P2rx6 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	P2rx6
Entrez Gene ID	25041
Full Name	purinergic receptor P2X 6
Synonyms	P2rxl1,P2x6,Rnap2x6
General protein information	Preferred Names purinergic receptor P2X 6 Names P2X purinoceptor 6 ATP receptor P2X6 receptor P2XM purinergic receptor P2X, ligand-gated ion channel, 6 purinergic receptor P2X-like 1 orphan receptor purinergic receptor P2X6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q23
Summary	acts as an ATP-gated ion channel [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017597883.1	XP_017453372.1	P2X purinoceptor 6 isoform X2
XM_017597882.1	XP_017453371.1	P2X purinoceptor 6 isoform X1
NM_012721.2	NP_036853.2	P2X purinoceptor 6

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	P2rx6	NP_035158.2
Gallus gallus (chicken)	LOC429367	XP_426924.4
Homo sapiens (human)	P2RX6	NP_005437.2
	P2RX6	XP_001084368.1
Bos taurus (cattle)	P2RX6	XP_005218354.1
Rattus norvegicus (Norway rat)	P2rx6	NP_036853.2
Canis lupus familiaris (dog)	P2RX6	XP_005636671.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential gene expression patterns and colocalization of ATP-gated P2X6/P2X4 ion channels during rat small intestine ontogeny.
Padilla K, Gonzalez-Mendoza D, Berumen LC, Escobar JE, Miledi R, Garc?a-Alcocer G
Gene expression patterns : GEP21(2)81-8(2016 Jul)

Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.
Liu Y, Tian X, Wu Y, Chen L, Yi CL, Li ZW, Zhang Y, Li CY
Biochemical and biophysical research communications458(3)596-600(2015 Mar)

Identification of P2X2/P2X4/P2X6 heterotrimeric receptors using atomic force microscopy (AFM) imaging.
Antonio LS, Stewart AP, Varanda WA, Edwardson JM
FEBS letters588(12)2125-8(2014 Jun)

Distribution of the purinegic receptors P2X(4) and P2X(6) during rat gut development.
Garc?a-Alcocer G, Padilla K, Rodr?guez A, Miledi R, Berumen LC
Neuroscience letters509(2)92-5(2012 Feb)

Spatiotemporal and anatomical analyses of P2X receptor-mediated neuronal and glial processing of sensory signals in the rat dorsal horn.
Aoyama R, Okada Y, Yokota S, Yasui Y, Fukuda K, Shinozaki Y, Yoshida H, Nakamura M, Chiba K, Yasui Y, Kato F, Toyama Y
Pain152(9)2085-97(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

P2X4 and P2X6 proteins were expressed in a different way during rat small intestine development, showing a higher colocalization coefficient at age P30 in both intestine regions. Those results suggest that purinergic receptors may play a role in intestinal maturation, which is associated with age and intestinal region.
Title: Differential gene expression patterns and colocalization of ATP-gated P2X6/P2X4 ion channels during rat small intestine ontogeny.

The study explores the association of the phenotype of ATP-activated current with the genotype of P2X1-6 subunits in neurons innervating tooth-pulp.
Title: Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.

P2X(4) and P2X(6) purinoreceptors are present in the gut from birth and that they are differentially distributed among regions
Title: Distribution of the purinegic receptors P2X(4) and P2X(6) during rat gut development.

The P2X6 receptor subunit is widely expressed in neuropils in the whole gray matter except for the dorsal superficial layer of the dorsal horn.
Title: Spatiotemporal and anatomical analyses of P2X receptor-mediated neuronal and glial processing of sensory signals in the rat dorsal horn.

Results suggest a direct relationship between purinergic P2X2/6 receptor expression and induction of neuronal differentiation in mitogen-free cultured rat neurospheres.
Title: Directed differentiation of neural progenitors into neurons is accompanied by altered expression of P2X purinergic receptors.

The following P2rx6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the P2rx6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00753	NM_012721.2 Latest version!	Rattus norvegicus purinergic receptor P2X 6 (P2rx6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa57645	XM_017597882.1 Latest version!	Rattus norvegicus purinergic receptor P2X 6 (P2rx6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa57646	XM_017597883.1 Latest version!	Rattus norvegicus purinergic receptor P2X 6 (P2rx6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

P2rx6 ( NM_012721.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036853.2 Rattus norvegicus purinergic receptor P2X 6 (P2rx6), mRNA.

Price & Availability↑

CloneID	ORa00753
Clone ID Related Accession (Same CDS sequence)	NM_012721.2
Accession Version	NM_012721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	P2X purinoceptor 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from X97376.1, CO554543.1 and X92070.1. On Apr 21, 2009 this sequence version replaced NM_012721.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X92070.1, X97376.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGTTGC AGCCAGCGGG AACTGGGAGC ATGGCTTCTG CAGTCGCAGC AGCACTAGTG 
AGCTGGGGGT TTCTGGATTA CAAGACAGAG AAATATGTGA TGACCAGGAA CTGCTGGGTG 
GGCATCTCCC AGAGGCTGCT GCAGCTCGGA GTGGTAGTCT ACGTGATTGG GTGGGCCCTC 
TTGGCCAAAA AAGGCTACCA GGAATGGGAC ATGGACCCCC AGATTTCTGT CATCACCAAA 
CTCAAGGGGG TTTCTGTAAC CCAGGTTAAG GAACTGGAGA AACGGCTGTG GGATGTGGCT 
GACTTCGTGA GGCCATCACA GGGAGAGAAT GTGTTCTTCC TGGTAACCAA CTTCCTTGTG 
ACACCAGCTC AAGTCCAGGG CAGATGCCCA GAGCATCCTT CTGTTCCTCT GGCTAACTGC 
TGGGCTGATG AGGACTGTCC TGAAGGGGAG ATGGGGACAT ACAGCCACGG CATAAAGACT 
GGTCAGTGTG TGGCGTTCAA CGGAACCCAC AGGACCTGTG AGATCTGGAG CTGGTGCCCA 
GTGGAGAGTA GTGCTGTGCC CAGGAAACCC CTGCTAGCTC AGGCCAAGAA CTTCACGCTC 
TTCATCAAAA ATACTGTAAC CTTCAACAAG TTCAACTTCT CCAGAACCAA TGCCTTAGAT 
ACCTGGGACA ACACCTATTT CAAGTACTGT CTCTACGATT CACTCTCCAG TCCGTACTGC 
CCAGTGTTCC GCATCGGGGA CCTTGTGGCT ATGACTGGAG GGGACTTTGA GGACCTTGCA 
TTGCTGGGTG GTGCTGTGGG CATCAACATC CACTGGGATT GCAACCTGGA CACGAAAGGC 
TCTGACTGCT CTCCCCAGTA CTCCTTCCAG CTGCAGGAGA GGGGGTACAA TTTCAGGACA 
GCTAATTACT GGTGGGCAGC CTCAGGCGTG GAGTCCCGGA GCCTGCTCAA ACTCTATGGG 
ATCCGCTTTG ACATTCTTGT CACTGGGCAG GCAGGAAAGT TTGCACTCAT CCCTACGGCC 
ATCACAGTGG GCACAGGAGC AGCTTGGCTG GGCATGGTCA CCTTCCTCTG TGATCTGCTG 
CTACTGTACG TGGATAGAGA GGCCGGTTTC TACTGGAGGA CCAAGTATGA AGAGGCAAGA 
GCCCCAAAGG CAACTACCAA CAGTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036853.2
CDS	103..1272
Translation	MQLQPAGTGSMASAVAAALVSWGFLDYKTEKYVMTRNCWVGISQRLLQLGVVVYVIGWALLAKKGYQEWDMDPQISVITKLKGVSVTQVKELEKRLWDVADFVRPSQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWADEDCPEGEMGTYSHGIKTGQCVAFNGTHRTCEIWSWCPVESSAVPRKPLLAQAKNFTLFIKNTVTFNKFNFSRTNALDTWDNTYFKYCLYDSLSSPYCPVFRIGDLVAMTGGDFEDLALLGGAVGINIHWDCNLDTKGSDCSPQYSFQLQERGYNFRTANYWWAASGVESRSLLKLYGIRFDILVTGQAGKFALIPTAITVGTGAAWLGMVTFLCDLLLLYVDREAGFYWRTKYEEARAPKATTNSA

Target ORF information:

RefSeq Version	NM_012721.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus purinergic receptor P2X 6 (P2rx6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012721.2

ORF Insert Sequence:

ATGCAGTTGC AGCCAGCGGG AACTGGGAGC ATGGCTTCTG CAGTCGCAGC AGCACTAGTG 
AGCTGGGGGT TTCTGGATTA CAAGACAGAG AAATATGTGA TGACCAGGAA CTGCTGGGTG 
GGCATCTCCC AGAGGCTGCT GCAGCTCGGA GTGGTAGTCT ACGTGATTGG GTGGGCCCTC 
TTGGCCAAAA AAGGCTACCA GGAATGGGAC ATGGACCCCC AGATTTCTGT CATCACCAAA 
CTCAAGGGGG TTTCTGTAAC CCAGGTTAAG GAACTGGAGA AACGGCTGTG GGATGTGGCT 
GACTTCGTGA GGCCATCACA GGGAGAGAAT GTGTTCTTCC TGGTAACCAA CTTCCTTGTG 
ACACCAGCTC AAGTCCAGGG CAGATGCCCA GAGCATCCTT CTGTTCCTCT GGCTAACTGC 
TGGGCTGATG AGGACTGTCC TGAAGGGGAG ATGGGGACAT ACAGCCACGG CATAAAGACT 
GGTCAGTGTG TGGCGTTCAA CGGAACCCAC AGGACCTGTG AGATCTGGAG CTGGTGCCCA 
GTGGAGAGTA GTGCTGTGCC CAGGAAACCC CTGCTAGCTC AGGCCAAGAA CTTCACGCTC 
TTCATCAAAA ATACTGTAAC CTTCAACAAG TTCAACTTCT CCAGAACCAA TGCCTTAGAT 
ACCTGGGACA ACACCTATTT CAAGTACTGT CTCTACGATT CACTCTCCAG TCCGTACTGC 
CCAGTGTTCC GCATCGGGGA CCTTGTGGCT ATGACTGGAG GGGACTTTGA GGACCTTGCA 
TTGCTGGGTG GTGCTGTGGG CATCAACATC CACTGGGATT GCAACCTGGA CACGAAAGGC 
TCTGACTGCT CTCCCCAGTA CTCCTTCCAG CTGCAGGAGA GGGGGTACAA TTTCAGGACA 
GCTAATTACT GGTGGGCAGC CTCAGGCGTG GAGTCCCGGA GCCTGCTCAA ACTCTATGGG 
ATCCGCTTTG ACATTCTTGT CACTGGGCAG GCAGGAAAGT TTGCACTCAT CCCTACGGCC 
ATCACAGTGG GCACAGGAGC AGCTTGGCTG GGCATGGTCA CCTTCCTCTG TGATCTGCTG 
CTACTGTACG TGGATAGAGA GGCCGGTTTC TACTGGAGGA CCAAGTATGA AGAGGCAAGA 
GCCCCAAAGG CAACTACCAA CAGTGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P2rx6 ( XM_017597882.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453371.1 Rattus norvegicus purinergic receptor P2X 6 (P2rx6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57645
Clone ID Related Accession (Same CDS sequence)	XM_017597882.1
Accession Version	XM_017597882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	P2X purinoceptor 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC GCCAGCAGGG ATATCTCAAA CCCAACTTGT ACTCTTCGGA ATCTGCAAGT 
TTGGGTTTAG ATAACCTCTT CTCTGGGCTT CTGTTACCCT GTATCGACTG CCCACCCTAT 
AAGAAAGCAG AGGGAGGGTG GGCCCTCTTG GCCAAAAAAG GCTACCAGGA ATGGGACATG 
GACCCCCAGA TTTCTGTCAT CACCAAACTC AAGGGGGTTT CTGTAACCCA GGTTAAGGAA 
CTGGAGAAAC GGCTGTGGGA TGTGGCTGAC TTCGTGAGGC CATCACAGGG AGAGAATGTG 
TTCTTCCTGG TAACCAACTT CCTTGTGACA CCAGCTCAAG TCCAGGGCAG ATGCCCAGAG 
CATCCTTCTG TTCCTCTGGC TAACTGCTGG GCTGATGAGG ACTGTCCTGA AGGGGAGATG 
GGGACATACA GCCACGGCAT AAAGACTGGT CAGTGTGTGG CGTTCAACGG AACCCACAGG 
ACCTGTGAGA TCTGGAGCTG GTGCCCAGTG GAGAGTAGTG CTGTGCCCAG GAAACCCCTG 
CTAGCTCAGG CCAAGAACTT CACGCTCTTC ATCAAAAATA CTGTAACCTT CAACAAGTTC 
AACTTCTCCA GAACCAATGC CTTAGATACC TGGGACAACA CCTATTTCAA GTACTGTCTC 
TACGATTCAC TCTCCAGTCC GTACTGCCCA GTGTTCCGCA TCGGGGACCT TGTGGCTATG 
ACTGGAGGGG ACTTTGAGGA CCTTGCATTG CTGGGTGGTG CTGTGGGCAT CAACATCCAC 
TGGGATTGCA ACCTGGACAC GAAAGGCTCT GACTGCTCTC CCCAGTACTC CTTCCAGCTG 
CAGGAGAGGG GGTACAATTT CAGGACAGCT AATTACTGGT GGGCAGCCTC AGGCGTGGAG 
TCCCGGAGCC TGCTCAAACT CTATGGGATC CGCTTTGACA TTCTTGTCAC TGGGCAGGCA 
GGAAAGTTTG CACTCATCCC TACGGCCATC ACAGTGGGCA CAGGAGCAGC TTGGCTGGGC 
ATGGTCACCT TCCTCTGTGA TCTGCTGCTA CTGTACGTGG ATAGAGAGGC CGGTTTCTAC 
TGGAGGACCA AGTATGAAGA GGCAAGAGCC CCAAAGGCAA CTACCAACAG TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453371.1
CDS	13..1149
Translation	MLLRQQGYLKPNLYSSESASLGLDNLFSGLLLPCIDCPPYKKAEGGWALLAKKGYQEWDMDPQISVITKLKGVSVTQVKELEKRLWDVADFVRPSQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWADEDCPEGEMGTYSHGIKTGQCVAFNGTHRTCEIWSWCPVESSAVPRKPLLAQAKNFTLFIKNTVTFNKFNFSRTNALDTWDNTYFKYCLYDSLSSPYCPVFRIGDLVAMTGGDFEDLALLGGAVGINIHWDCNLDTKGSDCSPQYSFQLQERGYNFRTANYWWAASGVESRSLLKLYGIRFDILVTGQAGKFALIPTAITVGTGAAWLGMVTFLCDLLLLYVDREAGFYWRTKYEEARAPKATTNSA

Target ORF information:

RefSeq Version	XM_017597882.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus purinergic receptor P2X 6 (P2rx6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597882.1

ORF Insert Sequence:

ATGCTGCTGC GCCAGCAGGG ATATCTCAAA CCCAACTTGT ACTCTTCGGA ATCTGCAAGT 
TTGGGTTTAG ATAACCTCTT CTCTGGGCTT CTGTTACCCT GTATCGACTG CCCACCCTAT 
AAGAAAGCAG AGGGAGGGTG GGCCCTCTTG GCCAAAAAAG GCTACCAGGA ATGGGACATG 
GACCCCCAGA TTTCTGTCAT CACCAAACTC AAGGGGGTTT CTGTAACCCA GGTTAAGGAA 
CTGGAGAAAC GGCTGTGGGA TGTGGCTGAC TTCGTGAGGC CATCACAGGG AGAGAATGTG 
TTCTTCCTGG TAACCAACTT CCTTGTGACA CCAGCTCAAG TCCAGGGCAG ATGCCCAGAG 
CATCCTTCTG TTCCTCTGGC TAACTGCTGG GCTGATGAGG ACTGTCCTGA AGGGGAGATG 
GGGACATACA GCCACGGCAT AAAGACTGGT CAGTGTGTGG CGTTCAACGG AACCCACAGG 
ACCTGTGAGA TCTGGAGCTG GTGCCCAGTG GAGAGTAGTG CTGTGCCCAG GAAACCCCTG 
CTAGCTCAGG CCAAGAACTT CACGCTCTTC ATCAAAAATA CTGTAACCTT CAACAAGTTC 
AACTTCTCCA GAACCAATGC CTTAGATACC TGGGACAACA CCTATTTCAA GTACTGTCTC 
TACGATTCAC TCTCCAGTCC GTACTGCCCA GTGTTCCGCA TCGGGGACCT TGTGGCTATG 
ACTGGAGGGG ACTTTGAGGA CCTTGCATTG CTGGGTGGTG CTGTGGGCAT CAACATCCAC 
TGGGATTGCA ACCTGGACAC GAAAGGCTCT GACTGCTCTC CCCAGTACTC CTTCCAGCTG 
CAGGAGAGGG GGTACAATTT CAGGACAGCT AATTACTGGT GGGCAGCCTC AGGCGTGGAG 
TCCCGGAGCC TGCTCAAACT CTATGGGATC CGCTTTGACA TTCTTGTCAC TGGGCAGGCA 
GGAAAGTTTG CACTCATCCC TACGGCCATC ACAGTGGGCA CAGGAGCAGC TTGGCTGGGC 
ATGGTCACCT TCCTCTGTGA TCTGCTGCTA CTGTACGTGG ATAGAGAGGC CGGTTTCTAC 
TGGAGGACCA AGTATGAAGA GGCAAGAGCC CCAAAGGCAA CTACCAACAG TGCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

P2rx6 ( XM_017597883.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453372.1 Rattus norvegicus purinergic receptor P2X 6 (P2rx6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57646
Clone ID Related Accession (Same CDS sequence)	XM_017597883.1
Accession Version	XM_017597883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	P2X purinoceptor 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTGC AGCCAGCGGG AACTGGGAGC ATGGCTTCTG CAGTCGCAGC AGCACTAGTT 
AAGGAACTGG AGAAACGGCT GTGGGATGTG GCTGACTTCG TGAGGCCATC ACAGGGAGAG 
AATGTGTTCT TCCTGGTAAC CAACTTCCTT GTGACACCAG CTCAAGTCCA GGGCAGATGC 
CCAGAGCATC CTTCTGTTCC TCTGGCTAAC TGCTGGGCTG ATGAGGACTG TCCTGAAGGG 
GAGATGGGGA CATACAGCCA CGGCATAAAG ACTGGTCAGT GTGTGGCGTT CAACGGAACC 
CACAGGACCT GTGAGATCTG GAGCTGGTGC CCAGTGGAGA GTAGTGCTGT GCCCAGGAAA 
CCCCTGCTAG CTCAGGCCAA GAACTTCACG CTCTTCATCA AAAATACTGT AACCTTCAAC 
AAGTTCAACT TCTCCAGAAC CAATGCCTTA GATACCTGGG ACAACACCTA TTTCAAGTAC 
TGTCTCTACG ATTCACTCTC CAGTCCGTAC TGCCCAGTGT TCCGCATCGG GGACCTTGTG 
GCTATGACTG GAGGGGACTT TGAGGACCTT GCATTGCTGG GTGGTGCTGT GGGCATCAAC 
ATCCACTGGG ATTGCAACCT GGACACGAAA GGCTCTGACT GCTCTCCCCA GTACTCCTTC 
CAGCTGCAGG AGAGGGGGTA CAATTTCAGG ACAGCTAATT ACTGGTGGGC AGCCTCAGGC 
GTGGAGTCCC GGAGCCTGCT CAAACTCTAT GGGATCCGCT TTGACATTCT TGTCACTGGG 
CAGGCAGGAA AGTTTGCACT CATCCCTACG GCCATCACAG TGGGCACAGG AGCAGCTTGG 
CTGGGCATGG TCACCTTCCT CTGTGATCTG CTGCTACTGT ACGTGGATAG AGAGGCCGGT 
TTCTACTGGA GGACCAAGTA TGAAGAGGCA AGAGCCCCAA AGGCAACTAC CAACAGTGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453372.1
CDS	196..1158
Translation	MQLQPAGTGSMASAVAAALVKELEKRLWDVADFVRPSQGENVFFLVTNFLVTPAQVQGRCPEHPSVPLANCWADEDCPEGEMGTYSHGIKTGQCVAFNGTHRTCEIWSWCPVESSAVPRKPLLAQAKNFTLFIKNTVTFNKFNFSRTNALDTWDNTYFKYCLYDSLSSPYCPVFRIGDLVAMTGGDFEDLALLGGAVGINIHWDCNLDTKGSDCSPQYSFQLQERGYNFRTANYWWAASGVESRSLLKLYGIRFDILVTGQAGKFALIPTAITVGTGAAWLGMVTFLCDLLLLYVDREAGFYWRTKYEEARAPKATTNSA

Target ORF information:

RefSeq Version	XM_017597883.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus purinergic receptor P2X 6 (P2rx6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597883.1

ORF Insert Sequence:

ATGCAGTTGC AGCCAGCGGG AACTGGGAGC ATGGCTTCTG CAGTCGCAGC AGCACTAGTT 
AAGGAACTGG AGAAACGGCT GTGGGATGTG GCTGACTTCG TGAGGCCATC ACAGGGAGAG 
AATGTGTTCT TCCTGGTAAC CAACTTCCTT GTGACACCAG CTCAAGTCCA GGGCAGATGC 
CCAGAGCATC CTTCTGTTCC TCTGGCTAAC TGCTGGGCTG ATGAGGACTG TCCTGAAGGG 
GAGATGGGGA CATACAGCCA CGGCATAAAG ACTGGTCAGT GTGTGGCGTT CAACGGAACC 
CACAGGACCT GTGAGATCTG GAGCTGGTGC CCAGTGGAGA GTAGTGCTGT GCCCAGGAAA 
CCCCTGCTAG CTCAGGCCAA GAACTTCACG CTCTTCATCA AAAATACTGT AACCTTCAAC 
AAGTTCAACT TCTCCAGAAC CAATGCCTTA GATACCTGGG ACAACACCTA TTTCAAGTAC 
TGTCTCTACG ATTCACTCTC CAGTCCGTAC TGCCCAGTGT TCCGCATCGG GGACCTTGTG 
GCTATGACTG GAGGGGACTT TGAGGACCTT GCATTGCTGG GTGGTGCTGT GGGCATCAAC 
ATCCACTGGG ATTGCAACCT GGACACGAAA GGCTCTGACT GCTCTCCCCA GTACTCCTTC 
CAGCTGCAGG AGAGGGGGTA CAATTTCAGG ACAGCTAATT ACTGGTGGGC AGCCTCAGGC 
GTGGAGTCCC GGAGCCTGCT CAAACTCTAT GGGATCCGCT TTGACATTCT TGTCACTGGG 
CAGGCAGGAA AGTTTGCACT CATCCCTACG GCCATCACAG TGGGCACAGG AGCAGCTTGG 
CTGGGCATGG TCACCTTCCT CTGTGATCTG CTGCTACTGT ACGTGGATAG AGAGGCCGGT 
TTCTACTGGA GGACCAAGTA TGAAGAGGCA AGAGCCCCAA AGGCAACTAC CAACAGTGCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential gene expression patterns and colocalization of ATP-gated P2X6/P2X4 ion channels during rat small intestine ontogeny.
Gene expression patterns : GEP21(2)81-8(2016 Jul)
Padilla K,Gonzalez-Mendoza D,Berumen LC,Escobar JE,Miledi R,Garc?a-Alcocer G

Phenotypes of ATP-activated current associated with their genotypes of P2X1-6 subunits in neurons innervating tooth-pulp.
Biochemical and biophysical research communications458(3)596-600(2015 Mar)
Liu Y,Tian X,Wu Y,Chen L,Yi CL,Li ZW,Zhang Y,Li CY

Identification of P2X2/P2X4/P2X6 heterotrimeric receptors using atomic force microscopy (AFM) imaging.
FEBS letters588(12)2125-8(2014 Jun)
Antonio LS,Stewart AP,Varanda WA,Edwardson JM

Distribution of the purinegic receptors P2X(4) and P2X(6) during rat gut development.
Neuroscience letters509(2)92-5(2012 Feb)
Garc?a-Alcocer G,Padilla K,Rodr?guez A,Miledi R,Berumen LC

Spatiotemporal and anatomical analyses of P2X receptor-mediated neuronal and glial processing of sensory signals in the rat dorsal horn.
Pain152(9)2085-97(2011 Sep)
Aoyama R,Okada Y,Yokota S,Yasui Y,Fukuda K,Shinozaki Y,Yoshida H,Nakamura M,Chiba K,Yasui Y,Kato F,Toyama Y

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P2rx6 cDNA ORF clone, Rattus norvegicus(Norway rat)

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