Gspt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gspt1
Entrez Gene ID	24420
Full Name	G1 to S phase transition 1
General protein information	Preferred Names G1 to S phase transition 1 Names eukaryotic peptide chain release factor GTP-binding subunit ERF3A G1 to S phase transition protein 1 G1 to phase transition 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q11
Summary	human homolog plays a role in translational elongation and in regulation of apoptosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596996.1	XP_017452485.1	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform X1
NM_001003978.1	NP_001003978.1	eukaryotic peptide chain release factor GTP-binding subunit ERF3A

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GSPT1	XP_001140542.3
Rattus norvegicus (Norway rat)	Gspt1	NP_001003978.1
Gallus gallus (chicken)	GSPT1	NP_001129149.1
Saccharomyces cerevisiae (baker's yeast)	SUP35	NP_010457.3
Homo sapiens (human)	GSPT1	NP_001123478.1
Danio rerio (zebrafish)	gspt1	NP_001003992.1
Kluyveromyces lactis	KLLA0D17424g	XP_453831.1
Oryza sativa (rice)	Os04g0270100	NP_001052341.1
	LOC716860	XP_001107851.2
Bos taurus (cattle)	GSPT1	NP_001091540.2
Mus musculus (house mouse)	Gspt1	NP_001123480.1
Rattus norvegicus (Norway rat)	LOC100911685	XP_006245802.1
Eremothecium gossypii	AGOS_AGL145W	NP_986522.2
Danio rerio (zebrafish)	gspt1l	NP_942101.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009310	XP_320105.3
Schizosaccharomyces pombe (fission yeast)	sup35	NP_588225.1
Neurospora crassa	NCU04790	XP_960416.1
Arabidopsis thaliana (thale cress)	AT1G18070	NP_001185026.1
Canis lupus familiaris (dog)	GSPT1	XP_005621589.1
Drosophila melanogaster (fruit fly)	Elf	NP_001260415.1
Magnaporthe oryzae (rice blast fungus)	MGG_00449	XP_003718618.1
Xenopus tropicalis (tropical clawed frog)	gspt1	NP_001015805.2

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.
Figaro S, Scrima N, Buckingham RH, Heurgu?-Hamard V
FEBS letters582(16)2352-6(2008 Jul)

The effect of eukaryotic release factor depletion on translation termination in human cell lines.
Janzen DM, Geballe AP
Nucleic acids research32(15)4491-502(2004)

The polypeptide chain-releasing factor GSPT1/eRF3 is proteolytically processed into an IAP-binding protein.
Hegde R, Srinivasula SM, Datta P, Madesh M, Wassell R, Zhang Z, Cheong N, Nejmeh J, Fernandes-Alnemri T, Hoshino S, Alnemri ES
The Journal of biological chemistry278(40)38699-706(2003 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Gspt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gspt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09910	NM_001003978.1 Latest version!	Rattus norvegicus G1 to S phase transition 1 (Gspt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa32708	XM_017596996.1 Latest version!	Rattus norvegicus G1 to S phase transition 1 (Gspt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gspt1 ( NM_001003978.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001003978.1 Rattus norvegicus G1 to S phase transition 1 (Gspt1), mRNA.

Price & Availability↑

CloneID	ORa09910
Clone ID Related Accession (Same CDS sequence)	NM_001003978.1
Accession Version	NM_001003978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079092.1. On Aug 30, 2004 this sequence version replaced XM_220151.2. ##Evidence-Data-START## Transcript exon combination :: BC079092.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATCCCA GCAGCGGAGG TGGTGGCGGA GGCGGCGGCG GCGGCGGCGG GAGCAGCAGC 
AGCAGTAGCG ACTCTGCGCC CGACTGCTGG GACCAGACCG ATATGGAGGC TCCCGGGCCG 
GGGCCTTGCG GCGGTGGCTC CATGGCCGCG GTGGCCGAGG CCCAGCGTGA GAACCTCAGC 
GCGGCTTTCA GCCGGCAACT CAACGTCAAC GCCAAGCCCT TCGTCCCCAA CGTCCACGCC 
GCGGAATTCG TGCCGTCTTT CCTGCGAGGT CCGGCCCAGC CGCCGCTATC CCCGGCTGGC 
GCGGCCGGCG GTGACCACGG AGCGGGCAGC AGCGCGGGAG GCCCTTCGGA ACCTGTGGAG 
TCCTCTCAAG AGCAGTCATT GTGTGAAGGT TCAAATTCTA CTGTTAGCAT GGAACTTTCA 
GAACCTGTTG TAGAAAATGG AGAGACAGAA ATGTCCCCAG AAGAATCATG GGAGCACAAA 
GAAGAAATAA GTGAAGCCGA GCCAGCAGGT GGTGGTTCCT CAGGAGATGG AAGGCCACCA 
GAGGAAAGCA CCCAAGAAAT GATGGAGGAG GAAGAAGAAA TACCAAAACC TAAGTCAGTG 
GTGGCACCAC CAGGCGCTCC TAAGAAAGAG CATGTAAATG TAGTGTTCAT CGGGCATGTG 
GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTCGACAAA 
AGAACACTTG AGAAATATGA ACGAGAAGCT AAAGAAAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACAAA TCAGGAAGAG CGAGACAAGG GTAAAACAGT AGAGGTGGGC 
CGTGCCTATT TTGAAACGGA AAAAAAGCAT TTTACAATCC TAGATGCTCC TGGCCATAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ACTTGGCTGT ACTGGTAATC 
TCAGCCCGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCGATGTTGG CAAAGACAGC AGGTGTCAAA CACTTAATCG TGCTTATTAA TAAGATGGAT 
GATCCAACAG TAAACTGGAG CAATGAGAGA TATGAAGAAT GCAAAGAGAA ATTAATACCA 
TTTTTAAAGA AAGTTGGCTT CAATCCCAAA AAGGACATTC ATTTTATGCC CTGCTCAGGA 
CTGACAGGAG CAAATCTTAA AGAGCAATCA GATTTCTGTC CTTGGTACAT TGGCTTACCA 
TTTATTCCAT ATCTGGATAA TTTGCCAAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TGGATAAGTA CAAGGATATG GGGACTGTGG TCCTGGGAAA GCTGGAATCT 
GGATCCATCT GTAAAGGCCA GCAGCTCGTA ATGATGCCCA ATAAGCACAA CGTGGAGGTC 
CTTGGAATAC TTTCTGATGA TGTAGAGACG GACTCTGTAG CTCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGGATTGA AGAGGAAGAG ATTCTTCCAG GATTCATCCT CTGTGATCTT 
AATAATCTTT GTCATTCTGG ACGCACATTC GATGCCCAGA TAGTGATTAT AGAGCACAAG 
TCCATCATCT GCCCAGGGTA TAATGCGGTG CTGCACATTC ACACCTGTAT CGAGGAAGTC 
GAGATAACGG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACTCGA 
CCCCGTTTTG TAAAGCAAGA TCAAGTATGC ATTGCTCGTT TAAGGACAGC AGGAACCATC 
TGCCTGGAGA CCTTTAAGGA CTTCCCTCAG ATGGGACGTT TTACCTTAAG AGATGAAGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003978.1
CDS	195..2105
Translation	MDPSSGGGGGGGGGGGGSSSSSSDSAPDCWDQTDMEAPGPGPCGGGSMAAVAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAQPPLSPAGAAGGDHGAGSSAGGPSEPVESSQEQSLCEGSNSTVSMELSEPVVENGETEMSPEESWEHKEEISEAEPAGGGSSGDGRPPEESTQEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLIPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDSVAPGENLKIRLKGIEEEEILPGFILCDLNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	NM_001003978.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G1 to S phase transition 1 (Gspt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003978.1

ORF Insert Sequence:

ATGGATCCCA GCAGCGGAGG TGGTGGCGGA GGCGGCGGCG GCGGCGGCGG GAGCAGCAGC 
AGCAGTAGCG ACTCTGCGCC CGACTGCTGG GACCAGACCG ATATGGAGGC TCCCGGGCCG 
GGGCCTTGCG GCGGTGGCTC CATGGCCGCG GTGGCCGAGG CCCAGCGTGA GAACCTCAGC 
GCGGCTTTCA GCCGGCAACT CAACGTCAAC GCCAAGCCCT TCGTCCCCAA CGTCCACGCC 
GCGGAATTCG TGCCGTCTTT CCTGCGAGGT CCGGCCCAGC CGCCGCTATC CCCGGCTGGC 
GCGGCCGGCG GTGACCACGG AGCGGGCAGC AGCGCGGGAG GCCCTTCGGA ACCTGTGGAG 
TCCTCTCAAG AGCAGTCATT GTGTGAAGGT TCAAATTCTA CTGTTAGCAT GGAACTTTCA 
GAACCTGTTG TAGAAAATGG AGAGACAGAA ATGTCCCCAG AAGAATCATG GGAGCACAAA 
GAAGAAATAA GTGAAGCCGA GCCAGCAGGT GGTGGTTCCT CAGGAGATGG AAGGCCACCA 
GAGGAAAGCA CCCAAGAAAT GATGGAGGAG GAAGAAGAAA TACCAAAACC TAAGTCAGTG 
GTGGCACCAC CAGGCGCTCC TAAGAAAGAG CATGTAAATG TAGTGTTCAT CGGGCATGTG 
GATGCTGGCA AGTCAACCAT TGGAGGACAA ATAATGTATT TGACTGGAAT GGTCGACAAA 
AGAACACTTG AGAAATATGA ACGAGAAGCT AAAGAAAAAA ACAGAGAAAC TTGGTACTTG 
TCTTGGGCCT TAGACACAAA TCAGGAAGAG CGAGACAAGG GTAAAACAGT AGAGGTGGGC 
CGTGCCTATT TTGAAACGGA AAAAAAGCAT TTTACAATCC TAGATGCTCC TGGCCATAAG 
AGTTTTGTCC CAAATATGAT TGGTGGTGCC TCTCAAGCTG ACTTGGCTGT ACTGGTAATC 
TCAGCCCGGA AAGGAGAGTT TGAAACTGGA TTTGAAAAAG GAGGACAGAC AAGAGAACAT 
GCGATGTTGG CAAAGACAGC AGGTGTCAAA CACTTAATCG TGCTTATTAA TAAGATGGAT 
GATCCAACAG TAAACTGGAG CAATGAGAGA TATGAAGAAT GCAAAGAGAA ATTAATACCA 
TTTTTAAAGA AAGTTGGCTT CAATCCCAAA AAGGACATTC ATTTTATGCC CTGCTCAGGA 
CTGACAGGAG CAAATCTTAA AGAGCAATCA GATTTCTGTC CTTGGTACAT TGGCTTACCA 
TTTATTCCAT ATCTGGATAA TTTGCCAAAC TTCAATAGAT CAGTTGATGG ACCAATCAGG 
CTGCCAATTG TGGATAAGTA CAAGGATATG GGGACTGTGG TCCTGGGAAA GCTGGAATCT 
GGATCCATCT GTAAAGGCCA GCAGCTCGTA ATGATGCCCA ATAAGCACAA CGTGGAGGTC 
CTTGGAATAC TTTCTGATGA TGTAGAGACG GACTCTGTAG CTCCAGGTGA AAACCTCAAA 
ATCAGACTGA AAGGGATTGA AGAGGAAGAG ATTCTTCCAG GATTCATCCT CTGTGATCTT 
AATAATCTTT GTCATTCTGG ACGCACATTC GATGCCCAGA TAGTGATTAT AGAGCACAAG 
TCCATCATCT GCCCAGGGTA TAATGCGGTG CTGCACATTC ACACCTGTAT CGAGGAAGTC 
GAGATAACGG CCTTAATCTG CTTGGTAGAC AAAAAATCAG GAGAAAAAAG TAAGACTCGA 
CCCCGTTTTG TAAAGCAAGA TCAAGTATGC ATTGCTCGTT TAAGGACAGC AGGAACCATC 
TGCCTGGAGA CCTTTAAGGA CTTCCCTCAG ATGGGACGTT TTACCTTAAG AGATGAAGGT 
AAGACCATTG CAATTGGAAA AGTTCTGAAA CTGGTTCCAG AGAAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gspt1 ( XM_017596996.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452485.1 Rattus norvegicus G1 to S phase transition 1 (Gspt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32708
Clone ID Related Accession (Same CDS sequence)	XM_017596996.1
Accession Version	XM_017596996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	eukaryotic peptide chain release factor GTP-binding subunit ERF3A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCCA GCAGCGGAGG TGGTGGCGGA GGCGGCGGCG GCGGCGGCGG GAGCAGCAGC 
AGCAGTAGCG ACTCTGCGCC CGACTGCTGG GACCAGACCG ATATGGAGGC TCCCGGGCCG 
GGGCCTTGCG GCGGTGGCTC CATGGCCGCG GTGGCCGAGG CCCAGCGTGA GAACCTCAGC 
GCGGCTTTCA GCCGGCAACT CAACGTCAAC GCCAAGCCCT TCGTCCCCAA CGTCCACGCC 
GCGGAATTCG TGCCGTCTTT CCTGCGAGGT CCGGCCCAGC CGCCGCTATC CCCGGCTGGC 
GCGGCCGGCG GTGACCACGG AGCGGGCAGC AGCGCGGGAG GCCCTTCGGA ACCTGTGGAG 
TCCTCTCAAG AGCAGTCATT GTGTGAAGGT TCAAATTCTA CTGTTAGCAT GGAACTTTCA 
GAACCTGTTG AAAATGGAGA GACAGAAATG TCCCCAGAAG AATCATGGGA GCACAAAGAA 
GAAATAAGTG AAGCCGAGCC AGCAGGTGGT GGTTCCTCAG GAGATGGAAG GCCACCAGAG 
GAAAGCACCC AAGAAATGAT GGAGGAGGAA GAAGAAATAC CAAAACCTAA GTCAGTGGTG 
GCACCACCAG GCGCTCCTAA GAAAGAGCAT GTAAATGTAG TGTTCATCGG GCATGTGGAT 
GCTGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT CGACAAAAGA 
ACACTTGAGA AATATGAACG AGAAGCTAAA GAAAAAAACA GAGAAACTTG GTACTTGTCT 
TGGGCCTTAG ACACAAATCA GGAAGAGCGA GACAAGGGTA AAACAGTAGA GGTGGGCCGT 
GCCTATTTTG AAACGGAAAA AAAGCATTTT ACAATCCTAG ATGCTCCTGG CCATAAGAGT 
TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGACT TGGCTGTACT GGTAATCTCA 
GCCCGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG AGAACATGCG 
ATGTTGGCAA AGACAGCAGG TGTCAAACAC TTAATCGTGC TTATTAATAA GATGGATGAT 
CCAACAGTAA ACTGGAGCAA TGAGAGATAT GAAGAATGCA AAGAGAAATT AATACCATTT 
TTAAAGAAAG TTGGCTTCAA TCCCAAAAAG GACATTCATT TTATGCCCTG CTCAGGACTG 
ACAGGAGCAA ATCTTAAAGA GCAATCAGAT TTCTGTCCTT GGTACATTGG CTTACCATTT 
ATTCCATATC TGGATAATTT GCCAAACTTC AATAGATCAG TTGATGGACC AATCAGGCTG 
CCAATTGTGG ATAAGTACAA GGATATGGGG ACTGTGGTCC TGGGAAAGCT GGAATCTGGA 
TCCATCTGTA AAGGCCAGCA GCTCGTAATG ATGCCCAATA AGCACAACGT GGAGGTCCTT 
GGAATACTTT CTGATGATGT AGAGACGGAC TCTGTAGCTC CAGGTGAAAA CCTCAAAATC 
AGACTGAAAG GGATTGAAGA GGAAGAGATT CTTCCAGGAT TCATCCTCTG TGATCTTAAT 
AATCTTTGTC ATTCTGGACG CACATTCGAT GCCCAGATAG TGATTATAGA GCACAAGTCC 
ATCATCTGCC CAGGGTATAA TGCGGTGCTG CACATTCACA CCTGTATCGA GGAAGTCGAG 
ATAACGGCCT TAATCTGCTT GGTAGACAAA AAATCAGGAG AAAAAAGTAA GACTCGACCC 
CGTTTTGTAA AGCAAGATCA AGTATGCATT GCTCGTTTAA GGACAGCAGG AACCATCTGC 
CTGGAGACCT TTAAGGACTT CCCTCAGATG GGACGTTTTA CCTTAAGAGA TGAAGGTAAG 
ACCATTGCAA TTGGAAAAGT TCTGAAACTG GTTCCAGAGA AAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452485.1
CDS	1..1908
Translation	MDPSSGGGGGGGGGGGGSSSSSSDSAPDCWDQTDMEAPGPGPCGGGSMAAVAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAQPPLSPAGAAGGDHGAGSSAGGPSEPVESSQEQSLCEGSNSTVSMELSEPVENGETEMSPEESWEHKEEISEAEPAGGGSSGDGRPPEESTQEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLIPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDSVAPGENLKIRLKGIEEEEILPGFILCDLNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD

Target ORF information:

RefSeq Version	XM_017596996.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G1 to S phase transition 1 (Gspt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596996.1

ORF Insert Sequence:

ATGGATCCCA GCAGCGGAGG TGGTGGCGGA GGCGGCGGCG GCGGCGGCGG GAGCAGCAGC 
AGCAGTAGCG ACTCTGCGCC CGACTGCTGG GACCAGACCG ATATGGAGGC TCCCGGGCCG 
GGGCCTTGCG GCGGTGGCTC CATGGCCGCG GTGGCCGAGG CCCAGCGTGA GAACCTCAGC 
GCGGCTTTCA GCCGGCAACT CAACGTCAAC GCCAAGCCCT TCGTCCCCAA CGTCCACGCC 
GCGGAATTCG TGCCGTCTTT CCTGCGAGGT CCGGCCCAGC CGCCGCTATC CCCGGCTGGC 
GCGGCCGGCG GTGACCACGG AGCGGGCAGC AGCGCGGGAG GCCCTTCGGA ACCTGTGGAG 
TCCTCTCAAG AGCAGTCATT GTGTGAAGGT TCAAATTCTA CTGTTAGCAT GGAACTTTCA 
GAACCTGTTG AAAATGGAGA GACAGAAATG TCCCCAGAAG AATCATGGGA GCACAAAGAA 
GAAATAAGTG AAGCCGAGCC AGCAGGTGGT GGTTCCTCAG GAGATGGAAG GCCACCAGAG 
GAAAGCACCC AAGAAATGAT GGAGGAGGAA GAAGAAATAC CAAAACCTAA GTCAGTGGTG 
GCACCACCAG GCGCTCCTAA GAAAGAGCAT GTAAATGTAG TGTTCATCGG GCATGTGGAT 
GCTGGCAAGT CAACCATTGG AGGACAAATA ATGTATTTGA CTGGAATGGT CGACAAAAGA 
ACACTTGAGA AATATGAACG AGAAGCTAAA GAAAAAAACA GAGAAACTTG GTACTTGTCT 
TGGGCCTTAG ACACAAATCA GGAAGAGCGA GACAAGGGTA AAACAGTAGA GGTGGGCCGT 
GCCTATTTTG AAACGGAAAA AAAGCATTTT ACAATCCTAG ATGCTCCTGG CCATAAGAGT 
TTTGTCCCAA ATATGATTGG TGGTGCCTCT CAAGCTGACT TGGCTGTACT GGTAATCTCA 
GCCCGGAAAG GAGAGTTTGA AACTGGATTT GAAAAAGGAG GACAGACAAG AGAACATGCG 
ATGTTGGCAA AGACAGCAGG TGTCAAACAC TTAATCGTGC TTATTAATAA GATGGATGAT 
CCAACAGTAA ACTGGAGCAA TGAGAGATAT GAAGAATGCA AAGAGAAATT AATACCATTT 
TTAAAGAAAG TTGGCTTCAA TCCCAAAAAG GACATTCATT TTATGCCCTG CTCAGGACTG 
ACAGGAGCAA ATCTTAAAGA GCAATCAGAT TTCTGTCCTT GGTACATTGG CTTACCATTT 
ATTCCATATC TGGATAATTT GCCAAACTTC AATAGATCAG TTGATGGACC AATCAGGCTG 
CCAATTGTGG ATAAGTACAA GGATATGGGG ACTGTGGTCC TGGGAAAGCT GGAATCTGGA 
TCCATCTGTA AAGGCCAGCA GCTCGTAATG ATGCCCAATA AGCACAACGT GGAGGTCCTT 
GGAATACTTT CTGATGATGT AGAGACGGAC TCTGTAGCTC CAGGTGAAAA CCTCAAAATC 
AGACTGAAAG GGATTGAAGA GGAAGAGATT CTTCCAGGAT TCATCCTCTG TGATCTTAAT 
AATCTTTGTC ATTCTGGACG CACATTCGAT GCCCAGATAG TGATTATAGA GCACAAGTCC 
ATCATCTGCC CAGGGTATAA TGCGGTGCTG CACATTCACA CCTGTATCGA GGAAGTCGAG 
ATAACGGCCT TAATCTGCTT GGTAGACAAA AAATCAGGAG AAAAAAGTAA GACTCGACCC 
CGTTTTGTAA AGCAAGATCA AGTATGCATT GCTCGTTTAA GGACAGCAGG AACCATCTGC 
CTGGAGACCT TTAAGGACTT CCCTCAGATG GGACGTTTTA CCTTAAGAGA TGAAGGTAAG 
ACCATTGCAA TTGGAAAAGT TCTGAAACTG GTTCCAGAGA AAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.
FEBS letters582(16)2352-6(2008 Jul)
Figaro S,Scrima N,Buckingham RH,Heurgu?-Hamard V

The effect of eukaryotic release factor depletion on translation termination in human cell lines.
Nucleic acids research32(15)4491-502(2004)
Janzen DM,Geballe AP

The polypeptide chain-releasing factor GSPT1/eRF3 is proteolytically processed into an IAP-binding protein.
The Journal of biological chemistry278(40)38699-706(2003 Oct)
Hegde R,Srinivasula SM,Datta P,Madesh M,Wassell R,Zhang Z,Cheong N,Nejmeh J,Fernandes-Alnemri T,Hoshino S,Alnemri ES

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Gspt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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