Grik5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grik5
Entrez Gene ID	24407
Full Name	glutamate ionotropic receptor kainate type subunit 5
Synonyms	GluK5,iGlu5
General protein information	Preferred Names glutamate ionotropic receptor kainate type subunit 5 Names glutamate receptor ionotropic, kainate 5 KA2 glutamate receptor KA-2 glutamate receptor KA2 glutamate receptor, ionotropic, kainate 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	glutamate receptor subunit KA2; associates with subunits of the GluR5-7 family to form heteromeric kainate-preferring glutamate receptpors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006228382.3	XP_006228444.1	glutamate receptor ionotropic, kainate 5 isoform X1
XM_008758889.2	XP_008757111.1	glutamate receptor ionotropic, kainate 5 isoform X2
NM_031508.2	NP_113696.1	glutamate receptor ionotropic, kainate 5 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Grik5	NP_032194.2
Danio rerio (zebrafish)	grik5	NP_001232890.1
Homo sapiens (human)	GRIK5	NP_002079.3
	LOC706629	XP_002808240.1
Xenopus tropicalis (tropical clawed frog)	grik5	XP_002936446.1
Pan troglodytes (chimpanzee)	GRIK5	XP_524469.3
Canis lupus familiaris (dog)	GRIK5	XP_005616574.1
Rattus norvegicus (Norway rat)	Grik5	NP_113696.1

Gene Ontology
Bibliography

Related articles in PubMed

Agonist binding to the GluK5 subunit is sufficient for functional surface expression of heteromeric GluK2/GluK5 kainate receptors.
Fisher JL, Housley PR
Cellular and molecular neurobiology33(8)1099-108(2013 Nov)

Selective block of postsynaptic kainate receptors reveals their function at hippocampal mossy fiber synapses.
Pinheiro PS, Lanore F, Veran J, Artinian J, Blanchet C, Cr?pel V, Perrais D, Mulle C
Cerebral cortex (New York, N.Y. : 1991)23(2)323-31(2013 Feb)

CaMKII-dependent phosphorylation of GluK5 mediates plasticity of kainate receptors.
Carta M, Opazo P, Veran J, Athan? A, Choquet D, Coussen F, Mulle C
The EMBO journal32(4)496-510(2013 Feb)

Assembly stoichiometry of the GluK2/GluK5 kainate receptor complex.
Reiner A, Arant RJ, Isacoff EY
Cell reports1(3)234-40(2012 Mar)

Distinct functional roles of subunits within the heteromeric kainate receptor.
Fisher JL, Mott DD
The Journal of neuroscience : the official journal of the Society for Neuroscience31(47)17113-22(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that ligand binding to only the GluK5 subunit is both necessary and sufficient to allow trafficking of recombinant GluK2/K5 heteromers to the cell membrane, but that occupancy of the GluK2 site alone is not.
Title: Agonist binding to the GluK5 subunit is sufficient for functional surface expression of heteromeric GluK2/GluK5 kainate receptors.

CaMKII-dependent phosphorylation of GluK5 is responsible for synaptic depression by untrapping of KARs from the PSD and increased diffusion away from synaptic sites.
Title: CaMKII-dependent phosphorylation of GluK5 mediates plasticity of kainate receptors.

GluK2 and GluK5 assemble with 2:2 stoichiometry.
Title: Assembly stoichiometry of the GluK2/GluK5 kainate receptor complex.

Heteromeric GluK2/K5 receptors expressed in human HEK-293T cells show markedly higher glutamate sensitivity than GluK2 homomers and do not desensitize at low glutamate concentrations.
Title: Distinct functional roles of subunits within the heteromeric kainate receptor.

kainic acid-preferring glutamate receptors are responsive to changes in plasma glucose concentrations and may participate in the activation of the paraventricular neurones during hypoglycaemic stress
Title: Provocation of kainic acid receptor mRNA changes in the rat paraventricular nucleus by insulin-induced hypoglycaemia.

The following Grik5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grik5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12125	NM_031508.2 Latest version!	Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa22386	XM_006228382.3 Latest version!	Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa42907	XM_008758889.2 Latest version!	Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grik5 ( NM_031508.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113696.1 Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), mRNA.

Price & Availability↑

CloneID	ORa12125
Clone ID Related Accession (Same CDS sequence)	NM_031508.2
Accession Version	NM_031508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, kainate 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC101853.1 and Z11581.1. On or before Mar 28, 2009 this sequence version replaced NM_017262.1, NM_031508.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z11581.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGCTG AGCTGCTGCT GCTGCTGATA GTCGCCTTCG CCAACCCCAG CTGCCAGGTG 
CTGTCATCAC TGCGCATGGC TGCAATCCTG GACGACCAGA CCGTGTGTGG CCGTGGTGAG 
CGTCTGGCCC TGGCCCTGGC CCGAGAGCAG ATCAATGGGA TCATCGAGGT CCCAGCCAAG 
GCCCGAGTGG AAGTAGACAT CTTTGAGCTG CAACGGGACA GCCAGTACGA GACTACGGAC 
ACCATGTGTC AGATCCTGCC CAAGGGGGTT GTGTCTGTCT TGGGACCCTC CTCCAGCCCA 
GCTTCTGCCT CCACCGTGAG CCATATCTGT GGGGAGAAGG AGATTCCCCA CATCAAGGTA 
GGCCCTGAGG AGACACCCCG CCTTCAGTAC CTCCGCTTTG CATCTGTCAG CCTGTATCCC 
AGTAATGAAG ATGTCAGCCT GGCAGTCTCC CGGATCCTCA AGTCCTTTAA CTACCCCTCC 
GCTAGCCTCA TCTGCGCCAA AGCTGAGTGC CTGCTGCGGT TAGAAGAACT GGTGCGAGGC 
TTCCTCATCT CCAAGGAGAC ACTGTCCGTG AGGATGCTTG ATGACAGCCG GGACCCCACG 
CCGCTACTCA AGGAGATCCG AGATGACAAA GTGTCCACCA TCATCATCGA TGCCAACGCG 
TCCATCTCCC ACCTTGTCCT CCGTAAGGCT TCGGAGCTGG GAATGACCTC AGCGTTTTAC 
AAGTACATCC TCACCACCAT GGACTTCCCC ATCCTGCATC TGGACGGTAT CGTAGAGGAC 
TCCTCCAACA TCTTGGGCTT TTCCATGTTC AACACCTCCC ATCCCTTCTA CCCGGAGTTT 
GTGCGCAGCC TCAACATGTC CTGGAGGGAG AACTGCGAAG CCAGCACCTA CCCTGGCCCT 
GCGCTGTCGG CAGCCCTGAT GTTTGATGCC GTGCATGTGG TGGTAAGCGC TGTCCGAGAG 
CTGAACCGGA GCCAGGAGAT TGGTGTCAAG CCACTGGCTT GCACTTCGGC CAACATCTGG 
CCCCATGGGA CCAGCCTCAT GAACTACCTG CGCATGGTAG AGTATGACGG GCTGACTGGG 
CGGGTTGAGT TCAACAGCAA AGGGCAGAGG ACCAACTACA CACTGCGCAT CCTGGAGAAG 
TCCCGCCAGG GCCACCGTGA GATAGGGGTG TGGTACTCTA ACCGGACCCT GGCCATGAAT 
GCTACCACCC TGGACATCAA CCTGTCACAG ACTCTAGCCA ACAAGACTCT GGTGGTCACA 
ACAATCCTGG AGAACCCGTA TGTCATGCGC CGGCCCAACT TCCAGGCCCT GTCAGGGAAC 
GAGCGCTTTG AGGGCTTCTG CGTGGACATG CTTCGGGAGC TGGCCGAGCT GCTGCGCTTC 
CGGTATCGCC TGCGGTTGGT GGAGGACGGA CTGTACGGGG CACCTGAGCC CAATGGTTCC 
TGGACAGGAA TGGTTGGCGA GCTCATCAAC CGGAAGGCAG ACCTGGCTGT GGCAGCCTTC 
ACCATCACCG CGGAGAGAGA GAAGGTCATC GACTTCTCCA AGCCATTCAT GACCCTGGGG 
ATCAGCATCC TCTACAGGGT GCACATGGGC CGCAAGCCTG GCTACTTCTC CTTCCTGGAC 
CCTTTCTCCC CTGCCGTGTG GCTCTTCATG CTTCTTGCCT ACCTGGCTGT CAGCTGTGTC 
TTGTTTCTGG CTGCCAGGCT GAGCCCTTAT GAGTGGTATA ACCCTCACCC GTGTCTTCGG 
GCGCGTCCCC ATATCCTGGA GAACCAGTAC ACGCTGGGTA ACAGCCTTTG GTTCCCTGTG 
GGTGGCTTCA TGCAGCAGGG CTCGGAGATC ATGCCCCGGG CGCTGTCCAC ACGCTGTGTC 
AGCGGAGTCT GGTGGGCCTT CACCTTGATC ATCATCTCCT CCTACACGGC CAACCTGGCT 
GCCTTCCTCA CGGTGCAGCG CATGGAGGTG CCGGTGGAGT CTGCAGACGA CTTGGCGGAT 
CAGACCAACA TTGAGTACGG CACTATCCAC GCTGGCTCCA CCATGACCTT CTTTCAGAAC 
TCACGGTACC AGACGTACCA GCGGATGTGG AATTACATGC AGTCGAAGCA GCCCAGCGTG 
TTCGTCAAGA GCACAGAGGA GGGGATCGCC CGTGTCCTCA ACTCACGTTA CGCCTTCCTG 
CTGGAGTCCA CCATGAACGA GTACCACAGG CGCCTCAATT GCAACCTCAC CCAGATCGGG 
GGCCTCCTCG ACACCAAGGG CTACGGCATC GGCATGCCGC TGGGCTCCCC ATTCCGGGAT 
GAGATCACGC TGGCCATCCT GCAGCTCCAG GAGAACAACA GGCTGGAGAT CCTGAAGCGC 
AAGTGGTGGG AGGGCGGCCG GTGCCCCAAG GAGGAGGACC ACAGGGCCAA AGGTTTGGGC 
ATGGAGAACA TTGGCGGTAT TTTTGTCGTG CTGATCTGTG GCCTCATCAT TGCTGTCTTC 
GTGGCGGTCA TGGAATTCAT CTGGTCCACG CGGAGGTCAG CGGAGTCTGA GGAGGTGTCG 
GTGTGCCAGG AGATGCTGCA GGAGCTGCGC CACGCCGTGT CTTGCCGCAA GACCTCGCGT 
TCCCGCCGGC GCCGGCGCCC TGGCGGCCCG AGCCGGGCCC TGCTGTCGCT GCGCGCGGTC 
CGCGAGATGC GACTCAGCAA CGGCAAGCTC TACTCGGCCG GCGCGGGCGG GGACGCGGGC 
GCGCACGGGG GTCCGCAGCG CCTCCTGGAC GACCCCGGGC CTCCTGGGGG ACCCCGGCCC 
CAGGCTCCCA CGCCCTGCAC GCACGTGCGC GTCTGCCAGG AGTGCAGGCG CATCCAGGCG 
CTGCGAGCTT CAGGGGCCGG GGCGCCCCCG CGTGGCCTGG GCACTCCGGC CGAAGCCACC 
AGCCCGCCTC GGCCGCGGCC TGGACCCACT GGACCCCGCG AGCTGACTGA GCATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113696.1
CDS	305..3244
Translation	MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIRDDKVSTIIIDANASISHLVLRKASELGMTSAFYKYILTTMDFPILHLDGIVEDSSNILGFSMFNTSHPFYPEFVRSLNMSWRENCEASTYPGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYLRMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVTTILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTGMVGELINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSVFVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRKWWEGGRCPKEEDHRAKGLGMENIGGIFVVLICGLIIAVFVAVMEFIWSTRRSAESEEVSVCQEMLQELRHAVSCRKTSRSRRRRRPGGPSRALLSLRAVREMRLSNGKLYSAGAGGDAGAHGGPQRLLDDPGPPGGPRPQAPTPCTHVRVCQECRRIQALRASGAGAPPRGLGTPAEATSPPRPRPGPTGPRELTEHE

Target ORF information:

RefSeq Version	NM_031508.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031508.2

ORF Insert Sequence:

ATGCCGGCTG AGCTGCTGCT GCTGCTGATA GTCGCCTTCG CCAACCCCAG CTGCCAGGTG 
CTGTCATCAC TGCGCATGGC TGCAATCCTG GACGACCAGA CCGTGTGTGG CCGTGGTGAG 
CGTCTGGCCC TGGCCCTGGC CCGAGAGCAG ATCAATGGGA TCATCGAGGT CCCAGCCAAG 
GCCCGAGTGG AAGTAGACAT CTTTGAGCTG CAACGGGACA GCCAGTACGA GACTACGGAC 
ACCATGTGTC AGATCCTGCC CAAGGGGGTT GTGTCTGTCT TGGGACCCTC CTCCAGCCCA 
GCTTCTGCCT CCACCGTGAG CCATATCTGT GGGGAGAAGG AGATTCCCCA CATCAAGGTA 
GGCCCTGAGG AGACACCCCG CCTTCAGTAC CTCCGCTTTG CATCTGTCAG CCTGTATCCC 
AGTAATGAAG ATGTCAGCCT GGCAGTCTCC CGGATCCTCA AGTCCTTTAA CTACCCCTCC 
GCTAGCCTCA TCTGCGCCAA AGCTGAGTGC CTGCTGCGGT TAGAAGAACT GGTGCGAGGC 
TTCCTCATCT CCAAGGAGAC ACTGTCCGTG AGGATGCTTG ATGACAGCCG GGACCCCACG 
CCGCTACTCA AGGAGATCCG AGATGACAAA GTGTCCACCA TCATCATCGA TGCCAACGCG 
TCCATCTCCC ACCTTGTCCT CCGTAAGGCT TCGGAGCTGG GAATGACCTC AGCGTTTTAC 
AAGTACATCC TCACCACCAT GGACTTCCCC ATCCTGCATC TGGACGGTAT CGTAGAGGAC 
TCCTCCAACA TCTTGGGCTT TTCCATGTTC AACACCTCCC ATCCCTTCTA CCCGGAGTTT 
GTGCGCAGCC TCAACATGTC CTGGAGGGAG AACTGCGAAG CCAGCACCTA CCCTGGCCCT 
GCGCTGTCGG CAGCCCTGAT GTTTGATGCC GTGCATGTGG TGGTAAGCGC TGTCCGAGAG 
CTGAACCGGA GCCAGGAGAT TGGTGTCAAG CCACTGGCTT GCACTTCGGC CAACATCTGG 
CCCCATGGGA CCAGCCTCAT GAACTACCTG CGCATGGTAG AGTATGACGG GCTGACTGGG 
CGGGTTGAGT TCAACAGCAA AGGGCAGAGG ACCAACTACA CACTGCGCAT CCTGGAGAAG 
TCCCGCCAGG GCCACCGTGA GATAGGGGTG TGGTACTCTA ACCGGACCCT GGCCATGAAT 
GCTACCACCC TGGACATCAA CCTGTCACAG ACTCTAGCCA ACAAGACTCT GGTGGTCACA 
ACAATCCTGG AGAACCCGTA TGTCATGCGC CGGCCCAACT TCCAGGCCCT GTCAGGGAAC 
GAGCGCTTTG AGGGCTTCTG CGTGGACATG CTTCGGGAGC TGGCCGAGCT GCTGCGCTTC 
CGGTATCGCC TGCGGTTGGT GGAGGACGGA CTGTACGGGG CACCTGAGCC CAATGGTTCC 
TGGACAGGAA TGGTTGGCGA GCTCATCAAC CGGAAGGCAG ACCTGGCTGT GGCAGCCTTC 
ACCATCACCG CGGAGAGAGA GAAGGTCATC GACTTCTCCA AGCCATTCAT GACCCTGGGG 
ATCAGCATCC TCTACAGGGT GCACATGGGC CGCAAGCCTG GCTACTTCTC CTTCCTGGAC 
CCTTTCTCCC CTGCCGTGTG GCTCTTCATG CTTCTTGCCT ACCTGGCTGT CAGCTGTGTC 
TTGTTTCTGG CTGCCAGGCT GAGCCCTTAT GAGTGGTATA ACCCTCACCC GTGTCTTCGG 
GCGCGTCCCC ATATCCTGGA GAACCAGTAC ACGCTGGGTA ACAGCCTTTG GTTCCCTGTG 
GGTGGCTTCA TGCAGCAGGG CTCGGAGATC ATGCCCCGGG CGCTGTCCAC ACGCTGTGTC 
AGCGGAGTCT GGTGGGCCTT CACCTTGATC ATCATCTCCT CCTACACGGC CAACCTGGCT 
GCCTTCCTCA CGGTGCAGCG CATGGAGGTG CCGGTGGAGT CTGCAGACGA CTTGGCGGAT 
CAGACCAACA TTGAGTACGG CACTATCCAC GCTGGCTCCA CCATGACCTT CTTTCAGAAC 
TCACGGTACC AGACGTACCA GCGGATGTGG AATTACATGC AGTCGAAGCA GCCCAGCGTG 
TTCGTCAAGA GCACAGAGGA GGGGATCGCC CGTGTCCTCA ACTCACGTTA CGCCTTCCTG 
CTGGAGTCCA CCATGAACGA GTACCACAGG CGCCTCAATT GCAACCTCAC CCAGATCGGG 
GGCCTCCTCG ACACCAAGGG CTACGGCATC GGCATGCCGC TGGGCTCCCC ATTCCGGGAT 
GAGATCACGC TGGCCATCCT GCAGCTCCAG GAGAACAACA GGCTGGAGAT CCTGAAGCGC 
AAGTGGTGGG AGGGCGGCCG GTGCCCCAAG GAGGAGGACC ACAGGGCCAA AGGTTTGGGC 
ATGGAGAACA TTGGCGGTAT TTTTGTCGTG CTGATCTGTG GCCTCATCAT TGCTGTCTTC 
GTGGCGGTCA TGGAATTCAT CTGGTCCACG CGGAGGTCAG CGGAGTCTGA GGAGGTGTCG 
GTGTGCCAGG AGATGCTGCA GGAGCTGCGC CACGCCGTGT CTTGCCGCAA GACCTCGCGT 
TCCCGCCGGC GCCGGCGCCC TGGCGGCCCG AGCCGGGCCC TGCTGTCGCT GCGCGCGGTC 
CGCGAGATGC GACTCAGCAA CGGCAAGCTC TACTCGGCCG GCGCGGGCGG GGACGCGGGC 
GCGCACGGGG GTCCGCAGCG CCTCCTGGAC GACCCCGGGC CTCCTGGGGG ACCCCGGCCC 
CAGGCTCCCA CGCCCTGCAC GCACGTGCGC GTCTGCCAGG AGTGCAGGCG CATCCAGGCG 
CTGCGAGCTT CAGGGGCCGG GGCGCCCCCG CGTGGCCTGG GCACTCCGGC CGAAGCCACC 
AGCCCGCCTC GGCCGCGGCC TGGACCCACT GGACCCCGCG AGCTGACTGA GCATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grik5 ( XM_006228382.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228444.1 Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22386
Clone ID Related Accession (Same CDS sequence)	XM_006228382.3
Accession Version	XM_006228382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, kainate 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCTG AGCTGCTGCT GCTGCTGATA GTCGCCTTCG CCAACCCCAG CTGCCAGGTG 
CTGTCATCAC TGCGCATGGC TGCAATCCTG GACGACCAGA CCGTGTGTGG CCGTGGTGAG 
CGTCTGGCCC TGGCCCTGGC CCGAGAGCAG ATCAATGGGA TCATCGAGGT CCCAGCCAAG 
GCCCGAGTGG AAGTAGACAT CTTTGAGCTG CAACGGGACA GCCAGTACGA GACTACGGAC 
ACCATGTGTC AGATCCTGCC CAAGGGGGTT GTGTCTGTCT TGGGACCCTC CTCCAGCCCA 
GCTTCTGCCT CCACCGTGAG CCATATCTGT GGGGAGAAGG AGATTCCCCA CATCAAGGTA 
GGCCCTGAGG AGACACCCCG CCTTCAGTAC CTCCGCTTTG CATCTGTCAG CCTGTATCCC 
AGTAATGAAG ATGTCAGCCT GGCAGTCTCC CGGATCCTCA AGTCCTTTAA CTACCCCTCC 
GCTAGCCTCA TCTGCGCCAA AGCTGAGTGC CTGCTGCGGT TAGAAGAACT GGTGCGAGGC 
TTCCTCATCT CCAAGGAGAC ACTGTCCGTG AGGATGCTTG ATGACAGCCG GGACCCCACG 
CCGCTACTCA AGGAGATCCG AGATGACAAA GTGTCCACCA TCATCATCGA TGCCAACGCG 
TCCATCTCCC ACCTTGTCCT CCGTAAGGCT TCGGAGCTGG GAATGACCTC AGCGTTTTAC 
AAGTACATCC TCACCACCAT GGACTTCCCC ATCCTGCATC TGGACGGTAT CGTAGAGGAC 
TCCTCCAACA TCTTGGGCTT TTCCATGTTC AACACCTCCC ATCCCTTCTA CCCGGAGTTT 
GTGCGCAGCC TCAACATGTC CTGGAGGGAG AACTGCGAAG CCAGCACCTA CCCTGGCCCT 
GCGCTGTCGG CAGCCCTGAT GTTTGATGCC GTGCATGTGG TGGTAAGCGC TGTCCGAGAG 
CTGAACCGGA GCCAGGAGAT TGGTGTCAAG CCACTGGCTT GCACTTCGGC CAACATCTGG 
CCCCATGGGA CCAGCCTCAT GAACTACCTG CGCATGGTAG AGTATGACGG GCTGACTGGG 
CGGGTTGAGT TCAACAGCAA AGGGCAGAGG ACCAACTACA CACTGCGCAT CCTGGAGAAG 
TCCCGCCAGG GCCACCGTGA GATAGGGGTG TGGTACTCTA ACCGGACCCT GGCCATGAAT 
GCTACCACCC TGGACATCAA CCTGTCACAG ACTCTAGCCA ACAAGACTCT GGTGGTCACA 
ACAATCCTGG AGAACCCGTA TGTCATGCGC CGGCCCAACT TCCAGGCCCT GTCAGGGAAC 
GAGCGCTTTG AGGGCTTCTG CGTGGACATG CTTCGGGAGC TGGCCGAGCT GCTGCGCTTC 
CGGTATCGCC TGCGGTTGGT GGAGGACGGA CTGTACGGGG CACCTGAGCC CAATGGTTCC 
TGGACAGGAA TGGTTGGCGA GCTCATCAAC CGGCAGAAGG CAGACCTGGC TGTGGCAGCC 
TTCACCATCA CCGCGGAGAG AGAGAAGGTC ATCGACTTCT CCAAGCCATT CATGACCCTG 
GGGATCAGCA TCCTCTACAG GGTGCACATG GGCCGCAAGC CTGGCTACTT CTCCTTCCTG 
GACCCTTTCT CCCCTGCCGT GTGGCTCTTC ATGCTTCTTG CCTACCTGGC TGTCAGCTGT 
GTCTTGTTTC TGGCTGCCAG GCTGAGCCCT TATGAGTGGT ATAACCCTCA CCCGTGTCTT 
CGGGCGCGTC CCCATATCCT GGAGAACCAG TACACGCTGG GTAACAGCCT TTGGTTCCCT 
GTGGGTGGCT TCATGCAGCA GGGCTCGGAG ATCATGCCCC GGGCGCTGTC CACACGCTGT 
GTCAGCGGAG TCTGGTGGGC CTTCACCTTG ATCATCATCT CCTCCTACAC GGCCAACCTG 
GCTGCCTTCC TCACGGTGCA GCGCATGGAG GTGCCGGTGG AGTCTGCAGA CGACTTGGCG 
GATCAGACCA ACATTGAGTA CGGCACTATC CACGCTGGCT CCACCATGAC CTTCTTTCAG 
AACTCACGGT ACCAGACGTA CCAGCGGATG TGGAATTACA TGCAGTCGAA GCAGCCCAGC 
GTGTTCGTCA AGAGCACAGA GGAGGGGATC GCCCGTGTCC TCAACTCACG TTACGCCTTC 
CTGCTGGAGT CCACCATGAA CGAGTACCAC AGGCGCCTCA ATTGCAACCT CACCCAGATC 
GGGGGCCTCC TCGACACCAA GGGCTACGGC ATCGGCATGC CGCTGGGCTC CCCATTCCGG 
GATGAGATCA CGCTGGCCAT CCTGCAGCTC CAGGAGAACA ACAGGCTGGA GATCCTGAAG 
CGCAAGTGGT GGGAGGGCGG CCGGTGCCCC AAGGAGGAGG ACCACAGGGC CAAAGGTTTG 
GGCATGGAGA ACATTGGCGG TATTTTTGTC GTGCTGATCT GTGGCCTCAT CATTGCTGTC 
TTCGTGGCGG TCATGGAATT CATCTGGTCC ACGCGGAGGT CAGCGGAGTC TGAGGAGGTG 
TCGGTGTGCC AGGAGATGCT GCAGGAGCTG CGCCACGCCG TGTCTTGCCG CAAGACCTCG 
CGTTCCCGCC GGCGCCGGCG CCCTGGCGGC CCGAGCCGGG CCCTGCTGTC GCTGCGCGCG 
GTCCGCGAGA TGCGACTCAG CAACGGCAAG CTCTACTCGG CCGGCGCGGG CGGGGACGCG 
GGCGCGCACG GGGGTCCGCA GCGCCTCCTG GACGACCCCG GGCCTCCTGG GGGACCCCGG 
CCCCAGGCTC CCACGCCCTG CACGCACGTG CGCGTCTGCC AGGAGTGCAG GCGCATCCAG 
GCGCTGCGAG CTTCAGGGGC CGGGGCGCCC CCGCGTGGCC TGGGCACTCC GGCCGAAGCC 
ACCAGCCCGC CTCGGCCGCG GCCTGGACCC ACTGGACCCC GCGAGCTGAC TGAGCATGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228444.1
CDS	322..3264
Translation	MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIRDDKVSTIIIDANASISHLVLRKASELGMTSAFYKYILTTMDFPILHLDGIVEDSSNILGFSMFNTSHPFYPEFVRSLNMSWRENCEASTYPGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYLRMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVTTILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTGMVGELINRQKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSVFVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRKWWEGGRCPKEEDHRAKGLGMENIGGIFVVLICGLIIAVFVAVMEFIWSTRRSAESEEVSVCQEMLQELRHAVSCRKTSRSRRRRRPGGPSRALLSLRAVREMRLSNGKLYSAGAGGDAGAHGGPQRLLDDPGPPGGPRPQAPTPCTHVRVCQECRRIQALRASGAGAPPRGLGTPAEATSPPRPRPGPTGPRELTEHE

Target ORF information:

RefSeq Version	XM_006228382.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228382.3

ORF Insert Sequence:

ATGCCGGCTG AGCTGCTGCT GCTGCTGATA GTCGCCTTCG CCAACCCCAG CTGCCAGGTG 
CTGTCATCAC TGCGCATGGC TGCAATCCTG GACGACCAGA CCGTGTGTGG CCGTGGTGAG 
CGTCTGGCCC TGGCCCTGGC CCGAGAGCAG ATCAATGGGA TCATCGAGGT CCCAGCCAAG 
GCCCGAGTGG AAGTAGACAT CTTTGAGCTG CAACGGGACA GCCAGTACGA GACTACGGAC 
ACCATGTGTC AGATCCTGCC CAAGGGGGTT GTGTCTGTCT TGGGACCCTC CTCCAGCCCA 
GCTTCTGCCT CCACCGTGAG CCATATCTGT GGGGAGAAGG AGATTCCCCA CATCAAGGTA 
GGCCCTGAGG AGACACCCCG CCTTCAGTAC CTCCGCTTTG CATCTGTCAG CCTGTATCCC 
AGTAATGAAG ATGTCAGCCT GGCAGTCTCC CGGATCCTCA AGTCCTTTAA CTACCCCTCC 
GCTAGCCTCA TCTGCGCCAA AGCTGAGTGC CTGCTGCGGT TAGAAGAACT GGTGCGAGGC 
TTCCTCATCT CCAAGGAGAC ACTGTCCGTG AGGATGCTTG ATGACAGCCG GGACCCCACG 
CCGCTACTCA AGGAGATCCG AGATGACAAA GTGTCCACCA TCATCATCGA TGCCAACGCG 
TCCATCTCCC ACCTTGTCCT CCGTAAGGCT TCGGAGCTGG GAATGACCTC AGCGTTTTAC 
AAGTACATCC TCACCACCAT GGACTTCCCC ATCCTGCATC TGGACGGTAT CGTAGAGGAC 
TCCTCCAACA TCTTGGGCTT TTCCATGTTC AACACCTCCC ATCCCTTCTA CCCGGAGTTT 
GTGCGCAGCC TCAACATGTC CTGGAGGGAG AACTGCGAAG CCAGCACCTA CCCTGGCCCT 
GCGCTGTCGG CAGCCCTGAT GTTTGATGCC GTGCATGTGG TGGTAAGCGC TGTCCGAGAG 
CTGAACCGGA GCCAGGAGAT TGGTGTCAAG CCACTGGCTT GCACTTCGGC CAACATCTGG 
CCCCATGGGA CCAGCCTCAT GAACTACCTG CGCATGGTAG AGTATGACGG GCTGACTGGG 
CGGGTTGAGT TCAACAGCAA AGGGCAGAGG ACCAACTACA CACTGCGCAT CCTGGAGAAG 
TCCCGCCAGG GCCACCGTGA GATAGGGGTG TGGTACTCTA ACCGGACCCT GGCCATGAAT 
GCTACCACCC TGGACATCAA CCTGTCACAG ACTCTAGCCA ACAAGACTCT GGTGGTCACA 
ACAATCCTGG AGAACCCGTA TGTCATGCGC CGGCCCAACT TCCAGGCCCT GTCAGGGAAC 
GAGCGCTTTG AGGGCTTCTG CGTGGACATG CTTCGGGAGC TGGCCGAGCT GCTGCGCTTC 
CGGTATCGCC TGCGGTTGGT GGAGGACGGA CTGTACGGGG CACCTGAGCC CAATGGTTCC 
TGGACAGGAA TGGTTGGCGA GCTCATCAAC CGGCAGAAGG CAGACCTGGC TGTGGCAGCC 
TTCACCATCA CCGCGGAGAG AGAGAAGGTC ATCGACTTCT CCAAGCCATT CATGACCCTG 
GGGATCAGCA TCCTCTACAG GGTGCACATG GGCCGCAAGC CTGGCTACTT CTCCTTCCTG 
GACCCTTTCT CCCCTGCCGT GTGGCTCTTC ATGCTTCTTG CCTACCTGGC TGTCAGCTGT 
GTCTTGTTTC TGGCTGCCAG GCTGAGCCCT TATGAGTGGT ATAACCCTCA CCCGTGTCTT 
CGGGCGCGTC CCCATATCCT GGAGAACCAG TACACGCTGG GTAACAGCCT TTGGTTCCCT 
GTGGGTGGCT TCATGCAGCA GGGCTCGGAG ATCATGCCCC GGGCGCTGTC CACACGCTGT 
GTCAGCGGAG TCTGGTGGGC CTTCACCTTG ATCATCATCT CCTCCTACAC GGCCAACCTG 
GCTGCCTTCC TCACGGTGCA GCGCATGGAG GTGCCGGTGG AGTCTGCAGA CGACTTGGCG 
GATCAGACCA ACATTGAGTA CGGCACTATC CACGCTGGCT CCACCATGAC CTTCTTTCAG 
AACTCACGGT ACCAGACGTA CCAGCGGATG TGGAATTACA TGCAGTCGAA GCAGCCCAGC 
GTGTTCGTCA AGAGCACAGA GGAGGGGATC GCCCGTGTCC TCAACTCACG TTACGCCTTC 
CTGCTGGAGT CCACCATGAA CGAGTACCAC AGGCGCCTCA ATTGCAACCT CACCCAGATC 
GGGGGCCTCC TCGACACCAA GGGCTACGGC ATCGGCATGC CGCTGGGCTC CCCATTCCGG 
GATGAGATCA CGCTGGCCAT CCTGCAGCTC CAGGAGAACA ACAGGCTGGA GATCCTGAAG 
CGCAAGTGGT GGGAGGGCGG CCGGTGCCCC AAGGAGGAGG ACCACAGGGC CAAAGGTTTG 
GGCATGGAGA ACATTGGCGG TATTTTTGTC GTGCTGATCT GTGGCCTCAT CATTGCTGTC 
TTCGTGGCGG TCATGGAATT CATCTGGTCC ACGCGGAGGT CAGCGGAGTC TGAGGAGGTG 
TCGGTGTGCC AGGAGATGCT GCAGGAGCTG CGCCACGCCG TGTCTTGCCG CAAGACCTCG 
CGTTCCCGCC GGCGCCGGCG CCCTGGCGGC CCGAGCCGGG CCCTGCTGTC GCTGCGCGCG 
GTCCGCGAGA TGCGACTCAG CAACGGCAAG CTCTACTCGG CCGGCGCGGG CGGGGACGCG 
GGCGCGCACG GGGGTCCGCA GCGCCTCCTG GACGACCCCG GGCCTCCTGG GGGACCCCGG 
CCCCAGGCTC CCACGCCCTG CACGCACGTG CGCGTCTGCC AGGAGTGCAG GCGCATCCAG 
GCGCTGCGAG CTTCAGGGGC CGGGGCGCCC CCGCGTGGCC TGGGCACTCC GGCCGAAGCC 
ACCAGCCCGC CTCGGCCGCG GCCTGGACCC ACTGGACCCC GCGAGCTGAC TGAGCATGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Grik5 ( XM_008758889.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757111.1 Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), transcript variant X2, mRNA.

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CloneID	ORa42907
Clone ID Related Accession (Same CDS sequence)	XM_008758889.2
Accession Version	XM_008758889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate receptor ionotropic, kainate 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008758889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCTG AGCTGCTGCT GCTGCTGATA GTCGCCTTCG CCAACCCCAG CTGCCAGGTG 
CTGTCATCAC TGCGCATGGC TGCAATCCTG GACGACCAGA CCGTGTGTGG CCGTGGTGAG 
CGTCTGGCCC TGGCCCTGGC CCGAGAGCAG ATCAATGGGA TCATCGAGGT CCCAGCCAAG 
GCCCGAGTGG AAGTAGACAT CTTTGAGCTG CAACGGGACA GCCAGTACGA GACTACGGAC 
ACCATGTGTC AGATCCTGCC CAAGGGGGTT GTGTCTGTCT TGGGACCCTC CTCCAGCCCA 
GCTTCTGCCT CCACCGTGAG CCATATCTGT GGGGAGAAGG AGATTCCCCA CATCAAGGTA 
GGCCCTGAGG AGACACCCCG CCTTCAGTAC CTCCGCTTTG CATCTGTCAG CCTGTATCCC 
AGTAATGAAG ATGTCAGCCT GGCAGTCTCC CGGATCCTCA AGTCCTTTAA CTACCCCTCC 
GCTAGCCTCA TCTGCGCCAA AGCTGAGTGC CTGCTGCGGT TAGAAGAACT GGTGCGAGGC 
TTCCTCATCT CCAAGGAGAC ACTGTCCGTG AGGATGCTTG ATGACAGCCG GGACCCCACG 
CCGCTACTCA AGGAGATCCG AGATGACAAA GTGTCCACCA TCATCATCGA TGCCAACGCG 
TCCATCTCCC ACCTTGTCCT CCGTAAGGAC TTCCCCATCC TGCATCTGGA CGGTATCGTA 
GAGGACTCCT CCAACATCTT GGGCTTTTCC ATGTTCAACA CCTCCCATCC CTTCTACCCG 
GAGTTTGTGC GCAGCCTCAA CATGTCCTGG AGGGAGAACT GCGAAGCCAG CACCTACCCT 
GGCCCTGCGC TGTCGGCAGC CCTGATGTTT GATGCCGTGC ATGTGGTGGT AAGCGCTGTC 
CGAGAGCTGA ACCGGAGCCA GGAGATTGGT GTCAAGCCAC TGGCTTGCAC TTCGGCCAAC 
ATCTGGCCCC ATGGGACCAG CCTCATGAAC TACCTGCGCA TGGTAGAGTA TGACGGGCTG 
ACTGGGCGGG TTGAGTTCAA CAGCAAAGGG CAGAGGACCA ACTACACACT GCGCATCCTG 
GAGAAGTCCC GCCAGGGCCA CCGTGAGATA GGGGTGTGGT ACTCTAACCG GACCCTGGCC 
ATGAATGCTA CCACCCTGGA CATCAACCTG TCACAGACTC TAGCCAACAA GACTCTGGTG 
GTCACAACAA TCCTGGAGAA CCCGTATGTC ATGCGCCGGC CCAACTTCCA GGCCCTGTCA 
GGGAACGAGC GCTTTGAGGG CTTCTGCGTG GACATGCTTC GGGAGCTGGC CGAGCTGCTG 
CGCTTCCGGT ATCGCCTGCG GTTGGTGGAG GACGGACTGT ACGGGGCACC TGAGCCCAAT 
GGTTCCTGGA CAGGAATGGT TGGCGAGCTC ATCAACCGGC AGAAGGCAGA CCTGGCTGTG 
GCAGCCTTCA CCATCACCGC GGAGAGAGAG AAGGTCATCG ACTTCTCCAA GCCATTCATG 
ACCCTGGGGA TCAGCATCCT CTACAGGGTG CACATGGGCC GCAAGCCTGG CTACTTCTCC 
TTCCTGGACC CTTTCTCCCC TGCCGTGTGG CTCTTCATGC TTCTTGCCTA CCTGGCTGTC 
AGCTGTGTCT TGTTTCTGGC TGCCAGGCTG AGCCCTTATG AGTGGTATAA CCCTCACCCG 
TGTCTTCGGG CGCGTCCCCA TATCCTGGAG AACCAGTACA CGCTGGGTAA CAGCCTTTGG 
TTCCCTGTGG GTGGCTTCAT GCAGCAGGGC TCGGAGATCA TGCCCCGGGC GCTGTCCACA 
CGCTGTGTCA GCGGAGTCTG GTGGGCCTTC ACCTTGATCA TCATCTCCTC CTACACGGCC 
AACCTGGCTG CCTTCCTCAC GGTGCAGCGC ATGGAGGTGC CGGTGGAGTC TGCAGACGAC 
TTGGCGGATC AGACCAACAT TGAGTACGGC ACTATCCACG CTGGCTCCAC CATGACCTTC 
TTTCAGAACT CACGGTACCA GACGTACCAG CGGATGTGGA ATTACATGCA GTCGAAGCAG 
CCCAGCGTGT TCGTCAAGAG CACAGAGGAG GGGATCGCCC GTGTCCTCAA CTCACGTTAC 
GCCTTCCTGC TGGAGTCCAC CATGAACGAG TACCACAGGC GCCTCAATTG CAACCTCACC 
CAGATCGGGG GCCTCCTCGA CACCAAGGGC TACGGCATCG GCATGCCGCT GGGCTCCCCA 
TTCCGGGATG AGATCACGCT GGCCATCCTG CAGCTCCAGG AGAACAACAG GCTGGAGATC 
CTGAAGCGCA AGTGGTGGGA GGGCGGCCGG TGCCCCAAGG AGGAGGACCA CAGGGCCAAA 
GGTTTGGGCA TGGAGAACAT TGGCGGTATT TTTGTCGTGC TGATCTGTGG CCTCATCATT 
GCTGTCTTCG TGGCGGTCAT GGAATTCATC TGGTCCACGC GGAGGTCAGC GGAGTCTGAG 
GAGGTGTCGG TGTGCCAGGA GATGCTGCAG GAGCTGCGCC ACGCCGTGTC TTGCCGCAAG 
ACCTCGCGTT CCCGCCGGCG CCGGCGCCCT GGCGGCCCGA GCCGGGCCCT GCTGTCGCTG 
CGCGCGGTCC GCGAGATGCG ACTCAGCAAC GGCAAGCTCT ACTCGGCCGG CGCGGGCGGG 
GACGCGGGCG CGCACGGGGG TCCGCAGCGC CTCCTGGACG ACCCCGGGCC TCCTGGGGGA 
CCCCGGCCCC AGGCTCCCAC GCCCTGCACG CACGTGCGCG TCTGCCAGGA GTGCAGGCGC 
ATCCAGGCGC TGCGAGCTTC AGGGGCCGGG GCGCCCCCGC GTGGCCTGGG CACTCCGGCC 
GAAGCCACCA GCCCGCCTCG GCCGCGGCCT GGACCCACTG GACCCCGCGA GCTGACTGAG 
CATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757111.1
CDS	322..3210
Translation	MPAELLLLLIVAFANPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIRDDKVSTIIIDANASISHLVLRKDFPILHLDGIVEDSSNILGFSMFNTSHPFYPEFVRSLNMSWRENCEASTYPGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYLRMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVTTILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTGMVGELINRQKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSVFVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRKWWEGGRCPKEEDHRAKGLGMENIGGIFVVLICGLIIAVFVAVMEFIWSTRRSAESEEVSVCQEMLQELRHAVSCRKTSRSRRRRRPGGPSRALLSLRAVREMRLSNGKLYSAGAGGDAGAHGGPQRLLDDPGPPGGPRPQAPTPCTHVRVCQECRRIQALRASGAGAPPRGLGTPAEATSPPRPRPGPTGPRELTEHE

Target ORF information:

RefSeq Version	XM_008758889.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate ionotropic receptor kainate type subunit 5 (Grik5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758889.2

ORF Insert Sequence:

ATGCCGGCTG AGCTGCTGCT GCTGCTGATA GTCGCCTTCG CCAACCCCAG CTGCCAGGTG 
CTGTCATCAC TGCGCATGGC TGCAATCCTG GACGACCAGA CCGTGTGTGG CCGTGGTGAG 
CGTCTGGCCC TGGCCCTGGC CCGAGAGCAG ATCAATGGGA TCATCGAGGT CCCAGCCAAG 
GCCCGAGTGG AAGTAGACAT CTTTGAGCTG CAACGGGACA GCCAGTACGA GACTACGGAC 
ACCATGTGTC AGATCCTGCC CAAGGGGGTT GTGTCTGTCT TGGGACCCTC CTCCAGCCCA 
GCTTCTGCCT CCACCGTGAG CCATATCTGT GGGGAGAAGG AGATTCCCCA CATCAAGGTA 
GGCCCTGAGG AGACACCCCG CCTTCAGTAC CTCCGCTTTG CATCTGTCAG CCTGTATCCC 
AGTAATGAAG ATGTCAGCCT GGCAGTCTCC CGGATCCTCA AGTCCTTTAA CTACCCCTCC 
GCTAGCCTCA TCTGCGCCAA AGCTGAGTGC CTGCTGCGGT TAGAAGAACT GGTGCGAGGC 
TTCCTCATCT CCAAGGAGAC ACTGTCCGTG AGGATGCTTG ATGACAGCCG GGACCCCACG 
CCGCTACTCA AGGAGATCCG AGATGACAAA GTGTCCACCA TCATCATCGA TGCCAACGCG 
TCCATCTCCC ACCTTGTCCT CCGTAAGGAC TTCCCCATCC TGCATCTGGA CGGTATCGTA 
GAGGACTCCT CCAACATCTT GGGCTTTTCC ATGTTCAACA CCTCCCATCC CTTCTACCCG 
GAGTTTGTGC GCAGCCTCAA CATGTCCTGG AGGGAGAACT GCGAAGCCAG CACCTACCCT 
GGCCCTGCGC TGTCGGCAGC CCTGATGTTT GATGCCGTGC ATGTGGTGGT AAGCGCTGTC 
CGAGAGCTGA ACCGGAGCCA GGAGATTGGT GTCAAGCCAC TGGCTTGCAC TTCGGCCAAC 
ATCTGGCCCC ATGGGACCAG CCTCATGAAC TACCTGCGCA TGGTAGAGTA TGACGGGCTG 
ACTGGGCGGG TTGAGTTCAA CAGCAAAGGG CAGAGGACCA ACTACACACT GCGCATCCTG 
GAGAAGTCCC GCCAGGGCCA CCGTGAGATA GGGGTGTGGT ACTCTAACCG GACCCTGGCC 
ATGAATGCTA CCACCCTGGA CATCAACCTG TCACAGACTC TAGCCAACAA GACTCTGGTG 
GTCACAACAA TCCTGGAGAA CCCGTATGTC ATGCGCCGGC CCAACTTCCA GGCCCTGTCA 
GGGAACGAGC GCTTTGAGGG CTTCTGCGTG GACATGCTTC GGGAGCTGGC CGAGCTGCTG 
CGCTTCCGGT ATCGCCTGCG GTTGGTGGAG GACGGACTGT ACGGGGCACC TGAGCCCAAT 
GGTTCCTGGA CAGGAATGGT TGGCGAGCTC ATCAACCGGC AGAAGGCAGA CCTGGCTGTG 
GCAGCCTTCA CCATCACCGC GGAGAGAGAG AAGGTCATCG ACTTCTCCAA GCCATTCATG 
ACCCTGGGGA TCAGCATCCT CTACAGGGTG CACATGGGCC GCAAGCCTGG CTACTTCTCC 
TTCCTGGACC CTTTCTCCCC TGCCGTGTGG CTCTTCATGC TTCTTGCCTA CCTGGCTGTC 
AGCTGTGTCT TGTTTCTGGC TGCCAGGCTG AGCCCTTATG AGTGGTATAA CCCTCACCCG 
TGTCTTCGGG CGCGTCCCCA TATCCTGGAG AACCAGTACA CGCTGGGTAA CAGCCTTTGG 
TTCCCTGTGG GTGGCTTCAT GCAGCAGGGC TCGGAGATCA TGCCCCGGGC GCTGTCCACA 
CGCTGTGTCA GCGGAGTCTG GTGGGCCTTC ACCTTGATCA TCATCTCCTC CTACACGGCC 
AACCTGGCTG CCTTCCTCAC GGTGCAGCGC ATGGAGGTGC CGGTGGAGTC TGCAGACGAC 
TTGGCGGATC AGACCAACAT TGAGTACGGC ACTATCCACG CTGGCTCCAC CATGACCTTC 
TTTCAGAACT CACGGTACCA GACGTACCAG CGGATGTGGA ATTACATGCA GTCGAAGCAG 
CCCAGCGTGT TCGTCAAGAG CACAGAGGAG GGGATCGCCC GTGTCCTCAA CTCACGTTAC 
GCCTTCCTGC TGGAGTCCAC CATGAACGAG TACCACAGGC GCCTCAATTG CAACCTCACC 
CAGATCGGGG GCCTCCTCGA CACCAAGGGC TACGGCATCG GCATGCCGCT GGGCTCCCCA 
TTCCGGGATG AGATCACGCT GGCCATCCTG CAGCTCCAGG AGAACAACAG GCTGGAGATC 
CTGAAGCGCA AGTGGTGGGA GGGCGGCCGG TGCCCCAAGG AGGAGGACCA CAGGGCCAAA 
GGTTTGGGCA TGGAGAACAT TGGCGGTATT TTTGTCGTGC TGATCTGTGG CCTCATCATT 
GCTGTCTTCG TGGCGGTCAT GGAATTCATC TGGTCCACGC GGAGGTCAGC GGAGTCTGAG 
GAGGTGTCGG TGTGCCAGGA GATGCTGCAG GAGCTGCGCC ACGCCGTGTC TTGCCGCAAG 
ACCTCGCGTT CCCGCCGGCG CCGGCGCCCT GGCGGCCCGA GCCGGGCCCT GCTGTCGCTG 
CGCGCGGTCC GCGAGATGCG ACTCAGCAAC GGCAAGCTCT ACTCGGCCGG CGCGGGCGGG 
GACGCGGGCG CGCACGGGGG TCCGCAGCGC CTCCTGGACG ACCCCGGGCC TCCTGGGGGA 
CCCCGGCCCC AGGCTCCCAC GCCCTGCACG CACGTGCGCG TCTGCCAGGA GTGCAGGCGC 
ATCCAGGCGC TGCGAGCTTC AGGGGCCGGG GCGCCCCCGC GTGGCCTGGG CACTCCGGCC 
GAAGCCACCA GCCCGCCTCG GCCGCGGCCT GGACCCACTG GACCCCGCGA GCTGACTGAG 
CATGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Agonist binding to the GluK5 subunit is sufficient for functional surface expression of heteromeric GluK2/GluK5 kainate receptors.
Cellular and molecular neurobiology33(8)1099-108(2013 Nov)
Fisher JL,Housley PR

Selective block of postsynaptic kainate receptors reveals their function at hippocampal mossy fiber synapses.
Cerebral cortex (New York, N.Y. : 1991)23(2)323-31(2013 Feb)
Pinheiro PS,Lanore F,Veran J,Artinian J,Blanchet C,Cr?pel V,Perrais D,Mulle C

CaMKII-dependent phosphorylation of GluK5 mediates plasticity of kainate receptors.
The EMBO journal32(4)496-510(2013 Feb)
Carta M,Opazo P,Veran J,Athan? A,Choquet D,Coussen F,Mulle C

Assembly stoichiometry of the GluK2/GluK5 kainate receptor complex.
Cell reports1(3)234-40(2012 Mar)
Reiner A,Arant RJ,Isacoff EY

Distinct functional roles of subunits within the heteromeric kainate receptor.
The Journal of neuroscience : the official journal of the Society for Neuroscience31(47)17113-22(2011 Nov)
Fisher JL,Mott DD

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Grik5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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