Gfap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gfap
Entrez Gene ID	24387
Full Name	glial fibrillary acidic protein
General protein information	Preferred Names glial fibrillary acidic protein Names glial fibrillary acidic protein glial fibrillary acidic protein alpha glial fibrillary acidic protein delta intermediate filament
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.1
Summary	intermediate filament protein specific for astrocytes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017009.2	NP_058705.2	glial fibrillary acidic protein

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GFAP	XP_848378.1
Mus musculus (house mouse)	Gfap	NP_034407.2
Rattus norvegicus (Norway rat)	Gfap	NP_058705.2
Danio rerio (zebrafish)	gfap	NP_571448.2
Pan troglodytes (chimpanzee)	GFAP	XP_511561.3
	GFAP	XP_001102095.2
Bos taurus (cattle)	GFAP	NP_776490.2
Gallus gallus (chicken)	GFAP	XP_418091.2
Homo sapiens (human)	GFAP	NP_002046.1

Gene Ontology
Bibliography

Related articles in PubMed

The cystine-glutamate exchanger (xCT, Slc7a11) is expressed in significant concentrations in a subpopulation of astrocytes in the mouse brain.
Ottestad-Hansen S, Hu QX, Follin-Arbelet VV, Bentea E, Sato H, Massie A, Zhou Y, Danbolt NC
Glia(2018 Jan)

Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats.
Sun L, Min L, Zhou H, Li M, Shao F, Wang W
Behavioural brain research333258-266(2017 Aug)

Adipose Tissue Graft Improves Early but not Late Stages of Nerve Regeneration.
Bloanc? V, Ceau?u AR, Jitariu AA, Barmayoun A, Mo? R, Cr?iniceanu Z, Bratu T
In vivo (Athens, Greece)31(4)649-655(2017 Jul-Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that GFAP exhibited enhanced stability upon the addition of two equivalents of each ligands with ceftriaxone imparting a more spontaneous interactions and a more ordered complex system than phenytoin
Title: Interactions of GFAP with ceftriaxone and phenytoin: SRCD and molecular docking and dynamic simulation.

Astrocyte expression of GLAST/GFAP was reduced via JAK1/JAK2/STAT3 signaling pathway after exposure to sevoflurane.
Title: Sevoflurane Inhibits Glutamate-Aspartate Transporter and Glial Fibrillary Acidic Protein Expression in Hippocampal Astrocytes of Neonatal Rats Through the Janus Kinase/Signal Transducer and Activator of Transcription (JAK/STAT) Pathway.

It is likely that a plastic change in GFAP expression in astrocytes selectively occurs around Oxytocin (OXT) neurons at proestrus and facilitates OXT release.
Title: Expression of glial fibrillary acidic protein in astrocytes of rat supraoptic nucleus throughout estrous cycle.

reduction of GFAP+ cell density was in agreement with a lower expression of GFAP protein
Title: Anorexia Reduces GFAP+ Cell Density in the Rat Hippocampus.

Upon ependymal stem cells differentiation, Cx50 expression favors glial cell fate, since higher expression levels, endogenous or by over-expression of Cx50, augmented the expression of the astrocyte marker GFAP and impaired the neuronal marker Tuj1.
Title: Connexin 50 Expression in Ependymal Stem Progenitor Cells after Spinal Cord Injury Activation.

The following Gfap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gfap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12647	NM_017009.2 Latest version!	Rattus norvegicus glial fibrillary acidic protein (Gfap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gfap ( NM_017009.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058705.2 Rattus norvegicus glial fibrillary acidic protein (Gfap), mRNA.

Price & Availability↑

CloneID	ORa12647
Clone ID Related Accession (Same CDS sequence)	NM_017009.2
Accession Version	NM_017009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	glial fibrillary acidic protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088851.1. On Oct 12, 2007 this sequence version replaced NM_017009.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC088851.1, U03700.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGGA GACGTATCAC CTCTGCACGC CGCTCCTATG CCTCCTCCGA GACGATGGTC 
AGGGGCCATG GTCCTACCAG ACACCTGGGT ACCATTCCGC GCCTCTCCCT GTCTCGAATG 
ACGCCTCCAC TCCCTGCCAG GGTAGACTTC TCCCTGGCCG GGGCGCTCAA TGCCGGCTTC 
AAAGAGACTC GGGCCAGCGA GCGTGCGGAG ATGATGGAGC TCAATGACCG CTTTGCTAGC 
TACATCGAGA AGGTCCGCTT CCTGGAACAG CAAAACAAGG CGCTGGCAGC TGAGCTGAAC 
CAGCTTCGAG CCAAGGAGCC CACCAAACTG GCTGACGTTT ACCAGGCAGA ACTTCGGGAG 
CTGCGGCTGC GTCTGGACCA GCTTACTACC AACAGTGCCC GGCTGGAGGT GGAGAGGGAC 
AATCTCACAC AGGACCTCGG CACCCTGAGG CAGAAGCTCC AAGATGAAAC CAACCTGAGG 
CTGGAGGCGG AGAACAACCT GGCTGTGTAC AGACAGGAGG CGGATGAAGC CACCTTGGCT 
CGTGTGGATC TGGAGAGGAA GGTTGAGTCG CTGGAGGAGG AGATCCAGTT CTTGAGGAAG 
ATCCATGAGG AGGAAGTTCG AGAACTCCAG GAGCAGCTGG CCCAGCAGCA GGTCCACGTG 
GAGATGGATG TGGCCAAGCC AGACCTCACA GCGGCTCTGA GAGAGATTCG CACTCAGTAC 
GAGGCAGTGG CCACCAGTAA CATGCAAGAA ACAGAAGAGT GGTATCGGTC CAAGTTTGCA 
GACCTCACAG ACGTTGCTTC CCGCAACGCA GAGCTGCTCC GCCAGGCCAA GCACGAGGCT 
AATGACTATC GCCGCCAACT GCAGGCCTTG ACCTGCGACC TTGAGTCCTT GCGCGGCACG 
AACGAGTCCT TGGAGAGGCA AATGCGCGAA CAGGAGGAGC GCCACGCGCG GGAGTCGGCG 
AGTTACCAGG AGGCACTCGC TCGGCTGGAG GAGGAGGGCC AAAGCCTCAA GGAGGAGATG 
GCCCGCCACC TGCAGGAGTA CCAGGATCTA CTCAACGTTA AGCTAGCCCT GGACATCGAG 
ATCGCCACCT ACAGGAAATT GCTGGAGGGC GAAGAAAACC GCATCACCAT TCCTGTACAG 
ACTTTCTCCA ACCTCCAGAT CCGAGAAACC AGCCTGGACA CCAAATCTGT GTCAGAAGGC 
CACCTCAAGA GGAACATCGT GGTAAAGACG GTGGAGATGC GGGATGGCGA GGTCATTAAG 
GAGTCGAAGC AGGAGCACAA GGATGTGATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058705.2
CDS	2..1294
Translation	MERRRITSARRSYASSETMVRGHGPTRHLGTIPRLSLSRMTPPLPARVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTTNSARLEVERDNLTQDLGTLRQKLQDETNLRLEAENNLAVYRQEADEATLARVDLERKVESLEEEIQFLRKIHEEEVRELQEQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDVASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM

Target ORF information:

RefSeq Version	NM_017009.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glial fibrillary acidic protein (Gfap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017009.2

ORF Insert Sequence:

ATGGAGCGGA GACGTATCAC CTCTGCACGC CGCTCCTATG CCTCCTCCGA GACGATGGTC 
AGGGGCCATG GTCCTACCAG ACACCTGGGT ACCATTCCGC GCCTCTCCCT GTCTCGAATG 
ACGCCTCCAC TCCCTGCCAG GGTAGACTTC TCCCTGGCCG GGGCGCTCAA TGCCGGCTTC 
AAAGAGACTC GGGCCAGCGA GCGTGCGGAG ATGATGGAGC TCAATGACCG CTTTGCTAGC 
TACATCGAGA AGGTCCGCTT CCTGGAACAG CAAAACAAGG CGCTGGCAGC TGAGCTGAAC 
CAGCTTCGAG CCAAGGAGCC CACCAAACTG GCTGACGTTT ACCAGGCAGA ACTTCGGGAG 
CTGCGGCTGC GTCTGGACCA GCTTACTACC AACAGTGCCC GGCTGGAGGT GGAGAGGGAC 
AATCTCACAC AGGACCTCGG CACCCTGAGG CAGAAGCTCC AAGATGAAAC CAACCTGAGG 
CTGGAGGCGG AGAACAACCT GGCTGTGTAC AGACAGGAGG CGGATGAAGC CACCTTGGCT 
CGTGTGGATC TGGAGAGGAA GGTTGAGTCG CTGGAGGAGG AGATCCAGTT CTTGAGGAAG 
ATCCATGAGG AGGAAGTTCG AGAACTCCAG GAGCAGCTGG CCCAGCAGCA GGTCCACGTG 
GAGATGGATG TGGCCAAGCC AGACCTCACA GCGGCTCTGA GAGAGATTCG CACTCAGTAC 
GAGGCAGTGG CCACCAGTAA CATGCAAGAA ACAGAAGAGT GGTATCGGTC CAAGTTTGCA 
GACCTCACAG ACGTTGCTTC CCGCAACGCA GAGCTGCTCC GCCAGGCCAA GCACGAGGCT 
AATGACTATC GCCGCCAACT GCAGGCCTTG ACCTGCGACC TTGAGTCCTT GCGCGGCACG 
AACGAGTCCT TGGAGAGGCA AATGCGCGAA CAGGAGGAGC GCCACGCGCG GGAGTCGGCG 
AGTTACCAGG AGGCACTCGC TCGGCTGGAG GAGGAGGGCC AAAGCCTCAA GGAGGAGATG 
GCCCGCCACC TGCAGGAGTA CCAGGATCTA CTCAACGTTA AGCTAGCCCT GGACATCGAG 
ATCGCCACCT ACAGGAAATT GCTGGAGGGC GAAGAAAACC GCATCACCAT TCCTGTACAG 
ACTTTCTCCA ACCTCCAGAT CCGAGAAACC AGCCTGGACA CCAAATCTGT GTCAGAAGGC 
CACCTCAAGA GGAACATCGT GGTAAAGACG GTGGAGATGC GGGATGGCGA GGTCATTAAG 
GAGTCGAAGC AGGAGCACAA GGATGTGATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The cystine-glutamate exchanger (xCT, Slc7a11) is expressed in significant concentrations in a subpopulation of astrocytes in the mouse brain.
Glia(2018 Jan)
Ottestad-Hansen S,Hu QX,Follin-Arbelet VV,Bentea E,Sato H,Massie A,Zhou Y,Danbolt NC

Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats.
Behavioural brain research333258-266(2017 Aug)
Sun L,Min L,Zhou H,Li M,Shao F,Wang W

Adipose Tissue Graft Improves Early but not Late Stages of Nerve Regeneration.
In vivo (Athens, Greece)31(4)649-655(2017 Jul-Aug)
Bloanc? V,Ceau?u AR,Jitariu AA,Barmayoun A,Mo? R,Cr?iniceanu Z,Bratu T

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Gfap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報