Raf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Raf1
Entrez Gene ID	24703
Full Name	Raf-1 proto-oncogene, serine/threonine kinase
General protein information	Preferred Names Raf-1 proto-oncogene, serine/threonine kinase Names RAF proto-oncogene serine/threonine-protein kinase C-RAF cRaf murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV) proto-oncogene c-RAF v-raf-1 murine leukemia viral oncogene homolog 1 v-raf-leukemia viral oncogene 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q42
Summary	acts as a mitogenic protein kinase; mutant forms may play a role in transformation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763217.1	XP_008761439.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
XM_008763218.2	XP_008761440.1	RAF proto-oncogene serine/threonine-protein kinase isoform X1
NM_012639.2	NP_036771.1	RAF proto-oncogene serine/threonine-protein kinase

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	raf1	NP_001017117.1
Homo sapiens (human)	RAF1	NP_002871.1
Canis lupus familiaris (dog)	RAF1	XP_005632198.1
Mus musculus (house mouse)	Raf1	NP_084056.1
	RAF1	XP_001086154.1
Rattus norvegicus (Norway rat)	Raf1	NP_036771.1
Gallus gallus (chicken)	RAF1	NP_990638.1
Pan troglodytes (chimpanzee)	RAF1	XP_516289.2
Danio rerio (zebrafish)	raf1b	NP_001186684.1

Gene Ontology
Bibliography

Related articles in PubMed

Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.
Kamemura N, Murakami S, Komatsu H, Sawanoi M, Miyamoto K, Ishidoh K, Kishimoto K, Tsuji A, Yuasa K
Biochemical and biophysical research communications483(1)82-87(2017 01)

Possible Role of Raf-1 Kinase in the Development of Cerebral Vasospasm and Early Brain Injury After Experimental Subarachnoid Hemorrhage in Rats.
Zhang J, Xu X, Zhou D, Li H, You W, Wang Z, Chen G
Molecular neurobiology52(3)1527-1539(2015 Dec)

Multikinase inhibitor sorafenib prevents pressure overload-induced left ventricular hypertrophy in rats by blocking the c-Raf/ERK1/2 signaling pathway.
Daryadel A, Bogdanova A, Gassmann M, Mueller X, Z?nd G, Seifert B, Lehalle C, Frossard N, Tavakoli R
Journal of cardiothoracic surgery981(2014 May)

Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras-Raf-ERK-Sp1 signaling axis in C6 glioma cells.
Chang KW, Huang YL, Wong ZR, Su PH, Huang BM, Ju TK, Yang HY
Biochemical and biophysical research communications434(4)854-60(2013 May)

X-linked inhibitor of apoptosis protein negatively regulates neuronal differentiation through interaction with cRAF and Trk.
Fad? R, Moubarak RS, Mi?ano-Molina AJ, Barneda-Zahonero B, Valero J, Saura CA, Moran J, Comella JX, Rodr?guez-?lvarez J
Scientific reports32397(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not.
Title: Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.

Raf-1 kinase contributes to cerebral vasospasm and early brain injury after subarachnoid hemorrhage by enhancing the activation of the Raf-1/ERK1/2 and Raf-1/NF-kappaB signaling pathways.
Title: Possible Role of Raf-1 Kinase in the Development of Cerebral Vasospasm and Early Brain Injury After Experimental Subarachnoid Hemorrhage in Rats.

sorafenib exhibits a regulatory role on the occurrence of LVH following AB in rats by blocking the rise in growth factors PDGF-BB and TGFbeta1, activation of the corresponding c-Raf-ERK1/2 signaling pathway
Title: Multikinase inhibitor sorafenib prevents pressure overload-induced left ventricular hypertrophy in rats by blocking the c-Raf/ERK1/2 signaling pathway.

Data indicate that Ras-Raf-ERK-Sp1 signaling pathway is responsibe for FGF-2-induced nestin expression.
Title: Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras-Raf-ERK-Sp1 signaling axis in C6 glioma cells.

Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons.
Title: X-linked inhibitor of apoptosis protein negatively regulates neuronal differentiation through interaction with cRAF and Trk.

The following Raf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Raf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00521	NM_012639.2 Latest version!	Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$384.30 $549.00
ORa42949	XM_008763217.1 Latest version!	Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa42949	XM_008763218.2 Latest version!	Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Raf1 ( NM_012639.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036771.1 Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), mRNA.

Price & Availability↑

CloneID	ORa00521
Clone ID Related Accession (Same CDS sequence)	NM_012639.2
Accession Version	NM_012639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	RAF proto-oncogene serine/threonine-protein kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC062071.1. On or before Jun 26, 2012 this sequence version replaced XM_003749820.1, NM_012639.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062071.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTCGGACT CAAAGATGCT 
GTGTTTGATG GCTCCAGTTG CATCTCCCCT ACAATTGTTC AGCAGTTTGG CTATCAGCGT 
CGGGCCTCTG ATGATGGCAA ACTCACGGAT TCTTCTAAGA CAAGCAATAC TATCCGGGTT 
TTCTTGCCGA ATAAGCAAAG GACTGTGGTC AATGTGCGGA ATGGGATGAG CTTGCACGAC 
TGCCTTATGA AAGCTCTGAA GGTTAGAGGC CTGCAGCCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTTCAGG AGCACAAAGG TAAGAAAGCA CGCTTAGATT GGAACACCGA CGCCGCCTCT 
CTGATTGGAG AAGAACTGCA AGTGGATTTT TTGGATCACG TTCCACTCAC AACTCACAAC 
TTTGCTCGGA AAACGTTCCT GAAGCTTGCA TTCTGTGACA TCTGTCAAAA GTTCCTGCTA 
AATGGATTTC GATGTCAGAC TTGTGGCTAC AAGTTTCATG AGCACTGTAG CACCAAAGTA 
CCTACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TGCTGTTTCC AAATTCCACT 
GCAAGTGACA GTGGAGTCCC AGCACCACCC TCTTTCACAA TGCGTCGGAT GCGAGAATCT 
GTTTCCCGGA TGCCTGCTAG TTCCCAGCAC AGATACTCCA CACCCCATGC CTTCACTTTC 
AACACCTCCA GCCCTTCCTC TGAAGGTTCC CTGTCCCAGA GGCAGAGGTC AACGTCCACT 
CCCAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGCCTCACCT TCAGCCCTGT CCAGCAGCCC CAACAACCTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGTGCCAG CACAAAGAGA GAGGGCGCCA 
GGATCTGGGA CCCAGGAAAA AAACAAAATT AGGCCTCGTG GGCAGAGAGA TTCAAGTTAT 
TACTGGGAAA TAGAAGCCAG TGAGGTGATG CTGTCTACTC GGATTGGCTC GGGCTCCTTT 
GGCACTGTGT ACAAGGGCAA GTGGCATGGA GATGTTGCAG TAAAGATCCT AAAGGTGGTT 
GACCCAACTC CAGAGCAACT TCAGGCCTTC AGGAACGAGG TGGCTGTTTT GCGCAAAACA 
CGGCATGTTA ATATCCTGCT GTTCATGGGG TACATGACAA AGGACAACCT GGCGATTGTG 
ACCCAGTGGT GTGAAGGCAG CAGTCTCTAC AAACACCTGC ATGTCCAGGA GACCAAATTC 
CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACAGCTC AGGGAATGGA CTATTTACAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCCTC 
ACGGTGAAAA TCGGAGATTT TGGTTTGGCA ACAGTGAAGT CGCGCTGGAG TGGTTCTCAG 
CAGGTTGAAC AGCCCACTGG CTCTGTGCTG TGGATGGCCC CAGAAGTAAT CCGAATGCAG 
GATAACAACC CGTTCAGCTT CCAGTCCGAT GTCTACTCCT ATGGCATTGT GCTGTATGAG 
CTGATGACTG GGGAGCTTCC CTACTCCCAC ATCAACAACC GAGACCAGAT CATCTTCATG 
GTGGGCCGTG GGTACGCCTC CCCAGATCTT AGCAGGCTCT ACAAGAACTG CCCCAAGGCA 
ATGAAGAGGT TGGTGGCTGA CTGTGTGAAG AAAGTCAAAG AAGAAAGGCC TTTGTTTCCT 
CAGATCCTGT CTTCCATTGA GCTGCTTCAG CACTCTCTGC CGAAAATCAA CAGGAGCGCC 
TCTGAGCCTT CCCTGCATCG GGCAGCTCAC ACTGAGGACA TCAATGCTTG TACGCTGACC 
ACATCCCCAA GGCTACCAGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036771.1
CDS	297..2243
Translation	MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTASDSGVPAPPSFTMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	NM_012639.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012639.2

ORF Insert Sequence:

ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTCGGACT CAAAGATGCT 
GTGTTTGATG GCTCCAGTTG CATCTCCCCT ACAATTGTTC AGCAGTTTGG CTATCAGCGT 
CGGGCCTCTG ATGATGGCAA ACTCACGGAT TCTTCTAAGA CAAGCAATAC TATCCGGGTT 
TTCTTGCCGA ATAAGCAAAG GACTGTGGTC AATGTGCGGA ATGGGATGAG CTTGCACGAC 
TGCCTTATGA AAGCTCTGAA GGTTAGAGGC CTGCAGCCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTTCAGG AGCACAAAGG TAAGAAAGCA CGCTTAGATT GGAACACCGA CGCCGCCTCT 
CTGATTGGAG AAGAACTGCA AGTGGATTTT TTGGATCACG TTCCACTCAC AACTCACAAC 
TTTGCTCGGA AAACGTTCCT GAAGCTTGCA TTCTGTGACA TCTGTCAAAA GTTCCTGCTA 
AATGGATTTC GATGTCAGAC TTGTGGCTAC AAGTTTCATG AGCACTGTAG CACCAAAGTA 
CCTACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TGCTGTTTCC AAATTCCACT 
GCAAGTGACA GTGGAGTCCC AGCACCACCC TCTTTCACAA TGCGTCGGAT GCGAGAATCT 
GTTTCCCGGA TGCCTGCTAG TTCCCAGCAC AGATACTCCA CACCCCATGC CTTCACTTTC 
AACACCTCCA GCCCTTCCTC TGAAGGTTCC CTGTCCCAGA GGCAGAGGTC AACGTCCACT 
CCCAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGGATGCA 
ATTCGAAGTC ACAGTGAATC AGCCTCACCT TCAGCCCTGT CCAGCAGCCC CAACAACCTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGTGCCAG CACAAAGAGA GAGGGCGCCA 
GGATCTGGGA CCCAGGAAAA AAACAAAATT AGGCCTCGTG GGCAGAGAGA TTCAAGTTAT 
TACTGGGAAA TAGAAGCCAG TGAGGTGATG CTGTCTACTC GGATTGGCTC GGGCTCCTTT 
GGCACTGTGT ACAAGGGCAA GTGGCATGGA GATGTTGCAG TAAAGATCCT AAAGGTGGTT 
GACCCAACTC CAGAGCAACT TCAGGCCTTC AGGAACGAGG TGGCTGTTTT GCGCAAAACA 
CGGCATGTTA ATATCCTGCT GTTCATGGGG TACATGACAA AGGACAACCT GGCGATTGTG 
ACCCAGTGGT GTGAAGGCAG CAGTCTCTAC AAACACCTGC ATGTCCAGGA GACCAAATTC 
CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACAGCTC AGGGAATGGA CTATTTACAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCCTC 
ACGGTGAAAA TCGGAGATTT TGGTTTGGCA ACAGTGAAGT CGCGCTGGAG TGGTTCTCAG 
CAGGTTGAAC AGCCCACTGG CTCTGTGCTG TGGATGGCCC CAGAAGTAAT CCGAATGCAG 
GATAACAACC CGTTCAGCTT CCAGTCCGAT GTCTACTCCT ATGGCATTGT GCTGTATGAG 
CTGATGACTG GGGAGCTTCC CTACTCCCAC ATCAACAACC GAGACCAGAT CATCTTCATG 
GTGGGCCGTG GGTACGCCTC CCCAGATCTT AGCAGGCTCT ACAAGAACTG CCCCAAGGCA 
ATGAAGAGGT TGGTGGCTGA CTGTGTGAAG AAAGTCAAAG AAGAAAGGCC TTTGTTTCCT 
CAGATCCTGT CTTCCATTGA GCTGCTTCAG CACTCTCTGC CGAAAATCAA CAGGAGCGCC 
TCTGAGCCTT CCCTGCATCG GGCAGCTCAC ACTGAGGACA TCAATGCTTG TACGCTGACC 
ACATCCCCAA GGCTACCAGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Raf1 ( XM_008763217.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761439.1 Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42949
Clone ID Related Accession (Same CDS sequence)	XM_008763217.1 , XM_008763218.2
Accession Version	XM_008763217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTCGGACT CAAAGATGCT 
GTGTTTGATG GCTCCAGTTG CATCTCCCCT ACAATTGTTC AGCAGTTTGG CTATCAGCGT 
CGGGCCTCTG ATGATGGCAA ACTCACGGAT TCTTCTAAGA CAAGCAATAC TATCCGGGTT 
TTCTTGCCGA ATAAGCAAAG GACTGTGGTC AATGTGCGGA ATGGGATGAG CTTGCACGAC 
TGCCTTATGA AAGCTCTGAA GGTTAGAGGC CTGCAGCCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTTCAGG AGCACAAAGG TAAGAAAGCA CGCTTAGATT GGAACACCGA CGCCGCCTCT 
CTGATTGGAG AAGAACTGCA AGTGGATTTT TTGGATCACG TTCCACTCAC AACTCACAAC 
TTTGCTCGGA AAACGTTCCT GAAGCTTGCA TTCTGTGACA TCTGTCAAAA GTTCCTGCTA 
AATGGATTTC GATGTCAGAC TTGTGGCTAC AAGTTTCATG AGCACTGTAG CACCAAAGTA 
CCTACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TGCTGTTTCC AAATTCCACT 
GCAAGTGACA GTGGAGTCCC AGCACCACCC TCTTTCACAA TGCGTCGGAT GCGAGAATCT 
GTTTCCCGGA TGCCTGCTAG TTCCCAGCAC AGATACTCCA CACCCCATGC CTTCACTTTC 
AACACCTCCA GCCCTTCCTC TGAAGGTTCC CTGTCCCAGA GGCAGAGGTC AACGTCCACT 
CCCAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGCTCC AGACGATTTT GTCTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGCCTCACCT TCAGCCCTGT CCAGCAGCCC CAACAACCTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGTGCCAG CACAAAGAGA GAGGGCGCCA 
GGATCTGGGA CCCAGGAAAA AAACAAAATT AGGCCTCGTG GGCAGAGAGA TTCAAGTTAT 
TACTGGGAAA TAGAAGCCAG TGAGGTGATG CTGTCTACTC GGATTGGCTC GGGCTCCTTT 
GGCACTGTGT ACAAGGGCAA GTGGCATGGA GATGTTGCAG TAAAGATCCT AAAGGTGGTT 
GACCCAACTC CAGAGCAACT TCAGGCCTTC AGGAACGAGG TGGCTGTTTT GCGCAAAACA 
CGGCATGTTA ATATCCTGCT GTTCATGGGG TACATGACAA AGGACAACCT GGCGATTGTG 
ACCCAGTGGT GTGAAGGCAG CAGTCTCTAC AAACACCTGC ATGTCCAGGA GACCAAATTC 
CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACAGCTC AGGGAATGGA CTATTTACAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCCTC 
ACGGTGAAAA TCGGAGATTT TGGTTTGGCA ACAGTGAAGT CGCGCTGGAG TGGTTCTCAG 
CAGGTTGAAC AGCCCACTGG CTCTGTGCTG TGGATGGCCC CAGAAGTAAT CCGAATGCAG 
GATAACAACC CGTTCAGCTT CCAGTCCGAT GTCTACTCCT ATGGCATTGT GCTGTATGAG 
CTGATGACTG GGGAGCTTCC CTACTCCCAC ATCAACAACC GAGACCAGAT CATCTTCATG 
GTGGGCCGTG GGTACGCCTC CCCAGATCTT AGCAGGCTCT ACAAGAACTG CCCCAAGGCA 
ATGAAGAGGT TGGTGGCTGA CTGTGTGAAG AAAGTCAAAG AAGAAAGGCC TTTGTTTCCT 
CAGATCCTGT CTTCCATTGA GCTGCTTCAG CACTCTCTGC CGAAAATCAA CAGGAGCGCC 
TCTGAGCCTT CCCTGCATCG GGCAGCTCAC ACTGAGGACA TCAATGCTTG TACGCTGACC 
ACATCCCCAA GGCTACCAGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761439.1
CDS	367..2373
Translation	MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTASDSGVPAPPSFTMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIENNSLNASPRACSRRFCLRGRDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	XM_008763217.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763217.1

ORF Insert Sequence:

ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTCGGACT CAAAGATGCT 
GTGTTTGATG GCTCCAGTTG CATCTCCCCT ACAATTGTTC AGCAGTTTGG CTATCAGCGT 
CGGGCCTCTG ATGATGGCAA ACTCACGGAT TCTTCTAAGA CAAGCAATAC TATCCGGGTT 
TTCTTGCCGA ATAAGCAAAG GACTGTGGTC AATGTGCGGA ATGGGATGAG CTTGCACGAC 
TGCCTTATGA AAGCTCTGAA GGTTAGAGGC CTGCAGCCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTTCAGG AGCACAAAGG TAAGAAAGCA CGCTTAGATT GGAACACCGA CGCCGCCTCT 
CTGATTGGAG AAGAACTGCA AGTGGATTTT TTGGATCACG TTCCACTCAC AACTCACAAC 
TTTGCTCGGA AAACGTTCCT GAAGCTTGCA TTCTGTGACA TCTGTCAAAA GTTCCTGCTA 
AATGGATTTC GATGTCAGAC TTGTGGCTAC AAGTTTCATG AGCACTGTAG CACCAAAGTA 
CCTACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TGCTGTTTCC AAATTCCACT 
GCAAGTGACA GTGGAGTCCC AGCACCACCC TCTTTCACAA TGCGTCGGAT GCGAGAATCT 
GTTTCCCGGA TGCCTGCTAG TTCCCAGCAC AGATACTCCA CACCCCATGC CTTCACTTTC 
AACACCTCCA GCCCTTCCTC TGAAGGTTCC CTGTCCCAGA GGCAGAGGTC AACGTCCACT 
CCCAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGCTCC AGACGATTTT GTCTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGCCTCACCT TCAGCCCTGT CCAGCAGCCC CAACAACCTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGTGCCAG CACAAAGAGA GAGGGCGCCA 
GGATCTGGGA CCCAGGAAAA AAACAAAATT AGGCCTCGTG GGCAGAGAGA TTCAAGTTAT 
TACTGGGAAA TAGAAGCCAG TGAGGTGATG CTGTCTACTC GGATTGGCTC GGGCTCCTTT 
GGCACTGTGT ACAAGGGCAA GTGGCATGGA GATGTTGCAG TAAAGATCCT AAAGGTGGTT 
GACCCAACTC CAGAGCAACT TCAGGCCTTC AGGAACGAGG TGGCTGTTTT GCGCAAAACA 
CGGCATGTTA ATATCCTGCT GTTCATGGGG TACATGACAA AGGACAACCT GGCGATTGTG 
ACCCAGTGGT GTGAAGGCAG CAGTCTCTAC AAACACCTGC ATGTCCAGGA GACCAAATTC 
CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACAGCTC AGGGAATGGA CTATTTACAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCCTC 
ACGGTGAAAA TCGGAGATTT TGGTTTGGCA ACAGTGAAGT CGCGCTGGAG TGGTTCTCAG 
CAGGTTGAAC AGCCCACTGG CTCTGTGCTG TGGATGGCCC CAGAAGTAAT CCGAATGCAG 
GATAACAACC CGTTCAGCTT CCAGTCCGAT GTCTACTCCT ATGGCATTGT GCTGTATGAG 
CTGATGACTG GGGAGCTTCC CTACTCCCAC ATCAACAACC GAGACCAGAT CATCTTCATG 
GTGGGCCGTG GGTACGCCTC CCCAGATCTT AGCAGGCTCT ACAAGAACTG CCCCAAGGCA 
ATGAAGAGGT TGGTGGCTGA CTGTGTGAAG AAAGTCAAAG AAGAAAGGCC TTTGTTTCCT 
CAGATCCTGT CTTCCATTGA GCTGCTTCAG CACTCTCTGC CGAAAATCAA CAGGAGCGCC 
TCTGAGCCTT CCCTGCATCG GGCAGCTCAC ACTGAGGACA TCAATGCTTG TACGCTGACC 
ACATCCCCAA GGCTACCAGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Raf1 ( XM_008763218.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761440.1 Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), transcript variant X2, mRNA.

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CloneID	ORa42949
Clone ID Related Accession (Same CDS sequence)	XM_008763217.1 , XM_008763218.2
Accession Version	XM_008763218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RAF proto-oncogene serine/threonine-protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763218.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTCGGACT CAAAGATGCT 
GTGTTTGATG GCTCCAGTTG CATCTCCCCT ACAATTGTTC AGCAGTTTGG CTATCAGCGT 
CGGGCCTCTG ATGATGGCAA ACTCACGGAT TCTTCTAAGA CAAGCAATAC TATCCGGGTT 
TTCTTGCCGA ATAAGCAAAG GACTGTGGTC AATGTGCGGA ATGGGATGAG CTTGCACGAC 
TGCCTTATGA AAGCTCTGAA GGTTAGAGGC CTGCAGCCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTTCAGG AGCACAAAGG TAAGAAAGCA CGCTTAGATT GGAACACCGA CGCCGCCTCT 
CTGATTGGAG AAGAACTGCA AGTGGATTTT TTGGATCACG TTCCACTCAC AACTCACAAC 
TTTGCTCGGA AAACGTTCCT GAAGCTTGCA TTCTGTGACA TCTGTCAAAA GTTCCTGCTA 
AATGGATTTC GATGTCAGAC TTGTGGCTAC AAGTTTCATG AGCACTGTAG CACCAAAGTA 
CCTACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TGCTGTTTCC AAATTCCACT 
GCAAGTGACA GTGGAGTCCC AGCACCACCC TCTTTCACAA TGCGTCGGAT GCGAGAATCT 
GTTTCCCGGA TGCCTGCTAG TTCCCAGCAC AGATACTCCA CACCCCATGC CTTCACTTTC 
AACACCTCCA GCCCTTCCTC TGAAGGTTCC CTGTCCCAGA GGCAGAGGTC AACGTCCACT 
CCCAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGCTCC AGACGATTTT GTCTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGCCTCACCT TCAGCCCTGT CCAGCAGCCC CAACAACCTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGTGCCAG CACAAAGAGA GAGGGCGCCA 
GGATCTGGGA CCCAGGAAAA AAACAAAATT AGGCCTCGTG GGCAGAGAGA TTCAAGTTAT 
TACTGGGAAA TAGAAGCCAG TGAGGTGATG CTGTCTACTC GGATTGGCTC GGGCTCCTTT 
GGCACTGTGT ACAAGGGCAA GTGGCATGGA GATGTTGCAG TAAAGATCCT AAAGGTGGTT 
GACCCAACTC CAGAGCAACT TCAGGCCTTC AGGAACGAGG TGGCTGTTTT GCGCAAAACA 
CGGCATGTTA ATATCCTGCT GTTCATGGGG TACATGACAA AGGACAACCT GGCGATTGTG 
ACCCAGTGGT GTGAAGGCAG CAGTCTCTAC AAACACCTGC ATGTCCAGGA GACCAAATTC 
CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACAGCTC AGGGAATGGA CTATTTACAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCCTC 
ACGGTGAAAA TCGGAGATTT TGGTTTGGCA ACAGTGAAGT CGCGCTGGAG TGGTTCTCAG 
CAGGTTGAAC AGCCCACTGG CTCTGTGCTG TGGATGGCCC CAGAAGTAAT CCGAATGCAG 
GATAACAACC CGTTCAGCTT CCAGTCCGAT GTCTACTCCT ATGGCATTGT GCTGTATGAG 
CTGATGACTG GGGAGCTTCC CTACTCCCAC ATCAACAACC GAGACCAGAT CATCTTCATG 
GTGGGCCGTG GGTACGCCTC CCCAGATCTT AGCAGGCTCT ACAAGAACTG CCCCAAGGCA 
ATGAAGAGGT TGGTGGCTGA CTGTGTGAAG AAAGTCAAAG AAGAAAGGCC TTTGTTTCCT 
CAGATCCTGT CTTCCATTGA GCTGCTTCAG CACTCTCTGC CGAAAATCAA CAGGAGCGCC 
TCTGAGCCTT CCCTGCATCG GGCAGCTCAC ACTGAGGACA TCAATGCTTG TACGCTGACC 
ACATCCCCAA GGCTACCAGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761440.1
CDS	187..2193
Translation	MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTASDSGVPAPPSFTMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIENNSLNASPRACSRRFCLRGRDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF

Target ORF information:

RefSeq Version	XM_008763218.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Raf-1 proto-oncogene, serine/threonine kinase (Raf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763218.2

ORF Insert Sequence:

ATGGAGCACA TACAGGGAGC TTGGAAGACG ATCAGCAATG GTTTCGGACT CAAAGATGCT 
GTGTTTGATG GCTCCAGTTG CATCTCCCCT ACAATTGTTC AGCAGTTTGG CTATCAGCGT 
CGGGCCTCTG ATGATGGCAA ACTCACGGAT TCTTCTAAGA CAAGCAATAC TATCCGGGTT 
TTCTTGCCGA ATAAGCAAAG GACTGTGGTC AATGTGCGGA ATGGGATGAG CTTGCACGAC 
TGCCTTATGA AAGCTCTGAA GGTTAGAGGC CTGCAGCCAG AGTGCTGTGC AGTGTTCAGA 
CTTCTTCAGG AGCACAAAGG TAAGAAAGCA CGCTTAGATT GGAACACCGA CGCCGCCTCT 
CTGATTGGAG AAGAACTGCA AGTGGATTTT TTGGATCACG TTCCACTCAC AACTCACAAC 
TTTGCTCGGA AAACGTTCCT GAAGCTTGCA TTCTGTGACA TCTGTCAAAA GTTCCTGCTA 
AATGGATTTC GATGTCAGAC TTGTGGCTAC AAGTTTCATG AGCACTGTAG CACCAAAGTA 
CCTACTATGT GTGTGGACTG GAGTAATATC AGACAGCTCT TGCTGTTTCC AAATTCCACT 
GCAAGTGACA GTGGAGTCCC AGCACCACCC TCTTTCACAA TGCGTCGGAT GCGAGAATCT 
GTTTCCCGGA TGCCTGCTAG TTCCCAGCAC AGATACTCCA CACCCCATGC CTTCACTTTC 
AACACCTCCA GCCCTTCCTC TGAAGGTTCC CTGTCCCAGA GGCAGAGGTC AACGTCCACT 
CCCAATGTCC ACATGGTCAG CACCACCCTG CCTGTGGACA GCAGGATGAT TGAGAATAAC 
AGCCTGAATG CTTCTCCCAG GGCGTGCTCC AGACGATTTT GTCTGAGGGG AAGAGATGCA 
ATTCGAAGTC ACAGTGAATC AGCCTCACCT TCAGCCCTGT CCAGCAGCCC CAACAACCTG 
AGCCCAACAG GCTGGTCACA GCCCAAAACC CCTGTGCCAG CACAAAGAGA GAGGGCGCCA 
GGATCTGGGA CCCAGGAAAA AAACAAAATT AGGCCTCGTG GGCAGAGAGA TTCAAGTTAT 
TACTGGGAAA TAGAAGCCAG TGAGGTGATG CTGTCTACTC GGATTGGCTC GGGCTCCTTT 
GGCACTGTGT ACAAGGGCAA GTGGCATGGA GATGTTGCAG TAAAGATCCT AAAGGTGGTT 
GACCCAACTC CAGAGCAACT TCAGGCCTTC AGGAACGAGG TGGCTGTTTT GCGCAAAACA 
CGGCATGTTA ATATCCTGCT GTTCATGGGG TACATGACAA AGGACAACCT GGCGATTGTG 
ACCCAGTGGT GTGAAGGCAG CAGTCTCTAC AAACACCTGC ATGTCCAGGA GACCAAATTC 
CAGATGTTCC AGCTAATTGA CATTGCCCGG CAGACAGCTC AGGGAATGGA CTATTTACAT 
GCAAAGAACA TCATCCACAG AGACATGAAA TCCAACAATA TATTTCTCCA TGAAGGCCTC 
ACGGTGAAAA TCGGAGATTT TGGTTTGGCA ACAGTGAAGT CGCGCTGGAG TGGTTCTCAG 
CAGGTTGAAC AGCCCACTGG CTCTGTGCTG TGGATGGCCC CAGAAGTAAT CCGAATGCAG 
GATAACAACC CGTTCAGCTT CCAGTCCGAT GTCTACTCCT ATGGCATTGT GCTGTATGAG 
CTGATGACTG GGGAGCTTCC CTACTCCCAC ATCAACAACC GAGACCAGAT CATCTTCATG 
GTGGGCCGTG GGTACGCCTC CCCAGATCTT AGCAGGCTCT ACAAGAACTG CCCCAAGGCA 
ATGAAGAGGT TGGTGGCTGA CTGTGTGAAG AAAGTCAAAG AAGAAAGGCC TTTGTTTCCT 
CAGATCCTGT CTTCCATTGA GCTGCTTCAG CACTCTCTGC CGAAAATCAA CAGGAGCGCC 
TCTGAGCCTT CCCTGCATCG GGCAGCTCAC ACTGAGGACA TCAATGCTTG TACGCTGACC 
ACATCCCCAA GGCTACCAGT CTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.
Biochemical and biophysical research communications483(1)82-87(2017 01)
Kamemura N,Murakami S,Komatsu H,Sawanoi M,Miyamoto K,Ishidoh K,Kishimoto K,Tsuji A,Yuasa K

Possible Role of Raf-1 Kinase in the Development of Cerebral Vasospasm and Early Brain Injury After Experimental Subarachnoid Hemorrhage in Rats.
Molecular neurobiology52(3)1527-1539(2015 Dec)
Zhang J,Xu X,Zhou D,Li H,You W,Wang Z,Chen G

Multikinase inhibitor sorafenib prevents pressure overload-induced left ventricular hypertrophy in rats by blocking the c-Raf/ERK1/2 signaling pathway.
Journal of cardiothoracic surgery981(2014 May)
Daryadel A,Bogdanova A,Gassmann M,Mueller X,Z?nd G,Seifert B,Lehalle C,Frossard N,Tavakoli R

Fibroblast growth factor-2 up-regulates the expression of nestin through the Ras-Raf-ERK-Sp1 signaling axis in C6 glioma cells.
Biochemical and biophysical research communications434(4)854-60(2013 May)
Chang KW,Huang YL,Wong ZR,Su PH,Huang BM,Ju TK,Yang HY

X-linked inhibitor of apoptosis protein negatively regulates neuronal differentiation through interaction with cRAF and Trk.
Scientific reports32397(2013)
Fad? R,Moubarak RS,Mi?ano-Molina AJ,Barneda-Zahonero B,Valero J,Saura CA,Moran J,Comella JX,Rodr?guez-?lvarez J

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Raf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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