Abcb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Abcb4
Entrez Gene ID	24891
Full Name	ATP binding cassette subfamily B member 4
Synonyms	Mdr2,Pgy3
General protein information	Preferred Names ATP binding cassette subfamily B member 4 Names phosphatidylcholine translocator ABCB4 ATP-binding cassette sub-family B (MDR/TAP) member 4 (P-glycoprotein 3/ multidrug resistance 2) ATP-binding cassette sub-family B member 4 ATP-binding cassette, sub-family B (MDR/TAP), member 4 (P-glycoprotein 3/ multidrug resistance 2) ATP-binding cassette, subfamily B (MDR/TAP), member 4 P-glycoprotein 2 P-glycoprotein 3 P-glycoprotein 3/ multidrug resistance 2 multidrug resistance protein 2 multidrug resistance protein 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q12
Summary	energy-dependent efflux pump; may be involved in changes in bile formation in response to diabetes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762697.2	XP_008760919.1	phosphatidylcholine translocator ABCB4 isoform X1
XM_006235999.2	XP_006236061.1	phosphatidylcholine translocator ABCB4 isoform X3
XM_017592474.1	XP_017447963.1	phosphatidylcholine translocator ABCB4 isoform X2
XM_006236000.2	XP_006236062.1	phosphatidylcholine translocator ABCB4 isoform X4
NM_012690.2	NP_036822.1	phosphatidylcholine translocator ABCB4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ABCB4	XP_003318611.1
Bos taurus (cattle)	ABCB4	XP_005205357.1
Gallus gallus (chicken)	ABCB1LA	XP_418636.4
Gallus gallus (chicken)	ABCB1LB	NP_990225.1
Homo sapiens (human)	ABCB4	NP_061337.1
Drosophila melanogaster (fruit fly)	Mdr65	NP_476831.1
Oryza sativa (rice)	Os01g0534700	NP_001172394.1
Mus musculus (house mouse)	Abcb4	NP_032856.2
Rattus norvegicus (Norway rat)	Abcb4	NP_036822.1
Drosophila melanogaster (fruit fly)	CG10226	NP_648040.1
Canis lupus familiaris (dog)	ABCB4	XP_539403.3

Gene Ontology
Bibliography

Related articles in PubMed

ABCB4 mutations in adult patients with cholestatic liver disease: impact and phenotypic expression.
Degiorgio D, Crosignani A, Colombo C, Bordo D, Zuin M, Vassallo E, Syr?n ML, Coviello DA, Battezzati PM
Journal of gastroenterology51(3)271-80(2016 Mar)

ABCB4 mutations underlie hormonal cholestasis but not pediatric idiopathic gallstones.
Jirsa M, Bronsk? J, Dvo??kov? L, ?perl J, ?majstrla V, Hor?k J, Nevoral J, H?eb??ek M
World journal of gastroenterology20(19)5867-74(2014 May)

Molecular mechanistic explanation for the spectrum of cholestatic disease caused by the S320F variant of ABCB4.
Andress EJ, Nicolaou M, Romero MR, Naik S, Dixon PH, Williamson C, Linton KJ
Hepatology (Baltimore, Md.)59(5)1921-31(2014 May)

Two ABCB4 point mutations of strategic NBD-motifs do not prevent protein targeting to the plasma membrane but promote MDR3 dysfunction.
Degiorgio D, Corsetto PA, Rizzo AM, Colombo C, Seia M, Costantino L, Montorfano G, Tomaiuolo R, Bordo D, Sansanelli S, Li M, Tavian D, Rastaldi MP, Coviello DA
European journal of human genetics : EJHG22(5)633-9(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The hyperprolactinemia-induced changes in the mrp3 levels and their correlations with prolactin receptor expression were shown to differ in the kidney and liver cells.
Title: Manifestation of multidrug resistance protein 3 (MRP3) in liver and kidney cells in cholestasis: effects of hyperprolactinemia.

Diabetes may enhance Mrp2 function and expression in liver, kidney and intestine.
Title: Induction of multidrug resistance-associated protein 2 in liver, intestine and kidney of streptozotocin-induced diabetic rats.

These findings indicate that VEGF decreases P-glycoprotein activity via activation of flk-1 and Src, and suggest Src-mediated phosphorylation of caveolin-1 may play a role in downregulation of P-glycoprotein activity.
Title: Rapid, reversible modulation of blood-brain barrier P-glycoprotein transport activity by vascular endothelial growth factor.

Transcription of rat hepatic Mrp2 mRNA is initiated at multiple sites and contains potential upstream open reading frames (uORFs) in the 5' untranslated region (UTR) starting at -213, -149 and -109 nucleotides.
Title: Translational regulation of rat multidrug resistance-associated protein 2 expression is mediated by upstream open reading frames in the 5' untranslated region.

HAX-1 binds bile salt export protein (BSEP), cortactin, MDR1, and MDR2. HAX-1 and cortactin regulate BSEP abundance in the apical membrane of cells.
Title: Identification of HAX-1 as a protein that binds bile salt export protein and regulates its abundance in the apical membrane of Madin-Darby canine kidney cells.

The following Abcb4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Abcb4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa60754	XM_017592474.1 Latest version!	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa00622	NM_012690.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$937.30 $1339.00
ORa26968	XM_008762697.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa26970	XM_006235999.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa26971	XM_006236000.2 Latest version!	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Abcb4 ( XM_017592474.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447963.1 Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60754
Clone ID Related Accession (Same CDS sequence)	XM_017592474.1
Accession Version	XM_017592474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylcholine translocator ABCB4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GCACCGCCAT GGCCATAGCT CACGGATCAG GTCTTCCCCT TATGATGATA 
GTCTTTGGAG AAATGACAGA TAAGTTTGTA GATAATGCTG GGAACTTTTC CTTGCCAGTG 
AATTTTTCAT TGTCAATGCT AAATCCAGGA AGAATTCTGG AAGAAGAAAT GACTAGATAT 
GCATACTACT ATTCGGGACT AGGTGGTGGA GTTCTTTTGG CTGCCTATAT CCAAGTCTCC 
TTCTGGACTT TGGCAGCTGG CCGACAAATA AGGAAAATCA GGCAAAAATT TTTTCACGCC 
ATCCTTCGAC AAGAAATGGG CTGGTTTGAT ATCAAGGGCA CCACCGAACT CAACACGCGG 
CTGACAGATG ACATCTCCAA AATCAGTGAA GGAATTGGTG ACAAGGTTGG AATGTTCTTT 
CAAGCAATAG CCACGTTTTT TGCAGGATTC ATAGTGGGGT TCATCAGAGG CTGGAAACTC 
ACCCTCGTGA TCATGGCCAT CAGCCCCATC TTGGGGCTCT CTACAGCCGT TTGGGCAAAG 
ATACTCTCAA CATTCAGTGA CAAAGAACTA GCTGCCTATG CAAAAGCAGG TGCCGTGGCG 
GAAGAGGCTC TGGGAGCCAT CAGGACCGTG ATAGCTTTCG GGGGCCAGAA CAAAGAGCTA 
GAAAGGTATC AGAAGCATTT AGAAAATGCC AAAAAGATTG GAATTAAAAA GGCTATCTCG 
GCCAACATTT CCATGGGCAT TGCCTTTTTG TTAATATATG CATCCTATGC ACTGGCCTTC 
TGGTACGGAT CCACTCTGGT TATATCAAAA GAATATACAA TTGGAAATGC CATGACAGTG 
TTCTTCTCAA TCCTCATTGG GGCCTTCAGT GTGGGGCAGG CTGCCCCCTG TATTGATGCC 
TTTGCCAATG CTAGAGGAGC AGCCTATGTG ATCTTTGACA TTATTGATAA TAATCCTAAA 
ATTGACAGTT TTTCAGAGAG AGGACACAAG CCAGACAGCA TCAAAGGAAA TTTGGAGTTC 
AGTGACGTTC ACTTTTCCTA CCCATCTCGG GCTAATATCA AGATCTTGAA GGGCCTCAAC 
CTGAAGGTGA AGAGCGGGCA GACGGTAGCC CTGGTTGGCA ACAGTGGCTG TGGGAAAAGC 
ACAACTGTCC AGCTGCTGCA GAGGCTCTAC GACCCCACAG AGGGTACGAT TAGCATCGAT 
GGGCAGGACA TCCGGAACTT TAACGTCAGG TGTCTAAGGG AATTCATCGG CGTGGTGAGT 
CAAGAGCCGG TACTGTTCTC TACCACGATT GCTGAAAATA TCCGCTATGG CCGTGGGAAT 
GTAACAATGG ATGAGATTAA AAAAGCTGTC AAAGAGGCCA ATGCCTATGA CTTCATCATG 
AAACTGCCAC AGAAATTTGA CACCCTGGTT GGTGACAGAG GGGCGCAGCT GAGCGGGGGA 
CAGAAACAGA GGATCGCCAT TGCTCGTGCC TTGGTCCGCA ACCCCAAGAT CCTCCTGCTG 
GACGAGGCCA CGTCAGCCTT GGACACAGAA AGCGAAGCTG AGGTGCAGGC CGCTCTGGAT 
AAGGCCAGAG AAGGCCGGAC CACCATCGTG ATAGCTCACC GACTGTCAAC TGTCCGGAAT 
GCAGATGTCA TCGCTGGGTT TGAGGATGGC GTCATCGTGG AGCAAGGAAG CCACAGTGAG 
CTGATAAAGA AGGAAGGGAT CTACTTCAGA CTTGTTAACA TGCAGACATC AGGAAGCCAG 
ATCCTGTCAG AAGAATTTGA AGTTGAGCTA AGTGATGAAA AGGCTGCTGG AGGTGTGGCC 
CCAAATGGCT GGAAAGCACG CATATTTAGG AATTCTACGA AGAAAAGTCT GAAAAGTTCA 
CGGGCGCATC AAAATAGGCT GGATGTGGAA ACCAATGAAC TTGATGCAAA CGTGCCACCA 
GTGTCTTTTC TGAAGGTCTT AAGACTGAAT AAAACAGAGT GGCCCTACTT TGTGGTGGGG 
ACACTCTGTG CCATTGCCAA CGGGGCCCTC CAGCCGGCAT TCTCCATCAT CCTGTCAGAG 
ATGATAGCTA TCTTTGGCCC TGGGGATGAC ACAGTAAAGC AACAGAAGTG TAACATGTTC 
TCGCTGGTCT TCTTGGGCCT AGGAGTCCTC TCCTTCTTTA CTTTCTTCCT TCAGGGTTTC 
ACATTCGGGA AAGCTGGCGA GATCCTCACC ACAAGGCTCC GGTCCATGGC CTTCAAAGCA 
ATGCTAAGAC AGGACATGAG CTGGTTTGAC GATCATAAAA ACAGTACTGG TGCCCTCTCT 
ACAAGACTCG CCACAGACGC TGCGCAGGTC CAAGGAGCCA CAGGAACCAG GTTGGCTTTA 
ATTGCACAGA ACACAGCCAA CCTTGGAACG GGTATTATTA TATCATTTAT TTACGGTTGG 
CAACTGACAC TTCTGCTCTT ATCAGTTGTT CCATTCATTG CTGTAGCGGG AATTGTTGAA 
ATGAAAATGT TGGCTGGCAA CGCCAAGAGA GATAAAAAGG AGATGGAAGC TGCTGGAAAG 
ATTGCAACAG AGGCAATAGA AAATATTCGG ACTGTTGTAT CCTTGACCCA AGAGAGAAAA 
TTTGAGTCAA TGTATGTTGA AAAATTACAC GGACCTTACA GGAATTCAGT GCGGAAGGCT 
CACATCTACG GCATCACTTT TAGCATCTCA CAAGCATTCA TGTACTTTTC TTATGCTGGC 
TGCTTTCGAT TTGGTTCTTA CCTCATTGTG AATGGACACA TGCGCTTCAA GGATGTCATC 
CTGGTGTTCT CAGCAATCGT GCTTGGTGCA GTGGCTCTAG GACATGCCAG CTCATTTGCT 
CCAGACTATG CAAAAGCCAA GCTGTCTGCA GCGTACTTAT TCAGTCTGTT TGAAAGACAA 
CCTCTGATTG ACAGCTACAG CAGAGAAGGA ATGTGGCCGG ATAAGTTTGA AGGAAGCGTG 
ACATTCAATG AAGTTGTGTT CAATTATCCC ACCCGGGCCA ATGTGCCAGT GCTTCAGGGG 
CTGAGCCTCG AGGTGAAGAA GGGGCAGACC CTGGCCCTGG TGGGCAGCAG TGGCTGCGGG 
AAGAGCACCG TGGTCCAGCT GCTCGAGCGC TTCTACGACC CCATGGCCGG AACAGTGCTC 
CTCGATGGTC AGGAAGCAAA GAAACTCAAT GTCCAGTGGC TCCGAGCTCA ACTTGGCATT 
GTGTCCCAGG AGCCCATCCT GTTTGACTGC AGCATCGCCG AGAACATCGC CTACGGAGAC 
AACAGCCGTG TCGTGTCTCA GGATGAGATT GTGAGGGCGG CCAAGGAGGC CAACATCCAC 
CCCTTCATTG AGACACTGCC CCAAAAGTAT GAAACAAGAG TAGGAGACAA GGGGACACAG 
CTCTCTGGAG GCCAGAAACA GAGGATTGCT ATCGCCCGAG CCCTCATCAG ACAGCCTCGG 
GTCCTACTGC TGGATGAAGC CACGTCGGCT TTGGACACTG AGAGTGAAAA GGTCGTCCAG 
GAAGCGCTGG ACAAAGCCAG GGAAGGCCGC ACCTGCATTG TGATCGCGCA CCGCCTGTCC 
ACCATCCAGA ACGCAGACTT GATCGTGGTG ATCGACAACG GCAAGGTCAA GGAGCACGGC 
ACCCACCAGC AGCTGCTGGC CCAGAAAGGC ATCTATTTCT CCATGGTCAA CATTCAAGCT 
GGCACACAGA ACTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447963.1
CDS	328..4005
Translation	MLLGTAMAIAHGSGLPLMMIVFGEMTDKFVDNAGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLLAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDISKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGTISIDGQDIRNFNVRCLREFIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIKKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELIKKEGIYFRLVNMQTSGSQILSEEFEVELSDEKAAGGVAPNGWKARIFRNSTKKSLKSSRAHQNRLDVETNELDANVPPVSFLKVLRLNKTEWPYFVVGTLCAIANGALQPAFSIILSEMIAIFGPGDDTVKQQKCNMFSLVFLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSREGMWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVRAAKEANIHPFIETLPQKYETRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIDNGKVKEHGTHQQLLAQKGIYFSMVNIQAGTQNL

Target ORF information:

RefSeq Version	XM_017592474.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592474.1

ORF Insert Sequence:

ATGCTCCTGG GCACCGCCAT GGCCATAGCT CACGGATCAG GTCTTCCCCT TATGATGATA 
GTCTTTGGAG AAATGACAGA TAAGTTTGTA GATAATGCTG GGAACTTTTC CTTGCCAGTG 
AATTTTTCAT TGTCAATGCT AAATCCAGGA AGAATTCTGG AAGAAGAAAT GACTAGATAT 
GCATACTACT ATTCGGGACT AGGTGGTGGA GTTCTTTTGG CTGCCTATAT CCAAGTCTCC 
TTCTGGACTT TGGCAGCTGG CCGACAAATA AGGAAAATCA GGCAAAAATT TTTTCACGCC 
ATCCTTCGAC AAGAAATGGG CTGGTTTGAT ATCAAGGGCA CCACCGAACT CAACACGCGG 
CTGACAGATG ACATCTCCAA AATCAGTGAA GGAATTGGTG ACAAGGTTGG AATGTTCTTT 
CAAGCAATAG CCACGTTTTT TGCAGGATTC ATAGTGGGGT TCATCAGAGG CTGGAAACTC 
ACCCTCGTGA TCATGGCCAT CAGCCCCATC TTGGGGCTCT CTACAGCCGT TTGGGCAAAG 
ATACTCTCAA CATTCAGTGA CAAAGAACTA GCTGCCTATG CAAAAGCAGG TGCCGTGGCG 
GAAGAGGCTC TGGGAGCCAT CAGGACCGTG ATAGCTTTCG GGGGCCAGAA CAAAGAGCTA 
GAAAGGTATC AGAAGCATTT AGAAAATGCC AAAAAGATTG GAATTAAAAA GGCTATCTCG 
GCCAACATTT CCATGGGCAT TGCCTTTTTG TTAATATATG CATCCTATGC ACTGGCCTTC 
TGGTACGGAT CCACTCTGGT TATATCAAAA GAATATACAA TTGGAAATGC CATGACAGTG 
TTCTTCTCAA TCCTCATTGG GGCCTTCAGT GTGGGGCAGG CTGCCCCCTG TATTGATGCC 
TTTGCCAATG CTAGAGGAGC AGCCTATGTG ATCTTTGACA TTATTGATAA TAATCCTAAA 
ATTGACAGTT TTTCAGAGAG AGGACACAAG CCAGACAGCA TCAAAGGAAA TTTGGAGTTC 
AGTGACGTTC ACTTTTCCTA CCCATCTCGG GCTAATATCA AGATCTTGAA GGGCCTCAAC 
CTGAAGGTGA AGAGCGGGCA GACGGTAGCC CTGGTTGGCA ACAGTGGCTG TGGGAAAAGC 
ACAACTGTCC AGCTGCTGCA GAGGCTCTAC GACCCCACAG AGGGTACGAT TAGCATCGAT 
GGGCAGGACA TCCGGAACTT TAACGTCAGG TGTCTAAGGG AATTCATCGG CGTGGTGAGT 
CAAGAGCCGG TACTGTTCTC TACCACGATT GCTGAAAATA TCCGCTATGG CCGTGGGAAT 
GTAACAATGG ATGAGATTAA AAAAGCTGTC AAAGAGGCCA ATGCCTATGA CTTCATCATG 
AAACTGCCAC AGAAATTTGA CACCCTGGTT GGTGACAGAG GGGCGCAGCT GAGCGGGGGA 
CAGAAACAGA GGATCGCCAT TGCTCGTGCC TTGGTCCGCA ACCCCAAGAT CCTCCTGCTG 
GACGAGGCCA CGTCAGCCTT GGACACAGAA AGCGAAGCTG AGGTGCAGGC CGCTCTGGAT 
AAGGCCAGAG AAGGCCGGAC CACCATCGTG ATAGCTCACC GACTGTCAAC TGTCCGGAAT 
GCAGATGTCA TCGCTGGGTT TGAGGATGGC GTCATCGTGG AGCAAGGAAG CCACAGTGAG 
CTGATAAAGA AGGAAGGGAT CTACTTCAGA CTTGTTAACA TGCAGACATC AGGAAGCCAG 
ATCCTGTCAG AAGAATTTGA AGTTGAGCTA AGTGATGAAA AGGCTGCTGG AGGTGTGGCC 
CCAAATGGCT GGAAAGCACG CATATTTAGG AATTCTACGA AGAAAAGTCT GAAAAGTTCA 
CGGGCGCATC AAAATAGGCT GGATGTGGAA ACCAATGAAC TTGATGCAAA CGTGCCACCA 
GTGTCTTTTC TGAAGGTCTT AAGACTGAAT AAAACAGAGT GGCCCTACTT TGTGGTGGGG 
ACACTCTGTG CCATTGCCAA CGGGGCCCTC CAGCCGGCAT TCTCCATCAT CCTGTCAGAG 
ATGATAGCTA TCTTTGGCCC TGGGGATGAC ACAGTAAAGC AACAGAAGTG TAACATGTTC 
TCGCTGGTCT TCTTGGGCCT AGGAGTCCTC TCCTTCTTTA CTTTCTTCCT TCAGGGTTTC 
ACATTCGGGA AAGCTGGCGA GATCCTCACC ACAAGGCTCC GGTCCATGGC CTTCAAAGCA 
ATGCTAAGAC AGGACATGAG CTGGTTTGAC GATCATAAAA ACAGTACTGG TGCCCTCTCT 
ACAAGACTCG CCACAGACGC TGCGCAGGTC CAAGGAGCCA CAGGAACCAG GTTGGCTTTA 
ATTGCACAGA ACACAGCCAA CCTTGGAACG GGTATTATTA TATCATTTAT TTACGGTTGG 
CAACTGACAC TTCTGCTCTT ATCAGTTGTT CCATTCATTG CTGTAGCGGG AATTGTTGAA 
ATGAAAATGT TGGCTGGCAA CGCCAAGAGA GATAAAAAGG AGATGGAAGC TGCTGGAAAG 
ATTGCAACAG AGGCAATAGA AAATATTCGG ACTGTTGTAT CCTTGACCCA AGAGAGAAAA 
TTTGAGTCAA TGTATGTTGA AAAATTACAC GGACCTTACA GGAATTCAGT GCGGAAGGCT 
CACATCTACG GCATCACTTT TAGCATCTCA CAAGCATTCA TGTACTTTTC TTATGCTGGC 
TGCTTTCGAT TTGGTTCTTA CCTCATTGTG AATGGACACA TGCGCTTCAA GGATGTCATC 
CTGGTGTTCT CAGCAATCGT GCTTGGTGCA GTGGCTCTAG GACATGCCAG CTCATTTGCT 
CCAGACTATG CAAAAGCCAA GCTGTCTGCA GCGTACTTAT TCAGTCTGTT TGAAAGACAA 
CCTCTGATTG ACAGCTACAG CAGAGAAGGA ATGTGGCCGG ATAAGTTTGA AGGAAGCGTG 
ACATTCAATG AAGTTGTGTT CAATTATCCC ACCCGGGCCA ATGTGCCAGT GCTTCAGGGG 
CTGAGCCTCG AGGTGAAGAA GGGGCAGACC CTGGCCCTGG TGGGCAGCAG TGGCTGCGGG 
AAGAGCACCG TGGTCCAGCT GCTCGAGCGC TTCTACGACC CCATGGCCGG AACAGTGCTC 
CTCGATGGTC AGGAAGCAAA GAAACTCAAT GTCCAGTGGC TCCGAGCTCA ACTTGGCATT 
GTGTCCCAGG AGCCCATCCT GTTTGACTGC AGCATCGCCG AGAACATCGC CTACGGAGAC 
AACAGCCGTG TCGTGTCTCA GGATGAGATT GTGAGGGCGG CCAAGGAGGC CAACATCCAC 
CCCTTCATTG AGACACTGCC CCAAAAGTAT GAAACAAGAG TAGGAGACAA GGGGACACAG 
CTCTCTGGAG GCCAGAAACA GAGGATTGCT ATCGCCCGAG CCCTCATCAG ACAGCCTCGG 
GTCCTACTGC TGGATGAAGC CACGTCGGCT TTGGACACTG AGAGTGAAAA GGTCGTCCAG 
GAAGCGCTGG ACAAAGCCAG GGAAGGCCGC ACCTGCATTG TGATCGCGCA CCGCCTGTCC 
ACCATCCAGA ACGCAGACTT GATCGTGGTG ATCGACAACG GCAAGGTCAA GGAGCACGGC 
ACCCACCAGC AGCTGCTGGC CCAGAAAGGC ATCTATTTCT CCATGGTCAA CATTCAAGCT 
GGCACACAGA ACTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcb4 ( NM_012690.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036822.1 Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), mRNA.

Price & Availability↑

CloneID	ORa00622
Clone ID Related Accession (Same CDS sequence)	NM_012690.2
Accession Version	NM_012690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3837bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylcholine translocator ABCB4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L15079.1. On Aug 31, 2012 this sequence version replaced NM_012690.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L15079.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGATCTTG AGGCAGCAAG AAACGGAACA GCGCGGCGCC TGGACGGCGA CTTTGAACTA 
GGCAGCATCA GCAACCAGAG CAGAGAAAAA AAGAAGAAAG TGAATTTAAT TGGCCCGTTG 
ACACTGTTCC GATACTCTGA TTGGCAGGAT AAATTGTTTA TGCTCCTGGG CACCGCCATG 
GCCATAGCTC ACGGATCAGG TCTTCCCCTT ATGATGATAG TCTTTGGAGA AATGACAGAT 
AAGTTTGTAG ATAATGCTGG GAACTTTTCC TTGCCAGTGA ATTTTTCATT GTCAATGCTA 
AATCCAGGAA GAATTCTGGA AGAAGAAATG ACTAGATATG CATACTACTA TTCGGGACTA 
GGTGGTGGAG TTCTTTTGGC TGCCTATATC CAAGTCTCCT TCTGGACTTT GGCAGCTGGC 
CGACAAATAA GGAAAATCAG GCAAAAATTT TTTCACGCCA TCCTTCGACA AGAAATGGGC 
TGGTTTGATA TCAAGGGCAC CACCGAACTC AACACGCGGC TGACAGATGA CATCTCCAAA 
ATCAGTGAAG GAATTGGTGA CAAGGTTGGA ATGTTCTTTC AAGCAATAGC CACGTTTTTT 
GCAGGATTCA TAGTGGGGTT CATCAGAGGC TGGAAACTCA CCCTCGTGAT CATGGCCATC 
ACCGCCATCT TGGGGCTCTC TACAGCCGTT TGGGCAAAGA TACTCTCAAC ATTCAGTGAC 
AAAGAACTAG CTGCCTATGC AAAAGCAGGT GCCGTGGCGG AAGAGGCTCT GGGAGCCATC 
AGGACCGTGA TAGCTTTCGG GGGCCAGAAC AAAGAGCTAG AAAGGTATCA GAAGCATTTA 
GAAAATGCCA AAAAGATTGG AATTAAAAAG GCTATCTCGG CCAACATCTC CATGGGCATT 
GCCTTTTTGT TAATATATGC ATCCTATGCA CTGGCCTTCT GGTATGGATC CACTCTGGTT 
ATATCAAAAG AATATACAAT TGGAAATGCC ATGACAGTGT TCTTCTCAAT CCTCATTGGG 
GCCTTCAGTG TGGGGCAGGC TGCCCCCTGT ATTGATGCTT TCCCCAATGC TAGAGGAGCA 
GCCTATGTGA TCTTTGACAT TATTGATAAT AATCCTAAAA TTGACAGTTT TTCAGAGAGA 
GGACACAAGC CAGACAGCAT CAAAGGAAAT TTGGAGTTCA GTGACGTTCA CTTTTCCTAC 
CCATCTCGGG CTAATATCAA GATCTTGAAG GGCCTCAACC TGAAGGTGAA GAGCGGGCAG 
ACGGTAGCCC TGGTTGGCAA CAGTGGCTGT GGGAAAAGCA CAACTGTCCA GCTGCTGCAG 
AGGCTCTACG ACCCCACAGA GGGTACGATT AGCATCGATG GGCAGGACAT CCGGAACTTT 
AACGTCAGGT GTCTAAGGGA ATTCATCGGC GTGGTGAGTC AAGAGCCGGT ACTGTTCTCT 
ACCACGATTG CTGAAAATAT CCGCTATGGC CGTGGGAATG TAACAATGGA TGAGATTAAA 
AAAGCTGTCA AAGAGGCTAA TGCCTATGAC TTCATCATGA AACTGCCACA GAAATTTGAC 
ACCCTGGTTG GTGACAGAGG GGCGCAGCTG AGCGGGGGAC AGAAACAGAG GATCGCCATT 
GCTCGTGCCT TGGTCCGCAA CCCCAAGATC CTCCTGCTGG ACGAGGCCAC GTCAGCCTTG 
GACACAGAAA GCGAAGCTGA GGTGCAGGCC GCTCTGGATA AGGCCAGAGA AGGCCGGACC 
ACCATCGTGA TAGCTCACCG ACTGTCAACT GTCCGGAATG CAGATGTCAT CGCTGGGTTT 
GAGGATGGCG TCATCGTGGA GCAAGGAAGC CACAGTGAGC TGATAAAGAA GGAAGGGATC 
TACTTCAGAC TTGTTAACAT GCAGACATCA GGAAGCCAGA TCCTGTCAGA AGAATTTGAA 
GTTGAGCTAA GTGATGAAAA GGCTGCTGGA GGTGTGGCCC CAAATGGCTG GAAAGCACGC 
ATATTTAGGA ATTCTACGAA GAAAAGTCTG AAAAGTTCAC GGGCGCATCA AAATAGGCTG 
GATGTGGAAA CCAATGAACT TGATGCAAAC GTGCCACCAG TGTCTTTTCT GAAGGTCTTA 
AGACTGAATA AAACAGAGTG GCCCTACTTT GTGGTGGGGA CACTCTGTGC CATTGCCAAC 
GGGGCCCTCC AGCCGGCATT CTCCATCATC CTGTCAGAGA TGATAGCTAT CTTTGGCCCT 
GGGGATGACA CAGTAAAGCA ACAGAAGTGT AACATGTTCT CGCTGGTCTT CTTGGGCCTA 
GGAGTCCACT CCTTCTTTAC TTTCTTCCTT CAGGGTTTCA CATTCGGGAA AGCTGGCGAG 
ATCCTCACCA CAAGGCTCCG GTCCATGGCC TTCAAAGCAA TGCTAAGACA GGACATGAGC 
TGGTTTGACG ATCATAAAAA CAGTACTGGT GCCCTCTCTA CAAGACTCGC CACAGACGCT 
GCGCAGGTCC AAGGAGCCAC AGGAACCAGG TTGGCTTTAA TTGCACAGAA CACAGCCAAC 
CTTGGAACGG GTATTATTAT ATCATTTATT TACGGTTGGC AACTGACACT TCTGCTCTTA 
TCAGTTGTTC CATTCATTGC TGTAGCGGGA ATTGTTGAAA TGAAAATGTT GGCTGGCAAC 
GCCAAGAGAG ATAAAAAGGA GATGGAAGCT GCTGGAAAGA TTGCAACAGA GGCAATAGAA 
AATATTCGGA CTGTTGTATC CTTGACCCAA GAGAGAAAAT TTGAGTCAAT GTATGTTGAA 
AAATTACACG GACCTTACAG GAATTCAGTG CGGAAGGCTC ACATCTACGG CATCACTTTT 
AGCATCTCAC AAGCATTCAT GTACTTTTCT TATGCTGGCT GCTTTCGATT TGGTTCTTAC 
CTCATTGTGA ATGGACACAT GCGCTTCAAG GATGTCATCC TGGTGTTCTC AGCAATCGTG 
CTTGGTGCAG TGGCTCTAGG ACATGCCAGC TCATTTGCTC CAGACTATGC AAAAGCCAAG 
CTGTCTGCAG CATACTTATT CAGTCTGTTT GAAAGACAAC CTCTGATTGA CAGCTACAGC 
AGAGAAGGAA TGTGGCCGGA TAAGTTTGAA GGAAGCGTGA CATTCAATGA AGTTGTGTTC 
AATTATCCCA CCCGGGCCAA TGTGCCAGTG CTTCAGGGGC TGAGCCTCGA GGTGAAGAAG 
GGGCAGACCC TGGCCCTGGT GGGCAGTAGT GGCTGCGGGA AGAGCACCGT GGTCCAGCTG 
CTCGAGCGCT TCTACGACCC CATGGCCGGA ACAGTGCTCC TCGATGGTCA GGAAGCAAAG 
AAACTCAATG TCCAGTGGCT CCGAGCTCAA CTTGGCATTG TGTCCCAGGA GCCCATCCTG 
TTTGACTGCA GCATCGCCAA GAACATCGCC TACGGAGACA ACAGCCGTGT CGTGTCTCAG 
GATGAGATTG TGAGGGCGGC CAAGGAGGCC AACATCCACC CCTTCATTGA GACACTGCCC 
CAAAAGTATG AAACAAGAGT AGGAGACAAG GGGACACAGC TCTCTGGAGG CCAGAAACAG 
AGGATTGCTA TCGCCCGAGC CCTCATCAGA CAGCCTCGGG TCCTACTGCT GGATGAAGCC 
ACGTCGGCTT TGGACACTGA GAGTGAAAAG GTCGTCCAGG AAGCGCTGGA CAAAGCCAGG 
GAAGGCCGCA CCTGCATTGT GATCGCGCAC CGCCTGTCCA CCATCCAGAA CGCAGACTTG 
ATCGTGGTGA TCGACAACGG CAAGGTCAAG GAGCACGGCA CCCACCAGCA GCTGCTGGCC 
CAGAAAGGCA TCTATTTCTC CATGGTCAAC ATTCAAGCTG GCACACAGAA CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036822.1
CDS	31..3867
Translation	MDLEAARNGTARRLDGDFELGSISNQSREKKKKVNLIGPLTLFRYSDWQDKLFMLLGTAMAIAHGSGLPLMMIVFGEMTDKFVDNAGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLLAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDISKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAITAILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFPNARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGTISIDGQDIRNFNVRCLREFIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIKKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELIKKEGIYFRLVNMQTSGSQILSEEFEVELSDEKAAGGVAPNGWKARIFRNSTKKSLKSSRAHQNRLDVETNELDANVPPVSFLKVLRLNKTEWPYFVVGTLCAIANGALQPAFSIILSEMIAIFGPGDDTVKQQKCNMFSLVFLGLGVHSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSREGMWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAKNIAYGDNSRVVSQDEIVRAAKEANIHPFIETLPQKYETRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIDNGKVKEHGTHQQLLAQKGIYFSMVNIQAGTQNL

Target ORF information:

RefSeq Version	NM_012690.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012690.2

ORF Insert Sequence:

ATGGATCTTG AGGCAGCAAG AAACGGAACA GCGCGGCGCC TGGACGGCGA CTTTGAACTA 
GGCAGCATCA GCAACCAGAG CAGAGAAAAA AAGAAGAAAG TGAATTTAAT TGGCCCGTTG 
ACACTGTTCC GATACTCTGA TTGGCAGGAT AAATTGTTTA TGCTCCTGGG CACCGCCATG 
GCCATAGCTC ACGGATCAGG TCTTCCCCTT ATGATGATAG TCTTTGGAGA AATGACAGAT 
AAGTTTGTAG ATAATGCTGG GAACTTTTCC TTGCCAGTGA ATTTTTCATT GTCAATGCTA 
AATCCAGGAA GAATTCTGGA AGAAGAAATG ACTAGATATG CATACTACTA TTCGGGACTA 
GGTGGTGGAG TTCTTTTGGC TGCCTATATC CAAGTCTCCT TCTGGACTTT GGCAGCTGGC 
CGACAAATAA GGAAAATCAG GCAAAAATTT TTTCACGCCA TCCTTCGACA AGAAATGGGC 
TGGTTTGATA TCAAGGGCAC CACCGAACTC AACACGCGGC TGACAGATGA CATCTCCAAA 
ATCAGTGAAG GAATTGGTGA CAAGGTTGGA ATGTTCTTTC AAGCAATAGC CACGTTTTTT 
GCAGGATTCA TAGTGGGGTT CATCAGAGGC TGGAAACTCA CCCTCGTGAT CATGGCCATC 
ACCGCCATCT TGGGGCTCTC TACAGCCGTT TGGGCAAAGA TACTCTCAAC ATTCAGTGAC 
AAAGAACTAG CTGCCTATGC AAAAGCAGGT GCCGTGGCGG AAGAGGCTCT GGGAGCCATC 
AGGACCGTGA TAGCTTTCGG GGGCCAGAAC AAAGAGCTAG AAAGGTATCA GAAGCATTTA 
GAAAATGCCA AAAAGATTGG AATTAAAAAG GCTATCTCGG CCAACATCTC CATGGGCATT 
GCCTTTTTGT TAATATATGC ATCCTATGCA CTGGCCTTCT GGTATGGATC CACTCTGGTT 
ATATCAAAAG AATATACAAT TGGAAATGCC ATGACAGTGT TCTTCTCAAT CCTCATTGGG 
GCCTTCAGTG TGGGGCAGGC TGCCCCCTGT ATTGATGCTT TCCCCAATGC TAGAGGAGCA 
GCCTATGTGA TCTTTGACAT TATTGATAAT AATCCTAAAA TTGACAGTTT TTCAGAGAGA 
GGACACAAGC CAGACAGCAT CAAAGGAAAT TTGGAGTTCA GTGACGTTCA CTTTTCCTAC 
CCATCTCGGG CTAATATCAA GATCTTGAAG GGCCTCAACC TGAAGGTGAA GAGCGGGCAG 
ACGGTAGCCC TGGTTGGCAA CAGTGGCTGT GGGAAAAGCA CAACTGTCCA GCTGCTGCAG 
AGGCTCTACG ACCCCACAGA GGGTACGATT AGCATCGATG GGCAGGACAT CCGGAACTTT 
AACGTCAGGT GTCTAAGGGA ATTCATCGGC GTGGTGAGTC AAGAGCCGGT ACTGTTCTCT 
ACCACGATTG CTGAAAATAT CCGCTATGGC CGTGGGAATG TAACAATGGA TGAGATTAAA 
AAAGCTGTCA AAGAGGCTAA TGCCTATGAC TTCATCATGA AACTGCCACA GAAATTTGAC 
ACCCTGGTTG GTGACAGAGG GGCGCAGCTG AGCGGGGGAC AGAAACAGAG GATCGCCATT 
GCTCGTGCCT TGGTCCGCAA CCCCAAGATC CTCCTGCTGG ACGAGGCCAC GTCAGCCTTG 
GACACAGAAA GCGAAGCTGA GGTGCAGGCC GCTCTGGATA AGGCCAGAGA AGGCCGGACC 
ACCATCGTGA TAGCTCACCG ACTGTCAACT GTCCGGAATG CAGATGTCAT CGCTGGGTTT 
GAGGATGGCG TCATCGTGGA GCAAGGAAGC CACAGTGAGC TGATAAAGAA GGAAGGGATC 
TACTTCAGAC TTGTTAACAT GCAGACATCA GGAAGCCAGA TCCTGTCAGA AGAATTTGAA 
GTTGAGCTAA GTGATGAAAA GGCTGCTGGA GGTGTGGCCC CAAATGGCTG GAAAGCACGC 
ATATTTAGGA ATTCTACGAA GAAAAGTCTG AAAAGTTCAC GGGCGCATCA AAATAGGCTG 
GATGTGGAAA CCAATGAACT TGATGCAAAC GTGCCACCAG TGTCTTTTCT GAAGGTCTTA 
AGACTGAATA AAACAGAGTG GCCCTACTTT GTGGTGGGGA CACTCTGTGC CATTGCCAAC 
GGGGCCCTCC AGCCGGCATT CTCCATCATC CTGTCAGAGA TGATAGCTAT CTTTGGCCCT 
GGGGATGACA CAGTAAAGCA ACAGAAGTGT AACATGTTCT CGCTGGTCTT CTTGGGCCTA 
GGAGTCCACT CCTTCTTTAC TTTCTTCCTT CAGGGTTTCA CATTCGGGAA AGCTGGCGAG 
ATCCTCACCA CAAGGCTCCG GTCCATGGCC TTCAAAGCAA TGCTAAGACA GGACATGAGC 
TGGTTTGACG ATCATAAAAA CAGTACTGGT GCCCTCTCTA CAAGACTCGC CACAGACGCT 
GCGCAGGTCC AAGGAGCCAC AGGAACCAGG TTGGCTTTAA TTGCACAGAA CACAGCCAAC 
CTTGGAACGG GTATTATTAT ATCATTTATT TACGGTTGGC AACTGACACT TCTGCTCTTA 
TCAGTTGTTC CATTCATTGC TGTAGCGGGA ATTGTTGAAA TGAAAATGTT GGCTGGCAAC 
GCCAAGAGAG ATAAAAAGGA GATGGAAGCT GCTGGAAAGA TTGCAACAGA GGCAATAGAA 
AATATTCGGA CTGTTGTATC CTTGACCCAA GAGAGAAAAT TTGAGTCAAT GTATGTTGAA 
AAATTACACG GACCTTACAG GAATTCAGTG CGGAAGGCTC ACATCTACGG CATCACTTTT 
AGCATCTCAC AAGCATTCAT GTACTTTTCT TATGCTGGCT GCTTTCGATT TGGTTCTTAC 
CTCATTGTGA ATGGACACAT GCGCTTCAAG GATGTCATCC TGGTGTTCTC AGCAATCGTG 
CTTGGTGCAG TGGCTCTAGG ACATGCCAGC TCATTTGCTC CAGACTATGC AAAAGCCAAG 
CTGTCTGCAG CATACTTATT CAGTCTGTTT GAAAGACAAC CTCTGATTGA CAGCTACAGC 
AGAGAAGGAA TGTGGCCGGA TAAGTTTGAA GGAAGCGTGA CATTCAATGA AGTTGTGTTC 
AATTATCCCA CCCGGGCCAA TGTGCCAGTG CTTCAGGGGC TGAGCCTCGA GGTGAAGAAG 
GGGCAGACCC TGGCCCTGGT GGGCAGTAGT GGCTGCGGGA AGAGCACCGT GGTCCAGCTG 
CTCGAGCGCT TCTACGACCC CATGGCCGGA ACAGTGCTCC TCGATGGTCA GGAAGCAAAG 
AAACTCAATG TCCAGTGGCT CCGAGCTCAA CTTGGCATTG TGTCCCAGGA GCCCATCCTG 
TTTGACTGCA GCATCGCCAA GAACATCGCC TACGGAGACA ACAGCCGTGT CGTGTCTCAG 
GATGAGATTG TGAGGGCGGC CAAGGAGGCC AACATCCACC CCTTCATTGA GACACTGCCC 
CAAAAGTATG AAACAAGAGT AGGAGACAAG GGGACACAGC TCTCTGGAGG CCAGAAACAG 
AGGATTGCTA TCGCCCGAGC CCTCATCAGA CAGCCTCGGG TCCTACTGCT GGATGAAGCC 
ACGTCGGCTT TGGACACTGA GAGTGAAAAG GTCGTCCAGG AAGCGCTGGA CAAAGCCAGG 
GAAGGCCGCA CCTGCATTGT GATCGCGCAC CGCCTGTCCA CCATCCAGAA CGCAGACTTG 
ATCGTGGTGA TCGACAACGG CAAGGTCAAG GAGCACGGCA CCCACCAGCA GCTGCTGGCC 
CAGAAAGGCA TCTATTTCTC CATGGTCAAC ATTCAAGCTG GCACACAGAA CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcb4 ( XM_008762697.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760919.1 Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X1, mRNA.

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CloneID	ORa26968
Clone ID Related Accession (Same CDS sequence)	XM_008762697.2
Accession Version	XM_008762697.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylcholine translocator ABCB4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTTG AGGCAGCAAG AAACGGAACA GCGCGGCGCC TGGACGGCGA CTTTGAACTA 
GGCAGCATCA GCAACCAGAG CAGAGAAAAA AAGAAGAAAG TGAATTTAAT TGGCCCGTTG 
ACACTGTTCC GATACTCTGA TTGGCAGGAT AAATTGTTTA TGCTCCTGGG CACCGCCATG 
GCCATAGCTC ACGGATCAGG TCTTCCCCTT ATGATGATAG TCTTTGGAGA AATGACAGAT 
AAGTTTGTAG ATAATGCTGG GAACTTTTCC TTGCCAGTGA ATTTTTCATT GTCAATGCTA 
AATCCAGGAA GAATTCTGGA AGAAGAAATG ACTAGATATG CATACTACTA TTCGGGACTA 
GGTGGTGGAG TTCTTTTGGC TGCCTATATC CAAGTCTCCT TCTGGACTTT GGCAGCTGGC 
CGACAAATAA GGAAAATCAG GCAAAAATTT TTTCACGCCA TCCTTCGACA AGAAATGGGC 
TGGTTTGATA TCAAGGGCAC CACCGAACTC AACACGCGGC TGACAGATGA CATCTCCAAA 
ATCAGTGAAG GAATTGGTGA CAAGGTTGGA ATGTTCTTTC AAGCAATAGC CACGTTTTTT 
GCAGGATTCA TAGTGGGGTT CATCAGAGGC TGGAAACTCA CCCTCGTGAT CATGGCCATC 
AGCCCCATCT TGGGGCTCTC TACAGCCGTT TGGGCAAAGA TACTCTCAAC ATTCAGTGAC 
AAAGAACTAG CTGCCTATGC AAAAGCAGGT GCCGTGGCGG AAGAGGCTCT GGGAGCCATC 
AGGACCGTGA TAGCTTTCGG GGGCCAGAAC AAAGAGCTAG AAAGGTATCA GAAGCATTTA 
GAAAATGCCA AAAAGATTGG AATTAAAAAG GCTATCTCGG CCAACATTTC CATGGGCATT 
GCCTTTTTGT TAATATATGC ATCCTATGCA CTGGCCTTCT GGTACGGATC CACTCTGGTT 
ATATCAAAAG AATATACAAT TGGAAATGCC ATGACAGTGT TCTTCTCAAT CCTCATTGGG 
GCCTTCAGTG TGGGGCAGGC TGCCCCCTGT ATTGATGCCT TTGCCAATGC TAGAGGAGCA 
GCCTATGTGA TCTTTGACAT TATTGATAAT AATCCTAAAA TTGACAGTTT TTCAGAGAGA 
GGACACAAGC CAGACAGCAT CAAAGGAAAT TTGGAGTTCA GTGACGTTCA CTTTTCCTAC 
CCATCTCGGG CTAATATCAA GATCTTGAAG GGCCTCAACC TGAAGGTGAA GAGCGGGCAG 
ACGGTAGCCC TGGTTGGCAA CAGTGGCTGT GGGAAAAGCA CAACTGTCCA GCTGCTGCAG 
AGGCTCTACG ACCCCACAGA GGGTACGATT AGCATCGATG GGCAGGACAT CCGGAACTTT 
AACGTCAGGT GTCTAAGGGA ATTCATCGGC GTGGTGAGTC AAGAGCCGGT ACTGTTCTCT 
ACCACGATTG CTGAAAATAT CCGCTATGGC CGTGGGAATG TAACAATGGA TGAGATTAAA 
AAAGCTGTCA AAGAGGCCAA TGCCTATGAC TTCATCATGA AACTGCCACA GAAATTTGAC 
ACCCTGGTTG GTGACAGAGG GGCGCAGCTG AGCGGGGGAC AGAAACAGAG GATCGCCATT 
GCTCGTGCCT TGGTCCGCAA CCCCAAGATC CTCCTGCTGG ACGAGGCCAC GTCAGCCTTG 
GACACAGAAA GCGAAGCTGA GGTGCAGGCC GCTCTGGATA AGGCCAGAGA AGGCCGGACC 
ACCATCGTGA TAGCTCACCG ACTGTCAACT GTCCGGAATG CAGATGTCAT CGCTGGGTTT 
GAGGATGGCG TCATCGTGGA GCAAGGAAGC CACAGTGAGC TGATAAAGAA GGAAGGGATC 
TACTTCAGAC TTGTTAACAT GCAGACATCA GGAAGCCAGA TCCTGTCAGA AGAATTTGAA 
GTTGAGCTAA GTGATGAAAA GGCTGCTGGA GGTGTGGCCC CAAATGGCTG GAAAGCACGC 
ATATTTAGGA ATTCTACGAA GAAAAGTCTG AAAAGTTCAC GGGCGCATCA AAATAGGCTG 
GATGTGGAAA CCAATGAACT TGATGCAAAC GTGCCACCAG TGTCTTTTCT GAAGGTCTTA 
AGACTGAATA AAACAGAGTG GCCCTACTTT GTGGTGGGGA CACTCTGTGC CATTGCCAAC 
GGGGCCCTCC AGCCGGCATT CTCCATCATC CTGTCAGAGA TGATAGCTGG TTTCACATTC 
GGGAAAGCTG GCGAGATCCT CACCACAAGG CTCCGGTCCA TGGCCTTCAA AGCAATGCTA 
AGACAGGACA TGAGCTGGTT TGACGATCAT AAAAACAGTA CTGGTGCCCT CTCTACAAGA 
CTCGCCACAG ACGCTGCGCA GGTCCAAGGA GCCACAGGAA CCAGGTTGGC TTTAATTGCA 
CAGAACACAG CCAACCTTGG AACGGGTATT ATTATATCAT TTATTTACGG TTGGCAACTG 
ACACTTCTGC TCTTATCAGT TGTTCCATTC ATTGCTGTAG CGGGAATTGT TGAAATGAAA 
ATGTTGGCTG GCAACGCCAA GAGAGATAAA AAGGAGATGG AAGCTGCTGG AAAGATTGCA 
ACAGAGGCAA TAGAAAATAT TCGGACTGTT GTATCCTTGA CCCAAGAGAG AAAATTTGAG 
TCAATGTATG TTGAAAAATT ACACGGACCT TACAGGAATT CAGTGCGGAA GGCTCACATC 
TACGGCATCA CTTTTAGCAT CTCACAAGCA TTCATGTACT TTTCTTATGC TGGCTGCTTT 
CGATTTGGTT CTTACCTCAT TGTGAATGGA CACATGCGCT TCAAGGATGT CATCCTGGTG 
TTCTCAGCAA TCGTGCTTGG TGCAGTGGCT CTAGGACATG CCAGCTCATT TGCTCCAGAC 
TATGCAAAAG CCAAGCTGTC TGCAGCGTAC TTATTCAGTC TGTTTGAAAG ACAACCTCTG 
ATTGACAGCT ACAGCAGAGA AGGAATGTGG CCGGATAAGT TTGAAGGAAG CGTGACATTC 
AATGAAGTTG TGTTCAATTA TCCCACCCGG GCCAATGTGC CAGTGCTTCA GGGGCTGAGC 
CTCGAGGTGA AGAAGGGGCA GACCCTGGCC CTGGTGGGCA GCAGTGGCTG CGGGAAGAGC 
ACCGTGGTCC AGCTGCTCGA GCGCTTCTAC GACCCCATGG CCGGAACAGT GCTCCTCGAT 
GGTCAGGAAG CAAAGAAACT CAATGTCCAG TGGCTCCGAG CTCAACTTGG CATTGTGTCC 
CAGGAGCCCA TCCTGTTTGA CTGCAGCATC GCCGAGAACA TCGCCTACGG AGACAACAGC 
CGTGTCGTGT CTCAGGATGA GATTGTGAGG GCGGCCAAGG AGGCCAACAT CCACCCCTTC 
ATTGAGACAC TGCCCCAAAA GTATGAAACA AGAGTAGGAG ACAAGGGGAC ACAGCTCTCT 
GGAGGCCAGA AACAGAGGAT TGCTATCGCC CGAGCCCTCA TCAGACAGCC TCGGGTCCTA 
CTGCTGGATG AAGCCACGTC GGCTTTGGAC ACTGAGAGTG AAAAGGTCGT CCAGGAAGCG 
CTGGACAAAG CCAGGGAAGG CCGCACCTGC ATTGTGATCG CGCACCGCCT GTCCACCATC 
CAGAACGCAG ACTTGATCGT GGTGATCGAC AACGGCAAGG TCAAGGAGCA CGGCACCCAC 
CAGCAGCTGC TGGCCCAGAA AGGCATCTAT TTCTCCATGG TCAACATTCA AGCTGGCACA 
CAGAACTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760919.1
CDS	218..3949
Translation	MDLEAARNGTARRLDGDFELGSISNQSREKKKKVNLIGPLTLFRYSDWQDKLFMLLGTAMAIAHGSGLPLMMIVFGEMTDKFVDNAGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLLAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDISKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGTISIDGQDIRNFNVRCLREFIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIKKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELIKKEGIYFRLVNMQTSGSQILSEEFEVELSDEKAAGGVAPNGWKARIFRNSTKKSLKSSRAHQNRLDVETNELDANVPPVSFLKVLRLNKTEWPYFVVGTLCAIANGALQPAFSIILSEMIAGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSREGMWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVRAAKEANIHPFIETLPQKYETRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIDNGKVKEHGTHQQLLAQKGIYFSMVNIQAGTQNL

Target ORF information:

RefSeq Version	XM_008762697.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762697.2

ORF Insert Sequence:

ATGGATCTTG AGGCAGCAAG AAACGGAACA GCGCGGCGCC TGGACGGCGA CTTTGAACTA 
GGCAGCATCA GCAACCAGAG CAGAGAAAAA AAGAAGAAAG TGAATTTAAT TGGCCCGTTG 
ACACTGTTCC GATACTCTGA TTGGCAGGAT AAATTGTTTA TGCTCCTGGG CACCGCCATG 
GCCATAGCTC ACGGATCAGG TCTTCCCCTT ATGATGATAG TCTTTGGAGA AATGACAGAT 
AAGTTTGTAG ATAATGCTGG GAACTTTTCC TTGCCAGTGA ATTTTTCATT GTCAATGCTA 
AATCCAGGAA GAATTCTGGA AGAAGAAATG ACTAGATATG CATACTACTA TTCGGGACTA 
GGTGGTGGAG TTCTTTTGGC TGCCTATATC CAAGTCTCCT TCTGGACTTT GGCAGCTGGC 
CGACAAATAA GGAAAATCAG GCAAAAATTT TTTCACGCCA TCCTTCGACA AGAAATGGGC 
TGGTTTGATA TCAAGGGCAC CACCGAACTC AACACGCGGC TGACAGATGA CATCTCCAAA 
ATCAGTGAAG GAATTGGTGA CAAGGTTGGA ATGTTCTTTC AAGCAATAGC CACGTTTTTT 
GCAGGATTCA TAGTGGGGTT CATCAGAGGC TGGAAACTCA CCCTCGTGAT CATGGCCATC 
AGCCCCATCT TGGGGCTCTC TACAGCCGTT TGGGCAAAGA TACTCTCAAC ATTCAGTGAC 
AAAGAACTAG CTGCCTATGC AAAAGCAGGT GCCGTGGCGG AAGAGGCTCT GGGAGCCATC 
AGGACCGTGA TAGCTTTCGG GGGCCAGAAC AAAGAGCTAG AAAGGTATCA GAAGCATTTA 
GAAAATGCCA AAAAGATTGG AATTAAAAAG GCTATCTCGG CCAACATTTC CATGGGCATT 
GCCTTTTTGT TAATATATGC ATCCTATGCA CTGGCCTTCT GGTACGGATC CACTCTGGTT 
ATATCAAAAG AATATACAAT TGGAAATGCC ATGACAGTGT TCTTCTCAAT CCTCATTGGG 
GCCTTCAGTG TGGGGCAGGC TGCCCCCTGT ATTGATGCCT TTGCCAATGC TAGAGGAGCA 
GCCTATGTGA TCTTTGACAT TATTGATAAT AATCCTAAAA TTGACAGTTT TTCAGAGAGA 
GGACACAAGC CAGACAGCAT CAAAGGAAAT TTGGAGTTCA GTGACGTTCA CTTTTCCTAC 
CCATCTCGGG CTAATATCAA GATCTTGAAG GGCCTCAACC TGAAGGTGAA GAGCGGGCAG 
ACGGTAGCCC TGGTTGGCAA CAGTGGCTGT GGGAAAAGCA CAACTGTCCA GCTGCTGCAG 
AGGCTCTACG ACCCCACAGA GGGTACGATT AGCATCGATG GGCAGGACAT CCGGAACTTT 
AACGTCAGGT GTCTAAGGGA ATTCATCGGC GTGGTGAGTC AAGAGCCGGT ACTGTTCTCT 
ACCACGATTG CTGAAAATAT CCGCTATGGC CGTGGGAATG TAACAATGGA TGAGATTAAA 
AAAGCTGTCA AAGAGGCCAA TGCCTATGAC TTCATCATGA AACTGCCACA GAAATTTGAC 
ACCCTGGTTG GTGACAGAGG GGCGCAGCTG AGCGGGGGAC AGAAACAGAG GATCGCCATT 
GCTCGTGCCT TGGTCCGCAA CCCCAAGATC CTCCTGCTGG ACGAGGCCAC GTCAGCCTTG 
GACACAGAAA GCGAAGCTGA GGTGCAGGCC GCTCTGGATA AGGCCAGAGA AGGCCGGACC 
ACCATCGTGA TAGCTCACCG ACTGTCAACT GTCCGGAATG CAGATGTCAT CGCTGGGTTT 
GAGGATGGCG TCATCGTGGA GCAAGGAAGC CACAGTGAGC TGATAAAGAA GGAAGGGATC 
TACTTCAGAC TTGTTAACAT GCAGACATCA GGAAGCCAGA TCCTGTCAGA AGAATTTGAA 
GTTGAGCTAA GTGATGAAAA GGCTGCTGGA GGTGTGGCCC CAAATGGCTG GAAAGCACGC 
ATATTTAGGA ATTCTACGAA GAAAAGTCTG AAAAGTTCAC GGGCGCATCA AAATAGGCTG 
GATGTGGAAA CCAATGAACT TGATGCAAAC GTGCCACCAG TGTCTTTTCT GAAGGTCTTA 
AGACTGAATA AAACAGAGTG GCCCTACTTT GTGGTGGGGA CACTCTGTGC CATTGCCAAC 
GGGGCCCTCC AGCCGGCATT CTCCATCATC CTGTCAGAGA TGATAGCTGG TTTCACATTC 
GGGAAAGCTG GCGAGATCCT CACCACAAGG CTCCGGTCCA TGGCCTTCAA AGCAATGCTA 
AGACAGGACA TGAGCTGGTT TGACGATCAT AAAAACAGTA CTGGTGCCCT CTCTACAAGA 
CTCGCCACAG ACGCTGCGCA GGTCCAAGGA GCCACAGGAA CCAGGTTGGC TTTAATTGCA 
CAGAACACAG CCAACCTTGG AACGGGTATT ATTATATCAT TTATTTACGG TTGGCAACTG 
ACACTTCTGC TCTTATCAGT TGTTCCATTC ATTGCTGTAG CGGGAATTGT TGAAATGAAA 
ATGTTGGCTG GCAACGCCAA GAGAGATAAA AAGGAGATGG AAGCTGCTGG AAAGATTGCA 
ACAGAGGCAA TAGAAAATAT TCGGACTGTT GTATCCTTGA CCCAAGAGAG AAAATTTGAG 
TCAATGTATG TTGAAAAATT ACACGGACCT TACAGGAATT CAGTGCGGAA GGCTCACATC 
TACGGCATCA CTTTTAGCAT CTCACAAGCA TTCATGTACT TTTCTTATGC TGGCTGCTTT 
CGATTTGGTT CTTACCTCAT TGTGAATGGA CACATGCGCT TCAAGGATGT CATCCTGGTG 
TTCTCAGCAA TCGTGCTTGG TGCAGTGGCT CTAGGACATG CCAGCTCATT TGCTCCAGAC 
TATGCAAAAG CCAAGCTGTC TGCAGCGTAC TTATTCAGTC TGTTTGAAAG ACAACCTCTG 
ATTGACAGCT ACAGCAGAGA AGGAATGTGG CCGGATAAGT TTGAAGGAAG CGTGACATTC 
AATGAAGTTG TGTTCAATTA TCCCACCCGG GCCAATGTGC CAGTGCTTCA GGGGCTGAGC 
CTCGAGGTGA AGAAGGGGCA GACCCTGGCC CTGGTGGGCA GCAGTGGCTG CGGGAAGAGC 
ACCGTGGTCC AGCTGCTCGA GCGCTTCTAC GACCCCATGG CCGGAACAGT GCTCCTCGAT 
GGTCAGGAAG CAAAGAAACT CAATGTCCAG TGGCTCCGAG CTCAACTTGG CATTGTGTCC 
CAGGAGCCCA TCCTGTTTGA CTGCAGCATC GCCGAGAACA TCGCCTACGG AGACAACAGC 
CGTGTCGTGT CTCAGGATGA GATTGTGAGG GCGGCCAAGG AGGCCAACAT CCACCCCTTC 
ATTGAGACAC TGCCCCAAAA GTATGAAACA AGAGTAGGAG ACAAGGGGAC ACAGCTCTCT 
GGAGGCCAGA AACAGAGGAT TGCTATCGCC CGAGCCCTCA TCAGACAGCC TCGGGTCCTA 
CTGCTGGATG AAGCCACGTC GGCTTTGGAC ACTGAGAGTG AAAAGGTCGT CCAGGAAGCG 
CTGGACAAAG CCAGGGAAGG CCGCACCTGC ATTGTGATCG CGCACCGCCT GTCCACCATC 
CAGAACGCAG ACTTGATCGT GGTGATCGAC AACGGCAAGG TCAAGGAGCA CGGCACCCAC 
CAGCAGCTGC TGGCCCAGAA AGGCATCTAT TTCTCCATGG TCAACATTCA AGCTGGCACA 
CAGAACTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcb4 ( XM_006235999.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236061.1 Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X3, mRNA.

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CloneID	ORa26970
Clone ID Related Accession (Same CDS sequence)	XM_006235999.2
Accession Version	XM_006235999.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylcholine translocator ABCB4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006235999.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAGTG CCTGCCTTCT CCTTGCTCAA CAATGCTTCA GGGATATTTT ATTCAGTAAT 
ATATTTCACA AGTGCTTGAA TTTTTCATTG TCAATGCTAA ATCCAGGAAG AATTCTGGAA 
GAAGAAATGA CTAGATATGC ATACTACTAT TCGGGACTAG GTGGTGGAGT TCTTTTGGCT 
GCCTATATCC AAGTCTCCTT CTGGACTTTG GCAGCTGGCC GACAAATAAG GAAAATCAGG 
CAAAAATTTT TTCACGCCAT CCTTCGACAA GAAATGGGCT GGTTTGATAT CAAGGGCACC 
ACCGAACTCA ACACGCGGCT GACAGATGAC ATCTCCAAAA TCAGTGAAGG AATTGGTGAC 
AAGGTTGGAA TGTTCTTTCA AGCAATAGCC ACGTTTTTTG CAGGATTCAT AGTGGGGTTC 
ATCAGAGGCT GGAAACTCAC CCTCGTGATC ATGGCCATCA GCCCCATCTT GGGGCTCTCT 
ACAGCCGTTT GGGCAAAGAT ACTCTCAACA TTCAGTGACA AAGAACTAGC TGCCTATGCA 
AAAGCAGGTG CCGTGGCGGA AGAGGCTCTG GGAGCCATCA GGACCGTGAT AGCTTTCGGG 
GGCCAGAACA AAGAGCTAGA AAGGTATCAG AAGCATTTAG AAAATGCCAA AAAGATTGGA 
ATTAAAAAGG CTATCTCGGC CAACATTTCC ATGGGCATTG CCTTTTTGTT AATATATGCA 
TCCTATGCAC TGGCCTTCTG GTACGGATCC ACTCTGGTTA TATCAAAAGA ATATACAATT 
GGAAATGCCA TGACAGTGTT CTTCTCAATC CTCATTGGGG CCTTCAGTGT GGGGCAGGCT 
GCCCCCTGTA TTGATGCCTT TGCCAATGCT AGAGGAGCAG CCTATGTGAT CTTTGACATT 
ATTGATAATA ATCCTAAAAT TGACAGTTTT TCAGAGAGAG GACACAAGCC AGACAGCATC 
AAAGGAAATT TGGAGTTCAG TGACGTTCAC TTTTCCTACC CATCTCGGGC TAATATCAAG 
ATCTTGAAGG GCCTCAACCT GAAGGTGAAG AGCGGGCAGA CGGTAGCCCT GGTTGGCAAC 
AGTGGCTGTG GGAAAAGCAC AACTGTCCAG CTGCTGCAGA GGCTCTACGA CCCCACAGAG 
GGTACGATTA GCATCGATGG GCAGGACATC CGGAACTTTA ACGTCAGGTG TCTAAGGGAA 
TTCATCGGCG TGGTGAGTCA AGAGCCGGTA CTGTTCTCTA CCACGATTGC TGAAAATATC 
CGCTATGGCC GTGGGAATGT AACAATGGAT GAGATTAAAA AAGCTGTCAA AGAGGCCAAT 
GCCTATGACT TCATCATGAA ACTGCCACAG AAATTTGACA CCCTGGTTGG TGACAGAGGG 
GCGCAGCTGA GCGGGGGACA GAAACAGAGG ATCGCCATTG CTCGTGCCTT GGTCCGCAAC 
CCCAAGATCC TCCTGCTGGA CGAGGCCACG TCAGCCTTGG ACACAGAAAG CGAAGCTGAG 
GTGCAGGCCG CTCTGGATAA GGCCAGAGAA GGCCGGACCA CCATCGTGAT AGCTCACCGA 
CTGTCAACTG TCCGGAATGC AGATGTCATC GCTGGGTTTG AGGATGGCGT CATCGTGGAG 
CAAGGAAGCC ACAGTGAGCT GATAAAGAAG GAAGGGATCT ACTTCAGACT TGTTAACATG 
CAGACATCAG GAAGCCAGAT CCTGTCAGAA GAATTTGAAG TTGAGCTAAG TGATGAAAAG 
GCTGCTGGAG GTGTGGCCCC AAATGGCTGG AAAGCACGCA TATTTAGGAA TTCTACGAAG 
AAAAGTCTGA AAAGTTCACG GGCGCATCAA AATAGGCTGG ATGTGGAAAC CAATGAACTT 
GATGCAAACG TGCCACCAGT GTCTTTTCTG AAGGTCTTAA GACTGAATAA AACAGAGTGG 
CCCTACTTTG TGGTGGGGAC ACTCTGTGCC ATTGCCAACG GGGCCCTCCA GCCGGCATTC 
TCCATCATCC TGTCAGAGAT GATAGCTATC TTTGGCCCTG GGGATGACAC AGTAAAGCAA 
CAGAAGTGTA ACATGTTCTC GCTGGTCTTC TTGGGCCTAG GAGTCCTCTC CTTCTTTACT 
TTCTTCCTTC AGGGTTTCAC ATTCGGGAAA GCTGGCGAGA TCCTCACCAC AAGGCTCCGG 
TCCATGGCCT TCAAAGCAAT GCTAAGACAG GACATGAGCT GGTTTGACGA TCATAAAAAC 
AGTACTGGTG CCCTCTCTAC AAGACTCGCC ACAGACGCTG CGCAGGTCCA AGGAGCCACA 
GGAACCAGGT TGGCTTTAAT TGCACAGAAC ACAGCCAACC TTGGAACGGG TATTATTATA 
TCATTTATTT ACGGTTGGCA ACTGACACTT CTGCTCTTAT CAGTTGTTCC ATTCATTGCT 
GTAGCGGGAA TTGTTGAAAT GAAAATGTTG GCTGGCAACG CCAAGAGAGA TAAAAAGGAG 
ATGGAAGCTG CTGGAAAGAT TGCAACAGAG GCAATAGAAA ATATTCGGAC TGTTGTATCC 
TTGACCCAAG AGAGAAAATT TGAGTCAATG TATGTTGAAA AATTACACGG ACCTTACAGG 
AATTCAGTGC GGAAGGCTCA CATCTACGGC ATCACTTTTA GCATCTCACA AGCATTCATG 
TACTTTTCTT ATGCTGGCTG CTTTCGATTT GGTTCTTACC TCATTGTGAA TGGACACATG 
CGCTTCAAGG ATGTCATCCT GGTGTTCTCA GCAATCGTGC TTGGTGCAGT GGCTCTAGGA 
CATGCCAGCT CATTTGCTCC AGACTATGCA AAAGCCAAGC TGTCTGCAGC GTACTTATTC 
AGTCTGTTTG AAAGACAACC TCTGATTGAC AGCTACAGCA GAGAAGGAAT GTGGCCGGAT 
AAGTTTGAAG GAAGCGTGAC ATTCAATGAA GTTGTGTTCA ATTATCCCAC CCGGGCCAAT 
GTGCCAGTGC TTCAGGGGCT GAGCCTCGAG GTGAAGAAGG GGCAGACCCT GGCCCTGGTG 
GGCAGCAGTG GCTGCGGGAA GAGCACCGTG GTCCAGCTGC TCGAGCGCTT CTACGACCCC 
ATGGCCGGAA CAGTGCTCCT CGATGGTCAG GAAGCAAAGA AACTCAATGT CCAGTGGCTC 
CGAGCTCAAC TTGGCATTGT GTCCCAGGAG CCCATCCTGT TTGACTGCAG CATCGCCGAG 
AACATCGCCT ACGGAGACAA CAGCCGTGTC GTGTCTCAGG ATGAGATTGT GAGGGCGGCC 
AAGGAGGCCA ACATCCACCC CTTCATTGAG ACACTGCCCC AAAAGTATGA AACAAGAGTA 
GGAGACAAGG GGACACAGCT CTCTGGAGGC CAGAAACAGA GGATTGCTAT CGCCCGAGCC 
CTCATCAGAC AGCCTCGGGT CCTACTGCTG GATGAAGCCA CGTCGGCTTT GGACACTGAG 
AGTGAAAAGG TCGTCCAGGA AGCGCTGGAC AAAGCCAGGG AAGGCCGCAC CTGCATTGTG 
ATCGCGCACC GCCTGTCCAC CATCCAGAAC GCAGACTTGA TCGTGGTGAT CGACAACGGC 
AAGGTCAAGG AGCACGGCAC CCACCAGCAG CTGCTGGCCC AGAAAGGCAT CTATTTCTCC 
ATGGTCAACA TTCAAGCTGG CACACAGAAC TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236061.1
CDS	292..3927
Translation	MGSACLLLAQQCFRDILFSNIFHKCLNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLLAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDISKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGTISIDGQDIRNFNVRCLREFIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIKKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELIKKEGIYFRLVNMQTSGSQILSEEFEVELSDEKAAGGVAPNGWKARIFRNSTKKSLKSSRAHQNRLDVETNELDANVPPVSFLKVLRLNKTEWPYFVVGTLCAIANGALQPAFSIILSEMIAIFGPGDDTVKQQKCNMFSLVFLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSREGMWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVRAAKEANIHPFIETLPQKYETRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIDNGKVKEHGTHQQLLAQKGIYFSMVNIQAGTQNL

Target ORF information:

RefSeq Version	XM_006235999.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235999.2

ORF Insert Sequence:

ATGGGAAGTG CCTGCCTTCT CCTTGCTCAA CAATGCTTCA GGGATATTTT ATTCAGTAAT 
ATATTTCACA AGTGCTTGAA TTTTTCATTG TCAATGCTAA ATCCAGGAAG AATTCTGGAA 
GAAGAAATGA CTAGATATGC ATACTACTAT TCGGGACTAG GTGGTGGAGT TCTTTTGGCT 
GCCTATATCC AAGTCTCCTT CTGGACTTTG GCAGCTGGCC GACAAATAAG GAAAATCAGG 
CAAAAATTTT TTCACGCCAT CCTTCGACAA GAAATGGGCT GGTTTGATAT CAAGGGCACC 
ACCGAACTCA ACACGCGGCT GACAGATGAC ATCTCCAAAA TCAGTGAAGG AATTGGTGAC 
AAGGTTGGAA TGTTCTTTCA AGCAATAGCC ACGTTTTTTG CAGGATTCAT AGTGGGGTTC 
ATCAGAGGCT GGAAACTCAC CCTCGTGATC ATGGCCATCA GCCCCATCTT GGGGCTCTCT 
ACAGCCGTTT GGGCAAAGAT ACTCTCAACA TTCAGTGACA AAGAACTAGC TGCCTATGCA 
AAAGCAGGTG CCGTGGCGGA AGAGGCTCTG GGAGCCATCA GGACCGTGAT AGCTTTCGGG 
GGCCAGAACA AAGAGCTAGA AAGGTATCAG AAGCATTTAG AAAATGCCAA AAAGATTGGA 
ATTAAAAAGG CTATCTCGGC CAACATTTCC ATGGGCATTG CCTTTTTGTT AATATATGCA 
TCCTATGCAC TGGCCTTCTG GTACGGATCC ACTCTGGTTA TATCAAAAGA ATATACAATT 
GGAAATGCCA TGACAGTGTT CTTCTCAATC CTCATTGGGG CCTTCAGTGT GGGGCAGGCT 
GCCCCCTGTA TTGATGCCTT TGCCAATGCT AGAGGAGCAG CCTATGTGAT CTTTGACATT 
ATTGATAATA ATCCTAAAAT TGACAGTTTT TCAGAGAGAG GACACAAGCC AGACAGCATC 
AAAGGAAATT TGGAGTTCAG TGACGTTCAC TTTTCCTACC CATCTCGGGC TAATATCAAG 
ATCTTGAAGG GCCTCAACCT GAAGGTGAAG AGCGGGCAGA CGGTAGCCCT GGTTGGCAAC 
AGTGGCTGTG GGAAAAGCAC AACTGTCCAG CTGCTGCAGA GGCTCTACGA CCCCACAGAG 
GGTACGATTA GCATCGATGG GCAGGACATC CGGAACTTTA ACGTCAGGTG TCTAAGGGAA 
TTCATCGGCG TGGTGAGTCA AGAGCCGGTA CTGTTCTCTA CCACGATTGC TGAAAATATC 
CGCTATGGCC GTGGGAATGT AACAATGGAT GAGATTAAAA AAGCTGTCAA AGAGGCCAAT 
GCCTATGACT TCATCATGAA ACTGCCACAG AAATTTGACA CCCTGGTTGG TGACAGAGGG 
GCGCAGCTGA GCGGGGGACA GAAACAGAGG ATCGCCATTG CTCGTGCCTT GGTCCGCAAC 
CCCAAGATCC TCCTGCTGGA CGAGGCCACG TCAGCCTTGG ACACAGAAAG CGAAGCTGAG 
GTGCAGGCCG CTCTGGATAA GGCCAGAGAA GGCCGGACCA CCATCGTGAT AGCTCACCGA 
CTGTCAACTG TCCGGAATGC AGATGTCATC GCTGGGTTTG AGGATGGCGT CATCGTGGAG 
CAAGGAAGCC ACAGTGAGCT GATAAAGAAG GAAGGGATCT ACTTCAGACT TGTTAACATG 
CAGACATCAG GAAGCCAGAT CCTGTCAGAA GAATTTGAAG TTGAGCTAAG TGATGAAAAG 
GCTGCTGGAG GTGTGGCCCC AAATGGCTGG AAAGCACGCA TATTTAGGAA TTCTACGAAG 
AAAAGTCTGA AAAGTTCACG GGCGCATCAA AATAGGCTGG ATGTGGAAAC CAATGAACTT 
GATGCAAACG TGCCACCAGT GTCTTTTCTG AAGGTCTTAA GACTGAATAA AACAGAGTGG 
CCCTACTTTG TGGTGGGGAC ACTCTGTGCC ATTGCCAACG GGGCCCTCCA GCCGGCATTC 
TCCATCATCC TGTCAGAGAT GATAGCTATC TTTGGCCCTG GGGATGACAC AGTAAAGCAA 
CAGAAGTGTA ACATGTTCTC GCTGGTCTTC TTGGGCCTAG GAGTCCTCTC CTTCTTTACT 
TTCTTCCTTC AGGGTTTCAC ATTCGGGAAA GCTGGCGAGA TCCTCACCAC AAGGCTCCGG 
TCCATGGCCT TCAAAGCAAT GCTAAGACAG GACATGAGCT GGTTTGACGA TCATAAAAAC 
AGTACTGGTG CCCTCTCTAC AAGACTCGCC ACAGACGCTG CGCAGGTCCA AGGAGCCACA 
GGAACCAGGT TGGCTTTAAT TGCACAGAAC ACAGCCAACC TTGGAACGGG TATTATTATA 
TCATTTATTT ACGGTTGGCA ACTGACACTT CTGCTCTTAT CAGTTGTTCC ATTCATTGCT 
GTAGCGGGAA TTGTTGAAAT GAAAATGTTG GCTGGCAACG CCAAGAGAGA TAAAAAGGAG 
ATGGAAGCTG CTGGAAAGAT TGCAACAGAG GCAATAGAAA ATATTCGGAC TGTTGTATCC 
TTGACCCAAG AGAGAAAATT TGAGTCAATG TATGTTGAAA AATTACACGG ACCTTACAGG 
AATTCAGTGC GGAAGGCTCA CATCTACGGC ATCACTTTTA GCATCTCACA AGCATTCATG 
TACTTTTCTT ATGCTGGCTG CTTTCGATTT GGTTCTTACC TCATTGTGAA TGGACACATG 
CGCTTCAAGG ATGTCATCCT GGTGTTCTCA GCAATCGTGC TTGGTGCAGT GGCTCTAGGA 
CATGCCAGCT CATTTGCTCC AGACTATGCA AAAGCCAAGC TGTCTGCAGC GTACTTATTC 
AGTCTGTTTG AAAGACAACC TCTGATTGAC AGCTACAGCA GAGAAGGAAT GTGGCCGGAT 
AAGTTTGAAG GAAGCGTGAC ATTCAATGAA GTTGTGTTCA ATTATCCCAC CCGGGCCAAT 
GTGCCAGTGC TTCAGGGGCT GAGCCTCGAG GTGAAGAAGG GGCAGACCCT GGCCCTGGTG 
GGCAGCAGTG GCTGCGGGAA GAGCACCGTG GTCCAGCTGC TCGAGCGCTT CTACGACCCC 
ATGGCCGGAA CAGTGCTCCT CGATGGTCAG GAAGCAAAGA AACTCAATGT CCAGTGGCTC 
CGAGCTCAAC TTGGCATTGT GTCCCAGGAG CCCATCCTGT TTGACTGCAG CATCGCCGAG 
AACATCGCCT ACGGAGACAA CAGCCGTGTC GTGTCTCAGG ATGAGATTGT GAGGGCGGCC 
AAGGAGGCCA ACATCCACCC CTTCATTGAG ACACTGCCCC AAAAGTATGA AACAAGAGTA 
GGAGACAAGG GGACACAGCT CTCTGGAGGC CAGAAACAGA GGATTGCTAT CGCCCGAGCC 
CTCATCAGAC AGCCTCGGGT CCTACTGCTG GATGAAGCCA CGTCGGCTTT GGACACTGAG 
AGTGAAAAGG TCGTCCAGGA AGCGCTGGAC AAAGCCAGGG AAGGCCGCAC CTGCATTGTG 
ATCGCGCACC GCCTGTCCAC CATCCAGAAC GCAGACTTGA TCGTGGTGAT CGACAACGGC 
AAGGTCAAGG AGCACGGCAC CCACCAGCAG CTGCTGGCCC AGAAAGGCAT CTATTTCTCC 
ATGGTCAACA TTCAAGCTGG CACACAGAAC TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Abcb4 ( XM_006236000.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236062.1 Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa26971
Clone ID Related Accession (Same CDS sequence)	XM_006236000.2
Accession Version	XM_006236000.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidylcholine translocator ABCB4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGGT TTGATATCAA GGGCACCACC GAACTCAACA CGCGGCTGAC AGATGACATC 
TCCAAAATCA GTGAAGGAAT TGGTGACAAG GTTGGAATGT TCTTTCAAGC AATAGCCACG 
TTTTTTGCAG GATTCATAGT GGGGTTCATC AGAGGCTGGA AACTCACCCT CGTGATCATG 
GCCATCAGCC CCATCTTGGG GCTCTCTACA GCCGTTTGGG CAAAGATACT CTCAACATTC 
AGTGACAAAG AACTAGCTGC CTATGCAAAA GCAGGTGCCG TGGCGGAAGA GGCTCTGGGA 
GCCATCAGGA CCGTGATAGC TTTCGGGGGC CAGAACAAAG AGCTAGAAAG GTATCAGAAG 
CATTTAGAAA ATGCCAAAAA GATTGGAATT AAAAAGGCTA TCTCGGCCAA CATTTCCATG 
GGCATTGCCT TTTTGTTAAT ATATGCATCC TATGCACTGG CCTTCTGGTA CGGATCCACT 
CTGGTTATAT CAAAAGAATA TACAATTGGA AATGCCATGA CAGTGTTCTT CTCAATCCTC 
ATTGGGGCCT TCAGTGTGGG GCAGGCTGCC CCCTGTATTG ATGCCTTTGC CAATGCTAGA 
GGAGCAGCCT ATGTGATCTT TGACATTATT GATAATAATC CTAAAATTGA CAGTTTTTCA 
GAGAGAGGAC ACAAGCCAGA CAGCATCAAA GGAAATTTGG AGTTCAGTGA CGTTCACTTT 
TCCTACCCAT CTCGGGCTAA TATCAAGATC TTGAAGGGCC TCAACCTGAA GGTGAAGAGC 
GGGCAGACGG TAGCCCTGGT TGGCAACAGT GGCTGTGGGA AAAGCACAAC TGTCCAGCTG 
CTGCAGAGGC TCTACGACCC CACAGAGGGT ACGATTAGCA TCGATGGGCA GGACATCCGG 
AACTTTAACG TCAGGTGTCT AAGGGAATTC ATCGGCGTGG TGAGTCAAGA GCCGGTACTG 
TTCTCTACCA CGATTGCTGA AAATATCCGC TATGGCCGTG GGAATGTAAC AATGGATGAG 
ATTAAAAAAG CTGTCAAAGA GGCCAATGCC TATGACTTCA TCATGAAACT GCCACAGAAA 
TTTGACACCC TGGTTGGTGA CAGAGGGGCG CAGCTGAGCG GGGGACAGAA ACAGAGGATC 
GCCATTGCTC GTGCCTTGGT CCGCAACCCC AAGATCCTCC TGCTGGACGA GGCCACGTCA 
GCCTTGGACA CAGAAAGCGA AGCTGAGGTG CAGGCCGCTC TGGATAAGGC CAGAGAAGGC 
CGGACCACCA TCGTGATAGC TCACCGACTG TCAACTGTCC GGAATGCAGA TGTCATCGCT 
GGGTTTGAGG ATGGCGTCAT CGTGGAGCAA GGAAGCCACA GTGAGCTGAT AAAGAAGGAA 
GGGATCTACT TCAGACTTGT TAACATGCAG ACATCAGGAA GCCAGATCCT GTCAGAAGAA 
TTTGAAGTTG AGCTAAGTGA TGAAAAGGCT GCTGGAGGTG TGGCCCCAAA TGGCTGGAAA 
GCACGCATAT TTAGGAATTC TACGAAGAAA AGTCTGAAAA GTTCACGGGC GCATCAAAAT 
AGGCTGGATG TGGAAACCAA TGAACTTGAT GCAAACGTGC CACCAGTGTC TTTTCTGAAG 
GTCTTAAGAC TGAATAAAAC AGAGTGGCCC TACTTTGTGG TGGGGACACT CTGTGCCATT 
GCCAACGGGG CCCTCCAGCC GGCATTCTCC ATCATCCTGT CAGAGATGAT AGCTATCTTT 
GGCCCTGGGG ATGACACAGT AAAGCAACAG AAGTGTAACA TGTTCTCGCT GGTCTTCTTG 
GGCCTAGGAG TCCTCTCCTT CTTTACTTTC TTCCTTCAGG GTTTCACATT CGGGAAAGCT 
GGCGAGATCC TCACCACAAG GCTCCGGTCC ATGGCCTTCA AAGCAATGCT AAGACAGGAC 
ATGAGCTGGT TTGACGATCA TAAAAACAGT ACTGGTGCCC TCTCTACAAG ACTCGCCACA 
GACGCTGCGC AGGTCCAAGG AGCCACAGGA ACCAGGTTGG CTTTAATTGC ACAGAACACA 
GCCAACCTTG GAACGGGTAT TATTATATCA TTTATTTACG GTTGGCAACT GACACTTCTG 
CTCTTATCAG TTGTTCCATT CATTGCTGTA GCGGGAATTG TTGAAATGAA AATGTTGGCT 
GGCAACGCCA AGAGAGATAA AAAGGAGATG GAAGCTGCTG GAAAGATTGC AACAGAGGCA 
ATAGAAAATA TTCGGACTGT TGTATCCTTG ACCCAAGAGA GAAAATTTGA GTCAATGTAT 
GTTGAAAAAT TACACGGACC TTACAGGAAT TCAGTGCGGA AGGCTCACAT CTACGGCATC 
ACTTTTAGCA TCTCACAAGC ATTCATGTAC TTTTCTTATG CTGGCTGCTT TCGATTTGGT 
TCTTACCTCA TTGTGAATGG ACACATGCGC TTCAAGGATG TCATCCTGGT GTTCTCAGCA 
ATCGTGCTTG GTGCAGTGGC TCTAGGACAT GCCAGCTCAT TTGCTCCAGA CTATGCAAAA 
GCCAAGCTGT CTGCAGCGTA CTTATTCAGT CTGTTTGAAA GACAACCTCT GATTGACAGC 
TACAGCAGAG AAGGAATGTG GCCGGATAAG TTTGAAGGAA GCGTGACATT CAATGAAGTT 
GTGTTCAATT ATCCCACCCG GGCCAATGTG CCAGTGCTTC AGGGGCTGAG CCTCGAGGTG 
AAGAAGGGGC AGACCCTGGC CCTGGTGGGC AGCAGTGGCT GCGGGAAGAG CACCGTGGTC 
CAGCTGCTCG AGCGCTTCTA CGACCCCATG GCCGGAACAG TGCTCCTCGA TGGTCAGGAA 
GCAAAGAAAC TCAATGTCCA GTGGCTCCGA GCTCAACTTG GCATTGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT CGCCGAGAAC ATCGCCTACG GAGACAACAG CCGTGTCGTG 
TCTCAGGATG AGATTGTGAG GGCGGCCAAG GAGGCCAACA TCCACCCCTT CATTGAGACA 
CTGCCCCAAA AGTATGAAAC AAGAGTAGGA GACAAGGGGA CACAGCTCTC TGGAGGCCAG 
AAACAGAGGA TTGCTATCGC CCGAGCCCTC ATCAGACAGC CTCGGGTCCT ACTGCTGGAT 
GAAGCCACGT CGGCTTTGGA CACTGAGAGT GAAAAGGTCG TCCAGGAAGC GCTGGACAAA 
GCCAGGGAAG GCCGCACCTG CATTGTGATC GCGCACCGCC TGTCCACCAT CCAGAACGCA 
GACTTGATCG TGGTGATCGA CAACGGCAAG GTCAAGGAGC ACGGCACCCA CCAGCAGCTG 
CTGGCCCAGA AAGGCATCTA TTTCTCCATG GTCAACATTC AAGCTGGCAC ACAGAACTTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236062.1
CDS	225..3587
Translation	MGWFDIKGTTELNTRLTDDISKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGTISIDGQDIRNFNVRCLREFIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIKKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIAGFEDGVIVEQGSHSELIKKEGIYFRLVNMQTSGSQILSEEFEVELSDEKAAGGVAPNGWKARIFRNSTKKSLKSSRAHQNRLDVETNELDANVPPVSFLKVLRLNKTEWPYFVVGTLCAIANGALQPAFSIILSEMIAIFGPGDDTVKQQKCNMFSLVFLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQAFMYFSYAGCFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSREGMWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVRAAKEANIHPFIETLPQKYETRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVIDNGKVKEHGTHQQLLAQKGIYFSMVNIQAGTQNL

Target ORF information:

RefSeq Version	XM_006236000.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP binding cassette subfamily B member 4 (Abcb4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236000.2

ORF Insert Sequence:

ATGGGCTGGT TTGATATCAA GGGCACCACC GAACTCAACA CGCGGCTGAC AGATGACATC 
TCCAAAATCA GTGAAGGAAT TGGTGACAAG GTTGGAATGT TCTTTCAAGC AATAGCCACG 
TTTTTTGCAG GATTCATAGT GGGGTTCATC AGAGGCTGGA AACTCACCCT CGTGATCATG 
GCCATCAGCC CCATCTTGGG GCTCTCTACA GCCGTTTGGG CAAAGATACT CTCAACATTC 
AGTGACAAAG AACTAGCTGC CTATGCAAAA GCAGGTGCCG TGGCGGAAGA GGCTCTGGGA 
GCCATCAGGA CCGTGATAGC TTTCGGGGGC CAGAACAAAG AGCTAGAAAG GTATCAGAAG 
CATTTAGAAA ATGCCAAAAA GATTGGAATT AAAAAGGCTA TCTCGGCCAA CATTTCCATG 
GGCATTGCCT TTTTGTTAAT ATATGCATCC TATGCACTGG CCTTCTGGTA CGGATCCACT 
CTGGTTATAT CAAAAGAATA TACAATTGGA AATGCCATGA CAGTGTTCTT CTCAATCCTC 
ATTGGGGCCT TCAGTGTGGG GCAGGCTGCC CCCTGTATTG ATGCCTTTGC CAATGCTAGA 
GGAGCAGCCT ATGTGATCTT TGACATTATT GATAATAATC CTAAAATTGA CAGTTTTTCA 
GAGAGAGGAC ACAAGCCAGA CAGCATCAAA GGAAATTTGG AGTTCAGTGA CGTTCACTTT 
TCCTACCCAT CTCGGGCTAA TATCAAGATC TTGAAGGGCC TCAACCTGAA GGTGAAGAGC 
GGGCAGACGG TAGCCCTGGT TGGCAACAGT GGCTGTGGGA AAAGCACAAC TGTCCAGCTG 
CTGCAGAGGC TCTACGACCC CACAGAGGGT ACGATTAGCA TCGATGGGCA GGACATCCGG 
AACTTTAACG TCAGGTGTCT AAGGGAATTC ATCGGCGTGG TGAGTCAAGA GCCGGTACTG 
TTCTCTACCA CGATTGCTGA AAATATCCGC TATGGCCGTG GGAATGTAAC AATGGATGAG 
ATTAAAAAAG CTGTCAAAGA GGCCAATGCC TATGACTTCA TCATGAAACT GCCACAGAAA 
TTTGACACCC TGGTTGGTGA CAGAGGGGCG CAGCTGAGCG GGGGACAGAA ACAGAGGATC 
GCCATTGCTC GTGCCTTGGT CCGCAACCCC AAGATCCTCC TGCTGGACGA GGCCACGTCA 
GCCTTGGACA CAGAAAGCGA AGCTGAGGTG CAGGCCGCTC TGGATAAGGC CAGAGAAGGC 
CGGACCACCA TCGTGATAGC TCACCGACTG TCAACTGTCC GGAATGCAGA TGTCATCGCT 
GGGTTTGAGG ATGGCGTCAT CGTGGAGCAA GGAAGCCACA GTGAGCTGAT AAAGAAGGAA 
GGGATCTACT TCAGACTTGT TAACATGCAG ACATCAGGAA GCCAGATCCT GTCAGAAGAA 
TTTGAAGTTG AGCTAAGTGA TGAAAAGGCT GCTGGAGGTG TGGCCCCAAA TGGCTGGAAA 
GCACGCATAT TTAGGAATTC TACGAAGAAA AGTCTGAAAA GTTCACGGGC GCATCAAAAT 
AGGCTGGATG TGGAAACCAA TGAACTTGAT GCAAACGTGC CACCAGTGTC TTTTCTGAAG 
GTCTTAAGAC TGAATAAAAC AGAGTGGCCC TACTTTGTGG TGGGGACACT CTGTGCCATT 
GCCAACGGGG CCCTCCAGCC GGCATTCTCC ATCATCCTGT CAGAGATGAT AGCTATCTTT 
GGCCCTGGGG ATGACACAGT AAAGCAACAG AAGTGTAACA TGTTCTCGCT GGTCTTCTTG 
GGCCTAGGAG TCCTCTCCTT CTTTACTTTC TTCCTTCAGG GTTTCACATT CGGGAAAGCT 
GGCGAGATCC TCACCACAAG GCTCCGGTCC ATGGCCTTCA AAGCAATGCT AAGACAGGAC 
ATGAGCTGGT TTGACGATCA TAAAAACAGT ACTGGTGCCC TCTCTACAAG ACTCGCCACA 
GACGCTGCGC AGGTCCAAGG AGCCACAGGA ACCAGGTTGG CTTTAATTGC ACAGAACACA 
GCCAACCTTG GAACGGGTAT TATTATATCA TTTATTTACG GTTGGCAACT GACACTTCTG 
CTCTTATCAG TTGTTCCATT CATTGCTGTA GCGGGAATTG TTGAAATGAA AATGTTGGCT 
GGCAACGCCA AGAGAGATAA AAAGGAGATG GAAGCTGCTG GAAAGATTGC AACAGAGGCA 
ATAGAAAATA TTCGGACTGT TGTATCCTTG ACCCAAGAGA GAAAATTTGA GTCAATGTAT 
GTTGAAAAAT TACACGGACC TTACAGGAAT TCAGTGCGGA AGGCTCACAT CTACGGCATC 
ACTTTTAGCA TCTCACAAGC ATTCATGTAC TTTTCTTATG CTGGCTGCTT TCGATTTGGT 
TCTTACCTCA TTGTGAATGG ACACATGCGC TTCAAGGATG TCATCCTGGT GTTCTCAGCA 
ATCGTGCTTG GTGCAGTGGC TCTAGGACAT GCCAGCTCAT TTGCTCCAGA CTATGCAAAA 
GCCAAGCTGT CTGCAGCGTA CTTATTCAGT CTGTTTGAAA GACAACCTCT GATTGACAGC 
TACAGCAGAG AAGGAATGTG GCCGGATAAG TTTGAAGGAA GCGTGACATT CAATGAAGTT 
GTGTTCAATT ATCCCACCCG GGCCAATGTG CCAGTGCTTC AGGGGCTGAG CCTCGAGGTG 
AAGAAGGGGC AGACCCTGGC CCTGGTGGGC AGCAGTGGCT GCGGGAAGAG CACCGTGGTC 
CAGCTGCTCG AGCGCTTCTA CGACCCCATG GCCGGAACAG TGCTCCTCGA TGGTCAGGAA 
GCAAAGAAAC TCAATGTCCA GTGGCTCCGA GCTCAACTTG GCATTGTGTC CCAGGAGCCC 
ATCCTGTTTG ACTGCAGCAT CGCCGAGAAC ATCGCCTACG GAGACAACAG CCGTGTCGTG 
TCTCAGGATG AGATTGTGAG GGCGGCCAAG GAGGCCAACA TCCACCCCTT CATTGAGACA 
CTGCCCCAAA AGTATGAAAC AAGAGTAGGA GACAAGGGGA CACAGCTCTC TGGAGGCCAG 
AAACAGAGGA TTGCTATCGC CCGAGCCCTC ATCAGACAGC CTCGGGTCCT ACTGCTGGAT 
GAAGCCACGT CGGCTTTGGA CACTGAGAGT GAAAAGGTCG TCCAGGAAGC GCTGGACAAA 
GCCAGGGAAG GCCGCACCTG CATTGTGATC GCGCACCGCC TGTCCACCAT CCAGAACGCA 
GACTTGATCG TGGTGATCGA CAACGGCAAG GTCAAGGAGC ACGGCACCCA CCAGCAGCTG 
CTGGCCCAGA AAGGCATCTA TTTCTCCATG GTCAACATTC AAGCTGGCAC ACAGAACTTA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ABCB4 mutations in adult patients with cholestatic liver disease: impact and phenotypic expression.
Journal of gastroenterology51(3)271-80(2016 Mar)
Degiorgio D,Crosignani A,Colombo C,Bordo D,Zuin M,Vassallo E,Syr?n ML,Coviello DA,Battezzati PM

ABCB4 mutations underlie hormonal cholestasis but not pediatric idiopathic gallstones.
World journal of gastroenterology20(19)5867-74(2014 May)
Jirsa M,Bronsk? J,Dvo??kov? L,?perl J,?majstrla V,Hor?k J,Nevoral J,H?eb??ek M

Molecular mechanistic explanation for the spectrum of cholestatic disease caused by the S320F variant of ABCB4.
Hepatology (Baltimore, Md.)59(5)1921-31(2014 May)
Andress EJ,Nicolaou M,Romero MR,Naik S,Dixon PH,Williamson C,Linton KJ

Two ABCB4 point mutations of strategic NBD-motifs do not prevent protein targeting to the plasma membrane but promote MDR3 dysfunction.
European journal of human genetics : EJHG22(5)633-9(2014 May)
Degiorgio D,Corsetto PA,Rizzo AM,Colombo C,Seia M,Costantino L,Montorfano G,Tomaiuolo R,Bordo D,Sansanelli S,Li M,Tavian D,Rastaldi MP,Coviello DA

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Abcb4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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