Trib3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trib3
Entrez Gene ID	246273
Full Name	tribbles pseudokinase 3
Synonyms	NIPK
General protein information	Preferred Names tribbles pseudokinase 3 Names tribbles homolog 3 Neuronal cell death Inducible Putative Kinase TRB-3 kinase neuronal cell death-inducible putative kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41
Summary	putative kinase; expression upregulated by NGF-depletion induced programmed cell death [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_144755.2	NP_653356.1	tribbles homolog 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TRIB3	XP_003953728.1
Canis lupus familiaris (dog)	TRIB3	XP_542943.2
Rattus norvegicus (Norway rat)	Trib3	NP_653356.1
Homo sapiens (human)	TRIB3	NP_066981.2
Mus musculus (house mouse)	Trib3	NP_780302.2
Danio rerio (zebrafish)	trib3	NP_998034.1
	TRIB3	XP_002798226.1
Bos taurus (cattle)	TRIB3	NP_001069571.1

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of TRB3 protects neurons against apoptosis induced by global cerebral ischemia and reperfusion injury in rats.
Wei K, Wan L, Liu J, Zhang B, Li X, Zhang Y, Zhang C, Yao W
Neuroscience360118-127(2017 Sep)

TRB3 mediates advanced glycation end product-induced apoptosis of pancreatic ?-cells through the protein kinase C ? pathway.
Wang M, Zhang W, Xu S, Peng L, Wang Z, Liu H, Fang Q, Deng T, Men X, Lou J
International journal of molecular medicine40(1)130-136(2017 Jul)

TRB3 gene silencing activates AMPK in adipose tissue with beneficial metabolic effects in obese and diabetic rats.
Sun X, Song M, Wang H, Zhou H, Wang F, Li Y, Zhang Y, Zhang W, Zhong M, Ti Y
Biochemical and biophysical research communications488(1)22-28(2017 06)

Silencing of TRB3 Ameliorates Diabetic Tubule Interstitial Nephropathy via PI3K/AKT Signaling in Rats.
Ma Y, Chen F, Yang S, Duan Y, Sun Z, Shi J
Medical science monitor : international medical journal of experimental and clinical research232816-2824(2017 Jun)

Nupr1 Modulates Methamphetamine-Induced Dopaminergic Neuronal Apoptosis and Autophagy through CHOP-Trib3-Mediated Endoplasmic Reticulum Stress Signaling Pathway.
Xu X, Huang E, Tai Y, Zhao X, Chen X, Chen C, Chen R, Liu C, Lin Z, Wang H, Xie WB
Frontiers in molecular neuroscience10203(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The TRB3 silencing alleviated adipocytes insulin resistance. With TRB3 gene silencing, protein levels of IRS-1, GLUT4 and p-AMPK were significantly increased in adipocytes. TRB3 gene silencing decreased blood glucose, ameliorated insulin sensitivity and adipose tissue remodeling in diabetic rats. TRB3 silencing decreased triglyceride, increased glycogen simultaneously in diabetic epididymal and brown adipose tissues (BAT)
Title: TRB3 gene silencing activates AMPK in adipose tissue with beneficial metabolic effects in obese and diabetic rats.

results suggest that a self-amplifying feed-forward loop among Trib3, Akt, and FoxO1 in Abeta-treated neurons induces both apoptosis and autophagy, culminating in neuron death
Title: Tribbles Pseudokinase 3 Induces Both Apoptosis and Autophagy in Amyloid-?-induced Neuronal Death.

Findings suggest a role for TRIB3 in the development of acute insulin resistance following injury
Title: Skeletal muscle atrogene expression and insulin resistance in a rat model of polytrauma.

Trib3 may be associated with the pathogenic mechanism of demyelination in dmy rats.
Title: Enhanced Expression of Trib3 during the Development of Myelin Breakdown in dmy Myelin Mutant Rats.

Diabetic rats displayed increased aortic medial thickness, excessive collagen deposition, diminished elastic fibres and reduced vascular compliance with Tribbles 3 overexpression. Trib3 silencing reversed pathological aortic remodeling in diabetic rats.
Title: Tribbles 3: A potential player in diabetic aortic remodelling.

The following Trib3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trib3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12765	NM_144755.2 Latest version!	Rattus norvegicus tribbles pseudokinase 3 (Trib3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trib3 ( NM_144755.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_653356.1 Rattus norvegicus tribbles pseudokinase 3 (Trib3), mRNA.

Price & Availability↑

CloneID	ORa12765
Clone ID Related Accession (Same CDS sequence)	NM_144755.2
Accession Version	NM_144755.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	tribbles homolog 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091120.1. On Jun 3, 2007 this sequence version replaced NM_144755.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC091120.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGAGCCA CATCTCTGGC TGCTTCTGCC GATGTTCCCT GCAGGAAGAA ACCGTTGGAG 
TTTGATGACA ACATTGATGT CGAGTGTCCA GTCTTAAAAC GAGTGAGAGA TGAGCCTGAG 
CCTGGACCAA CCCCCAGCCT ACCTCCCGCC TCAGACTTGT CACCTGCTGT GGCCCCTGCA 
ACCCGGCTGG GGCCCTATAT CCTTTTGGAA CGAGAGCAAG GCAACTGCAC CTATCGGGCC 
CTGCACTGCC CCACAGGCAC AGAGTACACC TGCAAGGTGT ATCCCGCCAG CGAGGCCCAG 
GCGGTGCTGG CACCGTATGC AAGACTGCCT ACCCACCAGC ACGTGGCCCG TCCCACAGAG 
GTCCTGCTGG GCTCTCAGCT CCTATACACC TTTTTCACGA AGACCCATGG GGACTTGCAC 
AGTCTGGTGC GCAGCCGCCG CGGTATCCCC GAGCCCGAGG CTGCCGCGCT TTTCCGACAG 
ATGGCTAGTG CGGTGGCACA CTGCCACAAG CACGGGCTCA TCTTGCGCGA CCTCAAGTTG 
CGTCGATTTG TCTTCAGCAA CTGTGAGAGG ACGAAGTTGG TGCTGGAGAA CCTGGAGGAT 
GCCTGTGTGA TGACTGGACC AGACGACTCT CTGTGGGACA AGCACGCGTG CCCTGCCTAT 
GTGGGACCAG AGATACTCAG CTCCCGGCCA TCCTACTCCG GCAGAGCGGC TGATGTCTGG 
AGCCTGGGCG TGGCGCTCTT CACCATGCTG GCTGGCCGAT ACCCGTTCCA GGACTCTGAA 
CCAGCCCTGC TCTTTGGCAA GATCCGTAGA GGGACCTTTG CCCTGCCTGA GGGCCTATCA 
GCCTCTGCTC GATGCCTCAT CCGCTGTCTC CTCCGCAGGG AGCCTTCAGA GCGACTTGTG 
GCCCTGGGAA TCCTGCTACA TCCCTGGTTG AGGGAGGATT GCAGCCAAGT CTCTCCTCCA 
CGGTCTGACC GAAGGGAGAT GGACCAGGTT GTCCCAGATG GGCCACAGCT GGAGGAGGCA 
GAGGAAGGGG AGGTGGGGCT GTATGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653356.1
CDS	169..1218
Translation	MRATSLAASADVPCRKKPLEFDDNIDVECPVLKRVRDEPEPGPTPSLPPASDLSPAVAPATRLGPYILLEREQGNCTYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSQLLYTFFTKTHGDLHSLVRSRRGIPEPEAAALFRQMASAVAHCHKHGLILRDLKLRRFVFSNCERTKLVLENLEDACVMTGPDDSLWDKHACPAYVGPEILSSRPSYSGRAADVWSLGVALFTMLAGRYPFQDSEPALLFGKIRRGTFALPEGLSASARCLIRCLLRREPSERLVALGILLHPWLREDCSQVSPPRSDRREMDQVVPDGPQLEEAEEGEVGLYG

Target ORF information:

RefSeq Version	NM_144755.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tribbles pseudokinase 3 (Trib3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144755.2

ORF Insert Sequence:

ATGCGAGCCA CATCTCTGGC TGCTTCTGCC GATGTTCCCT GCAGGAAGAA ACCGTTGGAG 
TTTGATGACA ACATTGATGT CGAGTGTCCA GTCTTAAAAC GAGTGAGAGA TGAGCCTGAG 
CCTGGACCAA CCCCCAGCCT ACCTCCCGCC TCAGACTTGT CACCTGCTGT GGCCCCTGCA 
ACCCGGCTGG GGCCCTATAT CCTTTTGGAA CGAGAGCAAG GCAACTGCAC CTATCGGGCC 
CTGCACTGCC CCACAGGCAC AGAGTACACC TGCAAGGTGT ATCCCGCCAG CGAGGCCCAG 
GCGGTGCTGG CACCGTATGC AAGACTGCCT ACCCACCAGC ACGTGGCCCG TCCCACAGAG 
GTCCTGCTGG GCTCTCAGCT CCTATACACC TTTTTCACGA AGACCCATGG GGACTTGCAC 
AGTCTGGTGC GCAGCCGCCG CGGTATCCCC GAGCCCGAGG CTGCCGCGCT TTTCCGACAG 
ATGGCTAGTG CGGTGGCACA CTGCCACAAG CACGGGCTCA TCTTGCGCGA CCTCAAGTTG 
CGTCGATTTG TCTTCAGCAA CTGTGAGAGG ACGAAGTTGG TGCTGGAGAA CCTGGAGGAT 
GCCTGTGTGA TGACTGGACC AGACGACTCT CTGTGGGACA AGCACGCGTG CCCTGCCTAT 
GTGGGACCAG AGATACTCAG CTCCCGGCCA TCCTACTCCG GCAGAGCGGC TGATGTCTGG 
AGCCTGGGCG TGGCGCTCTT CACCATGCTG GCTGGCCGAT ACCCGTTCCA GGACTCTGAA 
CCAGCCCTGC TCTTTGGCAA GATCCGTAGA GGGACCTTTG CCCTGCCTGA GGGCCTATCA 
GCCTCTGCTC GATGCCTCAT CCGCTGTCTC CTCCGCAGGG AGCCTTCAGA GCGACTTGTG 
GCCCTGGGAA TCCTGCTACA TCCCTGGTTG AGGGAGGATT GCAGCCAAGT CTCTCCTCCA 
CGGTCTGACC GAAGGGAGAT GGACCAGGTT GTCCCAGATG GGCCACAGCT GGAGGAGGCA 
GAGGAAGGGG AGGTGGGGCT GTATGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of TRB3 protects neurons against apoptosis induced by global cerebral ischemia and reperfusion injury in rats.
Neuroscience360118-127(2017 Sep)
Wei K,Wan L,Liu J,Zhang B,Li X,Zhang Y,Zhang C,Yao W

TRB3 mediates advanced glycation end product-induced apoptosis of pancreatic ?-cells through the protein kinase C ? pathway.
International journal of molecular medicine40(1)130-136(2017 Jul)
Wang M,Zhang W,Xu S,Peng L,Wang Z,Liu H,Fang Q,Deng T,Men X,Lou J

TRB3 gene silencing activates AMPK in adipose tissue with beneficial metabolic effects in obese and diabetic rats.
Biochemical and biophysical research communications488(1)22-28(2017 06)
Sun X,Song M,Wang H,Zhou H,Wang F,Li Y,Zhang Y,Zhang W,Zhong M,Ti Y

Silencing of TRB3 Ameliorates Diabetic Tubule Interstitial Nephropathy via PI3K/AKT Signaling in Rats.
Medical science monitor : international medical journal of experimental and clinical research232816-2824(2017 Jun)
Ma Y,Chen F,Yang S,Duan Y,Sun Z,Shi J

Nupr1 Modulates Methamphetamine-Induced Dopaminergic Neuronal Apoptosis and Autophagy through CHOP-Trib3-Mediated Endoplasmic Reticulum Stress Signaling Pathway.
Frontiers in molecular neuroscience10203(2017)
Xu X,Huang E,Tai Y,Zhao X,Chen X,Chen C,Chen R,Liu C,Lin Z,Wang H,Xie WB

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Trib3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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