Ppm1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppm1b
Entrez Gene ID	24667
Full Name	protein phosphatase, Mg2+/Mn2+ dependent, 1B
Synonyms	Pp2c2
General protein information	Preferred Names protein phosphatase, Mg2+/Mn2+ dependent, 1B Names protein phosphatase 1B PP2C-beta Protein phosphatase type 1B (formely 2C) Mg-dependent beta isoform Protein phosphatase type 1B (formely 2C), Mg-dependent, beta isoform protein phosphatase 1B, magnesium dependent, beta isoform protein phosphatase 2C isoform beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_033096.2	NP_149087.1	protein phosphatase 1B isoform 1
XM_008764454.2	XP_008762676.1	protein phosphatase 1B isoform X2
NM_001270619.1	NP_001257548.1	protein phosphatase 1B isoform 2
XM_017594036.1	XP_017449525.1	protein phosphatase 1B isoform X1
NM_001270620.1	NP_001257549.1	protein phosphatase 1B isoform 3
XM_017594035.1	XP_017449524.1	protein phosphatase 1B isoform X1

Pathways from BioSystems

Homologies

	PPM1B	XP_001111662.1
Bos taurus (cattle)	PPM1B	NP_776855.1
Mus musculus (house mouse)	Ppm1b	NP_001152968.1
Pan troglodytes (chimpanzee)	PPM1B	XP_525747.1
Rattus norvegicus (Norway rat)	Ppm1b	NP_001257548.1
Danio rerio (zebrafish)	ppm1ba	NP_571473.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008149	XP_317314.3
Xenopus tropicalis (tropical clawed frog)	ppm1b	NP_001008030.1
Homo sapiens (human)	PPM1B	NP_002697.1
Canis lupus familiaris (dog)	PPM1B	XP_005626126.1
Caenorhabditis elegans (roundworm)	ppm-1	NP_001122929.1
Gallus gallus (chicken)	PPM1B	NP_001026223.1
Drosophila melanogaster (fruit fly)	alph	NP_651701.1

Gene Ontology
Bibliography

Related articles in PubMed

N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells.
Chida T, Ando M, Matsuki T, Masu Y, Nagaura Y, Takano-Yamamoto T, Tamura S, Kobayashi T
The Biochemical journal449(3)741-9(2013 Feb)

PPM1B negatively regulates antiviral response via dephosphorylating TBK1.
Zhao Y, Liang L, Fan Y, Sun S, An L, Shi Z, Cheng J, Jia W, Sun W, Mori-Akiyama Y, Zhang H, Fu S, Yang J
Cellular signalling24(11)2197-204(2012 Nov)

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Voss M, Paterson J, Kelsall IR, Mart?n-Granados C, Hastie CJ, Peggie MW, Cohen PT
Cellular signalling23(1)114-24(2011 Jan)

PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation.
Sun W, Yu Y, Dotti G, Shen T, Tan X, Savoldo B, Pass AK, Chu M, Zhang D, Lu X, Fu S, Lin X, Yang J
Cellular signalling21(1)95-102(2009 Jan)

Differentiation-dependent enhanced expression of protein phosphatase 2Cbeta in germ cells of mouse seminiferous tubules.
Kato S, Kobayashi T, Kusuda K, Nishina Y, Nishimune Y, Yomogida K, Yamamoto M, Sakagami H, Kondo H, Ohnishi M, Chida N, Yanagawa Y, Tamura S
FEBS letters396(2-3)293-7(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppm1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppm1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09408	NM_001270620.1 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09408	XM_017594036.1 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09408	XM_017594035.1 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09065	NM_033096.2 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa09406	NM_001270619.1 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42943	XM_008764454.2 Latest version!	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppm1b ( NM_001270620.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257549.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa09408
Clone ID Related Accession (Same CDS sequence)	XM_017594036.1 , NM_001270620.1 , XM_017594035.1
Accession Version	NM_001270620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1B isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC081762.1. Transcript Variant: This variant (3) differs at the 5' and 3' ends compared to variant 1, which results in a slightly longer isoform (3) with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC081762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642715, SAMN02642719 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGTTTTAC 
CAACCTTCAA TTCCATACAG TGACAATGTT TTCCTATTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257549.1
CDS	879..2060
Translation	MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAADKSGFALEPSVENVKTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPTHIYFINCGDSRAVLCRNGQVCFSTQDHKPCNPMEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFVVLACDGIWDVMSNEELCEFVNSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFANAPKVSDEAVKRDLELDKHLESRVEEIMQKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPNKDNDGFYQPSIPYSDNVFLL

Target ORF information:

RefSeq Version	NM_001270620.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270620.1

ORF Insert Sequence:

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGTTTTAC 
CAACCTTCAA TTCCATACAG TGACAATGTT TTCCTATTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1b ( XM_017594036.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449525.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa09408
Clone ID Related Accession (Same CDS sequence)	XM_017594036.1 , NM_001270620.1 , XM_017594035.1
Accession Version	XM_017594036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGTTTTAC 
CAACCTTCAA TTCCATACAG TGACAATGTT TTCCTATTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449525.1
CDS	400..1581
Translation	MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAADKSGFALEPSVENVKTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPTHIYFINCGDSRAVLCRNGQVCFSTQDHKPCNPMEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFVVLACDGIWDVMSNEELCEFVNSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFANAPKVSDEAVKRDLELDKHLESRVEEIMQKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPNKDNDGFYQPSIPYSDNVFLL

Target ORF information:

RefSeq Version	XM_017594036.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594036.1

ORF Insert Sequence:

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGTTTTAC 
CAACCTTCAA TTCCATACAG TGACAATGTT TTCCTATTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1b ( XM_017594035.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017449524.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa09408
Clone ID Related Accession (Same CDS sequence)	XM_017594036.1 , NM_001270620.1 , XM_017594035.1
Accession Version	XM_017594035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGTTTTAC 
CAACCTTCAA TTCCATACAG TGACAATGTT TTCCTATTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017449524.1
CDS	400..1581
Translation	MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAADKSGFALEPSVENVKTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPTHIYFINCGDSRAVLCRNGQVCFSTQDHKPCNPMEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFVVLACDGIWDVMSNEELCEFVNSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFANAPKVSDEAVKRDLELDKHLESRVEEIMQKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPNKDNDGFYQPSIPYSDNVFLL

Target ORF information:

RefSeq Version	XM_017594035.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594035.1

ORF Insert Sequence:

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGTTTTAC 
CAACCTTCAA TTCCATACAG TGACAATGTT TTCCTATTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1b ( NM_033096.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_149087.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 1, mRNA.

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CloneID	ORa09065
Clone ID Related Accession (Same CDS sequence)	NM_033096.2
Accession Version	NM_033096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1B isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FN800175.1, AC111831.5, FM108050.1, FM075941.1, CV110469.1, FM104054.1 and FM141620.1. On Jul 21, 2012 this sequence version replaced NM_033096.1. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AJ271837.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGGGCGCT 
GGCGATCTAG AAGACTCATT GGTAGCCTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149087.1
CDS	405..1577
Translation	MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAADKSGFALEPSVENVKTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPTHIYFINCGDSRAVLCRNGQVCFSTQDHKPCNPMEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFVVLACDGIWDVMSNEELCEFVNSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFANAPKVSDEAVKRDLELDKHLESRVEEIMQKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPNKDNDGGAGDLEDSLVAL

Target ORF information:

RefSeq Version	NM_033096.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033096.2

ORF Insert Sequence:

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGGGCGCT 
GGCGATCTAG AAGACTCATT GGTAGCCTTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1b ( NM_001270619.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257548.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 2, mRNA.

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CloneID	ORa09406
Clone ID Related Accession (Same CDS sequence)	NM_001270619.1
Accession Version	NM_001270619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1398bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1B isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FN800175.1, AC111831.5, FM108050.1, FM075941.1, FM042005.1 and BQ780970.1. Transcript Variant: This variant (2) contains an alternate 3' terminal exon compared to variant 1, which results in a longer isoform (2) with a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC061986.1, AJ271834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGGCCTCC 
GAGGAAGCAG AGGAAAGTGG ATCACAGGGG AAATTGGTGG AAGCTCTCAG GCAAATGAGA 
GTTAACCATA GGGGCAACTA CCGACAACTG CTGGAGGAGA TGCTGACTAG TTACAGGCTA 
GCTAAAGTAG AGGGAGAAGA GAGCCCGGCT GGCCCAGCTG CTGCAACTGC TTCTTCGAAC 
AGTGATGCTG GAAACCCAGT GGCAGTGCAG GAGAGACACA CTGAAAGTGG TCCTGCTGAA 
TTAGACAGCC CTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257548.1
CDS	405..1802
Translation	MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAADKSGFALEPSVENVKTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPTHIYFINCGDSRAVLCRNGQVCFSTQDHKPCNPMEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFVVLACDGIWDVMSNEELCEFVNSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFANAPKVSDEAVKRDLELDKHLESRVEEIMQKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPNKDNDGASEEAEESGSQGKLVEALRQMRVNHRGNYRQLLEEMLTSYRLAKVEGEESPAGPAAATASSNSDAGNPVAVQERHTESGPAELDSPK

Target ORF information:

RefSeq Version	NM_001270619.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270619.1

ORF Insert Sequence:

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGGCCTCC 
GAGGAAGCAG AGGAAAGTGG ATCACAGGGG AAATTGGTGG AAGCTCTCAG GCAAATGAGA 
GTTAACCATA GGGGCAACTA CCGACAACTG CTGGAGGAGA TGCTGACTAG TTACAGGCTA 
GCTAAAGTAG AGGGAGAAGA GAGCCCGGCT GGCCCAGCTG CTGCAACTGC TTCTTCGAAC 
AGTGATGCTG GAAACCCAGT GGCAGTGCAG GAGAGACACA CTGAAAGTGG TCCTGCTGAA 
TTAGACAGCC CTAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppm1b ( XM_008764454.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762676.1 Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa42943
Clone ID Related Accession (Same CDS sequence)	XM_008764454.2
Accession Version	XM_008764454.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGATGGCA 
GACTTGAGCA CGTCTATTTG TAAAGCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762676.1
CDS	400..1572
Translation	MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVEMEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANYCSTHLLEHITTNEDFRAADKSGFALEPSVENVKTGIRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMISPTHIYFINCGDSRAVLCRNGQVCFSTQDHKPCNPMEKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCVDGKGPTEQLVSPEPEVYEILRAEEDEFVVLACDGIWDVMSNEELCEFVNSRLEVSDDLENVCNWVVDTCLHKGSRDNMSIVLVCFANAPKVSDEAVKRDLELDKHLESRVEEIMQKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPNKDNDGMADLSTSICKAS

Target ORF information:

RefSeq Version	XM_008764454.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase, Mg2+/Mn2+ dependent, 1B (Ppm1b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764454.2

ORF Insert Sequence:

ATGGGTGCAT TTTTGGATAA ACCCAAAACT GAAAAGCACA ATGCTCACGG TGCAGGGAAC 
GGTCTGCGTT ACGGTCTGAG CAGTATGCAA GGATGGAGAG TAGAAATGGA AGACGCACAC 
ACTGCTGTTG TGGGAATTCC TCACGGCTTG GAGGACTGGT CGTTTTTTGC AGTCTATGAT 
GGTCATGCTG GATCCCGAGT GGCAAATTAC TGTTCAACAC ATCTATTAGA ACACATCACT 
ACCAATGAAG ACTTTAGGGC AGCTGACAAA TCAGGCTTTG CTCTTGAGCC GTCAGTGGAA 
AATGTTAAGA CTGGTATCCG AACTGGCTTT TTGAAAATTG ATGAATATAT GCGTAACTTT 
TCAGACCTGA GGAACGGGAT GGACAGGAGC GGCTCTACCG CAGTGGGCGT GATGATTTCA 
CCGACACACA TCTACTTTAT CAACTGCGGT GACTCGAGAG CTGTTCTGTG TAGGAATGGA 
CAAGTCTGCT TTTCTACCCA GGATCACAAA CCTTGTAATC CAATGGAAAA GGAGCGCATC 
CAAAATGCAG GAGGCAGTGT AATGATACAG CGTGTGAATG GGTCGTTAGC AGTGTCTCGT 
GCTCTGGGGG ACTATGATTA CAAGTGTGTT GATGGCAAGG GCCCCACAGA GCAGCTTGTT 
TCTCCAGAGC CTGAGGTTTA TGAGATTCTA AGAGCAGAAG AGGATGAATT TGTCGTCCTG 
GCTTGTGATG GGATCTGGGA TGTGATGAGC AATGAGGAGC TCTGTGAGTT TGTTAACTCT 
AGGCTTGAGG TGTCAGACGA CCTGGAGAAT GTGTGCAATT GGGTAGTGGA CACTTGTTTA 
CATAAGGGAA GTCGAGATAA CATGAGTATT GTGTTAGTTT GCTTTGCAAA TGCCCCCAAG 
GTCTCAGATG AAGCCGTGAA AAGAGATTTA GAGTTGGACA AGCACTTGGA ATCACGGGTG 
GAAGAAATCA TGCAGAAGTC TGGAGAGGAA GGAATGCCTG ATCTTGCCCA TGTGATGCGC 
ATTTTGTCTG CAGAAAATAT CCCAAATTTA CCTCCCGGGG GAGGCCTCGC TGGCAAGCGC 
AATGTTATTG AAGCTGTTTA TAGTAGACTT AATCCAAACA AAGACAATGA TGGGATGGCA 
GACTTGAGCA CGTCTATTTG TAAAGCCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

N-Myristoylation is essential for protein phosphatases PPM1A and PPM1B to dephosphorylate their physiological substrates in cells.
The Biochemical journal449(3)741-9(2013 Feb)
Chida T,Ando M,Matsuki T,Masu Y,Nagaura Y,Takano-Yamamoto T,Tamura S,Kobayashi T

PPM1B negatively regulates antiviral response via dephosphorylating TBK1.
Cellular signalling24(11)2197-204(2012 Nov)
Zhao Y,Liang L,Fan Y,Sun S,An L,Shi Z,Cheng J,Jia W,Sun W,Mori-Akiyama Y,Zhang H,Fu S,Yang J

Ppm1E is an in cellulo AMP-activated protein kinase phosphatase.
Cellular signalling23(1)114-24(2011 Jan)
Voss M,Paterson J,Kelsall IR,Mart?n-Granados C,Hastie CJ,Peggie MW,Cohen PT

PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha-induced IKKbeta-NF-kappaB activation.
Cellular signalling21(1)95-102(2009 Jan)
Sun W,Yu Y,Dotti G,Shen T,Tan X,Savoldo B,Pass AK,Chu M,Zhang D,Lu X,Fu S,Lin X,Yang J

Differentiation-dependent enhanced expression of protein phosphatase 2Cbeta in germ cells of mouse seminiferous tubules.
FEBS letters396(2-3)293-7(1996 Nov)
Kato S,Kobayashi T,Kusuda K,Nishina Y,Nishimune Y,Yomogida K,Yamamoto M,Sakagami H,Kondo H,Ohnishi M,Chida N,Yanagawa Y,Tamura S

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Ppm1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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