Plcb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plcb1
Entrez Gene ID	24654
Full Name	phospholipase C beta 1
Synonyms	PLC'1,PLCbeta1,Phosphb,RATPHOSPHB
General protein information	Preferred Names phospholipase C beta 1 Names 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 PLC-154 PLC-I PLC-beta-1 Phospholipase C-beta1 phosphoinositide phospholipase C phosphoinositide phospholipase C-beta-1 phospholipase C, beta 1 (phosphoinositide-specific) phospholipase C-I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077641.1	NP_001071109.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
XM_017591466.1	XP_017446955.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
XM_017591467.1	XP_017446956.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X2

Pathways from BioSystems

Homologies

	PLCB1	XP_001116017.1
Mus musculus (house mouse)	Plcb1	NP_001139302.1
Canis lupus familiaris (dog)	PLCB1	XP_005634858.1
Bos taurus (cattle)	PLCB1	NP_777242.1
Gallus gallus (chicken)	PLCB1	XP_425261.3
Drosophila melanogaster (fruit fly)	Plc21C	NP_995604.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009115	XP_319865.4
Homo sapiens (human)	PLCB1	NP_056007.1
Pan troglodytes (chimpanzee)	PLCB1	XP_001170216.3
Rattus norvegicus (Norway rat)	Plcb1	NP_001071109.1

Gene Ontology
Bibliography

Related articles in PubMed

Phospholipase C?-TRAX Association Is Required for PC12 Cell Differentiation.
Garwain O, Scarlata S
The Journal of biological chemistry291(44)22970-22976(2016 10)

MLP and CARP are linked to chronic PKC? signalling in dilated cardiomyopathy.
Lange S, Gehmlich K, Lun AS, Blondelle J, Hooper C, Dalton ND, Alvarez EA, Zhang X, Bang ML, Abassi YA, Dos Remedios CG, Peterson KL, Chen J, Ehler E
Nature communications712120(2016 Jun)

Oxidative stress contributes to the enhanced expression of Gq?/PLC?1 proteins and hypertrophy of VSMC from SHR: role of growth factor receptor transactivation.
Atef ME, Anand-Srivastava MB
American journal of physiology. Heart and circulatory physiology310(5)H608-18(2016 Mar)

Role of PKC? in Enhanced Expression of Gq?/PLC?1 Proteins and VSMC Hypertrophy in Spontaneously Hypertensive Rats.
Atef ME, Anand-Srivastava MB
PloS one11(7)e0157955(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PLCbeta through its ability to bind TRAX plays a key role in switching PC12 cells to their differentiated state.
Title: Phospholipase C?-TRAX Association Is Required for PC12 Cell Differentiation.

Enhanced oxidative stress in VSMCs from SHRs activates c-Src, growth factor receptors and MAPK signaling, contributing to enhanced expression of Gqalpha/PLCbeta1 proteins and VSMC hypertrophy.
Title: Oxidative stress contributes to the enhanced expression of Gq?/PLC?1 proteins and hypertrophy of VSMC from SHR: role of growth factor receptor transactivation.

down-regulation of gamma-synuclein reduces the protein level of PLCbeta but increases the transcript level over 40 fold
Title: The Breast Cancer Susceptibility Gene Product (?-Synuclein) Alters Cell Behavior through its [corrected] Interaction with Phospholipase C?.

Enhanced activation of protein kinase C delta (PKCdelta) in vascular smooth muscle cell (VSMC) from hypertensive rats (SHR) increases the expression of G alpha protein q (Gqalpha) and phospholipase C beta1 (PLCbeta1) proteins and results in VSMC hypertrophy.
Title: Role of PKC? in Enhanced Expression of Gq?/PLC?1 Proteins and VSMC Hypertrophy in Spontaneously Hypertensive Rats.

Results suggest that synergistic endocannabinoid mobilization in hippocampal CA1 neurons is greater in juveniles than in neonates, and that this may result from increases in the mGluR5-PLCbeta1 endocannabinoid pathway
Title: Developmental increase in hippocampal endocannabinoid mobilization: role of metabotropic glutamate receptor subtype 5 and phospholipase C.

The following Plcb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plcb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa60263	XM_017591467.1 Latest version!	Rattus norvegicus phospholipase C beta 1 (Plcb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa42600	NM_001077641.1 Latest version!	Rattus norvegicus phospholipase C beta 1 (Plcb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$895.30 $1279.00
ORa42942	XM_017591466.1 Latest version!	Rattus norvegicus phospholipase C beta 1 (Plcb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plcb1 ( XM_017591467.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446956.1 Rattus norvegicus phospholipase C beta 1 (Plcb1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60263
Clone ID Related Accession (Same CDS sequence)	XM_017591467.1
Accession Version	XM_017591467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT AGTTACTCCA 
ATTATTTTGA GGACCGATCC TCAGGGATTT TTCTTTTACT GGACAGATCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTCGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTACG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGGCCAGAC CTGGTGAATA TCTCCCACCT GAATCTTGTG 
GCTTTTCAAG AGGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTTCAGGTGA CCCCAGAAGG GCGCATTCCT CTTAAAAACA TCTATCGACT GTTTTCGGCA 
GACCGGAAGC GAGTGGAAAC TGCGCTAGAG GCCTGTAGTC TTCCATCGTC AAGGAACGAT 
TCCATCCCTC AAGAGGACTT TACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTTTGT 
CCCCGACCTG AAATTGATAA CATCTTCTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTT 
ACTGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTGAATGAA 
ATACTTTACC CACCTCTGAA GCAAGAGCAG GTCCAAGTGT TGATTGAGAA GTACGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG CAGGACCGCT GAGGAAGAGC CTGTCATCAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACTTCTC CTTTTCCCAT CCTCCTTTCC TTTGAGAACC ATGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT TATGGAGCCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCA CACAAGTCGT CAGAGGGAAG TGGTAAAAAG 
AAGCTCTCTG AGCAAGCTTC CAACACGTAC AGCGACTCTT CCAGCGTGTT CGAGCCTTCG 
TCTCCGGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA TGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACTGCT GGCAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAC TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTCGA ACAACTCACG 
AAGTCTCCAG TTGAATTTGT CGAATACAAC AAGATGCAGC TTAGCAGGAT ATATCCCAAA 
GGAACACGCG TGGACTCATC CAACTACATG CCTCAGCTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAC 
GAATACAATG GGAAGAGTGG CTACAGGCTG AAGCCAGAGT TCATGAGGAG GCCAGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGAAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ATCCTGTCTG GGAAGAAGAG CCAATTGTAT TCAAAAAGGT AGTTCTGCCT 
TCTCTGGCCT GTTTAAGGAT AGCAGCATAT GAGGAAGGAG GCAAATTTAT TGGCCACCGG 
ATCTTGCCTG TGCAAGCAAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGATGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA CTATGTCCCA 
GACACGTATG CAGATGTAAT TGAAGCATTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGTT GGCTGCATTG ACACTGGAGG ATGAAGAGGA AGTCAAGAAG 
GAGGCTGACC CCGGGGAAAC GTCATCCGAG GCTCCAAGTG AAACCAGGAC AACTCCAGCA 
GAGAATGGGG TGAATCACAC CGCAACCCTT GCACCCAAGC CACCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCCGCCAAAA CAGAGGATCT GATTCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAGACCATT GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAGCACTA CAAAGAAATG AAAGACCTGG TCAAGAGACA CCACAAGAAA 
ACCACCGAGC TCATCAAGGA GCATACCACC AAGTACAATG AGATTCAGAA TGACTACTTG 
AGAAGGAGGG CAGCCTTGGA AAAGTCCGCC AAAAAGGATA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCAG ATCATGGCTC ATCCGCCATT GAGCAAGACC TCGCGGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGTGA GAAGTACCAG AAGCGGGAGC ACATTAAATT GCTCATTCAG 
AAGTTGACAG ATGTTGCTGA AGAGTGTCAG AACAATCAGT TGAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA GGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TTGTGGAAAA ACACAAGGAG ATCCGCCAGC AGATCCTGGA TGAGAAGCCC 
AAGGGGGAAG GCTCCTCCTC AGTCTTGTCG GAAAGTTGCC ATGAGGATCC CTCTGTTCCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446956.1
CDS	359..3880
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFETSKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTATLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKGEGSSSVLSESCHEDPSVPPNFTPPNPQALKW

Target ORF information:

RefSeq Version	XM_017591467.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C beta 1 (Plcb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591467.1

ORF Insert Sequence:

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT AGTTACTCCA 
ATTATTTTGA GGACCGATCC TCAGGGATTT TTCTTTTACT GGACAGATCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTCGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTACG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGGCCAGAC CTGGTGAATA TCTCCCACCT GAATCTTGTG 
GCTTTTCAAG AGGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTTCAGGTGA CCCCAGAAGG GCGCATTCCT CTTAAAAACA TCTATCGACT GTTTTCGGCA 
GACCGGAAGC GAGTGGAAAC TGCGCTAGAG GCCTGTAGTC TTCCATCGTC AAGGAACGAT 
TCCATCCCTC AAGAGGACTT TACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTTTGT 
CCCCGACCTG AAATTGATAA CATCTTCTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTT 
ACTGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTGAATGAA 
ATACTTTACC CACCTCTGAA GCAAGAGCAG GTCCAAGTGT TGATTGAGAA GTACGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG CAGGACCGCT GAGGAAGAGC CTGTCATCAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACTTCTC CTTTTCCCAT CCTCCTTTCC TTTGAGAACC ATGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT TATGGAGCCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCA CACAAGTCGT CAGAGGGAAG TGGTAAAAAG 
AAGCTCTCTG AGCAAGCTTC CAACACGTAC AGCGACTCTT CCAGCGTGTT CGAGCCTTCG 
TCTCCGGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA TGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACTGCT GGCAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAC TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTCGA ACAACTCACG 
AAGTCTCCAG TTGAATTTGT CGAATACAAC AAGATGCAGC TTAGCAGGAT ATATCCCAAA 
GGAACACGCG TGGACTCATC CAACTACATG CCTCAGCTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAC 
GAATACAATG GGAAGAGTGG CTACAGGCTG AAGCCAGAGT TCATGAGGAG GCCAGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGAAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ATCCTGTCTG GGAAGAAGAG CCAATTGTAT TCAAAAAGGT AGTTCTGCCT 
TCTCTGGCCT GTTTAAGGAT AGCAGCATAT GAGGAAGGAG GCAAATTTAT TGGCCACCGG 
ATCTTGCCTG TGCAAGCAAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGATGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA CTATGTCCCA 
GACACGTATG CAGATGTAAT TGAAGCATTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGTT GGCTGCATTG ACACTGGAGG ATGAAGAGGA AGTCAAGAAG 
GAGGCTGACC CCGGGGAAAC GTCATCCGAG GCTCCAAGTG AAACCAGGAC AACTCCAGCA 
GAGAATGGGG TGAATCACAC CGCAACCCTT GCACCCAAGC CACCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCCGCCAAAA CAGAGGATCT GATTCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAGACCATT GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAGCACTA CAAAGAAATG AAAGACCTGG TCAAGAGACA CCACAAGAAA 
ACCACCGAGC TCATCAAGGA GCATACCACC AAGTACAATG AGATTCAGAA TGACTACTTG 
AGAAGGAGGG CAGCCTTGGA AAAGTCCGCC AAAAAGGATA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCAG ATCATGGCTC ATCCGCCATT GAGCAAGACC TCGCGGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGTGA GAAGTACCAG AAGCGGGAGC ACATTAAATT GCTCATTCAG 
AAGTTGACAG ATGTTGCTGA AGAGTGTCAG AACAATCAGT TGAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA GGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TTGTGGAAAA ACACAAGGAG ATCCGCCAGC AGATCCTGGA TGAGAAGCCC 
AAGGGGGAAG GCTCCTCCTC AGTCTTGTCG GAAAGTTGCC ATGAGGATCC CTCTGTTCCC 
CCCAACTTTA CTCCCCCCAA CCCTCAAGCT CTCAAGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb1 ( NM_001077641.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001071109.1 Rattus norvegicus phospholipase C beta 1 (Plcb1), mRNA.

Price & Availability↑

CloneID	ORa42600
Clone ID Related Accession (Same CDS sequence)	NM_001077641.1
Accession Version	NM_001077641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M20636.1. On Nov 10, 2006 this sequence version replaced XM_342524.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M20636.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT AGTTACTCCA 
ATTATTTTGA GGACCGATCC TCAGGGATTT TTCTTTTACT GGACAGATCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTCGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTACG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGGCCAGAC CTGGTGAATA TCTCCCACCT GAATCTTGTG 
GCTTTTCAAG AGGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTTCAGGTGA CCCCAGAAGG GCGCATTCCT CTTAAAAACA TCTATCGACT GTTTTCGGCA 
GACCGGAAGC GAGTGGAAAC TGCGCTAGAG GCTTGTAGTC TTCCATCGTC AAGGAACGAT 
TCCATCCCTC AAGAGGACTT TACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTTTGT 
CCCCGACCTG AAATTGATAA CATCTTCTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTT 
ACTGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTGAATGAA 
ATACTTTACC CACCTCTGAA GCAAGAGCAG GTCCAAGTGT TGATTGAGAA GTACGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG CAGGACCGCT GAGGAAGAGC CTGTCATCAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACTTCTC CTTTTCCCAT CCTCCTTTCC TTTGAGAACC ATGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT TATGGAGCCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCA CACAAGTCGT CAGAGGGAAG TGGTAAAAAG 
AAGCTCTCTG AGCAAGCTTC CAACACGTAC AGCGACTCTT CCAGCGTGTT CGAGCCTTCG 
TCTCCGGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA TGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACTGCT GGCAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAC TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTCGA ACAACTCACG 
AAGTCTCCAG TTGAATTTGT CGAATACAAC AAGATGCAGC TTAGCAGGAT ATATCCCAAA 
GGAACACGCG TGGACTCATC CAACTACATG CCTCAGCTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAC 
GAATACAATG GGAAGAGTGG CTACAGGCTG AAGCCAGAGT TCATGAGGAG GCCAGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGAAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ATCCTGTCTG GGAAGAAGAG CCAATTGTAT TCAAAAAGGT AGTTCTGCCT 
TCTCTGGCCT GTTTAAGGAT AGCAGCATAT GAGGAAGGAG GCAAATTTAT TGGCCACCGG 
ATCTTGCCTG TGCAAGCAAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGATGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA CTATGTCCCA 
GACACGTATG CAGATGTAAT TGAAGCATTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGTT GGCTGCATTG ACACTGGAGG ATGAAGAGGA AGTCAAGAAG 
GAGGCTGACC CCGGGGAAAC GTCATCCGAG GCTCCAAGTG AAACCAGGAC AACTCCAGCA 
GAGAATGGGG TGAATCACAC CGCAACCCTT GCACCCAAGC CACCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCCGCCAAAA CAGAGGATCT GATTCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAGACCATT GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAGCACTA CAAAGAAATG AAAGACCTGG TCAAGAGACA CCACAAGAAA 
ACCACCGAGC TCATCAAGGA GCATACCACC AAGTACAATG AGATTCAGAA TGACTACTTG 
AGAAGGAGGG CAGCCTTGGA AAAGTCCGCC AAAAAGGATA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCAG ATCATGGCTC ATCCGCCATT GAGCAAGACC TCGCGGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGTGA GAAGTACCAG AAGCGGGAGC ACATTAAATT GCTCATTCAG 
AAGTTGACAG ATGTTGCTGA AGAGTGTCAG AACAATCAGT TGAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA GGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TTGTGGAAAA ACACAAGGAG ATCCGCCAGC AGATCCTGGA TGAGAAGCCC 
AAGCTGCAGA TGGAGCTGGA GCAAGAATAC CAAGACAAGT TCAAAAGACT GCCCCTGGAG 
ATTCTGGAGT TTGTACAGGA AGCCATGAAA GGGAAGGTTA GTGAGGACAG CAATCACGGC 
TCTGCCCCTC CCTCGCTGGC CTCAGACCCT GCTAAGGTGA ACCTCAAGTC TCCCTCCAGT 
GAGGAGGTAC AAGGAGAGAA CGCGGGAAGA GAGTTTGATA CTCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071109.1
CDS	212..3862
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFETSKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTATLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKLQMELEQEYQDKFKRLPLEILEFVQEAMKGKVSEDSNHGSAPPSLASDPAKVNLKSPSSEEVQGENAGREFDTPL

Target ORF information:

RefSeq Version	NM_001077641.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C beta 1 (Plcb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077641.1

ORF Insert Sequence:

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT AGTTACTCCA 
ATTATTTTGA GGACCGATCC TCAGGGATTT TTCTTTTACT GGACAGATCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTCGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTACG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGGCCAGAC CTGGTGAATA TCTCCCACCT GAATCTTGTG 
GCTTTTCAAG AGGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTTCAGGTGA CCCCAGAAGG GCGCATTCCT CTTAAAAACA TCTATCGACT GTTTTCGGCA 
GACCGGAAGC GAGTGGAAAC TGCGCTAGAG GCTTGTAGTC TTCCATCGTC AAGGAACGAT 
TCCATCCCTC AAGAGGACTT TACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTTTGT 
CCCCGACCTG AAATTGATAA CATCTTCTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTT 
ACTGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTGAATGAA 
ATACTTTACC CACCTCTGAA GCAAGAGCAG GTCCAAGTGT TGATTGAGAA GTACGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG CAGGACCGCT GAGGAAGAGC CTGTCATCAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACTTCTC CTTTTCCCAT CCTCCTTTCC TTTGAGAACC ATGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT TATGGAGCCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCA CACAAGTCGT CAGAGGGAAG TGGTAAAAAG 
AAGCTCTCTG AGCAAGCTTC CAACACGTAC AGCGACTCTT CCAGCGTGTT CGAGCCTTCG 
TCTCCGGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA TGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACTGCT GGCAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAC TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTCGA ACAACTCACG 
AAGTCTCCAG TTGAATTTGT CGAATACAAC AAGATGCAGC TTAGCAGGAT ATATCCCAAA 
GGAACACGCG TGGACTCATC CAACTACATG CCTCAGCTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAC 
GAATACAATG GGAAGAGTGG CTACAGGCTG AAGCCAGAGT TCATGAGGAG GCCAGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGAAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ATCCTGTCTG GGAAGAAGAG CCAATTGTAT TCAAAAAGGT AGTTCTGCCT 
TCTCTGGCCT GTTTAAGGAT AGCAGCATAT GAGGAAGGAG GCAAATTTAT TGGCCACCGG 
ATCTTGCCTG TGCAAGCAAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGATGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA CTATGTCCCA 
GACACGTATG CAGATGTAAT TGAAGCATTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGTT GGCTGCATTG ACACTGGAGG ATGAAGAGGA AGTCAAGAAG 
GAGGCTGACC CCGGGGAAAC GTCATCCGAG GCTCCAAGTG AAACCAGGAC AACTCCAGCA 
GAGAATGGGG TGAATCACAC CGCAACCCTT GCACCCAAGC CACCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCCGCCAAAA CAGAGGATCT GATTCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAGACCATT GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAGCACTA CAAAGAAATG AAAGACCTGG TCAAGAGACA CCACAAGAAA 
ACCACCGAGC TCATCAAGGA GCATACCACC AAGTACAATG AGATTCAGAA TGACTACTTG 
AGAAGGAGGG CAGCCTTGGA AAAGTCCGCC AAAAAGGATA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCAG ATCATGGCTC ATCCGCCATT GAGCAAGACC TCGCGGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGTGA GAAGTACCAG AAGCGGGAGC ACATTAAATT GCTCATTCAG 
AAGTTGACAG ATGTTGCTGA AGAGTGTCAG AACAATCAGT TGAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA GGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TTGTGGAAAA ACACAAGGAG ATCCGCCAGC AGATCCTGGA TGAGAAGCCC 
AAGCTGCAGA TGGAGCTGGA GCAAGAATAC CAAGACAAGT TCAAAAGACT GCCCCTGGAG 
ATTCTGGAGT TTGTACAGGA AGCCATGAAA GGGAAGGTTA GTGAGGACAG CAATCACGGC 
TCTGCCCCTC CCTCGCTGGC CTCAGACCCT GCTAAGGTGA ACCTCAAGTC TCCCTCCAGT 
GAGGAGGTAC AAGGAGAGAA CGCGGGAAGA GAGTTTGATA CTCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Plcb1 ( XM_017591466.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446955.1 Rattus norvegicus phospholipase C beta 1 (Plcb1), transcript variant X1, mRNA.

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CloneID	ORa42942
Clone ID Related Accession (Same CDS sequence)	XM_017591466.1
Accession Version	XM_017591466.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT AGTTACTCCA 
ATTATTTTGA GGACCGATCC TCAGGGATTT TTCTTTTACT GGACAGATCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTCGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTACG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGGCCAGAC CTGGTGAATA TCTCCCACCT GAATCTTGTG 
GCTTTTCAAG AGGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTTCAGGTGA CCCCAGAAGG GCGCATTCCT CTTAAAAACA TCTATCGACT GTTTTCGGCA 
GACCGGAAGC GAGTGGAAAC TGCGCTAGAG GCCTGTAGTC TTCCATCGTC AAGGAACGAT 
TCCATCCCTC AAGAGGACTT TACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTTTGT 
CCCCGACCTG AAATTGATAA CATCTTCTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTT 
ACTGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTGAATGAA 
ATACTTTACC CACCTCTGAA GCAAGAGCAG GTCCAAGTGT TGATTGAGAA GTACGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG CAGGACCGCT GAGGAAGAGC CTGTCATCAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACTTCTC CTTTTCCCAT CCTCCTTTCC TTTGAGAACC ATGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT TATGGAGCCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCA CACAAGTCGT CAGAGGGAAG TGGTAAAAAG 
AAGCTCTCTG AGCAAGCTTC CAACACGTAC AGCGACTCTT CCAGCGTGTT CGAGCCTTCG 
TCTCCGGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA TGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACTGCT GGCAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAC TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTCGA ACAACTCACG 
AAGTCTCCAG TTGAATTTGT CGAATACAAC AAGATGCAGC TTAGCAGGAT ATATCCCAAA 
GGAACACGCG TGGACTCATC CAACTACATG CCTCAGCTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAC 
GAATACAATG GGAAGAGTGG CTACAGGCTG AAGCCAGAGT TCATGAGGAG GCCAGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGAAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ATCCTGTCTG GGAAGAAGAG CCAATTGTAT TCAAAAAGGT AGTTCTGCCT 
TCTCTGGCCT GTTTAAGGAT AGCAGCATAT GAGGAAGGAG GCAAATTTAT TGGCCACCGG 
ATCTTGCCTG TGCAAGCAAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGATGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA CTATGTCCCA 
GACACGTATG CAGATGTAAT TGAAGCATTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGTT GGCTGCATTG ACACTGGAGG ATGAAGAGGA AGTCAAGAAG 
GAGGCTGACC CCGGGGAAAC GTCATCCGAG GCTCCAAGTG AAACCAGGAC AACTCCAGCA 
GAGAATGGGG TGAATCACAC CGCAACCCTT GCACCCAAGC CACCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCCGCCAAAA CAGAGGATCT GATTCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAGACCATT GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAGCACTA CAAAGAAATG AAAGACCTGG TCAAGAGACA CCACAAGAAA 
ACCACCGAGC TCATCAAGGA GCATACCACC AAGTACAATG AGATTCAGAA TGACTACTTG 
AGAAGGAGGG CAGCCTTGGA AAAGTCCGCC AAAAAGGATA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCAG ATCATGGCTC ATCCGCCATT GAGCAAGACC TCGCGGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGTGA GAAGTACCAG AAGCGGGAGC ACATTAAATT GCTCATTCAG 
AAGTTGACAG ATGTTGCTGA AGAGTGTCAG AACAATCAGT TGAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA GGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TTGTGGAAAA ACACAAGGAG ATCCGCCAGC AGATCCTGGA TGAGAAGCCC 
AAGCTGCAGA TGGAGCTGGA GCAAGAATAC CAAGACAAGT TCAAAAGACT GCCCCTGGAG 
ATTCTGGAGT TTGTACAGGA AGCCATGAAA GGGAAGGTTA GTGAGGACAG CAATCACGGC 
TCTGCCCCTC CCTCGCTGGC CTCAGACCCT GCTAAGGTGA ACCTCAAGTC TCCCTCCAGT 
GAGGAGGTAC AAGGAGAGAA CGCGGGAAGA GAGTTTGATA CTCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446955.1
CDS	359..4009
Translation	MAGAQPGVHALQLKPVCVSDSLKKGTKFVKWDDDSTIVTPIILRTDPQGFFFYWTDQNKETELLDLSLVKDARCGKHAKAPKDPKLRELLDVGNIGHLEQRMITVVYGPDLVNISHLNLVAFQEEVAKEWTNEVFSLATNLLAQNMSRDAFLEKAYTKLKLQVTPEGRIPLKNIYRLFSADRKRVETALEACSLPSSRNDSIPQEDFTPDVYRVFLNNLCPRPEIDNIFSEFGAKSKPYLTVDQMMDFINLKQRDPRLNEILYPPLKQEQVQVLIEKYEPNSSLAKKGQMSVDGFMRYLSGEENGVVSPEKLDLNEDMSQPLSHYFINSSHNTYLTAGQLAGNSSVEMYRQVLLSGCRCVELDCWKGRTAEEEPVITHGFTMTTEISFKEVIEAIAECAFKTSPFPILLSFENHVDSPKQQAKMAEYCRLIFGDALLMEPLEKYPLESGVPLPSPMDLMYKILVKNKKKSHKSSEGSGKKKLSEQASNTYSDSSSVFEPSSPGAGEADTESDDDDDDDDCKKSSMDEGTAGSEAMATEEMSNLVNYIQPVKFESFETSKKRNKSFEMSSFVETKGLEQLTKSPVEFVEYNKMQLSRIYPKGTRVDSSNYMPQLFWNAGCQMVALNFQTVDLAMQINMGMYEYNGKSGYRLKPEFMRRPDKHFDPFTEGIVDGIVANTLSVKIISGQFLSDKKVGTYVEVDMFGLPVDTRRKAFKTKTSQGNAVNPVWEEEPIVFKKVVLPSLACLRIAAYEEGGKFIGHRILPVQAIRPGYHYICLRNERNQPLMLPAVFVYIEVKDYVPDTYADVIEALSNPIRYVNLMEQRAKQLAALTLEDEEEVKKEADPGETSSEAPSETRTTPAENGVNHTATLAPKPPSQAPHSQPAPGSVKAPAKTEDLIQSVLTEVEAQTIEELKQQKSFVKLQKKHYKEMKDLVKRHHKKTTELIKEHTTKYNEIQNDYLRRRAALEKSAKKDSKKKSEPSSPDHGSSAIEQDLAALDAEMTQKLIDLKDKQQQQLLNLRQEQYYSEKYQKREHIKLLIQKLTDVAEECQNNQLKKLKEICEKEKKELKKKMDKKRQEKITEAKSKDKSQMEEEKTEMIRSYIQEVVQYIKRLEEAQSKRQEKLVEKHKEIRQQILDEKPKLQMELEQEYQDKFKRLPLEILEFVQEAMKGKVSEDSNHGSAPPSLASDPAKVNLKSPSSEEVQGENAGREFDTPL

Target ORF information:

RefSeq Version	XM_017591466.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase C beta 1 (Plcb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591466.1

ORF Insert Sequence:

ATGGCTGGGG CTCAGCCCGG AGTGCACGCC TTGCAACTCA AGCCCGTGTG CGTGTCCGAC 
AGCCTCAAGA AGGGCACCAA ATTCGTCAAG TGGGATGATG ACTCCACTAT AGTTACTCCA 
ATTATTTTGA GGACCGATCC TCAGGGATTT TTCTTTTACT GGACAGATCA GAATAAGGAG 
ACGGAGCTGT TAGATCTCAG CCTCGTCAAG GATGCCAGGT GTGGGAAGCA CGCCAAAGCT 
CCCAAGGACC CCAAGTTACG TGAACTTCTG GATGTTGGGA ACATCGGACA CTTGGAACAG 
CGCATGATAA CTGTGGTGTA TGGGCCAGAC CTGGTGAATA TCTCCCACCT GAATCTTGTG 
GCTTTTCAAG AGGAAGTGGC CAAGGAATGG ACAAATGAGG TTTTCAGTTT GGCAACAAAC 
CTGCTGGCTC AGAACATGTC CAGGGACGCA TTTCTGGAGA AAGCATATAC TAAGCTCAAG 
CTTCAGGTGA CCCCAGAAGG GCGCATTCCT CTTAAAAACA TCTATCGACT GTTTTCGGCA 
GACCGGAAGC GAGTGGAAAC TGCGCTAGAG GCCTGTAGTC TTCCATCGTC AAGGAACGAT 
TCCATCCCTC AAGAGGACTT TACTCCAGAT GTATACAGAG TTTTCCTGAA CAATCTTTGT 
CCCCGACCTG AAATTGATAA CATCTTCTCT GAATTTGGTG CCAAAAGCAA ACCGTACCTT 
ACTGTTGATC AGATGATGGA TTTTATCAAC CTTAAGCAGA GAGATCCCCG GCTGAATGAA 
ATACTTTACC CACCTCTGAA GCAAGAGCAG GTCCAAGTGT TGATTGAGAA GTACGAGCCC 
AACAGCAGCC TCGCCAAGAA AGGGCAGATG TCAGTGGATG GATTCATGCG CTACCTGAGC 
GGAGAAGAAA ATGGAGTCGT TTCACCTGAG AAACTGGATT TGAACGAAGA CATGTCTCAG 
CCCCTGTCTC ACTATTTCAT CAATTCCTCA CACAACACCT ACCTCACAGC TGGCCAGTTG 
GCTGGGAACT CGTCTGTAGA GATGTATCGC CAGGTGCTTC TGTCTGGATG TCGCTGTGTG 
GAGCTGGACT GCTGGAAGGG CAGGACCGCT GAGGAAGAGC CTGTCATCAC CCATGGATTC 
ACCATGACAA CAGAAATATC CTTCAAGGAA GTCATAGAAG CCATCGCAGA GTGTGCGTTC 
AAGACTTCTC CTTTTCCCAT CCTCCTTTCC TTTGAGAACC ATGTGGATTC CCCGAAGCAA 
CAAGCCAAGA TGGCCGAGTA TTGCCGATTA ATCTTTGGTG ATGCCCTCCT TATGGAGCCA 
CTGGAAAAAT ACCCACTGGA ATCTGGGGTA CCTCTTCCAA GCCCTATGGA TTTAATGTAT 
AAAATCTTGG TGAAAAACAA GAAGAAGTCA CACAAGTCGT CAGAGGGAAG TGGTAAAAAG 
AAGCTCTCTG AGCAAGCTTC CAACACGTAC AGCGACTCTT CCAGCGTGTT CGAGCCTTCG 
TCTCCGGGAG CTGGGGAAGC AGATACGGAG AGTGATGACG ATGACGACGA TGATGACTGT 
AAAAAGTCTT CCATGGATGA GGGGACTGCT GGCAGCGAGG CCATGGCCAC AGAAGAGATG 
TCTAACCTGG TGAACTATAT TCAGCCTGTC AAGTTTGAGT CCTTTGAAAC TTCAAAAAAA 
AGAAATAAAA GCTTTGAAAT GTCTTCCTTC GTGGAAACCA AAGGACTCGA ACAACTCACG 
AAGTCTCCAG TTGAATTTGT CGAATACAAC AAGATGCAGC TTAGCAGGAT ATATCCCAAA 
GGAACACGCG TGGACTCATC CAACTACATG CCTCAGCTCT TCTGGAATGC TGGCTGTCAG 
ATGGTGGCGC TCAACTTCCA GACAGTGGAT CTAGCTATGC AGATAAACAT GGGCATGTAC 
GAATACAATG GGAAGAGTGG CTACAGGCTG AAGCCAGAGT TCATGAGGAG GCCAGACAAG 
CATTTTGATC CATTTACTGA AGGAATCGTA GATGGGATAG TGGCCAACAC TTTATCTGTT 
AAGATTATTT CAGGTCAGTT TCTCTCTGAT AAGAAAGTTG GGACTTATGT GGAAGTGGAT 
ATGTTTGGTT TGCCTGTGGA CACAAGAAGG AAGGCATTTA AAACCAAGAC ATCCCAAGGA 
AATGCTGTAA ATCCTGTCTG GGAAGAAGAG CCAATTGTAT TCAAAAAGGT AGTTCTGCCT 
TCTCTGGCCT GTTTAAGGAT AGCAGCATAT GAGGAAGGAG GCAAATTTAT TGGCCACCGG 
ATCTTGCCTG TGCAAGCAAT TCGGCCAGGC TATCACTACA TCTGCCTGCG GAATGAGAGG 
AACCAGCCCC TGATGCTGCC AGCTGTCTTT GTCTACATAG AAGTCAAAGA CTATGTCCCA 
GACACGTATG CAGATGTAAT TGAAGCATTA TCAAACCCAA TCCGATATGT CAATCTGATG 
GAACAGAGAG CTAAGCAGTT GGCTGCATTG ACACTGGAGG ATGAAGAGGA AGTCAAGAAG 
GAGGCTGACC CCGGGGAAAC GTCATCCGAG GCTCCAAGTG AAACCAGGAC AACTCCAGCA 
GAGAATGGGG TGAATCACAC CGCAACCCTT GCACCCAAGC CACCTTCCCA GGCTCCACAC 
AGCCAGCCTG CTCCAGGGTC TGTGAAGGCA CCCGCCAAAA CAGAGGATCT GATTCAGAGC 
GTGTTAACAG AAGTAGAGGC GCAGACCATT GAAGAGCTCA AGCAACAGAA ATCGTTCGTG 
AAACTTCAAA AGAAGCACTA CAAAGAAATG AAAGACCTGG TCAAGAGACA CCACAAGAAA 
ACCACCGAGC TCATCAAGGA GCATACCACC AAGTACAATG AGATTCAGAA TGACTACTTG 
AGAAGGAGGG CAGCCTTGGA AAAGTCCGCC AAAAAGGATA GCAAGAAGAA ATCTGAACCC 
AGCAGCCCAG ATCATGGCTC ATCCGCCATT GAGCAAGACC TCGCGGCCCT GGATGCAGAA 
ATGACTCAGA AGTTGATAGA CTTGAAAGAC AAGCAACAAC AGCAGCTGCT TAATCTTCGG 
CAAGAGCAGT ATTACAGTGA GAAGTACCAG AAGCGGGAGC ACATTAAATT GCTCATTCAG 
AAGTTGACAG ATGTTGCTGA AGAGTGTCAG AACAATCAGT TGAAGAAGCT GAAGGAAATC 
TGCGAGAAAG AGAAGAAGGA ATTAAAGAAG AAAATGGATA AGAAGAGGCA GGAGAAGATA 
ACAGAAGCCA AGTCCAAAGA CAAAAGCCAG ATGGAAGAGG AGAAGACAGA GATGATCCGA 
TCATACATCC AGGAGGTGGT GCAGTACATC AAGAGGTTAG AGGAAGCACA AAGTAAAAGA 
CAAGAAAAAC TTGTGGAAAA ACACAAGGAG ATCCGCCAGC AGATCCTGGA TGAGAAGCCC 
AAGCTGCAGA TGGAGCTGGA GCAAGAATAC CAAGACAAGT TCAAAAGACT GCCCCTGGAG 
ATTCTGGAGT TTGTACAGGA AGCCATGAAA GGGAAGGTTA GTGAGGACAG CAATCACGGC 
TCTGCCCCTC CCTCGCTGGC CTCAGACCCT GCTAAGGTGA ACCTCAAGTC TCCCTCCAGT 
GAGGAGGTAC AAGGAGAGAA CGCGGGAAGA GAGTTTGATA CTCCTCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phospholipase C?-TRAX Association Is Required for PC12 Cell Differentiation.
The Journal of biological chemistry291(44)22970-22976(2016 10)
Garwain O,Scarlata S

MLP and CARP are linked to chronic PKC? signalling in dilated cardiomyopathy.
Nature communications712120(2016 Jun)
Lange S,Gehmlich K,Lun AS,Blondelle J,Hooper C,Dalton ND,Alvarez EA,Zhang X,Bang ML,Abassi YA,Dos Remedios CG,Peterson KL,Chen J,Ehler E

Oxidative stress contributes to the enhanced expression of Gq?/PLC?1 proteins and hypertrophy of VSMC from SHR: role of growth factor receptor transactivation.
American journal of physiology. Heart and circulatory physiology310(5)H608-18(2016 Mar)
Atef ME,Anand-Srivastava MB

Role of PKC? in Enhanced Expression of Gq?/PLC?1 Proteins and VSMC Hypertrophy in Spontaneously Hypertensive Rats.
PloS one11(7)e0157955(2016)
Atef ME,Anand-Srivastava MB

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Plcb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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