Grm2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grm2
Entrez Gene ID	24415
Full Name	glutamate metabotropic receptor 2
General protein information	Preferred Names glutamate metabotropic receptor 2 Names metabotropic glutamate receptor 2 glutamate receptor, metabotropic 2 mGluR2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	mediates synaptic transmission; may be involved in brain microvascular function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001105711.1	NP_001099181.1	metabotropic glutamate receptor 2 precursor
XM_017595456.1	XP_017450945.1	metabotropic glutamate receptor 2 isoform X1
XM_017595457.1	XP_017450946.1	metabotropic glutamate receptor 2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GRM2	XP_541867.2
Xenopus tropicalis (tropical clawed frog)	grm2	XP_004914127.1
Rattus norvegicus (Norway rat)	Grm2	NP_001099181.1
Mus musculus (house mouse)	Grm2	NP_001153825.1
Gallus gallus (chicken)	GRM2	XP_004944551.1
Homo sapiens (human)	GRM2	NP_000830.2
	GRM2	XP_001095565.1
Bos taurus (cattle)	GRM2	NP_001192795.1
Danio rerio (zebrafish)	grm2a	XP_002662553.3
Danio rerio (zebrafish)	grm7	XP_005172969.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress.
Nasca C, Bigio B, Zelli D, de Angelis P, Lau T, Okamoto M, Soya H, Ni J, Brichta L, Greengard P, Neve RL, Lee FS, McEwen BS
Neuron96(2)402-413.e5(2017 Oct)

Allosteric control of an asymmetric transduction in a G protein-coupled receptor heterodimer.
Liu J, Zhang Z, Moreno-Delgado D, Dalton JA, Rovira X, Trapero A, Goudet C, Llebaria A, Giraldo J, Yuan Q, Rondard P, Huang S, Liu J, Pin JP
eLife6(2017 Aug)

Genetic deletion of mGlu2 metabotropic glutamate receptors improves the short-term outcome of cerebral transient focal ischemia.
Mastroiacovo F, Moyanova S, Cannella M, Gaglione A, Verhaeghe R, Bozza G, Madonna M, Motolese M, Traficante A, Riozzi B, Bruno V, Battaglia G, Lodge D, Nicoletti F
Molecular brain10(1)39(2017 08)

Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1 alpha, mGluR2 and mGluR5, relative to neurotransmitter release sites.
Luj?n R, Roberts JD, Shigemoto R, Ohishi H, Somogyi P
Journal of chemical neuroanatomy13(4)219-41(1997 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Grm2 mutant animals display characteristics of altered emotionality, impulsivity and risk-related behaviors and increased voluntary alcohol intake.
Title: Prevalence and influence of cys407* Grm2 mutation in Hannover-derived Wistar rats: mGlu2 receptor loss links to alcohol intake, risk taking and emotional behaviour.

Findings suggest a time dependent evolving pattern of mGluR2/3 density during the pathogenesis of mesial temporal lobe epilepsy and provide insights for utilizing this data for in vivo imaging to predict the specific time points and responsiveness to the therapy targeting mGluR2/3.
Title: Metabotropic glutamate receptor 2/3 density and its relation to the hippocampal neuropathology in a model of temporal lobe epilepsy in rats.

These data reveal histone modification modulates mGluR2/3 expression in the spinal cord to attenuate stressinduced visceral hypersensitivity.
Title: EXPRESS: Histone hyperacetylation modulates spinal type II metabotropic glutamate receptor alleviating stress-induced visceral hypersensitivity in female rats.

This study demonstrated that the Male prenatal chronic mild stress rats expressed significantly higher mGluR2/3 in the prefrontal cortex but lower in female amygalda.
Title: Prenatal chronic mild stress induces depression-like behavior and sex-specific changes in regional glutamate receptor expression patterns in adult rats.

Glutamate stimulates local protein synthesis in the axons of rat cortical neurons by activating AMPA receptors and metabotropic glutamate receptors.
Title: Glutamate Stimulates Local Protein Synthesis in the Axons of Rat Cortical Neurons by Activating ?-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptors and Metabotropic Glutamate Receptors.

The following Grm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13134	NM_001105711.1 Latest version!	Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa13134	XM_017595457.1 Latest version!	Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa13134	XM_017595456.1 Latest version!	Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grm2 ( NM_001105711.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099181.1 Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), mRNA.

Price & Availability↑

CloneID	ORa13134
Clone ID Related Accession (Same CDS sequence)	NM_001105711.1 , XM_017595456.1 , XM_017595457.1
Accession Version	NM_001105711.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	metabotropic glutamate receptor 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473954.2. On or before Oct 3, 2007 this sequence version replaced XM_001073809.1, XM_343470.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M92075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN02642596 [ECO:0000348] ##Evidence-Data-END##

ATGGAATCAC TGCTTGGGTT TCTGGCACTG CTGCTGCTGT GGGGTGCCGT GGCCGAGGGC 
CCGGCCAAGA AGGTGCTGAC CCTGGAGGGG GACCTGGTGC TGGGTGGGCT GTTCCCAGTG 
CACCAGAAGG GTGGCCCAGC CGAGGAGTGT GGACCTGTTA ATGAGCACCG AGGCATACAG 
CGCCTAGAGG CTATGCTTTT TGCACTGGAC CGCATCAACC GCGACCCCCA CCTGCTGCCT 
GGTGTGCGCT TGGGTGCGCA CATCCTCGAC AGCTGCTCCA AGGATACACA CGCCCTGGAG 
CAGGCGCTGG ACTTTGTGCG TGCCTCACTC AGTCGTGGCG CTGACGGCTC ACGCCACATC 
TGTCCTGATG GCTCCTATGC CACCCACAGT GATGCTCCTA CAGCTGTCAC CGGTGTCATT 
GGTGGCTCCT ACAGTGATGT CTCCATCCAG GTGGCCAATC TCCTGCGGCT GTTCCAGATC 
CCACAGATCA GCTATGCCTC CACCAGTGCC AAGCTGAGTG ACAAGTCCCG TTACGATTAC 
TTTGCTCGCA CTGTGCCCCC AGACTTCTTC CAAGCCAAGG CCATGGCTGA GATTCTCCGC 
TTTTTCAACT GGACATATGT GTCTACGGTG GCATCTGAGG GTGACTATGG TGAGACAGGC 
ATTGAGGCTT TCGAGCTCGA GGCTCGGGCA CGCAACATCT GCGTGGCCAC TTCTGAGAAG 
GTGGGCCGTG CCATGAGCCG CGCTGCCTTC GAGGGCGTGG TGCGAGCCCT GTTGCAGAAA 
CCCAGTGCCC GTGTGGCTGT GCTCTTCACC CGGTCCGAGG ATGCCCGTGA GCTGCTTGCA 
GCCACCCAGC GCCTCAACGC CAGCTTCACA TGGGTGGCCA GCGACGGCTG GGGGGCCCTG 
GAGAGCGTGG TGGCAGGCAG TGAAAGGGCT GCTGAGGGCG CCATCACCAT TGAACTGGCC 
TCCTACCCCA TCAGTGACTT TGCTTCCTAC TTCCAGAGCT TGGATCCGTG GAACAACAGC 
AGAAACCCTT GGTTCCGTGA GTTCTGGGAG GAGAGGTTCC ATTGCAGCTT CCGGCAGCGA 
GACTGTGCCG CCCACTCTCT GCGGGCCGTG CCCTTTGAAC AGGAGTCAAA GATCATGTTT 
GTGGTTAATG CCGTCTATGC CATGGCCCAC GCTCTACACA ACATGCACCG TGCCCTCTGT 
CCCAACACCA CCCACCTCTG CGATGCTATG AGGCCTGTCA ATGGGCGCCG CCTCTACAAA 
GACTTCGTGC TCAATGTCAA GTTTGACGCC CCCTTTCGCC CAGCAGATAC TGACGATGAG 
GTCCGCTTCG ACCGCTTTGG TGACGGTATT GGCCGCTACA ACATCTTCAC CTATCTGCGG 
GCAGGCAGTG GGCGCTATCG CTACCAGAAG GTAGGCTACT GGGCAGAAGG TCTGACTCTG 
GACACTAGCT TCATTCCATG GGCCTCCCCA TCAGCCGGAC CTCTTCCTGC CTCTCGCTGT 
AGCGAGCCCT GCCTTCAGAA CGAGGTGAAG AGCGTGCAGC CGGGCGAGGT CTGCTGTTGG 
CTCTGCATTC CCTGTCAGCC CTATGAGTAC AGGCTGGATG AGTTCACCTG CGCTGACTGT 
GGCCTGGGCT ACTGGCCTAA TGCCAGTCTG ACTGGCTGCT TTGAGCTGCC CCAGGAGTAC 
ATCCGCTGGG GTGATGCCTG GGCGGTGGGA CCTGTCACCA TCGCCTGCCT GGGTGCCCTG 
GCGACACTCT TTGTGTTGGG TGTCTTTGTG AGGCATAATG CCACACCCGT GGTCAAGGCT 
TCCGGTCGGG AGCTTTGCTA CATTCTGCTG GGCGGTGTCT TCCTTTGCTA TTGTATGACC 
TTCGTCTTCA TTGCTAAGCC TTCCACGGCC GTCTGTACCT TGAGGCGCCT CGGTTTGGGT 
ACCGCCTTCT CTGTCTGCTA CTCAGCCCTC CTCACCAAGA CCAATCGCAT TGCTCGCATA 
TTTGGCGGGG CCCGGGAGGG TGCCCAGCGG CCACGCTTCA TCAGTCCCGC CTCACAGGTG 
GCCATCTGCT TGGCACTTAT CTCGGGCCAG CTGCTCATTG TCGCTGCCTG GCTGGTGGTG 
GAGGCACCTG GCACAGGCAA GGAGACAGCC CCTGAACGGC GGGAAGTGGT GACATTGCGC 
TGTAACCACC GTGACGCGAG CATGCTCGGC TCACTGGCCT ACAATGTGCT CCTCATCGCG 
CTCTGCACGC TCTATGCCTT CAAGACCCGC AAGTGCCCGG AGAACTTCAA CGAAGCCAAG 
TTCATCGGCT TCACCATGTA CACCACCTGC ATCATCTGGC TGGCTTTCCT TCCTATCTTC 
TATGTCACCT CCAGTGATTA TCGGGTGCAG ACCACCACGA TGTGCGTGTC CGTCAGCCTC 
AGTGGCTCTG TGGTGCTTGG CTGCCTCTTT GCACCCAAGT TGCACATCAT CCTTTTCCAG 
CCACAGAAGA ATGTGGTGAG CCACCGGGCA CCTACCAGCC GCTTTGGCAG CGCTGCCCCC 
AGGGCCAGCG CCAACCTTGG TCAAGGGTCT GGATCCCAGT TTGTTCCCAC TGTTTGCAAC 
GGCCGTGAGG TGGTGGACTC AACAACGTCG TCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099181.1
CDS	199..2817
Translation	MESLLGFLALLLLWGAVAEGPAKKVLTLEGDLVLGGLFPVHQKGGPAEECGPVNEHRGIQRLEAMLFALDRINRDPHLLPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHSDAPTAVTGVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALLQKPSARVAVLFTRSEDARELLAATQRLNASFTWVASDGWGALESVVAGSERAAEGAITIELASYPISDFASYFQSLDPWNNSRNPWFREFWEERFHCSFRQRDCAAHSLRAVPFEQESKIMFVVNAVYAMAHALHNMHRALCPNTTHLCDAMRPVNGRRLYKDFVLNVKFDAPFRPADTDDEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSFIPWASPSAGPLPASRCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELPQEYIRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYCMTFVFIAKPSTAVCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPASQVAICLALISGQLLIVAAWLVVEAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLLIALCTLYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCVSVSLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAPRASANLGQGSGSQFVPTVCNGREVVDSTTSSL

Target ORF information:

RefSeq Version	NM_001105711.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001105711.1

ORF Insert Sequence:

ATGGAATCAC TGCTTGGGTT TCTGGCACTG CTGCTGCTGT GGGGTGCCGT GGCCGAGGGC 
CCGGCCAAGA AGGTGCTGAC CCTGGAGGGG GACCTGGTGC TGGGTGGGCT GTTCCCAGTG 
CACCAGAAGG GTGGCCCAGC CGAGGAGTGT GGACCTGTTA ATGAGCACCG AGGCATACAG 
CGCCTAGAGG CTATGCTTTT TGCACTGGAC CGCATCAACC GCGACCCCCA CCTGCTGCCT 
GGTGTGCGCT TGGGTGCGCA CATCCTCGAC AGCTGCTCCA AGGATACACA CGCCCTGGAG 
CAGGCGCTGG ACTTTGTGCG TGCCTCACTC AGTCGTGGCG CTGACGGCTC ACGCCACATC 
TGTCCTGATG GCTCCTATGC CACCCACAGT GATGCTCCTA CAGCTGTCAC CGGTGTCATT 
GGTGGCTCCT ACAGTGATGT CTCCATCCAG GTGGCCAATC TCCTGCGGCT GTTCCAGATC 
CCACAGATCA GCTATGCCTC CACCAGTGCC AAGCTGAGTG ACAAGTCCCG TTACGATTAC 
TTTGCTCGCA CTGTGCCCCC AGACTTCTTC CAAGCCAAGG CCATGGCTGA GATTCTCCGC 
TTTTTCAACT GGACATATGT GTCTACGGTG GCATCTGAGG GTGACTATGG TGAGACAGGC 
ATTGAGGCTT TCGAGCTCGA GGCTCGGGCA CGCAACATCT GCGTGGCCAC TTCTGAGAAG 
GTGGGCCGTG CCATGAGCCG CGCTGCCTTC GAGGGCGTGG TGCGAGCCCT GTTGCAGAAA 
CCCAGTGCCC GTGTGGCTGT GCTCTTCACC CGGTCCGAGG ATGCCCGTGA GCTGCTTGCA 
GCCACCCAGC GCCTCAACGC CAGCTTCACA TGGGTGGCCA GCGACGGCTG GGGGGCCCTG 
GAGAGCGTGG TGGCAGGCAG TGAAAGGGCT GCTGAGGGCG CCATCACCAT TGAACTGGCC 
TCCTACCCCA TCAGTGACTT TGCTTCCTAC TTCCAGAGCT TGGATCCGTG GAACAACAGC 
AGAAACCCTT GGTTCCGTGA GTTCTGGGAG GAGAGGTTCC ATTGCAGCTT CCGGCAGCGA 
GACTGTGCCG CCCACTCTCT GCGGGCCGTG CCCTTTGAAC AGGAGTCAAA GATCATGTTT 
GTGGTTAATG CCGTCTATGC CATGGCCCAC GCTCTACACA ACATGCACCG TGCCCTCTGT 
CCCAACACCA CCCACCTCTG CGATGCTATG AGGCCTGTCA ATGGGCGCCG CCTCTACAAA 
GACTTCGTGC TCAATGTCAA GTTTGACGCC CCCTTTCGCC CAGCAGATAC TGACGATGAG 
GTCCGCTTCG ACCGCTTTGG TGACGGTATT GGCCGCTACA ACATCTTCAC CTATCTGCGG 
GCAGGCAGTG GGCGCTATCG CTACCAGAAG GTAGGCTACT GGGCAGAAGG TCTGACTCTG 
GACACTAGCT TCATTCCATG GGCCTCCCCA TCAGCCGGAC CTCTTCCTGC CTCTCGCTGT 
AGCGAGCCCT GCCTTCAGAA CGAGGTGAAG AGCGTGCAGC CGGGCGAGGT CTGCTGTTGG 
CTCTGCATTC CCTGTCAGCC CTATGAGTAC AGGCTGGATG AGTTCACCTG CGCTGACTGT 
GGCCTGGGCT ACTGGCCTAA TGCCAGTCTG ACTGGCTGCT TTGAGCTGCC CCAGGAGTAC 
ATCCGCTGGG GTGATGCCTG GGCGGTGGGA CCTGTCACCA TCGCCTGCCT GGGTGCCCTG 
GCGACACTCT TTGTGTTGGG TGTCTTTGTG AGGCATAATG CCACACCCGT GGTCAAGGCT 
TCCGGTCGGG AGCTTTGCTA CATTCTGCTG GGCGGTGTCT TCCTTTGCTA TTGTATGACC 
TTCGTCTTCA TTGCTAAGCC TTCCACGGCC GTCTGTACCT TGAGGCGCCT CGGTTTGGGT 
ACCGCCTTCT CTGTCTGCTA CTCAGCCCTC CTCACCAAGA CCAATCGCAT TGCTCGCATA 
TTTGGCGGGG CCCGGGAGGG TGCCCAGCGG CCACGCTTCA TCAGTCCCGC CTCACAGGTG 
GCCATCTGCT TGGCACTTAT CTCGGGCCAG CTGCTCATTG TCGCTGCCTG GCTGGTGGTG 
GAGGCACCTG GCACAGGCAA GGAGACAGCC CCTGAACGGC GGGAAGTGGT GACATTGCGC 
TGTAACCACC GTGACGCGAG CATGCTCGGC TCACTGGCCT ACAATGTGCT CCTCATCGCG 
CTCTGCACGC TCTATGCCTT CAAGACCCGC AAGTGCCCGG AGAACTTCAA CGAAGCCAAG 
TTCATCGGCT TCACCATGTA CACCACCTGC ATCATCTGGC TGGCTTTCCT TCCTATCTTC 
TATGTCACCT CCAGTGATTA TCGGGTGCAG ACCACCACGA TGTGCGTGTC CGTCAGCCTC 
AGTGGCTCTG TGGTGCTTGG CTGCCTCTTT GCACCCAAGT TGCACATCAT CCTTTTCCAG 
CCACAGAAGA ATGTGGTGAG CCACCGGGCA CCTACCAGCC GCTTTGGCAG CGCTGCCCCC 
AGGGCCAGCG CCAACCTTGG TCAAGGGTCT GGATCCCAGT TTGTTCCCAC TGTTTGCAAC 
GGCCGTGAGG TGGTGGACTC AACAACGTCG TCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grm2 ( XM_017595456.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450945.1 Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13134
Clone ID Related Accession (Same CDS sequence)	NM_001105711.1 , XM_017595456.1 , XM_017595457.1
Accession Version	XM_017595456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	metabotropic glutamate receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAC TGCTTGGGTT TCTGGCACTG CTGCTGCTGT GGGGTGCCGT GGCCGAGGGC 
CCGGCCAAGA AGGTGCTGAC CCTGGAGGGG GACCTGGTGC TGGGTGGGCT GTTCCCAGTG 
CACCAGAAGG GTGGCCCAGC CGAGGAGTGT GGACCTGTTA ATGAGCACCG AGGCATACAG 
CGCCTAGAGG CTATGCTTTT TGCACTGGAC CGCATCAACC GCGACCCCCA CCTGCTGCCT 
GGTGTGCGCT TGGGTGCGCA CATCCTCGAC AGCTGCTCCA AGGATACACA CGCCCTGGAG 
CAGGCGCTGG ACTTTGTGCG TGCCTCACTC AGTCGTGGCG CTGACGGCTC ACGCCACATC 
TGTCCTGATG GCTCCTATGC CACCCACAGT GATGCTCCTA CAGCTGTCAC CGGTGTCATT 
GGTGGCTCCT ACAGTGATGT CTCCATCCAG GTGGCCAATC TCCTGCGGCT GTTCCAGATC 
CCACAGATCA GCTATGCCTC CACCAGTGCC AAGCTGAGTG ACAAGTCCCG TTACGATTAC 
TTTGCTCGCA CTGTGCCCCC AGACTTCTTC CAAGCCAAGG CCATGGCTGA GATTCTCCGC 
TTTTTCAACT GGACATATGT GTCTACGGTG GCATCTGAGG GTGACTATGG TGAGACAGGC 
ATTGAGGCTT TCGAGCTCGA GGCTCGGGCA CGCAACATCT GCGTGGCCAC TTCTGAGAAG 
GTGGGCCGTG CCATGAGCCG CGCTGCCTTC GAGGGCGTGG TGCGAGCCCT GTTGCAGAAA 
CCCAGTGCCC GTGTGGCTGT GCTCTTCACC CGGTCCGAGG ATGCCCGTGA GCTGCTTGCA 
GCCACCCAGC GCCTCAACGC CAGCTTCACA TGGGTGGCCA GCGACGGCTG GGGGGCCCTG 
GAGAGCGTGG TGGCAGGCAG TGAAAGGGCT GCTGAGGGCG CCATCACCAT TGAACTGGCC 
TCCTACCCCA TCAGTGACTT TGCTTCCTAC TTCCAGAGCT TGGATCCGTG GAACAACAGC 
AGAAACCCTT GGTTCCGTGA GTTCTGGGAG GAGAGGTTCC ATTGCAGCTT CCGGCAGCGA 
GACTGTGCCG CCCACTCTCT GCGGGCCGTG CCCTTTGAAC AGGAGTCAAA GATCATGTTT 
GTGGTTAATG CCGTCTATGC CATGGCCCAC GCTCTACACA ACATGCACCG TGCCCTCTGT 
CCCAACACCA CCCACCTCTG CGATGCTATG AGGCCTGTCA ATGGGCGCCG CCTCTACAAA 
GACTTCGTGC TCAATGTCAA GTTTGACGCC CCCTTTCGCC CAGCAGATAC TGACGATGAG 
GTCCGCTTCG ACCGCTTTGG TGACGGTATT GGCCGCTACA ACATCTTCAC CTATCTGCGG 
GCAGGCAGTG GGCGCTATCG CTACCAGAAG GTAGGCTACT GGGCAGAAGG TCTGACTCTG 
GACACTAGCT TCATTCCATG GGCCTCCCCA TCAGCCGGAC CTCTTCCTGC CTCTCGCTGT 
AGCGAGCCCT GCCTTCAGAA CGAGGTGAAG AGCGTGCAGC CGGGCGAGGT CTGCTGTTGG 
CTCTGCATTC CCTGTCAGCC CTATGAGTAC AGGCTGGATG AGTTCACCTG CGCTGACTGT 
GGCCTGGGCT ACTGGCCTAA TGCCAGTCTG ACTGGCTGCT TTGAGCTGCC CCAGGAGTAC 
ATCCGCTGGG GTGATGCCTG GGCGGTGGGA CCTGTCACCA TCGCCTGCCT GGGTGCCCTG 
GCGACACTCT TTGTGTTGGG TGTCTTTGTG AGGCATAATG CCACACCCGT GGTCAAGGCT 
TCCGGTCGGG AGCTTTGCTA CATTCTGCTG GGCGGTGTCT TCCTTTGCTA TTGTATGACC 
TTCGTCTTCA TTGCTAAGCC TTCCACGGCC GTCTGTACCT TGAGGCGCCT CGGTTTGGGT 
ACCGCCTTCT CTGTCTGCTA CTCAGCCCTC CTCACCAAGA CCAATCGCAT TGCTCGCATA 
TTTGGCGGGG CCCGGGAGGG TGCCCAGCGG CCACGCTTCA TCAGTCCCGC CTCACAGGTG 
GCCATCTGCT TGGCACTTAT CTCGGGCCAG CTGCTCATTG TCGCTGCCTG GCTGGTGGTG 
GAGGCACCTG GCACAGGCAA GGAGACAGCC CCTGAACGGC GGGAAGTGGT GACATTGCGC 
TGTAACCACC GTGACGCGAG CATGCTCGGC TCACTGGCCT ACAATGTGCT CCTCATCGCG 
CTCTGCACGC TCTATGCCTT CAAGACCCGC AAGTGCCCGG AGAACTTCAA CGAAGCCAAG 
TTCATCGGCT TCACCATGTA CACCACCTGC ATCATCTGGC TGGCTTTCCT TCCTATCTTC 
TATGTCACCT CCAGTGATTA TCGGGTGCAG ACCACCACGA TGTGCGTGTC CGTCAGCCTC 
AGTGGCTCTG TGGTGCTTGG CTGCCTCTTT GCACCCAAGT TGCACATCAT CCTTTTCCAG 
CCACAGAAGA ATGTGGTGAG CCACCGGGCA CCTACCAGCC GCTTTGGCAG CGCTGCCCCC 
AGGGCCAGCG CCAACCTTGG TCAAGGGTCT GGATCCCAGT TTGTTCCCAC TGTTTGCAAC 
GGCCGTGAGG TGGTGGACTC AACAACGTCG TCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450945.1
CDS	135..2753
Translation	MESLLGFLALLLLWGAVAEGPAKKVLTLEGDLVLGGLFPVHQKGGPAEECGPVNEHRGIQRLEAMLFALDRINRDPHLLPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHSDAPTAVTGVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALLQKPSARVAVLFTRSEDARELLAATQRLNASFTWVASDGWGALESVVAGSERAAEGAITIELASYPISDFASYFQSLDPWNNSRNPWFREFWEERFHCSFRQRDCAAHSLRAVPFEQESKIMFVVNAVYAMAHALHNMHRALCPNTTHLCDAMRPVNGRRLYKDFVLNVKFDAPFRPADTDDEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSFIPWASPSAGPLPASRCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELPQEYIRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYCMTFVFIAKPSTAVCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPASQVAICLALISGQLLIVAAWLVVEAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLLIALCTLYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCVSVSLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAPRASANLGQGSGSQFVPTVCNGREVVDSTTSSL

Target ORF information:

RefSeq Version	XM_017595456.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595456.1

ORF Insert Sequence:

ATGGAATCAC TGCTTGGGTT TCTGGCACTG CTGCTGCTGT GGGGTGCCGT GGCCGAGGGC 
CCGGCCAAGA AGGTGCTGAC CCTGGAGGGG GACCTGGTGC TGGGTGGGCT GTTCCCAGTG 
CACCAGAAGG GTGGCCCAGC CGAGGAGTGT GGACCTGTTA ATGAGCACCG AGGCATACAG 
CGCCTAGAGG CTATGCTTTT TGCACTGGAC CGCATCAACC GCGACCCCCA CCTGCTGCCT 
GGTGTGCGCT TGGGTGCGCA CATCCTCGAC AGCTGCTCCA AGGATACACA CGCCCTGGAG 
CAGGCGCTGG ACTTTGTGCG TGCCTCACTC AGTCGTGGCG CTGACGGCTC ACGCCACATC 
TGTCCTGATG GCTCCTATGC CACCCACAGT GATGCTCCTA CAGCTGTCAC CGGTGTCATT 
GGTGGCTCCT ACAGTGATGT CTCCATCCAG GTGGCCAATC TCCTGCGGCT GTTCCAGATC 
CCACAGATCA GCTATGCCTC CACCAGTGCC AAGCTGAGTG ACAAGTCCCG TTACGATTAC 
TTTGCTCGCA CTGTGCCCCC AGACTTCTTC CAAGCCAAGG CCATGGCTGA GATTCTCCGC 
TTTTTCAACT GGACATATGT GTCTACGGTG GCATCTGAGG GTGACTATGG TGAGACAGGC 
ATTGAGGCTT TCGAGCTCGA GGCTCGGGCA CGCAACATCT GCGTGGCCAC TTCTGAGAAG 
GTGGGCCGTG CCATGAGCCG CGCTGCCTTC GAGGGCGTGG TGCGAGCCCT GTTGCAGAAA 
CCCAGTGCCC GTGTGGCTGT GCTCTTCACC CGGTCCGAGG ATGCCCGTGA GCTGCTTGCA 
GCCACCCAGC GCCTCAACGC CAGCTTCACA TGGGTGGCCA GCGACGGCTG GGGGGCCCTG 
GAGAGCGTGG TGGCAGGCAG TGAAAGGGCT GCTGAGGGCG CCATCACCAT TGAACTGGCC 
TCCTACCCCA TCAGTGACTT TGCTTCCTAC TTCCAGAGCT TGGATCCGTG GAACAACAGC 
AGAAACCCTT GGTTCCGTGA GTTCTGGGAG GAGAGGTTCC ATTGCAGCTT CCGGCAGCGA 
GACTGTGCCG CCCACTCTCT GCGGGCCGTG CCCTTTGAAC AGGAGTCAAA GATCATGTTT 
GTGGTTAATG CCGTCTATGC CATGGCCCAC GCTCTACACA ACATGCACCG TGCCCTCTGT 
CCCAACACCA CCCACCTCTG CGATGCTATG AGGCCTGTCA ATGGGCGCCG CCTCTACAAA 
GACTTCGTGC TCAATGTCAA GTTTGACGCC CCCTTTCGCC CAGCAGATAC TGACGATGAG 
GTCCGCTTCG ACCGCTTTGG TGACGGTATT GGCCGCTACA ACATCTTCAC CTATCTGCGG 
GCAGGCAGTG GGCGCTATCG CTACCAGAAG GTAGGCTACT GGGCAGAAGG TCTGACTCTG 
GACACTAGCT TCATTCCATG GGCCTCCCCA TCAGCCGGAC CTCTTCCTGC CTCTCGCTGT 
AGCGAGCCCT GCCTTCAGAA CGAGGTGAAG AGCGTGCAGC CGGGCGAGGT CTGCTGTTGG 
CTCTGCATTC CCTGTCAGCC CTATGAGTAC AGGCTGGATG AGTTCACCTG CGCTGACTGT 
GGCCTGGGCT ACTGGCCTAA TGCCAGTCTG ACTGGCTGCT TTGAGCTGCC CCAGGAGTAC 
ATCCGCTGGG GTGATGCCTG GGCGGTGGGA CCTGTCACCA TCGCCTGCCT GGGTGCCCTG 
GCGACACTCT TTGTGTTGGG TGTCTTTGTG AGGCATAATG CCACACCCGT GGTCAAGGCT 
TCCGGTCGGG AGCTTTGCTA CATTCTGCTG GGCGGTGTCT TCCTTTGCTA TTGTATGACC 
TTCGTCTTCA TTGCTAAGCC TTCCACGGCC GTCTGTACCT TGAGGCGCCT CGGTTTGGGT 
ACCGCCTTCT CTGTCTGCTA CTCAGCCCTC CTCACCAAGA CCAATCGCAT TGCTCGCATA 
TTTGGCGGGG CCCGGGAGGG TGCCCAGCGG CCACGCTTCA TCAGTCCCGC CTCACAGGTG 
GCCATCTGCT TGGCACTTAT CTCGGGCCAG CTGCTCATTG TCGCTGCCTG GCTGGTGGTG 
GAGGCACCTG GCACAGGCAA GGAGACAGCC CCTGAACGGC GGGAAGTGGT GACATTGCGC 
TGTAACCACC GTGACGCGAG CATGCTCGGC TCACTGGCCT ACAATGTGCT CCTCATCGCG 
CTCTGCACGC TCTATGCCTT CAAGACCCGC AAGTGCCCGG AGAACTTCAA CGAAGCCAAG 
TTCATCGGCT TCACCATGTA CACCACCTGC ATCATCTGGC TGGCTTTCCT TCCTATCTTC 
TATGTCACCT CCAGTGATTA TCGGGTGCAG ACCACCACGA TGTGCGTGTC CGTCAGCCTC 
AGTGGCTCTG TGGTGCTTGG CTGCCTCTTT GCACCCAAGT TGCACATCAT CCTTTTCCAG 
CCACAGAAGA ATGTGGTGAG CCACCGGGCA CCTACCAGCC GCTTTGGCAG CGCTGCCCCC 
AGGGCCAGCG CCAACCTTGG TCAAGGGTCT GGATCCCAGT TTGTTCCCAC TGTTTGCAAC 
GGCCGTGAGG TGGTGGACTC AACAACGTCG TCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grm2 ( XM_017595457.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450946.1 Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), transcript variant X2, mRNA.

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CloneID	ORa13134
Clone ID Related Accession (Same CDS sequence)	NM_001105711.1 , XM_017595456.1 , XM_017595457.1
Accession Version	XM_017595457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	metabotropic glutamate receptor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAC TGCTTGGGTT TCTGGCACTG CTGCTGCTGT GGGGTGCCGT GGCCGAGGGC 
CCGGCCAAGA AGGTGCTGAC CCTGGAGGGG GACCTGGTGC TGGGTGGGCT GTTCCCAGTG 
CACCAGAAGG GTGGCCCAGC CGAGGAGTGT GGACCTGTTA ATGAGCACCG AGGCATACAG 
CGCCTAGAGG CTATGCTTTT TGCACTGGAC CGCATCAACC GCGACCCCCA CCTGCTGCCT 
GGTGTGCGCT TGGGTGCGCA CATCCTCGAC AGCTGCTCCA AGGATACACA CGCCCTGGAG 
CAGGCGCTGG ACTTTGTGCG TGCCTCACTC AGTCGTGGCG CTGACGGCTC ACGCCACATC 
TGTCCTGATG GCTCCTATGC CACCCACAGT GATGCTCCTA CAGCTGTCAC CGGTGTCATT 
GGTGGCTCCT ACAGTGATGT CTCCATCCAG GTGGCCAATC TCCTGCGGCT GTTCCAGATC 
CCACAGATCA GCTATGCCTC CACCAGTGCC AAGCTGAGTG ACAAGTCCCG TTACGATTAC 
TTTGCTCGCA CTGTGCCCCC AGACTTCTTC CAAGCCAAGG CCATGGCTGA GATTCTCCGC 
TTTTTCAACT GGACATATGT GTCTACGGTG GCATCTGAGG GTGACTATGG TGAGACAGGC 
ATTGAGGCTT TCGAGCTCGA GGCTCGGGCA CGCAACATCT GCGTGGCCAC TTCTGAGAAG 
GTGGGCCGTG CCATGAGCCG CGCTGCCTTC GAGGGCGTGG TGCGAGCCCT GTTGCAGAAA 
CCCAGTGCCC GTGTGGCTGT GCTCTTCACC CGGTCCGAGG ATGCCCGTGA GCTGCTTGCA 
GCCACCCAGC GCCTCAACGC CAGCTTCACA TGGGTGGCCA GCGACGGCTG GGGGGCCCTG 
GAGAGCGTGG TGGCAGGCAG TGAAAGGGCT GCTGAGGGCG CCATCACCAT TGAACTGGCC 
TCCTACCCCA TCAGTGACTT TGCTTCCTAC TTCCAGAGCT TGGATCCGTG GAACAACAGC 
AGAAACCCTT GGTTCCGTGA GTTCTGGGAG GAGAGGTTCC ATTGCAGCTT CCGGCAGCGA 
GACTGTGCCG CCCACTCTCT GCGGGCCGTG CCCTTTGAAC AGGAGTCAAA GATCATGTTT 
GTGGTTAATG CCGTCTATGC CATGGCCCAC GCTCTACACA ACATGCACCG TGCCCTCTGT 
CCCAACACCA CCCACCTCTG CGATGCTATG AGGCCTGTCA ATGGGCGCCG CCTCTACAAA 
GACTTCGTGC TCAATGTCAA GTTTGACGCC CCCTTTCGCC CAGCAGATAC TGACGATGAG 
GTCCGCTTCG ACCGCTTTGG TGACGGTATT GGCCGCTACA ACATCTTCAC CTATCTGCGG 
GCAGGCAGTG GGCGCTATCG CTACCAGAAG GTAGGCTACT GGGCAGAAGG TCTGACTCTG 
GACACTAGCT TCATTCCATG GGCCTCCCCA TCAGCCGGAC CTCTTCCTGC CTCTCGCTGT 
AGCGAGCCCT GCCTTCAGAA CGAGGTGAAG AGCGTGCAGC CGGGCGAGGT CTGCTGTTGG 
CTCTGCATTC CCTGTCAGCC CTATGAGTAC AGGCTGGATG AGTTCACCTG CGCTGACTGT 
GGCCTGGGCT ACTGGCCTAA TGCCAGTCTG ACTGGCTGCT TTGAGCTGCC CCAGGAGTAC 
ATCCGCTGGG GTGATGCCTG GGCGGTGGGA CCTGTCACCA TCGCCTGCCT GGGTGCCCTG 
GCGACACTCT TTGTGTTGGG TGTCTTTGTG AGGCATAATG CCACACCCGT GGTCAAGGCT 
TCCGGTCGGG AGCTTTGCTA CATTCTGCTG GGCGGTGTCT TCCTTTGCTA TTGTATGACC 
TTCGTCTTCA TTGCTAAGCC TTCCACGGCC GTCTGTACCT TGAGGCGCCT CGGTTTGGGT 
ACCGCCTTCT CTGTCTGCTA CTCAGCCCTC CTCACCAAGA CCAATCGCAT TGCTCGCATA 
TTTGGCGGGG CCCGGGAGGG TGCCCAGCGG CCACGCTTCA TCAGTCCCGC CTCACAGGTG 
GCCATCTGCT TGGCACTTAT CTCGGGCCAG CTGCTCATTG TCGCTGCCTG GCTGGTGGTG 
GAGGCACCTG GCACAGGCAA GGAGACAGCC CCTGAACGGC GGGAAGTGGT GACATTGCGC 
TGTAACCACC GTGACGCGAG CATGCTCGGC TCACTGGCCT ACAATGTGCT CCTCATCGCG 
CTCTGCACGC TCTATGCCTT CAAGACCCGC AAGTGCCCGG AGAACTTCAA CGAAGCCAAG 
TTCATCGGCT TCACCATGTA CACCACCTGC ATCATCTGGC TGGCTTTCCT TCCTATCTTC 
TATGTCACCT CCAGTGATTA TCGGGTGCAG ACCACCACGA TGTGCGTGTC CGTCAGCCTC 
AGTGGCTCTG TGGTGCTTGG CTGCCTCTTT GCACCCAAGT TGCACATCAT CCTTTTCCAG 
CCACAGAAGA ATGTGGTGAG CCACCGGGCA CCTACCAGCC GCTTTGGCAG CGCTGCCCCC 
AGGGCCAGCG CCAACCTTGG TCAAGGGTCT GGATCCCAGT TTGTTCCCAC TGTTTGCAAC 
GGCCGTGAGG TGGTGGACTC AACAACGTCG TCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450946.1
CDS	483..3101
Translation	MESLLGFLALLLLWGAVAEGPAKKVLTLEGDLVLGGLFPVHQKGGPAEECGPVNEHRGIQRLEAMLFALDRINRDPHLLPGVRLGAHILDSCSKDTHALEQALDFVRASLSRGADGSRHICPDGSYATHSDAPTAVTGVIGGSYSDVSIQVANLLRLFQIPQISYASTSAKLSDKSRYDYFARTVPPDFFQAKAMAEILRFFNWTYVSTVASEGDYGETGIEAFELEARARNICVATSEKVGRAMSRAAFEGVVRALLQKPSARVAVLFTRSEDARELLAATQRLNASFTWVASDGWGALESVVAGSERAAEGAITIELASYPISDFASYFQSLDPWNNSRNPWFREFWEERFHCSFRQRDCAAHSLRAVPFEQESKIMFVVNAVYAMAHALHNMHRALCPNTTHLCDAMRPVNGRRLYKDFVLNVKFDAPFRPADTDDEVRFDRFGDGIGRYNIFTYLRAGSGRYRYQKVGYWAEGLTLDTSFIPWASPSAGPLPASRCSEPCLQNEVKSVQPGEVCCWLCIPCQPYEYRLDEFTCADCGLGYWPNASLTGCFELPQEYIRWGDAWAVGPVTIACLGALATLFVLGVFVRHNATPVVKASGRELCYILLGGVFLCYCMTFVFIAKPSTAVCTLRRLGLGTAFSVCYSALLTKTNRIARIFGGAREGAQRPRFISPASQVAICLALISGQLLIVAAWLVVEAPGTGKETAPERREVVTLRCNHRDASMLGSLAYNVLLIALCTLYAFKTRKCPENFNEAKFIGFTMYTTCIIWLAFLPIFYVTSSDYRVQTTTMCVSVSLSGSVVLGCLFAPKLHIILFQPQKNVVSHRAPTSRFGSAAPRASANLGQGSGSQFVPTVCNGREVVDSTTSSL

Target ORF information:

RefSeq Version	XM_017595457.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate metabotropic receptor 2 (Grm2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595457.1

ORF Insert Sequence:

ATGGAATCAC TGCTTGGGTT TCTGGCACTG CTGCTGCTGT GGGGTGCCGT GGCCGAGGGC 
CCGGCCAAGA AGGTGCTGAC CCTGGAGGGG GACCTGGTGC TGGGTGGGCT GTTCCCAGTG 
CACCAGAAGG GTGGCCCAGC CGAGGAGTGT GGACCTGTTA ATGAGCACCG AGGCATACAG 
CGCCTAGAGG CTATGCTTTT TGCACTGGAC CGCATCAACC GCGACCCCCA CCTGCTGCCT 
GGTGTGCGCT TGGGTGCGCA CATCCTCGAC AGCTGCTCCA AGGATACACA CGCCCTGGAG 
CAGGCGCTGG ACTTTGTGCG TGCCTCACTC AGTCGTGGCG CTGACGGCTC ACGCCACATC 
TGTCCTGATG GCTCCTATGC CACCCACAGT GATGCTCCTA CAGCTGTCAC CGGTGTCATT 
GGTGGCTCCT ACAGTGATGT CTCCATCCAG GTGGCCAATC TCCTGCGGCT GTTCCAGATC 
CCACAGATCA GCTATGCCTC CACCAGTGCC AAGCTGAGTG ACAAGTCCCG TTACGATTAC 
TTTGCTCGCA CTGTGCCCCC AGACTTCTTC CAAGCCAAGG CCATGGCTGA GATTCTCCGC 
TTTTTCAACT GGACATATGT GTCTACGGTG GCATCTGAGG GTGACTATGG TGAGACAGGC 
ATTGAGGCTT TCGAGCTCGA GGCTCGGGCA CGCAACATCT GCGTGGCCAC TTCTGAGAAG 
GTGGGCCGTG CCATGAGCCG CGCTGCCTTC GAGGGCGTGG TGCGAGCCCT GTTGCAGAAA 
CCCAGTGCCC GTGTGGCTGT GCTCTTCACC CGGTCCGAGG ATGCCCGTGA GCTGCTTGCA 
GCCACCCAGC GCCTCAACGC CAGCTTCACA TGGGTGGCCA GCGACGGCTG GGGGGCCCTG 
GAGAGCGTGG TGGCAGGCAG TGAAAGGGCT GCTGAGGGCG CCATCACCAT TGAACTGGCC 
TCCTACCCCA TCAGTGACTT TGCTTCCTAC TTCCAGAGCT TGGATCCGTG GAACAACAGC 
AGAAACCCTT GGTTCCGTGA GTTCTGGGAG GAGAGGTTCC ATTGCAGCTT CCGGCAGCGA 
GACTGTGCCG CCCACTCTCT GCGGGCCGTG CCCTTTGAAC AGGAGTCAAA GATCATGTTT 
GTGGTTAATG CCGTCTATGC CATGGCCCAC GCTCTACACA ACATGCACCG TGCCCTCTGT 
CCCAACACCA CCCACCTCTG CGATGCTATG AGGCCTGTCA ATGGGCGCCG CCTCTACAAA 
GACTTCGTGC TCAATGTCAA GTTTGACGCC CCCTTTCGCC CAGCAGATAC TGACGATGAG 
GTCCGCTTCG ACCGCTTTGG TGACGGTATT GGCCGCTACA ACATCTTCAC CTATCTGCGG 
GCAGGCAGTG GGCGCTATCG CTACCAGAAG GTAGGCTACT GGGCAGAAGG TCTGACTCTG 
GACACTAGCT TCATTCCATG GGCCTCCCCA TCAGCCGGAC CTCTTCCTGC CTCTCGCTGT 
AGCGAGCCCT GCCTTCAGAA CGAGGTGAAG AGCGTGCAGC CGGGCGAGGT CTGCTGTTGG 
CTCTGCATTC CCTGTCAGCC CTATGAGTAC AGGCTGGATG AGTTCACCTG CGCTGACTGT 
GGCCTGGGCT ACTGGCCTAA TGCCAGTCTG ACTGGCTGCT TTGAGCTGCC CCAGGAGTAC 
ATCCGCTGGG GTGATGCCTG GGCGGTGGGA CCTGTCACCA TCGCCTGCCT GGGTGCCCTG 
GCGACACTCT TTGTGTTGGG TGTCTTTGTG AGGCATAATG CCACACCCGT GGTCAAGGCT 
TCCGGTCGGG AGCTTTGCTA CATTCTGCTG GGCGGTGTCT TCCTTTGCTA TTGTATGACC 
TTCGTCTTCA TTGCTAAGCC TTCCACGGCC GTCTGTACCT TGAGGCGCCT CGGTTTGGGT 
ACCGCCTTCT CTGTCTGCTA CTCAGCCCTC CTCACCAAGA CCAATCGCAT TGCTCGCATA 
TTTGGCGGGG CCCGGGAGGG TGCCCAGCGG CCACGCTTCA TCAGTCCCGC CTCACAGGTG 
GCCATCTGCT TGGCACTTAT CTCGGGCCAG CTGCTCATTG TCGCTGCCTG GCTGGTGGTG 
GAGGCACCTG GCACAGGCAA GGAGACAGCC CCTGAACGGC GGGAAGTGGT GACATTGCGC 
TGTAACCACC GTGACGCGAG CATGCTCGGC TCACTGGCCT ACAATGTGCT CCTCATCGCG 
CTCTGCACGC TCTATGCCTT CAAGACCCGC AAGTGCCCGG AGAACTTCAA CGAAGCCAAG 
TTCATCGGCT TCACCATGTA CACCACCTGC ATCATCTGGC TGGCTTTCCT TCCTATCTTC 
TATGTCACCT CCAGTGATTA TCGGGTGCAG ACCACCACGA TGTGCGTGTC CGTCAGCCTC 
AGTGGCTCTG TGGTGCTTGG CTGCCTCTTT GCACCCAAGT TGCACATCAT CCTTTTCCAG 
CCACAGAAGA ATGTGGTGAG CCACCGGGCA CCTACCAGCC GCTTTGGCAG CGCTGCCCCC 
AGGGCCAGCG CCAACCTTGG TCAAGGGTCT GGATCCCAGT TTGTTCCCAC TGTTTGCAAC 
GGCCGTGAGG TGGTGGACTC AACAACGTCG TCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress.
Neuron96(2)402-413.e5(2017 Oct)
Nasca C,Bigio B,Zelli D,de Angelis P,Lau T,Okamoto M,Soya H,Ni J,Brichta L,Greengard P,Neve RL,Lee FS,McEwen BS

Allosteric control of an asymmetric transduction in a G protein-coupled receptor heterodimer.
eLife6(2017 Aug)
Liu J,Zhang Z,Moreno-Delgado D,Dalton JA,Rovira X,Trapero A,Goudet C,Llebaria A,Giraldo J,Yuan Q,Rondard P,Huang S,Liu J,Pin JP

Genetic deletion of mGlu2 metabotropic glutamate receptors improves the short-term outcome of cerebral transient focal ischemia.
Molecular brain10(1)39(2017 08)
Mastroiacovo F,Moyanova S,Cannella M,Gaglione A,Verhaeghe R,Bozza G,Madonna M,Motolese M,Traficante A,Riozzi B,Bruno V,Battaglia G,Lodge D,Nicoletti F

Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1 alpha, mGluR2 and mGluR5, relative to neurotransmitter release sites.
Journal of chemical neuroanatomy13(4)219-41(1997 Oct)
Luj?n R,Roberts JD,Shigemoto R,Ohishi H,Somogyi P

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Grm2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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