gRNAs
The following Gls gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gls cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa39856 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001109968.1 | |
| Accession Version | NM_001109968.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | glutaminase kidney isoform, mitochondrial isoform b | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M65150.1, AC094675.11 and AY083459.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATGCGGC TGCGAGGCTC GGCGATGCTG CGGGAGCTGC TCTTGAGGCC GCCTGCCGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001103438.1 |
| CDS | 52..1863 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001109968.1 |
1 | ATGATGCGGC TGCGAGGCTC GGCGATGCTG CGGGAGCTGC TCTTGAGGCC GCCTGCCGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa40409 | |
| Clone ID Related Accession (Same CDS sequence) | NM_012569.2 | |
| Accession Version | NM_012569.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2025bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-26 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | glutaminase kidney isoform, mitochondrial isoform a | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M65150.1, AC094675.11, CB776615.1 and M22586.1. On Oct 16, 2007 this sequence version replaced NM_012569.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M65150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGATGCGGC TGCGAGGCTC GGCGATGCTG CGGGAGCTGC TCTTGAGGCC GCCTGCCGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_036701.2 |
| CDS | 52..2076 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_012569.2 |
1 | ATGATGCGGC TGCGAGGCTC GGCGATGCTG CGGGAGCTGC TCTTGAGGCC GCCTGCCGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa42906 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008767075.2 | |
| Accession Version | XM_008767075.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1692bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | glutaminase kidney isoform, mitochondrial isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767075.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGCGGC TGCGAGGCTC GGCGATGCTG CGGGAGCTGC TCTTGAGGCC GCCTGCCGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008765297.1 |
| CDS | 197..1888 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008767075.2 |
1 | ATGATGCGGC TGCGAGGCTC GGCGATGCTG CGGGAGCTGC TCTTGAGGCC GCCTGCCGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
MicroRNA-200c exacerbates the ischemia/reperfusion injury of heart through targeting the glutaminase (GLS)-mediated glutamine metabolism. |
A? Induces Excitotoxicity Mediated by APC/C-Cdh1 Depletion That Can Be Prevented by Glutaminase Inhibition Promoting Neuronal Survival. |
Expression of Gls and Gls2 glutaminase isoforms in astrocytes. |
STAT1 regulates human glutaminase 1 promoter activity through multiple binding sites in HIV-1 infected macrophages. |
