Ldha cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ldha
Entrez Gene ID	24533
Full Name	lactate dehydrogenase A
Synonyms	CDK1,Ldh1
General protein information	Preferred Names lactate dehydrogenase A Names L-lactate dehydrogenase A chain LDH muscle subunit LDH-A LDH-M lactate dehydrogenase A-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22
Summary	mRNA expression in fibroblasts increases in response to induction by epidermal growth factor or serum [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229232.3	XP_006229294.1	L-lactate dehydrogenase A chain isoform X1
NM_017025.1	NP_058721.1	L-lactate dehydrogenase A chain

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ldha	NP_058721.1
Rattus norvegicus (Norway rat)	RGD1562690	XP_003750506.1

Gene Ontology
Bibliography

Related articles in PubMed

Attenuation of
Zhang Y, Liu G, Gao X
Bioscience reports37(6)(2017 Dec)

Lactate dehydrogenase-A is indispensable for vascular smooth muscle cell proliferation and migration.
Kim JH, Bae KH, Byun JK, Lee S, Kim JG, Lee IK, Jung GS, Lee YM, Park KG
Biochemical and biophysical research communications492(1)41-47(2017 10)

Activation of HIF-1? and its downstream targets in rat hippocampus after long-term simulated microgravity exposure.
Wang T, Chen H, Lv K, Ji G, Liang F, Zhang Y, Wang Y, Liu X, Cao H, Kan G, Xiong J, Li Y, Qu L
Biochemical and biophysical research communications485(3)591-597(2017 Apr)

In situ lactate dehydrogenase activity: a novel renal cortical imaging biomarker of tubular injury?
Nielsen PM, Laustsen C, Bertelsen LB, Qi H, Mikkelsen E, Kristensen ML, N?rregaard R, St?dkilde-J?rgensen H
American journal of physiology. Renal physiology312(3)F465-F473(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

role of lactate dehydrogenase-A (LDHA), which is a key enzyme for glycolysis, in the proliferation and migration of vascular smooth muscle cells
Title: Lactate dehydrogenase-A is indispensable for vascular smooth muscle cell proliferation and migration.

The expression of three well-defined downstream targets of hypoxia inducible factor 1 alpha subunit (HIF-1alpha), pyruvate dehydrogenase kinase 1 (PDK1), lactate dehydrogenase A (LDHA) and vascular endothelial growth factor (VEGF), were up-regulated in hippocampus after 28 days of simulated microgravity exposure.
Title: Activation of HIF-1? and its downstream targets in rat hippocampus after long-term simulated microgravity exposure.

After 30 min of ischemia, the kidney maintains LDH metabolic function in spite of decreased kidney function, whereas the postischemic kidney after 60 min of ischemia show a generally reduced LDH enzyme activity concomitant with a reduced kidney function.
Title: In situ lactate dehydrogenase activity: a novel renal cortical imaging biomarker of tubular injury?

aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration
Title: Lactate dehydrogenase regulation in aged skeletal muscle: Regulation by anabolic steroids and functional overload.

Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium.
Title: Lactate dehydrogenase a expression is necessary to sustain rapid angiogenesis of pulmonary microvascular endothelium.

The following Ldha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ldha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02553	NM_017025.1 Latest version!	Rattus norvegicus lactate dehydrogenase A (Ldha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa22808	XM_006229232.3 Latest version!	Rattus norvegicus lactate dehydrogenase A (Ldha), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ldha ( NM_017025.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058721.1 Rattus norvegicus lactate dehydrogenase A (Ldha), mRNA.

Price & Availability↑

CloneID	ORa02553
Clone ID Related Accession (Same CDS sequence)	NM_017025.1
Accession Version	NM_017025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	L-lactate dehydrogenase A chain
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X01964.1. On Jun 25, 2006 this sequence version replaced XM_001080828.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X01964.1, CF110928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCCC TCAAGGACCA GCTGATTGTG AATCTTCTTA AGGAAGAACA GGTCCCCCAG 
AACAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CTTGTGCCAT CAGTATCTTA 
ATGAAGGACT TGGCTGATGA GCTTGCCCTT GTTGATGTCA TAGAAGATAA GCTAAAGGGA 
GAGATGATGG ATCTTCAGCA TGGCAGCCTT TTCCTTAAGA CACCAAAAAT TGTCTCCAGC 
AAAGATTATA GTGTGACTGC AAACTCCAAG CTGGTCATTA TCACCGCGGG GGCCCGTCAG 
CAAGAGGGAG AGAGCCGGCT CAATTTGGTC CAGCGAAACG TGAACATCTT CAAGTTCATC 
ATTCCAAATG TTGTGAAATA CAGTCCACAG TGCAAACTGC TCATCGTCTC AAACCCAGTG 
GATATCTTGA CCTACGTGGC TTGGAAGATC AGCGGCTTCC CCAAAAACAG AGTTATTGGA 
AGTGGTTGCA ATCTGGATTC GGCTCGGTTC CGTTACCTGA TGGGAGAAAG GCTGGGAGTT 
CATCCACTGA GCTGTCACGG GTGGGTCCTG GGAGAGCATG GCGACTCCAG TGTGCCTGTG 
TGGAGTGGTG TGAACGTCGC CGGCGTCTCC CTGAAGTCTC TGAACCCGCA GCTGGGCACG 
GATGCAGACA AGGAGCAGTG GAAGGATGTG CACAAGCAGG TGGTTGACAG TGCATACGAA 
GTGATCAAGC TGAAAGGTTA CACATCCTGG GCCATTGGCC TCTCCGTGGC AGACTTGGCC 
GAGAGCATAA TGAAGAACCT TAGGCGGGTG CATCCCATTT CCACCATGAT TAAGGGTCTC 
TATGGAATCA AGGAGGATGT CTTCCTCAGC GTCCCATGTA TCCTGGGACA AAATGGAATC 
TCAGATGTTG TGAAGGTGAC ACTGACTCCT GACGAGGAGG CCCGCCTGAA GAAGAGTGCA 
GATACCCTCT GGGGAATCCA GAAGGAGCTG CAGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058721.1
CDS	104..1102
Translation	MAALKDQLIVNLLKEEQVPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVSSKDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGVNVAGVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPISTMIKGLYGIKEDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKSADTLWGIQKELQF

Target ORF information:

RefSeq Version	NM_017025.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lactate dehydrogenase A (Ldha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017025.1

ORF Insert Sequence:

ATGGCAGCCC TCAAGGACCA GCTGATTGTG AATCTTCTTA AGGAAGAACA GGTCCCCCAG 
AACAAGATTA CAGTTGTTGG GGTTGGTGCT GTTGGCATGG CTTGTGCCAT CAGTATCTTA 
ATGAAGGACT TGGCTGATGA GCTTGCCCTT GTTGATGTCA TAGAAGATAA GCTAAAGGGA 
GAGATGATGG ATCTTCAGCA TGGCAGCCTT TTCCTTAAGA CACCAAAAAT TGTCTCCAGC 
AAAGATTATA GTGTGACTGC AAACTCCAAG CTGGTCATTA TCACCGCGGG GGCCCGTCAG 
CAAGAGGGAG AGAGCCGGCT CAATTTGGTC CAGCGAAACG TGAACATCTT CAAGTTCATC 
ATTCCAAATG TTGTGAAATA CAGTCCACAG TGCAAACTGC TCATCGTCTC AAACCCAGTG 
GATATCTTGA CCTACGTGGC TTGGAAGATC AGCGGCTTCC CCAAAAACAG AGTTATTGGA 
AGTGGTTGCA ATCTGGATTC GGCTCGGTTC CGTTACCTGA TGGGAGAAAG GCTGGGAGTT 
CATCCACTGA GCTGTCACGG GTGGGTCCTG GGAGAGCATG GCGACTCCAG TGTGCCTGTG 
TGGAGTGGTG TGAACGTCGC CGGCGTCTCC CTGAAGTCTC TGAACCCGCA GCTGGGCACG 
GATGCAGACA AGGAGCAGTG GAAGGATGTG CACAAGCAGG TGGTTGACAG TGCATACGAA 
GTGATCAAGC TGAAAGGTTA CACATCCTGG GCCATTGGCC TCTCCGTGGC AGACTTGGCC 
GAGAGCATAA TGAAGAACCT TAGGCGGGTG CATCCCATTT CCACCATGAT TAAGGGTCTC 
TATGGAATCA AGGAGGATGT CTTCCTCAGC GTCCCATGTA TCCTGGGACA AAATGGAATC 
TCAGATGTTG TGAAGGTGAC ACTGACTCCT GACGAGGAGG CCCGCCTGAA GAAGAGTGCA 
GATACCCTCT GGGGAATCCA GAAGGAGCTG CAGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ldha ( XM_006229232.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229294.1 Rattus norvegicus lactate dehydrogenase A (Ldha), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22808
Clone ID Related Accession (Same CDS sequence)	XM_006229232.3
Accession Version	XM_006229232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	L-lactate dehydrogenase A chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAGA ACTCAGGCGG CTACACATAT ACGGAGACCT CAGTATTATT TTTCCATTTC 
AAGGTCCCAA AAGATTCAAA GTCCAAGATG GCAGCCCTCA AGGACCAGCT GATTGTGAAT 
CTTCTTAAGG AAGAACAGGT CCCCCAGAAC AAGATTACAG TTGTTGGGGT TGGTGCTGTT 
GGCATGGCTT GTGCCATCAG TATCTTAATG AAGGACTTGG CTGATGAGCT TGCCCTTGTT 
GATGTCATAG AAGATAAGCT AAAGGGAGAG ATGATGGATC TTCAGCATGG CAGCCTTTTC 
CTTAAGACAC CAAAAATTGT CTCCAGCAAA GATTATAGTG TGACTGCAAA CTCCAAGCTG 
GTCATTATCA CCGCGGGGGC CCGTCAGCAA GAGGGAGAGA GCCGGCTCAA TTTGGTCCAG 
CGAAACGTGA ACATCTTCAA GTTCATCATT CCAAATGTTG TGAAATACAG TCCACAGTGC 
AAACTGCTCA TCGTCTCAAA CCCAGTGGAT ATCTTGACCT ACGTGGCTTG GAAGATCAGC 
GGCTTCCCCA AAAACAGAGT TATTGGAAGT GGTTGCAATC TGGATTCGGC TCGGTTCCGT 
TACCTGATGG GAGAAAGGCT GGGAGTTCAT CCACTGAGCT GTCACGGGTG GGTCCTGGGA 
GAGCATGGCG ACTCCAGTGT GCCTGTGTGG AGTGGTGTGA ATGTCGCCGG CGTCTCCCTG 
AAGTCTCTGA ACCCGCAGCT GGGCACGGAT GCAGACAAGG AGCAGTGGAA GGATGTGCAC 
AAGCAGGTGG TTGACAGTGC ATACGAAGTG ATCAAGCTGA AAGGTTACAC ATCCTGGGCC 
ATTGGCCTCT CCGTGGCAGA CTTGGCCGAG AGCATAATGA AGAACCTTAG GCGGGTGCAT 
CCCATTTCCA CCATGATTAA GGGTCTCTAT GGAATCAAGG AGGATGTCTT CCTCAGCGTC 
CCATGTATCC TGGGACAAAA TGGAATCTCA GATGTTGTGA AGGTGACACT GACTCCTGAC 
GAGGAGGCCC GCCTGAAGAA GAGTGCAGAT ACCCTCTGGG GAATCCAGAA GGAGCTGCAG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229294.1
CDS	181..1266
Translation	MSKNSGGYTYTETSVLFFHFKVPKDSKSKMAALKDQLIVNLLKEEQVPQNKITVVGVGAVGMACAISILMKDLADELALVDVIEDKLKGEMMDLQHGSLFLKTPKIVSSKDYSVTANSKLVIITAGARQQEGESRLNLVQRNVNIFKFIIPNVVKYSPQCKLLIVSNPVDILTYVAWKISGFPKNRVIGSGCNLDSARFRYLMGERLGVHPLSCHGWVLGEHGDSSVPVWSGVNVAGVSLKSLNPQLGTDADKEQWKDVHKQVVDSAYEVIKLKGYTSWAIGLSVADLAESIMKNLRRVHPISTMIKGLYGIKEDVFLSVPCILGQNGISDVVKVTLTPDEEARLKKSADTLWGIQKELQF

Target ORF information:

RefSeq Version	XM_006229232.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lactate dehydrogenase A (Ldha), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229232.3

ORF Insert Sequence:

ATGAGTAAGA ACTCAGGCGG CTACACATAT ACGGAGACCT CAGTATTATT TTTCCATTTC 
AAGGTCCCAA AAGATTCAAA GTCCAAGATG GCAGCCCTCA AGGACCAGCT GATTGTGAAT 
CTTCTTAAGG AAGAACAGGT CCCCCAGAAC AAGATTACAG TTGTTGGGGT TGGTGCTGTT 
GGCATGGCTT GTGCCATCAG TATCTTAATG AAGGACTTGG CTGATGAGCT TGCCCTTGTT 
GATGTCATAG AAGATAAGCT AAAGGGAGAG ATGATGGATC TTCAGCATGG CAGCCTTTTC 
CTTAAGACAC CAAAAATTGT CTCCAGCAAA GATTATAGTG TGACTGCAAA CTCCAAGCTG 
GTCATTATCA CCGCGGGGGC CCGTCAGCAA GAGGGAGAGA GCCGGCTCAA TTTGGTCCAG 
CGAAACGTGA ACATCTTCAA GTTCATCATT CCAAATGTTG TGAAATACAG TCCACAGTGC 
AAACTGCTCA TCGTCTCAAA CCCAGTGGAT ATCTTGACCT ACGTGGCTTG GAAGATCAGC 
GGCTTCCCCA AAAACAGAGT TATTGGAAGT GGTTGCAATC TGGATTCGGC TCGGTTCCGT 
TACCTGATGG GAGAAAGGCT GGGAGTTCAT CCACTGAGCT GTCACGGGTG GGTCCTGGGA 
GAGCATGGCG ACTCCAGTGT GCCTGTGTGG AGTGGTGTGA ATGTCGCCGG CGTCTCCCTG 
AAGTCTCTGA ACCCGCAGCT GGGCACGGAT GCAGACAAGG AGCAGTGGAA GGATGTGCAC 
AAGCAGGTGG TTGACAGTGC ATACGAAGTG ATCAAGCTGA AAGGTTACAC ATCCTGGGCC 
ATTGGCCTCT CCGTGGCAGA CTTGGCCGAG AGCATAATGA AGAACCTTAG GCGGGTGCAT 
CCCATTTCCA CCATGATTAA GGGTCTCTAT GGAATCAAGG AGGATGTCTT CCTCAGCGTC 
CCATGTATCC TGGGACAAAA TGGAATCTCA GATGTTGTGA AGGTGACACT GACTCCTGAC 
GAGGAGGCCC GCCTGAAGAA GAGTGCAGAT ACCCTCTGGG GAATCCAGAA GGAGCTGCAG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Attenuation of
Bioscience reports37(6)(2017 Dec)
Zhang Y,Liu G,Gao X

Lactate dehydrogenase-A is indispensable for vascular smooth muscle cell proliferation and migration.
Biochemical and biophysical research communications492(1)41-47(2017 10)
Kim JH,Bae KH,Byun JK,Lee S,Kim JG,Lee IK,Jung GS,Lee YM,Park KG

Activation of HIF-1? and its downstream targets in rat hippocampus after long-term simulated microgravity exposure.
Biochemical and biophysical research communications485(3)591-597(2017 Apr)
Wang T,Chen H,Lv K,Ji G,Liang F,Zhang Y,Wang Y,Liu X,Cao H,Kan G,Xiong J,Li Y,Qu L

In situ lactate dehydrogenase activity: a novel renal cortical imaging biomarker of tubular injury?
American journal of physiology. Renal physiology312(3)F465-F473(2017 Mar)
Nielsen PM,Laustsen C,Bertelsen LB,Qi H,Mikkelsen E,Kristensen ML,N?rregaard R,St?dkilde-J?rgensen H

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Ldha cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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