Prkcg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkcg
Entrez Gene ID	24681
Full Name	protein kinase C, gamma
Synonyms	PKC,PKCI,Prkc,Prkcc,RATPKCI
General protein information	Preferred Names protein kinase C, gamma Names protein kinase C gamma type PKC-gamma protein kinase C type I (gamma type)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12
Summary	enriched in the neurons of the central nervous system and the spinal cord [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012628.1	NP_036760.1	protein kinase C gamma type
XM_006228014.3	XP_006228076.1	protein kinase C gamma type isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRKCG	XP_003316702.2
Bos taurus (cattle)	PRKCG	NP_001159974.1
Danio rerio (zebrafish)	prkcg	XP_001921715.1
Canis lupus familiaris (dog)	LOC100855828	XP_003638850.1
Homo sapiens (human)	PRKCG	NP_002730.1
Rattus norvegicus (Norway rat)	Prkcg	NP_036760.1
Canis lupus familiaris (dog)	LOC484316	XP_541432.4
Mus musculus (house mouse)	Prkcg	NP_035232.1
Xenopus tropicalis (tropical clawed frog)	prkcg	XP_004916388.1

Gene Ontology
Bibliography

Related articles in PubMed

PKC and Rab13 mediate Ca
Deng B, Zhu X, Zhao Y, Zhang D, Pannu A, Chen L, Niu W
Biochemical and biophysical research communications495(2)1956-1963(2018 Jan)

Functional communication between PKC-targeted cardiac troponin I phosphorylation sites.
Lang SE, Stevenson TK, Schatz TM, Biesiadecki BJ, Westfall MV
Archives of biochemistry and biophysics6271-9(2017 Aug)

Berberine Ameliorates Diabetic Neuropathy: TRPV1 Modulation by PKC Pathway.
Zan Y, Kuai CX, Qiu ZX, Huang F
The American journal of Chinese medicine45(8)1709-1723(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PKC phosphorylates cardiac troponin I (cTnI) S23/24, S43/45 and T144 to fine tune myocyte function.
Title: Functional communication between PKC-targeted cardiac troponin I phosphorylation sites.

In a model of cancer-induced bone pain, protein kinase C gamma is up-regulated in the spine.
Title: Involvement of Spinal CCR5/PKC? Signaling Pathway in the Maintenance of Cancer-Induced Bone Pain.

the intact subepidermal nerve fibers persistently increase PKCg activation and mTOR-dependent PKCg synthesis participate in the initiation and maintenance of mechanical hypersensitivity in spared nerve injury.
Title: Intact subepidermal nerve fibers mediate mechanical hypersensitivity via the activation of protein kinase C gamma in spared nerve injury.

Apelin-13 did increase ERK phosphorylation through the PI3K/Akt and PKC signaling pathways, resulting in changes in Egr-1 expression.
Title: Apelin-13 upregulates Egr-1 expression in rat vascular smooth muscle cells through the PI3K/Akt and PKC signaling pathways.

This study demonistrated that the PKCgamma interneuron within the rat medullary dorsal horn.
Title: Subpopulations of PKC? interneurons within the medullary dorsal horn revealed by electrophysiologic and morphologic approach.

The following Prkcg gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkcg cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa22226	XM_006228014.3 Latest version!	Rattus norvegicus protein kinase C, gamma (Prkcg), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa00495	NM_012628.1 Latest version!	Rattus norvegicus protein kinase C, gamma (Prkcg), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$412.30 $589.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkcg ( XM_006228014.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228076.1 Rattus norvegicus protein kinase C, gamma (Prkcg), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22226
Clone ID Related Accession (Same CDS sequence)	XM_006228014.3
Accession Version	XM_006228014.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C gamma type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228014.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGTC TGGGTCCTGG CGGGGGCGAC TCAGAAGGGG GACCCCGACC CCTGTTTTGC 
AGAAAGGGGG CGCTGAGGCA GAAGGTGGTC CACGAGGTGA AGAGCCACAA GTTCACCGCT 
CGTTTCTTCA AGCAGCCAAC CTTCTGCAGT CACTGTACCG ACTTCATCTG GGGCATTGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ACCGCCGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGAAAG GGCCCCCAGA CGGACGACCC TCGCAACAAG 
CACAAGTTCC GTCTGCACAG CTACAGCAGT CCCACCTTCT GCGACCACTG TGGTTCCCTC 
CTCTACGGGC TGGTGCACCA GGGCATGAAA TGTTCCTGTT GCGAAATGAA TGTGCACCGA 
CGCTGTGTGC GCAGCGTGCC CTCCCTTTGC GGCGTGGACC ATACAGAGCG CCGTGGACGT 
CTGCAACTGG AAATCCGGGC TCCCACATCA GATGAGATCC ATATTACTGT GGGTGAGGCC 
CGGAACCTCA TTCCTATGGA CCCCAATGGC CTGTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCGGACC CTCGGAACCT GACAAAACAG AAGACAAAGA CCGTGAAAGC CACACTGAAT 
CCCGTGTGGA ACGAGACCTT CGTGTTCAAC CTGAAGCCGG GGGATGTGGA GCGCCGGCTC 
AGTGTGGAGG TGTGGGATTG GGATAGGACA TCCCGAAATG ACTTCATGGG TGCCATGTCC 
TTTGGTGTCT CAGAGCTACT CAAGGCTCCT GTGGATGGAT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTACCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CCTGTAATTA CCCCTTGGAA TTGTATGAGG TGATGCTGGC AGAGCGCAGA 
GGATCCGATG AACTCTATGC CATCAAGATA CTGAAAAAAG ACGTCATTGT CCAGGATGAT 
GATGTAGACT GCACCCTTGT GGAGAAGCGT GTGCTGGCAT TGGGAGGCCG AGGTCCTGGA 
GGCCGGCCAC ACTTTCTCAC ACAACTTCAT TCCACCTTTC AGACTCCGGA CCGCCTGTAT 
TTTGTGATGG AGTACGTCAC TGGGGGCGAT TTAATGTACC ACATTCAGCA ACTGGGCAAG 
TTTAAGGAGC CCCACGCAGC ATTCTATGCC GCGGAAATCG CCATAGGCCT CTTCTTCCTT 
CACAACCAGG GCATCATCTA CAGGGACCTC AAGTTGGATA ATGTGATGCT GGATGCTGAA 
GGACACATCA AGATCACAGA CTTCGGCATG TGTAAAGAGA ATGTCTTCCC TGGGTCCACA 
ACCCGCACCT TCTGTGGGAC CCCAGACTAC ATAGCACCTG AGATCATTGC CTATCAGCCC 
TATGGGAAGT CTGTCGACTG GTGGTCCTTT GGAGTCCTGC TGTATGAGAT GTTGGCAGGA 
CAGCCACCCT TTGATGGGGA AGATGAGGAG GAGCTGTTTC AAGCCATCAT GGAACAAACT 
GTCACCTATC CCAAGTCACT TTCCCGGGAA GCTGTGGCCA TCTGCAAGGG GTTCCTGACC 
AAGCACCCAG GAAAGCGCCT GGGCTCAGGG CCAGATGGGG AACCCACCAT CCGGGCTCAT 
GGCTTTTTCC GTTGGATCGA TTGGGAGAGG TTGGAGAGAC TGGAAATTGC GCCTCCTTTT 
AGACCACGTC CGTGTGGCCG CAGCGGCGAA AACTTTGACA AGTTCTTCAC GCGGGCAGCG 
CCAGCCTTGA CCCCGCCAGA CCGCTTGGTC CTAGCCAGCA TCGACCAAGC TGATTTCCAG 
GGCTTTACTT ATGTGAACCC GGACTTCGTG CACCCAGATG CCCGCAGCCC CACAAGCCCT 
GTGCCTGTGC CCGTCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228076.1
CDS	282..2222
Translation	MAGLGPGGGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTSDEIHITVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTKTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYEVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGSTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIAPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

Target ORF information:

RefSeq Version	XM_006228014.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, gamma (Prkcg), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228014.3

ORF Insert Sequence:

ATGGCGGGTC TGGGTCCTGG CGGGGGCGAC TCAGAAGGGG GACCCCGACC CCTGTTTTGC 
AGAAAGGGGG CGCTGAGGCA GAAGGTGGTC CACGAGGTGA AGAGCCACAA GTTCACCGCT 
CGTTTCTTCA AGCAGCCAAC CTTCTGCAGT CACTGTACCG ACTTCATCTG GGGCATTGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ACCGCCGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG CGCTGGAAAG GGCCCCCAGA CGGACGACCC TCGCAACAAG 
CACAAGTTCC GTCTGCACAG CTACAGCAGT CCCACCTTCT GCGACCACTG TGGTTCCCTC 
CTCTACGGGC TGGTGCACCA GGGCATGAAA TGTTCCTGTT GCGAAATGAA TGTGCACCGA 
CGCTGTGTGC GCAGCGTGCC CTCCCTTTGC GGCGTGGACC ATACAGAGCG CCGTGGACGT 
CTGCAACTGG AAATCCGGGC TCCCACATCA GATGAGATCC ATATTACTGT GGGTGAGGCC 
CGGAACCTCA TTCCTATGGA CCCCAATGGC CTGTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCGGACC CTCGGAACCT GACAAAACAG AAGACAAAGA CCGTGAAAGC CACACTGAAT 
CCCGTGTGGA ACGAGACCTT CGTGTTCAAC CTGAAGCCGG GGGATGTGGA GCGCCGGCTC 
AGTGTGGAGG TGTGGGATTG GGATAGGACA TCCCGAAATG ACTTCATGGG TGCCATGTCC 
TTTGGTGTCT CAGAGCTACT CAAGGCTCCT GTGGATGGAT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTACCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CCTGTAATTA CCCCTTGGAA TTGTATGAGG TGATGCTGGC AGAGCGCAGA 
GGATCCGATG AACTCTATGC CATCAAGATA CTGAAAAAAG ACGTCATTGT CCAGGATGAT 
GATGTAGACT GCACCCTTGT GGAGAAGCGT GTGCTGGCAT TGGGAGGCCG AGGTCCTGGA 
GGCCGGCCAC ACTTTCTCAC ACAACTTCAT TCCACCTTTC AGACTCCGGA CCGCCTGTAT 
TTTGTGATGG AGTACGTCAC TGGGGGCGAT TTAATGTACC ACATTCAGCA ACTGGGCAAG 
TTTAAGGAGC CCCACGCAGC ATTCTATGCC GCGGAAATCG CCATAGGCCT CTTCTTCCTT 
CACAACCAGG GCATCATCTA CAGGGACCTC AAGTTGGATA ATGTGATGCT GGATGCTGAA 
GGACACATCA AGATCACAGA CTTCGGCATG TGTAAAGAGA ATGTCTTCCC TGGGTCCACA 
ACCCGCACCT TCTGTGGGAC CCCAGACTAC ATAGCACCTG AGATCATTGC CTATCAGCCC 
TATGGGAAGT CTGTCGACTG GTGGTCCTTT GGAGTCCTGC TGTATGAGAT GTTGGCAGGA 
CAGCCACCCT TTGATGGGGA AGATGAGGAG GAGCTGTTTC AAGCCATCAT GGAACAAACT 
GTCACCTATC CCAAGTCACT TTCCCGGGAA GCTGTGGCCA TCTGCAAGGG GTTCCTGACC 
AAGCACCCAG GAAAGCGCCT GGGCTCAGGG CCAGATGGGG AACCCACCAT CCGGGCTCAT 
GGCTTTTTCC GTTGGATCGA TTGGGAGAGG TTGGAGAGAC TGGAAATTGC GCCTCCTTTT 
AGACCACGTC CGTGTGGCCG CAGCGGCGAA AACTTTGACA AGTTCTTCAC GCGGGCAGCG 
CCAGCCTTGA CCCCGCCAGA CCGCTTGGTC CTAGCCAGCA TCGACCAAGC TGATTTCCAG 
GGCTTTACTT ATGTGAACCC GGACTTCGTG CACCCAGATG CCCGCAGCCC CACAAGCCCT 
GTGCCTGTGC CCGTCATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prkcg ( NM_012628.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036760.1 Rattus norvegicus protein kinase C, gamma (Prkcg), mRNA.

Price & Availability↑

CloneID	ORa00495
Clone ID Related Accession (Same CDS sequence)	NM_012628.1
Accession Version	NM_012628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Rattus norvegicus(Norway rat)
Product	protein kinase C gamma type
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M13707.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M13707.1, BC089226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGTC TGGGTCCTGG CGGGGGCGAC TCAGAAGGGG GACCCCGACC CCTGTTTTGC 
AGAAAGGGGG CGCTGAGGCA GAAGGTGGTC CACGAGGTGA AGAGCCACAA GTTCACCGCT 
CGTTTCTTCA AGCAGCCAAC CTTCTGCAGT CACTGTACCG ACTTCATCTG GGGCATTGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ACCGCCGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG AGCTGGAAAG GGCCCCCAGA CGGACGACCC TCGCAACAAG 
CACAAGTTCC GTCTGCACAG CTACAGCAGT CCCACCTTCT GCGACCACTG TGGTTCCCTC 
CTCTACGGGC TGGTGCACCA GGGCATGAAA TGTTCCTGTT GCGAAATGAA TGTGCACCGA 
CGCTGTGTGC GCAGCGTGCC CTCCCTTTGC GGCGTGGACC ATACAGAGCG CCGTGGACGT 
CTGCAACTGG AAATCCGGGC TCCCACATCA GATGAGATCC ATATTACTGT GGGTGAGGCC 
CGGAACCTCA TTCCTATGGA CCCCAATGGC CTGTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCGGACC CTCGGAACCT GACAAAACAG AAGACAAAGA CCGTGAAAGC CACACTGAAT 
CCCGTGTGGA ACGAGACCTT CGTGTTCAAC CTGAAGCCGG GGGATGTGGA GCGCCGGCTC 
AGTGTGGAGG TGTGGGATTG GGATAGGACA TCCCGAAATG ACTTCATGGG TGCCATGTCC 
TTTGGTGTCT CAGAGCTACT CAAGGCTCCT GTGGATGGAT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTACCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CCTGTAATTA CCCCTTGGAA TTGTATGAGA GAGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TTCCTTCTCC ATCCCCCAGT CCCACGGACT CCAAGAGATG CTTCTTCGGT 
GCCAGCCCAG GACGCCTGCA TATCTCTGAC TTCAGCTTCC TCATGGTTCT AGGGAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCAGAGCGC AGAGGATCCG ATGAACTCTA TGCCATCAAG 
ATACTGAAAA AAGACGTCAT TGTCCAGGAT GATGATGTAG ACTGCACCCT TGTGGAGAAG 
CGTGTGCTGG CATTGGGAGG CCGAGGTCCT GGAGGCCGGC CACACTTTCT CACACAACTT 
CATTCCACCT TTCAGACTCC GGACCGCCTG TATTTTGTGA TGGAGTACGT CACTGGGGGC 
GATTTAATGT ACCACATTCA GCAACTGGGC AAGTTTAAGG AGCCCCACGC AGCATTCTAT 
GCCGCGGAAA TCGCCATAGG CCTCTTCTTC CTTCACAACC AGGGCATCAT CTACAGGGAC 
CTCAAGTTGG ATAATGTGAT GCTGGATGCT GAAGGACACA TCAAGATCAC AGACTTCGGC 
ATGTGTAAAG AGAATGTCTT CCCTGGGTCC ACAACCCGCA CCTTCTGTGG GACCCCAGAC 
TACATAGCAC CTGAGATCAT TGCCTATCAG CCCTATGGGA AGTCTGTCGA CTGGTGGTCC 
TTTGGAGTCC TGCTGTATGA GATGTTGGCA GGACAGCCAC CCTTTGATGG GGAAGATGAG 
GAGGAGCTGT TTCAAGCCAT CATGGAACAA ACTGTCACCT ATCCCAAGTC ACTTTCCCGG 
GAAGCTGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGAAAGCG CCTGGGCTCA 
GGGCCAGATG GGGAACCCAC CATCCGGGCT CATGGCTTTT TCCGTTGGAT CGATTGGGAG 
AGGTTGGAGA GACTGGAAAT TGCGCCTCCT TTTAGACCAC GTCCGTGTGG CCGCAGCGGC 
GAAAACTTTG ACAAGTTCTT CACGCGGGCA GCGCCAGCCT TGACCCCGCC AGACCGCTTG 
GTCCTAGCCA GCATCGACCA AGCTGATTTC CAGGGCTTTA CTTATGTGAA CCCGGACTTC 
GTGCACCCAG ATGCCCGCAG CCCCACAAGC CCTGTGCCTG TGCCCGTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036760.1
CDS	219..2312
Translation	MAGLGPGGGDSEGGPRPLFCRKGALRQKVVHEVKSHKFTARFFKQPTFCSHCTDFIWGIGKQGLQCQVCSFVVHRRCHEFVTFECPGAGKGPQTDDPRNKHKFRLHSYSSPTFCDHCGSLLYGLVHQGMKCSCCEMNVHRRCVRSVPSLCGVDHTERRGRLQLEIRAPTSDEIHITVGEARNLIPMDPNGLSDPYVKLKLIPDPRNLTKQKTKTVKATLNPVWNETFVFNLKPGDVERRLSVEVWDWDRTSRNDFMGAMSFGVSELLKAPVDGWYKLLNQEEGEYYNVPVADADNCSLLQKFEACNYPLELYERVRMGPSSSPIPSPSPSPTDSKRCFFGASPGRLHISDFSFLMVLGKGSFGKVMLAERRGSDELYAIKILKKDVIVQDDDVDCTLVEKRVLALGGRGPGGRPHFLTQLHSTFQTPDRLYFVMEYVTGGDLMYHIQQLGKFKEPHAAFYAAEIAIGLFFLHNQGIIYRDLKLDNVMLDAEGHIKITDFGMCKENVFPGSTTRTFCGTPDYIAPEIIAYQPYGKSVDWWSFGVLLYEMLAGQPPFDGEDEEELFQAIMEQTVTYPKSLSREAVAICKGFLTKHPGKRLGSGPDGEPTIRAHGFFRWIDWERLERLEIAPPFRPRPCGRSGENFDKFFTRAAPALTPPDRLVLASIDQADFQGFTYVNPDFVHPDARSPTSPVPVPVM

Target ORF information:

RefSeq Version	NM_012628.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase C, gamma (Prkcg), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012628.1

ORF Insert Sequence:

ATGGCGGGTC TGGGTCCTGG CGGGGGCGAC TCAGAAGGGG GACCCCGACC CCTGTTTTGC 
AGAAAGGGGG CGCTGAGGCA GAAGGTGGTC CACGAGGTGA AGAGCCACAA GTTCACCGCT 
CGTTTCTTCA AGCAGCCAAC CTTCTGCAGT CACTGTACCG ACTTCATCTG GGGCATTGGA 
AAGCAGGGCC TGCAATGTCA AGTCTGCAGC TTTGTGGTTC ACCGCCGATG CCACGAATTT 
GTGACCTTCG AGTGTCCAGG AGCTGGAAAG GGCCCCCAGA CGGACGACCC TCGCAACAAG 
CACAAGTTCC GTCTGCACAG CTACAGCAGT CCCACCTTCT GCGACCACTG TGGTTCCCTC 
CTCTACGGGC TGGTGCACCA GGGCATGAAA TGTTCCTGTT GCGAAATGAA TGTGCACCGA 
CGCTGTGTGC GCAGCGTGCC CTCCCTTTGC GGCGTGGACC ATACAGAGCG CCGTGGACGT 
CTGCAACTGG AAATCCGGGC TCCCACATCA GATGAGATCC ATATTACTGT GGGTGAGGCC 
CGGAACCTCA TTCCTATGGA CCCCAATGGC CTGTCTGATC CCTATGTGAA ACTGAAGCTC 
ATCCCGGACC CTCGGAACCT GACAAAACAG AAGACAAAGA CCGTGAAAGC CACACTGAAT 
CCCGTGTGGA ACGAGACCTT CGTGTTCAAC CTGAAGCCGG GGGATGTGGA GCGCCGGCTC 
AGTGTGGAGG TGTGGGATTG GGATAGGACA TCCCGAAATG ACTTCATGGG TGCCATGTCC 
TTTGGTGTCT CAGAGCTACT CAAGGCTCCT GTGGATGGAT GGTACAAGTT ACTGAACCAG 
GAGGAGGGCG AGTATTACAA TGTACCGGTG GCCGATGCTG ACAACTGCAG CCTCCTCCAG 
AAGTTTGAGG CCTGTAATTA CCCCTTGGAA TTGTATGAGA GAGTGCGGAT GGGCCCCTCT 
TCCTCTCCCA TTCCTTCTCC ATCCCCCAGT CCCACGGACT CCAAGAGATG CTTCTTCGGT 
GCCAGCCCAG GACGCCTGCA TATCTCTGAC TTCAGCTTCC TCATGGTTCT AGGGAAAGGC 
AGTTTTGGGA AGGTGATGCT GGCAGAGCGC AGAGGATCCG ATGAACTCTA TGCCATCAAG 
ATACTGAAAA AAGACGTCAT TGTCCAGGAT GATGATGTAG ACTGCACCCT TGTGGAGAAG 
CGTGTGCTGG CATTGGGAGG CCGAGGTCCT GGAGGCCGGC CACACTTTCT CACACAACTT 
CATTCCACCT TTCAGACTCC GGACCGCCTG TATTTTGTGA TGGAGTACGT CACTGGGGGC 
GATTTAATGT ACCACATTCA GCAACTGGGC AAGTTTAAGG AGCCCCACGC AGCATTCTAT 
GCCGCGGAAA TCGCCATAGG CCTCTTCTTC CTTCACAACC AGGGCATCAT CTACAGGGAC 
CTCAAGTTGG ATAATGTGAT GCTGGATGCT GAAGGACACA TCAAGATCAC AGACTTCGGC 
ATGTGTAAAG AGAATGTCTT CCCTGGGTCC ACAACCCGCA CCTTCTGTGG GACCCCAGAC 
TACATAGCAC CTGAGATCAT TGCCTATCAG CCCTATGGGA AGTCTGTCGA CTGGTGGTCC 
TTTGGAGTCC TGCTGTATGA GATGTTGGCA GGACAGCCAC CCTTTGATGG GGAAGATGAG 
GAGGAGCTGT TTCAAGCCAT CATGGAACAA ACTGTCACCT ATCCCAAGTC ACTTTCCCGG 
GAAGCTGTGG CCATCTGCAA GGGGTTCCTG ACCAAGCACC CAGGAAAGCG CCTGGGCTCA 
GGGCCAGATG GGGAACCCAC CATCCGGGCT CATGGCTTTT TCCGTTGGAT CGATTGGGAG 
AGGTTGGAGA GACTGGAAAT TGCGCCTCCT TTTAGACCAC GTCCGTGTGG CCGCAGCGGC 
GAAAACTTTG ACAAGTTCTT CACGCGGGCA GCGCCAGCCT TGACCCCGCC AGACCGCTTG 
GTCCTAGCCA GCATCGACCA AGCTGATTTC CAGGGCTTTA CTTATGTGAA CCCGGACTTC 
GTGCACCCAG ATGCCCGCAG CCCCACAAGC CCTGTGCCTG TGCCCGTCAT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PKC and Rab13 mediate Ca
Biochemical and biophysical research communications495(2)1956-1963(2018 Jan)
Deng B,Zhu X,Zhao Y,Zhang D,Pannu A,Chen L,Niu W

Functional communication between PKC-targeted cardiac troponin I phosphorylation sites.
Archives of biochemistry and biophysics6271-9(2017 Aug)
Lang SE,Stevenson TK,Schatz TM,Biesiadecki BJ,Westfall MV

Berberine Ameliorates Diabetic Neuropathy: TRPV1 Modulation by PKC Pathway.
The American journal of Chinese medicine45(8)1709-1723(2017)
Zan Y,Kuai CX,Qiu ZX,Huang F

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Prkcg cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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