Arfgap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arfgap1
Entrez Gene ID	246310
Full Name	ADP-ribosylation factor GTPase activating protein 1
Synonyms	Arf GAP1,Arf1gap
General protein information	Preferred Names ADP-ribosylation factor GTPase activating protein 1 Names ADP-ribosylation factor GTPase-activating protein 1 ADP-ribosylation factor 1 GTPase-activating protein ARF GAP 1 ARF1 GAP ARF1-directed GTPase-activating protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q43
Summary	GTPase activating protein for ADP-ribosylation factor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006235731.3	XP_006235793.1	ADP-ribosylation factor GTPase-activating protein 1 isoform X1
XM_017591464.1	XP_017446953.1	ADP-ribosylation factor GTPase-activating protein 1 isoform X2
XM_017591465.1	XP_017446954.1	ADP-ribosylation factor GTPase-activating protein 1 isoform X4
NM_145090.3	NP_659558.1	ADP-ribosylation factor GTPase-activating protein 1
XM_006235732.2	XP_006235794.1	ADP-ribosylation factor GTPase-activating protein 1 isoform X1
XM_006235734.3	XP_006235796.1	ADP-ribosylation factor GTPase-activating protein 1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARFGAP1	NP_060679.1
Saccharomyces cerevisiae (baker's yeast)	GCS1	NP_010055.1
Pan troglodytes (chimpanzee)	ARFGAP1	XP_001148867.1
Gallus gallus (chicken)	ARFGAP1	NP_001026028.1
Magnaporthe oryzae (rice blast fungus)	MGG_01745	XP_003714742.1
Neurospora crassa	NCU07734	XP_961984.1
Bos taurus (cattle)	ARFGAP1	NP_001068732.1
Rattus norvegicus (Norway rat)	Arfgap1	NP_659558.1
Kluyveromyces lactis	KLLA0D18942g	XP_453901.1
Eremothecium gossypii	AGOS_ABL164C	NP_982783.1
	ARFGAP1	XP_002798103.1
Canis lupus familiaris (dog)	ARFGAP1	XP_005635338.1
Danio rerio (zebrafish)	arfgap1	NP_001007304.1
Schizosaccharomyces pombe (fission yeast)	SPCC622.14	NP_588186.2
Mus musculus (house mouse)	Arfgap1	NP_665703.2
Xenopus tropicalis (tropical clawed frog)	arfgap1	NP_001123813.1

Gene Ontology
Bibliography

Related articles in PubMed

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events.
Schultz ML, Tecedor L, Stein CS, Stamnes MA, Davidson BL
PloS one9(5)e96647(2014)

ArfGAP1 is a GTPase activating protein for LRRK2: reciprocal regulation of ArfGAP1 by LRRK2.
Xiong Y, Yuan C, Chen R, Dawson TM, Dawson VL
The Journal of neuroscience : the official journal of the Society for Neuroscience32(11)3877-86(2012 Mar)

GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.
Stafa K, Trancikova A, Webber PJ, Glauser L, West AB, Moore DJ
PLoS genetics8(2)e1002526(2012)

ArfGAP1 interacts with coat proteins through tryptophan-based motifs.
Rawet M, Levi-Tal S, Szafer-Glusman E, Parnis A, Cassel D
Biochemical and biophysical research communications394(3)553-7(2010 Apr)

Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking.
Weimer C, Beck R, Eckert P, Reckmann I, Moelleken J, Br?gger B, Wieland F
The Journal of cell biology183(4)725-35(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings point to mechanistic differences between ArfGAP1 and the other ArfGAPs known to function in the COPI system.
Title: ArfGAP1 interacts with coat proteins through tryptophan-based motifs.

Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking
Title: Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking.

ALPS1 is the primary determinant of the interaction of ArfGAP1 with lipid membranes and that ALPS2 reinforces this interaction 40-fold; ArfGAP1 responds to a wide range of membrane curvature and adapts to lipid membranes of various acyl chain compositions
Title: Two lipid-packing sensor motifs contribute to the sensitivity of ArfGAP1 to membrane curvature.

Data show that casein kinase I is required for protein traffic through the early secretory pathway and can modulate the amount of ADP-ribosylation factor-GTPase activating protein (ARF-GAP1) that can bind to membranes.
Title: Casein kinase I regulates membrane binding by ARF GAP1.

The following Arfgap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arfgap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02557	NM_145090.3 Latest version!	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa26781	XM_006235731.3 Latest version!	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa26781	XM_006235732.2 Latest version!	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa26783	XM_006235734.3 Latest version!	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa60261	XM_017591464.1 Latest version!	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa60262	XM_017591465.1 Latest version!	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arfgap1 ( NM_145090.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_659558.1 Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), mRNA.

Price & Availability↑

CloneID	ORa02557
Clone ID Related Accession (Same CDS sequence)	NM_145090.3
Accession Version	NM_145090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor GTPase-activating protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U35776.1. On Aug 12, 2007 this sequence version replaced NM_145090.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U35776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GATCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGGCT 
TCGGAGTTGG GCCACAGCCT GAATGAGAAT GTTCTCAAGC CTGCACAGGA GAAGGTGAAA 
GAGGGAAGGA TTTTTGATGA TGTGTCCAGT GGGGTCTCTC AGTTGGCATC CAAGGTCCAG 
GGAGTTGGCA GTAAGGGATG GCGTGATGTC ACGACCTTCT TTTCTGGGAA AGCTGAAGAC 
ACTTCAGACA GACCCTTAGA GGGCCACAGC TACCAGAACA GCAGTGGAGA CAACTCTCAG 
AACAGCACCA TAGACCAGAG CTTCTGGGAG ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC 
AAGTCCCCAA GCAGTGACAG CTGGACCTGC GCAGATGCCT CAACGGGAAG GAGGAGCTCG 
GACAGCTGGG ACATTTGGGG CTCAGGTTCT GCATCCAACA ACAAGAACAG CAATAGCGAT 
GGTTGGGAGA GCTGGGAGGG AGCCAGTGGG GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA 
GCTCCATCTA CAGCCGCCGA CGAGGGCTGG GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_659558.1
CDS	284..1531
Translation	MASPRTRKVLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLEAQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESSPAQNWTPPQPKTLQFTAHRPAGQPQNVTTSGDKAFEDWLNDDLGSYQGAQENRYVGFGNTVPPQKREDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGRIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEDTSDRPLEGHSYQNSSGDNSQNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGSGSASNNKNSNSDGWESWEGASGEGRAKATKKAAPSTAADEGWDNQNW

Target ORF information:

RefSeq Version	NM_145090.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145090.3

ORF Insert Sequence:

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GATCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGGCT 
TCGGAGTTGG GCCACAGCCT GAATGAGAAT GTTCTCAAGC CTGCACAGGA GAAGGTGAAA 
GAGGGAAGGA TTTTTGATGA TGTGTCCAGT GGGGTCTCTC AGTTGGCATC CAAGGTCCAG 
GGAGTTGGCA GTAAGGGATG GCGTGATGTC ACGACCTTCT TTTCTGGGAA AGCTGAAGAC 
ACTTCAGACA GACCCTTAGA GGGCCACAGC TACCAGAACA GCAGTGGAGA CAACTCTCAG 
AACAGCACCA TAGACCAGAG CTTCTGGGAG ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC 
AAGTCCCCAA GCAGTGACAG CTGGACCTGC GCAGATGCCT CAACGGGAAG GAGGAGCTCG 
GACAGCTGGG ACATTTGGGG CTCAGGTTCT GCATCCAACA ACAAGAACAG CAATAGCGAT 
GGTTGGGAGA GCTGGGAGGG AGCCAGTGGG GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA 
GCTCCATCTA CAGCCGCCGA CGAGGGCTGG GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgap1 ( XM_006235731.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235793.1 Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa26781
Clone ID Related Accession (Same CDS sequence)	XM_006235731.3 , XM_006235732.2
Accession Version	XM_006235731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGTTT 
TGGGGTTACA AGCAGCAGTC AGAGCCGGCT TCGGAGTTGG GCCACAGCCT GAATGAGAAT 
GTTCTCAAGC CTGCACAGGA GAAGGTGAAA GAGGGAAGGA TTTTTGATGA TGTGTCCAGT 
GGGGTCTCTC AGTTGGCATC CAAGGTCCAG GGAGTTGGCA GTAAGGGATG GCGTGATGTC 
ACGACCTTTT TTTCTGGGAA AGCTGAAGAC ACTTCAGACA GACCCTTAGA GGGCCACAGC 
TACCAGAACA GCAGTGGAGA CAACTCTCAG AACAGCACCA TAGACCAGAG CTTCTGGGAG 
ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC AAGTCCCCAA GCAGTGACAG CTGGACCTGC 
GCAGATGCCT CAACGGGAAG GAGGAGCTCG GACAGCTGGG ACATTTGGGG CTCAGGTTCT 
GCATCCAACA ACAAGAACAG CAATAGCGAT GGCTGGGAGA GCTGGGAGGG AGCCAGTGGG 
GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA GCTCCATCTA CAGCCGCCGA CGAGGGCTGG 
GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235793.1
CDS	385..1662
Translation	MASPRTRKVLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLEAQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESSPAQNWTPPQPKTLQFTAHRPAGQPQNVTTSGDKAFEDWLNDDLGSYQGAQENRYVGFGNTVPPQKREDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATKFGSQASQKFWGYKQQSEPASELGHSLNENVLKPAQEKVKEGRIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEDTSDRPLEGHSYQNSSGDNSQNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGSGSASNNKNSNSDGWESWEGASGEGRAKATKKAAPSTAADEGWDNQNW

Target ORF information:

RefSeq Version	XM_006235731.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235731.3

ORF Insert Sequence:

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGTTT 
TGGGGTTACA AGCAGCAGTC AGAGCCGGCT TCGGAGTTGG GCCACAGCCT GAATGAGAAT 
GTTCTCAAGC CTGCACAGGA GAAGGTGAAA GAGGGAAGGA TTTTTGATGA TGTGTCCAGT 
GGGGTCTCTC AGTTGGCATC CAAGGTCCAG GGAGTTGGCA GTAAGGGATG GCGTGATGTC 
ACGACCTTTT TTTCTGGGAA AGCTGAAGAC ACTTCAGACA GACCCTTAGA GGGCCACAGC 
TACCAGAACA GCAGTGGAGA CAACTCTCAG AACAGCACCA TAGACCAGAG CTTCTGGGAG 
ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC AAGTCCCCAA GCAGTGACAG CTGGACCTGC 
GCAGATGCCT CAACGGGAAG GAGGAGCTCG GACAGCTGGG ACATTTGGGG CTCAGGTTCT 
GCATCCAACA ACAAGAACAG CAATAGCGAT GGCTGGGAGA GCTGGGAGGG AGCCAGTGGG 
GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA GCTCCATCTA CAGCCGCCGA CGAGGGCTGG 
GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgap1 ( XM_006235732.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235794.1 Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa26781
Clone ID Related Accession (Same CDS sequence)	XM_006235731.3 , XM_006235732.2
Accession Version	XM_006235732.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235732.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGTTT 
TGGGGTTACA AGCAGCAGTC AGAGCCGGCT TCGGAGTTGG GCCACAGCCT GAATGAGAAT 
GTTCTCAAGC CTGCACAGGA GAAGGTGAAA GAGGGAAGGA TTTTTGATGA TGTGTCCAGT 
GGGGTCTCTC AGTTGGCATC CAAGGTCCAG GGAGTTGGCA GTAAGGGATG GCGTGATGTC 
ACGACCTTTT TTTCTGGGAA AGCTGAAGAC ACTTCAGACA GACCCTTAGA GGGCCACAGC 
TACCAGAACA GCAGTGGAGA CAACTCTCAG AACAGCACCA TAGACCAGAG CTTCTGGGAG 
ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC AAGTCCCCAA GCAGTGACAG CTGGACCTGC 
GCAGATGCCT CAACGGGAAG GAGGAGCTCG GACAGCTGGG ACATTTGGGG CTCAGGTTCT 
GCATCCAACA ACAAGAACAG CAATAGCGAT GGCTGGGAGA GCTGGGAGGG AGCCAGTGGG 
GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA GCTCCATCTA CAGCCGCCGA CGAGGGCTGG 
GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235794.1
CDS	325..1602
Translation	MASPRTRKVLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLEAQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESSPAQNWTPPQPKTLQFTAHRPAGQPQNVTTSGDKAFEDWLNDDLGSYQGAQENRYVGFGNTVPPQKREDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATKFGSQASQKFWGYKQQSEPASELGHSLNENVLKPAQEKVKEGRIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEDTSDRPLEGHSYQNSSGDNSQNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGSGSASNNKNSNSDGWESWEGASGEGRAKATKKAAPSTAADEGWDNQNW

Target ORF information:

RefSeq Version	XM_006235732.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235732.2

ORF Insert Sequence:

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGTTT 
TGGGGTTACA AGCAGCAGTC AGAGCCGGCT TCGGAGTTGG GCCACAGCCT GAATGAGAAT 
GTTCTCAAGC CTGCACAGGA GAAGGTGAAA GAGGGAAGGA TTTTTGATGA TGTGTCCAGT 
GGGGTCTCTC AGTTGGCATC CAAGGTCCAG GGAGTTGGCA GTAAGGGATG GCGTGATGTC 
ACGACCTTTT TTTCTGGGAA AGCTGAAGAC ACTTCAGACA GACCCTTAGA GGGCCACAGC 
TACCAGAACA GCAGTGGAGA CAACTCTCAG AACAGCACCA TAGACCAGAG CTTCTGGGAG 
ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC AAGTCCCCAA GCAGTGACAG CTGGACCTGC 
GCAGATGCCT CAACGGGAAG GAGGAGCTCG GACAGCTGGG ACATTTGGGG CTCAGGTTCT 
GCATCCAACA ACAAGAACAG CAATAGCGAT GGCTGGGAGA GCTGGGAGGG AGCCAGTGGG 
GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA GCTCCATCTA CAGCCGCCGA CGAGGGCTGG 
GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgap1 ( XM_006235734.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235796.1 Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa26783
Clone ID Related Accession (Same CDS sequence)	XM_006235734.3
Accession Version	XM_006235734.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor GTPase-activating protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGGCT 
TCGGAGTTGG GCCACAGCCT GAATGAGAAT GTTCTCAAGC CTGCACAGGA GAAGGGAGTT 
GGCAGTAAGG GATGGCGTGA TGTCACGACC TTTTTTTCTG GGAAAGCTGA AGACACTTCA 
GACAGACCCT TAGAGGGCCA CAGCTACCAG AACAGCAGTG GAGACAACTC TCAGAACAGC 
ACCATAGACC AGAGCTTCTG GGAGACCTTC GGGAGTGCTG AGCCCCCCAA GGCCAAGTCC 
CCAAGCAGTG ACAGCTGGAC CTGCGCAGAT GCCTCAACGG GAAGGAGGAG CTCGGACAGC 
TGGGACATTT GGGGCTCAGG TTCTGCATCC AACAACAAGA ACAGCAATAG CGATGGCTGG 
GAGAGCTGGG AGGGAGCCAG TGGGGAGGGA AGGGCAAAGG CTACCAAGAA GGCAGCTCCA 
TCTACAGCCG CCGACGAGGG CTGGGACAAC CAGAACTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235796.1
CDS	590..1771
Translation	MASPRTRKVLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLEAQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESSPAQNWTPPQPKTLQFTAHRPAGQPQNVTTSGDKAFEDWLNDDLGSYQGAQENRYVGFGNTVPPQKREDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKGVGSKGWRDVTTFFSGKAEDTSDRPLEGHSYQNSSGDNSQNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGSGSASNNKNSNSDGWESWEGASGEGRAKATKKAAPSTAADEGWDNQNW

Target ORF information:

RefSeq Version	XM_006235734.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235734.3

ORF Insert Sequence:

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGGCT 
TCGGAGTTGG GCCACAGCCT GAATGAGAAT GTTCTCAAGC CTGCACAGGA GAAGGGAGTT 
GGCAGTAAGG GATGGCGTGA TGTCACGACC TTTTTTTCTG GGAAAGCTGA AGACACTTCA 
GACAGACCCT TAGAGGGCCA CAGCTACCAG AACAGCAGTG GAGACAACTC TCAGAACAGC 
ACCATAGACC AGAGCTTCTG GGAGACCTTC GGGAGTGCTG AGCCCCCCAA GGCCAAGTCC 
CCAAGCAGTG ACAGCTGGAC CTGCGCAGAT GCCTCAACGG GAAGGAGGAG CTCGGACAGC 
TGGGACATTT GGGGCTCAGG TTCTGCATCC AACAACAAGA ACAGCAATAG CGATGGCTGG 
GAGAGCTGGG AGGGAGCCAG TGGGGAGGGA AGGGCAAAGG CTACCAAGAA GGCAGCTCCA 
TCTACAGCCG CCGACGAGGG CTGGGACAAC CAGAACTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgap1 ( XM_017591464.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446953.1 Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa60261
Clone ID Related Accession (Same CDS sequence)	XM_017591464.1
Accession Version	XM_017591464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor GTPase-activating protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGGCT 
TCGGAGTTGG GCCACAGCCT GAATGAGAAT GTTCTCAAGC CTGCACAGGA GAAGGTGAAA 
GAGGGAAGGA TTTTTGATGA TGTGTCCAGT GGGGTCTCTC AGTTGGCATC CAAGGTCCAG 
GGAGTTGGCA GTAAGGGATG GCGTGATGTC ACGACCTTTT TTTCTGGGAA AGCTGAAGAC 
ACTTCAGACA GACCCTTAGA GGGCCACAGC TACCAGAACA GCAGTGGAGA CAACTCTCAG 
AACAGCACCA TAGACCAGAG CTTCTGGGAG ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC 
AAGTCCCCAA GCAGTGACAG CTGGACCTGC GCAGATGCCT CAACGGGAAG GAGGAGCTCG 
GACAGCTGGG ACATTTGGGG CTCAGGTTCT GCATCCAACA ACAAGAACAG CAATAGCGAT 
GGCTGGGAGA GCTGGGAGGG AGCCAGTGGG GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA 
GCTCCATCTA CAGCCGCCGA CGAGGGCTGG GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446953.1
CDS	590..1837
Translation	MASPRTRKVLKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKDIELEKMKAGGNAKFREFLEAQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESSPAQNWTPPQPKTLQFTAHRPAGQPQNVTTSGDKAFEDWLNDDLGSYQGAQENRYVGFGNTVPPQKREDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGRIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEDTSDRPLEGHSYQNSSGDNSQNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGSGSASNNKNSNSDGWESWEGASGEGRAKATKKAAPSTAADEGWDNQNW

Target ORF information:

RefSeq Version	XM_017591464.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591464.1

ORF Insert Sequence:

ATGGCCAGCC CAAGAACCAG AAAAGTTCTT AAGGAAGTCC GGGCACAGGA CGAGAATAAT 
GTTTGCTTTG AGTGTGGTGC GTTCAATCCT CAGTGGGTCA GCGTGACCTA TGGCATCTGG 
ATCTGCCTGG AATGCTCTGG GAGACACCGT GGGCTTGGGG TGCATCTCAG CTTCGTGCGC 
TCTGTGACAA TGGACAAGTG GAAGGACATT GAACTGGAGA AGATGAAAGC TGGTGGGAAT 
GCTAAGTTCC GAGAGTTCCT GGAGGCACAG GACGACTATG AGCCTAGCTG GTCACTGCAG 
GACAAGTATA GCAGCAGAGC TGCAGCGCTC TTCAGGGATA AGGTGGCTAC TTTGGCAGAA 
GGTAAAGAAT GGTCTCTGGA GTCATCGCCT GCCCAGAACT GGACCCCACC TCAGCCCAAG 
ACGCTGCAGT TCACTGCCCA CCGACCCGCT GGCCAGCCAC AGAATGTAAC TACCTCTGGG 
GACAAGGCCT TTGAGGATTG GCTGAATGAT GACCTGGGTT CCTATCAGGG TGCTCAGGAG 
AATCGCTATG TGGGGTTTGG GAACACAGTG CCACCTCAGA AGAGAGAAGA TGACTTCCTC 
AACAGCGCCA TGTCATCTCT GTACTCGGGC TGGAGCAGTT TTACCACTGG GGCGAGCAAG 
TTTGCGTCTG CAGCAAAGGA GGGTGCTACA AAATTTGGAT CTCAAGCAAG TCAAAAGGCT 
TCGGAGTTGG GCCACAGCCT GAATGAGAAT GTTCTCAAGC CTGCACAGGA GAAGGTGAAA 
GAGGGAAGGA TTTTTGATGA TGTGTCCAGT GGGGTCTCTC AGTTGGCATC CAAGGTCCAG 
GGAGTTGGCA GTAAGGGATG GCGTGATGTC ACGACCTTTT TTTCTGGGAA AGCTGAAGAC 
ACTTCAGACA GACCCTTAGA GGGCCACAGC TACCAGAACA GCAGTGGAGA CAACTCTCAG 
AACAGCACCA TAGACCAGAG CTTCTGGGAG ACCTTCGGGA GTGCTGAGCC CCCCAAGGCC 
AAGTCCCCAA GCAGTGACAG CTGGACCTGC GCAGATGCCT CAACGGGAAG GAGGAGCTCG 
GACAGCTGGG ACATTTGGGG CTCAGGTTCT GCATCCAACA ACAAGAACAG CAATAGCGAT 
GGCTGGGAGA GCTGGGAGGG AGCCAGTGGG GAGGGAAGGG CAAAGGCTAC CAAGAAGGCA 
GCTCCATCTA CAGCCGCCGA CGAGGGCTGG GACAACCAGA ACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arfgap1 ( XM_017591465.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446954.1 Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa60262
Clone ID Related Accession (Same CDS sequence)	XM_017591465.1
Accession Version	XM_017591465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ADP-ribosylation factor GTPase-activating protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGTT CCGAGAGTTC CTGGAGGCAC AGGACGACTA TGAGCCTAGC TGGTCACTGC 
AGGACAAGTA TAGCAGCAGA GCTGCAGCGC TCTTCAGGGA TAAGACCCGC TGGCCAGCCA 
CAGAATGTAA CTACCTCTGG GGACAAGGCC TTTGAGGATT GGCTGAATGA TGACCTGGGT 
TCCTATCAGG GTGCTCAGGA GAATCGCTAT GTGGGGTTTG GGAACACAGT GCCACCTCAG 
AAGAGAGAAG ATGACTTCCT CAACAGCGCC ATGTCATCTC TGTACTCGGG CTGGAGCAGT 
TTTACCACTG GGGCGAGCAA GTTTGCGTCT GCAGCAAAGG AGGGTGCTAC AAAATTTGGA 
TCTCAAGCAA GTCAAAAGGC TTCGGAGTTG GGCCACAGCC TGAATGAGAA TGTTCTCAAG 
CCTGCACAGG AGAAGGTGAA AGAGGGAAGG ATTTTTGATG ATGTGTCCAG TGGGGTCTCT 
CAGTTGGCAT CCAAGGTCCA GGGAGTTGGC AGTAAGGGAT GGCGTGATGT CACGACCTTT 
TTTTCTGGGA AAGCTGAAGA CACTTCAGAC AGACCCTTAG AGGGCCACAG CTACCAGAAC 
AGCAGTGGAG ACAACTCTCA GAACAGCACC ATAGACCAGA GCTTCTGGGA GACCTTCGGG 
AGTGCTGAGC CCCCCAAGGC CAAGTCCCCA AGCAGTGACA GCTGGACCTG CGCAGATGCC 
TCAACGGGAA GGAGGAGCTC GGACAGCTGG GACATTTGGG GCTCAGGTTC TGCATCCAAC 
AACAAGAACA GCAATAGCGA TGGCTGGGAG AGCTGGGAGG GAGCCAGTGG GGAGGGAAGG 
GCAAAGGCTA CCAAGAAGGC AGCTCCATCT ACAGCCGCCG ACGAGGGCTG GGACAACCAG 
AACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446954.1
CDS	1..909
Translation	MLSSESSWRHRTTMSLAGHCRTSIAAELQRSSGIRPAGQPQNVTTSGDKAFEDWLNDDLGSYQGAQENRYVGFGNTVPPQKREDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGRIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAEDTSDRPLEGHSYQNSSGDNSQNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGSGSASNNKNSNSDGWESWEGASGEGRAKATKKAAPSTAADEGWDNQNW

Target ORF information:

RefSeq Version	XM_017591465.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADP-ribosylation factor GTPase activating protein 1 (Arfgap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591465.1

ORF Insert Sequence:

ATGCTAAGTT CCGAGAGTTC CTGGAGGCAC AGGACGACTA TGAGCCTAGC TGGTCACTGC 
AGGACAAGTA TAGCAGCAGA GCTGCAGCGC TCTTCAGGGA TAAGACCCGC TGGCCAGCCA 
CAGAATGTAA CTACCTCTGG GGACAAGGCC TTTGAGGATT GGCTGAATGA TGACCTGGGT 
TCCTATCAGG GTGCTCAGGA GAATCGCTAT GTGGGGTTTG GGAACACAGT GCCACCTCAG 
AAGAGAGAAG ATGACTTCCT CAACAGCGCC ATGTCATCTC TGTACTCGGG CTGGAGCAGT 
TTTACCACTG GGGCGAGCAA GTTTGCGTCT GCAGCAAAGG AGGGTGCTAC AAAATTTGGA 
TCTCAAGCAA GTCAAAAGGC TTCGGAGTTG GGCCACAGCC TGAATGAGAA TGTTCTCAAG 
CCTGCACAGG AGAAGGTGAA AGAGGGAAGG ATTTTTGATG ATGTGTCCAG TGGGGTCTCT 
CAGTTGGCAT CCAAGGTCCA GGGAGTTGGC AGTAAGGGAT GGCGTGATGT CACGACCTTT 
TTTTCTGGGA AAGCTGAAGA CACTTCAGAC AGACCCTTAG AGGGCCACAG CTACCAGAAC 
AGCAGTGGAG ACAACTCTCA GAACAGCACC ATAGACCAGA GCTTCTGGGA GACCTTCGGG 
AGTGCTGAGC CCCCCAAGGC CAAGTCCCCA AGCAGTGACA GCTGGACCTG CGCAGATGCC 
TCAACGGGAA GGAGGAGCTC GGACAGCTGG GACATTTGGG GCTCAGGTTC TGCATCCAAC 
AACAAGAACA GCAATAGCGA TGGCTGGGAG AGCTGGGAGG GAGCCAGTGG GGAGGGAAGG 
GCAAAGGCTA CCAAGAAGGC AGCTCCATCT ACAGCCGCCG ACGAGGGCTG GGACAACCAG 
AACTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLN3 deficient cells display defects in the ARF1-Cdc42 pathway and actin-dependent events.
PloS one9(5)e96647(2014)
Schultz ML,Tecedor L,Stein CS,Stamnes MA,Davidson BL

ArfGAP1 is a GTPase activating protein for LRRK2: reciprocal regulation of ArfGAP1 by LRRK2.
The Journal of neuroscience : the official journal of the Society for Neuroscience32(11)3877-86(2012 Mar)
Xiong Y,Yuan C,Chen R,Dawson TM,Dawson VL

GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.
PLoS genetics8(2)e1002526(2012)
Stafa K,Trancikova A,Webber PJ,Glauser L,West AB,Moore DJ

ArfGAP1 interacts with coat proteins through tryptophan-based motifs.
Biochemical and biophysical research communications394(3)553-7(2010 Apr)
Rawet M,Levi-Tal S,Szafer-Glusman E,Parnis A,Cassel D

Differential roles of ArfGAP1, ArfGAP2, and ArfGAP3 in COPI trafficking.
The Journal of cell biology183(4)725-35(2008 Nov)
Weimer C,Beck R,Eckert P,Reckmann I,Moelleken J,Br?gger B,Wieland F

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Arfgap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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