Wt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wt1
Entrez Gene ID	24883
Full Name	Wilms tumor 1
General protein information	Preferred Names Wilms tumor 1 Names Wilms tumor protein homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q33
Summary	This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It plays an essential role in the normal development of the urogenital system, and the human gene is mutated in a small subset of patients with Wilm's tumors. Authors of PMID:7926762 provide evidence that Wt1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234620.1	XP_006234682.1	Wilms tumor protein homolog isoform X1
XM_006234622.3	XP_006234684.1	Wilms tumor protein homolog isoform X3
XM_017591476.1	XP_017446965.1	Wilms tumor protein homolog isoform X4
XM_006234621.3	XP_006234683.1	Wilms tumor protein homolog isoform X2
XM_017591477.1	XP_017446966.1	Wilms tumor protein homolog isoform X5
NM_031534.2	NP_113722.2	Wilms tumor protein homolog

Pathways from BioSystems

Homologies

Bos taurus (cattle)	WT1	XP_003587050.2
Homo sapiens (human)	WT1	NP_077742.2
Mus musculus (house mouse)	Wt1	NP_659032.3
Gallus gallus (chicken)	WT1	NP_990547.1
Danio rerio (zebrafish)	wt1a	NP_571121.1
	WT1	XP_001084704.2
Rattus norvegicus (Norway rat)	Wt1	NP_113722.2
Xenopus tropicalis (tropical clawed frog)	wt1	NP_001135625.1
Pan troglodytes (chimpanzee)	WT1	XP_001138640.3
Canis lupus familiaris (dog)	WT1	XP_005631206.1

Gene Ontology
Bibliography

Related articles in PubMed

WT1 ameliorates podocyte injury via repression of EZH2/?-catenin pathway in diabetic nephropathy.
Wan J, Hou X, Zhou Z, Geng J, Tian J, Bai X, Nie J
Free radical biology & medicine108280-299(2017 Jul)

Changes in renal WT-1 expression preceding hypertension development.
Mazzei L, Garc?a M, Calvo JP, Casarotto M, Forn?s M, Abud MA, Cuello-Carri?n D, Ferder L, Manucha W
BMC nephrology1734(2016 Mar)

Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence.
Hashimoto H, Olanrewaju YO, Zheng Y, Wilson GG, Zhang X, Cheng X
Genes & development28(20)2304-13(2014 Oct)

Intrauterine growth restriction leads to a dysregulation of Wilms' tumour supressor gene 1 (WT1) and to early podocyte alterations.
Menendez-Castro C, Hilgers KF, Amann K, Daniel C, Cordasic N, Wachtveitl R, Fahlbusch F, Plank C, D?tsch J, Rascher W, Hartner A
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association28(6)1407-17(2013 Jun)

Retinoids augment the expression of podocyte proteins by glomerular parietal epithelial cells in experimental glomerular disease.
Zhang J, Pippin JW, Vaughan MR, Krofft RD, Taniguchi Y, Romagnani P, Nelson PJ, Liu ZH, Shankland SJ
Nephron. Experimental nephrology121(1-2)e23-37(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Renal WT1 expression is decreased in spontaneously hypertensive rats before the development of hypertension.
Title: Changes in renal WT-1 expression preceding hypertension development.

High WT1 protein is associated with reduced ability to maintain podocyte integrity, rendering intrauterine growth restriction rats more susceptible for renal disease.
Title: Intrauterine growth restriction leads to a dysregulation of Wilms' tumour supressor gene 1 (WT1) and to early podocyte alterations.

report of inhibition of FSHRexpression byWT1in ovarian tissue, and inhibition probably occurs via direct interaction with the FSHR promoter
Title: Regulation of FSH receptor expression by the Wilms' tumor 1 gene product (WT1) in immature rat granulosa cells.

The re-expression of WT1 and Pax2 in the tubular epithelial cells plays important roles in the promotion of epithelial-mesenchymal transition.
Title: WT1 and Pax2 re-expression is required for epithelial-mesenchymal transition in 5/6 nephrectomized rats and cultured kidney tubular epithelial cells.

ATRA increases the number of glomerular epithelial transition cells in experimental proteinuric glomerular diseases. Thus, ATRA may provide a useful pharmacologic approach to decipher the
Title: Retinoids augment the expression of podocyte proteins by glomerular parietal epithelial cells in experimental glomerular disease.

The following Wt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12224	NM_031534.2 Latest version!	Rattus norvegicus WT1 transcription factor (Wt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
ORa26129	XM_006234620.1 Latest version!	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa26130	XM_006234621.3 Latest version!	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa26131	XM_006234622.3 Latest version!	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa60267	XM_017591476.1 Latest version!	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa60268	XM_017591477.1 Latest version!	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wt1 ( NM_031534.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113722.2 Rattus norvegicus WT1 transcription factor (Wt1), mRNA.

Price & Availability↑

CloneID	ORa12224
Clone ID Related Accession (Same CDS sequence)	NM_031534.2
Accession Version	NM_031534.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Rattus norvegicus(Norway rat)
Product	Wilms tumor protein homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X69716.1, AABR07053172.1, CO382418.1, AABR07053173.1 and AA899753.1. On Jul 24, 2007 this sequence version replaced NM_031534.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X69716.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## undergoes RNA editing :: PMID: 7926762, 12045112 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGAATGGCT GCTGGGAGCT CCAGCTCAGT GAAATGGACA 
GAAGGGCAGA GCAACCACGG CACAGGGTAC GAGAGTGAGA ACCACACAAC CCCCATCCTC 
TGTGGTGCCC AGTACAGAAT ACACACCCAC GGTGTCTTCC GAGGCATTCA GGATGTGCGG 
CGCGTATCTG GAGTGGCCCC AACTCTTGTC CGGTCAGCAT CTGAAACCAG TGAGAAGCGT 
CCTTTCATGT GTGCGTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCC GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGCGAG 
AGAAGGTTTT CTCGCTCAGA CCAGCTCAAA AGGCACCAAA GGAGACACAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTTTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCACAGTTGT 
CAGAAAAAGT TTGCGCGGTC AGACGAATTA GTCCGCCATC ACAACATGCA CCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113722.2
CDS	16..1362
Translation	MGSDVRDLNALLPAVSSLGGGGGCGLPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPSIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSNHGTGYESENHTTPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	NM_031534.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus WT1 transcription factor (Wt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031534.2

ORF Insert Sequence:

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGAATGGCT GCTGGGAGCT CCAGCTCAGT GAAATGGACA 
GAAGGGCAGA GCAACCACGG CACAGGGTAC GAGAGTGAGA ACCACACAAC CCCCATCCTC 
TGTGGTGCCC AGTACAGAAT ACACACCCAC GGTGTCTTCC GAGGCATTCA GGATGTGCGG 
CGCGTATCTG GAGTGGCCCC AACTCTTGTC CGGTCAGCAT CTGAAACCAG TGAGAAGCGT 
CCTTTCATGT GTGCGTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCC GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGCGAG 
AGAAGGTTTT CTCGCTCAGA CCAGCTCAAA AGGCACCAAA GGAGACACAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTTTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCACAGTTGT 
CAGAAAAAGT TTGCGCGGTC AGACGAATTA GTCCGCCATC ACAACATGCA CCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wt1 ( XM_006234620.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234682.1 Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26129
Clone ID Related Accession (Same CDS sequence)	XM_006234620.1
Accession Version	XM_006234620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Wilms tumor protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGCCACGGC ACAGGGTACG AGAGTGAGAA CCACACAACC 
CCCATCCTCT GTGGTGCCCA GTACAGAATA CACACCCACG GTGTCTTCCG AGGCATTCAG 
GATGTGCGGC GCGTATCTGG AGTGGCCCCA ACTCTTGTCC GGTCAGCATC TGAAACCAGT 
GAGAAGCGTC CTTTCATGTG TGCGTACCCA GGCTGCAATA AGAGATATTT TAAGCTGTCC 
CACTTACAGA TGCACAGCCG GAAGCACACT GGTGAGAAAC CATACCAGTG TGACTTCAAG 
GACTGCGAGA GAAGGTTTTC TCGCTCAGAC CAGCTCAAAA GGCACCAAAG GAGACACACA 
GGTGTGAAAC CATTCCAGTG TAAAACTTGT CAGCGAAAGT TTTCCCGGTC CGACCACCTG 
AAGACCCACA CCAGGACTCA TACAGGTAAA ACAAGTGAAA AGCCCTTCAG CTGTCGGTGG 
CACAGTTGTC AGAAAAAGTT TGCGCGGTCA GACGAATTAG TCCGCCATCA CAACATGCAC 
CAGAGAAACA TGACCAAACT CCAGCTGGCG CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234682.1
CDS	409..1704
Translation	MGSDVRDLNALLPAVSSLGGGGGCGLPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPSIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHGTGYESENHTTPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	XM_006234620.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234620.1

ORF Insert Sequence:

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGCCACGGC ACAGGGTACG AGAGTGAGAA CCACACAACC 
CCCATCCTCT GTGGTGCCCA GTACAGAATA CACACCCACG GTGTCTTCCG AGGCATTCAG 
GATGTGCGGC GCGTATCTGG AGTGGCCCCA ACTCTTGTCC GGTCAGCATC TGAAACCAGT 
GAGAAGCGTC CTTTCATGTG TGCGTACCCA GGCTGCAATA AGAGATATTT TAAGCTGTCC 
CACTTACAGA TGCACAGCCG GAAGCACACT GGTGAGAAAC CATACCAGTG TGACTTCAAG 
GACTGCGAGA GAAGGTTTTC TCGCTCAGAC CAGCTCAAAA GGCACCAAAG GAGACACACA 
GGTGTGAAAC CATTCCAGTG TAAAACTTGT CAGCGAAAGT TTTCCCGGTC CGACCACCTG 
AAGACCCACA CCAGGACTCA TACAGGTAAA ACAAGTGAAA AGCCCTTCAG CTGTCGGTGG 
CACAGTTGTC AGAAAAAGTT TGCGCGGTCA GACGAATTAG TCCGCCATCA CAACATGCAC 
CAGAGAAACA TGACCAAACT CCAGCTGGCG CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wt1 ( XM_006234621.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234683.1 Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa26130
Clone ID Related Accession (Same CDS sequence)	XM_006234621.3
Accession Version	XM_006234621.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Wilms tumor protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234621.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGAATGGCT GCTGGGAGCT CCAGCTCAGT GAAATGGACA 
GAAGGGCAGA GCAACCACGG CACAGGGTAC GAGAGTGAGA ACCACACAAC CCCCATCCTC 
TGTGGTGCCC AGTACAGAAT ACACACCCAC GGTGTCTTCC GAGGCATTCA GGATGTGCGG 
CGCGTATCTG GAGTGGCCCC AACTCTTGTC CGGTCAGCAT CTGAAACCAG TGAGAAGCGT 
CCTTTCATGT GTGCGTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCC GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGCGAG 
AGAAGGTTTT CTCGCTCAGA CCAGCTCAAA AGGCACCAAA GGAGACACAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTTTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTGA AAAGCCCTTC AGCTGTCGGT GGCACAGTTG TCAGAAAAAG 
TTTGCGCGGT CAGACGAATT AGTCCGCCAT CACAACATGC ACCAGAGAAA CATGACCAAA 
CTCCAGCTGG CGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234683.1
CDS	507..1844
Translation	MGSDVRDLNALLPAVSSLGGGGGCGLPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPSIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSNHGTGYESENHTTPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	XM_006234621.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234621.3

ORF Insert Sequence:

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGAATGGCT GCTGGGAGCT CCAGCTCAGT GAAATGGACA 
GAAGGGCAGA GCAACCACGG CACAGGGTAC GAGAGTGAGA ACCACACAAC CCCCATCCTC 
TGTGGTGCCC AGTACAGAAT ACACACCCAC GGTGTCTTCC GAGGCATTCA GGATGTGCGG 
CGCGTATCTG GAGTGGCCCC AACTCTTGTC CGGTCAGCAT CTGAAACCAG TGAGAAGCGT 
CCTTTCATGT GTGCGTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCC GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGCGAG 
AGAAGGTTTT CTCGCTCAGA CCAGCTCAAA AGGCACCAAA GGAGACACAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTTTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTGA AAAGCCCTTC AGCTGTCGGT GGCACAGTTG TCAGAAAAAG 
TTTGCGCGGT CAGACGAATT AGTCCGCCAT CACAACATGC ACCAGAGAAA CATGACCAAA 
CTCCAGCTGG CGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wt1 ( XM_006234622.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234684.1 Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X3, mRNA.

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CloneID	ORa26131
Clone ID Related Accession (Same CDS sequence)	XM_006234622.3
Accession Version	XM_006234622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Wilms tumor protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGCCACGGC ACAGGGTACG AGAGTGAGAA CCACACAACC 
CCCATCCTCT GTGGTGCCCA GTACAGAATA CACACCCACG GTGTCTTCCG AGGCATTCAG 
GATGTGCGGC GCGTATCTGG AGTGGCCCCA ACTCTTGTCC GGTCAGCATC TGAAACCAGT 
GAGAAGCGTC CTTTCATGTG TGCGTACCCA GGCTGCAATA AGAGATATTT TAAGCTGTCC 
CACTTACAGA TGCACAGCCG GAAGCACACT GGTGAGAAAC CATACCAGTG TGACTTCAAG 
GACTGCGAGA GAAGGTTTTC TCGCTCAGAC CAGCTCAAAA GGCACCAAAG GAGACACACA 
GGTGTGAAAC CATTCCAGTG TAAAACTTGT CAGCGAAAGT TTTCCCGGTC CGACCACCTG 
AAGACCCACA CCAGGACTCA TACAGGTGAA AAGCCCTTCA GCTGTCGGTG GCACAGTTGT 
CAGAAAAAGT TTGCGCGGTC AGACGAATTA GTCCGCCATC ACAACATGCA CCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234684.1
CDS	508..1794
Translation	MGSDVRDLNALLPAVSSLGGGGGCGLPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPSIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHGTGYESENHTTPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	XM_006234622.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234622.3

ORF Insert Sequence:

ATGGGTTCCG ACGTGCGGGA CCTGAACGCG CTGCTGCCCG CCGTGTCCTC GCTGGGCGGT 
GGAGGCGGCT GTGGGCTCCC TGTGAGCGGC GCGGCGCAAT GGGCGCCGGT GCTGGACTTC 
GCGCCTCCGG GCGCCTCGGC TTACGGGTCA CTGGGCGGTC CCGCGCCTCC TCCCGCTCCG 
CCGCCACCTC CGCCGCCACC CCACTCCTTC ATCAAACAGG AGCCCAGCTG GGGCGGCGCC 
GAGCCGCACG AGGAGCAGTG CCTGAGCGCC TTCACCTTGC ACTTCTCCGG CCAGTTCACC 
GGTACAGCCG GGGCCTGTCG CTACGGACCC TTCGGTCCTC CCCCGCCCAG CCAGGCGTCA 
TCCGGCCAGG CCAGGATGTT CCCCAATGCG CCCTACCTGC CCAGCTGCCT GGAGAGCCAG 
CCCTCCATCC GCAACCAAGG ATACAGCACG GTCACTTTCG ACGGGGCACC CAGTTATGGC 
CACACGCCCT CGCACCATGC AGCGCAGTTC CCCAATCATT CCTTCAAACA CGAGGACCCC 
ATGGGCCAGC AGGGCTCGCT GGGCGAGCAG CAGTACTCGG TGCCACCTCC AGTGTATGGC 
TGCCACACCC CTACCGACAG TTGCACAGGC AGCCAGGCCC TGCTCCTGAG GACGCCCTAC 
AGCAGTGACA ATTTATACCA GATGACCTCC CAGCTTGAGT GCATGACCTG GAACCAGATG 
AACCTCGGAG CTACCTTGAA GGGCCACGGC ACAGGGTACG AGAGTGAGAA CCACACAACC 
CCCATCCTCT GTGGTGCCCA GTACAGAATA CACACCCACG GTGTCTTCCG AGGCATTCAG 
GATGTGCGGC GCGTATCTGG AGTGGCCCCA ACTCTTGTCC GGTCAGCATC TGAAACCAGT 
GAGAAGCGTC CTTTCATGTG TGCGTACCCA GGCTGCAATA AGAGATATTT TAAGCTGTCC 
CACTTACAGA TGCACAGCCG GAAGCACACT GGTGAGAAAC CATACCAGTG TGACTTCAAG 
GACTGCGAGA GAAGGTTTTC TCGCTCAGAC CAGCTCAAAA GGCACCAAAG GAGACACACA 
GGTGTGAAAC CATTCCAGTG TAAAACTTGT CAGCGAAAGT TTTCCCGGTC CGACCACCTG 
AAGACCCACA CCAGGACTCA TACAGGTGAA AAGCCCTTCA GCTGTCGGTG GCACAGTTGT 
CAGAAAAAGT TTGCGCGGTC AGACGAATTA GTCCGCCATC ACAACATGCA CCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wt1 ( XM_017591476.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446965.1 Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X4, mRNA.

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CloneID	ORa60267
Clone ID Related Accession (Same CDS sequence)	XM_017591476.1
Accession Version	XM_017591476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Wilms tumor protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGG GATACAGCAC GGTCACTTTC GACGGGGCAC CCAGTTATGG CCACACGCCC 
TCGCACCATG CAGCGCAGTT CCCCAATCAT TCCTTCAAAC ACGAGGACCC CATGGGCCAG 
CAGGGCTCGC TGGGCGAGCA GCAGTACTCG GTGCCACCTC CAGTGTATGG CTGCCACACC 
CCTACCGACA GTTGCACAGG CAGCCAGGCC CTGCTCCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AGATGACCTC CCAGCTTGAG TGCATGACCT GGAACCAGAT GAACCTCGGA 
GCTACCTTGA AGGGAATGGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG 
AGCAACCACG GCACAGGGTA CGAGAGTGAG AACCACACAA CCCCCATCCT CTGTGGTGCC 
CAGTACAGAA TACACACCCA CGGTGTCTTC CGAGGCATTC AGGATGTGCG GCGCGTATCT 
GGAGTGGCCC CAACTCTTGT CCGGTCAGCA TCTGAAACCA GTGAGAAGCG TCCTTTCATG 
TGTGCGTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
CGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGCGA GAGAAGGTTT 
TCTCGCTCAG ACCAGCTCAA AAGGCACCAA AGGAGACACA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTTTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCACAGTT GTCAGAAAAA GTTTGCGCGG 
TCAGACGAAT TAGTCCGCCA TCACAACATG CACCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446965.1
CDS	3636..4544
Translation	MEKGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSNHGTGYESENHTTPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	XM_017591476.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591476.1

ORF Insert Sequence:

ATGGAGAAGG GATACAGCAC GGTCACTTTC GACGGGGCAC CCAGTTATGG CCACACGCCC 
TCGCACCATG CAGCGCAGTT CCCCAATCAT TCCTTCAAAC ACGAGGACCC CATGGGCCAG 
CAGGGCTCGC TGGGCGAGCA GCAGTACTCG GTGCCACCTC CAGTGTATGG CTGCCACACC 
CCTACCGACA GTTGCACAGG CAGCCAGGCC CTGCTCCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AGATGACCTC CCAGCTTGAG TGCATGACCT GGAACCAGAT GAACCTCGGA 
GCTACCTTGA AGGGAATGGC TGCTGGGAGC TCCAGCTCAG TGAAATGGAC AGAAGGGCAG 
AGCAACCACG GCACAGGGTA CGAGAGTGAG AACCACACAA CCCCCATCCT CTGTGGTGCC 
CAGTACAGAA TACACACCCA CGGTGTCTTC CGAGGCATTC AGGATGTGCG GCGCGTATCT 
GGAGTGGCCC CAACTCTTGT CCGGTCAGCA TCTGAAACCA GTGAGAAGCG TCCTTTCATG 
TGTGCGTACC CAGGCTGCAA TAAGAGATAT TTTAAGCTGT CCCACTTACA GATGCACAGC 
CGGAAGCACA CTGGTGAGAA ACCATACCAG TGTGACTTCA AGGACTGCGA GAGAAGGTTT 
TCTCGCTCAG ACCAGCTCAA AAGGCACCAA AGGAGACACA CAGGTGTGAA ACCATTCCAG 
TGTAAAACTT GTCAGCGAAA GTTTTCCCGG TCCGACCACC TGAAGACCCA CACCAGGACT 
CATACAGGTG AAAAGCCCTT CAGCTGTCGG TGGCACAGTT GTCAGAAAAA GTTTGCGCGG 
TCAGACGAAT TAGTCCGCCA TCACAACATG CACCAGAGAA ACATGACCAA ACTCCAGCTG 
GCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wt1 ( XM_017591477.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446966.1 Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa60268
Clone ID Related Accession (Same CDS sequence)	XM_017591477.1
Accession Version	XM_017591477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	Wilms tumor protein homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGG GATACAGCAC GGTCACTTTC GACGGGGCAC CCAGTTATGG CCACACGCCC 
TCGCACCATG CAGCGCAGTT CCCCAATCAT TCCTTCAAAC ACGAGGACCC CATGGGCCAG 
CAGGGCTCGC TGGGCGAGCA GCAGTACTCG GTGCCACCTC CAGTGTATGG CTGCCACACC 
CCTACCGACA GTTGCACAGG CAGCCAGGCC CTGCTCCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AGATGACCTC CCAGCTTGAG TGCATGACCT GGAACCAGAT GAACCTCGGA 
GCTACCTTGA AGGGCCACGG CACAGGGTAC GAGAGTGAGA ACCACACAAC CCCCATCCTC 
TGTGGTGCCC AGTACAGAAT ACACACCCAC GGTGTCTTCC GAGGCATTCA GGATGTGCGG 
CGCGTATCTG GAGTGGCCCC AACTCTTGTC CGGTCAGCAT CTGAAACCAG TGAGAAGCGT 
CCTTTCATGT GTGCGTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCC GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGCGAG 
AGAAGGTTTT CTCGCTCAGA CCAGCTCAAA AGGCACCAAA GGAGACACAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTTTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCACAGTTGT 
CAGAAAAAGT TTGCGCGGTC AGACGAATTA GTCCGCCATC ACAACATGCA CCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446966.1
CDS	3636..4502
Translation	MEKGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHGTGYESENHTTPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLQLAL

Target ORF information:

RefSeq Version	XM_017591477.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Wilms tumor 1 (Wt1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591477.1

ORF Insert Sequence:

ATGGAGAAGG GATACAGCAC GGTCACTTTC GACGGGGCAC CCAGTTATGG CCACACGCCC 
TCGCACCATG CAGCGCAGTT CCCCAATCAT TCCTTCAAAC ACGAGGACCC CATGGGCCAG 
CAGGGCTCGC TGGGCGAGCA GCAGTACTCG GTGCCACCTC CAGTGTATGG CTGCCACACC 
CCTACCGACA GTTGCACAGG CAGCCAGGCC CTGCTCCTGA GGACGCCCTA CAGCAGTGAC 
AATTTATACC AGATGACCTC CCAGCTTGAG TGCATGACCT GGAACCAGAT GAACCTCGGA 
GCTACCTTGA AGGGCCACGG CACAGGGTAC GAGAGTGAGA ACCACACAAC CCCCATCCTC 
TGTGGTGCCC AGTACAGAAT ACACACCCAC GGTGTCTTCC GAGGCATTCA GGATGTGCGG 
CGCGTATCTG GAGTGGCCCC AACTCTTGTC CGGTCAGCAT CTGAAACCAG TGAGAAGCGT 
CCTTTCATGT GTGCGTACCC AGGCTGCAAT AAGAGATATT TTAAGCTGTC CCACTTACAG 
ATGCACAGCC GGAAGCACAC TGGTGAGAAA CCATACCAGT GTGACTTCAA GGACTGCGAG 
AGAAGGTTTT CTCGCTCAGA CCAGCTCAAA AGGCACCAAA GGAGACACAC AGGTGTGAAA 
CCATTCCAGT GTAAAACTTG TCAGCGAAAG TTTTCCCGGT CCGACCACCT GAAGACCCAC 
ACCAGGACTC ATACAGGTAA AACAAGTGAA AAGCCCTTCA GCTGTCGGTG GCACAGTTGT 
CAGAAAAAGT TTGCGCGGTC AGACGAATTA GTCCGCCATC ACAACATGCA CCAGAGAAAC 
ATGACCAAAC TCCAGCTGGC GCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WT1 ameliorates podocyte injury via repression of EZH2/?-catenin pathway in diabetic nephropathy.
Free radical biology & medicine108280-299(2017 Jul)
Wan J,Hou X,Zhou Z,Geng J,Tian J,Bai X,Nie J

Changes in renal WT-1 expression preceding hypertension development.
BMC nephrology1734(2016 Mar)
Mazzei L,Garc?a M,Calvo JP,Casarotto M,Forn?s M,Abud MA,Cuello-Carri?n D,Ferder L,Manucha W

Wilms tumor protein recognizes 5-carboxylcytosine within a specific DNA sequence.
Genes & development28(20)2304-13(2014 Oct)
Hashimoto H,Olanrewaju YO,Zheng Y,Wilson GG,Zhang X,Cheng X

Intrauterine growth restriction leads to a dysregulation of Wilms' tumour supressor gene 1 (WT1) and to early podocyte alterations.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association28(6)1407-17(2013 Jun)
Menendez-Castro C,Hilgers KF,Amann K,Daniel C,Cordasic N,Wachtveitl R,Fahlbusch F,Plank C,D?tsch J,Rascher W,Hartner A

Retinoids augment the expression of podocyte proteins by glomerular parietal epithelial cells in experimental glomerular disease.
Nephron. Experimental nephrology121(1-2)e23-37(2012)
Zhang J,Pippin JW,Vaughan MR,Krofft RD,Taniguchi Y,Romagnani P,Nelson PJ,Liu ZH,Shankland SJ

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Wt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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