Pklr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pklr
Entrez Gene ID	24651
Full Name	pyruvate kinase L/R
Synonyms	PK1,PKL,Pklg
General protein information	Preferred Names pyruvate kinase L/R Names pyruvate kinase PKLR L-PK pyruvate kinase isozymes L/R pyruvate kinase isozymes R/L pyruvate kinase, liver and RBC pyruvate kinase, liver and red blood cell
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	L-isozyme of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012624.3	NP_036756.3	pyruvate kinase PKLR
XM_006232593.3	XP_006232655.1	pyruvate kinase PKLR isoform X1
XM_017590631.1	XP_017446120.1	pyruvate kinase PKLR isoform X2

Pathways from BioSystems

Homologies

	PKLR	XP_001112902.2
Danio rerio (zebrafish)	pklr	NP_958446.1
Arabidopsis thaliana (thale cress)	AT3G55650	NP_191124.1
Homo sapiens (human)	PKLR	NP_000289.1
Canis lupus familiaris (dog)	PKLR	NP_001243191.1
Bos taurus (cattle)	PKLR	NP_001069644.1
Mus musculus (house mouse)	Pklr	NP_038659.2
Saccharomyces cerevisiae (baker's yeast)	PYK2	NP_014992.3
Arabidopsis thaliana (thale cress)	AT3G55810	NP_191140.1
Rattus norvegicus (Norway rat)	Pklr	NP_036756.3
Arabidopsis thaliana (thale cress)	AT3G25960	NP_189225.1
Pan troglodytes (chimpanzee)	PKLR	XP_524896.4
Arabidopsis thaliana (thale cress)	AT3G04050	NP_187055.1

Gene Ontology
Bibliography

Related articles in PubMed

Red blood cell PK deficiency: An update of PK-LR gene mutation database.
Canu G, De Bonis M, Minucci A, Capoluongo E
Blood cells, molecules & diseases57100-9(2016 Mar)

Probing L-pyruvate kinase regulatory phosphorylation site by mutagenesis.
Faustova I, Loog M, J?rv J
The protein journal31(7)592-7(2012 Oct)

Hypermethylation of hepatic glucokinase and L-type pyruvate kinase promoters in high-fat diet-induced obese rats.
Jiang M, Zhang Y, Liu M, Lan MS, Fei J, Fan W, Gao X, Lu D
Endocrinology152(4)1284-9(2011 Apr)

Malaria: looking for selection signatures in the human PKLR gene region.
Machado P, Pereira R, Rocha AM, Manco L, Fernandes N, Miranda J, Ribeiro L, do Ros?rio VE, Amorim A, Gusm?o L, Arez AP
British journal of haematology149(5)775-84(2010 Jun)

Rescue of pyruvate kinase deficiency in mice by gene therapy using the human isoenzyme.
Meza NW, Alonso-Ferrero ME, Navarro S, Quintana-Bustamante O, Valeri A, Garcia-Gomez M, Bueren JA, Bautista JM, Segovia JC
Molecular therapy : the journal of the American Society of Gene Therapy17(12)2000-9(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studied the effect of the point mutations around this phosphorylation site on the catalytic properties of L-pyruvate kinase.
Title: Probing L-pyruvate kinase regulatory phosphorylation site by mutagenesis.

Data report that DNA hypermethylation is correlated with a decline in hepatic glucokinase (Gck) and L-type pyruvate kinase (LPK) expression in high-fat diet-induced obese rats as compared with the control diet group.
Title: Hypermethylation of hepatic glucokinase and L-type pyruvate kinase promoters in high-fat diet-induced obese rats.

cAMP and glucose signaling converge on a complex containing ChREBP, HNF4alpha, and CBP, and cAMP acts by disrupting this transcriptional complex assembled by glucose-derived signals
Title: cAMP opposes the glucose-mediated induction of the L-PK gene by preventing the recruitment of a complex containing ChREBP, HNF4alpha, and CBP.

Data show that glucose regulates acetylation and methylation of various histone residues at the L-PK gene promoter.
Title: cAMP prevents glucose-mediated modifications of histone H3 and recruitment of the RNA polymerase II holoenzyme to the L-PK gene promoter.

A detailed analysis of the molecular events of the insulin-independent glucose response of the liver-type pyruvate kinase (L-PK) gene was performed.
Title: Detailed molecular analysis of the induction of the L-PK gene by glucose.

The following Pklr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pklr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12225	NM_012624.3 Latest version!	Rattus norvegicus pyruvate kinase L/R (Pklr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa24843	XM_006232593.3 Latest version!	Rattus norvegicus pyruvate kinase, liver and RBC (Pklr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa59626	XM_017590631.1 Latest version!	Rattus norvegicus pyruvate kinase, liver and RBC (Pklr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pklr ( NM_012624.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036756.3 Rattus norvegicus pyruvate kinase L/R (Pklr), mRNA.

Price & Availability↑

CloneID	ORa12225
Clone ID Related Accession (Same CDS sequence)	NM_012624.3
Accession Version	NM_012624.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate kinase PKLR
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC097039.8. On Apr 23, 2008 this sequence version replaced NM_012624.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M11709.1, BC161827.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906357, SAMN01906358 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGAAGGGC CAGCGGGATA CCTTCGACGT GCGAGTGTGG CTCAACTGAC CCAGGAGCTG 
GGCACTGCCT TCTTCCAGCA GCAGCAACTG CCCGCAGCTA TGGCGGACAC CTTCCTGGAA 
CACCTCTGCC TTCTGGATAT CGACTCACAG CCTGTGGCTG CTCGTAGCAC CAGCATCATT 
GCCACCATTG GGCCAGCATC CCGCTCTGTG GACCGCCTCA AGGAGATGAT CAAAGCAGGG 
ATGAACATTG CACGACTCAA CTTCTCCCAT GGCTCCCATG AGTACCATGC AGAATCCATC 
GCCAACATCC GGGAGGCAAC TGAGAGTTTT GCAACCTCCC CACTCAGCTA CAGACCTGTG 
GCCATCGCCC TGGACACCAA GGGACCTGAG ATACGAACCG GAGTCTTGCA GGGGGGTCCG 
GAGTCGGAGG TGGAAATTGT GAAGGGCTCA CAGGTGCTGG TGACGGTGGA CCCGAAGTTC 
CAGACAAGGG GCGATGCAAA GACAGTGTGG GTGGACTACC ACAATATCAC CCGGGTCGTT 
GCAGTGGGGG GCCGCATCTA CATTGACGAC GGGCTCATCT CCTTAGTGGT ACAGAAAATC 
GGCCCAGAGG GACTGGTGAC AGAAGTGGAG CACGGTGGTA TCTTGGGCAG CAGGAAGGGT 
GTGAACTTGC CAAACACTGA GGTGGACCTG CCCGGGCTGT CTGAGCAAGA CCTTTTGGAT 
CTGCGCTTCG GGGTGCAGCA TAATGTGGAC ATCATCTTTG CCTCCTTTGT GCGGAAAGCC 
AGTGACGTGT TAGCAGTCCG GGATGCCCTG GGGCCAGAAG GACAGAACAT CAAAATTATC 
AGCAAAATCG AGAACCATGA AGGCGTGAAG AAGTTTGATG AAATTCTAGA AGTGAGCGAT 
GGCATCATGG TGGCACGGGG TGACCTGGGC ATTGAGATCC CTGCGGAGAA GGTTTTCTTG 
GCTCAGAAGA TGATGATTGG ACGCTGCAAC CTGGCCGGCA AGCCTGTCGT TTGTGCCACA 
CAGATGCTGG AGAGCATGAT CACTAAGGCT CGACCAACTC GGGCGGAGAC AAGCGATGTG 
GCCAATGCCG TGCTGGATGG GGCTGACTGT ATCATGCTGT CCGGAGAGAC CGCCAAGGGC 
AGTTTTCCTG TGGAAGCTGT AATGATGCAA CATGCGATTG CGCGGGAGGC AGAGGCCGCT 
GTGTACCACC GCCAGTTGTT TGAGGAGCTA CGCCGGGCAG CGCCGCTGAG CCGTGACCCA 
ACTGAGGTCA CTGCGATTGG AGCCGTGGAG GCTTCCTTCA AGTGCTGTGC AGCAGCCATC 
ATCGTGCTGA CGAAGACTGG CCGTTCAGCC CAGCTTCTAT CTCAATACCG ACCTCGGGCG 
GCTGTCATTG CTGTGACTCG ATCTGCCCAG GCTGCCCGAC AGGTCCACCT GTCCCGAGGA 
GTCTTCCCCT TGCTCTACCG TGAGCCTCCA GAGGCCATCT GGGCAGATGA TGTGGATCGA 
AGGGTCCAAT TTGGCATTGA AAGTGGAAAG CTCCGTGGTT TCCTCCGTGT GGGTGATCTG 
GTGATTGTGG TGACAGGTTG GCGGCCTGGC TCTGGCTATA CCAACATCAT GCGGGTGCTG 
AGCGTATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036756.3
CDS	16..1647
Translation	MEGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS

Target ORF information:

RefSeq Version	NM_012624.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate kinase L/R (Pklr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012624.3

ORF Insert Sequence:

ATGGAAGGGC CAGCGGGATA CCTTCGACGT GCGAGTGTGG CTCAACTGAC CCAGGAGCTG 
GGCACTGCCT TCTTCCAGCA GCAGCAACTG CCCGCAGCTA TGGCGGACAC CTTCCTGGAA 
CACCTCTGCC TTCTGGATAT CGACTCACAG CCTGTGGCTG CTCGTAGCAC CAGCATCATT 
GCCACCATTG GGCCAGCATC CCGCTCTGTG GACCGCCTCA AGGAGATGAT CAAAGCAGGG 
ATGAACATTG CACGACTCAA CTTCTCCCAT GGCTCCCATG AGTACCATGC AGAATCCATC 
GCCAACATCC GGGAGGCAAC TGAGAGTTTT GCAACCTCCC CACTCAGCTA CAGACCTGTG 
GCCATCGCCC TGGACACCAA GGGACCTGAG ATACGAACCG GAGTCTTGCA GGGGGGTCCG 
GAGTCGGAGG TGGAAATTGT GAAGGGCTCA CAGGTGCTGG TGACGGTGGA CCCGAAGTTC 
CAGACAAGGG GCGATGCAAA GACAGTGTGG GTGGACTACC ACAATATCAC CCGGGTCGTT 
GCAGTGGGGG GCCGCATCTA CATTGACGAC GGGCTCATCT CCTTAGTGGT ACAGAAAATC 
GGCCCAGAGG GACTGGTGAC AGAAGTGGAG CACGGTGGTA TCTTGGGCAG CAGGAAGGGT 
GTGAACTTGC CAAACACTGA GGTGGACCTG CCCGGGCTGT CTGAGCAAGA CCTTTTGGAT 
CTGCGCTTCG GGGTGCAGCA TAATGTGGAC ATCATCTTTG CCTCCTTTGT GCGGAAAGCC 
AGTGACGTGT TAGCAGTCCG GGATGCCCTG GGGCCAGAAG GACAGAACAT CAAAATTATC 
AGCAAAATCG AGAACCATGA AGGCGTGAAG AAGTTTGATG AAATTCTAGA AGTGAGCGAT 
GGCATCATGG TGGCACGGGG TGACCTGGGC ATTGAGATCC CTGCGGAGAA GGTTTTCTTG 
GCTCAGAAGA TGATGATTGG ACGCTGCAAC CTGGCCGGCA AGCCTGTCGT TTGTGCCACA 
CAGATGCTGG AGAGCATGAT CACTAAGGCT CGACCAACTC GGGCGGAGAC AAGCGATGTG 
GCCAATGCCG TGCTGGATGG GGCTGACTGT ATCATGCTGT CCGGAGAGAC CGCCAAGGGC 
AGTTTTCCTG TGGAAGCTGT AATGATGCAA CATGCGATTG CGCGGGAGGC AGAGGCCGCT 
GTGTACCACC GCCAGTTGTT TGAGGAGCTA CGCCGGGCAG CGCCGCTGAG CCGTGACCCA 
ACTGAGGTCA CTGCGATTGG AGCCGTGGAG GCTTCCTTCA AGTGCTGTGC AGCAGCCATC 
ATCGTGCTGA CGAAGACTGG CCGTTCAGCC CAGCTTCTAT CTCAATACCG ACCTCGGGCG 
GCTGTCATTG CTGTGACTCG ATCTGCCCAG GCTGCCCGAC AGGTCCACCT GTCCCGAGGA 
GTCTTCCCCT TGCTCTACCG TGAGCCTCCA GAGGCCATCT GGGCAGATGA TGTGGATCGA 
AGGGTCCAAT TTGGCATTGA AAGTGGAAAG CTCCGTGGTT TCCTCCGTGT GGGTGATCTG 
GTGATTGTGG TGACAGGTTG GCGGCCTGGC TCTGGCTATA CCAACATCAT GCGGGTGCTG 
AGCGTATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pklr ( XM_006232593.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232655.1 Rattus norvegicus pyruvate kinase, liver and RBC (Pklr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24843
Clone ID Related Accession (Same CDS sequence)	XM_006232593.3
Accession Version	XM_006232593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate kinase PKLR isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGTCC AGGAGAACAC TCTACCCCAG CAGCTCTGGC CGTGGATCTT TAGGTCCCAA 
AAAGACTTGG CAAAGTCTGC CTTAAGTGGG GCTCCCGGAG GGCCAGCGGG ATACCTTCGA 
CGTGCGAGTG TGGCTCAACT GACCCAGGAG CTGGGCACTG CCTTCTTCCA GCAGCAGCAA 
CTGCCCGCAG CTATGGCGGA CACCTTCCTG GAACACCTCT GCCTTCTGGA TATCGACTCA 
CAGCCTGTGG CTGCTCGTAG CACCAGCATC ATTGCCACCA TTGGGCCAGC ATCCCGCTCT 
GTGGACCGCC TCAAGGAGAT GATCAAAGCA GGGATGAACA TTGCACGACT CAACTTCTCC 
CATGGCTCCC ATGAGTACCA TGCAGAATCC ATCGCCAACA TCCGGGAGGC AACTGAGAGT 
TTTGCAACCT CCCCACTCAG CTACAGACCT GTGGCCATCG CCCTGGACAC CAAGGGACCT 
GAGATACGAA CCGGAGTCTT GCAGGGGGGT CCGGAGTCGG AGGTGGAAAT TGTGAAGGGC 
TCACAGGTGC TGGTGACGGT GGACCCGAAG TTCCAGACAA GGGGCGATGC AAAGACAGTG 
TGGGTGGACT ACCACAATAT CACCCGGGTC GTTGCAGTGG GGGGCCGCAT CTACATTGAC 
GACGGGCTCA TCTCCTTAGT GGTACAGAAA ATCGGCCCAG AGGGACTGGT GACAGAAGTG 
GAGCACGGTG GTATCTTGGG CAGCAGGAAG GGTGTGAACT TGCCAAACAC TGAGGTGGAC 
CTGCCCGGGC TGTCTGAGCA AGACCTTTTG GATCTGCGCT TCGGGGTGCA GCATAATGTG 
GACATCATCT TTGCCTCCTT TGTGCGGAAA GCCAGTGACG TGTTAGCAGT CCGGGATGCC 
CTGGGGCCAG AAGGACAGAA CATCAAAATT ATCAGCAAAA TCGAGAACCA TGAAGGCGTG 
AAGAAGTTTG ATGAAATTCT AGAAGTGAGC GATGGCATCA TGGTGGCACG GGGTGACCTG 
GGCATTGAGA TCCCTGCGGA GAAGGTTTTC TTGGCTCAGA AGATGATGAT TGGACGCTGC 
AACCTGGCCG GCAAGCCTGT CGTTTGTGCC ACACAGATGC TGGAGAGCAT GATCACTAAG 
GCTCGACCAA CTCGGGCGGA GACAAGCGAT GTGGCCAATG CCGTGCTGGA TGGGGCTGAC 
TGTATCATGC TGTCCGGAGA GACCGCCAAG GGCAGTTTTC CTGTGGAAGC TGTAATGATG 
CAACATGCGA TTGCGCGGGA GGCAGAGGCC GCTGTGTACC ACCGCCAGTT GTTTGAGGAG 
CTACGCCGGG CAGCGCCGCT GAGCCGTGAC CCAACTGAGG TCACTGCGAT TGGAGCCGTG 
GAGGCTTCCT TCAAGTGCTG TGCAGCAGCC ATCATCGTGC TGACGAAGAC TGGCCGTTCA 
GCCCAGCTTC TATCTCAATA CCGACCTCGG GCGGCTGTCA TTGCTGTGAC TCGATCTGCC 
CAGGCTGCCC GACAGGTCCA CCTGTCCCGA GGAGTCTTCC CCTTGCTCTA CCGTGAGCCT 
CCAGAGGCCA TCTGGGCAGA TGATGTGGAT CGAAGGGTCC AATTTGGCAT TGAAAGTGGA 
AAGCTCCGTG GTTTCCTCCG TGTGGGTGAT CTGGTGATTG TGGTGACAGG TTGGCGGCCT 
GGCTCTGGCT ATACCAACAT CATGCGGGTG CTGAGCGTAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232655.1
CDS	53..1777
Translation	MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS

Target ORF information:

RefSeq Version	XM_006232593.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate kinase, liver and RBC (Pklr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232593.3

ORF Insert Sequence:

ATGTCTGTCC AGGAGAACAC TCTACCCCAG CAGCTCTGGC CGTGGATCTT TAGGTCCCAA 
AAAGACTTGG CAAAGTCTGC CTTAAGTGGG GCTCCCGGAG GGCCAGCGGG ATACCTTCGA 
CGTGCGAGTG TGGCTCAACT GACCCAGGAG CTGGGCACTG CCTTCTTCCA GCAGCAGCAA 
CTGCCCGCAG CTATGGCGGA CACCTTCCTG GAACACCTCT GCCTTCTGGA TATCGACTCA 
CAGCCTGTGG CTGCTCGTAG CACCAGCATC ATTGCCACCA TTGGGCCAGC ATCCCGCTCT 
GTGGACCGCC TCAAGGAGAT GATCAAAGCA GGGATGAACA TTGCACGACT CAACTTCTCC 
CATGGCTCCC ATGAGTACCA TGCAGAATCC ATCGCCAACA TCCGGGAGGC AACTGAGAGT 
TTTGCAACCT CCCCACTCAG CTACAGACCT GTGGCCATCG CCCTGGACAC CAAGGGACCT 
GAGATACGAA CCGGAGTCTT GCAGGGGGGT CCGGAGTCGG AGGTGGAAAT TGTGAAGGGC 
TCACAGGTGC TGGTGACGGT GGACCCGAAG TTCCAGACAA GGGGCGATGC AAAGACAGTG 
TGGGTGGACT ACCACAATAT CACCCGGGTC GTTGCAGTGG GGGGCCGCAT CTACATTGAC 
GACGGGCTCA TCTCCTTAGT GGTACAGAAA ATCGGCCCAG AGGGACTGGT GACAGAAGTG 
GAGCACGGTG GTATCTTGGG CAGCAGGAAG GGTGTGAACT TGCCAAACAC TGAGGTGGAC 
CTGCCCGGGC TGTCTGAGCA AGACCTTTTG GATCTGCGCT TCGGGGTGCA GCATAATGTG 
GACATCATCT TTGCCTCCTT TGTGCGGAAA GCCAGTGACG TGTTAGCAGT CCGGGATGCC 
CTGGGGCCAG AAGGACAGAA CATCAAAATT ATCAGCAAAA TCGAGAACCA TGAAGGCGTG 
AAGAAGTTTG ATGAAATTCT AGAAGTGAGC GATGGCATCA TGGTGGCACG GGGTGACCTG 
GGCATTGAGA TCCCTGCGGA GAAGGTTTTC TTGGCTCAGA AGATGATGAT TGGACGCTGC 
AACCTGGCCG GCAAGCCTGT CGTTTGTGCC ACACAGATGC TGGAGAGCAT GATCACTAAG 
GCTCGACCAA CTCGGGCGGA GACAAGCGAT GTGGCCAATG CCGTGCTGGA TGGGGCTGAC 
TGTATCATGC TGTCCGGAGA GACCGCCAAG GGCAGTTTTC CTGTGGAAGC TGTAATGATG 
CAACATGCGA TTGCGCGGGA GGCAGAGGCC GCTGTGTACC ACCGCCAGTT GTTTGAGGAG 
CTACGCCGGG CAGCGCCGCT GAGCCGTGAC CCAACTGAGG TCACTGCGAT TGGAGCCGTG 
GAGGCTTCCT TCAAGTGCTG TGCAGCAGCC ATCATCGTGC TGACGAAGAC TGGCCGTTCA 
GCCCAGCTTC TATCTCAATA CCGACCTCGG GCGGCTGTCA TTGCTGTGAC TCGATCTGCC 
CAGGCTGCCC GACAGGTCCA CCTGTCCCGA GGAGTCTTCC CCTTGCTCTA CCGTGAGCCT 
CCAGAGGCCA TCTGGGCAGA TGATGTGGAT CGAAGGGTCC AATTTGGCAT TGAAAGTGGA 
AAGCTCCGTG GTTTCCTCCG TGTGGGTGAT CTGGTGATTG TGGTGACAGG TTGGCGGCCT 
GGCTCTGGCT ATACCAACAT CATGCGGGTG CTGAGCGTAT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pklr ( XM_017590631.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446120.1 Rattus norvegicus pyruvate kinase, liver and RBC (Pklr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa59626
Clone ID Related Accession (Same CDS sequence)	XM_017590631.1
Accession Version	XM_017590631.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1533bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pyruvate kinase PKLR isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACA CCTTCCTGGA ACACCTCTGC CTTCTGGATA TCGACTCACA GCCTGTGGCT 
GCTCGTAGCA CCAGCATCAT TGCCACCATT GGGCCAGCAT CCCGCTCTGT GGACCGCCTC 
AAGGAGATGA TCAAAGCAGG GATGAACATT GCACGACTCA ACTTCTCCCA TGGCTCCCAT 
GAGTACCATG CAGAATCCAT CGCCAACATC CGGGAGGCAA CTGAGAGTTT TGCAACCTCC 
CCACTCAGCT ACAGACCTGT GGCCATCGCC CTGGACACCA AGGGACCTGA GATACGAACC 
GGAGTCTTGC AGGGGGGTCC GGAGTCGGAG GTGGAAATTG TGAAGGGCTC ACAGGTGCTG 
GTGACGGTGG ACCCGAAGTT CCAGACAAGG GGCGATGCAA AGACAGTGTG GGTGGACTAC 
CACAATATCA CCCGGGTCGT TGCAGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC 
TCCTTAGTGG TACAGAAAAT CGGCCCAGAG GGACTGGTGA CAGAAGTGGA GCACGGTGGT 
ATCTTGGGCA GCAGGAAGGG TGTGAACTTG CCAAACACTG AGGTGGACCT GCCCGGGCTG 
TCTGAGCAAG ACCTTTTGGA TCTGCGCTTC GGGGTGCAGC ATAATGTGGA CATCATCTTT 
GCCTCCTTTG TGCGGAAAGC CAGTGACGTG TTAGCAGTCC GGGATGCCCT GGGGCCAGAA 
GGACAGAACA TCAAAATTAT CAGCAAAATC GAGAACCATG AAGGCGTGAA GAAGTTTGAT 
GAAATTCTAG AAGTGAGCGA TGGCATCATG GTGGCACGGG GTGACCTGGG CATTGAGATC 
CCTGCGGAGA AGGTTTTCTT GGCTCAGAAG ATGATGATTG GACGCTGCAA CCTGGCCGGC 
AAGCCTGTCG TTTGTGCCAC ACAGATGCTG GAGAGCATGA TCACTAAGGC TCGACCAACT 
CGGGCGGAGA CAAGCGATGT GGCCAATGCC GTGCTGGATG GGGCTGACTG TATCATGCTG 
TCCGGAGAGA CCGCCAAGGG CAGTTTTCCT GTGGAAGCTG TAATGATGCA ACATGCGATT 
GCGCGGGAGG CAGAGGCCGC TGTGTACCAC CGCCAGTTGT TTGAGGAGCT ACGCCGGGCA 
GCGCCGCTGA GCCGTGACCC AACTGAGGTC ACTGCGATTG GAGCCGTGGA GGCTTCCTTC 
AAGTGCTGTG CAGCAGCCAT CATCGTGCTG ACGAAGACTG GCCGTTCAGC CCAGCTTCTA 
TCTCAATACC GACCTCGGGC GGCTGTCATT GCTGTGACTC GATCTGCCCA GGCTGCCCGA 
CAGGTCCACC TGTCCCGAGG AGTCTTCCCC TTGCTCTACC GTGAGCCTCC AGAGGCCATC 
TGGGCAGATG ATGTGGATCG AAGGGTCCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGT 
TTCCTCCGTG TGGGTGATCT GGTGATTGTG GTGACAGGTT GGCGGCCTGG CTCTGGCTAT 
ACCAACATCA TGCGGGTGCT GAGCGTATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446120.1
CDS	259..1791
Translation	MADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS

Target ORF information:

RefSeq Version	XM_017590631.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus pyruvate kinase, liver and RBC (Pklr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590631.1

ORF Insert Sequence:

ATGGCGGACA CCTTCCTGGA ACACCTCTGC CTTCTGGATA TCGACTCACA GCCTGTGGCT 
GCTCGTAGCA CCAGCATCAT TGCCACCATT GGGCCAGCAT CCCGCTCTGT GGACCGCCTC 
AAGGAGATGA TCAAAGCAGG GATGAACATT GCACGACTCA ACTTCTCCCA TGGCTCCCAT 
GAGTACCATG CAGAATCCAT CGCCAACATC CGGGAGGCAA CTGAGAGTTT TGCAACCTCC 
CCACTCAGCT ACAGACCTGT GGCCATCGCC CTGGACACCA AGGGACCTGA GATACGAACC 
GGAGTCTTGC AGGGGGGTCC GGAGTCGGAG GTGGAAATTG TGAAGGGCTC ACAGGTGCTG 
GTGACGGTGG ACCCGAAGTT CCAGACAAGG GGCGATGCAA AGACAGTGTG GGTGGACTAC 
CACAATATCA CCCGGGTCGT TGCAGTGGGG GGCCGCATCT ACATTGACGA CGGGCTCATC 
TCCTTAGTGG TACAGAAAAT CGGCCCAGAG GGACTGGTGA CAGAAGTGGA GCACGGTGGT 
ATCTTGGGCA GCAGGAAGGG TGTGAACTTG CCAAACACTG AGGTGGACCT GCCCGGGCTG 
TCTGAGCAAG ACCTTTTGGA TCTGCGCTTC GGGGTGCAGC ATAATGTGGA CATCATCTTT 
GCCTCCTTTG TGCGGAAAGC CAGTGACGTG TTAGCAGTCC GGGATGCCCT GGGGCCAGAA 
GGACAGAACA TCAAAATTAT CAGCAAAATC GAGAACCATG AAGGCGTGAA GAAGTTTGAT 
GAAATTCTAG AAGTGAGCGA TGGCATCATG GTGGCACGGG GTGACCTGGG CATTGAGATC 
CCTGCGGAGA AGGTTTTCTT GGCTCAGAAG ATGATGATTG GACGCTGCAA CCTGGCCGGC 
AAGCCTGTCG TTTGTGCCAC ACAGATGCTG GAGAGCATGA TCACTAAGGC TCGACCAACT 
CGGGCGGAGA CAAGCGATGT GGCCAATGCC GTGCTGGATG GGGCTGACTG TATCATGCTG 
TCCGGAGAGA CCGCCAAGGG CAGTTTTCCT GTGGAAGCTG TAATGATGCA ACATGCGATT 
GCGCGGGAGG CAGAGGCCGC TGTGTACCAC CGCCAGTTGT TTGAGGAGCT ACGCCGGGCA 
GCGCCGCTGA GCCGTGACCC AACTGAGGTC ACTGCGATTG GAGCCGTGGA GGCTTCCTTC 
AAGTGCTGTG CAGCAGCCAT CATCGTGCTG ACGAAGACTG GCCGTTCAGC CCAGCTTCTA 
TCTCAATACC GACCTCGGGC GGCTGTCATT GCTGTGACTC GATCTGCCCA GGCTGCCCGA 
CAGGTCCACC TGTCCCGAGG AGTCTTCCCC TTGCTCTACC GTGAGCCTCC AGAGGCCATC 
TGGGCAGATG ATGTGGATCG AAGGGTCCAA TTTGGCATTG AAAGTGGAAA GCTCCGTGGT 
TTCCTCCGTG TGGGTGATCT GGTGATTGTG GTGACAGGTT GGCGGCCTGG CTCTGGCTAT 
ACCAACATCA TGCGGGTGCT GAGCGTATCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Red blood cell PK deficiency: An update of PK-LR gene mutation database.
Blood cells, molecules & diseases57100-9(2016 Mar)
Canu G,De Bonis M,Minucci A,Capoluongo E

Probing L-pyruvate kinase regulatory phosphorylation site by mutagenesis.
The protein journal31(7)592-7(2012 Oct)
Faustova I,Loog M,J?rv J

Hypermethylation of hepatic glucokinase and L-type pyruvate kinase promoters in high-fat diet-induced obese rats.
Endocrinology152(4)1284-9(2011 Apr)
Jiang M,Zhang Y,Liu M,Lan MS,Fei J,Fan W,Gao X,Lu D

Malaria: looking for selection signatures in the human PKLR gene region.
British journal of haematology149(5)775-84(2010 Jun)
Machado P,Pereira R,Rocha AM,Manco L,Fernandes N,Miranda J,Ribeiro L,do Ros?rio VE,Amorim A,Gusm?o L,Arez AP

Rescue of pyruvate kinase deficiency in mice by gene therapy using the human isoenzyme.
Molecular therapy : the journal of the American Society of Gene Therapy17(12)2000-9(2009 Dec)
Meza NW,Alonso-Ferrero ME,Navarro S,Quintana-Bustamante O,Valeri A,Garcia-Gomez M,Bueren JA,Bautista JM,Segovia JC

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Pklr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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