Pfkfb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pfkfb1
Entrez Gene ID	24638
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Synonyms	PFRX,Pfkfb01
General protein information	Preferred Names 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 Names 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 6-PF2-K/Fru-2,6-P2ase 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 6PF-2-K/Fru-2,6-P2ASE liver isozyme 6PF-2-K/Fru-2,6-P2ase 1 PFK/FBPase 1 PFK2/FBPase2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13
Summary	bifunctional enzyme; catalyzes synthesis and degradation of fructose 2,6-bisphosphate, a ubiquitous stimulator of glycolysis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008773123.1	XP_008771345.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
XM_006256790.3	XP_006256852.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X2
NM_001271063.1	NP_001257992.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
NM_012621.4	NP_036753.4	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
XM_006256791.3	XP_006256853.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3
XM_008773125.1	XP_008771347.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X4
XM_008773124.2	XP_008771346.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
NM_001271064.1	NP_001257993.1	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100488795	XP_004919688.1
Bos taurus (cattle)	PFKFB1	NP_776997.3
Rattus norvegicus (Norway rat)	Pfkfb1	NP_036753.4
Danio rerio (zebrafish)	LOC100330415	XP_002666487.2
	PFKFB1	XP_001091907.2
Canis lupus familiaris (dog)	PFKFB1	XP_005641453.1
Mus musculus (house mouse)	Pfkfb1	NP_032850.1
Homo sapiens (human)	PFKFB1	NP_002616.2
Gallus gallus (chicken)	PFKFB4	NP_001025755.1

Gene Ontology
Bibliography

Related articles in PubMed

Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes.
Gibb AA, Lorkiewicz PK, Zheng YT, Zhang X, Bhatnagar A, Jones SP, Hill BG
The Biochemical journal474(16)2785-2801(2017 08)

Identification of a novel Pfkfb1 mRNA variant in rat fetal liver.
Cosin-Roger J, Vernia S, Alvarez MS, Cucarella C, Bosc? L, Martin-Sanz P, Fern?ndez-Alvarez AJ, Casado M
Biochemical and biophysical research communications431(1)36-40(2013 Feb)

The mechanism of calcium-induced inhibition of muscle fructose 1,6-bisphosphatase and destabilization of glyconeogenic complex.
Rakus D, Gizak A, Kasprzak AA, Zarzycki M, Maciaszczyk-Dziubinska E, Dzugaj A
PloS one8(10)e76669(2013)

Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation.
Langer S, Okar DA, Schultz J, Lenzen S, Baltrusch S
FEBS letters586(10)1419-25(2012 May)

The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate.
Manes NP, El-Maghrabi MR
Archives of biochemistry and biophysics438(2)125-36(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that a major portion of intracellular glucose in cardiac myocytes is apportioned for ancillary biosynthetic reactions; PFKFB1 coordinates activities of pentose phosphate, hexosamine biosynthetic, and fatty acid/glycerolipid synthesis pathways by directly modulating glycolytic intermediate entry into auxiliary glucose metabolism pathways and by indirectly regulating mitochondrial cataplerosis.
Title: Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes.

a new mRNA variant which are transcribed from a new Pfkfb1 gene promoter in fetal rat liver was characterized.
Title: Identification of a novel Pfkfb1 mRNA variant in rat fetal liver.

Ca(2+) affects conformation of the catalytic loop 52-72 of muscle FBPase and inhibits its activity by competing with activatory divalent cations, e.g. Mg(2+) and Zn(2+).
Title: The mechanism of calcium-induced inhibition of muscle fructose 1,6-bisphosphatase and destabilization of glyconeogenic complex.

flexible kinase interaction mode exists, suggesting dimer conformation mediated coupling of hormonal and posttranslational enzyme regulation to the metabolic response in liver
Title: Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation.

Results describe structural differences between two isozymes of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase from liver and testis.
Title: Tissue-specific structure/function differentiation of the liver isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase.

The following Pfkfb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pfkfb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00480	NM_012621.4 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa10135	NM_001271063.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa10140	NM_001271064.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa10135	XM_008773123.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa10135	XM_008773124.2 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa10140	XM_008773125.1 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa39644	XM_006256790.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa39645	XM_006256791.3 Latest version!	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pfkfb1 ( NM_012621.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036753.4 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa00480
Clone ID Related Accession (Same CDS sequence)	NM_012621.4
Accession Version	NM_012621.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from Y00702.1. On Oct 14, 2007 this sequence version replaced NM_012621.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y00702.1, J04197.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTCGAG AGATGGGAGA ACTCACTCAA ACCAGGTTAC AGAAGATCTG GATTCCACAC 
AGCAGCAGTA GCAGCGTGCT GCAACGGCGA AGGGGCTCCT CCATACCACA GTTCACTAAT 
TCTCCCACGA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA CATCTCTACG 
AAGCTCACAC GCTATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGTCAG 
TATCGACGAG AGGCAGTGAG TTACAGGAAC TATGAATTCT TTCGCCCAGA CAACACAGAG 
GCCCAGCTTA TCAGGAAGCA GTGTGCTCTA GCAGCCCTAA AGGATGTCCA TAAGTATCTC 
AGCCGCGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGA 
TCGTTGATTC TACAGTTTGC TAAGGAACAT GGTTATAAGG TCTTCTTTAT TGAGTCTATT 
TGTAATGACC CCGAAATCAT TGCAGAAAAC ATCAAGCAAG TGAAACTTGG TAGTCCTGAT 
TACATAGACT GTGACCAAGA AAAGGTTTTG GAAGACTTTC TAAAGAGAAT AGAGTGCTAT 
GAGATCAACT ACCAACCTTT GGATGAGGAA TTGGACAGCC ACCTGTCCTA CATCAAGATC 
TTCGACGTGG GCACACGCTA CATGGTAAAT CGAGTGCAGG ACCACGTTCA GAGCCGTACA 
GCCTACTACC TCATGAACAT CCATGTCACA CCTCGATCTA TCTACCTATG CCGCCATGGT 
GAGAGTGAAC TCAACCTTAG AGGCCGCATT GGAGGTGACT CTGGCCTCTC AGCTCGGGGC 
AAGCAGTATG CCTATGCACT AGCCAACTTC ATCCGGTCTC AAGGCATCAG CTCCCTGAAA 
GTATGGACTA GCCACATGAA GAGGACCATT CAGACCGCTG AAGCCCTAGG TGTCCCCTAT 
GAACAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAAGAGAT GACCTATGAA 
GAAATTCAGG AACACTACCC TGAGGAATTT GCACTACGGG ACCAGGATAA ATATCGTTAC 
CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTTGAACC AGTTATAATG 
GAGCTAGAAC GGCAAGAAAA TGTACTGGTG ATCTGTCACC AGGCTGTCAT GCGGTGCCTC 
CTGGCATACT TCCTGGATAA AAGTTCAGAT GAGCTGCCCT ATCTCAAGTG TCCTCTGCAT 
ACTGTGCTCA AACTCACACC TGTGGCTTAT GGCTGCAGAG TGGAGTCCAT CTACCTGAAT 
GTGGAGGCTG TGAACACACA CCGGGACAAG CCTGAGAATG TGGACATCAC CCGTGAAGCT 
GAGGAAGCCT TGGACACTGT ACCTGCCCAT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036753.4
CDS	173..1588
Translation	MSREMGELTQTRLQKIWIPHSSSSSVLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_012621.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012621.4

ORF Insert Sequence:

ATGTCTCGAG AGATGGGAGA ACTCACTCAA ACCAGGTTAC AGAAGATCTG GATTCCACAC 
AGCAGCAGTA GCAGCGTGCT GCAACGGCGA AGGGGCTCCT CCATACCACA GTTCACTAAT 
TCTCCCACGA TGGTGATCAT GGTGGGTTTA CCAGCTCGAG GCAAGACCTA CATCTCTACG 
AAGCTCACAC GCTATCTCAA CTGGATAGGA ACACCAACTA AAGTGTTTAA TTTAGGTCAG 
TATCGACGAG AGGCAGTGAG TTACAGGAAC TATGAATTCT TTCGCCCAGA CAACACAGAG 
GCCCAGCTTA TCAGGAAGCA GTGTGCTCTA GCAGCCCTAA AGGATGTCCA TAAGTATCTC 
AGCCGCGAGG AAGGTCATGT TGCGGTTTTT GATGCCACCA ACACTACCAG AGAACGACGA 
TCGTTGATTC TACAGTTTGC TAAGGAACAT GGTTATAAGG TCTTCTTTAT TGAGTCTATT 
TGTAATGACC CCGAAATCAT TGCAGAAAAC ATCAAGCAAG TGAAACTTGG TAGTCCTGAT 
TACATAGACT GTGACCAAGA AAAGGTTTTG GAAGACTTTC TAAAGAGAAT AGAGTGCTAT 
GAGATCAACT ACCAACCTTT GGATGAGGAA TTGGACAGCC ACCTGTCCTA CATCAAGATC 
TTCGACGTGG GCACACGCTA CATGGTAAAT CGAGTGCAGG ACCACGTTCA GAGCCGTACA 
GCCTACTACC TCATGAACAT CCATGTCACA CCTCGATCTA TCTACCTATG CCGCCATGGT 
GAGAGTGAAC TCAACCTTAG AGGCCGCATT GGAGGTGACT CTGGCCTCTC AGCTCGGGGC 
AAGCAGTATG CCTATGCACT AGCCAACTTC ATCCGGTCTC AAGGCATCAG CTCCCTGAAA 
GTATGGACTA GCCACATGAA GAGGACCATT CAGACCGCTG AAGCCCTAGG TGTCCCCTAT 
GAACAGTGGA AGGCCCTGAA TGAGATTGAT GCGGGTGTCT GTGAAGAGAT GACCTATGAA 
GAAATTCAGG AACACTACCC TGAGGAATTT GCACTACGGG ACCAGGATAA ATATCGTTAC 
CGCTATCCCA AGGGAGAGTC CTATGAGGAT CTGGTTCAGC GTCTTGAACC AGTTATAATG 
GAGCTAGAAC GGCAAGAAAA TGTACTGGTG ATCTGTCACC AGGCTGTCAT GCGGTGCCTC 
CTGGCATACT TCCTGGATAA AAGTTCAGAT GAGCTGCCCT ATCTCAAGTG TCCTCTGCAT 
ACTGTGCTCA AACTCACACC TGTGGCTTAT GGCTGCAGAG TGGAGTCCAT CTACCTGAAT 
GTGGAGGCTG TGAACACACA CCGGGACAAG CCTGAGAATG TGGACATCAC CCGTGAAGCT 
GAGGAAGCCT TGGACACTGT ACCTGCCCAT TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb1 ( NM_001271063.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257992.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa10135
Clone ID Related Accession (Same CDS sequence)	XM_008773123.1 , NM_001271063.1 , XM_008773124.2
Accession Version	NM_001271063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07037521.1 and AABR07037520.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X15579.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GCCTCCATAC CACAGTTCAC TAATTCTCCC 
ACGATGGTGA TCATGGTGGG TTTACCAGCT CGAGGCAAGA CCTACATCTC TACGAAGCTC 
ACACGCTATC TCAACTGGAT AGGAACACCA ACTAAAGTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257992.1
CDS	56..1465
Translation	MPPTGPALGVCKCKRAIGKLAMEEKASKRTASIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_001271063.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271063.1

ORF Insert Sequence:

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GCCTCCATAC CACAGTTCAC TAATTCTCCC 
ACGATGGTGA TCATGGTGGG TTTACCAGCT CGAGGCAAGA CCTACATCTC TACGAAGCTC 
ACACGCTATC TCAACTGGAT AGGAACACCA ACTAAAGTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb1 ( NM_001271064.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257993.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa10140
Clone ID Related Accession (Same CDS sequence)	XM_008773125.1 , NM_001271064.1
Accession Version	NM_001271064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07037521.1 and AABR07037520.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ422136.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GTGTTTAATT TAGGTCAGTA TCGACGAGAG 
GCAGTGAGTT ACAGGAACTA TGAATTCTTT CGCCCAGACA ACACAGAGGC CCAGCTTATC 
AGGAAGCAGT GTGCTCTAGC AGCCCTAAAG GATGTCCATA AGTATCTCAG CCGCGAGGAA 
GGTCATGTTG CGGTTTTTGA TGCCACCAAC ACTACCAGAG AACGACGATC GTTGATTCTA 
CAGTTTGCTA AGGAACATGG TTATAAGGTC TTCTTTATTG AGTCTATTTG TAATGACCCC 
GAAATCATTG CAGAAAACAT CAAGCAAGTG AAACTTGGTA GTCCTGATTA CATAGACTGT 
GACCAAGAAA AGGTTTTGGA AGACTTTCTA AAGAGAATAG AGTGCTATGA GATCAACTAC 
CAACCTTTGG ATGAGGAATT GGACAGCCAC CTGTCCTACA TCAAGATCTT CGACGTGGGC 
ACACGCTACA TGGTAAATCG AGTGCAGGAC CACGTTCAGA GCCGTACAGC CTACTACCTC 
ATGAACATCC ATGTCACACC TCGATCTATC TACCTATGCC GCCATGGTGA GAGTGAACTC 
AACCTTAGAG GCCGCATTGG AGGTGACTCT GGCCTCTCAG CTCGGGGCAA GCAGTATGCC 
TATGCACTAG CCAACTTCAT CCGGTCTCAA GGCATCAGCT CCCTGAAAGT ATGGACTAGC 
CACATGAAGA GGACCATTCA GACCGCTGAA GCCCTAGGTG TCCCCTATGA ACAGTGGAAG 
GCCCTGAATG AGATTGATGC GGGTGTCTGT GAAGAGATGA CCTATGAAGA AATTCAGGAA 
CACTACCCTG AGGAATTTGC ACTACGGGAC CAGGATAAAT ATCGTTACCG CTATCCCAAG 
GGAGAGTCCT ATGAGGATCT GGTTCAGCGT CTTGAACCAG TTATAATGGA GCTAGAACGG 
CAAGAAAATG TACTGGTGAT CTGTCACCAG GCTGTCATGC GGTGCCTCCT GGCATACTTC 
CTGGATAAAA GTTCAGATGA GCTGCCCTAT CTCAAGTGTC CTCTGCATAC TGTGCTCAAA 
CTCACACCTG TGGCTTATGG CTGCAGAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTG 
AACACACACC GGGACAAGCC TGAGAATGTG GACATCACCC GTGAAGCTGA GGAAGCCTTG 
GACACTGTAC CTGCCCATTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257993.1
CDS	56..1339
Translation	MPPTGPALGVCKCKRAIGKLAMEEKASKRTVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	NM_001271064.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271064.1

ORF Insert Sequence:

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GTGTTTAATT TAGGTCAGTA TCGACGAGAG 
GCAGTGAGTT ACAGGAACTA TGAATTCTTT CGCCCAGACA ACACAGAGGC CCAGCTTATC 
AGGAAGCAGT GTGCTCTAGC AGCCCTAAAG GATGTCCATA AGTATCTCAG CCGCGAGGAA 
GGTCATGTTG CGGTTTTTGA TGCCACCAAC ACTACCAGAG AACGACGATC GTTGATTCTA 
CAGTTTGCTA AGGAACATGG TTATAAGGTC TTCTTTATTG AGTCTATTTG TAATGACCCC 
GAAATCATTG CAGAAAACAT CAAGCAAGTG AAACTTGGTA GTCCTGATTA CATAGACTGT 
GACCAAGAAA AGGTTTTGGA AGACTTTCTA AAGAGAATAG AGTGCTATGA GATCAACTAC 
CAACCTTTGG ATGAGGAATT GGACAGCCAC CTGTCCTACA TCAAGATCTT CGACGTGGGC 
ACACGCTACA TGGTAAATCG AGTGCAGGAC CACGTTCAGA GCCGTACAGC CTACTACCTC 
ATGAACATCC ATGTCACACC TCGATCTATC TACCTATGCC GCCATGGTGA GAGTGAACTC 
AACCTTAGAG GCCGCATTGG AGGTGACTCT GGCCTCTCAG CTCGGGGCAA GCAGTATGCC 
TATGCACTAG CCAACTTCAT CCGGTCTCAA GGCATCAGCT CCCTGAAAGT ATGGACTAGC 
CACATGAAGA GGACCATTCA GACCGCTGAA GCCCTAGGTG TCCCCTATGA ACAGTGGAAG 
GCCCTGAATG AGATTGATGC GGGTGTCTGT GAAGAGATGA CCTATGAAGA AATTCAGGAA 
CACTACCCTG AGGAATTTGC ACTACGGGAC CAGGATAAAT ATCGTTACCG CTATCCCAAG 
GGAGAGTCCT ATGAGGATCT GGTTCAGCGT CTTGAACCAG TTATAATGGA GCTAGAACGG 
CAAGAAAATG TACTGGTGAT CTGTCACCAG GCTGTCATGC GGTGCCTCCT GGCATACTTC 
CTGGATAAAA GTTCAGATGA GCTGCCCTAT CTCAAGTGTC CTCTGCATAC TGTGCTCAAA 
CTCACACCTG TGGCTTATGG CTGCAGAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTG 
AACACACACC GGGACAAGCC TGAGAATGTG GACATCACCC GTGAAGCTGA GGAAGCCTTG 
GACACTGTAC CTGCCCATTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb1 ( XM_008773123.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771345.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X1, mRNA.

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CloneID	ORa10135
Clone ID Related Accession (Same CDS sequence)	XM_008773123.1 , NM_001271063.1 , XM_008773124.2
Accession Version	XM_008773123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GCCTCCATAC CACAGTTCAC TAATTCTCCC 
ACGATGGTGA TCATGGTGGG TTTACCAGCT CGAGGCAAGA CCTACATCTC TACGAAGCTC 
ACACGCTATC TCAACTGGAT AGGAACACCA ACTAAAGTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771345.1
CDS	230..1639
Translation	MPPTGPALGVCKCKRAIGKLAMEEKASKRTASIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_008773123.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773123.1

ORF Insert Sequence:

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GCCTCCATAC CACAGTTCAC TAATTCTCCC 
ACGATGGTGA TCATGGTGGG TTTACCAGCT CGAGGCAAGA CCTACATCTC TACGAAGCTC 
ACACGCTATC TCAACTGGAT AGGAACACCA ACTAAAGTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb1 ( XM_008773124.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771346.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X2, mRNA.

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CloneID	ORa10135
Clone ID Related Accession (Same CDS sequence)	XM_008773123.1 , NM_001271063.1 , XM_008773124.2
Accession Version	XM_008773124.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008773124.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GCCTCCATAC CACAGTTCAC TAATTCTCCC 
ACGATGGTGA TCATGGTGGG TTTACCAGCT CGAGGCAAGA CCTACATCTC TACGAAGCTC 
ACACGCTATC TCAACTGGAT AGGAACACCA ACTAAAGTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771346.1
CDS	230..1639
Translation	MPPTGPALGVCKCKRAIGKLAMEEKASKRTASIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_008773124.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773124.2

ORF Insert Sequence:

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GCCTCCATAC CACAGTTCAC TAATTCTCCC 
ACGATGGTGA TCATGGTGGG TTTACCAGCT CGAGGCAAGA CCTACATCTC TACGAAGCTC 
ACACGCTATC TCAACTGGAT AGGAACACCA ACTAAAGTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb1 ( XM_008773125.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771347.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa10140
Clone ID Related Accession (Same CDS sequence)	XM_008773125.1 , NM_001271064.1
Accession Version	XM_008773125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GTGTTTAATT TAGGTCAGTA TCGACGAGAG 
GCAGTGAGTT ACAGGAACTA TGAATTCTTT CGCCCAGACA ACACAGAGGC CCAGCTTATC 
AGGAAGCAGT GTGCTCTAGC AGCCCTAAAG GATGTCCATA AGTATCTCAG CCGCGAGGAA 
GGTCATGTTG CGGTTTTTGA TGCCACCAAC ACTACCAGAG AACGACGATC GTTGATTCTA 
CAGTTTGCTA AGGAACATGG TTATAAGGTC TTCTTTATTG AGTCTATTTG TAATGACCCC 
GAAATCATTG CAGAAAACAT CAAGCAAGTG AAACTTGGTA GTCCTGATTA CATAGACTGT 
GACCAAGAAA AGGTTTTGGA AGACTTTCTA AAGAGAATAG AGTGCTATGA GATCAACTAC 
CAACCTTTGG ATGAGGAATT GGACAGCCAC CTGTCCTACA TCAAGATCTT CGACGTGGGC 
ACACGCTACA TGGTAAATCG AGTGCAGGAC CACGTTCAGA GCCGTACAGC CTACTACCTC 
ATGAACATCC ATGTCACACC TCGATCTATC TACCTATGCC GCCATGGTGA GAGTGAACTC 
AACCTTAGAG GCCGCATTGG AGGTGACTCT GGCCTCTCAG CTCGGGGCAA GCAGTATGCC 
TATGCACTAG CCAACTTCAT CCGGTCTCAA GGCATCAGCT CCCTGAAAGT ATGGACTAGC 
CACATGAAGA GGACCATTCA GACCGCTGAA GCCCTAGGTG TCCCCTATGA ACAGTGGAAG 
GCCCTGAATG AGATTGATGC GGGTGTCTGT GAAGAGATGA CCTATGAAGA AATTCAGGAA 
CACTACCCTG AGGAATTTGC ACTACGGGAC CAGGATAAAT ATCGTTACCG CTATCCCAAG 
GGAGAGTCCT ATGAGGATCT GGTTCAGCGT CTTGAACCAG TTATAATGGA GCTAGAACGG 
CAAGAAAATG TACTGGTGAT CTGTCACCAG GCTGTCATGC GGTGCCTCCT GGCATACTTC 
CTGGATAAAA GTTCAGATGA GCTGCCCTAT CTCAAGTGTC CTCTGCATAC TGTGCTCAAA 
CTCACACCTG TGGCTTATGG CTGCAGAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTG 
AACACACACC GGGACAAGCC TGAGAATGTG GACATCACCC GTGAAGCTGA GGAAGCCTTG 
GACACTGTAC CTGCCCATTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771347.1
CDS	230..1513
Translation	MPPTGPALGVCKCKRAIGKLAMEEKASKRTVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_008773125.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773125.1

ORF Insert Sequence:

ATGCCACCCA CCGGACCTGC TTTGGGGGTC TGTAAATGCA AGAGAGCCAT TGGAAAATTA 
GCGATGGAAG AAAAAGCCTC TAAGAGAACA GTGTTTAATT TAGGTCAGTA TCGACGAGAG 
GCAGTGAGTT ACAGGAACTA TGAATTCTTT CGCCCAGACA ACACAGAGGC CCAGCTTATC 
AGGAAGCAGT GTGCTCTAGC AGCCCTAAAG GATGTCCATA AGTATCTCAG CCGCGAGGAA 
GGTCATGTTG CGGTTTTTGA TGCCACCAAC ACTACCAGAG AACGACGATC GTTGATTCTA 
CAGTTTGCTA AGGAACATGG TTATAAGGTC TTCTTTATTG AGTCTATTTG TAATGACCCC 
GAAATCATTG CAGAAAACAT CAAGCAAGTG AAACTTGGTA GTCCTGATTA CATAGACTGT 
GACCAAGAAA AGGTTTTGGA AGACTTTCTA AAGAGAATAG AGTGCTATGA GATCAACTAC 
CAACCTTTGG ATGAGGAATT GGACAGCCAC CTGTCCTACA TCAAGATCTT CGACGTGGGC 
ACACGCTACA TGGTAAATCG AGTGCAGGAC CACGTTCAGA GCCGTACAGC CTACTACCTC 
ATGAACATCC ATGTCACACC TCGATCTATC TACCTATGCC GCCATGGTGA GAGTGAACTC 
AACCTTAGAG GCCGCATTGG AGGTGACTCT GGCCTCTCAG CTCGGGGCAA GCAGTATGCC 
TATGCACTAG CCAACTTCAT CCGGTCTCAA GGCATCAGCT CCCTGAAAGT ATGGACTAGC 
CACATGAAGA GGACCATTCA GACCGCTGAA GCCCTAGGTG TCCCCTATGA ACAGTGGAAG 
GCCCTGAATG AGATTGATGC GGGTGTCTGT GAAGAGATGA CCTATGAAGA AATTCAGGAA 
CACTACCCTG AGGAATTTGC ACTACGGGAC CAGGATAAAT ATCGTTACCG CTATCCCAAG 
GGAGAGTCCT ATGAGGATCT GGTTCAGCGT CTTGAACCAG TTATAATGGA GCTAGAACGG 
CAAGAAAATG TACTGGTGAT CTGTCACCAG GCTGTCATGC GGTGCCTCCT GGCATACTTC 
CTGGATAAAA GTTCAGATGA GCTGCCCTAT CTCAAGTGTC CTCTGCATAC TGTGCTCAAA 
CTCACACCTG TGGCTTATGG CTGCAGAGTG GAGTCCATCT ACCTGAATGT GGAGGCTGTG 
AACACACACC GGGACAAGCC TGAGAATGTG GACATCACCC GTGAAGCTGA GGAAGCCTTG 
GACACTGTAC CTGCCCATTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb1 ( XM_006256790.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256852.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X3, mRNA.

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CloneID	ORa39644
Clone ID Related Accession (Same CDS sequence)	XM_006256790.3
Accession Version	XM_006256790.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256790.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGAG AGATGGGAGA ACTCACTCAA ACCAGGTTAC AGAAGATCTG GATTCCACAC 
AGCAGCAGTA GCAGCGTGCT GCAACGGCGA AGGGGCTTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256852.1
CDS	3783..5072
Translation	MSREMGELTQTRLQKIWIPHSSSSSVLQRRRGLFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_006256790.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256790.3

ORF Insert Sequence:

ATGTCTCGAG AGATGGGAGA ACTCACTCAA ACCAGGTTAC AGAAGATCTG GATTCCACAC 
AGCAGCAGTA GCAGCGTGCT GCAACGGCGA AGGGGCTTGT TTAATTTAGG TCAGTATCGA 
CGAGAGGCAG TGAGTTACAG GAACTATGAA TTCTTTCGCC CAGACAACAC AGAGGCCCAG 
CTTATCAGGA AGCAGTGTGC TCTAGCAGCC CTAAAGGATG TCCATAAGTA TCTCAGCCGC 
GAGGAAGGTC ATGTTGCGGT TTTTGATGCC ACCAACACTA CCAGAGAACG ACGATCGTTG 
ATTCTACAGT TTGCTAAGGA ACATGGTTAT AAGGTCTTCT TTATTGAGTC TATTTGTAAT 
GACCCCGAAA TCATTGCAGA AAACATCAAG CAAGTGAAAC TTGGTAGTCC TGATTACATA 
GACTGTGACC AAGAAAAGGT TTTGGAAGAC TTTCTAAAGA GAATAGAGTG CTATGAGATC 
AACTACCAAC CTTTGGATGA GGAATTGGAC AGCCACCTGT CCTACATCAA GATCTTCGAC 
GTGGGCACAC GCTACATGGT AAATCGAGTG CAGGACCACG TTCAGAGCCG TACAGCCTAC 
TACCTCATGA ACATCCATGT CACACCTCGA TCTATCTACC TATGCCGCCA TGGTGAGAGT 
GAACTCAACC TTAGAGGCCG CATTGGAGGT GACTCTGGCC TCTCAGCTCG GGGCAAGCAG 
TATGCCTATG CACTAGCCAA CTTCATCCGG TCTCAAGGCA TCAGCTCCCT GAAAGTATGG 
ACTAGCCACA TGAAGAGGAC CATTCAGACC GCTGAAGCCC TAGGTGTCCC CTATGAACAG 
TGGAAGGCCC TGAATGAGAT TGATGCGGGT GTCTGTGAAG AGATGACCTA TGAAGAAATT 
CAGGAACACT ACCCTGAGGA ATTTGCACTA CGGGACCAGG ATAAATATCG TTACCGCTAT 
CCCAAGGGAG AGTCCTATGA GGATCTGGTT CAGCGTCTTG AACCAGTTAT AATGGAGCTA 
GAACGGCAAG AAAATGTACT GGTGATCTGT CACCAGGCTG TCATGCGGTG CCTCCTGGCA 
TACTTCCTGG ATAAAAGTTC AGATGAGCTG CCCTATCTCA AGTGTCCTCT GCATACTGTG 
CTCAAACTCA CACCTGTGGC TTATGGCTGC AGAGTGGAGT CCATCTACCT GAATGTGGAG 
GCTGTGAACA CACACCGGGA CAAGCCTGAG AATGTGGACA TCACCCGTGA AGCTGAGGAA 
GCCTTGGACA CTGTACCTGC CCATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pfkfb1 ( XM_006256791.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256853.1 Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa39645
Clone ID Related Accession (Same CDS sequence)	XM_006256791.3
Accession Version	XM_006256791.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256791.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ACATCTCTAC GAAGCTCACA 
CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGTCA GTATCGACGA 
GAGGCAGTGA GTTACAGGAA CTATGAATTC TTTCGCCCAG ACAACACAGA GGCCCAGCTT 
ATCAGGAAGC AGTGTGCTCT AGCAGCCCTA AAGGATGTCC ATAAGTATCT CAGCCGCGAG 
GAAGGTCATG TTGCGGTTTT TGATGCCACC AACACTACCA GAGAACGACG ATCGTTGATT 
CTACAGTTTG CTAAGGAACA TGGTTATAAG GTCTTCTTTA TTGAGTCTAT TTGTAATGAC 
CCCGAAATCA TTGCAGAAAA CATCAAGCAA GTGAAACTTG GTAGTCCTGA TTACATAGAC 
TGTGACCAAG AAAAGGTTTT GGAAGACTTT CTAAAGAGAA TAGAGTGCTA TGAGATCAAC 
TACCAACCTT TGGATGAGGA ATTGGACAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG 
GGCACACGCT ACATGGTAAA TCGAGTGCAG GACCACGTTC AGAGCCGTAC AGCCTACTAC 
CTCATGAACA TCCATGTCAC ACCTCGATCT ATCTACCTAT GCCGCCATGG TGAGAGTGAA 
CTCAACCTTA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT 
GCCTATGCAC TAGCCAACTT CATCCGGTCT CAAGGCATCA GCTCCCTGAA AGTATGGACT 
AGCCACATGA AGAGGACCAT TCAGACCGCT GAAGCCCTAG GTGTCCCCTA TGAACAGTGG 
AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAAGAGA TGACCTATGA AGAAATTCAG 
GAACACTACC CTGAGGAATT TGCACTACGG GACCAGGATA AATATCGTTA CCGCTATCCC 
AAGGGAGAGT CCTATGAGGA TCTGGTTCAG CGTCTTGAAC CAGTTATAAT GGAGCTAGAA 
CGGCAAGAAA ATGTACTGGT GATCTGTCAC CAGGCTGTCA TGCGGTGCCT CCTGGCATAC 
TTCCTGGATA AAAGTTCAGA TGAGCTGCCC TATCTCAAGT GTCCTCTGCA TACTGTGCTC 
AAACTCACAC CTGTGGCTTA TGGCTGCAGA GTGGAGTCCA TCTACCTGAA TGTGGAGGCT 
GTGAACACAC ACCGGGACAA GCCTGAGAAT GTGGACATCA CCCGTGAAGC TGAGGAAGCC 
TTGGACACTG TACCTGCCCA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256853.1
CDS	3395..4681
Translation	MVIMVGLPARGKTYISTKLTRYLNWIGTPTKVFNLGQYRREAVSYRNYEFFRPDNTEAQLIRKQCALAALKDVHKYLSREEGHVAVFDATNTTRERRSLILQFAKEHGYKVFFIESICNDPEIIAENIKQVKLGSPDYIDCDQEKVLEDFLKRIECYEINYQPLDEELDSHLSYIKIFDVGTRYMVNRVQDHVQSRTAYYLMNIHVTPRSIYLCRHGESELNLRGRIGGDSGLSARGKQYAYALANFIRSQGISSLKVWTSHMKRTIQTAEALGVPYEQWKALNEIDAGVCEEMTYEEIQEHYPEEFALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLHTVLKLTPVAYGCRVESIYLNVEAVNTHRDKPENVDITREAEEALDTVPAHY

Target ORF information:

RefSeq Version	XM_006256791.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (Pfkfb1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256791.3

ORF Insert Sequence:

ATGGTGATCA TGGTGGGTTT ACCAGCTCGA GGCAAGACCT ACATCTCTAC GAAGCTCACA 
CGCTATCTCA ACTGGATAGG AACACCAACT AAAGTGTTTA ATTTAGGTCA GTATCGACGA 
GAGGCAGTGA GTTACAGGAA CTATGAATTC TTTCGCCCAG ACAACACAGA GGCCCAGCTT 
ATCAGGAAGC AGTGTGCTCT AGCAGCCCTA AAGGATGTCC ATAAGTATCT CAGCCGCGAG 
GAAGGTCATG TTGCGGTTTT TGATGCCACC AACACTACCA GAGAACGACG ATCGTTGATT 
CTACAGTTTG CTAAGGAACA TGGTTATAAG GTCTTCTTTA TTGAGTCTAT TTGTAATGAC 
CCCGAAATCA TTGCAGAAAA CATCAAGCAA GTGAAACTTG GTAGTCCTGA TTACATAGAC 
TGTGACCAAG AAAAGGTTTT GGAAGACTTT CTAAAGAGAA TAGAGTGCTA TGAGATCAAC 
TACCAACCTT TGGATGAGGA ATTGGACAGC CACCTGTCCT ACATCAAGAT CTTCGACGTG 
GGCACACGCT ACATGGTAAA TCGAGTGCAG GACCACGTTC AGAGCCGTAC AGCCTACTAC 
CTCATGAACA TCCATGTCAC ACCTCGATCT ATCTACCTAT GCCGCCATGG TGAGAGTGAA 
CTCAACCTTA GAGGCCGCAT TGGAGGTGAC TCTGGCCTCT CAGCTCGGGG CAAGCAGTAT 
GCCTATGCAC TAGCCAACTT CATCCGGTCT CAAGGCATCA GCTCCCTGAA AGTATGGACT 
AGCCACATGA AGAGGACCAT TCAGACCGCT GAAGCCCTAG GTGTCCCCTA TGAACAGTGG 
AAGGCCCTGA ATGAGATTGA TGCGGGTGTC TGTGAAGAGA TGACCTATGA AGAAATTCAG 
GAACACTACC CTGAGGAATT TGCACTACGG GACCAGGATA AATATCGTTA CCGCTATCCC 
AAGGGAGAGT CCTATGAGGA TCTGGTTCAG CGTCTTGAAC CAGTTATAAT GGAGCTAGAA 
CGGCAAGAAA ATGTACTGGT GATCTGTCAC CAGGCTGTCA TGCGGTGCCT CCTGGCATAC 
TTCCTGGATA AAAGTTCAGA TGAGCTGCCC TATCTCAAGT GTCCTCTGCA TACTGTGCTC 
AAACTCACAC CTGTGGCTTA TGGCTGCAGA GTGGAGTCCA TCTACCTGAA TGTGGAGGCT 
GTGAACACAC ACCGGGACAA GCCTGAGAAT GTGGACATCA CCCGTGAAGC TGAGGAAGCC 
TTGGACACTG TACCTGCCCA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes.
The Biochemical journal474(16)2785-2801(2017 08)
Gibb AA,Lorkiewicz PK,Zheng YT,Zhang X,Bhatnagar A,Jones SP,Hill BG

Identification of a novel Pfkfb1 mRNA variant in rat fetal liver.
Biochemical and biophysical research communications431(1)36-40(2013 Feb)
Cosin-Roger J,Vernia S,Alvarez MS,Cucarella C,Bosc? L,Martin-Sanz P,Fern?ndez-Alvarez AJ,Casado M

The mechanism of calcium-induced inhibition of muscle fructose 1,6-bisphosphatase and destabilization of glyconeogenic complex.
PloS one8(10)e76669(2013)
Rakus D,Gizak A,Kasprzak AA,Zarzycki M,Maciaszczyk-Dziubinska E,Dzugaj A

Dimer interface rearrangement of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase rat liver isoenzyme by cAMP-dependent Ser-32 phosphorylation.
FEBS letters586(10)1419-25(2012 May)
Langer S,Okar DA,Schultz J,Lenzen S,Baltrusch S

The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate.
Archives of biochemistry and biophysics438(2)125-36(2005 Jun)
Manes NP,El-Maghrabi MR

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Pfkfb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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