Gfi1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfi1
Entrez Gene ID	24388
Full Name	growth factor independent 1 transcriptional repressor
General protein information	Preferred Names growth factor independent 1 transcriptional repressor Names zinc finger protein Gfi-1 Growth factor independent-1 growth factor independent 1 transcription repressor growth factor independent protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	putative transcription factor that contains novel zinc-finger motif; may regulate the expression of genes active during mitosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008769918.2	XP_008768140.1	zinc finger protein Gfi-1 isoform X1
XM_008769920.2	XP_008768142.1	zinc finger protein Gfi-1 isoform X3
XM_017599074.1	XP_017454563.1	zinc finger protein Gfi-1 isoform X1
XM_008769917.2	XP_008768139.1	zinc finger protein Gfi-1 isoform X1
XM_008769919.1	XP_008768141.1	zinc finger protein Gfi-1 isoform X2
NM_012566.1	NP_036698.1	zinc finger protein Gfi-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GFI1	XP_513562.3
Gallus gallus (chicken)	GFI1	XP_426644.3
Xenopus tropicalis (tropical clawed frog)	gfi1	XP_002933849.1
Bos taurus (cattle)	GFI1	NP_001178241.1
Danio rerio (zebrafish)	gfi1ab	NP_958495.1
Homo sapiens (human)	GFI1	NP_005254.2
	GFI1	XP_001099597.1
Rattus norvegicus (Norway rat)	Gfi1	NP_036698.1
Canis lupus familiaris (dog)	GFI1	NP_001012737.1
Mus musculus (house mouse)	Gfi1	NP_034408.1

Gene Ontology
Bibliography

Related articles in PubMed

Low GFI1 expression in white blood cells of CP-CML patients at diagnosis is strongly associated with subsequent blastic transformation.
Kok CH, Watkins DB, Leclercq T, D'Andrea RJ, Hughes TP, White DL
Leukemia27(6)1427-30(2013 Jun)

The human GFI136N variant induces epigenetic changes at the Hoxa9 locus and accelerates K-RAS driven myeloproliferative disorder in mice.
Khandanpour C, Krongold J, Sch?tte J, Bouwman F, Vassen L, Gaudreau MC, Chen R, Calero-Nieto FJ, Diamanti E, Hannah R, Meyer SE, Grimes HL, van der Reijden BA, Jansen JH, Patel CV, Peeters JK, L?wenberg B, D?hrsen U, G?ttgens B, M?r?y T
Blood120(19)4006-17(2012 Nov)

A hypomorphic mutation in the Gfi1 transcriptional repressor results in a novel form of neutropenia.
Ordo?ez-Rueda D, J?nsson F, Mancardi DA, Zhao W, Malzac A, Liang Y, Bertosio E, Grenot P, Blanquet V, Sabrautzki S, de Angelis MH, M?resse S, Duprez E, Bruhns P, Malissen B, Malissen M
European journal of immunology42(9)2395-408(2012 Sep)

Growth factor independence 1 protects hematopoietic stem cells against apoptosis but also prevents the development of a myeloproliferative-like disease.
Khandanpour C, Kosan C, Gaudreau MC, D?hrsen U, H?bert J, Zeng H, M?r?y T
Stem cells (Dayton, Ohio)29(2)376-85(2011 Feb)

[Expression of growth-factor independence 1 in patients with leukemia and its significance].
Wang TT, Chen ZX, Cen JN, He J, Sheng HJ, Yao L
Zhongguo shi yan xue ye xue za zhi18(4)834-7(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gfi-1 is recruited to CDKN1A core promoter through Miz-1 and represses CDKN1A transcription.
Title: A role of Miz-1 in Gfi-1-mediated transcriptional repression of CDKN1A.

The structure clearly shows that Gfi-1 zinc fingers 3-5 bind into the major groove of the target DNA reminiscent of canonical C(2)H(2) zinc-finger domains.
Title: Solution structure of Gfi-1 zinc domain bound to consensus DNA.

role for auto- and trans-regulation of Gfi1 by GFI1 and GFI1B in maintaining the normal expression patterns of Gfi1
Title: Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells.

This protein targets a subset of genes differentiating hematopoietic lineages and plays a role in the hierarchy of factors governing stem cell differentiation.
Title: Targets of the transcriptional repressor oncoprotein Gfi-1.

The following Gfi1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfi1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00355	NM_012566.1 Latest version!	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
ORa42901	XM_008769917.2 Latest version!	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa42901	XM_008769918.2 Latest version!	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa42901	XM_017599074.1 Latest version!	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa42902	XM_008769919.1 Latest version!	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa42903	XM_008769920.2 Latest version!	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfi1 ( NM_012566.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036698.1 Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), mRNA.

Price & Availability↑

CloneID	ORa00355
Clone ID Related Accession (Same CDS sequence)	NM_012566.1
Accession Version	NM_012566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L06986.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L06986.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689605, SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TACGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036698.1
CDS	65..1336
Translation	MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPVPGRADGGAVSAGESKMEPRERLSPESQLTEAPDRASASPNSCEGSVCDPSSEFEDYWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGAESGRPAARYGSEQAAGGAGAGQPGSCGAASGATSAGGLGLYGDFAPAAAGLFERPSTAAGRLYQDRGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK

Target ORF information:

RefSeq Version	NM_012566.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012566.1

ORF Insert Sequence:

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TACGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1 ( XM_008769918.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768140.1 Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa42901
Clone ID Related Accession (Same CDS sequence)	XM_008769918.2 , XM_017599074.1 , XM_008769917.2
Accession Version	XM_008769918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768140.1
CDS	1431..2702
Translation	MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPVPGRADGGAVSAGESKMEPRERLSPESQLTEAPDRASASPNSCEGSVCDPSSEFEDYWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGAESGRPAARYGSEQAAGGAGAGQPGSCGAASGATSAGGLGLYGDFAPAAAGLFERPSTAAGRLYQDRGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK

Target ORF information:

RefSeq Version	XM_008769918.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769918.2

ORF Insert Sequence:

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1 ( XM_017599074.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454563.1 Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42901
Clone ID Related Accession (Same CDS sequence)	XM_008769918.2 , XM_017599074.1 , XM_008769917.2
Accession Version	XM_017599074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454563.1
CDS	1575..2846
Translation	MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPVPGRADGGAVSAGESKMEPRERLSPESQLTEAPDRASASPNSCEGSVCDPSSEFEDYWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGAESGRPAARYGSEQAAGGAGAGQPGSCGAASGATSAGGLGLYGDFAPAAAGLFERPSTAAGRLYQDRGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK

Target ORF information:

RefSeq Version	XM_017599074.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599074.1

ORF Insert Sequence:

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1 ( XM_008769917.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768139.1 Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X2, mRNA.

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CloneID	ORa42901
Clone ID Related Accession (Same CDS sequence)	XM_008769918.2 , XM_017599074.1 , XM_008769917.2
Accession Version	XM_008769917.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768139.1
CDS	306..1577
Translation	MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPVPGRADGGAVSAGESKMEPRERLSPESQLTEAPDRASASPNSCEGSVCDPSSEFEDYWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGAESGRPAARYGSEQAAGGAGAGQPGSCGAASGATSAGGLGLYGDFAPAAAGLFERPSTAAGRLYQDRGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGEKPHKCQVCGKAFSQSSNLITHSRKHTGFKPFGCDLCGKGFQRKVDLRRHRETQHGLK

Target ORF information:

RefSeq Version	XM_008769917.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769917.2

ORF Insert Sequence:

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTGAGAA GCCCCACAAA TGCCAGGTGT GTGGCAAAGC CTTCAGTCAG 
AGCTCCAACC TCATCACTCA TAGCAGAAAG CACACAGGTT TCAAGCCCTT TGGCTGTGAC 
CTGTGTGGGA AGGGCTTCCA GAGGAAGGTG GATCTCAGGA GGCACCGAGA GACTCAGCAT 
GGACTCAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1 ( XM_008769919.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768141.1 Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa42902
Clone ID Related Accession (Same CDS sequence)	XM_008769919.1
Accession Version	XM_008769919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGCTCACT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768141.1
CDS	1023..2126
Translation	MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPVPGRADGGAVSAGESKMEPRERLSPESQLTEAPDRASASPNSCEGSVCDPSSEFEDYWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGAESGRPAARYGSEQAAGGAGAGQPGSCGAASGATSAGGLGLYGDFAPAAAGLFERPSTAAGRLYQDRGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTGSL

Target ORF information:

RefSeq Version	XM_008769919.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769919.1

ORF Insert Sequence:

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGCTCACT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gfi1 ( XM_008769920.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768142.1 Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa42903
Clone ID Related Accession (Same CDS sequence)	XM_008769920.2
Accession Version	XM_008769920.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	zinc finger protein Gfi-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769920.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768142.1
CDS	1023..2120
Translation	MPRSFLVKSKKAHSYHQPRSPGPDYSLRLETVPVPGRADGGAVSAGESKMEPRERLSPESQLTEAPDRASASPNSCEGSVCDPSSEFEDYWRPPSPSVSPASEKSLCRSLDEAQPYTLPFKPYAWSGLAGSDLRHLVQSYRQCSALERSAGLSLFCERGAESGRPAARYGSEQAAGGAGAGQPGSCGAASGATSAGGLGLYGDFAPAAAGLFERPSTAAGRLYQDRGHELHADKSVGVKVESELLCTRLLLGGGSYKCIKCSKVFSTPHGLEVHVRRSHSGTRPFACEMCGKTFGHAVSLEQHKAVHSQERSFDCKICGKSFKRSSTLSTHLLIHSDTRPYPCQYCGKRFHQKSDMKKHTFIHTG

Target ORF information:

RefSeq Version	XM_008769920.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus growth factor independent 1 transcriptional repressor (Gfi1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769920.2

ORF Insert Sequence:

ATGCCGCGCT CATTCCTGGT CAAGAGCAAG AAGGCGCACA GCTACCACCA GCCGCGTTCT 
CCGGGGCCGG ACTACTCCCT GCGCCTGGAG ACCGTGCCTG TGCCGGGCAG AGCAGACGGC 
GGCGCTGTGA GTGCAGGAGA GTCGAAGATG GAGCCCCGAG AGCGTTTGTC CCCCGAGTCT 
CAGCTTACCG AGGCTCCAGA CAGAGCCTCC GCGTCCCCCA ACAGCTGCGA AGGCAGCGTT 
TGTGACCCGA GCTCCGAGTT CGAGGACTAC TGGAGGCCCC CTTCTCCCTC TGTATCTCCA 
GCGTCGGAGA AGTCGCTGTG TCGCTCTCTG GACGAAGCCC AGCCCTACAC GCTGCCTTTC 
AAGCCCTACG CGTGGAGCGG CCTCGCGGGG TCTGACCTGC GGCACCTGGT GCAGAGCTAC 
CGGCAGTGCA GCGCGCTGGA GCGCAGCGCT GGCCTGAGCC TCTTCTGCGA GCGCGGCGCG 
GAGTCCGGCC GCCCGGCCGC GCGCTACGGC TCGGAGCAGG CTGCGGGCGG AGCGGGTGCG 
GGACAGCCTG GGAGCTGCGG AGCAGCCAGC GGCGCCACCA GCGCTGGGGG CCTGGGGCTC 
TATGGCGACT TCGCGCCTGC GGCGGCCGGG CTGTTCGAGC GGCCGAGCAC AGCCGCAGGC 
CGGCTGTACC AAGATCGCGG CCACGAGCTG CACGCGGACA AGAGTGTGGG AGTCAAGGTG 
GAGTCCGAGC TGCTGTGCAC CCGCCTGCTG CTGGGCGGCG GCTCCTACAA ATGCATCAAG 
TGCAGCAAGG TGTTTTCCAC ACCGCATGGG CTGGAGGTGC ACGTGCGCCG GTCCCACAGC 
GGCACGAGAC CCTTTGCGTG CGAGATGTGC GGCAAGACTT TCGGGCACGC GGTGAGCCTG 
GAGCAACACA AGGCCGTACA CTCGCAGGAA CGCAGCTTTG ACTGTAAGAT CTGTGGCAAG 
AGCTTCAAGA GGTCATCCAC ACTGTCCACA CATCTACTCA TTCACTCGGA TACCCGGCCC 
TACCCCTGTC AGTACTGTGG CAAGAGGTTC CACCAGAAGT CAGACATGAA GAAACACACT 
TTCATCCACA CAGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Low GFI1 expression in white blood cells of CP-CML patients at diagnosis is strongly associated with subsequent blastic transformation.
Leukemia27(6)1427-30(2013 Jun)
Kok CH,Watkins DB,Leclercq T,D'Andrea RJ,Hughes TP,White DL

The human GFI136N variant induces epigenetic changes at the Hoxa9 locus and accelerates K-RAS driven myeloproliferative disorder in mice.
Blood120(19)4006-17(2012 Nov)
Khandanpour C,Krongold J,Sch?tte J,Bouwman F,Vassen L,Gaudreau MC,Chen R,Calero-Nieto FJ,Diamanti E,Hannah R,Meyer SE,Grimes HL,van der Reijden BA,Jansen JH,Patel CV,Peeters JK,L?wenberg B,D?hrsen U,G?ttgens B,M?r?y T

A hypomorphic mutation in the Gfi1 transcriptional repressor results in a novel form of neutropenia.
European journal of immunology42(9)2395-408(2012 Sep)
Ordo?ez-Rueda D,J?nsson F,Mancardi DA,Zhao W,Malzac A,Liang Y,Bertosio E,Grenot P,Blanquet V,Sabrautzki S,de Angelis MH,M?resse S,Duprez E,Bruhns P,Malissen B,Malissen M

Growth factor independence 1 protects hematopoietic stem cells against apoptosis but also prevents the development of a myeloproliferative-like disease.
Stem cells (Dayton, Ohio)29(2)376-85(2011 Feb)
Khandanpour C,Kosan C,Gaudreau MC,D?hrsen U,H?bert J,Zeng H,M?r?y T

[Expression of growth-factor independence 1 in patients with leukemia and its significance].
Zhongguo shi yan xue ye xue za zhi18(4)834-7(2010 Aug)
Wang TT,Chen ZX,Cen JN,He J,Sheng HJ,Yao L

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Gfi1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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