Gad1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gad1
Entrez Gene ID	24379
Full Name	glutamate decarboxylase 1
Synonyms	Gad67
General protein information	Preferred Names glutamate decarboxylase 1 Names glutamate decarboxylase 1 67 kDa glutamic acid decarboxylase GAD-67 Glutamate decarboxylase 1 (brain) glutamate decarboxylase 1 variant GAD67NT glutamate decarboxylase 67 kDa isoform glutamic acid decarboxylase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q22
Summary	plays a role in the biosynthesis of gamma-aminobutyric acid [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017007.1	NP_058703.1	glutamate decarboxylase 1
XM_017591454.1	XP_017446943.1	glutamate decarboxylase 1 isoform X1
XM_017591455.1	XP_017446944.1	glutamate decarboxylase 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GAD1	NP_000808.2
Rattus norvegicus (Norway rat)	Gad1	NP_058703.1
Canis lupus familiaris (dog)	GAD1	NP_001091012.1
Pan troglodytes (chimpanzee)	GAD1	NP_001029285.1
Xenopus tropicalis (tropical clawed frog)	gad1	XP_004917767.1
Gallus gallus (chicken)	GAD1	NP_990244.1
Drosophila melanogaster (fruit fly)	Gad1	NP_728931.1
Neurospora crassa	NCU06112	XP_959974.1
	GAD1	XP_001082995.1
Bos taurus (cattle)	GAD1	NP_001069224.1
Mus musculus (house mouse)	Gad1	NP_032103.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005866	XP_315894.2
Caenorhabditis elegans (roundworm)	unc-25	NP_499689.1
Magnaporthe oryzae (rice blast fungus)	MGG_03869	XP_003720021.1

Gene Ontology
Bibliography

Related articles in PubMed

High expression of hippocampal glutamic acid decarboxylase 67 mediates hypersensitivity of the hypothalamic-pituitary-adrenal axis in response to prenatal caffeine exposure in rats.
Xu D, Zhang C, He X, Guo Z, Hu D, Lu J, Cheng S, Wang H
Toxicology letters28339-51(2018 Feb)

Epigenetic Regulation of Glutamic Acid Decarboxylase 67 in a Hippocampal Circuit.
Subburaju S, Coleman AJ, Cunningham MG, Ruzicka WB, Benes FM
Cerebral cortex (New York, N.Y. : 1991)27(11)5284-5293(2017 Nov)

Injection of Toll-like receptor 4 siRNA into the ventrolateral periaqueductal gray attenuates withdrawal syndrome in morphine-dependent rats.
Liu QF, Li L, Guo YQ, Li X, Mou ZD, Wang X, Du GZ
Archives italiennes de biologie154(4)133-142(2016 Dec)

Acute high-intensity noise induces rapid Arc protein expression but fails to rapidly change GAD expression in amygdala and hippocampus of rats: Effects of treatment with D-cycloserine.
Kapolowicz MR, Thompson LT
Hearing research34269-79(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings indicate that modifying TLR4 gene expression in the periaqueductal gray stimulates expression of the downstream signaling molecule, GAD67, which decreases Glu levels and increases GABA levels. This mechanism may explain the inhibition of withdrawal syndrome in morphine-dependent rats.
Title: Injection of Toll-like receptor 4 siRNA into the ventrolateral periaqueductal gray attenuates withdrawal syndrome in morphine-dependent rats.

No changes in either Arc or GAD protein expression were observed in primary auditory cortex in the immediate post-noise exposure period, confirming other reports that auditory cortical plasticity may not occur until later in the development of tinnitus.
Title: Acute high-intensity noise induces rapid Arc protein expression but fails to rapidly change GAD expression in amygdala and hippocampus of rats: Effects of treatment with D-cycloserine.

Results from this study indicate that social subordination is associated with several region-specific alterations in GAD67 mRNA expression in central stress circuits.
Title: Chronic social subordination stress modulates glutamic acid decarboxylase (GAD) 67 mRNA expression in central stress circuits.

The GAD65-independent mechanism for targeting of GAD67 to synaptic vesicles in neurons is not functional in islet beta-cells.
Title: Compartmentalization of GABA synthesis by GAD67 differs between pancreatic beta cells and neurons.

study found that re-expression of GAD67 in neurons following stroke may play a role in aberrant cell cycle activation, consequently being pro-apoptotic
Title: Pro-apoptotic function of GABA-related transcripts following stroke.

The following Gad1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gad1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02473	NM_017007.1 Latest version!	Rattus norvegicus glutamate decarboxylase 1 (Gad1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$349.30 $499.00
ORa60257	XM_017591455.1 Latest version!	Rattus norvegicus glutamate decarboxylase 1 (Gad1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
ORa60257	XM_017591454.1 Latest version!	Rattus norvegicus glutamate decarboxylase 1 (Gad1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gad1 ( NM_017007.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058703.1 Rattus norvegicus glutamate decarboxylase 1 (Gad1), mRNA.

Price & Availability↑

CloneID	ORa02473
Clone ID Related Accession (Same CDS sequence)	NM_017007.1
Accession Version	NM_017007.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	glutamate decarboxylase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X57573.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X57573.1, X57572.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCTT CCACGCCTTC GCCTGCAACC TCCTCGAACG CGGGAGCGGA TCCTAATACT 
ACCAACCTGC GTCCTACAAC ATATGATACT TGGTGTGGCG TAGCCCATGG ATGCACCAGA 
AAACTGGGCC TGAAGATCTG TGGTTTCTTG CAAAGGACCA ATAGCCTGGA AGAGAAGAGT 
CGTCTTGTGA GCGCCTTCAG GGAGAGGCAG GCCTCCAAGA ACCTGCTTTC CTGTGAAAAC 
AGTGACCCTG GTGCCCGCTT CCGGCGCACA GAGACGGACT TCTCCAACCT GTTTGCTCAA 
GATCTGCTTC CAGCTAAGAA CGGGGAGGAG CAAACTGTGC AGTTCTTACT GGAGGTGGTT 
GACATACTCC TCAACTATGT CCGCAAGACG TTTGATCGCT CCACCAAGGT TTTGGACTTC 
CACCACCCAC ACCAGTTGCT GGAAGGCATG GAAGGTTTTA ATTTGGAGCT GTCTGACCAC 
CCCGAGTCTC TGGAGCAGAT CCTGGTTGAC TGTAGAGACA CCCTAAAGTA CGGGGTTCGC 
ACAGGTCACC CTCGGTTTTT CAACCAGCTC TCTACTGGTT TGGATATCAT TGGTTTAGCT 
GGCGAATGGC TGACATCAAC TGCCAATACC AATATGTTCA CATATGAAAT TGCACCCGTG 
TTTGTTCTTA TGGAACAGAT CACACTTAAG AAGATGCGAG AGATCATTGG ATGGTCAAAT 
AAAGATGGTG ATGGGATATT TTCTCCTGGG GGAGCCATAT CCAACATGTA CAGCATCATG 
GCGGCTCGGT ACAAGTACTT TCCAGAAGTG AAAACAAAAG GCATGGCGGC TGTGCCCAAA 
CTGGTCCTCT TCACCTCAGA ACACAGTCAC TATTCCATAA AGAAGGCTGG GGCTGCGCTT 
GGCTTTGGAA CTGACAATGT GATCCTGATA AAGTGCAATG AAAGGGGGAA GATAATTCCA 
GCTGATTTAG AGGCAAAAAT TCTTGATGCC AAACAAAAGG GATTTGTTCC CCTTTATGTC 
AATGCAACCG CAGGCACGAC TGTTTATGGA GCGTTTGATC CGATCCAGGA GATTGCAGAC 
ATATGTGAGA AATACAACCT TTGGCTGCAT GTGGATGCTG CCTGGGGTGG TGGGCTGCTC 
ATGTCCCGGA AGCATCGCCA CAAACTCAGC GGCATAGAAA GGGCCAATTC AGTCACCTGG 
AACCCTCACA AGATGATGGG TGTGCTGCTC CAGTGTTCTG CCATCCTGGT CAAGGAAAAG 
GGTATACTCC AAGGATGCAA CCAGATGTGT GCAGGCTACC TCTTCCAGCC AGACAAGCAG 
TATGACGTCT CCTACGATAC AGGGGACAAG GCGATTCAGT GTGGCCGCCA TGTGGACATC 
TTCAAGTTCT GGCTGATGTG GAAAGCAAAG GGCACTGTGG GATTTGAAAA TCAGATCAAC 
AAATGCCTGG AGCTGGCTGA ATACCTCTAT GCCAAGATTA AAAACAGAGA AGAGTTTGAG 
ATGGTTTTCA ATGGTGAGCC TGAGCACACA AATGTCTGTT TCTGGTACAT ACCACAAAGC 
CTCCGAGGGG TTCCAGACAG CCCTGAGCGA CGAGAAAAAC TACACAGGGT GGCTCCCAAG 
ATCAAAGCCC TGATGATGGA GTCAGGAACA ACCATGGTTG GTTACCAGCC TCAAGGGGAC 
AAGGCCAACT TCTTCCGGAT GGTCATCTCC AACCCAGCCG CCACCCAGTC TGACATCGAT 
TTCCTCATCG AGGAAATAGA GAGGTTGGGT CAAGATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058703.1
CDS	184..1965
Translation	MASSTPSPATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFRERQASKNLLSCENSDPGARFRRTETDFSNLFAQDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIIGWSNKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEHSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADLEAKILDAKQKGFVPLYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLSGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPERREKLHRVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	NM_017007.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate decarboxylase 1 (Gad1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017007.1

ORF Insert Sequence:

ATGGCATCTT CCACGCCTTC GCCTGCAACC TCCTCGAACG CGGGAGCGGA TCCTAATACT 
ACCAACCTGC GTCCTACAAC ATATGATACT TGGTGTGGCG TAGCCCATGG ATGCACCAGA 
AAACTGGGCC TGAAGATCTG TGGTTTCTTG CAAAGGACCA ATAGCCTGGA AGAGAAGAGT 
CGTCTTGTGA GCGCCTTCAG GGAGAGGCAG GCCTCCAAGA ACCTGCTTTC CTGTGAAAAC 
AGTGACCCTG GTGCCCGCTT CCGGCGCACA GAGACGGACT TCTCCAACCT GTTTGCTCAA 
GATCTGCTTC CAGCTAAGAA CGGGGAGGAG CAAACTGTGC AGTTCTTACT GGAGGTGGTT 
GACATACTCC TCAACTATGT CCGCAAGACG TTTGATCGCT CCACCAAGGT TTTGGACTTC 
CACCACCCAC ACCAGTTGCT GGAAGGCATG GAAGGTTTTA ATTTGGAGCT GTCTGACCAC 
CCCGAGTCTC TGGAGCAGAT CCTGGTTGAC TGTAGAGACA CCCTAAAGTA CGGGGTTCGC 
ACAGGTCACC CTCGGTTTTT CAACCAGCTC TCTACTGGTT TGGATATCAT TGGTTTAGCT 
GGCGAATGGC TGACATCAAC TGCCAATACC AATATGTTCA CATATGAAAT TGCACCCGTG 
TTTGTTCTTA TGGAACAGAT CACACTTAAG AAGATGCGAG AGATCATTGG ATGGTCAAAT 
AAAGATGGTG ATGGGATATT TTCTCCTGGG GGAGCCATAT CCAACATGTA CAGCATCATG 
GCGGCTCGGT ACAAGTACTT TCCAGAAGTG AAAACAAAAG GCATGGCGGC TGTGCCCAAA 
CTGGTCCTCT TCACCTCAGA ACACAGTCAC TATTCCATAA AGAAGGCTGG GGCTGCGCTT 
GGCTTTGGAA CTGACAATGT GATCCTGATA AAGTGCAATG AAAGGGGGAA GATAATTCCA 
GCTGATTTAG AGGCAAAAAT TCTTGATGCC AAACAAAAGG GATTTGTTCC CCTTTATGTC 
AATGCAACCG CAGGCACGAC TGTTTATGGA GCGTTTGATC CGATCCAGGA GATTGCAGAC 
ATATGTGAGA AATACAACCT TTGGCTGCAT GTGGATGCTG CCTGGGGTGG TGGGCTGCTC 
ATGTCCCGGA AGCATCGCCA CAAACTCAGC GGCATAGAAA GGGCCAATTC AGTCACCTGG 
AACCCTCACA AGATGATGGG TGTGCTGCTC CAGTGTTCTG CCATCCTGGT CAAGGAAAAG 
GGTATACTCC AAGGATGCAA CCAGATGTGT GCAGGCTACC TCTTCCAGCC AGACAAGCAG 
TATGACGTCT CCTACGATAC AGGGGACAAG GCGATTCAGT GTGGCCGCCA TGTGGACATC 
TTCAAGTTCT GGCTGATGTG GAAAGCAAAG GGCACTGTGG GATTTGAAAA TCAGATCAAC 
AAATGCCTGG AGCTGGCTGA ATACCTCTAT GCCAAGATTA AAAACAGAGA AGAGTTTGAG 
ATGGTTTTCA ATGGTGAGCC TGAGCACACA AATGTCTGTT TCTGGTACAT ACCACAAAGC 
CTCCGAGGGG TTCCAGACAG CCCTGAGCGA CGAGAAAAAC TACACAGGGT GGCTCCCAAG 
ATCAAAGCCC TGATGATGGA GTCAGGAACA ACCATGGTTG GTTACCAGCC TCAAGGGGAC 
AAGGCCAACT TCTTCCGGAT GGTCATCTCC AACCCAGCCG CCACCCAGTC TGACATCGAT 
TTCCTCATCG AGGAAATAGA GAGGTTGGGT CAAGATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gad1 ( XM_017591454.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446943.1 Rattus norvegicus glutamate decarboxylase 1 (Gad1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60257
Clone ID Related Accession (Same CDS sequence)	XM_017591454.1 , XM_017591455.1
Accession Version	XM_017591454.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate decarboxylase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTT CCACGCCTTC GCCTGCAACC TCCTCGAACG CGGGAGCGGA TCCTAATACT 
ACCAACCTGC GTCCTACAAC ATATGATACT TGGTGTGGCG TAGCCCATGG ATGCACCAGA 
AAACTGGGCC TGAAGATCTG TGGCTTCTTG CAAAGGACCA ATAGCCTGGA AGAGAAGAGT 
CGTCTTGTGA GTGCCTTCAG GGAGAGGCAG GCCTCCAAGA ACCTGCTTTC CTGTGAAAAC 
AGTGACCCTG GTGCCCGCTT CCGGCGCACA GAGACGGACT TCTCCAACCT GTTTGCTCAA 
GATCTGCTTC CAGCTAAGAA CGGGGAGGAG CAAACTGTGC AGTTCTTACT GGAGGTGGTT 
GACATACTCC TCAACTATGT CCGCAAGACG TTTGATCGCT CCACCAAGGT TTTGGACTTC 
CACCACCCAC ACCAGTTGCT GGAAGGCATG GAAGGTTTTA ATTTGGAGCT GTCTGACCAC 
CCCGAGTCTC TGGAGCAGAT CCTGGTTGAC TGTAGAGACA CCCTAAAGTA CGGGGTTCGC 
ACAGGTCACC CTCGGTTTTT CAACCAGCTC TCTACTGGTT TGGATATCAT TGGTTTAGCT 
GGCGAATGGC TGACATCAAC TGCCAATACC AATATGTTCA CATATGAAAT TGCACCCGTG 
TTTGTTCTTA TGGAACAGAT TACACTTAAG AAGATGCGAG AGATCATTGG ATGGTCAAAT 
AAAGATGGTG ATGGGATATT TTCTCCTGGG GGAGCCATAT CCAACATGTA CAGCATCATG 
GCGGCTCGGT ACAAGTACTT TCCAGAAGTG AAAACAAAAG GCATGGCGGC TGTGCCCAAA 
CTGGTCCTCT TCACCTCAGA ACACAGTCAC TATTCCATAA AGAAGGCTGG GGCTGCGCTT 
GGCTTTGGAA CTGACAATGT GATCCTGATA AAGTGCAATG AAAGGGGGAA GATAATTCCA 
GCTGATTTAG AGGCAAAAAT TCTTGATGCC AAACAAAAGG GATTTGTTCC CCTTTATGTC 
AATGCAACCG CAGGCACGAC TGTTTATGGA GCGTTTGATC CGATCCAGGA GATTGCAGAC 
ATATGTGAGA AATACAACCT TTGGCTGCAT GTGGATGCTG CCTGGGGTGG TGGGCTGCTC 
ATGTCCCGGA AGCATCGCCA CAAACTCAGC GGCATAGAAA GGGCCAATTC AGTCACCTGG 
AACCCTCACA AGATGATGGG TGTGCTGCTC CAGTGTTCTG CCATCCTGGT CAAGGAAAAG 
GGTATACTCC AAGGATGCAA CCAGATGTGT GCAGGCTACC TCTTCCAGCC AGACAAGCAG 
TATGACGTCT CCTACGATAC AGGGGACAAG GCGATTCAGT GTGGCCGCCA TGTGGACATC 
TTCAAGTTCT GGCTGATGTG GAAAGCAAAG GGCACTGTGG GATTTGAAAA TCAGATCAAC 
AAATGCCTGG AGCTGGCTGA ATACCTCTAT GCCAAGATTA AAAACAGAGA AGAGTTTGAG 
ATGGTTTTCA ATGGTGAGCC TGAGCACACA AATGTCTGTT TCTGGTACAT ACCACAAAGC 
CTCCGAGGGG TTCCAGACAG CCCTGAGCGA CGAGAAAAAC TACACAGGGT GGCTCCCAAG 
ATCAAAGCCC TGATGATGGA GTCAGGAACA ACCATGGTTG GTTACCAGCC TCAAGGGGAC 
AAGGCCAACT TCTTCCGGAT GGTCATCTCC AACCCAGCCG CCACCCAGTC TGACATCGAT 
TTCCTCATCG AGGAAATAGA GAGGTTGGGT CAAGATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446943.1
CDS	286..2067
Translation	MASSTPSPATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFRERQASKNLLSCENSDPGARFRRTETDFSNLFAQDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIIGWSNKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEHSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADLEAKILDAKQKGFVPLYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLSGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPERREKLHRVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	XM_017591454.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate decarboxylase 1 (Gad1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591454.1

ORF Insert Sequence:

ATGGCATCTT CCACGCCTTC GCCTGCAACC TCCTCGAACG CGGGAGCGGA TCCTAATACT 
ACCAACCTGC GTCCTACAAC ATATGATACT TGGTGTGGCG TAGCCCATGG ATGCACCAGA 
AAACTGGGCC TGAAGATCTG TGGCTTCTTG CAAAGGACCA ATAGCCTGGA AGAGAAGAGT 
CGTCTTGTGA GTGCCTTCAG GGAGAGGCAG GCCTCCAAGA ACCTGCTTTC CTGTGAAAAC 
AGTGACCCTG GTGCCCGCTT CCGGCGCACA GAGACGGACT TCTCCAACCT GTTTGCTCAA 
GATCTGCTTC CAGCTAAGAA CGGGGAGGAG CAAACTGTGC AGTTCTTACT GGAGGTGGTT 
GACATACTCC TCAACTATGT CCGCAAGACG TTTGATCGCT CCACCAAGGT TTTGGACTTC 
CACCACCCAC ACCAGTTGCT GGAAGGCATG GAAGGTTTTA ATTTGGAGCT GTCTGACCAC 
CCCGAGTCTC TGGAGCAGAT CCTGGTTGAC TGTAGAGACA CCCTAAAGTA CGGGGTTCGC 
ACAGGTCACC CTCGGTTTTT CAACCAGCTC TCTACTGGTT TGGATATCAT TGGTTTAGCT 
GGCGAATGGC TGACATCAAC TGCCAATACC AATATGTTCA CATATGAAAT TGCACCCGTG 
TTTGTTCTTA TGGAACAGAT TACACTTAAG AAGATGCGAG AGATCATTGG ATGGTCAAAT 
AAAGATGGTG ATGGGATATT TTCTCCTGGG GGAGCCATAT CCAACATGTA CAGCATCATG 
GCGGCTCGGT ACAAGTACTT TCCAGAAGTG AAAACAAAAG GCATGGCGGC TGTGCCCAAA 
CTGGTCCTCT TCACCTCAGA ACACAGTCAC TATTCCATAA AGAAGGCTGG GGCTGCGCTT 
GGCTTTGGAA CTGACAATGT GATCCTGATA AAGTGCAATG AAAGGGGGAA GATAATTCCA 
GCTGATTTAG AGGCAAAAAT TCTTGATGCC AAACAAAAGG GATTTGTTCC CCTTTATGTC 
AATGCAACCG CAGGCACGAC TGTTTATGGA GCGTTTGATC CGATCCAGGA GATTGCAGAC 
ATATGTGAGA AATACAACCT TTGGCTGCAT GTGGATGCTG CCTGGGGTGG TGGGCTGCTC 
ATGTCCCGGA AGCATCGCCA CAAACTCAGC GGCATAGAAA GGGCCAATTC AGTCACCTGG 
AACCCTCACA AGATGATGGG TGTGCTGCTC CAGTGTTCTG CCATCCTGGT CAAGGAAAAG 
GGTATACTCC AAGGATGCAA CCAGATGTGT GCAGGCTACC TCTTCCAGCC AGACAAGCAG 
TATGACGTCT CCTACGATAC AGGGGACAAG GCGATTCAGT GTGGCCGCCA TGTGGACATC 
TTCAAGTTCT GGCTGATGTG GAAAGCAAAG GGCACTGTGG GATTTGAAAA TCAGATCAAC 
AAATGCCTGG AGCTGGCTGA ATACCTCTAT GCCAAGATTA AAAACAGAGA AGAGTTTGAG 
ATGGTTTTCA ATGGTGAGCC TGAGCACACA AATGTCTGTT TCTGGTACAT ACCACAAAGC 
CTCCGAGGGG TTCCAGACAG CCCTGAGCGA CGAGAAAAAC TACACAGGGT GGCTCCCAAG 
ATCAAAGCCC TGATGATGGA GTCAGGAACA ACCATGGTTG GTTACCAGCC TCAAGGGGAC 
AAGGCCAACT TCTTCCGGAT GGTCATCTCC AACCCAGCCG CCACCCAGTC TGACATCGAT 
TTCCTCATCG AGGAAATAGA GAGGTTGGGT CAAGATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gad1 ( XM_017591455.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446944.1 Rattus norvegicus glutamate decarboxylase 1 (Gad1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60257
Clone ID Related Accession (Same CDS sequence)	XM_017591454.1 , XM_017591455.1
Accession Version	XM_017591455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glutamate decarboxylase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCTT CCACGCCTTC GCCTGCAACC TCCTCGAACG CGGGAGCGGA TCCTAATACT 
ACCAACCTGC GTCCTACAAC ATATGATACT TGGTGTGGCG TAGCCCATGG ATGCACCAGA 
AAACTGGGCC TGAAGATCTG TGGCTTCTTG CAAAGGACCA ATAGCCTGGA AGAGAAGAGT 
CGTCTTGTGA GTGCCTTCAG GGAGAGGCAG GCCTCCAAGA ACCTGCTTTC CTGTGAAAAC 
AGTGACCCTG GTGCCCGCTT CCGGCGCACA GAGACGGACT TCTCCAACCT GTTTGCTCAA 
GATCTGCTTC CAGCTAAGAA CGGGGAGGAG CAAACTGTGC AGTTCTTACT GGAGGTGGTT 
GACATACTCC TCAACTATGT CCGCAAGACG TTTGATCGCT CCACCAAGGT TTTGGACTTC 
CACCACCCAC ACCAGTTGCT GGAAGGCATG GAAGGTTTTA ATTTGGAGCT GTCTGACCAC 
CCCGAGTCTC TGGAGCAGAT CCTGGTTGAC TGTAGAGACA CCCTAAAGTA CGGGGTTCGC 
ACAGGTCACC CTCGGTTTTT CAACCAGCTC TCTACTGGTT TGGATATCAT TGGTTTAGCT 
GGCGAATGGC TGACATCAAC TGCCAATACC AATATGTTCA CATATGAAAT TGCACCCGTG 
TTTGTTCTTA TGGAACAGAT TACACTTAAG AAGATGCGAG AGATCATTGG ATGGTCAAAT 
AAAGATGGTG ATGGGATATT TTCTCCTGGG GGAGCCATAT CCAACATGTA CAGCATCATG 
GCGGCTCGGT ACAAGTACTT TCCAGAAGTG AAAACAAAAG GCATGGCGGC TGTGCCCAAA 
CTGGTCCTCT TCACCTCAGA ACACAGTCAC TATTCCATAA AGAAGGCTGG GGCTGCGCTT 
GGCTTTGGAA CTGACAATGT GATCCTGATA AAGTGCAATG AAAGGGGGAA GATAATTCCA 
GCTGATTTAG AGGCAAAAAT TCTTGATGCC AAACAAAAGG GATTTGTTCC CCTTTATGTC 
AATGCAACCG CAGGCACGAC TGTTTATGGA GCGTTTGATC CGATCCAGGA GATTGCAGAC 
ATATGTGAGA AATACAACCT TTGGCTGCAT GTGGATGCTG CCTGGGGTGG TGGGCTGCTC 
ATGTCCCGGA AGCATCGCCA CAAACTCAGC GGCATAGAAA GGGCCAATTC AGTCACCTGG 
AACCCTCACA AGATGATGGG TGTGCTGCTC CAGTGTTCTG CCATCCTGGT CAAGGAAAAG 
GGTATACTCC AAGGATGCAA CCAGATGTGT GCAGGCTACC TCTTCCAGCC AGACAAGCAG 
TATGACGTCT CCTACGATAC AGGGGACAAG GCGATTCAGT GTGGCCGCCA TGTGGACATC 
TTCAAGTTCT GGCTGATGTG GAAAGCAAAG GGCACTGTGG GATTTGAAAA TCAGATCAAC 
AAATGCCTGG AGCTGGCTGA ATACCTCTAT GCCAAGATTA AAAACAGAGA AGAGTTTGAG 
ATGGTTTTCA ATGGTGAGCC TGAGCACACA AATGTCTGTT TCTGGTACAT ACCACAAAGC 
CTCCGAGGGG TTCCAGACAG CCCTGAGCGA CGAGAAAAAC TACACAGGGT GGCTCCCAAG 
ATCAAAGCCC TGATGATGGA GTCAGGAACA ACCATGGTTG GTTACCAGCC TCAAGGGGAC 
AAGGCCAACT TCTTCCGGAT GGTCATCTCC AACCCAGCCG CCACCCAGTC TGACATCGAT 
TTCCTCATCG AGGAAATAGA GAGGTTGGGT CAAGATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446944.1
CDS	90..1871
Translation	MASSTPSPATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFRERQASKNLLSCENSDPGARFRRTETDFSNLFAQDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIIGWSNKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEHSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADLEAKILDAKQKGFVPLYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLSGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPERREKLHRVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	XM_017591455.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate decarboxylase 1 (Gad1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591455.1

ORF Insert Sequence:

ATGGCATCTT CCACGCCTTC GCCTGCAACC TCCTCGAACG CGGGAGCGGA TCCTAATACT 
ACCAACCTGC GTCCTACAAC ATATGATACT TGGTGTGGCG TAGCCCATGG ATGCACCAGA 
AAACTGGGCC TGAAGATCTG TGGCTTCTTG CAAAGGACCA ATAGCCTGGA AGAGAAGAGT 
CGTCTTGTGA GTGCCTTCAG GGAGAGGCAG GCCTCCAAGA ACCTGCTTTC CTGTGAAAAC 
AGTGACCCTG GTGCCCGCTT CCGGCGCACA GAGACGGACT TCTCCAACCT GTTTGCTCAA 
GATCTGCTTC CAGCTAAGAA CGGGGAGGAG CAAACTGTGC AGTTCTTACT GGAGGTGGTT 
GACATACTCC TCAACTATGT CCGCAAGACG TTTGATCGCT CCACCAAGGT TTTGGACTTC 
CACCACCCAC ACCAGTTGCT GGAAGGCATG GAAGGTTTTA ATTTGGAGCT GTCTGACCAC 
CCCGAGTCTC TGGAGCAGAT CCTGGTTGAC TGTAGAGACA CCCTAAAGTA CGGGGTTCGC 
ACAGGTCACC CTCGGTTTTT CAACCAGCTC TCTACTGGTT TGGATATCAT TGGTTTAGCT 
GGCGAATGGC TGACATCAAC TGCCAATACC AATATGTTCA CATATGAAAT TGCACCCGTG 
TTTGTTCTTA TGGAACAGAT TACACTTAAG AAGATGCGAG AGATCATTGG ATGGTCAAAT 
AAAGATGGTG ATGGGATATT TTCTCCTGGG GGAGCCATAT CCAACATGTA CAGCATCATG 
GCGGCTCGGT ACAAGTACTT TCCAGAAGTG AAAACAAAAG GCATGGCGGC TGTGCCCAAA 
CTGGTCCTCT TCACCTCAGA ACACAGTCAC TATTCCATAA AGAAGGCTGG GGCTGCGCTT 
GGCTTTGGAA CTGACAATGT GATCCTGATA AAGTGCAATG AAAGGGGGAA GATAATTCCA 
GCTGATTTAG AGGCAAAAAT TCTTGATGCC AAACAAAAGG GATTTGTTCC CCTTTATGTC 
AATGCAACCG CAGGCACGAC TGTTTATGGA GCGTTTGATC CGATCCAGGA GATTGCAGAC 
ATATGTGAGA AATACAACCT TTGGCTGCAT GTGGATGCTG CCTGGGGTGG TGGGCTGCTC 
ATGTCCCGGA AGCATCGCCA CAAACTCAGC GGCATAGAAA GGGCCAATTC AGTCACCTGG 
AACCCTCACA AGATGATGGG TGTGCTGCTC CAGTGTTCTG CCATCCTGGT CAAGGAAAAG 
GGTATACTCC AAGGATGCAA CCAGATGTGT GCAGGCTACC TCTTCCAGCC AGACAAGCAG 
TATGACGTCT CCTACGATAC AGGGGACAAG GCGATTCAGT GTGGCCGCCA TGTGGACATC 
TTCAAGTTCT GGCTGATGTG GAAAGCAAAG GGCACTGTGG GATTTGAAAA TCAGATCAAC 
AAATGCCTGG AGCTGGCTGA ATACCTCTAT GCCAAGATTA AAAACAGAGA AGAGTTTGAG 
ATGGTTTTCA ATGGTGAGCC TGAGCACACA AATGTCTGTT TCTGGTACAT ACCACAAAGC 
CTCCGAGGGG TTCCAGACAG CCCTGAGCGA CGAGAAAAAC TACACAGGGT GGCTCCCAAG 
ATCAAAGCCC TGATGATGGA GTCAGGAACA ACCATGGTTG GTTACCAGCC TCAAGGGGAC 
AAGGCCAACT TCTTCCGGAT GGTCATCTCC AACCCAGCCG CCACCCAGTC TGACATCGAT 
TTCCTCATCG AGGAAATAGA GAGGTTGGGT CAAGATCTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High expression of hippocampal glutamic acid decarboxylase 67 mediates hypersensitivity of the hypothalamic-pituitary-adrenal axis in response to prenatal caffeine exposure in rats.
Toxicology letters28339-51(2018 Feb)
Xu D,Zhang C,He X,Guo Z,Hu D,Lu J,Cheng S,Wang H

Epigenetic Regulation of Glutamic Acid Decarboxylase 67 in a Hippocampal Circuit.
Cerebral cortex (New York, N.Y. : 1991)27(11)5284-5293(2017 Nov)
Subburaju S,Coleman AJ,Cunningham MG,Ruzicka WB,Benes FM

Injection of Toll-like receptor 4 siRNA into the ventrolateral periaqueductal gray attenuates withdrawal syndrome in morphine-dependent rats.
Archives italiennes de biologie154(4)133-142(2016 Dec)
Liu QF,Li L,Guo YQ,Li X,Mou ZD,Wang X,Du GZ

Acute high-intensity noise induces rapid Arc protein expression but fails to rapidly change GAD expression in amygdala and hippocampus of rats: Effects of treatment with D-cycloserine.
Hearing research34269-79(2016 Dec)
Kapolowicz MR,Thompson LT