Me1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Me1
Entrez Gene ID	24552
Full Name	malic enzyme 1
Synonyms	MOD1
General protein information	Preferred Names malic enzyme 1 Names NADP-dependent malic enzyme NADP-ME cytosolic malic enzyme 1 malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) malic enzyme 1, NADP(+)-dependent, cytosolic malic enzyme 1, soluble
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q31
Summary	catalyzes the NADP+ dependent conversion of S-malate to pyruvate [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012600.2	NP_036732.2	NADP-dependent malic enzyme
XM_008766439.2	XP_008764661.1	NADP-dependent malic enzyme isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ME1	NP_002386.1
Pan troglodytes (chimpanzee)	ME1	XP_518610.3
Bos taurus (cattle)	ME1	NP_001138325.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000184	XP_310951.5
Neurospora crassa	NCU03651	XP_960731.2
Oryza sativa (rice)	Os05g0186300	NP_001054832.1
Canis lupus familiaris (dog)	ME1	XP_532217.4
Magnaporthe oryzae (rice blast fungus)	MGG_08173	XP_003715145.1
Arabidopsis thaliana (thale cress)	NADP-ME4	NP_178093.1
Drosophila melanogaster (fruit fly)	Men	NP_731739.1
Oryza sativa (rice)	Os01g0188400	NP_001042257.1
Mus musculus (house mouse)	Me1	NP_032641.2
Rattus norvegicus (Norway rat)	Me1	NP_036732.2
Gallus gallus (chicken)	ME1	NP_989634.1
Xenopus tropicalis (tropical clawed frog)	me1	XP_002938500.1

Gene Ontology
Bibliography

Related articles in PubMed

Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.
Heart E, Cline GW, Collis LP, Pongratz RL, Gray JP, Smith PJ
American journal of physiology. Endocrinology and metabolism296(6)E1354-62(2009 Jun)

Silencing of cytosolic or mitochondrial isoforms of malic enzyme has no effect on glucose-stimulated insulin secretion from rodent islets.
Ronnebaum SM, Jensen MV, Hohmeier HE, Burgess SC, Zhou YP, Qian S, MacNeil D, Howard A, Thornberry N, Ilkayeva O, Lu D, Sherry AD, Newgard CB
The Journal of biological chemistry283(43)28909-17(2008 Oct)

Dietary lipoic acid-dependent changes in the activity and mRNA levels of hepatic lipogenic enzymes in rats.
Huong DT, Ide T
The British journal of nutrition100(1)79-87(2008 Jul)

Effect of anabolic steroid nandrolone decanoate on the properties of certain enzymes in the heart, liver, and muscle of rats, and their effect on rats' cardiac electrophysiology.
Tylicki A, Kawalko A, Sokolska J, Strumilo S
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme39(4)268-72(2007 Apr)

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Kwitek AE, Gullings-Handley J, Yu J, Carlos DC, Orlebeke K, Nie J, Eckert J, Lemke A, Andrae JW, Bromberg S, Pasko D, Chen D, Scheetz TE, Casavant TL, Soares MB, Sheffield VC, Tonellato PJ, Jacob HJ
Genome research14(4)750-7(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

although ME1 overexpression augments anaplerosis and glucose stimulated insulin secretion in INS-1 832/13 cells, it is not likely involved in methyl succinate and glucose stimulated insulin secretion in pancreatic islets
Title: Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.

cytosolic or mitochondrial isoforms of malic enzyme do not affect glucose-stimulated insulin secretion from rodent islets
Title: Silencing of cytosolic or mitochondrial isoforms of malic enzyme has no effect on glucose-stimulated insulin secretion from rodent islets.

The following Me1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Me1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa39873	NM_012600.2 Latest version!	Rattus norvegicus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$335.30 $479.00
ORa42921	XM_008766439.2 Latest version!	Rattus norvegicus malic enzyme 1 (Me1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Me1 ( NM_012600.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036732.2 Rattus norvegicus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), mRNA.

Price & Availability↑

CloneID	ORa39873
Clone ID Related Accession (Same CDS sequence)	NM_012600.2
Accession Version	NM_012600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2012-04-28
Organism	Rattus norvegicus(Norway rat)
Product	NADP-dependent malic enzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR03064582.1, AABR03064643.1, CB810743.1, H34871.1, CO560778.1, CB613492.1, CO395247.1 and AABR03062333.1. On Oct 17, 2007 this sequence version replaced NM_012600.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGATCCCC GAGCCCCCCG ACGCCGACAC GCCCACCAGC GCGGCTACCT GCTGACGCGG 
GACCCGCATC TCAACAAGGA CTTGGCTTTT ACTCTGGAAG AGAGGCAACA GCTGAACATT 
CATGGCTTGT TGCCACCCTG CATTGTCAAC CAGGAGATCC AGGTCCTTAG AGTAATTAAG 
AATTTCGAGC GTCTGAACTC TGACTTCGAC AGGTATCTTC TGTTAATGGA TCTGCAAGAT 
AGGAATGAGA AGCTCTTCTA CAGTGTGCTT ATGTCTAATG TTGAAAAGTT CATGCCTATC 
GTTTACACTC CCACCGTGGG TCTTGCATGC CAACAATACA GTTTGGCATT CCGGAAGCCA 
AGAGGCCTCT TTATCAGTAT CCACGACAAA GGGCATATTG CTTCAGTTCT TAACGCATGG 
CCAGAAGATG TTGTCAAGGC TATTGTGGTG ACTGATGGAG AGCGAATCCT CGGCTTGGGC 
GACCTTGGTT GTAACGGGAT GGGCATCCCT GTGGGTAAAC TGGCCCTGTA CACAGCGTGC 
GGAGGGGTGA ATCCACAACA GTGTCTACCC ATCACTTTGG ACGTGGGCAC AGAAAATGAG 
GAGTTACTTA AAGATCCCTT GTATATTGGG CTGCGGCACA GGCGAGTGAG AGGCCCTGAA 
TATGATGCGT TTTTGGATGA ATTCATGGAG GCAGCGTCTT CCAAATATGG CATGAATTGC 
CTTATTCAGT TTGAAGATTT TGCCAATCTG AATGCATTTC GTCTCCTGAA CAAGTATCGA 
AACAAGTATT GCACATTTAA CGATGATATT CAAGGAACAG CGTCTGTGGC AGTTGCCGGC 
CTTCTTGCTG CTCTTCGGAT AACCAAGAAC AAGCTCTCTG ATCAGACAGT GCTGTTCCAG 
GGAGCCGGCG AGGCTGCCTT GGGGATTGCT CATCTGATTG TTATGGCCAT GGAGAAGGAA 
GGTTTATCAA AGGAGAAAGC TAGACAAAAG ATATGGTTGG TTGACTCAAA AGGATTAATA 
GTTAAGGGGC GTGCTTCTCT CACAGAAGAG AAAGAGGTGT TTGCCCATGA ACATGAAGAA 
ATGAAGAACC TAGAAGCCAT TGTTCAGAAG ATAAAACCAA CCGCTCTCAT AGGAGTTGCT 
GCAATTGGTG GTGCTTTCAC AGAACAAATT CTCAAGGATA TGGCTGCCTT CAACGAGCGG 
CCCATCATCT TTGCTTTGAG TAATCCGACC AGCAAAGCTG AGTGTTCTGC AGAGGAGTGC 
TATAAAGTGA CCAAGGGCCG TGCGATCTTT GCCAGCGGCA GTCCTTTTGA TCCAGTCACT 
CTTCCAGATG GACGGACTCT GTTTCCTGGC CAAGGCAACA ACTCCTATGT GTTCCCTGGA 
GTTGCTCTTG GGGTAGTGGC CTGTGGACTG AGACACATCA ATGATTCGGT CTTCCTCACC 
ACGGCTGAGG TCATATCCCA GCAAGTGTCA GATAAACACC TAGAAGAAGG CCGGCTCTAT 
CCTCCTTTGA ACACCATCCG AGATGTTTCC TTGAAAATCG CAGTAAAGAT TGTGCAAGAT 
GCATACAAAG AAAAGATGGC CACTGTTTAT CCTGAACCCC AAAACAAAGA AGAATTTGTC 
TCCTCCCAGA TGTACAGCAC TAATTATGAC CAGATCCTAC CTGATTGTTA TTCGTGGCCT 
GAAGAAGTCC AGAAAATACA GACCAAAGTC AATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036732.2
CDS	1..1719
Translation	MDPRAPRRRHAHQRGYLLTRDPHLNKDLAFTLEERQQLNIHGLLPPCIVNQEIQVLRVIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSNVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANLNAFRLLNKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEKEGLSKEKARQKIWLVDSKGLIVKGRASLTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEECYKVTKGRAIFASGSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHINDSVFLTTAEVISQQVSDKHLEEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYSWPEEVQKIQTKVNQ

Target ORF information:

RefSeq Version	NM_012600.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus malic enzyme 1, NADP(+)-dependent, cytosolic (Me1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012600.2

ORF Insert Sequence:

ATGGATCCCC GAGCCCCCCG ACGCCGACAC GCCCACCAGC GCGGCTACCT GCTGACGCGG 
GACCCGCATC TCAACAAGGA CTTGGCTTTT ACTCTGGAAG AGAGGCAACA GCTGAACATT 
CATGGCTTGT TGCCACCCTG CATTGTCAAC CAGGAGATCC AGGTCCTTAG AGTAATTAAG 
AATTTCGAGC GTCTGAACTC TGACTTCGAC AGGTATCTTC TGTTAATGGA TCTGCAAGAT 
AGGAATGAGA AGCTCTTCTA CAGTGTGCTT ATGTCTAATG TTGAAAAGTT CATGCCTATC 
GTTTACACTC CCACCGTGGG TCTTGCATGC CAACAATACA GTTTGGCATT CCGGAAGCCA 
AGAGGCCTCT TTATCAGTAT CCACGACAAA GGGCATATTG CTTCAGTTCT TAACGCATGG 
CCAGAAGATG TTGTCAAGGC TATTGTGGTG ACTGATGGAG AGCGAATCCT CGGCTTGGGC 
GACCTTGGTT GTAACGGGAT GGGCATCCCT GTGGGTAAAC TGGCCCTGTA CACAGCGTGC 
GGAGGGGTGA ATCCACAACA GTGTCTACCC ATCACTTTGG ACGTGGGCAC AGAAAATGAG 
GAGTTACTTA AAGATCCCTT GTATATTGGG CTGCGGCACA GGCGAGTGAG AGGCCCTGAA 
TATGATGCGT TTTTGGATGA ATTCATGGAG GCAGCGTCTT CCAAATATGG CATGAATTGC 
CTTATTCAGT TTGAAGATTT TGCCAATCTG AATGCATTTC GTCTCCTGAA CAAGTATCGA 
AACAAGTATT GCACATTTAA CGATGATATT CAAGGAACAG CGTCTGTGGC AGTTGCCGGC 
CTTCTTGCTG CTCTTCGGAT AACCAAGAAC AAGCTCTCTG ATCAGACAGT GCTGTTCCAG 
GGAGCCGGCG AGGCTGCCTT GGGGATTGCT CATCTGATTG TTATGGCCAT GGAGAAGGAA 
GGTTTATCAA AGGAGAAAGC TAGACAAAAG ATATGGTTGG TTGACTCAAA AGGATTAATA 
GTTAAGGGGC GTGCTTCTCT CACAGAAGAG AAAGAGGTGT TTGCCCATGA ACATGAAGAA 
ATGAAGAACC TAGAAGCCAT TGTTCAGAAG ATAAAACCAA CCGCTCTCAT AGGAGTTGCT 
GCAATTGGTG GTGCTTTCAC AGAACAAATT CTCAAGGATA TGGCTGCCTT CAACGAGCGG 
CCCATCATCT TTGCTTTGAG TAATCCGACC AGCAAAGCTG AGTGTTCTGC AGAGGAGTGC 
TATAAAGTGA CCAAGGGCCG TGCGATCTTT GCCAGCGGCA GTCCTTTTGA TCCAGTCACT 
CTTCCAGATG GACGGACTCT GTTTCCTGGC CAAGGCAACA ACTCCTATGT GTTCCCTGGA 
GTTGCTCTTG GGGTAGTGGC CTGTGGACTG AGACACATCA ATGATTCGGT CTTCCTCACC 
ACGGCTGAGG TCATATCCCA GCAAGTGTCA GATAAACACC TAGAAGAAGG CCGGCTCTAT 
CCTCCTTTGA ACACCATCCG AGATGTTTCC TTGAAAATCG CAGTAAAGAT TGTGCAAGAT 
GCATACAAAG AAAAGATGGC CACTGTTTAT CCTGAACCCC AAAACAAAGA AGAATTTGTC 
TCCTCCCAGA TGTACAGCAC TAATTATGAC CAGATCCTAC CTGATTGTTA TTCGTGGCCT 
GAAGAAGTCC AGAAAATACA GACCAAAGTC AATCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Me1 ( XM_008766439.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764661.1 Rattus norvegicus malic enzyme 1 (Me1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42921
Clone ID Related Accession (Same CDS sequence)	XM_008766439.2
Accession Version	XM_008766439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NADP-dependent malic enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTTGG CTTTTACTCT GGAAGAGAGG CAACAGCTGA ACATTCATGG CTTGTTGCCA 
CCCTGCATTG TCAACCAGGA GATCCAGGTC CTTAGAGTAA TTAAGAATTT CGAGCGTCTG 
AACTCTGACT TCGACAGGTA TCTTCTGTTA ATGGATCTGC AAGATAGGAA TGAGAAGCTC 
TTCTACAGTG TGCTTATGTC TAATGTTGAA AAGTTCATGC CTATCGTTTA CACTCCCACC 
GTGGGTCTTG CATGCCAACA ATACAGTTTG GCATTCCGGA AGCCAAGAGG CCTCTTTATC 
AGTATCCACG ACAAAGGGCA TATTGCTTCA GTTCTTAACG CATGGCCAGA AGATGTTGTC 
AAGGCTATTG TGGTGACTGA TGGAGAGCGA ATCCTCGGCT TGGGCGACCT TGGTTGTAAC 
GGGATGGGCA TCCCTGTGGG TAAACTGGCC CTGTACACAG CGTGCGGAGG GGTGAATCCA 
CAACAGTGTC TACCCATCAC TTTGGACGTG GGCACAGAAA ATGAGGAGTT ACTTAAAGAT 
CCCTTGTATA TTGGGCTGCG GCACAGGCGA GTGAGAGGCC CTGAATATGA TGCGTTTTTG 
GATGAATTCA TGGAGGCAGC GTCTTCCAAA TATGGCATGA ATTGCCTTAT TCAGTTTGAA 
GATTTTGCCA ATCTGAATGC ATTTCGTCTC CTGAACAAGT ATCGAAACAA GTATTGCACA 
TTTAACGATG ATATTCAAGG AACAGCGTCT GTGGCAGTTG CCGGCCTTCT TGCTGCTCTT 
CGGATAACCA AGAACAAGCT CTCTGATCAG ACAGTGCTGT TCCAGGGAGC CGGCGAGGCT 
GCCTTGGGGA TTGCTCATCT GATTGTTATG GCCATGGAGA AGGAAGGTTT ATCAAAGGAG 
AAAGCTAGAC AAAAGATATG GTTGGTTGAC TCAAAAGGAT TAATAGTTAA GGGGCGTGCT 
TCTCTCACAG AAGAGAAAGA GGTGTTTGCC CATGAACATG AAGAAATGAA GAACCTAGAA 
GCCATTGTTC AGAAGATAAA ACCAACCGCT CTCATAGGAG TTGCTGCAAT TGGTGGTGCT 
TTCACAGAAC AAATTCTCAA GGATATGGCT GCCTTCAACG AGCGGCCCAT CATCTTTGCT 
TTGAGTAATC CGACCAGCAA AGCTGAGTGT TCTGCAGAGG AGTGCTATAA AGTGACCAAG 
GGCCGTGCGA TCTTTGCCAG CGGCAGTCCT TTTGATCCAG TCACTCTTCC AGATGGACGG 
ACTCTGTTTC CTGGCCAAGG CAACAACTCC TATGTGTTCC CTGGAGTTGC TCTTGGGGTA 
GTGGCCTGTG GACTGAGACA CATCAATGAT TCGGTCTTCC TCACCACGGC TGAGGTCATA 
TCCCAGCAAG TGTCAGATAA ACACCTAGAA GAAGGCCGGC TCTATCCTCC TTTGAACACC 
ATCCGAGATG TTTCCTTGAA AATCGCAGTA AAGATTGTGC AAGATGCATA CAAAGAAAAG 
ATGGCCACTG TTTATCCTGA ACCCCAAAAC AAAGAAGAAT TTGTCTCCTC CCAGATGTAC 
AGCACTAATT ATGACCAGAT CCTACCTGAT TGTTATTCGT GGCCTGAAGA AGTCCAGAAA 
ATACAGACCA AAGTCAATCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764661.1
CDS	358..2001
Translation	MDLAFTLEERQQLNIHGLLPPCIVNQEIQVLRVIKNFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSNVEKFMPIVYTPTVGLACQQYSLAFRKPRGLFISIHDKGHIASVLNAWPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTENEELLKDPLYIGLRHRRVRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANLNAFRLLNKYRNKYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTVLFQGAGEAALGIAHLIVMAMEKEGLSKEKARQKIWLVDSKGLIVKGRASLTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAIGGAFTEQILKDMAAFNERPIIFALSNPTSKAECSAEECYKVTKGRAIFASGSPFDPVTLPDGRTLFPGQGNNSYVFPGVALGVVACGLRHINDSVFLTTAEVISQQVSDKHLEEGRLYPPLNTIRDVSLKIAVKIVQDAYKEKMATVYPEPQNKEEFVSSQMYSTNYDQILPDCYSWPEEVQKIQTKVNQ

Target ORF information:

RefSeq Version	XM_008766439.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus malic enzyme 1 (Me1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766439.2

ORF Insert Sequence:

ATGGACTTGG CTTTTACTCT GGAAGAGAGG CAACAGCTGA ACATTCATGG CTTGTTGCCA 
CCCTGCATTG TCAACCAGGA GATCCAGGTC CTTAGAGTAA TTAAGAATTT CGAGCGTCTG 
AACTCTGACT TCGACAGGTA TCTTCTGTTA ATGGATCTGC AAGATAGGAA TGAGAAGCTC 
TTCTACAGTG TGCTTATGTC TAATGTTGAA AAGTTCATGC CTATCGTTTA CACTCCCACC 
GTGGGTCTTG CATGCCAACA ATACAGTTTG GCATTCCGGA AGCCAAGAGG CCTCTTTATC 
AGTATCCACG ACAAAGGGCA TATTGCTTCA GTTCTTAACG CATGGCCAGA AGATGTTGTC 
AAGGCTATTG TGGTGACTGA TGGAGAGCGA ATCCTCGGCT TGGGCGACCT TGGTTGTAAC 
GGGATGGGCA TCCCTGTGGG TAAACTGGCC CTGTACACAG CGTGCGGAGG GGTGAATCCA 
CAACAGTGTC TACCCATCAC TTTGGACGTG GGCACAGAAA ATGAGGAGTT ACTTAAAGAT 
CCCTTGTATA TTGGGCTGCG GCACAGGCGA GTGAGAGGCC CTGAATATGA TGCGTTTTTG 
GATGAATTCA TGGAGGCAGC GTCTTCCAAA TATGGCATGA ATTGCCTTAT TCAGTTTGAA 
GATTTTGCCA ATCTGAATGC ATTTCGTCTC CTGAACAAGT ATCGAAACAA GTATTGCACA 
TTTAACGATG ATATTCAAGG AACAGCGTCT GTGGCAGTTG CCGGCCTTCT TGCTGCTCTT 
CGGATAACCA AGAACAAGCT CTCTGATCAG ACAGTGCTGT TCCAGGGAGC CGGCGAGGCT 
GCCTTGGGGA TTGCTCATCT GATTGTTATG GCCATGGAGA AGGAAGGTTT ATCAAAGGAG 
AAAGCTAGAC AAAAGATATG GTTGGTTGAC TCAAAAGGAT TAATAGTTAA GGGGCGTGCT 
TCTCTCACAG AAGAGAAAGA GGTGTTTGCC CATGAACATG AAGAAATGAA GAACCTAGAA 
GCCATTGTTC AGAAGATAAA ACCAACCGCT CTCATAGGAG TTGCTGCAAT TGGTGGTGCT 
TTCACAGAAC AAATTCTCAA GGATATGGCT GCCTTCAACG AGCGGCCCAT CATCTTTGCT 
TTGAGTAATC CGACCAGCAA AGCTGAGTGT TCTGCAGAGG AGTGCTATAA AGTGACCAAG 
GGCCGTGCGA TCTTTGCCAG CGGCAGTCCT TTTGATCCAG TCACTCTTCC AGATGGACGG 
ACTCTGTTTC CTGGCCAAGG CAACAACTCC TATGTGTTCC CTGGAGTTGC TCTTGGGGTA 
GTGGCCTGTG GACTGAGACA CATCAATGAT TCGGTCTTCC TCACCACGGC TGAGGTCATA 
TCCCAGCAAG TGTCAGATAA ACACCTAGAA GAAGGCCGGC TCTATCCTCC TTTGAACACC 
ATCCGAGATG TTTCCTTGAA AATCGCAGTA AAGATTGTGC AAGATGCATA CAAAGAAAAG 
ATGGCCACTG TTTATCCTGA ACCCCAAAAC AAAGAAGAAT TTGTCTCCTC CCAGATGTAC 
AGCACTAATT ATGACCAGAT CCTACCTGAT TGTTATTCGT GGCCTGAAGA AGTCCAGAAA 
ATACAGACCA AAGTCAATCA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion.
American journal of physiology. Endocrinology and metabolism296(6)E1354-62(2009 Jun)
Heart E,Cline GW,Collis LP,Pongratz RL,Gray JP,Smith PJ

Silencing of cytosolic or mitochondrial isoforms of malic enzyme has no effect on glucose-stimulated insulin secretion from rodent islets.
The Journal of biological chemistry283(43)28909-17(2008 Oct)
Ronnebaum SM,Jensen MV,Hohmeier HE,Burgess SC,Zhou YP,Qian S,MacNeil D,Howard A,Thornberry N,Ilkayeva O,Lu D,Sherry AD,Newgard CB

Dietary lipoic acid-dependent changes in the activity and mRNA levels of hepatic lipogenic enzymes in rats.
The British journal of nutrition100(1)79-87(2008 Jul)
Huong DT,Ide T

Effect of anabolic steroid nandrolone decanoate on the properties of certain enzymes in the heart, liver, and muscle of rats, and their effect on rats' cardiac electrophysiology.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme39(4)268-72(2007 Apr)
Tylicki A,Kawalko A,Sokolska J,Strumilo S

High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence.
Genome research14(4)750-7(2004 Apr)
Kwitek AE,Gullings-Handley J,Yu J,Carlos DC,Orlebeke K,Nie J,Eckert J,Lemke A,Andrae JW,Bromberg S,Pasko D,Chen D,Scheetz TE,Casavant TL,Soares MB,Sheffield VC,Tonellato PJ,Jacob HJ

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Me1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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