Serpine1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Serpine1
Entrez Gene ID	24617
Full Name	serpin family E member 1
Synonyms	PAI1A,Pai1,Pai1aa,Planh,RATPAI1A
General protein information	Preferred Names serpin family E member 1 Names plasminogen activator inhibitor 1 PAI PAI-1 endothelial plasminogen activator inhibitor serine (or cysteine) peptidase inhibitor, clade E, member 1 serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 serine (or cysteine) proteinase inhibitor, clade E, member 1 serine (or cysteine) proteinase inhibitor, member 1 serpin E1 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q12
Summary	mediates inhibition of fibrinolysis by inhibiting the activity of plasminogen activator; may promote neuronal survival [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012620.1	NP_036752.1	plasminogen activator inhibitor 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SERPINE1	XP_527841.3
Bos taurus (cattle)	SERPINE1	NP_776562.1
	SERPINE1	XP_001107647.1
Canis lupus familiaris (dog)	SERPINE1	NP_001184024.1
Xenopus tropicalis (tropical clawed frog)	serpine1	XP_002941247.2
Mus musculus (house mouse)	Serpine1	NP_032897.2
Rattus norvegicus (Norway rat)	Serpine1	NP_036752.1
Anopheles gambiae (African malaria mosquito)	SRPN1	XP_308847.4
Anopheles gambiae (African malaria mosquito)	SRPN2	XP_308845.4
Homo sapiens (human)	SERPINE1	NP_000593.1
Danio rerio (zebrafish)	serpine1	NP_001108031.1
Anopheles gambiae (African malaria mosquito)	SRPN3	XP_308846.4

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-335-5p Suppresses Lower Extremity Deep Venous Thrombosis by Targeted Inhibition of PAI-1 via the TLR4 Signaling Pathway.
Bao CX, Zhang DX, Wang NN, Zhu XK, Zhao Q, Sun XL
Journal of cellular biochemistry(2017 Dec)

Serpine 1 induces alveolar type II cell senescence through activating p53-p21-Rb pathway in fibrotic lung disease.
Jiang C, Liu G, Luckhardt T, Antony V, Zhou Y, Carter AB, Thannickal VJ, Liu RM
Aging cell16(5)1114-1124(2017 Oct)

Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis.
Lee JG, Shim S, Kim MJ, Myung JK, Jang WS, Bae CH, Lee SJ, Kim KM, Jin YW, Lee SS, Park S
BioMed research international20171279280(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results demonstrate the antifibrotic effect of PTX on radiation-induced lung fibrosis and its effect on modulation of PKA and PAI-1 expression as possible antifibrotic mechanisms.
Title: Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis.

Paclitaxel at low non-cytotoxic doses ameliorates renal fibrosis by inhibiting multiple steps in the TGF-beta1-induced PAI-1 signaling including Smads and mitogen-activated protein kinases.
Title: Low-dose paclitaxel ameliorates renal fibrosis by suppressing transforming growth factor-?1-induced plasminogen activator inhibitor-1 signaling.

these results suggested that TGF-beta1 and Serpine 1 overexpression might play an important role in DVT formation and have predictive values.
Title: TGF-?1 and Serpine 1 expression changes in traumatic deep vein thrombosis.

Aldosterone at different concentrations exerts synergistic effect with TGF-beta1 to increase PAI-1 expression in hepatic stellate cells
Title: Aldosterone and TGF-?? synergistically increase PAI-1 expression in hepatic stellate cells of rats.

inhibition of ERK1/2 activation has no therapeutic effect on kidney fibrosis in SNx possibly due to increased compensatory activation of the p38 and JNK signalling pathways with subsequent upregulation of PAI-1.
Title: Inhibiting ERK Activation with CI-1040 Leads to Compensatory Upregulation of Alternate MAPKs and Plasminogen Activator Inhibitor-1 following Subtotal Nephrectomy with No Impact on Kidney Fibrosis.

The following Serpine1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Serpine1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12139	NM_012620.1 Latest version!	Rattus norvegicus serpin family E member 1 (Serpine1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Serpine1 ( NM_012620.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036752.1 Rattus norvegicus serpin family E member 1 (Serpine1), mRNA.

Price & Availability↑

CloneID	ORa12139
Clone ID Related Accession (Same CDS sequence)	NM_012620.1
Accession Version	NM_012620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	plasminogen activator inhibitor 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M24067.1. On Dec 17, 2003 this sequence version replaced XM_346431.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M24067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGCAGATGT CTTCAGCCCT CACTTGCCTC ACCCTGGGCC TGGTTCTGGT CTTTGGGAAA 
GGGTTCGCTT CACCCCTTCC AGAGTCCCAT ACAGCCCAGC AGGCCACCAA CTTCGGAGTA 
AAAGTGTTTC AGCATGTGGT CCAGGCCTCC AAAGACCGAA ATGTGGTCTT CTCTCCCTAC 
GGCGTGTCCT CGGTGCTGGC TATGCTGCAG CTGACCACAG CAGGGAAAAC CCGGCAGCAG 
ATCCAAGATG CTATGGGATT CAATATCAGT GAGAGGGGCA CAGCTCCTGC CCTCCGAAAG 
CTCTCCAAGG AGCTCATGGG GTCATGGAAC AAGAATGAGA TCAGTACTGC GGACGCCATC 
TTTGTCCAGC GGGACCTAGA GCTGGTCCAG GGCTTCATGC CCCACTTCTT CAAGCTCTTC 
CGGACCACGG TGAAGCAGGT GGACTTCTCA GAGGTGGAAA GAGCCAGATT CATCATCAAC 
GACTGGGTGG AGAGGCACAC CAAAGGTATG ATCAGTGACT TACTGGCCAA GGGGGCTGTA 
AATGAGCTGA CACGCCTGGT GCTGGTGAAC GCCCTCTATT TCAACGGCCA ATGGAAGACC 
CCCTTCTTAG AGGCCAGCAC CCACCAGCGC CTGTTCCACA AGTCTGATGG TAGCACCATC 
TCCGTGCCCA TGATGGCTCA GAACAACAAG TTCAACTACA CTGAGTTCAC CACTCCGGAT 
GGGCACGAGT ACGACATCCT GGAACTGCCC TACCACGGCG AAACCCTCAG CATGTTCATT 
GCAGCACCCT TTGAAAAAGA TGTGCCCCTC TCCGCCATCA CCAACATTTT GGACGCTGAG 
CTCATCAGAC AATGGAAGAG CAACATGACC AGGCTGCCCC GCCTCCTCAT CCTGCCTAAG 
TTCTCTCTGG AGACTGAAGT GGACCTCAGA GGGCCCCTGG AGAAGCTGGG CATGACTGAC 
ATCTTCAGCT CAACCCAGGC CGACTTCACA AGTCTTTCCG ACCAAGAGCA GCTCTCTGTA 
GCACAAGCAC TACAAAAGGT CAAGATCGAG GTGAACGAGA GCGGCACAGT GGCGTCTTCC 
TCCACAGCCA TTCTAGTCTC AGCCCGCATG GCCCCCACGG AGATGGTTTT AGACCGATCC 
TTTCTCTTTG TGGTTCGGCA CAATCCAACA GAGACAATCC TCTTCATGGG CCAGCTGATG 
GAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036752.1
CDS	119..1327
Translation	MQMSSALTCLTLGLVLVFGKGFASPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTETILFMGQLMEP

Target ORF information:

RefSeq Version	NM_012620.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serpin family E member 1 (Serpine1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012620.1

ORF Insert Sequence:

ATGCAGATGT CTTCAGCCCT CACTTGCCTC ACCCTGGGCC TGGTTCTGGT CTTTGGGAAA 
GGGTTCGCTT CACCCCTTCC AGAGTCCCAT ACAGCCCAGC AGGCCACCAA CTTCGGAGTA 
AAAGTGTTTC AGCATGTGGT CCAGGCCTCC AAAGACCGAA ATGTGGTCTT CTCTCCCTAC 
GGCGTGTCCT CGGTGCTGGC TATGCTGCAG CTGACCACAG CAGGGAAAAC CCGGCAGCAG 
ATCCAAGATG CTATGGGATT CAATATCAGT GAGAGGGGCA CAGCTCCTGC CCTCCGAAAG 
CTCTCCAAGG AGCTCATGGG GTCATGGAAC AAGAATGAGA TCAGTACTGC GGACGCCATC 
TTTGTCCAGC GGGACCTAGA GCTGGTCCAG GGCTTCATGC CCCACTTCTT CAAGCTCTTC 
CGGACCACGG TGAAGCAGGT GGACTTCTCA GAGGTGGAAA GAGCCAGATT CATCATCAAC 
GACTGGGTGG AGAGGCACAC CAAAGGTATG ATCAGTGACT TACTGGCCAA GGGGGCTGTA 
AATGAGCTGA CACGCCTGGT GCTGGTGAAC GCCCTCTATT TCAACGGCCA ATGGAAGACC 
CCCTTCTTAG AGGCCAGCAC CCACCAGCGC CTGTTCCACA AGTCTGATGG TAGCACCATC 
TCCGTGCCCA TGATGGCTCA GAACAACAAG TTCAACTACA CTGAGTTCAC CACTCCGGAT 
GGGCACGAGT ACGACATCCT GGAACTGCCC TACCACGGCG AAACCCTCAG CATGTTCATT 
GCAGCACCCT TTGAAAAAGA TGTGCCCCTC TCCGCCATCA CCAACATTTT GGACGCTGAG 
CTCATCAGAC AATGGAAGAG CAACATGACC AGGCTGCCCC GCCTCCTCAT CCTGCCTAAG 
TTCTCTCTGG AGACTGAAGT GGACCTCAGA GGGCCCCTGG AGAAGCTGGG CATGACTGAC 
ATCTTCAGCT CAACCCAGGC CGACTTCACA AGTCTTTCCG ACCAAGAGCA GCTCTCTGTA 
GCACAAGCAC TACAAAAGGT CAAGATCGAG GTGAACGAGA GCGGCACAGT GGCGTCTTCC 
TCCACAGCCA TTCTAGTCTC AGCCCGCATG GCCCCCACGG AGATGGTTTT AGACCGATCC 
TTTCTCTTTG TGGTTCGGCA CAATCCAACA GAGACAATCC TCTTCATGGG CCAGCTGATG 
GAGCCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-335-5p Suppresses Lower Extremity Deep Venous Thrombosis by Targeted Inhibition of PAI-1 via the TLR4 Signaling Pathway.
Journal of cellular biochemistry(2017 Dec)
Bao CX,Zhang DX,Wang NN,Zhu XK,Zhao Q,Sun XL

Serpine 1 induces alveolar type II cell senescence through activating p53-p21-Rb pathway in fibrotic lung disease.
Aging cell16(5)1114-1124(2017 Oct)
Jiang C,Liu G,Luckhardt T,Antony V,Zhou Y,Carter AB,Thannickal VJ,Liu RM

Pentoxifylline Regulates Plasminogen Activator Inhibitor-1 Expression and Protein Kinase A Phosphorylation in Radiation-Induced Lung Fibrosis.
BioMed research international20171279280(2017)
Lee JG,Shim S,Kim MJ,Myung JK,Jang WS,Bae CH,Lee SJ,Kim KM,Jin YW,Lee SS,Park S

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Serpine1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報