Ucp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ucp1
Entrez Gene ID	24860
Full Name	uncoupling protein 1
Synonyms	Ucp,Ucpa,Uncp
General protein information	Preferred Names uncoupling protein 1 Names mitochondrial brown fat uncoupling protein 1 UCP 1 solute carrier family 25 member 7 thermogenin uncoupling protein 1 (mitochondrial, proton carrier)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	plays a role in heat production by uncoupling oxidative phosphorylation from the respiratory chain [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012682.2	NP_036814.1	mitochondrial brown fat uncoupling protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UCP1	XP_517450.3
Bos taurus (cattle)	UCP1	NP_001160000.1
Rattus norvegicus (Norway rat)	Ucp1	NP_036814.1
	UCP1	XP_001090457.1
Homo sapiens (human)	UCP1	NP_068605.1
Canis lupus familiaris (dog)	UCP1	NP_001003046.1
Mus musculus (house mouse)	Ucp1	NP_033489.1

Gene Ontology
Bibliography

Related articles in PubMed

UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction.
Kazak L, Chouchani ET, Stavrovskaya IG, Lu GZ, Jedrychowski MP, Egan DF, Kumari M, Kong X, Erickson BK, Szpyt J, Rosen ED, Murphy MP, Kristal BS, Gygi SP, Spiegelman BM
Proceedings of the National Academy of Sciences of the United States of America114(30)7981-7986(2017 Jul)

UCP1, the mitochondrial uncoupling protein of brown adipocyte: A?personal contribution and a historical perspective.
Ricquier D
Biochimie1343-8(2017 Mar)

The distinct bioenergetic properties of the human UCP1.
Rodr?guez-S?nchez L, Rial E
Biochimie13451-55(2017 Mar)

Rapamycin negatively impacts insulin signaling, glucose uptake and uncoupling protein-1 in brown adipocytes.
Garc?a-Casarrubios E, de Moura C, Arroba AI, Pescador N, Calderon-Dominguez M, Garcia L, Herrero L, Serra D, Cadenas S, Reis F, Carvalho E, Obregon MJ, Valverde ?M
Biochimica et biophysica acta1861(12 Pt A)1929-1941(2016 12)

Continuous cold exposure induces an anti-inflammatory response in mesenteric adipose tissue associated with catecholamine production and thermogenin expression in rats.
Vargovic P, Manz G, Kvetnansky R
Endocrine regulations50(3)137-44(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The history of discovery of UCP1, the mitochondrial uncoupling protein of brown adipocyte, has been described. (Review)
Title: UCP1, the mitochondrial uncoupling protein of brown adipocyte: A?personal contribution and a historical perspective.

In the absence of regulators (fatty acids, retinoids), rodent UCP1 presents a high ohmic proton conductance that cannot be detected in human UCP1.
Title: The distinct bioenergetic properties of the human UCP1.

The expression of peroxisome proliferator-activated receptor gamma coactivator 1alpha (PGC-1alpha) and uncoupling protein (UCP)-1 in brown adipocytes.
Title: Rapamycin negatively impacts insulin signaling, glucose uptake and uncoupling protein-1 in brown adipocytes.

Data indicate that prolonged cold exposure may induce anti-inflammatory response in mesenteric white adipose tissue associated with induction of UCP-1 expression.
Title: Continuous cold exposure induces an anti-inflammatory response in mesenteric adipose tissue associated with catecholamine production and thermogenin expression in rats.

e found that CPT1AM-expressing rBA have increased FAO, lipolysis, UCP1 protein levels and mitochondrial activity. Additionally, enhanced FAO reduced the palmitate-induced increase in triglyceride content and the expression of obese and inflammatory markers. Thus, CPT1AM-expressing rBA had enhanced fat-burning capacity and improved lipid-induced derangements.
Title: Carnitine Palmitoyltransferase 1 Increases Lipolysis, UCP1 Protein Expression and Mitochondrial Activity in Brown Adipocytes.

The following Ucp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ucp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12208	NM_012682.2 Latest version!	Rattus norvegicus uncoupling protein 1 (Ucp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ucp1 ( NM_012682.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036814.1 Rattus norvegicus uncoupling protein 1 (Ucp1), mRNA.

Price & Availability↑

CloneID	ORa12208
Clone ID Related Accession (Same CDS sequence)	NM_012682.2
Accession Version	NM_012682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial brown fat uncoupling protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FM087156.1 and BC088156.1. On Aug 18, 2012 this sequence version replaced NM_012682.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088156.1, M11814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689605 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGTGAGTT CGACAACTTC CGAAGTGCAA CCCACCATGG GGGTCAAGAT CTTCTCAGCC 
GGCGTTTCTG CCTGCCTAGC AGACATCATC ACCTTCCCGC TGGACACCGC CAAAGTCCGC 
CTTCAGATCC AAGGTGAAGG CCAGGCTTCC AGTACTATTA GGTATAAAGG TGTCTTAGGG 
ACCATCACCA CCCTGGCCAA GACAGAAGGA TTGCCGAAAC TGTACAGCGG TCTGCCTGCT 
GGCATCCAGA GGCAAATCAG CTTTGCTTCC CTCAGGATTG GCCTCTACGA TACGGTCCAA 
GAGTACTTCT CTTCAGGGAG AGAAACGCCT GCCTCTTTGG GAAGCAAGAT CTCGGCTGGC 
TTGATGACGG GTGGCGTGGC GGTATTCATT GGGCAGCCCA CAGAGGTGGT GAAGGTCAGA 
ATGCAAGCAC AAAGCCATCT GCACGGGATC AAACCCCGCT ACACTGGGAC CTACAATGCT 
TACAGAGTTA TAGCCACCAC AGAAAGCTTG TCAACACTGT GGAAAGGGAC GACTCCTAAT 
CTAATGAGAA ATGTCATCAT CAACTGTACA GAGCTGGTGA CATATGACCT CATGAAGGGG 
GCCCTTGTGA ACCACCACAT ACTGGCAGAT GACGTCCCCT GCCATTTACT GTCAGCTCTT 
GTCGCCGGGT TTTGCACCAC ACTCCTGGCC TCTCCGGTGG ATGTGGTAAA AACGAGATTC 
ATCAACTCTC TACCAGGACA GTACCCAAGT GTACCCAGCT GTGCAATGAC CATGTACACC 
AAGGAAGGAC CGGCAGCCTT TTTCAAAGGG TTTGCGCCTT CTTTTCTGCG ACTCGGATCC 
TGGAACGTCA TCATGTTTGT GTGCTTTGAA CAGCTGAAGA AAGAGCTGAT GAAGTCCCGG 
CAGACAGTGG ACTGCACCAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036814.1
CDS	182..1105
Translation	MVSSTTSEVQPTMGVKIFSAGVSACLADIITFPLDTAKVRLQIQGEGQASSTIRYKGVLGTITTLAKTEGLPKLYSGLPAGIQRQISFASLRIGLYDTVQEYFSSGRETPASLGSKISAGLMTGGVAVFIGQPTEVVKVRMQAQSHLHGIKPRYTGTYNAYRVIATTESLSTLWKGTTPNLMRNVIINCTELVTYDLMKGALVNHHILADDVPCHLLSALVAGFCTTLLASPVDVVKTRFINSLPGQYPSVPSCAMTMYTKEGPAAFFKGFAPSFLRLGSWNVIMFVCFEQLKKELMKSRQTVDCTT

Target ORF information:

RefSeq Version	NM_012682.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus uncoupling protein 1 (Ucp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012682.2

ORF Insert Sequence:

ATGGTGAGTT CGACAACTTC CGAAGTGCAA CCCACCATGG GGGTCAAGAT CTTCTCAGCC 
GGCGTTTCTG CCTGCCTAGC AGACATCATC ACCTTCCCGC TGGACACCGC CAAAGTCCGC 
CTTCAGATCC AAGGTGAAGG CCAGGCTTCC AGTACTATTA GGTATAAAGG TGTCTTAGGG 
ACCATCACCA CCCTGGCCAA GACAGAAGGA TTGCCGAAAC TGTACAGCGG TCTGCCTGCT 
GGCATCCAGA GGCAAATCAG CTTTGCTTCC CTCAGGATTG GCCTCTACGA TACGGTCCAA 
GAGTACTTCT CTTCAGGGAG AGAAACGCCT GCCTCTTTGG GAAGCAAGAT CTCGGCTGGC 
TTGATGACGG GTGGCGTGGC GGTATTCATT GGGCAGCCCA CAGAGGTGGT GAAGGTCAGA 
ATGCAAGCAC AAAGCCATCT GCACGGGATC AAACCCCGCT ACACTGGGAC CTACAATGCT 
TACAGAGTTA TAGCCACCAC AGAAAGCTTG TCAACACTGT GGAAAGGGAC GACTCCTAAT 
CTAATGAGAA ATGTCATCAT CAACTGTACA GAGCTGGTGA CATATGACCT CATGAAGGGG 
GCCCTTGTGA ACCACCACAT ACTGGCAGAT GACGTCCCCT GCCATTTACT GTCAGCTCTT 
GTCGCCGGGT TTTGCACCAC ACTCCTGGCC TCTCCGGTGG ATGTGGTAAA AACGAGATTC 
ATCAACTCTC TACCAGGACA GTACCCAAGT GTACCCAGCT GTGCAATGAC CATGTACACC 
AAGGAAGGAC CGGCAGCCTT TTTCAAAGGG TTTGCGCCTT CTTTTCTGCG ACTCGGATCC 
TGGAACGTCA TCATGTTTGT GTGCTTTGAA CAGCTGAAGA AAGAGCTGAT GAAGTCCCGG 
CAGACAGTGG ACTGCACCAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

UCP1 deficiency causes brown fat respiratory chain depletion and sensitizes mitochondria to calcium overload-induced dysfunction.
Proceedings of the National Academy of Sciences of the United States of America114(30)7981-7986(2017 Jul)
Kazak L,Chouchani ET,Stavrovskaya IG,Lu GZ,Jedrychowski MP,Egan DF,Kumari M,Kong X,Erickson BK,Szpyt J,Rosen ED,Murphy MP,Kristal BS,Gygi SP,Spiegelman BM

UCP1, the mitochondrial uncoupling protein of brown adipocyte: A?personal contribution and a historical perspective.
Biochimie1343-8(2017 Mar)
Ricquier D

The distinct bioenergetic properties of the human UCP1.
Biochimie13451-55(2017 Mar)
Rodr?guez-S?nchez L,Rial E

Rapamycin negatively impacts insulin signaling, glucose uptake and uncoupling protein-1 in brown adipocytes.
Biochimica et biophysica acta1861(12 Pt A)1929-1941(2016 12)
Garc?a-Casarrubios E,de Moura C,Arroba AI,Pescador N,Calderon-Dominguez M,Garcia L,Herrero L,Serra D,Cadenas S,Reis F,Carvalho E,Obregon MJ,Valverde ?M

Continuous cold exposure induces an anti-inflammatory response in mesenteric adipose tissue associated with catecholamine production and thermogenin expression in rats.
Endocrine regulations50(3)137-44(2016 Jul)
Vargovic P,Manz G,Kvetnansky R

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Ucp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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