Pmch cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pmch
Entrez Gene ID	24659
Full Name	pro-melanin-concentrating hormone
General protein information	Preferred Names pro-melanin-concentrating hormone Names pro-MCH
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	cyclic neuropeptide; induces hippocampal synaptic transmission [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012625.1	NP_036757.1	pro-MCH precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PMCH	NP_001005755.1
Mus musculus (house mouse)	Pmch	NP_084247.1
Homo sapiens (human)	PMCH	NP_002665.2
Pan troglodytes (chimpanzee)	PMCH	NP_001128240.1
Bos taurus (cattle)	PMCH	NP_001073709.1
Rattus norvegicus (Norway rat)	Pmch	NP_036757.1
Gallus gallus (chicken)	PMCH	NP_001182724.1
Xenopus tropicalis (tropical clawed frog)	pmch	XP_002936876.1

Gene Ontology
Bibliography

Related articles in PubMed

Litter size determines the number of melanin-concentrating hormone neurons in the medial preoptic area of Sprague Dawley lactating dams.
Ferreira JGP, Duarte JCG, Diniz GB, Bittencourt JC
Physiology & behavior18175-79(2017 Nov)

Neuropeptide glutamic acid-isoleucine (NEI)-induced paradoxical sleep in rats.
Fujimoto M, Fukuda S, Sakamoto H, Takata J, Sawamura S
Peptides8728-33(2017 Jan)

MCH levels in the CSF, brain preproMCH and MCHR1 gene expression during paradoxical sleep deprivation, sleep rebound and chronic sleep restriction.
Dias Abdo Agamme AL, Aguilar Calegare BF, Fernandes L, Costa A, Lagos P, Torterolo P, D'Almeida V
Peptides749-15(2015 Dec)

Melanin-concentrating hormone in the medial preoptic area reduces active components of maternal behavior in rats.
Benedetto L, Pereira M, Ferreira A, Torterolo P
Peptides5820-5(2014 Aug)

The role of melanin concentrating hormone (MCH) in the central chemoreflex: a knockdown study by siRNA in the lateral hypothalamus in rats.
Li N, Nattie E, Li A
PloS one9(8)e103585(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

our findings demonstrate that NEI, a cleaved peptide from the same precursor, prepro-MCH, as MCH, induce REM sleep at least in part through acting on the vlPAG
Title: Neuropeptide glutamic acid-isoleucine (NEI)-induced paradoxical sleep in rats.

MCH levels in the CSF, brain preproMCH and MCHR1 gene expression during paradoxical sleep deprivation, sleep rebound and chronic sleep restriction were studied in rats.
Title: MCH levels in the CSF, brain preproMCH and MCHR1 gene expression during paradoxical sleep deprivation, sleep rebound and chronic sleep restriction.

modulation of mPOA function by MCH may contribute to the weaning of maternal responsiveness characteristic of the late postpartum period.
Title: Melanin-concentrating hormone in the medial preoptic area reduces active components of maternal behavior in rats.

Results indicate that melanin-concentrating hormone and orexin peptides play physiologically distinct roles in feeding behavior.
Title: Expression of phosphorylated cyclic AMP response element-binding protein in melanin-concentrating hormone neurons and orexin neurons in male and female rats during ad-libitum feeding.

MCH participates not only in the regulation of glucose and sleep-wake homeostasis but also the vigilance-state dependent regulation of the central hypercapnic chemoreflex and respiratory control.
Title: The role of melanin concentrating hormone (MCH) in the central chemoreflex: a knockdown study by siRNA in the lateral hypothalamus in rats.

The following Pmch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pmch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00487	NM_012625.1 Latest version!	Rattus norvegicus pro-melanin-concentrating hormone (Pmch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pmch ( NM_012625.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036757.1 Rattus norvegicus pro-melanin-concentrating hormone (Pmch), mRNA.

Price & Availability↑

CloneID	ORa00487
Clone ID Related Accession (Same CDS sequence)	NM_012625.1
Accession Version	NM_012625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	pro-MCH precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M62641.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ214578.1, M29712.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595, SAMN02642596 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAAGA TGAGCCTCTC TTCCTACATG TTAATGCTGG CCTTTTCTTT GTTTTCTCAC 
GGCATTTTAC TTTCGGCCTC CAAGTCCATC AGGAACGTAG AAGACGACAT AGTATTTAAT 
ACATTCAGGA TGGGGAAAGC CTTTCAGAAG GAAGATACCG CAGAAAGATC GGTTGTTGCT 
CCTTCTCTGG AAGGATACAA AAATGATGAG AGCGGCTTCA TGAAGGATGA CGATGACAAG 
ACCACAAAGA ACACAGGCTC CAAGCAGAAT CTCGTAACTC ACGGTCTGCC CCTCAGTCTG 
GCTGTAAAAC CTTACCTCGC TCTGAAAGGA CCAGCAGTCT TCCCAGCTGA GAATGGAGTT 
CAGAATACTG AGTCCACACA GGAAAAGAGG GAAATTGGGG ATGAAGAAAA CTCAGCTAAA 
TTTCCCATAG GAAGGAGAGA TTTTGACATG CTCAGGTGTA TGCTGGGACG AGTCTACCGA 
CCCTGTTGGC AAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036757.1
CDS	57..554
Translation	MAKMSLSSYMLMLAFSLFSHGILLSASKSIRNVEDDIVFNTFRMGKAFQKEDTAERSVVAPSLEGYKNDESGFMKDDDDKTTKNTGSKQNLVTHGLPLSLAVKPYLALKGPAVFPAENGVQNTESTQEKREIGDEENSAKFPIGRRDFDMLRCMLGRVYRPCWQV

Target ORF information:

RefSeq Version	NM_012625.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pro-melanin-concentrating hormone (Pmch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012625.1

ORF Insert Sequence:

ATGGCGAAGA TGAGCCTCTC TTCCTACATG TTAATGCTGG CCTTTTCTTT GTTTTCTCAC 
GGCATTTTAC TTTCGGCCTC CAAGTCCATC AGGAACGTAG AAGACGACAT AGTATTTAAT 
ACATTCAGGA TGGGGAAAGC CTTTCAGAAG GAAGATACCG CAGAAAGATC GGTTGTTGCT 
CCTTCTCTGG AAGGATACAA AAATGATGAG AGCGGCTTCA TGAAGGATGA CGATGACAAG 
ACCACAAAGA ACACAGGCTC CAAGCAGAAT CTCGTAACTC ACGGTCTGCC CCTCAGTCTG 
GCTGTAAAAC CTTACCTCGC TCTGAAAGGA CCAGCAGTCT TCCCAGCTGA GAATGGAGTT 
CAGAATACTG AGTCCACACA GGAAAAGAGG GAAATTGGGG ATGAAGAAAA CTCAGCTAAA 
TTTCCCATAG GAAGGAGAGA TTTTGACATG CTCAGGTGTA TGCTGGGACG AGTCTACCGA 
CCCTGTTGGC AAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Litter size determines the number of melanin-concentrating hormone neurons in the medial preoptic area of Sprague Dawley lactating dams.
Physiology & behavior18175-79(2017 Nov)
Ferreira JGP,Duarte JCG,Diniz GB,Bittencourt JC

Neuropeptide glutamic acid-isoleucine (NEI)-induced paradoxical sleep in rats.
Peptides8728-33(2017 Jan)
Fujimoto M,Fukuda S,Sakamoto H,Takata J,Sawamura S

MCH levels in the CSF, brain preproMCH and MCHR1 gene expression during paradoxical sleep deprivation, sleep rebound and chronic sleep restriction.
Peptides749-15(2015 Dec)
Dias Abdo Agamme AL,Aguilar Calegare BF,Fernandes L,Costa A,Lagos P,Torterolo P,D'Almeida V

Melanin-concentrating hormone in the medial preoptic area reduces active components of maternal behavior in rats.
Peptides5820-5(2014 Aug)
Benedetto L,Pereira M,Ferreira A,Torterolo P

The role of melanin concentrating hormone (MCH) in the central chemoreflex: a knockdown study by siRNA in the lateral hypothalamus in rats.
PloS one9(8)e103585(2014)
Li N,Nattie E,Li A

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Pmch cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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