003337.1
Xenopus tropicalis (tropical clawed frog)	mitf	NP_001093747.1
Pan troglodytes (chimpanzee)	MITF	XP_001138876.1
Bos taurus (cattle)	MITF	NP_001001150.1
Mus musculus (house mouse)	Mitf	NP_001106669.1
	MITF	XP_001086496.1
Gallus gallus (chicken)	MITF	NP_990360.1
Danio rerio (zebrafish)	mitfb	NP_571922.2
Rattus norvegicus (Norway rat)	Mitf	NP_001178018.1

Gene Ontology
Bibliography

Related articles in PubMed

Biallelic Mutations in MITF Cause Coloboma, Osteopetrosis, Microphthalmia, Macrocephaly, Albinism, and Deafness.
George A, Zand DJ, Hufnagel RB, Sharma R, Sergeev YV, Legare JM, Rice GM, Scott Schwoerer JA, Rius M, Tetri L, Gamm DM, Bharti K, Brooks BP
American journal of human genetics99(6)1388-1394(2016 Dec)

MITF interacts with the SWI/SNF subunit, BRG1, to promote GATA4 expression in cardiac hypertrophy.
Mehta G, Kumarasamy S, Wu J, Walsh A, Liu L, Williams K, Joe B, de la Serna IL
Journal of molecular and cellular cardiology88101-10(2015 Nov)

Identification of a ZEB2-MITF-ZEB1 transcriptional network that controls melanogenesis and melanoma progression.
Denecker G, Vandamme N, Akay O, Koludrovic D, Taminau J, Lemeire K, Gheldof A, De Craene B, Van Gele M, Brochez L, Udupi GM, Rafferty M, Balint B, Gallagher WM, Ghanem G, Huylebroeck D, Haigh J, van den Oord J, Larue L, Davidson I, Marine JC, Berx G
Cell death and differentiation21(8)1250-61(2014 Aug)

Strawberry notch homologue 2 regulates osteoclast fusion by enhancing the expression of DC-STAMP.
Maruyama K, Uematsu S, Kondo T, Takeuchi O, Martino MM, Kawasaki T, Akira S
The Journal of experimental medicine210(10)1947-60(2013 Sep)

Restricted leucine zipper dimerization and specificity of DNA recognition of the melanocyte master regulator MITF.
Pogenberg V, Ogmundsd?ttir MH, Bergsteinsd?ttir K, Schepsky A, Phung B, Deineko V, Milewski M, Steingr?msson E, Wilmanns M
Genes & development26(23)2647-58(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MITF interacts with BRG1 to promote GATA4 expression in cardiac hypertrophy.
Title: MITF interacts with the SWI/SNF subunit, BRG1, to promote GATA4 expression in cardiac hypertrophy.

We have characterized the biological role of a 23-aa domain derived from PIAS3 that induces apoptosis in these cells by inhibiting the transcriptional activity of both MITF and STAT3.
Title: A specific epitope of protein inhibitor of activated STAT3 is responsible for the induction of apoptosis in rat transformed mast cells.

The following Mitf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mitf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06862	NM_001191089.2 Latest version!	Rattus norvegicus melanocyte inducing transcription factor (Mitf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa60757	XM_017592477.1 Latest version!	Rattus norvegicus melanogenesis associated transcription factor (Mitf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa60758	XM_017592478.1 Latest version!	Rattus norvegicus melanogenesis associated transcription factor (Mitf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mitf ( NM_001191089.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178018.1 Rattus norvegicus melanocyte inducing transcription factor (Mitf), mRNA.

Price & Availability↑

CloneID	ORa06862
Clone ID Related Accession (Same CDS sequence)	NM_001191089.2
Accession Version	NM_001191089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Rattus norvegicus(Norway rat)
Product	microphthalmia-associated transcription factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01031746.1, AAHX01031749.1 and AAHX01031751.1. On Oct 7, 2014 this sequence version replaced NM_001191089.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the Celera genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCTG AATCGGGAAT CGTGGCGGAT TTCGAAGTCG GGGAGGAGTT TCACGAAGAA 
CCCAAAACCT ATTACGAACT CAAAAGTCAA CCTCTGAAGA GCAGCAGTTC TGCAGAGCAT 
TCTGGGGCCT CCAAGCCTCC GTTAAGCTCC TCCACTATGA CATCACGCAT CTTGCTACGC 
CAGCAACTCA TGCGTGAGCA GATGCAGGAG CAGGAGCGCA GGGAGCAGCA GCAGAAGCTG 
CAGGCAGCCC AGTTCATGCA ACAGAGAGTG GCCGTGAGTC AGACACCAGC CATAAACGTC 
AGCGTGCCCA CCACCCTTCC CTCTGCCACC CAGGTGCCGA TGGAAGTCCT CAAGGTGCAG 
ACCCACCTGG AAAACCCCAC CAAGTACCAC ATACAGCAAG CCCAGAGGCA CCAGGTAAAG 
CAGTACCTTT CTACCACTTT AGCAAACAAA CATGCCGGCC AAGTCCTGAG CCCGCCATGT 
CCAAACCAGC CTGGCGACCA TGCCATGCCT CCAGTGCCGG GGAGCAGCGC ACCCAACAGC 
CCCATGGCTA TGCTCACTCT TAACTCCAAC TGTGAAAAAG AGGCGTTTTA TAAGTTTGAG 
GAGCAGAGCA GGGCAGAGAG TGAGTGCCCA GGTATGAACA CGCACTCTCG AGCTTCGTGC 
ATGCAGATGG ATGATGTGAT TGATGACATC ATCAGCCTGG AATCAAGTTA TAATGAAGAA 
ATTTTGGGCT TGATGGATCC GGCCTTGCAA ATGGCAAATA CGTTACCTGT CTCTGGAAAC 
TTGATCGACC TATACAGCAA CCAGGGCCTG CCACCCCCAG GCCTTACCAT CAGCAACTCT 
TGTCCAGCCA ACCTTCCCAA CATAAAAAGA GAGCTCACAG CGTGTATTTT CCCCACAGAG 
TCTGAAGCAA GAGCATTGGC TAAAGAGAGG CAGAAAAAGG ACAATCACAA CTTGATTGAA 
CGGAGAAGAA GATTTAACAT AAATGACCGC ATTAAGGAAC TAGGTACTTT GATTCCCAAG 
TCAAACGATC CGGACATGCG TTGGAACAAG GGGACCATCC TCAAGGCCTC CGTGGACTAC 
ATCCGAAAGT TGCAACGGGA ACAGCAGCGA GCTAAGGACC TTGAAAACCG GCAGAAGAAG 
CTGGAGCATG CTAACCGCCA TCTGCTGCTC AGAGTGCAGG AGCTCGAGAT GCAGGCTAGA 
GCACATGGGC TTTCTCTTAT CCCATCCACG GGTCTCTGCT CGCCTGATTT GGTGAATCGG 
ATCATCAAGC AAGAACCAGT TCTTGAGAAC TGCAGCCAGG AACTTGTACA GCACCAGGCA 
GACCTGACAT GCACAACGAC TCTGGATCTC ACGGACGGTA CCATCTCCTT TACCAACAAC 
CTCGGCACAA TGCCTGAGAG CAGCCCGGCC TACAGCATCC CCAGGAAGAT GGCCTCCAAC 
TTGGAAGACA TCCTGATGGA CGATGCCCTC TCACCTGTCG GCGTCACCGA CCCACTGCTG 
TCATCAGTGT CCCCAGGAGC TTCGAAAACA AGCAGCCGGA GGAGCAGTAT GAGCGCAGAA 
GAAACGGAGC ATGCGTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178018.1
CDS	1..1581
Translation	MQSESGIVADFEVGEEFHEEPKTYYELKSQPLKSSSSAEHSGASKPPLSSSTMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVAVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRHQVKQYLSTTLANKHAGQVLSPPCPNQPGDHAMPPVPGSSAPNSPMAMLTLNSNCEKEAFYKFEEQSRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYSNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKDLENRQKKLEHANRHLLLRVQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQELVQHQADLTCTTTLDLTDGTISFTNNLGTMPESSPAYSIPRKMASNLEDILMDDALSPVGVTDPLLSSVSPGASKTSSRRSSMSAEETEHAC

Target ORF information:

RefSeq Version	NM_001191089.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus melanocyte inducing transcription factor (Mitf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191089.2

ORF Insert Sequence:

ATGCAGTCTG AATCGGGAAT CGTGGCGGAT TTCGAAGTCG GGGAGGAGTT TCACGAAGAA 
CCCAAAACCT ATTACGAACT CAAAAGTCAA CCTCTGAAGA GCAGCAGTTC TGCAGAGCAT 
TCTGGGGCCT CCAAGCCTCC GTTAAGCTCC TCCACTATGA CATCACGCAT CTTGCTACGC 
CAGCAACTCA TGCGTGAGCA GATGCAGGAG CAGGAGCGCA GGGAGCAGCA GCAGAAGCTG 
CAGGCAGCCC AGTTCATGCA ACAGAGAGTG GCCGTGAGTC AGACACCAGC CATAAACGTC 
AGCGTGCCCA CCACCCTTCC CTCTGCCACC CAGGTGCCGA TGGAAGTCCT CAAGGTGCAG 
ACCCACCTGG AAAACCCCAC CAAGTACCAC ATACAGCAAG CCCAGAGGCA CCAGGTAAAG 
CAGTACCTTT CTACCACTTT AGCAAACAAA CATGCCGGCC AAGTCCTGAG CCCGCCATGT 
CCAAACCAGC CTGGCGACCA TGCCATGCCT CCAGTGCCGG GGAGCAGCGC ACCCAACAGC 
CCCATGGCTA TGCTCACTCT TAACTCCAAC TGTGAAAAAG AGGCGTTTTA TAAGTTTGAG 
GAGCAGAGCA GGGCAGAGAG TGAGTGCCCA GGTATGAACA CGCACTCTCG AGCTTCGTGC 
ATGCAGATGG ATGATGTGAT TGATGACATC ATCAGCCTGG AATCAAGTTA TAATGAAGAA 
ATTTTGGGCT TGATGGATCC GGCCTTGCAA ATGGCAAATA CGTTACCTGT CTCTGGAAAC 
TTGATCGACC TATACAGCAA CCAGGGCCTG CCACCCCCAG GCCTTACCAT CAGCAACTCT 
TGTCCAGCCA ACCTTCCCAA CATAAAAAGA GAGCTCACAG CGTGTATTTT CCCCACAGAG 
TCTGAAGCAA GAGCATTGGC TAAAGAGAGG CAGAAAAAGG ACAATCACAA CTTGATTGAA 
CGGAGAAGAA GATTTAACAT AAATGACCGC ATTAAGGAAC TAGGTACTTT GATTCCCAAG 
TCAAACGATC CGGACATGCG TTGGAACAAG GGGACCATCC TCAAGGCCTC CGTGGACTAC 
ATCCGAAAGT TGCAACGGGA ACAGCAGCGA GCTAAGGACC TTGAAAACCG GCAGAAGAAG 
CTGGAGCATG CTAACCGCCA TCTGCTGCTC AGAGTGCAGG AGCTCGAGAT GCAGGCTAGA 
GCACATGGGC TTTCTCTTAT CCCATCCACG GGTCTCTGCT CGCCTGATTT GGTGAATCGG 
ATCATCAAGC AAGAACCAGT TCTTGAGAAC TGCAGCCAGG AACTTGTACA GCACCAGGCA 
GACCTGACAT GCACAACGAC TCTGGATCTC ACGGACGGTA CCATCTCCTT TACCAACAAC 
CTCGGCACAA TGCCTGAGAG CAGCCCGGCC TACAGCATCC CCAGGAAGAT GGCCTCCAAC 
TTGGAAGACA TCCTGATGGA CGATGCCCTC TCACCTGTCG GCGTCACCGA CCCACTGCTG 
TCATCAGTGT CCCCAGGAGC TTCGAAAACA AGCAGCCGGA GGAGCAGTAT GAGCGCAGAA 
GAAACGGAGC ATGCGTGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mitf ( XM_017592477.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447966.1 Rattus norvegicus melanogenesis associated transcription factor (Mitf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60757
Clone ID Related Accession (Same CDS sequence)	XM_017592477.1
Accession Version	XM_017592477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	microphthalmia-associated transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCTG AATCGGGAAT CGTGGCGGAT TTCGAAGTCG GGGAGGAGTT TCACGAAGAA 
CCCAAAACCT ATTACGAACT CAAAAGTCAA CCTCTGAAGA GCAGCAGTTC TGCAGAGCAT 
TCTGGGGCCT CCAAGCCTCC GTTAAGCTCC TCCACTATGA CATCACGCAT CTTGCTACGC 
CAGCAACTCA TGCGTGAGCA GATGCAGGAG CAGGAGCGCA GGGAGCAGCA GCAGAAGCTG 
CAGGCAGCCC AGTTCATGCA ACAGAGAGTG GCCGTGAGTC AGACACCAGC CATAAACGTC 
AGCGTGCCCA CCACCCTTCC CTCTGCCACC CAGGTGCCGA TGGAAGTCCT CAAGGTGCAG 
ACCCACCTGG AAAACCCCAC CAAGTACCAC ATACAGCAAG CCCAGAGGCA CCAGGTAAAG 
CAGTACCTTT CTACCACTTT AGCAAACAAA CATGCCGGCC AAGTCCTGAG CCCGCCATGT 
CCAAACCAGC CTGGCGACCA TGCCATGCCT CCAGTGCCGG GGAGCAGCGC ACCCAACAGC 
CCCATGGCTA TGCTCACTCT TAACTCCAAC TGTGAAAAAG AGGCGTTTTA TAAGTTTGAG 
GAGCAGAGCA GGGCAGAGAG TGAGTGCCCA GGTATGAACA CGCACTCTCG AGCTTCGTGC 
ATGCAGATGG ATGATGTGAT TGATGACATC ATCAGCCTGG AATCAAGTTA TAATGAAGAA 
ATTTTGGGCT TGATGGATCC GGCCTTGCAA ATGGCAAATA CGTTACCTGT CTCTGGAAAC 
TTGATCGACC TATACAGCAA CCAGGGCCTG CCACCCCCAG GCCTTACCAT CAGCAACTCT 
TGTCCAGCCA ACCTTCCCAA CATAAAAAGA GAGCTCACAG AGTCTGAAGC AAGAGCATTG 
GCTAAAGAGA GGCAGAAAAA GGACAATCAC AACTTGATTG AACGGAGAAG AAGATTTAAC 
ATAAATGACC GCATTAAGGA ACTAGGTACT TTGATTCCCA AGTCAAACGA TCCGGACATG 
CGTTGGAACA AGGGGACCAT CCTCAAGGCC TCCGTGGACT ACATCCGAAA GTTGCAACGG 
GAACAGCAGC GAGCTAAGGA CCTTGAAAAC CGGCAGAAGA AGCTGGAGCA TGCTAACCGC 
CATCTGCTGC TCAGAGTGCA GGAGCTCGAG ATGCAGGCTA GAGCACATGG GCTTTCTCTT 
ATCCCATCCA CGGGTCTCTG CTCGCCTGAT TTGGTGAATC GGATCATCAA GCAAGAACCA 
GTTCTTGAGA ACTGCAGCCA GGAACTTGTA CAGCACCAGG CAGACCTGAC ATGCACAACG 
ACTCTGGATC TCACGGACGG TACCATCTCC TTTACCAACA ACCTCGGCAC AATGCCTGAG 
AGCAGCCCGG CCTACAGCAT CCCCAGGAAG ATGGCCTCCA ACTTGGAAGA CATCCTGATG 
GACGATGCCC TCTCACCTGT CGGCGTCACC GACCCACTGC TGTCATCAGT GTCCCCAGGA 
GCTTCGAAAA CAAGCAGCCG GAGGAGCAGT ATGAGCGCAG AAGAAACGGA GCATGCGTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447966.1
CDS	373..1935
Translation	MQSESGIVADFEVGEEFHEEPKTYYELKSQPLKSSSSAEHSGASKPPLSSSTMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVAVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRHQVKQYLSTTLANKHAGQVLSPPCPNQPGDHAMPPVPGSSAPNSPMAMLTLNSNCEKEAFYKFEEQSRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYSNQGLPPPGLTISNSCPANLPNIKRELTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKDLENRQKKLEHANRHLLLRVQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQELVQHQADLTCTTTLDLTDGTISFTNNLGTMPESSPAYSIPRKMASNLEDILMDDALSPVGVTDPLLSSVSPGASKTSSRRSSMSAEETEHAC

Target ORF information:

RefSeq Version	XM_017592477.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus melanogenesis associated transcription factor (Mitf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592477.1

ORF Insert Sequence:

ATGCAGTCTG AATCGGGAAT CGTGGCGGAT TTCGAAGTCG GGGAGGAGTT TCACGAAGAA 
CCCAAAACCT ATTACGAACT CAAAAGTCAA CCTCTGAAGA GCAGCAGTTC TGCAGAGCAT 
TCTGGGGCCT CCAAGCCTCC GTTAAGCTCC TCCACTATGA CATCACGCAT CTTGCTACGC 
CAGCAACTCA TGCGTGAGCA GATGCAGGAG CAGGAGCGCA GGGAGCAGCA GCAGAAGCTG 
CAGGCAGCCC AGTTCATGCA ACAGAGAGTG GCCGTGAGTC AGACACCAGC CATAAACGTC 
AGCGTGCCCA CCACCCTTCC CTCTGCCACC CAGGTGCCGA TGGAAGTCCT CAAGGTGCAG 
ACCCACCTGG AAAACCCCAC CAAGTACCAC ATACAGCAAG CCCAGAGGCA CCAGGTAAAG 
CAGTACCTTT CTACCACTTT AGCAAACAAA CATGCCGGCC AAGTCCTGAG CCCGCCATGT 
CCAAACCAGC CTGGCGACCA TGCCATGCCT CCAGTGCCGG GGAGCAGCGC ACCCAACAGC 
CCCATGGCTA TGCTCACTCT TAACTCCAAC TGTGAAAAAG AGGCGTTTTA TAAGTTTGAG 
GAGCAGAGCA GGGCAGAGAG TGAGTGCCCA GGTATGAACA CGCACTCTCG AGCTTCGTGC 
ATGCAGATGG ATGATGTGAT TGATGACATC ATCAGCCTGG AATCAAGTTA TAATGAAGAA 
ATTTTGGGCT TGATGGATCC GGCCTTGCAA ATGGCAAATA CGTTACCTGT CTCTGGAAAC 
TTGATCGACC TATACAGCAA CCAGGGCCTG CCACCCCCAG GCCTTACCAT CAGCAACTCT 
TGTCCAGCCA ACCTTCCCAA CATAAAAAGA GAGCTCACAG AGTCTGAAGC AAGAGCATTG 
GCTAAAGAGA GGCAGAAAAA GGACAATCAC AACTTGATTG AACGGAGAAG AAGATTTAAC 
ATAAATGACC GCATTAAGGA ACTAGGTACT TTGATTCCCA AGTCAAACGA TCCGGACATG 
CGTTGGAACA AGGGGACCAT CCTCAAGGCC TCCGTGGACT ACATCCGAAA GTTGCAACGG 
GAACAGCAGC GAGCTAAGGA CCTTGAAAAC CGGCAGAAGA AGCTGGAGCA TGCTAACCGC 
CATCTGCTGC TCAGAGTGCA GGAGCTCGAG ATGCAGGCTA GAGCACATGG GCTTTCTCTT 
ATCCCATCCA CGGGTCTCTG CTCGCCTGAT TTGGTGAATC GGATCATCAA GCAAGAACCA 
GTTCTTGAGA ACTGCAGCCA GGAACTTGTA CAGCACCAGG CAGACCTGAC ATGCACAACG 
ACTCTGGATC TCACGGACGG TACCATCTCC TTTACCAACA ACCTCGGCAC AATGCCTGAG 
AGCAGCCCGG CCTACAGCAT CCCCAGGAAG ATGGCCTCCA ACTTGGAAGA CATCCTGATG 
GACGATGCCC TCTCACCTGT CGGCGTCACC GACCCACTGC TGTCATCAGT GTCCCCAGGA 
GCTTCGAAAA CAAGCAGCCG GAGGAGCAGT ATGAGCGCAG AAGAAACGGA GCATGCGTGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mitf ( XM_017592478.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447967.1 Rattus norvegicus melanogenesis associated transcription factor (Mitf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60758
Clone ID Related Accession (Same CDS sequence)	XM_017592478.1
Accession Version	XM_017592478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	microphthalmia-associated transcription factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGC TTAGATTTGA GATGCTCATT CCCCGCTCCT TTGAAAGCTT GTATCTCAGT 
TCTGCAGAGC ATTCTGGGGC CTCCAAGCCT CCGTTAAGCT CCTCCACTAT GACATCACGC 
ATCTTGCTAC GCCAGCAACT CATGCGTGAG CAGATGCAGG AGCAGGAGCG CAGGGAGCAG 
CAGCAGAAGC TGCAGGCAGC CCAGTTCATG CAACAGAGAG TGGCCGTGAG TCAGACACCA 
GCCATAAACG TCAGCGTGCC CACCACCCTT CCCTCTGCCA CCCAGGTGCC GATGGAAGTC 
CTCAAGGTGC AGACCCACCT GGAAAACCCC ACCAAGTACC ACATACAGCA AGCCCAGAGG 
CACCAGGTAA AGCAGTACCT TTCTACCACT TTAGCAAACA AACATGCCGG CCAAGTCCTG 
AGCCCGCCAT GTCCAAACCA GCCTGGCGAC CATGCCATGC CTCCAGTGCC GGGGAGCAGC 
GCACCCAACA GCCCCATGGC TATGCTCACT CTTAACTCCA ACTGTGAAAA AGAGGCGTTT 
TATAAGTTTG AGGAGCAGAG CAGGGCAGAG AGTGAGTGCC CAGGTATGAA CACGCACTCT 
CGAGCTTCGT GCATGCAGAT GGATGATGTG ATTGATGACA TCATCAGCCT GGAATCAAGT 
TATAATGAAG AAATTTTGGG CTTGATGGAT CCGGCCTTGC AAATGGCAAA TACGTTACCT 
GTCTCTGGAA ACTTGATCGA CCTATACAGC AACCAGGGCC TGCCACCCCC AGGCCTTACC 
ATCAGCAACT CTTGTCCAGC CAACCTTCCC AACATAAAAA GAGAGCTCAC AGCGTGTATT 
TTCCCCACAG AGTCTGAAGC AAGAGCATTG GCTAAAGAGA GGCAGAAAAA GGACAATCAC 
AACTTGATTG AACGGAGAAG AAGATTTAAC ATAAATGACC GCATTAAGGA ACTAGGTACT 
TTGATTCCCA AGTCAAACGA TCCGGACATG CGTTGGAACA AGGGGACCAT CCTCAAGGCC 
TCCGTGGACT ACATCCGAAA GTTGCAACGG GAACAGCAGC GAGCTAAGGA CCTTGAAAAC 
CGGCAGAAGA AGCTGGAGCA TGCTAACCGC CATCTGCTGC TCAGAGTGCA GGAGCTCGAG 
ATGCAGGCTA GAGCACATGG GCTTTCTCTT ATCCCATCCA CGGGTCTCTG CTCGCCTGAT 
TTGGTGAATC GGATCATCAA GCAAGAACCA GTTCTTGAGA ACTGCAGCCA GGAACTTGTA 
CAGCACCAGG CAGACCTGAC ATGCACAACG ACTCTGGATC TCACGGACGG TACCATCTCC 
TTTACCAACA ACCTCGGCAC AATGCCTGAG AGCAGCCCGG CCTACAGCAT CCCCAGGAAG 
ATGGCCTCCA ACTTGGAAGA CATCCTGATG GACGATGCCC TCTCACCTGT CGGCGTCACC 
GACCCACTGC TGTCATCAGT GTCCCCAGGA GCTTCGAAAA CAAGCAGCCG GAGGAGCAGT 
ATGAGCGCAG AAGAAACGGA GCATGCGTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447967.1
CDS	27..1559
Translation	METLRFEMLIPRSFESLYLSSAEHSGASKPPLSSSTMTSRILLRQQLMREQMQEQERREQQQKLQAAQFMQQRVAVSQTPAINVSVPTTLPSATQVPMEVLKVQTHLENPTKYHIQQAQRHQVKQYLSTTLANKHAGQVLSPPCPNQPGDHAMPPVPGSSAPNSPMAMLTLNSNCEKEAFYKFEEQSRAESECPGMNTHSRASCMQMDDVIDDIISLESSYNEEILGLMDPALQMANTLPVSGNLIDLYSNQGLPPPGLTISNSCPANLPNIKRELTACIFPTESEARALAKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPDMRWNKGTILKASVDYIRKLQREQQRAKDLENRQKKLEHANRHLLLRVQELEMQARAHGLSLIPSTGLCSPDLVNRIIKQEPVLENCSQELVQHQADLTCTTTLDLTDGTISFTNNLGTMPESSPAYSIPRKMASNLEDILMDDALSPVGVTDPLLSSVSPGASKTSSRRSSMSAEETEHAC

Target ORF information:

RefSeq Version	XM_017592478.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus melanogenesis associated transcription factor (Mitf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592478.1

ORF Insert Sequence:

ATGGAGACGC TTAGATTTGA GATGCTCATT CCCCGCTCCT TTGAAAGCTT GTATCTCAGT 
TCTGCAGAGC ATTCTGGGGC CTCCAAGCCT CCGTTAAGCT CCTCCACTAT GACATCACGC 
ATCTTGCTAC GCCAGCAACT CATGCGTGAG CAGATGCAGG AGCAGGAGCG CAGGGAGCAG 
CAGCAGAAGC TGCAGGCAGC CCAGTTCATG CAACAGAGAG TGGCCGTGAG TCAGACACCA 
GCCATAAACG TCAGCGTGCC CACCACCCTT CCCTCTGCCA CCCAGGTGCC GATGGAAGTC 
CTCAAGGTGC AGACCCACCT GGAAAACCCC ACCAAGTACC ACATACAGCA AGCCCAGAGG 
CACCAGGTAA AGCAGTACCT TTCTACCACT TTAGCAAACA AACATGCCGG CCAAGTCCTG 
AGCCCGCCAT GTCCAAACCA GCCTGGCGAC CATGCCATGC CTCCAGTGCC GGGGAGCAGC 
GCACCCAACA GCCCCATGGC TATGCTCACT CTTAACTCCA ACTGTGAAAA AGAGGCGTTT 
TATAAGTTTG AGGAGCAGAG CAGGGCAGAG AGTGAGTGCC CAGGTATGAA CACGCACTCT 
CGAGCTTCGT GCATGCAGAT GGATGATGTG ATTGATGACA TCATCAGCCT GGAATCAAGT 
TATAATGAAG AAATTTTGGG CTTGATGGAT CCGGCCTTGC AAATGGCAAA TACGTTACCT 
GTCTCTGGAA ACTTGATCGA CCTATACAGC AACCAGGGCC TGCCACCCCC AGGCCTTACC 
ATCAGCAACT CTTGTCCAGC CAACCTTCCC AACATAAAAA GAGAGCTCAC AGCGTGTATT 
TTCCCCACAG AGTCTGAAGC AAGAGCATTG GCTAAAGAGA GGCAGAAAAA GGACAATCAC 
AACTTGATTG AACGGAGAAG AAGATTTAAC ATAAATGACC GCATTAAGGA ACTAGGTACT 
TTGATTCCCA AGTCAAACGA TCCGGACATG CGTTGGAACA AGGGGACCAT CCTCAAGGCC 
TCCGTGGACT ACATCCGAAA GTTGCAACGG GAACAGCAGC GAGCTAAGGA CCTTGAAAAC 
CGGCAGAAGA AGCTGGAGCA TGCTAACCGC CATCTGCTGC TCAGAGTGCA GGAGCTCGAG 
ATGCAGGCTA GAGCACATGG GCTTTCTCTT ATCCCATCCA CGGGTCTCTG CTCGCCTGAT 
TTGGTGAATC GGATCATCAA GCAAGAACCA GTTCTTGAGA ACTGCAGCCA GGAACTTGTA 
CAGCACCAGG CAGACCTGAC ATGCACAACG ACTCTGGATC TCACGGACGG TACCATCTCC 
TTTACCAACA ACCTCGGCAC AATGCCTGAG AGCAGCCCGG CCTACAGCAT CCCCAGGAAG 
ATGGCCTCCA ACTTGGAAGA CATCCTGATG GACGATGCCC TCTCACCTGT CGGCGTCACC 
GACCCACTGC TGTCATCAGT GTCCCCAGGA GCTTCGAAAA CAAGCAGCCG GAGGAGCAGT 
ATGAGCGCAG AAGAAACGGA GCATGCGTGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Biallelic Mutations in MITF Cause Coloboma, Osteopetrosis, Microphthalmia, Macrocephaly, Albinism, and Deafness.
American journal of human genetics99(6)1388-1394(2016 Dec)
George A,Zand DJ,Hufnagel RB,Sharma R,Sergeev YV,Legare JM,Rice GM,Scott Schwoerer JA,Rius M,Tetri L,Gamm DM,Bharti K,Brooks BP

MITF interacts with the SWI/SNF subunit, BRG1, to promote GATA4 expression in cardiac hypertrophy.
Journal of molecular and cellular cardiology88101-10(2015 Nov)
Mehta G,Kumarasamy S,Wu J,Walsh A,Liu L,Williams K,Joe B,de la Serna IL

Identification of a ZEB2-MITF-ZEB1 transcriptional network that controls melanogenesis and melanoma progression.
Cell death and differentiation21(8)1250-61(2014 Aug)
Denecker G,Vandamme N,Akay O,Koludrovic D,Taminau J,Lemeire K,Gheldof A,De Craene B,Van Gele M,Brochez L,Udupi GM,Rafferty M,Balint B,Gallagher WM,Ghanem G,Huylebroeck D,Haigh J,van den Oord J,Larue L,Davidson I,Marine JC,Berx G

Strawberry notch homologue 2 regulates osteoclast fusion by enhancing the expression of DC-STAMP.
The Journal of experimental medicine210(10)1947-60(2013 Sep)
Maruyama K,Uematsu S,Kondo T,Takeuchi O,Martino MM,Kawasaki T,Akira S

Restricted leucine zipper dimerization and specificity of DNA recognition of the melanocyte master regulator MITF.
Genes & development26(23)2647-58(2012 Dec)
Pogenberg V,Ogmundsd?ttir MH,Bergsteinsd?ttir K,Schepsky A,Phung B,Deineko V,Milewski M,Steingr?msson E,Wilmanns M

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Mitf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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