Yy1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Yy1
Entrez Gene ID	24919
Full Name	YY1 transcription factor
Synonyms	NF-E1,NMP-1,NMP1,UCRBP
General protein information	Preferred Names YY1 transcription factor Names transcriptional repressor protein YY1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q32
Summary	acts as a transcriptional repressor of the steroidogenic acute regulatory (StaR) protein; decreases SREBP-1a binding to a sterol regulatory element [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240537.3	XP_006240599.1	transcriptional repressor protein YY1 isoform X1
NM_173290.1	NP_775412.1	transcriptional repressor protein YY1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	YY1	NP_001026381.1
Bos taurus (cattle)	YY1	NP_001091550.1
Rattus norvegicus (Norway rat)	Yy1	NP_775412.1
Danio rerio (zebrafish)	yy1a	NP_997782.1
Xenopus tropicalis (tropical clawed frog)	yy1	NP_001116880.1
Mus musculus (house mouse)	Yy1	NP_033563.2
Canis lupus familiaris (dog)	YY1	XP_854514.1
Homo sapiens (human)	YY1	NP_003394.1
Pan troglodytes (chimpanzee)	YY1	XP_510162.3

Gene Ontology
Bibliography

Related articles in PubMed

An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation.
Mandal K, Bader SL, Kumar P, Malakar D, Campbell DS, Pradhan BS, Sarkar RK, Wadhwa N, Sensharma S, Jain V, Moritz RL, Majumdar SS
DNA research : an international journal for rapid publication of reports on genes and genomes24(2)143-157(2017 Apr)

Transcriptional Regulation of the Astrocytic Excitatory Amino Acid Transporter 1 (EAAT1) via NF-?B and Yin Yang 1 (YY1).
Karki P, Kim C, Smith K, Son DS, Aschner M, Lee E
The Journal of biological chemistry290(39)23725-37(2015 Sep)

Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis.
Tian FJ, An LN, Wang GK, Zhu JQ, Li Q, Zhang YY, Zeng A, Zou J, Zhu RF, Han XS, Shen N, Yang HT, Zhao XX, Huang S, Qin YW, Jing Q
Cardiovascular research103(1)100-10(2014 Jul)

Yin Yang 1 is a repressor of glutamate transporter EAAT2, and it mediates manganese-induced decrease of EAAT2 expression in astrocytes.
Karki P, Webb A, Smith K, Johnson J Jr, Lee K, Son DS, Aschner M, Lee E
Molecular and cellular biology34(7)1280-9(2014 Apr)

The primary microRNA-208b interacts with Polycomb-group protein, Ezh2, to regulate gene expression in the heart.
Mathiyalagan P, Okabe J, Chang L, Su Y, Du XJ, El-Osta A
Nucleic acids research42(2)790-803(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggest that NF-kappaB is a critical positive regulator of EAAT1, mediating the stimulatory effects of EGF, whereas YY1 is a negative regulator of EAAT1 with HDACs as co-repressors
Title: Transcriptional Regulation of the Astrocytic Excitatory Amino Acid Transporter 1 (EAAT1) via NF-?B and Yin Yang 1 (YY1).

YY1, with HDACs as corepressors, is a critical negative transcriptional regulator of EAAT2 and mediates Mn-induced EAAT2 repression.
Title: Yin Yang 1 is a repressor of glutamate transporter EAAT2, and it mediates manganese-induced decrease of EAAT2 expression in astrocytes.

roles of poly(ADP-ribose) polymerase-1 (PARP-1) and transcription factor Yin Yang 1 (YY1) in Cxcl12 transcription
Title: PARP-1 and YY1 are important novel regulators of CXCL12 gene transcription in rat pancreatic beta cells.

These data suggest that the inability of YY1 to access the hypermethylated promoter may be one of the mechanisms that mediate vimentin downregulation.
Title: CpG methylation prevents YY1-mediated transcriptional activation of the vimentin promoter.

HO-1 functionally interplays with the multifunctional transcription factor YY1 and this interplay explains some of the protective activities of HO-1.
Title: Interplay between heme oxygenase-1 and the multifunctional transcription factor yin yang 1 in the inhibition of intimal hyperplasia.

The following Yy1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Yy1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13491	NM_173290.1 Latest version!	Rattus norvegicus YY1 transcription factor (Yy1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
ORa29605	XM_006240537.3 Latest version!	Rattus norvegicus YY1 transcription factor (Yy1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Yy1 ( NM_173290.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775412.1 Rattus norvegicus YY1 transcription factor (Yy1), mRNA.

Price & Availability↑

CloneID	ORa13491
Clone ID Related Accession (Same CDS sequence)	NM_173290.1
Accession Version	NM_173290.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional repressor protein YY1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB080317.1. On Aug 13, 2006 this sequence version replaced XM_001069615.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB080317.1, EV778701.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCGG GCGACACCCT CTACATTGCC ACGGACGGCT CGGAGATGCC AGCCGAGATC 
GTGGAACTGC ATGAGATTGA GGTGGAGACC ATCCCGGTGG AGACTATCGA GACCACGGTG 
GTGGGCGAGG AGGAGGACGA CGACGAAGAC GACGAGGATG GTGGCGGCGG AGACCACGGT 
GGCGGGGGCG GCCACGGGCA CGCTGGCCAC CACCATCACC ACCACCACCA CCACCACCCG 
CCCATGATCG CGCTGCAGCC GCTGGTCACC GACGACCCGA CCCAAGTGCA CCACCACCAA 
GAGGTGATTC TGGTGCAGAC GCGCGAGGAG GTAGTGGGTG GCGACGACTC GGACGGGCTG 
CGCGCCGAGG ACGGGTTCGA GGACCAGATC CTCATTCCGG TACCCGCGCC GGCCGGCGGA 
GACGACGACT ACATCGAGCA GACGCTGGTC ACCGTGGCGG CGGCCGGCAA GAGCGGTGGC 
GGGTCTTCGT CGGGCGGCGG CCGCGTTAAG AAGGGCGGCG GCAAGAAGAG TGGCAAGAAG 
AGTTACCTGG GCAGCGGGGC CGGCGCGGCG GGCGGTGGCG GCGCCGACCC GGGTAATAAG 
AAGTGGGAAC AGAAGCAGGT GCAGATCAAG ACCCTGGAGG GCGAGTTCTC GGTCACCATG 
TGGTCTTCAG ATGAAAAAAA AGATATTGAC CATGAAACAG TGGTTGAAGA GCAGATCATT 
GGGGAGAACT CACCTCCTGA TTATTCTGAA TATATGACAG GCAAGAAACT CCCTCCTGGA 
GGGATACCTG GCATTGACCT CTCAGACCCC AAGCAACTGG CAGAATTTGC CAGAATGAAG 
CCAAGAAAAA TTAAAGAAGA TGATGCTCCA AGAACAATAG CTTGCCCTCA TAAAGGCTGC 
ACAAAGATGT TCAGGGATAA CTCTGCTATG AGAAAGCATC TGCACACCCA CGGTCCCAGA 
GTCCACGTCT GTGCAGAATG TGGCAAAGCG TTCGTTGAGA GCTCAAAGCT AAAACGACAC 
CAGCTGGTTC ATACTGGAGA AAAGCCCTTT CAGTGCACAT TCGAAGGCTG CGGGAAGCGC 
TTTTCACTGG ACTTCAATTT GCGCACGCAT GTGCGAATCC ATACCGGAGA CAGGCCCTAT 
GTGTGCCCCT TCGACGGTTG TAATAAGAAG TTTGCTCAGT CAACTAACCT GAAATCTCAC 
ATCTTAACAC ACGCTAAAGC CAAAAACAAC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775412.1
CDS	1..1236
Translation	MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEDDDEDDEDGGGGDHGGGGGHGHAGHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGSSSGGGRVKKGGGKKSGKKSYLGSGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ

Target ORF information:

RefSeq Version	NM_173290.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus YY1 transcription factor (Yy1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173290.1

ORF Insert Sequence:

ATGGCCTCGG GCGACACCCT CTACATTGCC ACGGACGGCT CGGAGATGCC AGCCGAGATC 
GTGGAACTGC ATGAGATTGA GGTGGAGACC ATCCCGGTGG AGACTATCGA GACCACGGTG 
GTGGGCGAGG AGGAGGACGA CGACGAAGAC GACGAGGATG GTGGCGGCGG AGACCACGGT 
GGCGGGGGCG GCCACGGGCA CGCTGGCCAC CACCATCACC ACCACCACCA CCACCACCCG 
CCCATGATCG CGCTGCAGCC GCTGGTCACC GACGACCCGA CCCAAGTGCA CCACCACCAA 
GAGGTGATTC TGGTGCAGAC GCGCGAGGAG GTAGTGGGTG GCGACGACTC GGACGGGCTG 
CGCGCCGAGG ACGGGTTCGA GGACCAGATC CTCATTCCGG TACCCGCGCC GGCCGGCGGA 
GACGACGACT ACATCGAGCA GACGCTGGTC ACCGTGGCGG CGGCCGGCAA GAGCGGTGGC 
GGGTCTTCGT CGGGCGGCGG CCGCGTTAAG AAGGGCGGCG GCAAGAAGAG TGGCAAGAAG 
AGTTACCTGG GCAGCGGGGC CGGCGCGGCG GGCGGTGGCG GCGCCGACCC GGGTAATAAG 
AAGTGGGAAC AGAAGCAGGT GCAGATCAAG ACCCTGGAGG GCGAGTTCTC GGTCACCATG 
TGGTCTTCAG ATGAAAAAAA AGATATTGAC CATGAAACAG TGGTTGAAGA GCAGATCATT 
GGGGAGAACT CACCTCCTGA TTATTCTGAA TATATGACAG GCAAGAAACT CCCTCCTGGA 
GGGATACCTG GCATTGACCT CTCAGACCCC AAGCAACTGG CAGAATTTGC CAGAATGAAG 
CCAAGAAAAA TTAAAGAAGA TGATGCTCCA AGAACAATAG CTTGCCCTCA TAAAGGCTGC 
ACAAAGATGT TCAGGGATAA CTCTGCTATG AGAAAGCATC TGCACACCCA CGGTCCCAGA 
GTCCACGTCT GTGCAGAATG TGGCAAAGCG TTCGTTGAGA GCTCAAAGCT AAAACGACAC 
CAGCTGGTTC ATACTGGAGA AAAGCCCTTT CAGTGCACAT TCGAAGGCTG CGGGAAGCGC 
TTTTCACTGG ACTTCAATTT GCGCACGCAT GTGCGAATCC ATACCGGAGA CAGGCCCTAT 
GTGTGCCCCT TCGACGGTTG TAATAAGAAG TTTGCTCAGT CAACTAACCT GAAATCTCAC 
ATCTTAACAC ACGCTAAAGC CAAAAACAAC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Yy1 ( XM_006240537.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240599.1 Rattus norvegicus YY1 transcription factor (Yy1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29605
Clone ID Related Accession (Same CDS sequence)	XM_006240537.3
Accession Version	XM_006240537.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional repressor protein YY1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240537.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG GCGACACCCT CTACATTGCC ACGGACGGCT CGGAGATGCC AGCCGAGATC 
GTGGAACTGC ATGAGATTGA GGTGGAGACC ATCCCGGTGG AGACTATCGA GACCACGGTG 
GTGGGCGAGG AGGAGGACGA CGACGAAGAC GACGAGGATG GTGGCGGCGG AGACCACGGT 
GGCGGGGGCG GCCACGGGCA CGCTGGCCAC CACCATCACC ACCACCACCA CCACCACCCG 
CCCATGATCG CGCTGCAGCC GCTGGTCACC GACGACCCGA CCCAAGTGCA CCACCACCAA 
GAGGTGATTC TGGTGCAGAC GCGCGAGGAG GTAGTGGGTG GCGACGACTC GGACGGGCTG 
CGCGCCGAGG ACGGGTTCGA GGACCAGATC CTCATTCCGG TACCCGCGCC GGCCGGCGGA 
GACGACGACT ACATCGAGCA GACGCTGGTC ACCGTGGCGG CGGCCGGCAA GAGCGGTGGC 
GGGTCTTCGT CGGGCGGCGG CCGCGTTAAG AAGGGCGGCG GCAAGAAGAG TGGCAAGAAG 
AGTTACCTGG GCAGCGGGGC CGGCGCGGCG GGCGGTGGCG GCGCCGACCC GGGTAATAAG 
AAGTGGGAAC AGAAGCAGGT GCAGATCAAG ACCCTGGAGG GCGAGTTCTC GGTCACCATG 
TGGTCTTCAG ATGAAAAAAA AGATATTGAC CATGAAACAG TGGTTGAAGA GCAGATCATT 
GGGGAGAACT CACCTCCTGA TTATTCTGAA TATATGACAG GCAAGAAACT CCCTCCTGGA 
GGGATACCTG GCATTGACCT CTCAGACCCC AAGCAACTGG CAGAATTTGC CAGAATGAAG 
CCAAGAAAAA TTAAAGAAGA TGATGCTCCA AGAACAATAG CTTGCCCTCA TAAACTGTCT 
AGTGGTGACC TGTGCTGTCC TTCTGTCATG CTGTCTCACC ATTTCCCCAC TCACTCCATC 
ATGGGCAACA ACTGCAGAAG AAATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240599.1
CDS	139..1128
Translation	MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEDDDEDDEDGGGGDHGGGGGHGHAGHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGSSSGGGRVKKGGGKKSGKKSYLGSGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKLSSGDLCCPSVMLSHHFPTHSIMGNNCRRNC

Target ORF information:

RefSeq Version	XM_006240537.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus YY1 transcription factor (Yy1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240537.3

ORF Insert Sequence:

ATGGCCTCGG GCGACACCCT CTACATTGCC ACGGACGGCT CGGAGATGCC AGCCGAGATC 
GTGGAACTGC ATGAGATTGA GGTGGAGACC ATCCCGGTGG AGACTATCGA GACCACGGTG 
GTGGGCGAGG AGGAGGACGA CGACGAAGAC GACGAGGATG GTGGCGGCGG AGACCACGGT 
GGCGGGGGCG GCCACGGGCA CGCTGGCCAC CACCATCACC ACCACCACCA CCACCACCCG 
CCCATGATCG CGCTGCAGCC GCTGGTCACC GACGACCCGA CCCAAGTGCA CCACCACCAA 
GAGGTGATTC TGGTGCAGAC GCGCGAGGAG GTAGTGGGTG GCGACGACTC GGACGGGCTG 
CGCGCCGAGG ACGGGTTCGA GGACCAGATC CTCATTCCGG TACCCGCGCC GGCCGGCGGA 
GACGACGACT ACATCGAGCA GACGCTGGTC ACCGTGGCGG CGGCCGGCAA GAGCGGTGGC 
GGGTCTTCGT CGGGCGGCGG CCGCGTTAAG AAGGGCGGCG GCAAGAAGAG TGGCAAGAAG 
AGTTACCTGG GCAGCGGGGC CGGCGCGGCG GGCGGTGGCG GCGCCGACCC GGGTAATAAG 
AAGTGGGAAC AGAAGCAGGT GCAGATCAAG ACCCTGGAGG GCGAGTTCTC GGTCACCATG 
TGGTCTTCAG ATGAAAAAAA AGATATTGAC CATGAAACAG TGGTTGAAGA GCAGATCATT 
GGGGAGAACT CACCTCCTGA TTATTCTGAA TATATGACAG GCAAGAAACT CCCTCCTGGA 
GGGATACCTG GCATTGACCT CTCAGACCCC AAGCAACTGG CAGAATTTGC CAGAATGAAG 
CCAAGAAAAA TTAAAGAAGA TGATGCTCCA AGAACAATAG CTTGCCCTCA TAAACTGTCT 
AGTGGTGACC TGTGCTGTCC TTCTGTCATG CTGTCTCACC ATTTCCCCAC TCACTCCATC 
ATGGGCAACA ACTGCAGAAG AAATTGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An integrated transcriptomics-guided genome-wide promoter analysis and next-generation proteomics approach to mine factor(s) regulating cellular differentiation.
DNA research : an international journal for rapid publication of reports on genes and genomes24(2)143-157(2017 Apr)
Mandal K,Bader SL,Kumar P,Malakar D,Campbell DS,Pradhan BS,Sarkar RK,Wadhwa N,Sensharma S,Jain V,Moritz RL,Majumdar SS

Transcriptional Regulation of the Astrocytic Excitatory Amino Acid Transporter 1 (EAAT1) via NF-?B and Yin Yang 1 (YY1).
The Journal of biological chemistry290(39)23725-37(2015 Sep)
Karki P,Kim C,Smith K,Son DS,Aschner M,Lee E

Elevated microRNA-155 promotes foam cell formation by targeting HBP1 in atherogenesis.
Cardiovascular research103(1)100-10(2014 Jul)
Tian FJ,An LN,Wang GK,Zhu JQ,Li Q,Zhang YY,Zeng A,Zou J,Zhu RF,Han XS,Shen N,Yang HT,Zhao XX,Huang S,Qin YW,Jing Q

Yin Yang 1 is a repressor of glutamate transporter EAAT2, and it mediates manganese-induced decrease of EAAT2 expression in astrocytes.
Molecular and cellular biology34(7)1280-9(2014 Apr)
Karki P,Webb A,Smith K,Johnson J Jr,Lee K,Son DS,Aschner M,Lee E

The primary microRNA-208b interacts with Polycomb-group protein, Ezh2, to regulate gene expression in the heart.
Nucleic acids research42(2)790-803(2014 Jan)
Mathiyalagan P,Okabe J,Chang L,Su Y,Du XJ,El-Osta A

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Yy1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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