Hk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hk1
Entrez Gene ID	25058
Full Name	hexokinase 1
Synonyms	HEXOKIN,HKI
General protein information	Preferred Names hexokinase 1 Names hexokinase-1 HK I brain form hexokinase hexokinase type I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20q11
Summary	a ATP:D-hexose 6-phosphotransferase involved in the phosphorylation of glucose to produce glucose-6-phosphate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012734.1	NP_036866.1	hexokinase-1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Hex-A	NP_524848.1
Saccharomyces cerevisiae (baker's yeast)	HXK2	NP_011261.1
Oryza sativa (rice)	Os05g0522500	NP_001056082.1
Xenopus tropicalis (tropical clawed frog)	hk1	NP_001096201.1
Homo sapiens (human)	HK1	NP_000179.2
Bos taurus (cattle)	HK1	NP_001012686.1
Magnaporthe oryzae (rice blast fungus)	MGG_09289	XP_003709938.1
	HK1	XP_001110396.2
Mus musculus (house mouse)	Hk1	NP_001139572.1
Gallus gallus (chicken)	HK1	NP_989432.1
Eremothecium gossypii	AGOS_AFR279C	NP_985826.1
Canis lupus familiaris (dog)	HK1	XP_536376.3
Rattus norvegicus (Norway rat)	Hk1	NP_036866.1
Kluyveromyces lactis	KLLA0D11352g	XP_453567.1
Arabidopsis thaliana (thale cress)	HXK2	NP_179576.1
Oryza sativa (rice)	Os01g0742500	NP_001044214.1
Pan troglodytes (chimpanzee)	HK1	XP_001169264.2
Danio rerio (zebrafish)	hk1	NP_998417.1
Drosophila melanogaster (fruit fly)	Hex-t2	NP_733151.2
Drosophila melanogaster (fruit fly)	Hex-C	NP_524674.1
Saccharomyces cerevisiae (baker's yeast)	HXK1	NP_116711.3
Schizosaccharomyces pombe (fission yeast)	hxk1	NP_592948.1
Neurospora crassa	NCU02542	XP_965673.2
Arabidopsis thaliana (thale cress)	HXK1	NP_194642.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondria-Bound Hexokinase (mt-HK) Activity Differ in Cortical and Hypothalamic Synaptosomes: Differential Role of mt-HK in H
Cavalcanti-de-Albuquerque JP, de Souza Ferreira E, de Carvalho DP, Galina A
Molecular neurobiology(2017 Nov)

In-silico and in-vitro investigation on the phenylalanine metabolites' interactions with hexokinase of Rat's brain mitochondria.
Ziamajidi N, Jamshidi S, Ehsani-Zonouz A
Journal of bioenergetics and biomembranes49(2)139-147(2017 Apr)

c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis.
Zhang J, Wang S, Jiang B, Huang L, Ji Z, Li X, Zhou H, Han A, Chen A, Wu Y, Ma H, Zhao W, Zhao Q, Xie C, Sun X, Zhou Y, Huang H, Suleman M, Lin F, Zhou L, Tian F, Jin M, Cai Y, Zhang N, Li Q
Nature communications813732(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that phenylpyruvic acid is the cause of the reduction of glucose consumption by decreasing hexokinase activity. Therefore, production of ATP declines in brain cells.
Title: In-silico and in-vitro investigation on the phenylalanine metabolites' interactions with hexokinase of Rat's brain mitochondria.

There was an increase in hexokinase1 expression in right ventricle from pulmonary hypertension rats.
Title: Up-regulation of hexokinase1 in the right ventricle of monocrotaline induced pulmonary hypertension.

The results show that Hk1 and GLUT3 may play a role in the pathophysiology of ketamine-induced behavioral abnormalities.
Title: The expression of genes involved in glucose metabolism is affected by N-methyl-D-aspartate receptor antagonism: a putative link between metabolism and an animal model of psychosis.

Data show statistically significant altered levels of Tpi1 and alteration of Hk1, Aldoc, Pgam1 and Gapdh with borderline significance in phencyclidine (PCP) rats compared to controls.
Title: The application of selective reaction monitoring confirms dysregulation of glycolysis in a preclinical model of schizophrenia.

HKI remains strongly bound to mitochondria, whereas HKII translocates between mitochondria and the cytosol in response to glucose, G-6-P and Akt, but not ATP.
Title: Subcellular localization of hexokinases I and II directs the metabolic fate of glucose.

The following Hk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12868	NM_012734.1 Latest version!	Rattus norvegicus hexokinase 1 (Hk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hk1 ( NM_012734.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036866.1 Rattus norvegicus hexokinase 1 (Hk1), mRNA.

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CloneID	ORa12868
Clone ID Related Accession (Same CDS sequence)	NM_012734.1
Accession Version	NM_012734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	hexokinase-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J04526.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J04526.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01906347, SAMN01906348 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGATCGCCG CGCAACTACT GGCCTATTAC TTCACCGAGC TGAAGGATGA CCAAGTCAAA 
AAGATTGACA AGTATCTGTA CGCCATGCGG CTCTCTGATG AGATTCTGAT AGATATCCTG 
ACACGATTCA AGAAAGAGAT GAAGAATGGC CTCTCCCGGG ATTATAATCC AACAGCCTCC 
GTCAAGATGC TGCCCACCTT CGTCCGGTCC ATTCCGGACG GCTCAGAAAA GGGGGATTTC 
ATTGCCCTGG ATCTCGGCGG GTCTTCCTTT CGAATCCTGC GGGTGCAGGT GAACCACGAG 
AAGAACCAGA ACGTCAGCAT GGAGTCTGAG ATCTACGACA CCCCAGAGAA CATCGTGCAT 
GGCAGTGGAA CCCAGCTTTT CGATCATGTC GCTGACTGCC TGGGAGACTT CATGGAGAAA 
AAGAAGATCA AGGACAAGAA GTTACCCGTG GGATTCACAT TTTCCTTCCC CTGCCGACAA 
TCCAAGATAG ATGAGGCTGT ACTGATCACG TGGACAAAGC GGTTCAAAGC CAGTGGCGTG 
GAAGGAGCGG ATGTGGTCAA GTTGCTGAAT AAAGCCATTA AGAAGCGAGG GGACTATGAT 
GCTAACATTG TCGCCGTGGT GAATGACACA GTAGGGACCA TGATGACCTG CGGTTATGAT 
GACCAACAGT GTGAAGTCGG CCTGATCATT GGCACAGGCA CCAATGCTTG CTACATGGAG 
GAACTGCGAC ACATCGACCT GGTGGAAGGC GACGAGGGGA GGATGTGTAT TAACACGGAA 
TGGGGAGCCT TTGGGGATGA TGGGTCCCTG GAAGACATCC GAACCGAGTT TGACAGAGAG 
TTAGACCGTG GATCTCTCAA CCCTGGGAAG CAGCTGTTCG AGAAGATGGT GAGCGGCATG 
TACATGGGGG AGCTGGTCCG GCTAATCCTG GTGAAGATGG CCAAGGAAGG CCTCTTATTC 
GAAGGGCGCA TCACTCCAGA GCTGCTCACG AGGGGAAAGT TCAACACTAG TGACGTGTCC 
GCCATTGAAA AGGATAAGGA AGGCATTCAA AATGCCAAGG AAATCTTAAC CCGCTTGGGA 
GTGGAGCCGT CTGATGTTGA CTGTGTGTCG GTCCAGCACA TCTGCACGAT CGTCTCCTTC 
CGATCAGCCA ACCTGGTGGC CGCCACGCTC GGTGCCATCT TGAACCGCCT GCGGGACAAC 
AAGGGCACAC CACGCCTGCG GACCACGGTT GGCGTGGACG GTTCTCTCTA CAAGATGCAC 
CCACAGTACT CCCGGCGGTT CCACAAGACC CTGAGGCGCC TGGTGCCTGA CTCCGACGTC 
CGTTTCCTCC TCTCAGAGAG TGGCACGGGC AAGGGGGCCG CCATGGTGAC GGCAGTAGCC 
TACCGCCTGG CTGAGCAGCA CCGGCAGATT GAGGAAACCC TGGCCCACTT CCGCCTCAGC 
AAGCAGACGC TGATGGAGGT GAAGAAGAGG CTACGGACAG AGATGGAAAT GGGGCTGAGG 
AAGGAGACCA ACAGCAAAGC TACTGTCAAA ATGCTGCCTT CTTTTGTCCG GAGCATCCCG 
GATGGGACTG AACACGGTGA CTTCCTGGCC TTGGATCTTG GAGGAACGAA TTTCCGGGTT 
CTGCTGGTAA AGATCCGCAG TGGGAAAAAG AGAACAGTGG AAATGCACAA CAAGATCTAC 
TCCATTCCCC TGGAAATCAT GCAGGGCACC GGGGATGAGC TGTTTGACCA CATCGTCTCC 
TGCATCTCTG ACTTCCTGGA CTACATGGGG ATCAAAGGCC CCCGGATGCC TCTGGGCTTC 
ACCTTCTCAT TTCCCTGCCA TCAGACGAAC CTGGACTGTG GAATCTTGAT CTCATGGACA 
AAGGGTTTCA AAGCCACTGA CTGTGAGGGC CATGATGTAG CCTCCTTACT GAGGGATGCG 
GTGAAGAGGA GAGAGGAATT TGACTTGGAT GTGGTGGCTG TGGTCAACGA CACCGTGGGC 
ACCATGATGA CCTGTGCGTA TGAAGAACCC ACTTGCGAAA TTGGACTCAT CGTGGGGACG 
GGCACCAATG CCTGCTACAT GGAGGAGATG AAGAATGTGG AGATGGTGGA GGGGAACCAG 
GGCCAGATGT GCATCAACAT GGAGTGGGGC GCCTTCGGTG ACAATGGGTG TCTGGATGAC 
ATCAGAACAG ACTTTGACAA AGTGGTGGAC GAATATTCTC TAAACTCTGG GAAACAAAGG 
TTTGAGAAAA TGATCAGTGG GATGTACCTG GGTGAGATCG TCCGTAACAT CCTGATTGAC 
TTCACCAAGA AAGGCTTCCT CTTCCGGGGA CAGATCTCCG AACCACTCAA GACCCGAGGC 
ATCTTTGAGA CCAAGTTTCT CTCTCAGATT GAGAGTGACC GGTTAGCGCT GCTCCAGGTG 
CGGGCCATCC TTCAGCAGCT GGGTTTGAAC AGCACGTGTG ACGACAGTAT CCTGGTCAAG 
ACCGTGTGTG GGGTGGTGTC CAAGAGGGCG GCTCAGCTGT GTGGTGCCGG CATGGCCGCC 
GTGGTGGAAA AGATCAGAGA GAACAGAGGC CTAGACCATC TGAATGTAAC TGTGGGAGTG 
GATGGGACGC TCTACAAACT TCATCCACAC TTCTCCAGAA TCATGCACCA AACTGTGAAG 
GAACTGTCAC CAAAGTGTAC CGTGTCCTTC CTCCTGTCTG AAGACGGCAG CGGCAAGGGG 
GCCGCCCTTA TCACAGCTGT GGGCGTGCGG CTCAGAGGAG ACCCTTCGAT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036866.1
CDS	92..2848
Translation	MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDEILIDILTRFKKEMKNGLSRDYNPTASVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVSMESEIYDTPENIVHGSGTQLFDHVADCLGDFMEKKKIKDKKLPVGFTFSFPCRQSKIDEAVLITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDRELDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKDKEGIQNAKEILTRLGVEPSDVDCVSVQHICTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKMHPQYSRRFHKTLRRLVPDSDVRFLLSESGTGKGAAMVTAVAYRLAEQHRQIEETLAHFRLSKQTLMEVKKRLRTEMEMGLRKETNSKATVKMLPSFVRSIPDGTEHGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYSIPLEIMQGTGDELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCHQTNLDCGILISWTKGFKATDCEGHDVASLLRDAVKRREEFDLDVVAVVNDTVGTMMTCAYEEPTCEIGLIVGTGTNACYMEEMKNVEMVEGNQGQMCINMEWGAFGDNGCLDDIRTDFDKVVDEYSLNSGKQRFEKMISGMYLGEIVRNILIDFTKKGFLFRGQISEPLKTRGIFETKFLSQIESDRLALLQVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVEKIRENRGLDHLNVTVGVDGTLYKLHPHFSRIMHQTVKELSPKCTVSFLLSEDGSGKGAALITAVGVRLRGDPSIA

Target ORF information:

RefSeq Version	NM_012734.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hexokinase 1 (Hk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012734.1

ORF Insert Sequence:

ATGATCGCCG CGCAACTACT GGCCTATTAC TTCACCGAGC TGAAGGATGA CCAAGTCAAA 
AAGATTGACA AGTATCTGTA CGCCATGCGG CTCTCTGATG AGATTCTGAT AGATATCCTG 
ACACGATTCA AGAAAGAGAT GAAGAATGGC CTCTCCCGGG ATTATAATCC AACAGCCTCC 
GTCAAGATGC TGCCCACCTT CGTCCGGTCC ATTCCGGACG GCTCAGAAAA GGGGGATTTC 
ATTGCCCTGG ATCTCGGCGG GTCTTCCTTT CGAATCCTGC GGGTGCAGGT GAACCACGAG 
AAGAACCAGA ACGTCAGCAT GGAGTCTGAG ATCTACGACA CCCCAGAGAA CATCGTGCAT 
GGCAGTGGAA CCCAGCTTTT CGATCATGTC GCTGACTGCC TGGGAGACTT CATGGAGAAA 
AAGAAGATCA AGGACAAGAA GTTACCCGTG GGATTCACAT TTTCCTTCCC CTGCCGACAA 
TCCAAGATAG ATGAGGCTGT ACTGATCACG TGGACAAAGC GGTTCAAAGC CAGTGGCGTG 
GAAGGAGCGG ATGTGGTCAA GTTGCTGAAT AAAGCCATTA AGAAGCGAGG GGACTATGAT 
GCTAACATTG TCGCCGTGGT GAATGACACA GTAGGGACCA TGATGACCTG CGGTTATGAT 
GACCAACAGT GTGAAGTCGG CCTGATCATT GGCACAGGCA CCAATGCTTG CTACATGGAG 
GAACTGCGAC ACATCGACCT GGTGGAAGGC GACGAGGGGA GGATGTGTAT TAACACGGAA 
TGGGGAGCCT TTGGGGATGA TGGGTCCCTG GAAGACATCC GAACCGAGTT TGACAGAGAG 
TTAGACCGTG GATCTCTCAA CCCTGGGAAG CAGCTGTTCG AGAAGATGGT GAGCGGCATG 
TACATGGGGG AGCTGGTCCG GCTAATCCTG GTGAAGATGG CCAAGGAAGG CCTCTTATTC 
GAAGGGCGCA TCACTCCAGA GCTGCTCACG AGGGGAAAGT TCAACACTAG TGACGTGTCC 
GCCATTGAAA AGGATAAGGA AGGCATTCAA AATGCCAAGG AAATCTTAAC CCGCTTGGGA 
GTGGAGCCGT CTGATGTTGA CTGTGTGTCG GTCCAGCACA TCTGCACGAT CGTCTCCTTC 
CGATCAGCCA ACCTGGTGGC CGCCACGCTC GGTGCCATCT TGAACCGCCT GCGGGACAAC 
AAGGGCACAC CACGCCTGCG GACCACGGTT GGCGTGGACG GTTCTCTCTA CAAGATGCAC 
CCACAGTACT CCCGGCGGTT CCACAAGACC CTGAGGCGCC TGGTGCCTGA CTCCGACGTC 
CGTTTCCTCC TCTCAGAGAG TGGCACGGGC AAGGGGGCCG CCATGGTGAC GGCAGTAGCC 
TACCGCCTGG CTGAGCAGCA CCGGCAGATT GAGGAAACCC TGGCCCACTT CCGCCTCAGC 
AAGCAGACGC TGATGGAGGT GAAGAAGAGG CTACGGACAG AGATGGAAAT GGGGCTGAGG 
AAGGAGACCA ACAGCAAAGC TACTGTCAAA ATGCTGCCTT CTTTTGTCCG GAGCATCCCG 
GATGGGACTG AACACGGTGA CTTCCTGGCC TTGGATCTTG GAGGAACGAA TTTCCGGGTT 
CTGCTGGTAA AGATCCGCAG TGGGAAAAAG AGAACAGTGG AAATGCACAA CAAGATCTAC 
TCCATTCCCC TGGAAATCAT GCAGGGCACC GGGGATGAGC TGTTTGACCA CATCGTCTCC 
TGCATCTCTG ACTTCCTGGA CTACATGGGG ATCAAAGGCC CCCGGATGCC TCTGGGCTTC 
ACCTTCTCAT TTCCCTGCCA TCAGACGAAC CTGGACTGTG GAATCTTGAT CTCATGGACA 
AAGGGTTTCA AAGCCACTGA CTGTGAGGGC CATGATGTAG CCTCCTTACT GAGGGATGCG 
GTGAAGAGGA GAGAGGAATT TGACTTGGAT GTGGTGGCTG TGGTCAACGA CACCGTGGGC 
ACCATGATGA CCTGTGCGTA TGAAGAACCC ACTTGCGAAA TTGGACTCAT CGTGGGGACG 
GGCACCAATG CCTGCTACAT GGAGGAGATG AAGAATGTGG AGATGGTGGA GGGGAACCAG 
GGCCAGATGT GCATCAACAT GGAGTGGGGC GCCTTCGGTG ACAATGGGTG TCTGGATGAC 
ATCAGAACAG ACTTTGACAA AGTGGTGGAC GAATATTCTC TAAACTCTGG GAAACAAAGG 
TTTGAGAAAA TGATCAGTGG GATGTACCTG GGTGAGATCG TCCGTAACAT CCTGATTGAC 
TTCACCAAGA AAGGCTTCCT CTTCCGGGGA CAGATCTCCG AACCACTCAA GACCCGAGGC 
ATCTTTGAGA CCAAGTTTCT CTCTCAGATT GAGAGTGACC GGTTAGCGCT GCTCCAGGTG 
CGGGCCATCC TTCAGCAGCT GGGTTTGAAC AGCACGTGTG ACGACAGTAT CCTGGTCAAG 
ACCGTGTGTG GGGTGGTGTC CAAGAGGGCG GCTCAGCTGT GTGGTGCCGG CATGGCCGCC 
GTGGTGGAAA AGATCAGAGA GAACAGAGGC CTAGACCATC TGAATGTAAC TGTGGGAGTG 
GATGGGACGC TCTACAAACT TCATCCACAC TTCTCCAGAA TCATGCACCA AACTGTGAAG 
GAACTGTCAC CAAAGTGTAC CGTGTCCTTC CTCCTGTCTG AAGACGGCAG CGGCAAGGGG 
GCCGCCCTTA TCACAGCTGT GGGCGTGCGG CTCAGAGGAG ACCCTTCGAT CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondria-Bound Hexokinase (mt-HK) Activity Differ in Cortical and Hypothalamic Synaptosomes: Differential Role of mt-HK in H
Molecular neurobiology(2017 Nov)
Cavalcanti-de-Albuquerque JP,de Souza Ferreira E,de Carvalho DP,Galina A

In-silico and in-vitro investigation on the phenylalanine metabolites' interactions with hexokinase of Rat's brain mitochondria.
Journal of bioenergetics and biomembranes49(2)139-147(2017 Apr)
Ziamajidi N,Jamshidi S,Ehsani-Zonouz A

c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis.
Nature communications813732(2017 Jan)
Zhang J,Wang S,Jiang B,Huang L,Ji Z,Li X,Zhou H,Han A,Chen A,Wu Y,Ma H,Zhao W,Zhao Q,Xie C,Sun X,Zhou Y,Huang H,Suleman M,Lin F,Zhou L,Tian F,Jin M,Cai Y,Zhang N,Li Q

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Hk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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