Ins2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ins2
Entrez Gene ID	24506
Full Name	insulin 2
Synonyms	CP-II
General protein information	Preferred Names insulin 2 Names insulin-2 C-peptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q41
Summary	gene encodes preproinsulin which is prossesed to form hormone involved in glucose and lipid metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019130.2	NP_062003.1	insulin-2 preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	INS	NP_001008996.1
Mus musculus (house mouse)	Ins2	NP_032413.1
Gallus gallus (chicken)	INS	NP_990553.1
Danio rerio (zebrafish)	ins	NP_571131.1
Xenopus tropicalis (tropical clawed frog)	ins	NP_001093706.1
Rattus norvegicus (Norway rat)	Ins2	NP_062003.1
Canis lupus familiaris (dog)	INS	NP_001123565.1
Homo sapiens (human)	INS	NP_000198.1

Gene Ontology
Bibliography

Related articles in PubMed

Ghrelin doesn't limit insulin release in pregnant rats fed low protein diet.
Gao H, Ho E, Yallampalli C
Frontiers in bioscience (Landmark edition)221523-1533(2017 06)

The combination of serum insulin, osteopontin, and hepatocyte growth factor predicts time to castration-resistant progression in androgen dependent metastatic prostate cancer- an exploratory study.
Dayyani F, Zurita AJ, Nogueras-Gonz?lez GM, Slack R, Millikan RE, Araujo JC, Gallick GE, Logothetis CJ, Corn PG
BMC cancer16721(2016 09)

Quercetin Stimulates Insulin Secretion and Reduces the Viability of Rat INS-1 Beta-Cells.
Kittl M, Beyreis M, Tumurkhuu M, F?rst J, Helm K, Pitschmann A, Gaisberger M, Glasl S, Ritter M, Jakab M
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(1)278-93(2016)

Rosuvastatin Treatment Affects Both Basal and Glucose-Induced Insulin Secretion in INS-1 832/13 Cells.
Salunkhe VA, Elvstam O, Eliasson L, Wendt A
PloS one11(3)e0151592(2016)

A variant insulin promoter in non-insulin-dependent diabetes mellitus.
Olansky L, Welling C, Giddings S, Adler S, Bourey R, Dowse G, Serjeantson S, Zimmet P, Permutt MA
The Journal of clinical investigation89(5)1596-602(1992 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of ghrelin and ghrelin receptor and glucose stimulated insulin secretion (GSIS) of pancreatic islets.
Title: Ghrelin doesn't limit insulin release in pregnant rats fed low protein diet.

Data show that quercetin acutely stimulates insulin release, inhibits cell proliferation and induces apoptosis of INS-1 beta cells.
Title: Quercetin Stimulates Insulin Secretion and Reduces the Viability of Rat INS-1 Beta-Cells.

Data suggest that rosuvastatin has the potential to increase basal insulin secretion and reduce glucose-induced insulin secretion.
Title: Rosuvastatin Treatment Affects Both Basal and Glucose-Induced Insulin Secretion in INS-1 832/13 Cells.

Data indicate that miR-183 was decreased and Foxo1 was upregulated during conversion of dental pulp cells (DPCs) to insulin-producing cells.
Title: Regulation of miRNA during direct reprogramming of dental pulp cells to insulin-producing cells.

Beta-cell proliferation increased in animals on tacrolimus while Ins2 gene expression remained unaltered.
Title: The higher diabetogenic risk of tacrolimus depends on pre-existing insulin resistance. A study in obese and lean Zucker rats.

The following Ins2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ins2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13254	NM_019130.2 Latest version!	Rattus norvegicus insulin 2 (Ins2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ins2 ( NM_019130.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062003.1 Rattus norvegicus insulin 2 (Ins2), mRNA.

Price & Availability↑

CloneID	ORa13254
Clone ID Related Accession (Same CDS sequence)	NM_019130.2
Accession Version	NM_019130.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	insulin-2 preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP467629.1, BP485158.1 and BP472188.1. On Jun 3, 2010 this sequence version replaced NM_019130.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP471674.1, BP485158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATCCGCTT CCTGCCCCTG CTGGCCCTGC TCATCCTCTG GGAGCCCCGC 
CCTGCCCAGG CTTTTGTCAA ACAGCACCTT TGTGGTTCTC ACTTGGTGGA AGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG ATTCTTCTAC ACACCCATGT CCCGCCGCGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGGG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCGGCA GAAGCGCGGC ATCGTGGATC AGTGCTGCAC CAGCATCTGC 
TCTCTCTACC AACTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062003.1
CDS	58..390
Translation	MALWIRFLPLLALLILWEPRPAQAFVKQHLCGSHLVEALYLVCGERGFFYTPMSRREVEDPQVAQLELGGGPGAGDLQTLALEVARQKRGIVDQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_019130.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus insulin 2 (Ins2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019130.2

ORF Insert Sequence:

ATGGCCCTGT GGATCCGCTT CCTGCCCCTG CTGGCCCTGC TCATCCTCTG GGAGCCCCGC 
CCTGCCCAGG CTTTTGTCAA ACAGCACCTT TGTGGTTCTC ACTTGGTGGA AGCTCTCTAC 
CTGGTGTGTG GGGAGCGTGG ATTCTTCTAC ACACCCATGT CCCGCCGCGA AGTGGAGGAC 
CCACAAGTGG CACAACTGGA GCTGGGTGGA GGCCCGGGGG CAGGTGACCT TCAGACCTTG 
GCACTGGAGG TGGCCCGGCA GAAGCGCGGC ATCGTGGATC AGTGCTGCAC CAGCATCTGC 
TCTCTCTACC AACTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ghrelin doesn't limit insulin release in pregnant rats fed low protein diet.
Frontiers in bioscience (Landmark edition)221523-1533(2017 06)
Gao H,Ho E,Yallampalli C

The combination of serum insulin, osteopontin, and hepatocyte growth factor predicts time to castration-resistant progression in androgen dependent metastatic prostate cancer- an exploratory study.
BMC cancer16721(2016 09)
Dayyani F,Zurita AJ,Nogueras-Gonz?lez GM,Slack R,Millikan RE,Araujo JC,Gallick GE,Logothetis CJ,Corn PG

Quercetin Stimulates Insulin Secretion and Reduces the Viability of Rat INS-1 Beta-Cells.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(1)278-93(2016)
Kittl M,Beyreis M,Tumurkhuu M,F?rst J,Helm K,Pitschmann A,Gaisberger M,Glasl S,Ritter M,Jakab M

Rosuvastatin Treatment Affects Both Basal and Glucose-Induced Insulin Secretion in INS-1 832/13 Cells.
PloS one11(3)e0151592(2016)
Salunkhe VA,Elvstam O,Eliasson L,Wendt A

A variant insulin promoter in non-insulin-dependent diabetes mellitus.
The Journal of clinical investigation89(5)1596-602(1992 May)
Olansky L,Welling C,Giddings S,Adler S,Bourey R,Dowse G,Serjeantson S,Zimmet P,Permutt MA

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Ins2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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