Gpx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpx1
Entrez Gene ID	24404
Full Name	glutathione peroxidase 1
Synonyms	GSHPx,GSHPx-1
General protein information	Preferred Names glutathione peroxidase 1 Names glutathione peroxidase 1 GPx-1 cellular glutathione peroxidase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32
Summary	The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide. It is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_030826.4	NP_110453.3	glutathione peroxidase 1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	gpx1a	NP_001007282.2
Homo sapiens (human)	GPX1	NP_000572.2
Pan troglodytes (chimpanzee)	GPX1	NP_001070980.2
Canis lupus familiaris (dog)	GPX1	NP_001108591.1
Bos taurus (cattle)	GPX1	NP_776501.1
Danio rerio (zebrafish)	gpx1b	NP_001004634.2
Xenopus tropicalis (tropical clawed frog)	gpx1	NP_001015740.2
Mus musculus (house mouse)	Gpx1	NP_032186.2
Rattus norvegicus (Norway rat)	Gpx1	NP_110453.3
Gallus gallus (chicken)	GPX1	NP_001264782.1
	GPX1	NP_001152770.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of AAV-mediated knockdown of nNOS and GPx-1 gene expression in rat hippocampus after traumatic brain injury.
Boone DR, Leek JM, Falduto MT, Torres KEO, Sell SL, Parsley MA, Cowart JC, Uchida T, Micci MA, DeWitt DS, Prough DS, Hellmich HL
PloS one12(10)e0185943(2017)

Genetic variants involved in oxidative stress, base excision repair, DNA methylation, and folate metabolism pathways influence myeloid neoplasias susceptibility and prognosis.
Gon?alves AC, Alves R, Baldeiras I, Cortes?o E, Carda JP, Branco CC, Oliveiros B, Loureiro L, Pereira A, Nascimento Costa JM, Sarmento-Ribeiro AB, Mota-Vieira L
Molecular carcinogenesis56(1)130-148(2017 Jan)

Among a panel of polymorphisms in genes related to oxidative stress, CAT-262 C>T, GPX1 Pro198Leu and GSTP1 Ile105Val influence the risk of developing BCR-ABL negative myeloproliferative neoplasms.
Trifa AP, B?nescu C, Dima D, Bojan AS, Tevet M, Moldovan VG, Vesa ?C, Murat M, Pop IV, Skrypnyk C, Popp RA
Hematology (Amsterdam, Netherlands)21(9)520-5(2016 Oct)

From Six Gene Polymorphisms of the Antioxidant System, Only GPX Pro198Leu and GSTP1 Ile105Val Modulate the Risk of Acute Myeloid Leukemia.
B?nescu C, Iancu M, Trifa AP, C?ndea M, Benedek Lazar E, Moldovan VG, Duicu C, Tripon F, Crauciuc A, Dobreanu M
Oxidative medicine and cellular longevity20162536705(2016)

Determination of nucleotide sequence of cDNA coding rat glutathione peroxidase and diminished expression of the mRNA in selenium deficient rat liver.
Yoshimura S, Takekoshi S, Watanabe K, Fujii-Kuriyama Y
Biochemical and biophysical research communications154(3)1024-8(1988 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of nNOS or GPx1 was specific and had broad effects on downstream genes, though it only modestly ameliorated traumatic brain injury-induced working memory deficits, despite widespread genomic changes.
Title: Effects of AAV-mediated knockdown of nNOS and GPx-1 gene expression in rat hippocampus after traumatic brain injury.

VPO1 plays an important role in senescence of endothelial cells and endothelial dysfunction by induction of oxidative stress and inflammatory reaction in type 2 diabetic rats.
Title: Role of vascular peroxidase 1 in senescence of endothelial cells in diabetes rats.

glutathione peroxidase 1 immunoreactivity was diffusely cytoplasmic in some of the hepatocytes
Title: Distribution of glutathione peroxidase 1 in liver tissues of healthy and diabetic rats treated with capsaisin.

Decrease in Sirtuin1 (SIRT1) and nuclear factor erythroid 2-related factor (Nrf2) and increase in nuclear factor kappa B (NFkappaB) gene expression in diabetes were associated with a decrease in CAT and GPx mRNA expression.
Title: Redox regulation of antioxidant enzymes: post-translational modulation of catalase and glutathione peroxidase activity by resveratrol in diabetic rat liver.

Dietary Se-enriched A. bisporus protected against increase in ileum permeability and up-regulated GPx-1 and GPx-2 expression, selenoenzymes relevant to mitigating oxidative stress.
Title: Selenium-enriched Agaricus bisporus mushroom protects against increase in gut permeability ex vivo and up-regulates glutathione peroxidase 1 and 2 in hyperthermally-induced oxidative stress in rats.

The following Gpx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12505	NM_030826.4 Latest version!	Rattus norvegicus glutathione peroxidase 1 (Gpx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpx1 ( NM_030826.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_110453.3 Rattus norvegicus glutathione peroxidase 1 (Gpx1), mRNA.

Price & Availability↑

CloneID	ORa12505
Clone ID Related Accession (Same CDS sequence)	NM_030826.4
Accession Version	NM_030826.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	glutathione peroxidase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FQ139913.1 and FQ231953.1. On Jul 15, 2016 this sequence version replaced NM_030826.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ227523.1, FQ211052.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 3408482 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGCTG CTCGGCTCTC CGCGGTGGCA CAGTCCACCG TGTATGCCTT CTCCGCGCGC 
CCGCTGGCGG GCGGGGAGCC CGTGAGCCTG GGCTCCCTGC GGGGCAAGGT GCTGCTCATT 
GAGAATGTCG CGTCCCTCTG AGGCACCACG ACCCGGGACT ACACCGAAAT GAATGATCTG 
CAGAAGCGTC TGGGGCCTCG TGGCCTGGTG GTGCTCGGTT TCCCGTGCAA TCAGTTCGGA 
CATCAGGAGA ATGGCAAGAA TGAAGAGATT CTGAATTCCC TCAAGTATGT CCGACCCGGT 
GGTGGGTTCG AGCCCAACTT TACATTGTTT GAGAAGTGCG AGGTGAATGG TGAGAAGGCT 
CACCCGCTCT TTACCTTCCT GCGGAATGCC TTGCCAGCAC CCAGTGACGA TCCCACTGCG 
CTCATGACCG ACCCCAAGTA CATCATTTGG TCCCCGGTGT GCCGCAACGA CATTTCCTGG 
AACTTTGAGA AGTTCCTGGT AGGTCCAGAC GGTGTTCCAG TGCGCAGATA CAGCAGGCGC 
TTTCGCACCA TCGACATCGA ACCCGATATA GAAGCCCTGC TGTCCAAGCA GCCTAGCAAC 
CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110453.3
CDS	94..699
Translation	MSAARLSAVAQSTVYAFSARPLAGGEPVSLGSLRGKVLLIENVASL*GTTTRDYTEMNDLQKRLGPRGLVVLGFPCNQFGHQENGKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGEKAHPLFTFLRNALPAPSDDPTALMTDPKYIIWSPVCRNDISWNFEKFLVGPDGVPVRRYSRRFRTIDIEPDIEALLSKQPSNP

Target ORF information:

RefSeq Version	NM_030826.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutathione peroxidase 1 (Gpx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030826.4

ORF Insert Sequence:

ATGTCTGCTG CTCGGCTCTC CGCGGTGGCA CAGTCCACCG TGTATGCCTT CTCCGCGCGC 
CCGCTGGCGG GCGGGGAGCC CGTGAGCCTG GGCTCCCTGC GGGGCAAGGT GCTGCTCATT 
GAGAATGTCG CGTCCCTCTG AGGCACCACG ACCCGGGACT ACACCGAAAT GAATGATCTG 
CAGAAGCGTC TGGGGCCTCG TGGCCTGGTG GTGCTCGGTT TCCCGTGCAA TCAGTTCGGA 
CATCAGGAGA ATGGCAAGAA TGAAGAGATT CTGAATTCCC TCAAGTATGT CCGACCCGGT 
GGTGGGTTCG AGCCCAACTT TACATTGTTT GAGAAGTGCG AGGTGAATGG TGAGAAGGCT 
CACCCGCTCT TTACCTTCCT GCGGAATGCC TTGCCAGCAC CCAGTGACGA TCCCACTGCG 
CTCATGACCG ACCCCAAGTA CATCATTTGG TCCCCGGTGT GCCGCAACGA CATTTCCTGG 
AACTTTGAGA AGTTCCTGGT AGGTCCAGAC GGTGTTCCAG TGCGCAGATA CAGCAGGCGC 
TTTCGCACCA TCGACATCGA ACCCGATATA GAAGCCCTGC TGTCCAAGCA GCCTAGCAAC 
CCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of AAV-mediated knockdown of nNOS and GPx-1 gene expression in rat hippocampus after traumatic brain injury.
PloS one12(10)e0185943(2017)
Boone DR,Leek JM,Falduto MT,Torres KEO,Sell SL,Parsley MA,Cowart JC,Uchida T,Micci MA,DeWitt DS,Prough DS,Hellmich HL

Genetic variants involved in oxidative stress, base excision repair, DNA methylation, and folate metabolism pathways influence myeloid neoplasias susceptibility and prognosis.
Molecular carcinogenesis56(1)130-148(2017 Jan)
Gon?alves AC,Alves R,Baldeiras I,Cortes?o E,Carda JP,Branco CC,Oliveiros B,Loureiro L,Pereira A,Nascimento Costa JM,Sarmento-Ribeiro AB,Mota-Vieira L

Among a panel of polymorphisms in genes related to oxidative stress, CAT-262 C>T, GPX1 Pro198Leu and GSTP1 Ile105Val influence the risk of developing BCR-ABL negative myeloproliferative neoplasms.
Hematology (Amsterdam, Netherlands)21(9)520-5(2016 Oct)
Trifa AP,B?nescu C,Dima D,Bojan AS,Tevet M,Moldovan VG,Vesa ?C,Murat M,Pop IV,Skrypnyk C,Popp RA

From Six Gene Polymorphisms of the Antioxidant System, Only GPX Pro198Leu and GSTP1 Ile105Val Modulate the Risk of Acute Myeloid Leukemia.
Oxidative medicine and cellular longevity20162536705(2016)
B?nescu C,Iancu M,Trifa AP,C?ndea M,Benedek Lazar E,Moldovan VG,Duicu C,Tripon F,Crauciuc A,Dobreanu M

Determination of nucleotide sequence of cDNA coding rat glutathione peroxidase and diminished expression of the mRNA in selenium deficient rat liver.
Biochemical and biophysical research communications154(3)1024-8(1988 Aug)
Yoshimura S,Takekoshi S,Watanabe K,Fujii-Kuriyama Y

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Gpx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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