Gnas cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnas
Entrez Gene ID	24896
Full Name	GNAS complex locus
Synonyms	ALEX,G-alpha-8,Gnas1,Gnpas,Nesp55,SCG6
General protein information	Preferred Names GNAS complex locus Names ALEX GNAS SCG6 (secretogranin VI) GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus GNAS complex locus XLas-like adenylate cyclase-stimulating G alpha protein guanine nucleotide binding protein alpha s guanine nucleotide-binding protein G-s, alpha subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q43
Summary	This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001359867.1	NP_001346796.1	GNAS isoform b
NM_001024823.4	NP_001019994.4	ALEX isoform Alex
NM_019132.1	NP_062005.1	GNAS isoform GNASL
NM_021845.5	NP_068617.6	GNAS isoform XLas
NM_001159653.1	NP_001153125.1	SCG6 (secretogranin VI) isoform SCG6 precursor
NM_019132.2	NP_062005.1	GNAS isoform GNASL
NM_001159656.1	NP_001153128.1	SCG6 (secretogranin VI) isoform SCG6 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GNAS	NP_001003263.1
Rattus norvegicus (Norway rat)	Gnas	NP_068617.6
Caenorhabditis elegans (roundworm)	gsa-1	NP_490817.3
Xenopus tropicalis (tropical clawed frog)	gnas	NP_998855.1
Pan troglodytes (chimpanzee)	GNAS	XP_001138016.3
Drosophila melanogaster (fruit fly)	G-salpha60A	NP_726388.1
Anopheles gambiae (African malaria mosquito)	GNAS_ANOGA	XP_320433.1
Bos taurus (cattle)	GNAS	NP_851364.1
Mus musculus (house mouse)	Gnas	NP_034439.2
Gallus gallus (chicken)	GNAS	XP_417485.3
Homo sapiens (human)	GNAS	NP_536350.2
Danio rerio (zebrafish)	gnas	XP_005172181.1
	GNAS	XP_001083687.2

Gene Ontology
Bibliography

Related articles in PubMed

TGR5 contributes to hepatic cystogenesis in rodents with polycystic liver diseases through cyclic adenosine monophosphate/G?s signaling.
Masyuk TV, Masyuk AI, Lorenzo Pisarello M, Howard BN, Huang BQ, Lee PY, Fung X, Sergienko E, Ardecky RJ, Chung TDY, Pinkerton AB, LaRusso NF
Hepatology (Baltimore, Md.)66(4)1197-1218(2017 10)

The Characteristics of Hepatic Gs?-cAMP Axis in HSHF Diet-Fed Obese Insulin Resistance Rats and Genetic Diabetic Mice.
Xue N, Wei C, Zhang L, Liu H, Wang X, Wang L
Biological & pharmaceutical bulletin40(6)774-781(2017 Jun)

Antidepressants Accumulate in Lipid Rafts Independent of Monoamine Transporters to Modulate Redistribution of the G Protein, G?s.
Erb SJ, Schappi JM, Rasenick MM
The Journal of biological chemistry291(38)19725-19733(2016 09)

Activation of microtubule dynamics increases neuronal growth via the nerve growth factor (NGF)- and G?s-mediated signaling pathways.
Sarma T, Koutsouris A, Yu JZ, Krbanjevic A, Hope TJ, Rasenick MM
The Journal of biological chemistry290(16)10045-56(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Translocation of Galphas, which shows delayed onset after chronic antidepressant treatment of rats or of C6 glioma cells, tracks with the delayed onset of therapeutic action of antidepressants
Title: Antidepressants Accumulate in Lipid Rafts Independent of Monoamine Transporters to Modulate Redistribution of the G Protein, G?s.

Activated Galpha s translocates from the plasma membrane and, through interaction with tubulin/microtubules in the cytosol, is important for neurite formation, development, and outgrowth.
Title: Activation of microtubule dynamics increases neuronal growth via the nerve growth factor (NGF)- and G?s-mediated signaling pathways.

Results suggest that the suppression of Dopamine-2 (D2) receptor short (D2S)-regulated Gi3 inhibition of Gs protein may be one of the mechanisms controlling estradiol (E(2)-activated prolactin (PRL) synthesis and cell proliferation in lactotropes.
Title: Estrogen inhibits D2S receptor-regulated Gi3 and Gs protein interactions to stimulate prolactin production and cell proliferation in lactotropic cells.

activated XLalphas and Galphas traffic differently, and this may form the basis for the differences in their cellular actions
Title: Extra-long G?s variant XL?s protein escapes activation-induced subcellular redistribution and is able to provide sustained signaling.

Data show that the interdomain opening is coupled to receptor binding via the C-terminal helix of G(alpha), the extension of which is a high-affinity receptor binding element.
Title: Interaction of a G protein with an activated receptor opens the interdomain interface in the alpha subunit.

The following Gnas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42329	NM_019132.2 Latest version!	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa63308	NM_001359867.1 Latest version!	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa42770	NM_001024823.4 Latest version!	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
ORa42778	NM_021845.5 Latest version!	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
ORa42795	NM_001159653.1 Latest version!	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa42795	NM_001159656.1 Latest version!	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
ORa42329	NM_019132.1	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnas ( NM_019132.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062005.1 Rattus norvegicus GNAS complex locus (Gnas), transcript variant 3, mRNA.

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CloneID	ORa42329
Clone ID Related Accession (Same CDS sequence)	NM_019132.1 , NM_019132.2
Accession Version	NM_019132.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Rattus norvegicus(Norway rat)
Product	GNAS isoform GNASL
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M17525.1. On Dec 17, 2003 this sequence version replaced XM_342596.1. Transcript Variant: This variant (3) is biallelically expressed and encodes isoform GNASL, also known as gnas1, a form of the G-protein alpha subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M17525.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTAC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACC AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCCGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCAAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTTCGT 
GCCTGCTACG AGCGCTCCAA CGAGTACCAG CTGATCGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGCGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAA CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT CCGGGAGGAC 
AACCAGACCA ACCGTCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGTA CCATCTCTGT GATCCTCTTC CTCAACAAGC AAGATCTGCT TGCTGAGAAG 
GTCCTCGCTG GGAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCCGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATCCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGACGTCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTTCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062005.1
CDS	183..1367
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_019132.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019132.1

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTAC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACC AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCCGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCAAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTTCGT 
GCCTGCTACG AGCGCTCCAA CGAGTACCAG CTGATCGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGCGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAA CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT CCGGGAGGAC 
AACCAGACCA ACCGTCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGTA CCATCTCTGT GATCCTCTTC CTCAACAAGC AAGATCTGCT TGCTGAGAAG 
GTCCTCGCTG GGAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCCGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATCCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGACGTCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTTCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_019132.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062005.1 Rattus norvegicus GNAS complex locus (Gnas), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa42329
Clone ID Related Accession (Same CDS sequence)	NM_019132.1 , NM_019132.2
Accession Version	NM_019132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Rattus norvegicus(Norway rat)
Product	GNAS isoform GNASL
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M12673.1, M17525.1 and FQ214546.1. On Jan 18, 2018 this sequence version replaced NM_019132.1. Transcript Variant: This variant (3) is biallelically expressed and encodes isoform GNASL, also known as gnas1, a form of the G-protein alpha subunit. Sequence Note: This RefSeq record was created from transcript data from different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 23839232 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTAC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACC AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCCGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCAAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTTCGT 
GCCTGCTACG AGCGCTCCAA CGAGTACCAG CTGATCGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGCGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAA CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT CCGGGAGGAC 
AACCAGACCA ACCGTCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGTA CCATCTCTGT GATCCTCTTC CTCAACAAGC AAGATCTGCT TGCTGAGAAG 
GTCCTCGCTG GGAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCCGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATCCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGACGTCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTTCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062005.1
CDS	247..1431
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_019132.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019132.2

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTAC ATGTTAATGG GTTTAACGGA GAGGGCGGCG AAGAGGACCC GCAGGCTGCA 
AGGAGCAACA GCGATGGTGA GAAGGCCACC AAAGTGCAGG ACATCAAAAA CAACCTGAAG 
GAGGCCATTG AAACCATTGT GGCCGCCATG AGCAACCTGG TGCCCCCCGT GGAGCTGGCC 
AACCCTGAGA ACCAGTTCAG AGTGGACTAC ATTCTGAGCG TGATGAACGT GCCAAACTTT 
GACTTCCCAC CTGAATTCTA TGAGCATGCC AAGGCTCTGT GGGAGGATGA GGGAGTTCGT 
GCCTGCTACG AGCGCTCCAA CGAGTACCAG CTGATCGACT GTGCCCAGTA CTTCCTGGAC 
AAGATTGATG TGATCAAGCA GGCCGACTAC GTGCCAAGTG ACCAGGACCT GCTTCGCTGC 
CGCGTCCTGA CCTCTGGAAT CTTTGAGACC AAGTTCCAGG TGGACAAAGT CAACTTCCAC 
ATGTTCGATG TGGGCGGCCA GCGCGATGAA CGCCGCAAGT GGATCCAGTG CTTCAATGAT 
GTGACTGCCA TCATCTTCGT GGTGGCCAGC AGCAGCTACA ACATGGTCAT CCGGGAGGAC 
AACCAGACCA ACCGTCTGCA GGAGGCTCTG AACCTCTTCA AGAGCATCTG GAACAACAGA 
TGGCTGCGTA CCATCTCTGT GATCCTCTTC CTCAACAAGC AAGATCTGCT TGCTGAGAAG 
GTCCTCGCTG GGAAATCGAA GATTGAGGAC TACTTTCCAG AGTTCGCTCG CTACACCACT 
CCTGAGGATG CGACTCCCGA GCCCGGAGAG GACCCACGCG TGACCCGGGC CAAGTACTTC 
ATCCGGGATG AGTTTCTGAG AATCAGCACT GCTAGTGGAG ATGGACGTCA CTACTGCTAC 
CCTCACTTTA CCTGCGCCGT GGACACTGAG AACATCCGCC GTGTCTTCAA CGACTGCCGT 
GACATCATCC AGCGCATGCA TCTTCGCCAA TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001359867.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001346796.1 Rattus norvegicus GNAS complex locus (Gnas), transcript variant 6, mRNA.

Price & Availability↑

CloneID	ORa63308
Clone ID Related Accession (Same CDS sequence)	NM_001359867.1
Accession Version	NM_001359867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Rattus norvegicus(Norway rat)
Product	GNAS isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M12673.1, FQ215882.1 and FQ214546.1. Transcript Variant: This variant (6) lacks an in-frame exon compared to variant 3 and encodes an alternative G-protein alpha subunit (isoform b). Sequence Note: This RefSeq record was created from transcript data from different strains because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## imprinted gene :: PMID: 23839232 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTAC ATGTTAATGG GTTTAACGGA GATGAGAAGG CCACCAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCCGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCAA ACTTTGACTT CCCACCTGAA TTCTGTGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TTCGTGCCTG CTACGAGCGC TCCAACGAGT ACCAGCTGAT CGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGCGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAACGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TCCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATCCGGG AGGACAACCA GACCAACCGT CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGTACCATC TCTGTGATCC TGTTCCTCAA CAAGCAAGAT 
CTGCTTGCTG AGAAGGTCCT CGTTGGGAAA TCGAAGATTG AGGACTACTT TCCAGAGTTG 
GCTCGGTACA CCACTCCTGG GGATGGGACT CCCGAGCCGG GAGTGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATCCG AGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGA 
CGTCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTTC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346796.1
CDS	247..1386
Translation	MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGDEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFCEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIISVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLVGKSKIEDYFPELARYTTPGDGTPEPGVDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_001359867.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359867.1

ORF Insert Sequence:

ATGGGCTGCC TCGGCAACAG TAAGACCGAG GACCAGCGCA ACGAGGAGAA GGCGCAGCGC 
GAGGCCAACA AAAAGATCGA GAAGCAGCTG CAGAAGGACA AGCAGGTCTA CCGGGCCACG 
CACCGCCTGC TGCTGCTGGG TGCTGGAGAG TCTGGCAAAA GCACCATTGT GAAGCAGATG 
AGGATCCTAC ATGTTAATGG GTTTAACGGA GATGAGAAGG CCACCAAAGT GCAGGACATC 
AAAAACAACC TGAAGGAGGC CATTGAAACC ATTGTGGCCG CCATGAGCAA CCTGGTGCCC 
CCCGTGGAGC TGGCCAACCC TGAGAACCAG TTCAGAGTGG ACTACATTCT GAGCGTGATG 
AACGTGCCAA ACTTTGACTT CCCACCTGAA TTCTGTGAGC ATGCCAAGGC TCTGTGGGAG 
GATGAGGGAG TTCGTGCCTG CTACGAGCGC TCCAACGAGT ACCAGCTGAT CGACTGTGCC 
CAGTACTTCC TGGACAAGAT TGATGTGATC AAGCAGGCCG ACTACGTGCC AAGTGACCAG 
GACCTGCTTC GCTGCCGCGT CCTGACCTCT GGAATCTTTG AGACCAAGTT CCAGGTGGAC 
AAAGTCAACT TCCACATGTT CGATGTGGGC GGCCAGCGCG ATGAACGCCG CAAGTGGATC 
CAGTGCTTCA ATGATGTGAC TGCCATCATC TCCGTGGTGG CCAGCAGCAG CTACAACATG 
GTCATCCGGG AGGACAACCA GACCAACCGT CTGCAGGAGG CTCTGAACCT CTTCAAGAGC 
ATCTGGAACA ACAGATGGCT GCGTACCATC TCTGTGATCC TGTTCCTCAA CAAGCAAGAT 
CTGCTTGCTG AGAAGGTCCT CGTTGGGAAA TCGAAGATTG AGGACTACTT TCCAGAGTTG 
GCTCGGTACA CCACTCCTGG GGATGGGACT CCCGAGCCGG GAGTGGACCC ACGCGTGACC 
CGGGCCAAGT ACTTCATCCG AGATGAGTTT CTGAGAATCA GCACTGCTAG TGGAGATGGA 
CGTCACTACT GCTACCCTCA CTTTACCTGC GCCGTGGACA CTGAGAACAT CCGCCGTGTC 
TTCAACGACT GCCGTGACAT CATCCAGCGC ATGCATCTTC GCCAATACGA GCTGCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001024823.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019994.4 Rattus norvegicus GNAS complex locus (Gnas), transcript variant 1, mRNA.

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CloneID	ORa42770
Clone ID Related Accession (Same CDS sequence)	NM_001024823.4
Accession Version	NM_001024823.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	ALEX isoform Alex
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AAHX01026999.1, X84047.1 and BC168156.1. On Jul 15, 2009 this sequence version replaced NM_001024823.3. Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, as compared to variant 3. This variant includes two overlapping open reading frames encoding XLas and Alex, respectively. This RefSeq represents Alex, also known as alexX, which has no similarity to other isoforms encoded by this gene. It interacts with XLas, and their interaction is essential for G-protein signaling in neuroendocrine cells. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMN01906348 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 23839232 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCAT CCCCGACGAG ACTGACAGTG AGGTCTGTGG ACCCCCTGAA GACTCCAAAT 
CTGACATCCA GAGCCCCAGT CAGGCCTTCG AGGAAGTCCA AGTGGGCGGA GACTACAGCC 
CACCTCCAGA GGAAGCCATG CCATTCGAGA TCCAACAGCC CAGCCTGGGA GATTTCTGGC 
CCACCCTGGA GCAGCCTGGA CCATCTGGGA CCCCATCAGG CATCAAAGCC TTCAACCCAG 
CGATTTTGGA GCCCGGGACC CCCACTGGTG CACACCCAGG CCTGGGAGCC TATAGCCCCC 
CACCAGAAGA AGCTATGCCA TTTGAGTTCA ATGAGCCTGC CCAGGAAGAC CGTTGCCAGC 
CTCCCTTGCA AGTCCCAGAC CTTGCGCCAG GGGGTCCGGA AGCATGGGTC TCCAGAGCTC 
TTCCCGCGGA GCCCGGGAAC CTCGGATTTG AAAACACTGG CTTCCGAGAA GACTACAGCC 
CTCCACCTGA AGAATCTGTG CCATTTCAGC TCGATGGAGA AGAATTCGGG GGCGATAGCC 
CACCCCCAGG ACTCCCGCGA GTCACGCCAC AAATCGGCAT TGGCGGCGAG TTCCCGACAG 
TCGCGGTCCC GAGTACGCTC TGCCTCGCTC CCGCCGCGAA CGCGCCTCCC CTCTGGGTCC 
AGGGCGCCAT TGGCAGACCA TTCCGCGAGG CTGTCAGATC TCCTAACTTC GCATACGACA 
TTTCCCCAAT GGAGATCACC AGACCCCTGC TTGAGATTGG CAGAGCCTCC ACTGGGGTCG 
ACGACGACAC CGCTGTCAAT ATGGACAGCC CCCCAATCGC AAGTGATGGC CCGCCCATCG 
AAGTCTCGGG AGCCCCAGTT AAGAGCGAGC ACGCAAAGAG ACCCCCACTT GAGCGACAAG 
CAGCCGAGAC AGGAAACAGC CCTATCAGCA GCACCACTGC AGAGGAGGCA AAAGTCCCCT 
CCCTCGAGCG AGGAGAAGGA TCCCCCACCC AACCTGAAAC AGTGCATATC AAGCCAGCTC 
CTGTTGCGGA GTCCGGAACG GACTCTTCCA AAGCCGATCC GGACTCAGCT ACACACGCAG 
TTCTTCAGAT CGGTCCTGAG GAAGTCGGAG GAGTCCCAAC CATGCCCACC GATCTTCCGC 
CTGCTTCTGA AGATGCGGGC CCAGATGTCC GGGCAGAACC AGACGGAGGG ACAGCCCCAG 
CCACCCCTGC CGAGTCCGAA GACAACAGAG AACCAGCCGC CGCCGCCGCC GCCGAGCCAG 
CCGCCGAGCC AGCCGCTGAG CCAGCCGCCG AGCCAGCCGC CGAGCCAGCC GCCGAGCCAG 
CTGCCGAGGC AGTCCCTGAC ACCGAAGCCG AGTCTGCCTC CGGGGCAGTC CCCGACACCC 
AAGAGGAGCC CGCAGCCGCG GCAGCCTCTG CCACGCCGGC GGAGCCTGCC GCCCGGGCAG 
CCCCCGTCAC CCCTACGGAG CCCGCTACCC GGGCTGTCCC TTCTGCCAGA GCCCATCCAG 
CCGCCGGGGC TGTCCCTGGA GCCTCAGCGA TGTCAGCCGC TGCTAGGGCA GCCGCCGCTA 
GAGCAGCCTA TGCAGGTCCT CTGGTCTGGG GAGCCAGGTC ACTCTCGGCT ACTCCAGCCG 
CTCGGGCATC CCTCCCTGCC CGCGCAGCAG CTGCCGCCCG AGCAGCCTCT GCTGCCCGCG 
CAGTCGCTGC CGGCCGGTCA GCCTCTGCCG CCCCAAGCAG GGCCCATCTT AGACCCCCCA 
GCCCGGAGAT CCAGGTTGCT GACCCGCCTA CTCCGCGGCC TGCTCCGCGG CCGAGTGCCT 
GGCCTGACAA ATACGAACGT GGCCGAAGCT GCTGCAGGTA TGAGGCTGCG TCCGGCATCT 
GCGAGATCGA GTCCTCCAGC GATGAGTCGG AAGAAGGGGC CACTGGCTGC TTCCAGTGGC 
TTCTGCGGCG AAACCGCCGC CCTGGCCAGC CCCGGAGCCA CACAGTCGGG AGCAACCCGG 
TCCGCAACTT CTTCGCCCGA GCCTTCGGAA GCTGCTTCGG TCTATCTGAG TGTACCCGAT 
CACGATCCCT CAGCCCCGGG AAGGCCAAGG ATCCTATGGA AGAGAGGCGC AAACAGATGC 
GCAAAGAAGC CATGGAGATG CGAGAGCAGA AGCGCGCAGA TAAGAAACGC AGCAAGCTCA 
TCGACAAGCA ACTGGAGGAG GAGAAGATGG ACTACATGTG TACACACCGC CTGCTGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019994.4
CDS	170..2392
Translation	MSPSPTRLTVRSVDPLKTPNLTSRAPVRPSRKSKWAETTAHLQRKPCHSRSNSPAWEISGPPWSSLDHLGPHQASKPSTQRFWSPGPPLVHTQAWEPIAPHQKKLCHLSSMSLPRKTVASLPCKSQTLRQGVRKHGSPELFPRSPGTSDLKTLASEKTTALHLKNLCHFSSMEKNSGAIAHPQDSRESRHKSALAASSRQSRSRVRSASLPPRTRLPSGSRAPLADHSARLSDLLTSHTTFPQWRSPDPCLRLAEPPLGSTTTPLSIWTAPQSQVMARPSKSREPQLRASTQRDPHLSDKQPRQETALSAAPLQRRQKSPPSSEEKDPPPNLKQCISSQLLLRSPERTLPKPIRTQLHTQFFRSVLRKSEESQPCPPIFRLLLKMRAQMSGQNQTEGQPQPPLPSPKTTENQPPPPPPSQPPSQPLSQPPSQPPSQPPSQLPRQSLTPKPSLPPGQSPTPKRSPQPRQPLPRRRSLPPGQPPSPLRSPLPGLSLLPEPIQPPGLSLEPQRCQPLLGQPPLEQPMQVLWSGEPGHSRLLQPLGHPSLPAQQLPPEQPLLPAQSLPAGQPLPPQAGPILDPPARRSRLLTRLLRGLLRGRVPGLTNTNVAEAAAGMRLRPASARSSPPAMSRKKGPLAASSGFCGETAALASPGATQSGATRSATSSPEPSEAASVYLSVPDHDPSAPGRPRILWKRGANRCAKKPWRCESRSAQIRNAASSSTSNWRRRRWTTCVHTACCF

Target ORF information:

RefSeq Version	NM_001024823.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024823.4

ORF Insert Sequence:

ATGAGCCCAT CCCCGACGAG ACTGACAGTG AGGTCTGTGG ACCCCCTGAA GACTCCAAAT 
CTGACATCCA GAGCCCCAGT CAGGCCTTCG AGGAAGTCCA AGTGGGCGGA GACTACAGCC 
CACCTCCAGA GGAAGCCATG CCATTCGAGA TCCAACAGCC CAGCCTGGGA GATTTCTGGC 
CCACCCTGGA GCAGCCTGGA CCATCTGGGA CCCCATCAGG CATCAAAGCC TTCAACCCAG 
CGATTTTGGA GCCCGGGACC CCCACTGGTG CACACCCAGG CCTGGGAGCC TATAGCCCCC 
CACCAGAAGA AGCTATGCCA TTTGAGTTCA ATGAGCCTGC CCAGGAAGAC CGTTGCCAGC 
CTCCCTTGCA AGTCCCAGAC CTTGCGCCAG GGGGTCCGGA AGCATGGGTC TCCAGAGCTC 
TTCCCGCGGA GCCCGGGAAC CTCGGATTTG AAAACACTGG CTTCCGAGAA GACTACAGCC 
CTCCACCTGA AGAATCTGTG CCATTTCAGC TCGATGGAGA AGAATTCGGG GGCGATAGCC 
CACCCCCAGG ACTCCCGCGA GTCACGCCAC AAATCGGCAT TGGCGGCGAG TTCCCGACAG 
TCGCGGTCCC GAGTACGCTC TGCCTCGCTC CCGCCGCGAA CGCGCCTCCC CTCTGGGTCC 
AGGGCGCCAT TGGCAGACCA TTCCGCGAGG CTGTCAGATC TCCTAACTTC GCATACGACA 
TTTCCCCAAT GGAGATCACC AGACCCCTGC TTGAGATTGG CAGAGCCTCC ACTGGGGTCG 
ACGACGACAC CGCTGTCAAT ATGGACAGCC CCCCAATCGC AAGTGATGGC CCGCCCATCG 
AAGTCTCGGG AGCCCCAGTT AAGAGCGAGC ACGCAAAGAG ACCCCCACTT GAGCGACAAG 
CAGCCGAGAC AGGAAACAGC CCTATCAGCA GCACCACTGC AGAGGAGGCA AAAGTCCCCT 
CCCTCGAGCG AGGAGAAGGA TCCCCCACCC AACCTGAAAC AGTGCATATC AAGCCAGCTC 
CTGTTGCGGA GTCCGGAACG GACTCTTCCA AAGCCGATCC GGACTCAGCT ACACACGCAG 
TTCTTCAGAT CGGTCCTGAG GAAGTCGGAG GAGTCCCAAC CATGCCCACC GATCTTCCGC 
CTGCTTCTGA AGATGCGGGC CCAGATGTCC GGGCAGAACC AGACGGAGGG ACAGCCCCAG 
CCACCCCTGC CGAGTCCGAA GACAACAGAG AACCAGCCGC CGCCGCCGCC GCCGAGCCAG 
CCGCCGAGCC AGCCGCTGAG CCAGCCGCCG AGCCAGCCGC CGAGCCAGCC GCCGAGCCAG 
CTGCCGAGGC AGTCCCTGAC ACCGAAGCCG AGTCTGCCTC CGGGGCAGTC CCCGACACCC 
AAGAGGAGCC CGCAGCCGCG GCAGCCTCTG CCACGCCGGC GGAGCCTGCC GCCCGGGCAG 
CCCCCGTCAC CCCTACGGAG CCCGCTACCC GGGCTGTCCC TTCTGCCAGA GCCCATCCAG 
CCGCCGGGGC TGTCCCTGGA GCCTCAGCGA TGTCAGCCGC TGCTAGGGCA GCCGCCGCTA 
GAGCAGCCTA TGCAGGTCCT CTGGTCTGGG GAGCCAGGTC ACTCTCGGCT ACTCCAGCCG 
CTCGGGCATC CCTCCCTGCC CGCGCAGCAG CTGCCGCCCG AGCAGCCTCT GCTGCCCGCG 
CAGTCGCTGC CGGCCGGTCA GCCTCTGCCG CCCCAAGCAG GGCCCATCTT AGACCCCCCA 
GCCCGGAGAT CCAGGTTGCT GACCCGCCTA CTCCGCGGCC TGCTCCGCGG CCGAGTGCCT 
GGCCTGACAA ATACGAACGT GGCCGAAGCT GCTGCAGGTA TGAGGCTGCG TCCGGCATCT 
GCGAGATCGA GTCCTCCAGC GATGAGTCGG AAGAAGGGGC CACTGGCTGC TTCCAGTGGC 
TTCTGCGGCG AAACCGCCGC CCTGGCCAGC CCCGGAGCCA CACAGTCGGG AGCAACCCGG 
TCCGCAACTT CTTCGCCCGA GCCTTCGGAA GCTGCTTCGG TCTATCTGAG TGTACCCGAT 
CACGATCCCT CAGCCCCGGG AAGGCCAAGG ATCCTATGGA AGAGAGGCGC AAACAGATGC 
GCAAAGAAGC CATGGAGATG CGAGAGCAGA AGCGCGCAGA TAAGAAACGC AGCAAGCTCA 
TCGACAAGCA ACTGGAGGAG GAGAAGATGG ACTACATGTG TACACACCGC CTGCTGCTTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_021845.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_068617.6 Rattus norvegicus GNAS complex locus (Gnas), transcript variant 1, mRNA.

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CloneID	ORa42778
Clone ID Related Accession (Same CDS sequence)	NM_021845.5
Accession Version	NM_021845.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Rattus norvegicus(Norway rat)
Product	GNAS isoform XLas
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AAHX01026999.1, X84047.1 and BC168156.1. On Jul 15, 2009 this sequence version replaced NM_021845.4. Transcript Variant: This variant (1, also known as GNASXL) is paternally expressed. It includes an alternate 5' exon, as compared to variant 3. This variant includes two overlapping open reading frames encoding XLas and Alex, respectively. This RefSeq represents XLas, also known as alpha s XXL, which is a neuroendocrine-specific G-protein alpha subunit; it has a distinct and much longer N-terminus, as compared to isoform GNASL. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMN01906348 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 23839232 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCATGC TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG ACCAGCCCGA GCAAGAACCT TTGGAAGCCC AAGGGGCCGC TGCCCCCGGT 
GCTGGGGTTG GCCCAGCCGA AGAAATGGAG ACCGAACCGT CTAACAATGA GCCCATCCCC 
GACGAGACTG ACAGTGAGGT CTGTGGACCC CCTGAAGACT CCAAATCTGA CATCCAGAGC 
CCCAGTCAGG CCTTCGAGGA AGTCCAAGTG GGCGGAGACT ACAGCCCACC TCCAGAGGAA 
GCCATGCCAT TCGAGATCCA ACAGCCCAGC CTGGGAGATT TCTGGCCCAC CCTGGAGCAG 
CCTGGACCAT CTGGGACCCC ATCAGGCATC AAAGCCTTCA ACCCAGCGAT TTTGGAGCCC 
GGGACCCCCA CTGGTGCACA CCCAGGCCTG GGAGCCTATA GCCCCCCACC AGAAGAAGCT 
ATGCCATTTG AGTTCAATGA GCCTGCCCAG GAAGACCGTT GCCAGCCTCC CTTGCAAGTC 
CCAGACCTTG CGCCAGGGGG TCCGGAAGCA TGGGTCTCCA GAGCTCTTCC CGCGGAGCCC 
GGGAACCTCG GATTTGAAAA CACTGGCTTC CGAGAAGACT ACAGCCCTCC ACCTGAAGAA 
TCTGTGCCAT TTCAGCTCGA TGGAGAAGAA TTCGGGGGCG ATAGCCCACC CCCAGGACTC 
CCGCGAGTCA CGCCACAAAT CGGCATTGGC GGCGAGTTCC CGACAGTCGC GGTCCCGAGT 
ACGCTCTGCC TCGCTCCCGC CGCGAACGCG CCTCCCCTCT GGGTCCAGGG CGCCATTGGC 
AGACCATTCC GCGAGGCTGT CAGATCTCCT AACTTCGCAT ACGACATTTC CCCAATGGAG 
ATCACCAGAC CCCTGCTTGA GATTGGCAGA GCCTCCACTG GGGTCGACGA CGACACCGCT 
GTCAATATGG ACAGCCCCCC AATCGCAAGT GATGGCCCGC CCATCGAAGT CTCGGGAGCC 
CCAGTTAAGA GCGAGCACGC AAAGAGACCC CCACTTGAGC GACAAGCAGC CGAGACAGGA 
AACAGCCCTA TCAGCAGCAC CACTGCAGAG GAGGCAAAAG TCCCCTCCCT CGAGCGAGGA 
GAAGGATCCC CCACCCAACC TGAAACAGTG CATATCAAGC CAGCTCCTGT TGCGGAGTCC 
GGAACGGACT CTTCCAAAGC CGATCCGGAC TCAGCTACAC ACGCAGTTCT TCAGATCGGT 
CCTGAGGAAG TCGGAGGAGT CCCAACCATG CCCACCGATC TTCCGCCTGC TTCTGAAGAT 
GCGGGCCCAG ATGTCCGGGC AGAACCAGAC GGAGGGACAG CCCCAGCCAC CCCTGCCGAG 
TCCGAAGACA ACAGAGAACC AGCCGCCGCC GCCGCCGCCG AGCCAGCCGC CGAGCCAGCC 
GCTGAGCCAG CCGCCGAGCC AGCCGCCGAG CCAGCCGCCG AGCCAGCTGC CGAGGCAGTC 
CCTGACACCG AAGCCGAGTC TGCCTCCGGG GCAGTCCCCG ACACCCAAGA GGAGCCCGCA 
GCCGCGGCAG CCTCTGCCAC GCCGGCGGAG CCTGCCGCCC GGGCAGCCCC CGTCACCCCT 
ACGGAGCCCG CTACCCGGGC TGTCCCTTCT GCCAGAGCCC ATCCAGCCGC CGGGGCTGTC 
CCTGGAGCCT CAGCGATGTC AGCCGCTGCT AGGGCAGCCG CCGCTAGAGC AGCCTATGCA 
GGTCCTCTGG TCTGGGGAGC CAGGTCACTC TCGGCTACTC CAGCCGCTCG GGCATCCCTC 
CCTGCCCGCG CAGCAGCTGC CGCCCGAGCA GCCTCTGCTG CCCGCGCAGT CGCTGCCGGC 
CGGTCAGCCT CTGCCGCCCC AAGCAGGGCC CATCTTAGAC CCCCCAGCCC GGAGATCCAG 
GTTGCTGACC CGCCTACTCC GCGGCCTGCT CCGCGGCCGA GTGCCTGGCC TGACAAATAC 
GAACGTGGCC GAAGCTGCTG CAGGTATGAG GCTGCGTCCG GCATCTGCGA GATCGAGTCC 
TCCAGCGATG AGTCGGAAGA AGGGGCCACT GGCTGCTTCC AGTGGCTTCT GCGGCGAAAC 
CGCCGCCCTG GCCAGCCCCG GAGCCACACA GTCGGGAGCA ACCCGGTCCG CAACTTCTTC 
GCCCGAGCCT TCGGAAGCTG CTTCGGTCTA TCTGAGTGTA CCCGATCACG ATCCCTCAGC 
CCCGGGAAGG CCAAGGATCC TATGGAAGAG AGGCGCAAAC AGATGCGCAA AGAAGCCATG 
GAGATGCGAG AGCAGAAGCG CGCAGATAAG AAACGCAGCA AGCTCATCGA CAAGCAACTG 
GAGGAGGAGA AGATGGACTA CATGTGTACA CACCGCCTGC TGCTTCTAGG TGCTGGAGAG 
TCTGGCAAAA GCACCATTGT GAAGCAGATG AGGATCCTAC ATGTTAATGG GTTTAACGGA 
GAGGGCGGCG AAGAGGACCC GCAGGCTGCA AGGAGCAACA GCGATGGTGA GAAGGCCACC 
AAAGTGCAGG ACATCAAAAA CAACCTGAAG GAGGCCATTG AAACCATTGT GGCCGCCATG 
AGCAACCTGG TGCCCCCCGT GGAGCTGGCC AACCCTGAGA ACCAGTTCAG AGTGGACTAC 
ATTCTGAGCG TGATGAACGT GCCAAACTTT GACTTCCCAC CTGAATTCTA TGAGCATGCC 
AAGGCTCTGT GGGAGGATGA GGGAGTTCGT GCCTGCTACG AGCGCTCCAA CGAGTACCAG 
CTGATCGACT GTGCCCAGTA CTTCCTGGAC AAGATTGATG TGATCAAGCA GGCCGACTAC 
GTGCCAAGTG ACCAGGACCT GCTTCGCTGC CGCGTCCTGA CCTCTGGAAT CTTTGAGACC 
AAGTTCCAGG TGGACAAAGT CAACTTCCAC ATGTTCGATG TGGGCGGCCA GCGCGATGAA 
CGCCGCAAGT GGATCCAGTG CTTCAATGAT GTGACTGCCA TCATCTTCGT GGTGGCCAGC 
AGCAGCTACA ACATGGTCAT CCGGGAGGAC AACCAGACCA ACCGTCTGCA GGAGGCTCTG 
AACCTCTTCA AGAGCATCTG GAACAACAGA TGGCTGCGTA CCATCTCTGT GATCCTCTTC 
CTCAACAAGC AAGATCTGCT TGCTGAGAAG GTCCTCGCTG GGAAATCGAA GATTGAGGAC 
TACTTTCCAG AGTTCGCTCG CTACACCACT CCTGAGGATG CGACTCCCGA GCCCGGAGAG 
GACCCACGCG TGACCCGGGC CAAGTACTTC ATCCGGGATG AGTTTCTGAG AATCAGCACT 
GCTAGTGGAG ATGGACGTCA CTACTGCTAC CCTCACTTTA CCTGCGCCGT GGACACTGAG 
AACATCCGCC GTGTCTTCAA CGACTGCCGT GACATCATCC AGCGCATGCA TCTTCGCCAA 
TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068617.6
CDS	4..3438
Translation	MGMLNCLHGNNMSGQHDIPPEVGDQPEQEPLEAQGAAAPGAGVGPAEEMETEPSNNEPIPDETDSEVCGPPEDSKSDIQSPSQAFEEVQVGGDYSPPPEEAMPFEIQQPSLGDFWPTLEQPGPSGTPSGIKAFNPAILEPGTPTGAHPGLGAYSPPPEEAMPFEFNEPAQEDRCQPPLQVPDLAPGGPEAWVSRALPAEPGNLGFENTGFREDYSPPPEESVPFQLDGEEFGGDSPPPGLPRVTPQIGIGGEFPTVAVPSTLCLAPAANAPPLWVQGAIGRPFREAVRSPNFAYDISPMEITRPLLEIGRASTGVDDDTAVNMDSPPIASDGPPIEVSGAPVKSEHAKRPPLERQAAETGNSPISSTTAEEAKVPSLERGEGSPTQPETVHIKPAPVAESGTDSSKADPDSATHAVLQIGPEEVGGVPTMPTDLPPASEDAGPDVRAEPDGGTAPATPAESEDNREPAAAAAAEPAAEPAAEPAAEPAAEPAAEPAAEAVPDTEAESASGAVPDTQEEPAAAAASATPAEPAARAAPVTPTEPATRAVPSARAHPAAGAVPGASAMSAAARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRAHLRPPSPEIQVADPPTPRPAPRPSAWPDKYERGRSCCRYEAASGICEIESSSDESEEGATGCFQWLLRRNRRPGQPRSHTVGSNPVRNFFARAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAMEMREQKRADKKRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

Target ORF information:

RefSeq Version	NM_021845.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021845.5

ORF Insert Sequence:

ATGGGCATGC TCAACTGCCT CCACGGCAAT AATATGTCAG GACAACACGA TATCCCCCCT 
GAAGTCGGGG ACCAGCCCGA GCAAGAACCT TTGGAAGCCC AAGGGGCCGC TGCCCCCGGT 
GCTGGGGTTG GCCCAGCCGA AGAAATGGAG ACCGAACCGT CTAACAATGA GCCCATCCCC 
GACGAGACTG ACAGTGAGGT CTGTGGACCC CCTGAAGACT CCAAATCTGA CATCCAGAGC 
CCCAGTCAGG CCTTCGAGGA AGTCCAAGTG GGCGGAGACT ACAGCCCACC TCCAGAGGAA 
GCCATGCCAT TCGAGATCCA ACAGCCCAGC CTGGGAGATT TCTGGCCCAC CCTGGAGCAG 
CCTGGACCAT CTGGGACCCC ATCAGGCATC AAAGCCTTCA ACCCAGCGAT TTTGGAGCCC 
GGGACCCCCA CTGGTGCACA CCCAGGCCTG GGAGCCTATA GCCCCCCACC AGAAGAAGCT 
ATGCCATTTG AGTTCAATGA GCCTGCCCAG GAAGACCGTT GCCAGCCTCC CTTGCAAGTC 
CCAGACCTTG CGCCAGGGGG TCCGGAAGCA TGGGTCTCCA GAGCTCTTCC CGCGGAGCCC 
GGGAACCTCG GATTTGAAAA CACTGGCTTC CGAGAAGACT ACAGCCCTCC ACCTGAAGAA 
TCTGTGCCAT TTCAGCTCGA TGGAGAAGAA TTCGGGGGCG ATAGCCCACC CCCAGGACTC 
CCGCGAGTCA CGCCACAAAT CGGCATTGGC GGCGAGTTCC CGACAGTCGC GGTCCCGAGT 
ACGCTCTGCC TCGCTCCCGC CGCGAACGCG CCTCCCCTCT GGGTCCAGGG CGCCATTGGC 
AGACCATTCC GCGAGGCTGT CAGATCTCCT AACTTCGCAT ACGACATTTC CCCAATGGAG 
ATCACCAGAC CCCTGCTTGA GATTGGCAGA GCCTCCACTG GGGTCGACGA CGACACCGCT 
GTCAATATGG ACAGCCCCCC AATCGCAAGT GATGGCCCGC CCATCGAAGT CTCGGGAGCC 
CCAGTTAAGA GCGAGCACGC AAAGAGACCC CCACTTGAGC GACAAGCAGC CGAGACAGGA 
AACAGCCCTA TCAGCAGCAC CACTGCAGAG GAGGCAAAAG TCCCCTCCCT CGAGCGAGGA 
GAAGGATCCC CCACCCAACC TGAAACAGTG CATATCAAGC CAGCTCCTGT TGCGGAGTCC 
GGAACGGACT CTTCCAAAGC CGATCCGGAC TCAGCTACAC ACGCAGTTCT TCAGATCGGT 
CCTGAGGAAG TCGGAGGAGT CCCAACCATG CCCACCGATC TTCCGCCTGC TTCTGAAGAT 
GCGGGCCCAG ATGTCCGGGC AGAACCAGAC GGAGGGACAG CCCCAGCCAC CCCTGCCGAG 
TCCGAAGACA ACAGAGAACC AGCCGCCGCC GCCGCCGCCG AGCCAGCCGC CGAGCCAGCC 
GCTGAGCCAG CCGCCGAGCC AGCCGCCGAG CCAGCCGCCG AGCCAGCTGC CGAGGCAGTC 
CCTGACACCG AAGCCGAGTC TGCCTCCGGG GCAGTCCCCG ACACCCAAGA GGAGCCCGCA 
GCCGCGGCAG CCTCTGCCAC GCCGGCGGAG CCTGCCGCCC GGGCAGCCCC CGTCACCCCT 
ACGGAGCCCG CTACCCGGGC TGTCCCTTCT GCCAGAGCCC ATCCAGCCGC CGGGGCTGTC 
CCTGGAGCCT CAGCGATGTC AGCCGCTGCT AGGGCAGCCG CCGCTAGAGC AGCCTATGCA 
GGTCCTCTGG TCTGGGGAGC CAGGTCACTC TCGGCTACTC CAGCCGCTCG GGCATCCCTC 
CCTGCCCGCG CAGCAGCTGC CGCCCGAGCA GCCTCTGCTG CCCGCGCAGT CGCTGCCGGC 
CGGTCAGCCT CTGCCGCCCC AAGCAGGGCC CATCTTAGAC CCCCCAGCCC GGAGATCCAG 
GTTGCTGACC CGCCTACTCC GCGGCCTGCT CCGCGGCCGA GTGCCTGGCC TGACAAATAC 
GAACGTGGCC GAAGCTGCTG CAGGTATGAG GCTGCGTCCG GCATCTGCGA GATCGAGTCC 
TCCAGCGATG AGTCGGAAGA AGGGGCCACT GGCTGCTTCC AGTGGCTTCT GCGGCGAAAC 
CGCCGCCCTG GCCAGCCCCG GAGCCACACA GTCGGGAGCA ACCCGGTCCG CAACTTCTTC 
GCCCGAGCCT TCGGAAGCTG CTTCGGTCTA TCTGAGTGTA CCCGATCACG ATCCCTCAGC 
CCCGGGAAGG CCAAGGATCC TATGGAAGAG AGGCGCAAAC AGATGCGCAA AGAAGCCATG 
GAGATGCGAG AGCAGAAGCG CGCAGATAAG AAACGCAGCA AGCTCATCGA CAAGCAACTG 
GAGGAGGAGA AGATGGACTA CATGTGTACA CACCGCCTGC TGCTTCTAGG TGCTGGAGAG 
TCTGGCAAAA GCACCATTGT GAAGCAGATG AGGATCCTAC ATGTTAATGG GTTTAACGGA 
GAGGGCGGCG AAGAGGACCC GCAGGCTGCA AGGAGCAACA GCGATGGTGA GAAGGCCACC 
AAAGTGCAGG ACATCAAAAA CAACCTGAAG GAGGCCATTG AAACCATTGT GGCCGCCATG 
AGCAACCTGG TGCCCCCCGT GGAGCTGGCC AACCCTGAGA ACCAGTTCAG AGTGGACTAC 
ATTCTGAGCG TGATGAACGT GCCAAACTTT GACTTCCCAC CTGAATTCTA TGAGCATGCC 
AAGGCTCTGT GGGAGGATGA GGGAGTTCGT GCCTGCTACG AGCGCTCCAA CGAGTACCAG 
CTGATCGACT GTGCCCAGTA CTTCCTGGAC AAGATTGATG TGATCAAGCA GGCCGACTAC 
GTGCCAAGTG ACCAGGACCT GCTTCGCTGC CGCGTCCTGA CCTCTGGAAT CTTTGAGACC 
AAGTTCCAGG TGGACAAAGT CAACTTCCAC ATGTTCGATG TGGGCGGCCA GCGCGATGAA 
CGCCGCAAGT GGATCCAGTG CTTCAATGAT GTGACTGCCA TCATCTTCGT GGTGGCCAGC 
AGCAGCTACA ACATGGTCAT CCGGGAGGAC AACCAGACCA ACCGTCTGCA GGAGGCTCTG 
AACCTCTTCA AGAGCATCTG GAACAACAGA TGGCTGCGTA CCATCTCTGT GATCCTCTTC 
CTCAACAAGC AAGATCTGCT TGCTGAGAAG GTCCTCGCTG GGAAATCGAA GATTGAGGAC 
TACTTTCCAG AGTTCGCTCG CTACACCACT CCTGAGGATG CGACTCCCGA GCCCGGAGAG 
GACCCACGCG TGACCCGGGC CAAGTACTTC ATCCGGGATG AGTTTCTGAG AATCAGCACT 
GCTAGTGGAG ATGGACGTCA CTACTGCTAC CCTCACTTTA CCTGCGCCGT GGACACTGAG 
AACATCCGCC GTGTCTTCAA CGACTGCCGT GACATCATCC AGCGCATGCA TCTTCGCCAA 
TACGAGCTGC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001159653.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001153125.1 Rattus norvegicus GNAS complex locus (Gnas), transcript variant 4, mRNA.

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CloneID	ORa42795
Clone ID Related Accession (Same CDS sequence)	NM_001159653.1 , NM_001159656.1
Accession Version	NM_001159653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	SCG6 (secretogranin VI) isoform SCG6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB770483.1, AF105254.1 and BC168156.1. On Apr 30, 2009 this sequence version replaced XM_001076436.1. Transcript Variant: This variant (4) is maternally expressed. It has alternate 5' exons, as compared to variant 3. Variants 4 and 5 both encode neuroendocrine secretory protein 55 (NESP55), which localizes to large secretory vesicles of endocrine cells and neurons. The coding regions of variants 4 and 5 do not overlap the coding regions used by other transcripts; thus NESP55 has no similarity to isoforms of the G-protein alpha subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 23839232 NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATCGCA GGTCCCGGGC TCATCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCTACC GCTCTCCTCT GGCTCTCCTG CTCCATCGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGGGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCC 
GAGTCCTCTG AATCCGAATC TGATCACGAG CACGAGGAGG CTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCCAGTC CGAGACCGAA TTCGAGACCG AGCCTGAGAC CGAGCCTGAG 
ACCGCCCCTA CAACTGAGCC TGAGACCGAA CCAGAGGACG AGCGCGGCCC CCGGGGCGCC 
ACCTTCAACC AGTCACTCAC TCAGCGTCTG CACGCTCTGA AGTTGCAGAG CGCCGACGCC 
TCCCCGAGAC GTGCGCAGCC CACCACTCAG GAGCCTGAGA GCGCAAGCGA GGGGGAGGAG 
CCCCAGCGCG AGCCCTTAGA CGAGGATCCT CGGGACCCCG AGGAGTCAGA GGAGCTCAGG 
GAGGCGAACA GGCAGCCCCG CCGCTGCAAG ACCAGGAGGC CAGCCCGCCG TCGCGACCAG 
TCCCCGGAGT CCCCTCCCAG AAAGGGGCCC ATCCCCATCC GGCGTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153125.1
CDS	249..1019
Translation	MDRRSRAHQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAAQRRSFLNAHHRSAAAAAAAQVLPESSESESDHEHEEAEPELARPECLEYDQDDYETETDSETEPESDIQSETEFETEPETEPETAPTTEPETEPEDERGPRGATFNQSLTQRLHALKLQSADASPRRAQPTTQEPESASEGEEPQREPLDEDPRDPEESEELREANRQPRRCKTRRPARRRDQSPESPPRKGPIPIRRH

Target ORF information:

RefSeq Version	NM_001159653.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159653.1

ORF Insert Sequence:

ATGGATCGCA GGTCCCGGGC TCATCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCTACC GCTCTCCTCT GGCTCTCCTG CTCCATCGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGGGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCC 
GAGTCCTCTG AATCCGAATC TGATCACGAG CACGAGGAGG CTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCCAGTC CGAGACCGAA TTCGAGACCG AGCCTGAGAC CGAGCCTGAG 
ACCGCCCCTA CAACTGAGCC TGAGACCGAA CCAGAGGACG AGCGCGGCCC CCGGGGCGCC 
ACCTTCAACC AGTCACTCAC TCAGCGTCTG CACGCTCTGA AGTTGCAGAG CGCCGACGCC 
TCCCCGAGAC GTGCGCAGCC CACCACTCAG GAGCCTGAGA GCGCAAGCGA GGGGGAGGAG 
CCCCAGCGCG AGCCCTTAGA CGAGGATCCT CGGGACCCCG AGGAGTCAGA GGAGCTCAGG 
GAGGCGAACA GGCAGCCCCG CCGCTGCAAG ACCAGGAGGC CAGCCCGCCG TCGCGACCAG 
TCCCCGGAGT CCCCTCCCAG AAAGGGGCCC ATCCCCATCC GGCGTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gnas ( NM_001159656.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001153128.1 Rattus norvegicus GNAS complex locus (Gnas), transcript variant 5, mRNA.

Price & Availability↑

CloneID	ORa42795
Clone ID Related Accession (Same CDS sequence)	NM_001159653.1 , NM_001159656.1
Accession Version	NM_001159656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	SCG6 (secretogranin VI) isoform SCG6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB770483.1, AF105254.1, BC168156.1, AF107845.1 and BF394587.1. Transcript Variant: This variant (5) is maternally expressed. It lacks several 3' exons and has alternate 5' and 3' exons, as compared to variant 3. Variants 4 and 5 both encode neuroendocrine secretory protein 55 (NESP55), which localizes to large secretory vesicles of endocrine cells and neurons. The coding regions of variants 4 and 5 do not overlap the coding regions used by other transcripts; thus NESP55 has no similarity to isoforms of the G-protein alpha subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF107845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 23839232 NMD candidate :: translation inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATCGCA GGTCCCGGGC TCATCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCTACC GCTCTCCTCT GGCTCTCCTG CTCCATCGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGGGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCC 
GAGTCCTCTG AATCCGAATC TGATCACGAG CACGAGGAGG CTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCCAGTC CGAGACCGAA TTCGAGACCG AGCCTGAGAC CGAGCCTGAG 
ACCGCCCCTA CAACTGAGCC TGAGACCGAA CCAGAGGACG AGCGCGGCCC CCGGGGCGCC 
ACCTTCAACC AGTCACTCAC TCAGCGTCTG CACGCTCTGA AGTTGCAGAG CGCCGACGCC 
TCCCCGAGAC GTGCGCAGCC CACCACTCAG GAGCCTGAGA GCGCAAGCGA GGGGGAGGAG 
CCCCAGCGCG AGCCCTTAGA CGAGGATCCT CGGGACCCCG AGGAGTCAGA GGAGCTCAGG 
GAGGCGAACA GGCAGCCCCG CCGCTGCAAG ACCAGGAGGC CAGCCCGCCG TCGCGACCAG 
TCCCCGGAGT CCCCTCCCAG AAAGGGGCCC ATCCCCATCC GGCGTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153128.1
CDS	249..1019
Translation	MDRRSRAHQWRRARHNYNDLCPPIGRRAATALLWLSCSIALLRALASSNARAQQRAAQRRSFLNAHHRSAAAAAAAQVLPESSESESDHEHEEAEPELARPECLEYDQDDYETETDSETEPESDIQSETEFETEPETEPETAPTTEPETEPEDERGPRGATFNQSLTQRLHALKLQSADASPRRAQPTTQEPESASEGEEPQREPLDEDPRDPEESEELREANRQPRRCKTRRPARRRDQSPESPPRKGPIPIRRH

Target ORF information:

RefSeq Version	NM_001159656.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus GNAS complex locus (Gnas), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159656.1

ORF Insert Sequence:

ATGGATCGCA GGTCCCGGGC TCATCAGTGG CGCCGAGCTC GCCATAATTA CAACGACCTG 
TGCCCGCCCA TAGGCCGCCG GGCTGCTACC GCTCTCCTCT GGCTCTCCTG CTCCATCGCT 
CTCCTCCGCG CCCTAGCCTC TTCCAACGCC CGCGCCCAGC AGCGGGCTGC CCAGCGCCGG 
AGCTTCCTTA ACGCCCACCA CCGCTCCGCT GCCGCTGCAG CTGCCGCACA GGTACTCCCC 
GAGTCCTCTG AATCCGAATC TGATCACGAG CACGAGGAGG CTGAGCCTGA GCTGGCCCGC 
CCCGAGTGCC TAGAGTACGA TCAGGACGAC TACGAGACCG AGACCGATTC TGAGACCGAG 
CCTGAGTCCG ATATCCAGTC CGAGACCGAA TTCGAGACCG AGCCTGAGAC CGAGCCTGAG 
ACCGCCCCTA CAACTGAGCC TGAGACCGAA CCAGAGGACG AGCGCGGCCC CCGGGGCGCC 
ACCTTCAACC AGTCACTCAC TCAGCGTCTG CACGCTCTGA AGTTGCAGAG CGCCGACGCC 
TCCCCGAGAC GTGCGCAGCC CACCACTCAG GAGCCTGAGA GCGCAAGCGA GGGGGAGGAG 
CCCCAGCGCG AGCCCTTAGA CGAGGATCCT CGGGACCCCG AGGAGTCAGA GGAGCTCAGG 
GAGGCGAACA GGCAGCCCCG CCGCTGCAAG ACCAGGAGGC CAGCCCGCCG TCGCGACCAG 
TCCCCGGAGT CCCCTCCCAG AAAGGGGCCC ATCCCCATCC GGCGTCACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TGR5 contributes to hepatic cystogenesis in rodents with polycystic liver diseases through cyclic adenosine monophosphate/G?s signaling.
Hepatology (Baltimore, Md.)66(4)1197-1218(2017 10)
Masyuk TV,Masyuk AI,Lorenzo Pisarello M,Howard BN,Huang BQ,Lee PY,Fung X,Sergienko E,Ardecky RJ,Chung TDY,Pinkerton AB,LaRusso NF

The Characteristics of Hepatic Gs?-cAMP Axis in HSHF Diet-Fed Obese Insulin Resistance Rats and Genetic Diabetic Mice.
Biological & pharmaceutical bulletin40(6)774-781(2017 Jun)
Xue N,Wei C,Zhang L,Liu H,Wang X,Wang L

Antidepressants Accumulate in Lipid Rafts Independent of Monoamine Transporters to Modulate Redistribution of the G Protein, G?s.
The Journal of biological chemistry291(38)19725-19733(2016 09)
Erb SJ,Schappi JM,Rasenick MM

Activation of microtubule dynamics increases neuronal growth via the nerve growth factor (NGF)- and G?s-mediated signaling pathways.
The Journal of biological chemistry290(16)10045-56(2015 Apr)
Sarma T,Koutsouris A,Yu JZ,Krbanjevic A,Hope TJ,Rasenick MM

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Gnas cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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