Gcgr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gcgr
Entrez Gene ID	24953
Full Name	glucagon receptor
General protein information	Preferred Names glucagon receptor Names glucagon receptor GL-R glucagon receptor perhaps same as Niddm3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3
Summary	G-protein coupled receptor that binds glucagon and activates adenylate cyclase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172091.2	NP_742088.1	glucagon receptor precursor
XM_006247876.3	XP_006247938.1	glucagon receptor isoform X2
XM_006247877.2	XP_006247939.1	glucagon receptor isoform X4
XM_008768468.2	XP_008766690.1	glucagon receptor isoform X1
XM_008768469.2	XP_008766691.1	glucagon receptor isoform X3
NM_172092.2	NP_742089.1	glucagon receptor precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Gcgr	NP_742089.1
Danio rerio (zebrafish)	gcgrb	XP_693606.3
Canis lupus familiaris (dog)	GCGR	XP_005624057.1
Bos taurus (cattle)	GCGR	NP_001179343.1
Mus musculus (house mouse)	Gcgr	NP_032127.2
Xenopus tropicalis (tropical clawed frog)	gcgr	XP_004916426.1
Homo sapiens (human)	GCGR	NP_000151.1
Pan troglodytes (chimpanzee)	GCGR	XP_523746.2
	GCGR	XP_001111894.1
Gallus gallus (chicken)	GIPR	NP_001094505.1
Danio rerio (zebrafish)	LOC562973	XP_691434.5

Gene Ontology
Bibliography

Related articles in PubMed

Structure of the full-length glucagon class B G-protein-coupled receptor.
Zhang H, Qiao A, Yang D, Yang L, Dai A, de Graaf C, Reedtz-Runge S, Dharmarajan V, Zhang H, Han GW, Grant TD, Sierra RG, Weierstall U, Nelson G, Liu W, Wu Y, Ma L, Cai X, Lin G, Wu X, Geng Z, Dong Y, Song G, Griffin PR, Lau J, Cherezov V, Yang H, Hanson MA, Stevens RC, Zhao Q, Jiang H, Wang MW, Wu B
Nature546(7657)259-264(2017 06)

Vasodilator Effect of Glucagon: Receptorial Crosstalk Among Glucagon, GLP-1, and Receptor for Glucagon and GLP-1.
S?lley E, Kun S, Szij?rt? IA, Kert?sz M, Wittmann I, Moln?r GA
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme48(7)476-83(2016 Jul)

Liver glucagon receptors (GluR): effect of exercise and fasting on binding characteristics, GluR-mRNA, and GluR protein content in rats.
Melan?on A, Gagnon V, Milot M, Charest ?, Foucher D, P?ronnet F, Unson O'Brien CG, Asselin E, Lavoie C
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(10)716-21(2013 Sep)

Glucagon receptor recycling: role of carboxyl terminus, beta-arrestins, and cytoskeleton.
Krilov L, Nguyen A, Miyazaki T, Unson CG, Bouscarel B
American journal of physiology. Cell physiology295(5)C1230-7(2008 Nov)

The glucagon receptor is required for the adaptive metabolic response to fasting.
Longuet C, Sinclair EM, Maida A, Baggio LL, Maziarz M, Charron MJ, Drucker DJ
Cell metabolism8(5)359-71(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The vasodilatation due to glucagon evokes via the glucagon-receptor, but also via the receptor for GLP-1, and it is endothelium-independent.
Title: Vasodilator Effect of Glucagon: Receptorial Crosstalk Among Glucagon, GLP-1, and Receptor for Glucagon and GLP-1.

Significant higher GluR density (mRNA and proteins) following exercise and fasting than in the control group.
Title: Liver glucagon receptors (GluR): effect of exercise and fasting on binding characteristics, GluR-mRNA, and GluR protein content in rats.

The actin cytoskeleton, beta-arrestin1, beta-arrestin2, and the glucagon receptor's carboxyl terminus are involved in its recycling.
Title: Glucagon receptor recycling: role of carboxyl terminus, beta-arrestins, and cytoskeleton.

These results suggest that glucagon stimulates acute release of both forms of ghrelin and thereafter upregulates synthesis and release of desacyl-ghrelin in the rat stomach.
Title: Glucagon receptor expression and glucagon stimulation of ghrelin secretion in rat stomach.

High fat diet induced obesity is associated with a desensitization and destruction of the hepatic GR
Title: High-fat diet-induced hepatic steatosis reduces glucagon receptor content in rat hepatocytes: potential interaction with acute exercise.

The following Gcgr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gcgr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12671	NM_172092.2 Latest version!	Rattus norvegicus glucagon receptor (Gcgr), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa12671	NM_172091.2 Latest version!	Rattus norvegicus glucagon receptor (Gcgr), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa12671	XM_006247876.3 Latest version!	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa34025	XM_006247877.2 Latest version!	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42995	XM_008768468.2 Latest version!	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42996	XM_008768469.2 Latest version!	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gcgr ( NM_172091.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742088.1 Rattus norvegicus glucagon receptor (Gcgr), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12671
Clone ID Related Accession (Same CDS sequence)	NM_172091.2 , XM_006247876.3 , NM_172092.2
Accession Version	NM_172091.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	glucagon receptor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB735411.1, BC081700.1 and CV117246.1. On Sep 6, 2012 this sequence version replaced NM_172091.1. Transcript Variant: This variant (2) contains an alternate 5' terminal non-coding exon, therefore, has a different 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L04796.1, X68692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGCCAAAGG CACCCTCTGC CCAGGTAATG GACTTTTTGT TTGAGAAGTG GAAGCTCTAT 
AGTGACCAGT GCCACCACAA CCTAAGCCTG CTGCCCCCAC CTACTGAGCT GGTCTGCAAC 
AGAACTTTCG ACAAGTACTC CTGCTGGCCT GACACCCCTC CCAACACCAC TGCCAACATT 
TCCTGCCCCT GGTACCTACC TTGGTACCAC AAAGTGCAGC ACCGCCTAGT GTTCAAGAGG 
TGTGGGCCTG ATGGGCAGTG GGTTCGAGGG CCACGGGGGC AGTCATGGCG CGACGCCTCC 
CAATGTCAGA TGGATGATGA CGAGATCGAG GTCCAGAAGG GGGTAGCCAA GATGTATAGC 
AGCTACCAGG TGATGTACAC TGTGGGCTAC AGTCTGTCCC TGGGGGCCTT GCTCCTGGCG 
CTGGTCATCC TGCTGGGCCT CAGGAAGCTG CACTGCACCC GGAACTACAT CCACGGGAAC 
CTGTTCGCGT CCTTCGTGCT CAAGGCTGGC TCTGTGCTGG TCATTGATTG GCTGCTCAAG 
ACACGCTATA GCCAGAAGAT TGGAGATGAC CTCAGTGTGA GCGTCTGGCT CAGTGATGGG 
GCGGTGGCTG GCTGCAGAGT GGCCACAGTG ATCATGCAGT ACGGCATCAT AGCCAACTAC 
TGCTGGTTGC TGGTGGAGGG TGTGTACCTG TACAGCCTGC TGAGCATCAC CACCTTCTCG 
GAGAAGAGCT TCTTCTCCCT CTATCTGTGC ATCGGCTGGG GATCTCCCCT GCTGTTTGTC 
ATCCCCTGGG TGGTGGTCAA GTGTCTGTTT GAGAATGTCC AGTGCTGGAC CAGCAATGAC 
AATATGGGAT TCTGGTGGAT CCTGCGTATC CCTGTACTCC TGGCCATACT GATCAATTTT 
TTCATCTTTG TCCGCATCAT TCATCTTCTT GTGGCCAAGC TGCGTGCCCA TCAGATGCAC 
TATGCTGATT ACAAGTTCCG GCTAGCCAGG TCCACGCTGA CCCTCATTCC TCTGCTGGGA 
GTCCACGAAG TGGTCTTTGC CTTTGTGACT GATGAGCATG CCCAGGGCAC CCTGCGCTCC 
ACCAAGCTCT TTTTTGACCT GTTCTTCAGC TCCTTCCAGG GTCTGCTGGT GGCTGTTCTC 
TACTGTTTCC TCAACAAGGA GGTGCAGGCA GAGCTACTGC GGCGTTGGAG GCGATGGCAA 
GAAGGCAAAG CTCTTCAGGA GGAAAGGATG GCCAGCAGCC ATGGCAGCCA CATGGCCCCA 
GCAGGGACTT GTCATGGTGA TCCCTGTGAG AAACTTCAGC TTATGAGTGC AGGCAGCAGC 
AGTGGGACTG GCTGTGAGCC CTCTGCGAAG ACCTCATTGG CCAGTAGTCT CCCAAGGCTG 
GCTGACAGCC CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742088.1
CDS	135..1592
Translation	MLLTQLHCPYLLLLLVVLSCLPKAPSAQVMDFLFEKWKLYSDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPPNTTANISCPWYLPWYHKVQHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAKMYSSYQVMYTVGYSLSLGALLLALVILLGLRKLHCTRNYIHGNLFASFVLKAGSVLVIDWLLKTRYSQKIGDDLSVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCIGWGSPLLFVIPWVVVKCLFENVQCWTSNDNMGFWWILRIPVLLAILINFFIFVRIIHLLVAKLRAHQMHYADYKFRLARSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSTKLFFDLFFSSFQGLLVAVLYCFLNKEVQAELLRRWRRWQEGKALQEERMASSHGSHMAPAGTCHGDPCEKLQLMSAGSSSGTGCEPSAKTSLASSLPRLADSPT

Target ORF information:

RefSeq Version	NM_172091.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucagon receptor (Gcgr), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172091.2

ORF Insert Sequence:

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGCCAAAGG CACCCTCTGC CCAGGTAATG GACTTTTTGT TTGAGAAGTG GAAGCTCTAT 
AGTGACCAGT GCCACCACAA CCTAAGCCTG CTGCCCCCAC CTACTGAGCT GGTCTGCAAC 
AGAACTTTCG ACAAGTACTC CTGCTGGCCT GACACCCCTC CCAACACCAC TGCCAACATT 
TCCTGCCCCT GGTACCTACC TTGGTACCAC AAAGTGCAGC ACCGCCTAGT GTTCAAGAGG 
TGTGGGCCTG ATGGGCAGTG GGTTCGAGGG CCACGGGGGC AGTCATGGCG CGACGCCTCC 
CAATGTCAGA TGGATGATGA CGAGATCGAG GTCCAGAAGG GGGTAGCCAA GATGTATAGC 
AGCTACCAGG TGATGTACAC TGTGGGCTAC AGTCTGTCCC TGGGGGCCTT GCTCCTGGCG 
CTGGTCATCC TGCTGGGCCT CAGGAAGCTG CACTGCACCC GGAACTACAT CCACGGGAAC 
CTGTTCGCGT CCTTCGTGCT CAAGGCTGGC TCTGTGCTGG TCATTGATTG GCTGCTCAAG 
ACACGCTATA GCCAGAAGAT TGGAGATGAC CTCAGTGTGA GCGTCTGGCT CAGTGATGGG 
GCGGTGGCTG GCTGCAGAGT GGCCACAGTG ATCATGCAGT ACGGCATCAT AGCCAACTAC 
TGCTGGTTGC TGGTGGAGGG TGTGTACCTG TACAGCCTGC TGAGCATCAC CACCTTCTCG 
GAGAAGAGCT TCTTCTCCCT CTATCTGTGC ATCGGCTGGG GATCTCCCCT GCTGTTTGTC 
ATCCCCTGGG TGGTGGTCAA GTGTCTGTTT GAGAATGTCC AGTGCTGGAC CAGCAATGAC 
AATATGGGAT TCTGGTGGAT CCTGCGTATC CCTGTACTCC TGGCCATACT GATCAATTTT 
TTCATCTTTG TCCGCATCAT TCATCTTCTT GTGGCCAAGC TGCGTGCCCA TCAGATGCAC 
TATGCTGATT ACAAGTTCCG GCTAGCCAGG TCCACGCTGA CCCTCATTCC TCTGCTGGGA 
GTCCACGAAG TGGTCTTTGC CTTTGTGACT GATGAGCATG CCCAGGGCAC CCTGCGCTCC 
ACCAAGCTCT TTTTTGACCT GTTCTTCAGC TCCTTCCAGG GTCTGCTGGT GGCTGTTCTC 
TACTGTTTCC TCAACAAGGA GGTGCAGGCA GAGCTACTGC GGCGTTGGAG GCGATGGCAA 
GAAGGCAAAG CTCTTCAGGA GGAAAGGATG GCCAGCAGCC ATGGCAGCCA CATGGCCCCA 
GCAGGGACTT GTCATGGTGA TCCCTGTGAG AAACTTCAGC TTATGAGTGC AGGCAGCAGC 
AGTGGGACTG GCTGTGAGCC CTCTGCGAAG ACCTCATTGG CCAGTAGTCT CCCAAGGCTG 
GCTGACAGCC CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcgr ( NM_172092.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742089.1 Rattus norvegicus glucagon receptor (Gcgr), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12671
Clone ID Related Accession (Same CDS sequence)	NM_172091.2 , XM_006247876.3 , NM_172092.2
Accession Version	NM_172092.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	glucagon receptor precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC131537.5, BC081700.1 and CV117246.1. On Sep 6, 2012 this sequence version replaced NM_172092.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M96674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689598 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGCCAAAGG CACCCTCTGC CCAGGTAATG GACTTTTTGT TTGAGAAGTG GAAGCTCTAT 
AGTGACCAGT GCCACCACAA CCTAAGCCTG CTGCCCCCAC CTACTGAGCT GGTCTGCAAC 
AGAACTTTCG ACAAGTACTC CTGCTGGCCT GACACCCCTC CCAACACCAC TGCCAACATT 
TCCTGCCCCT GGTACCTACC TTGGTACCAC AAAGTGCAGC ACCGCCTAGT GTTCAAGAGG 
TGTGGGCCTG ATGGGCAGTG GGTTCGAGGG CCACGGGGGC AGTCATGGCG CGACGCCTCC 
CAATGTCAGA TGGATGATGA CGAGATCGAG GTCCAGAAGG GGGTAGCCAA GATGTATAGC 
AGCTACCAGG TGATGTACAC TGTGGGCTAC AGTCTGTCCC TGGGGGCCTT GCTCCTGGCG 
CTGGTCATCC TGCTGGGCCT CAGGAAGCTG CACTGCACCC GGAACTACAT CCACGGGAAC 
CTGTTCGCGT CCTTCGTGCT CAAGGCTGGC TCTGTGCTGG TCATTGATTG GCTGCTCAAG 
ACACGCTATA GCCAGAAGAT TGGAGATGAC CTCAGTGTGA GCGTCTGGCT CAGTGATGGG 
GCGGTGGCTG GCTGCAGAGT GGCCACAGTG ATCATGCAGT ACGGCATCAT AGCCAACTAC 
TGCTGGTTGC TGGTGGAGGG TGTGTACCTG TACAGCCTGC TGAGCATCAC CACCTTCTCG 
GAGAAGAGCT TCTTCTCCCT CTATCTGTGC ATCGGCTGGG GATCTCCCCT GCTGTTTGTC 
ATCCCCTGGG TGGTGGTCAA GTGTCTGTTT GAGAATGTCC AGTGCTGGAC CAGCAATGAC 
AATATGGGAT TCTGGTGGAT CCTGCGTATC CCTGTACTCC TGGCCATACT GATCAATTTT 
TTCATCTTTG TCCGCATCAT TCATCTTCTT GTGGCCAAGC TGCGTGCCCA TCAGATGCAC 
TATGCTGATT ACAAGTTCCG GCTAGCCAGG TCCACGCTGA CCCTCATTCC TCTGCTGGGA 
GTCCACGAAG TGGTCTTTGC CTTTGTGACT GATGAGCATG CCCAGGGCAC CCTGCGCTCC 
ACCAAGCTCT TTTTTGACCT GTTCTTCAGC TCCTTCCAGG GTCTGCTGGT GGCTGTTCTC 
TACTGTTTCC TCAACAAGGA GGTGCAGGCA GAGCTACTGC GGCGTTGGAG GCGATGGCAA 
GAAGGCAAAG CTCTTCAGGA GGAAAGGATG GCCAGCAGCC ATGGCAGCCA CATGGCCCCA 
GCAGGGACTT GTCATGGTGA TCCCTGTGAG AAACTTCAGC TTATGAGTGC AGGCAGCAGC 
AGTGGGACTG GCTGTGAGCC CTCTGCGAAG ACCTCATTGG CCAGTAGTCT CCCAAGGCTG 
GCTGACAGCC CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742089.1
CDS	143..1600
Translation	MLLTQLHCPYLLLLLVVLSCLPKAPSAQVMDFLFEKWKLYSDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPPNTTANISCPWYLPWYHKVQHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAKMYSSYQVMYTVGYSLSLGALLLALVILLGLRKLHCTRNYIHGNLFASFVLKAGSVLVIDWLLKTRYSQKIGDDLSVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCIGWGSPLLFVIPWVVVKCLFENVQCWTSNDNMGFWWILRIPVLLAILINFFIFVRIIHLLVAKLRAHQMHYADYKFRLARSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSTKLFFDLFFSSFQGLLVAVLYCFLNKEVQAELLRRWRRWQEGKALQEERMASSHGSHMAPAGTCHGDPCEKLQLMSAGSSSGTGCEPSAKTSLASSLPRLADSPT

Target ORF information:

RefSeq Version	NM_172092.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucagon receptor (Gcgr), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172092.2

ORF Insert Sequence:

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGCCAAAGG CACCCTCTGC CCAGGTAATG GACTTTTTGT TTGAGAAGTG GAAGCTCTAT 
AGTGACCAGT GCCACCACAA CCTAAGCCTG CTGCCCCCAC CTACTGAGCT GGTCTGCAAC 
AGAACTTTCG ACAAGTACTC CTGCTGGCCT GACACCCCTC CCAACACCAC TGCCAACATT 
TCCTGCCCCT GGTACCTACC TTGGTACCAC AAAGTGCAGC ACCGCCTAGT GTTCAAGAGG 
TGTGGGCCTG ATGGGCAGTG GGTTCGAGGG CCACGGGGGC AGTCATGGCG CGACGCCTCC 
CAATGTCAGA TGGATGATGA CGAGATCGAG GTCCAGAAGG GGGTAGCCAA GATGTATAGC 
AGCTACCAGG TGATGTACAC TGTGGGCTAC AGTCTGTCCC TGGGGGCCTT GCTCCTGGCG 
CTGGTCATCC TGCTGGGCCT CAGGAAGCTG CACTGCACCC GGAACTACAT CCACGGGAAC 
CTGTTCGCGT CCTTCGTGCT CAAGGCTGGC TCTGTGCTGG TCATTGATTG GCTGCTCAAG 
ACACGCTATA GCCAGAAGAT TGGAGATGAC CTCAGTGTGA GCGTCTGGCT CAGTGATGGG 
GCGGTGGCTG GCTGCAGAGT GGCCACAGTG ATCATGCAGT ACGGCATCAT AGCCAACTAC 
TGCTGGTTGC TGGTGGAGGG TGTGTACCTG TACAGCCTGC TGAGCATCAC CACCTTCTCG 
GAGAAGAGCT TCTTCTCCCT CTATCTGTGC ATCGGCTGGG GATCTCCCCT GCTGTTTGTC 
ATCCCCTGGG TGGTGGTCAA GTGTCTGTTT GAGAATGTCC AGTGCTGGAC CAGCAATGAC 
AATATGGGAT TCTGGTGGAT CCTGCGTATC CCTGTACTCC TGGCCATACT GATCAATTTT 
TTCATCTTTG TCCGCATCAT TCATCTTCTT GTGGCCAAGC TGCGTGCCCA TCAGATGCAC 
TATGCTGATT ACAAGTTCCG GCTAGCCAGG TCCACGCTGA CCCTCATTCC TCTGCTGGGA 
GTCCACGAAG TGGTCTTTGC CTTTGTGACT GATGAGCATG CCCAGGGCAC CCTGCGCTCC 
ACCAAGCTCT TTTTTGACCT GTTCTTCAGC TCCTTCCAGG GTCTGCTGGT GGCTGTTCTC 
TACTGTTTCC TCAACAAGGA GGTGCAGGCA GAGCTACTGC GGCGTTGGAG GCGATGGCAA 
GAAGGCAAAG CTCTTCAGGA GGAAAGGATG GCCAGCAGCC ATGGCAGCCA CATGGCCCCA 
GCAGGGACTT GTCATGGTGA TCCCTGTGAG AAACTTCAGC TTATGAGTGC AGGCAGCAGC 
AGTGGGACTG GCTGTGAGCC CTCTGCGAAG ACCTCATTGG CCAGTAGTCT CCCAAGGCTG 
GCTGACAGCC CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcgr ( XM_006247876.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247938.1 Rattus norvegicus glucagon receptor (Gcgr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12671
Clone ID Related Accession (Same CDS sequence)	NM_172091.2 , XM_006247876.3 , NM_172092.2
Accession Version	XM_006247876.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glucagon receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247876.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGCCAAAGG CACCCTCTGC CCAGGTAATG GACTTTTTGT TTGAGAAGTG GAAGCTCTAT 
AGTGACCAGT GCCACCACAA CCTAAGCCTG CTGCCCCCAC CTACTGAGCT GGTCTGCAAC 
AGAACTTTCG ACAAGTACTC CTGCTGGCCT GACACCCCTC CCAACACCAC TGCCAACATT 
TCCTGCCCCT GGTACCTACC TTGGTACCAC AAAGTGCAGC ACCGCCTAGT GTTCAAGAGG 
TGTGGGCCTG ATGGGCAGTG GGTTCGAGGG CCACGGGGGC AGTCATGGCG CGACGCCTCC 
CAATGTCAGA TGGATGATGA CGAGATCGAG GTCCAGAAGG GGGTAGCCAA GATGTATAGC 
AGCTACCAGG TGATGTACAC TGTGGGCTAC AGTCTGTCCC TGGGGGCCTT GCTCCTGGCG 
CTGGTCATCC TGCTGGGCCT CAGGAAGCTG CACTGCACCC GGAACTACAT CCACGGGAAC 
CTGTTCGCGT CCTTCGTGCT CAAGGCTGGC TCTGTGCTGG TCATTGATTG GCTGCTCAAG 
ACACGCTATA GCCAGAAGAT TGGAGATGAC CTCAGTGTGA GCGTCTGGCT CAGTGATGGG 
GCGGTGGCTG GCTGCAGAGT GGCCACAGTG ATCATGCAGT ACGGCATCAT AGCCAACTAC 
TGCTGGTTGC TGGTGGAGGG TGTGTACCTG TACAGCCTGC TGAGCATCAC CACCTTCTCG 
GAGAAGAGCT TCTTCTCCCT CTATCTGTGC ATCGGCTGGG GATCTCCCCT GCTGTTTGTC 
ATCCCCTGGG TGGTGGTCAA GTGTCTGTTT GAGAATGTCC AGTGCTGGAC CAGCAATGAC 
AATATGGGAT TCTGGTGGAT CCTGCGTATC CCTGTACTCC TGGCCATACT GATCAATTTT 
TTCATCTTTG TCCGCATCAT TCATCTTCTT GTGGCCAAGC TGCGTGCCCA TCAGATGCAC 
TATGCTGATT ACAAGTTCCG GCTAGCCAGG TCCACGCTGA CCCTCATTCC TCTGCTGGGA 
GTCCACGAAG TGGTCTTTGC CTTTGTGACT GATGAGCATG CCCAGGGCAC CCTGCGCTCC 
ACCAAGCTCT TTTTTGACCT GTTCTTCAGC TCCTTCCAGG GTCTGCTGGT GGCTGTTCTC 
TACTGTTTCC TCAACAAGGA GGTGCAGGCA GAGCTACTGC GGCGTTGGAG GCGATGGCAA 
GAAGGCAAAG CTCTTCAGGA GGAAAGGATG GCCAGCAGCC ATGGCAGCCA CATGGCCCCA 
GCAGGGACTT GTCATGGTGA TCCCTGTGAG AAACTTCAGC TTATGAGTGC AGGCAGCAGC 
AGTGGGACTG GCTGTGAGCC CTCTGCGAAG ACCTCATTGG CCAGTAGTCT CCCAAGGCTG 
GCTGACAGCC CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247938.1
CDS	251..1708
Translation	MLLTQLHCPYLLLLLVVLSCLPKAPSAQVMDFLFEKWKLYSDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPPNTTANISCPWYLPWYHKVQHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAKMYSSYQVMYTVGYSLSLGALLLALVILLGLRKLHCTRNYIHGNLFASFVLKAGSVLVIDWLLKTRYSQKIGDDLSVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCIGWGSPLLFVIPWVVVKCLFENVQCWTSNDNMGFWWILRIPVLLAILINFFIFVRIIHLLVAKLRAHQMHYADYKFRLARSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSTKLFFDLFFSSFQGLLVAVLYCFLNKEVQAELLRRWRRWQEGKALQEERMASSHGSHMAPAGTCHGDPCEKLQLMSAGSSSGTGCEPSAKTSLASSLPRLADSPT

Target ORF information:

RefSeq Version	XM_006247876.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247876.3

ORF Insert Sequence:

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGCCAAAGG CACCCTCTGC CCAGGTAATG GACTTTTTGT TTGAGAAGTG GAAGCTCTAT 
AGTGACCAGT GCCACCACAA CCTAAGCCTG CTGCCCCCAC CTACTGAGCT GGTCTGCAAC 
AGAACTTTCG ACAAGTACTC CTGCTGGCCT GACACCCCTC CCAACACCAC TGCCAACATT 
TCCTGCCCCT GGTACCTACC TTGGTACCAC AAAGTGCAGC ACCGCCTAGT GTTCAAGAGG 
TGTGGGCCTG ATGGGCAGTG GGTTCGAGGG CCACGGGGGC AGTCATGGCG CGACGCCTCC 
CAATGTCAGA TGGATGATGA CGAGATCGAG GTCCAGAAGG GGGTAGCCAA GATGTATAGC 
AGCTACCAGG TGATGTACAC TGTGGGCTAC AGTCTGTCCC TGGGGGCCTT GCTCCTGGCG 
CTGGTCATCC TGCTGGGCCT CAGGAAGCTG CACTGCACCC GGAACTACAT CCACGGGAAC 
CTGTTCGCGT CCTTCGTGCT CAAGGCTGGC TCTGTGCTGG TCATTGATTG GCTGCTCAAG 
ACACGCTATA GCCAGAAGAT TGGAGATGAC CTCAGTGTGA GCGTCTGGCT CAGTGATGGG 
GCGGTGGCTG GCTGCAGAGT GGCCACAGTG ATCATGCAGT ACGGCATCAT AGCCAACTAC 
TGCTGGTTGC TGGTGGAGGG TGTGTACCTG TACAGCCTGC TGAGCATCAC CACCTTCTCG 
GAGAAGAGCT TCTTCTCCCT CTATCTGTGC ATCGGCTGGG GATCTCCCCT GCTGTTTGTC 
ATCCCCTGGG TGGTGGTCAA GTGTCTGTTT GAGAATGTCC AGTGCTGGAC CAGCAATGAC 
AATATGGGAT TCTGGTGGAT CCTGCGTATC CCTGTACTCC TGGCCATACT GATCAATTTT 
TTCATCTTTG TCCGCATCAT TCATCTTCTT GTGGCCAAGC TGCGTGCCCA TCAGATGCAC 
TATGCTGATT ACAAGTTCCG GCTAGCCAGG TCCACGCTGA CCCTCATTCC TCTGCTGGGA 
GTCCACGAAG TGGTCTTTGC CTTTGTGACT GATGAGCATG CCCAGGGCAC CCTGCGCTCC 
ACCAAGCTCT TTTTTGACCT GTTCTTCAGC TCCTTCCAGG GTCTGCTGGT GGCTGTTCTC 
TACTGTTTCC TCAACAAGGA GGTGCAGGCA GAGCTACTGC GGCGTTGGAG GCGATGGCAA 
GAAGGCAAAG CTCTTCAGGA GGAAAGGATG GCCAGCAGCC ATGGCAGCCA CATGGCCCCA 
GCAGGGACTT GTCATGGTGA TCCCTGTGAG AAACTTCAGC TTATGAGTGC AGGCAGCAGC 
AGTGGGACTG GCTGTGAGCC CTCTGCGAAG ACCTCATTGG CCAGTAGTCT CCCAAGGCTG 
GCTGACAGCC CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcgr ( XM_006247877.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247939.1 Rattus norvegicus glucagon receptor (Gcgr), transcript variant X4, mRNA.

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CloneID	ORa34025
Clone ID Related Accession (Same CDS sequence)	XM_006247877.2
Accession Version	XM_006247877.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glucagon receptor isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247877.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGAGCTGGT CTGCAACAGA ACTTTCGACA AGTACTCCTG CTGGCCTGAC ACCCCTCCCA 
ACACCACTGC CAACATTTCC TGCCCCTGGT ACCTACCTTG GTACCACAAA GCACCGCCTA 
GTGTTCAAGA GGTGTGGGCC TGATGGGCAG TGGGTTCGAG GGCCACGGGG GCAGTCATGG 
CGCGACGCCT CCCAATGTCA GATGGATGAT GACGAGATCG AGGTCCAGAA GGGGGTAGCC 
AAGATGTATA GCAGCTACCA GGTGATGTAC ACTGTGGGCT ACAGTCTGTC CCTGGGGGCC 
TTGCTCCTGG CGCTGGTCAT CCTGCTGGGC CTCAGGAAGC TGCACTGCAC CCGGAACTAC 
ATCCACGGGA ACCTGTTCGC GTCCTTCGTG CTCAAGGCTG GCTCTGTGCT GGTCATTGAT 
TGGCTGCTCA AGACACGCTA TAGCCAGAAG ATTGGAGATG ACCTCAGTGT GAGCGTCTGG 
CTCAGTGATG GGGCGGTGGC TGGCTGCAGA GTGGCCACAG TGATCATGCA GTACGGCATC 
ATAGCCAACT ACTGCTGGTT GCTGGTGGAG GGTGTGTACC TGTACAGCCT GCTGAGCATC 
ACCACCTTCT CGGAGAAGAG CTTCTTCTCC CTCTATCTGT GCATCGGCTG GGGATCTCCC 
CTGCTGTTTG TCATCCCCTG GGTGGTGGTC AAGTGTCTGT TTGAGAATGT CCAGTGCTGG 
ACCAGCAATG ACAATATGGG ATTCTGGTGG ATCCTGCGTA TCCCTGTACT CCTGGCCATA 
CTGATCAATT TTTTCATCTT TGTCCGCATC ATTCATCTTC TTGTGGCCAA GCTGCGTGCC 
CATCAGATGC ACTATGCTGA TTACAAGTTC CGGCTAGCCA GGTCCACGCT GACCCTCATT 
CCTCTGCTGG GAGTCCACGA AGTGGTCTTT GCCTTTGTGA CTGATGAGCA TGCCCAGGGC 
ACCCTGCGCT CCACCAAGCT CTTTTTTGAC CTGTTCTTCA GCTCCTTCCA GGGTCTGCTG 
GTGGCTGTTC TCTACTGTTT CCTCAACAAG GAGGTGCAGG CAGAGCTACT GCGGCGTTGG 
AGGCGATGGC AAGAAGGCAA AGCTCTTCAG GAGGAAAGGA TGGCCAGCAG CCATGGCAGC 
CACATGGCCC CAGCAGGGAC TTGTCATGGT GATCCCTGTG AGAAACTTCA GCTTATGAGT 
GCAGGCAGCA GCAGTGGGAC TGGCTGTGAG CCCTCTGCGA AGACCTCATT GGCCAGTAGT 
CTCCCAAGGC TGGCTGACAG CCCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247939.1
CDS	171..1520
Translation	MLLTQLHCPYLLLLLVVLSCLSWSATELSTSTPAGLTPLPTPLPTFPAPGTYLGTTKHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAKMYSSYQVMYTVGYSLSLGALLLALVILLGLRKLHCTRNYIHGNLFASFVLKAGSVLVIDWLLKTRYSQKIGDDLSVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCIGWGSPLLFVIPWVVVKCLFENVQCWTSNDNMGFWWILRIPVLLAILINFFIFVRIIHLLVAKLRAHQMHYADYKFRLARSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSTKLFFDLFFSSFQGLLVAVLYCFLNKEVQAELLRRWRRWQEGKALQEERMASSHGSHMAPAGTCHGDPCEKLQLMSAGSSSGTGCEPSAKTSLASSLPRLADSPT

Target ORF information:

RefSeq Version	XM_006247877.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247877.2

ORF Insert Sequence:

ATGCTCCTCA CCCAGCTCCA CTGTCCCTAC CTGCTGCTGC TGCTGGTGGT GCTGTCATGT 
CTGAGCTGGT CTGCAACAGA ACTTTCGACA AGTACTCCTG CTGGCCTGAC ACCCCTCCCA 
ACACCACTGC CAACATTTCC TGCCCCTGGT ACCTACCTTG GTACCACAAA GCACCGCCTA 
GTGTTCAAGA GGTGTGGGCC TGATGGGCAG TGGGTTCGAG GGCCACGGGG GCAGTCATGG 
CGCGACGCCT CCCAATGTCA GATGGATGAT GACGAGATCG AGGTCCAGAA GGGGGTAGCC 
AAGATGTATA GCAGCTACCA GGTGATGTAC ACTGTGGGCT ACAGTCTGTC CCTGGGGGCC 
TTGCTCCTGG CGCTGGTCAT CCTGCTGGGC CTCAGGAAGC TGCACTGCAC CCGGAACTAC 
ATCCACGGGA ACCTGTTCGC GTCCTTCGTG CTCAAGGCTG GCTCTGTGCT GGTCATTGAT 
TGGCTGCTCA AGACACGCTA TAGCCAGAAG ATTGGAGATG ACCTCAGTGT GAGCGTCTGG 
CTCAGTGATG GGGCGGTGGC TGGCTGCAGA GTGGCCACAG TGATCATGCA GTACGGCATC 
ATAGCCAACT ACTGCTGGTT GCTGGTGGAG GGTGTGTACC TGTACAGCCT GCTGAGCATC 
ACCACCTTCT CGGAGAAGAG CTTCTTCTCC CTCTATCTGT GCATCGGCTG GGGATCTCCC 
CTGCTGTTTG TCATCCCCTG GGTGGTGGTC AAGTGTCTGT TTGAGAATGT CCAGTGCTGG 
ACCAGCAATG ACAATATGGG ATTCTGGTGG ATCCTGCGTA TCCCTGTACT CCTGGCCATA 
CTGATCAATT TTTTCATCTT TGTCCGCATC ATTCATCTTC TTGTGGCCAA GCTGCGTGCC 
CATCAGATGC ACTATGCTGA TTACAAGTTC CGGCTAGCCA GGTCCACGCT GACCCTCATT 
CCTCTGCTGG GAGTCCACGA AGTGGTCTTT GCCTTTGTGA CTGATGAGCA TGCCCAGGGC 
ACCCTGCGCT CCACCAAGCT CTTTTTTGAC CTGTTCTTCA GCTCCTTCCA GGGTCTGCTG 
GTGGCTGTTC TCTACTGTTT CCTCAACAAG GAGGTGCAGG CAGAGCTACT GCGGCGTTGG 
AGGCGATGGC AAGAAGGCAA AGCTCTTCAG GAGGAAAGGA TGGCCAGCAG CCATGGCAGC 
CACATGGCCC CAGCAGGGAC TTGTCATGGT GATCCCTGTG AGAAACTTCA GCTTATGAGT 
GCAGGCAGCA GCAGTGGGAC TGGCTGTGAG CCCTCTGCGA AGACCTCATT GGCCAGTAGT 
CTCCCAAGGC TGGCTGACAG CCCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcgr ( XM_008768468.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766690.1 Rattus norvegicus glucagon receptor (Gcgr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42995
Clone ID Related Accession (Same CDS sequence)	XM_008768468.2
Accession Version	XM_008768468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1473bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glucagon receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG GCACTGGACC CTCGCTCTCT TTATCTGAGC CTGAGGAGAG GTGCACACAC 
CCCCAAGGAC CCAGGCACCC AACCTCTGCC AGATGTGGGG GGGTGGCTAC CCAGAGGCAT 
GCTCCTCACC CAGCTCCACT GTCCCTACCT GCTGCTGCTG CTGGTGGTGC TGTCATGTCT 
GAGCTGGTCT GCAACAGAAC TTTCGACAAG TACTCCTGCT GGCCTGACAC CCCTCCCAAC 
ACCACTGCCA ACATTTCCTG CCCCTGGTAC CTACCTTGGT ACCACAAAGT GCAGCACCGC 
CTAGTGTTCA AGAGGTGTGG GCCTGATGGG CAGTGGGTTC GAGGGCCACG GGGGCAGTCA 
TGGCGCGACG CCTCCCAATG TCAGATGGAT GATGACGAGA TCGAGGTCCA GAAGGGGGTA 
GCCAAGATGT ATAGCAGCTA CCAGGTGATG TACACTGTGG GCTACAGTCT GTCCCTGGGG 
GCCTTGCTCC TGGCGCTGGT CATCCTGCTG GGCCTCAGGA AGCTGCACTG CACCCGGAAC 
TACATCCACG GGAACCTGTT CGCGTCCTTC GTGCTCAAGG CTGGCTCTGT GCTGGTCATT 
GATTGGCTGC TCAAGACACG CTATAGCCAG AAGATTGGAG ATGACCTCAG TGTGAGCGTC 
TGGCTCAGTG ATGGGGCGGT GGCTGGCTGC AGAGTGGCCA CAGTGATCAT GCAGTACGGC 
ATCATAGCCA ACTACTGCTG GTTGCTGGTG GAGGGTGTGT ACCTGTACAG CCTGCTGAGC 
ATCACCACCT TCTCGGAGAA GAGCTTCTTC TCCCTCTATC TGTGCATCGG CTGGGGATCT 
CCCCTGCTGT TTGTCATCCC CTGGGTGGTG GTCAAGTGTC TGTTTGAGAA TGTCCAGTGC 
TGGACCAGCA ATGACAATAT GGGATTCTGG TGGATCCTGC GTATCCCTGT ACTCCTGGCC 
ATACTGATCA ATTTTTTCAT CTTTGTCCGC ATCATTCATC TTCTTGTGGC CAAGCTGCGT 
GCCCATCAGA TGCACTATGC TGATTACAAG TTCCGGCTAG CCAGGTCCAC GCTGACCCTC 
ATTCCTCTGC TGGGAGTCCA CGAAGTGGTC TTTGCCTTTG TGACTGATGA GCATGCCCAG 
GGCACCCTGC GCTCCACCAA GCTCTTTTTT GACCTGTTCT TCAGCTCCTT CCAGGGTCTG 
CTGGTGGCTG TTCTCTACTG TTTCCTCAAC AAGGAGGTGC AGGCAGAGCT ACTGCGGCGT 
TGGAGGCGAT GGCAAGAAGG CAAAGCTCTT CAGGAGGAAA GGATGGCCAG CAGCCATGGC 
AGCCACATGG CCCCAGCAGG GACTTGTCAT GGTGATCCCT GTGAGAAACT TCAGCTTATG 
AGTGCAGGCA GCAGCAGTGG GACTGGCTGT GAGCCCTCTG CGAAGACCTC ATTGGCCAGT 
AGTCTCCCAA GGCTGGCTGA CAGCCCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766690.1
CDS	179..1651
Translation	MDLGTGPSLSLSEPEERCTHPQGPRHPTSARCGGVATQRHAPHPAPLSLPAAAAGGAVMSELVCNRTFDKYSCWPDTPPNTTANISCPWYLPWYHKVQHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAKMYSSYQVMYTVGYSLSLGALLLALVILLGLRKLHCTRNYIHGNLFASFVLKAGSVLVIDWLLKTRYSQKIGDDLSVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCIGWGSPLLFVIPWVVVKCLFENVQCWTSNDNMGFWWILRIPVLLAILINFFIFVRIIHLLVAKLRAHQMHYADYKFRLARSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSTKLFFDLFFSSFQGLLVAVLYCFLNKEVQAELLRRWRRWQEGKALQEERMASSHGSHMAPAGTCHGDPCEKLQLMSAGSSSGTGCEPSAKTSLASSLPRLADSPT

Target ORF information:

RefSeq Version	XM_008768468.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768468.2

ORF Insert Sequence:

ATGGACCTGG GCACTGGACC CTCGCTCTCT TTATCTGAGC CTGAGGAGAG GTGCACACAC 
CCCCAAGGAC CCAGGCACCC AACCTCTGCC AGATGTGGGG GGGTGGCTAC CCAGAGGCAT 
GCTCCTCACC CAGCTCCACT GTCCCTACCT GCTGCTGCTG CTGGTGGTGC TGTCATGTCT 
GAGCTGGTCT GCAACAGAAC TTTCGACAAG TACTCCTGCT GGCCTGACAC CCCTCCCAAC 
ACCACTGCCA ACATTTCCTG CCCCTGGTAC CTACCTTGGT ACCACAAAGT GCAGCACCGC 
CTAGTGTTCA AGAGGTGTGG GCCTGATGGG CAGTGGGTTC GAGGGCCACG GGGGCAGTCA 
TGGCGCGACG CCTCCCAATG TCAGATGGAT GATGACGAGA TCGAGGTCCA GAAGGGGGTA 
GCCAAGATGT ATAGCAGCTA CCAGGTGATG TACACTGTGG GCTACAGTCT GTCCCTGGGG 
GCCTTGCTCC TGGCGCTGGT CATCCTGCTG GGCCTCAGGA AGCTGCACTG CACCCGGAAC 
TACATCCACG GGAACCTGTT CGCGTCCTTC GTGCTCAAGG CTGGCTCTGT GCTGGTCATT 
GATTGGCTGC TCAAGACACG CTATAGCCAG AAGATTGGAG ATGACCTCAG TGTGAGCGTC 
TGGCTCAGTG ATGGGGCGGT GGCTGGCTGC AGAGTGGCCA CAGTGATCAT GCAGTACGGC 
ATCATAGCCA ACTACTGCTG GTTGCTGGTG GAGGGTGTGT ACCTGTACAG CCTGCTGAGC 
ATCACCACCT TCTCGGAGAA GAGCTTCTTC TCCCTCTATC TGTGCATCGG CTGGGGATCT 
CCCCTGCTGT TTGTCATCCC CTGGGTGGTG GTCAAGTGTC TGTTTGAGAA TGTCCAGTGC 
TGGACCAGCA ATGACAATAT GGGATTCTGG TGGATCCTGC GTATCCCTGT ACTCCTGGCC 
ATACTGATCA ATTTTTTCAT CTTTGTCCGC ATCATTCATC TTCTTGTGGC CAAGCTGCGT 
GCCCATCAGA TGCACTATGC TGATTACAAG TTCCGGCTAG CCAGGTCCAC GCTGACCCTC 
ATTCCTCTGC TGGGAGTCCA CGAAGTGGTC TTTGCCTTTG TGACTGATGA GCATGCCCAG 
GGCACCCTGC GCTCCACCAA GCTCTTTTTT GACCTGTTCT TCAGCTCCTT CCAGGGTCTG 
CTGGTGGCTG TTCTCTACTG TTTCCTCAAC AAGGAGGTGC AGGCAGAGCT ACTGCGGCGT 
TGGAGGCGAT GGCAAGAAGG CAAAGCTCTT CAGGAGGAAA GGATGGCCAG CAGCCATGGC 
AGCCACATGG CCCCAGCAGG GACTTGTCAT GGTGATCCCT GTGAGAAACT TCAGCTTATG 
AGTGCAGGCA GCAGCAGTGG GACTGGCTGT GAGCCCTCTG CGAAGACCTC ATTGGCCAGT 
AGTCTCCCAA GGCTGGCTGA CAGCCCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gcgr ( XM_008768469.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766691.1 Rattus norvegicus glucagon receptor (Gcgr), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa42996
Clone ID Related Accession (Same CDS sequence)	XM_008768469.2
Accession Version	XM_008768469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glucagon receptor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG GCACTGGACC CTCGCTCTCT TTATCTGAGC CTGAGGAGAG GTGCACACAC 
CCCCAAGGAC CCAGGCACCC AACCTCTGCC AGATGTGGGG GGGTGGCTAC CCAGAGGCAT 
GCTCCTCACC CAGCTCCACT GTCCCTACCT GCTGCTGCTG CTGGTGGTGC TGTCATGTCT 
GAGCTGGTCT GCAACAGAAC TTTCGACAAG TACTCCTGCT GGCCTGACAC CCCTCCCAAC 
ACCACTGCCA ACATTTCCTG CCCCTGGTAC CTACCTTGGT ACCACAAAGT GCAGCACCGC 
CTAGTGTTCA AGAGGTGTGG GCCTGATGGG CAGTGGGTTC GAGGGCCACG GGGGCAGTCA 
TGGCGCGACG CCTCCCAATG TCAGATGGAT GATGACGAGA TCGAGGTCCA GAAGGGGGTA 
GCCAAGATGT ATAGCAGCTA CCAGGTGATG TACACTGTGG GCTACAGTCT GTCCCTGGGG 
GCCTTGCTCC TGGCGCTGGT CATCCTGCTG GGCCTCAGGA AGCTGCACTG CACCCGGAAC 
TACATCCACG GGAACCTGTT CGCGTCCTTC GTGCTCAAGG CTGGCTCTGT GCTGGTCATT 
GATTGGCTGC TCAAGACACG CTATAGCCAG AAGATTGGAG ATGACCTCAG TGTGAGCGTC 
TGGCTCAGTG ATGGGGCGGT GGCTGGCTGC AGAGTGGCCA CAGTGATCAT GCAGTACGGC 
ATCATAGCCA ACTACTGCTG GTTGCTGGTG GAGGGTGTGT ACCTGTACAG CCTGCTGAGC 
ATCACCACCT TCTCGGAGAA GAGCTTCTTC TCCCTCTATC TGTGCATCGG CTGGGGATCT 
CCCCTGCTGT TTGTCATCCC CTGGGTGGTG GTCAAGTGTC TGTTTGAGAA TGTCCAGTGC 
TGGACCAGCA ATGACAATAT GGGATTCTGG TGGATCCTGC GTATCCCTGT ACTCCTGGCC 
ATACTGATCA ATTTTTTCAT CTTTGTCCGC ATCATTCATC TTCTTGTGGC CAAGCTGCGT 
GCCCATCAGA TGCACTATGC TGATTACAAG TTCCGGCTAG CCAGGTCCAC GCTGACCCTC 
ATTCCTCTGC TGGGAGTCCA CGAAGTGGTC TTTGCCTTTG TGACTGATGA GCATGCCCAG 
GGCACCCTGC GCTCCACCAA GCTCTTTTTT GACCTGTTCT TCAGCTCCTT CCAGGGTCTG 
CTGGTGGCTG TTCTCTACTG TTTCCTCAAC AAGGAGTTGC ATGCGTCGGA TGTCAGGTCC 
CCTAGGTCCT CAGGGAGCAG GGTTATGAGA TGGTCCCCGC CTTTCCTGGT GACAAGTGGC 
CCTGCTGAAC CCAATGTAAA ACTTGCCTTG CTTTGGGTCT GCTTAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766691.1
CDS	182..1552
Translation	MDLGTGPSLSLSEPEERCTHPQGPRHPTSARCGGVATQRHAPHPAPLSLPAAAAGGAVMSELVCNRTFDKYSCWPDTPPNTTANISCPWYLPWYHKVQHRLVFKRCGPDGQWVRGPRGQSWRDASQCQMDDDEIEVQKGVAKMYSSYQVMYTVGYSLSLGALLLALVILLGLRKLHCTRNYIHGNLFASFVLKAGSVLVIDWLLKTRYSQKIGDDLSVSVWLSDGAVAGCRVATVIMQYGIIANYCWLLVEGVYLYSLLSITTFSEKSFFSLYLCIGWGSPLLFVIPWVVVKCLFENVQCWTSNDNMGFWWILRIPVLLAILINFFIFVRIIHLLVAKLRAHQMHYADYKFRLARSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSTKLFFDLFFSSFQGLLVAVLYCFLNKELHASDVRSPRSSGSRVMRWSPPFLVTSGPAEPNVKLALLWVCLD

Target ORF information:

RefSeq Version	XM_008768469.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glucagon receptor (Gcgr), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768469.2

ORF Insert Sequence:

ATGGACCTGG GCACTGGACC CTCGCTCTCT TTATCTGAGC CTGAGGAGAG GTGCACACAC 
CCCCAAGGAC CCAGGCACCC AACCTCTGCC AGATGTGGGG GGGTGGCTAC CCAGAGGCAT 
GCTCCTCACC CAGCTCCACT GTCCCTACCT GCTGCTGCTG CTGGTGGTGC TGTCATGTCT 
GAGCTGGTCT GCAACAGAAC TTTCGACAAG TACTCCTGCT GGCCTGACAC CCCTCCCAAC 
ACCACTGCCA ACATTTCCTG CCCCTGGTAC CTACCTTGGT ACCACAAAGT GCAGCACCGC 
CTAGTGTTCA AGAGGTGTGG GCCTGATGGG CAGTGGGTTC GAGGGCCACG GGGGCAGTCA 
TGGCGCGACG CCTCCCAATG TCAGATGGAT GATGACGAGA TCGAGGTCCA GAAGGGGGTA 
GCCAAGATGT ATAGCAGCTA CCAGGTGATG TACACTGTGG GCTACAGTCT GTCCCTGGGG 
GCCTTGCTCC TGGCGCTGGT CATCCTGCTG GGCCTCAGGA AGCTGCACTG CACCCGGAAC 
TACATCCACG GGAACCTGTT CGCGTCCTTC GTGCTCAAGG CTGGCTCTGT GCTGGTCATT 
GATTGGCTGC TCAAGACACG CTATAGCCAG AAGATTGGAG ATGACCTCAG TGTGAGCGTC 
TGGCTCAGTG ATGGGGCGGT GGCTGGCTGC AGAGTGGCCA CAGTGATCAT GCAGTACGGC 
ATCATAGCCA ACTACTGCTG GTTGCTGGTG GAGGGTGTGT ACCTGTACAG CCTGCTGAGC 
ATCACCACCT TCTCGGAGAA GAGCTTCTTC TCCCTCTATC TGTGCATCGG CTGGGGATCT 
CCCCTGCTGT TTGTCATCCC CTGGGTGGTG GTCAAGTGTC TGTTTGAGAA TGTCCAGTGC 
TGGACCAGCA ATGACAATAT GGGATTCTGG TGGATCCTGC GTATCCCTGT ACTCCTGGCC 
ATACTGATCA ATTTTTTCAT CTTTGTCCGC ATCATTCATC TTCTTGTGGC CAAGCTGCGT 
GCCCATCAGA TGCACTATGC TGATTACAAG TTCCGGCTAG CCAGGTCCAC GCTGACCCTC 
ATTCCTCTGC TGGGAGTCCA CGAAGTGGTC TTTGCCTTTG TGACTGATGA GCATGCCCAG 
GGCACCCTGC GCTCCACCAA GCTCTTTTTT GACCTGTTCT TCAGCTCCTT CCAGGGTCTG 
CTGGTGGCTG TTCTCTACTG TTTCCTCAAC AAGGAGTTGC ATGCGTCGGA TGTCAGGTCC 
CCTAGGTCCT CAGGGAGCAG GGTTATGAGA TGGTCCCCGC CTTTCCTGGT GACAAGTGGC 
CCTGCTGAAC CCAATGTAAA ACTTGCCTTG CTTTGGGTCT GCTTAGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure of the full-length glucagon class B G-protein-coupled receptor.
Nature546(7657)259-264(2017 06)
Zhang H,Qiao A,Yang D,Yang L,Dai A,de Graaf C,Reedtz-Runge S,Dharmarajan V,Zhang H,Han GW,Grant TD,Sierra RG,Weierstall U,Nelson G,Liu W,Wu Y,Ma L,Cai X,Lin G,Wu X,Geng Z,Dong Y,Song G,Griffin PR,Lau J,Cherezov V,Yang H,Hanson MA,Stevens RC,Zhao Q,Jiang H,Wang MW,Wu B

Vasodilator Effect of Glucagon: Receptorial Crosstalk Among Glucagon, GLP-1, and Receptor for Glucagon and GLP-1.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme48(7)476-83(2016 Jul)
S?lley E,Kun S,Szij?rt? IA,Kert?sz M,Wittmann I,Moln?r GA

Liver glucagon receptors (GluR): effect of exercise and fasting on binding characteristics, GluR-mRNA, and GluR protein content in rats.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(10)716-21(2013 Sep)
Melan?on A,Gagnon V,Milot M,Charest ?,Foucher D,P?ronnet F,Unson O'Brien CG,Asselin E,Lavoie C

Glucagon receptor recycling: role of carboxyl terminus, beta-arrestins, and cytoskeleton.
American journal of physiology. Cell physiology295(5)C1230-7(2008 Nov)
Krilov L,Nguyen A,Miyazaki T,Unson CG,Bouscarel B

The glucagon receptor is required for the adaptive metabolic response to fasting.
Cell metabolism8(5)359-71(2008 Nov)
Longuet C,Sinclair EM,Maida A,Baggio LL,Maziarz M,Charron MJ,Drucker DJ

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Gcgr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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