Egfr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egfr
Entrez Gene ID	24329
Full Name	epidermal growth factor receptor
Synonyms	ERBB1,ErbB-1,Errp
General protein information	Preferred Names epidermal growth factor receptor Names epidermal growth factor receptor EGFR-related peptide Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1) avian erythroblastic leukemia viral (v-erbB) oncogene homolog epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22
Summary	promotes cell proliferation and differentiation; mediates GPCR regulated induction of protein synthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599073.1	XP_017454562.1	epidermal growth factor receptor isoform X2
XM_008770416.2	XP_008768638.1	epidermal growth factor receptor isoform X1
XM_008770418.2	XP_008768640.1	epidermal growth factor receptor isoform X3
NM_031507.1	NP_113695.1	epidermal growth factor receptor precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	EGFR	XP_533073.3
Pan troglodytes (chimpanzee)	EGFR	XP_519102.3
Bos taurus (cattle)	EGFR	XP_002696936.2
Mus musculus (house mouse)	Egfr	NP_997538.1
Gallus gallus (chicken)	EGFR	NP_990828.2
Danio rerio (zebrafish)	egfra	NP_919405.1
	EGFR	XP_001107305.1
Rattus norvegicus (Norway rat)	Egfr	NP_113695.1
Homo sapiens (human)	EGFR	NP_005219.2
Xenopus tropicalis (tropical clawed frog)	egfr	XP_002939960.2

Gene Ontology
Bibliography

Related articles in PubMed

Src-mediated ligand release-independent EGFR transactivation involves TGF-?-induced Smad3 activation in mesangial cells.
Chen Y, Peng FF, Jin J, Chen HM, Yu H, Zhang BF
Biochemical and biophysical research communications493(2)914-920(2017 11)

Noise Stress Induces an Epidermal Growth Factor Receptor/Xeroderma Pigmentosum-A Response in the Auditory Nerve.
Guthrie OW
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society65(3)173-184(2017 Mar)

Radiolabeled novel mAb 4G1 for immunoSPECT imaging of EGFRvIII expression in preclinical glioblastoma xenografts.
Liu X, Dong C, Shi J, Ma T, Jin Z, Jia B, Liu Z, Shen L, Wang F
Oncotarget8(4)6364-6375(2017 Jan)

Epidermal Growth Factor Receptor Silencing Blunts the Slow Force Response to Myocardial Stretch.
Brea MS, D?az RG, Escudero DS, Caldiz CI, Portiansky EL, Morgan PE, P?rez NG
Journal of the American Heart Association5(10)(2016 Oct)

Periodic Mechanical Stress Activates PKC?-Dependent EGFR Mitogenic Signals in Rat Chondrocytes via PI3K-Akt and ERK1/2.
He P, Shen N, Gao G, Jiang X, Sun H, Zhou D, Xu N, Nong L, Ren K
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(4)1281-94(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This paper provides evidence that TGF-beta stimulates prolonged EGFR transactivation, which is dependent on c-Src tyrosine kinase (Src).
Title: Src-mediated ligand release-independent EGFR transactivation involves TGF-?-induced Smad3 activation in mesangial cells.

This current study tested the hypothesis that noise stress would alter the expression pattern of EGFR/XPA within the auditory nerve. In both the apical and middle cochlear coils, the noise stress depleted EGFR+XPA expression. The noise stress caused a significant increase in the proportion of neurons that expressed EGFR-alone in the middle coils.
Title: Noise Stress Induces an Epidermal Growth Factor Receptor/Xeroderma Pigmentosum-A Response in the Auditory Nerve.

Results provide evidence that specific EGFR activation after myocardial stretch is a key factor in promoting the redox-sensitive kinase activation pathway, leading to slow force response development.
Title: Epidermal Growth Factor Receptor Silencing Blunts the Slow Force Response to Myocardial Stretch.

These results highlight the essential role of NF-kappaB-dependent transcriptional upregulation of cyclin D1 in EGFR-mediated cytoprotective effects under hypoxic preconditioning.
Title: NF-?B-dependent transcriptional upregulation of cyclin D1 exerts cytoprotection against hypoxic injury upon EGFR activation.

The findings suggested that periodic mechanical stress promoted chondrocyte proliferation through PKCdelta-EGFR-PI3K-Akt-ERK1/2.
Title: Periodic Mechanical Stress Activates PKC?-Dependent EGFR Mitogenic Signals in Rat Chondrocytes via PI3K-Akt and ERK1/2.

The following Egfr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egfr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42896	XM_008770418.2 Latest version!	Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa58223	XM_017599073.1 Latest version!	Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa13100	NM_031507.1 Latest version!	Rattus norvegicus epidermal growth factor receptor (Egfr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$888.30 $1269.00
ORa42894	XM_008770416.2 Latest version!	Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egfr ( XM_008770418.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768640.1 Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa42896
Clone ID Related Accession (Same CDS sequence)	XM_008770418.2
Accession Version	XM_008770418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	epidermal growth factor receptor isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCAGT TATGCAACCG TTGCTGTTGT GTCCATCCAT GGAGAATAAT TAACAGAGCA 
GCCATTTCCG ATTTCACTGA TCTCGACCTG GATGGTTTTA CGGGACATCC TGTGTTGCTT 
TGTTATATTG GACGTGCAGG CAGAGAGCAC GCTGGGTCTT CTCGGAATCC ATTCTCCCAC 
TCAGGAGTTT GCCAAGGCAC AAGTAACAGG CTCACCCAAC TAGGCACCTT TGAAGACCAC 
TTTCTGAGCC TCCAGAGGAT GTTCAACAAC TGTGAAGTGG TCCTTGGAAA CTTGGAAATC 
ACCTATGTGC AAAGGAATTA TGACCTTTCC TTCTTAAAGA CCATTCAGGA GGTGGCTGGC 
TATGTTCTCA TTGCCCTGAA CACCGTGGAG AGAATCCCTT TGGAGAACCT GCAGATCATC 
AGGGGAAATG CTCTCTACGA AAACACCTAC GCCTTAGCCG TCCTGTCCAA CTATGGAACC 
AACAAAACTG GGCTTAGGGA ACTGCCCATG CGGAACTTAC AGGAAATTCT GATCGGTGCT 
GTGCGATTTA GCAACAACCC CATCCTCTGC AATATGGAGA CCATCCAGTG GAGGGACATC 
GTCCAAGATG TCTTTCTGAG CAACATGTCA ATGGACGTAC AGCGCCACCT GACCGGCTGC 
CCGAAATGTG ATCCGAGCTG TCCCAATGGA AGCTGCTGGG GAAGAGGAGA GGAGAACTGC 
CAGAAATTGA CCAAAATCAT CTGCGCCCAG CAATGTTCCC GGCGTTGTCG TGGCAGGTCC 
CCTAGCGACT GCTGCCACAA CCAGTGTGCC GCAGGGTGTA CAGGGCCCAG AGAGAGTGAC 
TGTCTGGTCT GCCACAGGTT CCGAGATGAA GCCACGTGCA AAGACACCTG CCCACCACTC 
ATGCTGTACA ACCCCACCAC GTACCAGATG GATGTCAACC CTGAGGGGAA GTACAGCTTT 
GGTGCCACCT GTGTGAAGAA ATGCCCCAGA AACTACGTGG TGACAGATCA CGGCTCGTGT 
GTCCGGGCCT GTGGGCCAGA CTACTATGAA GTAGAAGAAG ATGGAGTCAG CAAGTGTAAA 
AAATGTGATG GGCCCTGCCG CAAAGTTTGC AATGGCATAG GCATTGGTGA ATTTAAAGAC 
ACACTCTCCA TAAATGCTAC AAACATCAAA CACTTCAAGT ACTGCACTGC CATCAGTGGG 
GACCTCCACA TCCTGCCAGT GGCCTTTAAG GGGGATTCTT TCACCCGCAC TCCTCCTCTA 
GACCCACGGG AACTAGAAAT TCTCAAAACT GTGAAGGAAA TAACAGGGTT TTTGCTGATT 
CAGGCTTGGC CTGAAAACTG GACTGACCTC CATGCTTTTG AGAACCTAGA AATAATTCGT 
GGCAGAACAA AGCAACATGG TCAGTTTTCT CTGGCGGTTG TCGGCCTGAA CATAACATCG 
CTGGGGTTGC GTTCCCTCAA GGAGATCAGT GATGGGGATG TGATTATTTC TGGGAACCGA 
AATTTGTGCT ACGCAAACAC TATAAACTGG AAAAAACTCT TCGGGACGCC CAATCAAAAG 
ACCAAAATCA TGAACAACAG AGCTGAAAAG GACTGCAAGG CCACGAACCA CGTCTGTAAT 
CCTTTATGCT CCTCGGAAGG CTGCTGGGGC CCTGAGCCCA CGGACTGTGT CTCCTGCCAG 
AATGTGAGCA GAGGCAGGGA GTGCGTGGAC AAGTGCAACA TCCTGGAGGG GGAACCGAGG 
GAGTTTGTGG AAAATTCTGA ATGCATCCAG TGCCATCCAG AATGTCTGCC CCAGACCATG 
AACATCACCT GTACAGGCCG GGGGCCAGAC AACTGCATCA AGTGTGCCCA CTATGTTGAT 
GGTCCCCACT GTGTCAAGAC CTGCCCTTCG GGCATCATGG GGGAGAACAA CACCCTGGTC 
TGGAAGTTTG CAGATGCCAA TAACGTCTGC CACCTCTGCC ATGCAAACTG TACCTATGGA 
TGTGCTGGGC CAGGCCTTAA AGGATGTCAA CAACCAGAAG GTTCCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768640.1
CDS	8910..10943
Translation	MFQLCNRCCCVHPWRIINRAAISDFTDLDLDGFTGHPVLLCYIGRAGREHAGSSRNPFSHSGVCQGTSNRLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAVLSNYGTNKTGLRELPMRNLQEILIGAVRFSNNPILCNMETIQWRDIVQDVFLSNMSMDVQRHLTGCPKCDPSCPNGSCWGRGEENCQKLTKIICAQQCSRRCRGRSPSDCCHNQCAAGCTGPRESDCLVCHRFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGVSKCKKCDGPCRKVCNGIGIGEFKDTLSINATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPENWTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKIMNNRAEKDCKATNHVCNPLCSSEGCWGPEPTDCVSCQNVSRGRECVDKCNILEGEPREFVENSECIQCHPECLPQTMNITCTGRGPDNCIKCAHYVDGPHCVKTCPSGIMGENNTLVWKFADANNVCHLCHANCTYGCAGPGLKGCQQPEGSNY

Target ORF information:

RefSeq Version	XM_008770418.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770418.2

ORF Insert Sequence:

ATGTTCCAGT TATGCAACCG TTGCTGTTGT GTCCATCCAT GGAGAATAAT TAACAGAGCA 
GCCATTTCCG ATTTCACTGA TCTCGACCTG GATGGTTTTA CGGGACATCC TGTGTTGCTT 
TGTTATATTG GACGTGCAGG CAGAGAGCAC GCTGGGTCTT CTCGGAATCC ATTCTCCCAC 
TCAGGAGTTT GCCAAGGCAC AAGTAACAGG CTCACCCAAC TAGGCACCTT TGAAGACCAC 
TTTCTGAGCC TCCAGAGGAT GTTCAACAAC TGTGAAGTGG TCCTTGGAAA CTTGGAAATC 
ACCTATGTGC AAAGGAATTA TGACCTTTCC TTCTTAAAGA CCATTCAGGA GGTGGCTGGC 
TATGTTCTCA TTGCCCTGAA CACCGTGGAG AGAATCCCTT TGGAGAACCT GCAGATCATC 
AGGGGAAATG CTCTCTACGA AAACACCTAC GCCTTAGCCG TCCTGTCCAA CTATGGAACC 
AACAAAACTG GGCTTAGGGA ACTGCCCATG CGGAACTTAC AGGAAATTCT GATCGGTGCT 
GTGCGATTTA GCAACAACCC CATCCTCTGC AATATGGAGA CCATCCAGTG GAGGGACATC 
GTCCAAGATG TCTTTCTGAG CAACATGTCA ATGGACGTAC AGCGCCACCT GACCGGCTGC 
CCGAAATGTG ATCCGAGCTG TCCCAATGGA AGCTGCTGGG GAAGAGGAGA GGAGAACTGC 
CAGAAATTGA CCAAAATCAT CTGCGCCCAG CAATGTTCCC GGCGTTGTCG TGGCAGGTCC 
CCTAGCGACT GCTGCCACAA CCAGTGTGCC GCAGGGTGTA CAGGGCCCAG AGAGAGTGAC 
TGTCTGGTCT GCCACAGGTT CCGAGATGAA GCCACGTGCA AAGACACCTG CCCACCACTC 
ATGCTGTACA ACCCCACCAC GTACCAGATG GATGTCAACC CTGAGGGGAA GTACAGCTTT 
GGTGCCACCT GTGTGAAGAA ATGCCCCAGA AACTACGTGG TGACAGATCA CGGCTCGTGT 
GTCCGGGCCT GTGGGCCAGA CTACTATGAA GTAGAAGAAG ATGGAGTCAG CAAGTGTAAA 
AAATGTGATG GGCCCTGCCG CAAAGTTTGC AATGGCATAG GCATTGGTGA ATTTAAAGAC 
ACACTCTCCA TAAATGCTAC AAACATCAAA CACTTCAAGT ACTGCACTGC CATCAGTGGG 
GACCTCCACA TCCTGCCAGT GGCCTTTAAG GGGGATTCTT TCACCCGCAC TCCTCCTCTA 
GACCCACGGG AACTAGAAAT TCTCAAAACT GTGAAGGAAA TAACAGGGTT TTTGCTGATT 
CAGGCTTGGC CTGAAAACTG GACTGACCTC CATGCTTTTG AGAACCTAGA AATAATTCGT 
GGCAGAACAA AGCAACATGG TCAGTTTTCT CTGGCGGTTG TCGGCCTGAA CATAACATCG 
CTGGGGTTGC GTTCCCTCAA GGAGATCAGT GATGGGGATG TGATTATTTC TGGGAACCGA 
AATTTGTGCT ACGCAAACAC TATAAACTGG AAAAAACTCT TCGGGACGCC CAATCAAAAG 
ACCAAAATCA TGAACAACAG AGCTGAAAAG GACTGCAAGG CCACGAACCA CGTCTGTAAT 
CCTTTATGCT CCTCGGAAGG CTGCTGGGGC CCTGAGCCCA CGGACTGTGT CTCCTGCCAG 
AATGTGAGCA GAGGCAGGGA GTGCGTGGAC AAGTGCAACA TCCTGGAGGG GGAACCGAGG 
GAGTTTGTGG AAAATTCTGA ATGCATCCAG TGCCATCCAG AATGTCTGCC CCAGACCATG 
AACATCACCT GTACAGGCCG GGGGCCAGAC AACTGCATCA AGTGTGCCCA CTATGTTGAT 
GGTCCCCACT GTGTCAAGAC CTGCCCTTCG GGCATCATGG GGGAGAACAA CACCCTGGTC 
TGGAAGTTTG CAGATGCCAA TAACGTCTGC CACCTCTGCC ATGCAAACTG TACCTATGGA 
TGTGCTGGGC CAGGCCTTAA AGGATGTCAA CAACCAGAAG GTTCCAACTA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfr ( XM_017599073.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454562.1 Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa58223
Clone ID Related Accession (Same CDS sequence)	XM_017599073.1
Accession Version	XM_017599073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	epidermal growth factor receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGCGG CAGCACAGTG GTTCGCCCTT CCCTCTTCTC CCTCGCACCT CTGTGCCTGG 
ATTTCCTTGC CCTGCTGTCT GTCCCTGGGC TGCTGCCTGG CAGTTTGCCA AGGCACAAGT 
AACAGGCTCA CCCAACTAGG CACCTTTGAA GACCACTTTC TGAGCCTCCA GAGGATGTTC 
AACAACTGTG AAGTGGTCCT TGGAAACTTG GAAATCACCT ATGTGCAAAG GAATTATGAC 
CTTTCCTTCT TAAAGACCAT TCAGGAGGTG GCTGGCTATG TTCTCATTGC CCTGAACACC 
GTGGAGAGAA TCCCTTTGGA GAACCTGCAG ATCATCAGGG GAAATGCTCT CTACGAAAAC 
ACCTACGCCT TAGCCGTCCT GTCCAACTAT GGAACCAACA AAACTGGGCT TAGGGAACTG 
CCCATGCGGA ACTTACAGGA AATTCTGATC GGTGCTGTGC GATTTAGCAA CAACCCCATC 
CTCTGCAATA TGGAGACCAT CCAGTGGAGG GACATCGTCC AAGATGTCTT TCTGAGCAAC 
ATGTCAATGG ACGTACAGCG CCACCTGACC GGCTGCCCGA AATGTGATCC GAGCTGTCCC 
AATGGAAGCT GCTGGGGAAG AGGAGAGGAG AACTGCCAGA AATTGACCAA AATCATCTGC 
GCCCAGCAAT GTTCCCGGCG TTGTCGTGGC AGGTCCCCTA GCGACTGCTG CCACAACCAG 
TGTGCCGCAG GGTGTACAGG GCCCAGAGAG AGTGACTGTC TGGTCTGCCA CAGGTTCCGA 
GATGAAGCCA CGTGCAAAGA CACCTGCCCA CCACTCATGC TGTACAACCC CACCACGTAC 
CAGATGGATG TCAACCCTGA GGGGAAGTAC AGCTTTGGTG CCACCTGTGT GAAGAAATGC 
CCCAGAAACT ACGTGGTGAC AGATCACGGC TCGTGTGTCC GGGCCTGTGG GCCAGACTAC 
TATGAAGTAG AAGAAGATGG AGTCAGCAAG TGTAAAAAAT GTGATGGGCC CTGCCGCAAA 
GTTTGCAATG GCATAGGCAT TGGTGAATTT AAAGACACAC TCTCCATAAA TGCTACAAAC 
ATCAAACACT TCAAGTACTG CACTGCCATC AGTGGGGACC TCCACATCCT GCCAGTGGCC 
TTTAAGGGGG ATTCTTTCAC CCGCACTCCT CCTCTAGACC CACGGGAACT AGAAATTCTC 
AAAACTGTGA AGGAAATAAC AGGGTTTTTG CTGATTCAGG CTTGGCCTGA AAACTGGACT 
GACCTCCATG CTTTTGAGAA CCTAGAAATA ATTCGTGGCA GAACAAAGCA ACATGGTCAG 
TTTTCTCTGG CGGTTGTCGG CCTGAACATA ACATCGCTGG GGTTGCGTTC CCTCAAGGAG 
ATCAGTGATG GGGATGTGAT TATTTCTGGG AACCGAAATT TGTGCTACGC AAACACTATA 
AACTGGAAAA AACTCTTCGG GACGCCCAAT CAAAAGACCA AAATCATGAA CAACAGAGCT 
GAAAAGGACT GCAAGGCCAC GAACCACGTC TGTAATCCTT TATGCTCCTC GGAAGGCTGC 
TGGGGCCCTG AGCCCACGGA CTGTGTCTCC TGCCAGAATG TGAGCAGAGG CAGGGAGTGC 
GTGGACAAGT GCAACATCCT GGAGGGGGAA CCGAGGGAGT TTGTGGAAAA TTCTGAATGC 
ATCCAGTGCC ATCCAGAATG TCTGCCCCAG ACCATGAACA TCACCTGTAC AGGCCGGGGG 
CCAGACAACT GCATCAAGTG TGCCCACTAT GTTGATGGTC CCCACTGTGT CAAGACCTGC 
CCTTCGGGCA TCATGGGGGA GAACAACACC CTGGTCTGGA AGTTTGCAGA TGCCAATAAC 
GTCTGCCACC TCTGCCATGC AAACTGTACC TATGGATGTG CTGGGCCAGG CCTTAAAGGA 
TGTCAACAAC CAGAAGGGCC AAAGATCCCA TCCATCGCCA CTGGGATTGT GGGTGGCCTC 
CTCTTCATAG TAGTGGTGGC CCTTGGGATC GGCCTCTTCA TGCGAAGGCG TCACATTGTC 
CGAAAACGTA CACTACGCCG CCTGCTTCAA GAGAGAGAGC TCGTGGAACC TCTCACACCC 
AGCGGAGAAG CTCCGAACCA AGCCCACTTG AGGATATTAA AGGAAACAGA ATTCAAAAAG 
ATCAAAGTTC TGGGTTCAGG AGCATTTGGC ACAGTGTATA AGGGTCTCTG GATCCCAGAA 
GGCGAGAAAG TGAAAATCCC TGTGGCCATC AAGGAGTTAA GAGAAGCCAC ATCTCCCAAA 
GCCAACAAGG AAATCCTTGA TGAAGCCTAC GTGATGGCCA GTGTGGACAA CCCTCATGTA 
TGCCGCCTCC TGGGCATCTG TCTGACCTCC ACTGTCCAGC TCATTACACA ACTCATGCCC 
TATGGTTGCC TCCTGGACTA TGTCCGAGAA CATAAGGACA ACATTGGCTC CCAGTACCTA 
CTCAACTGGT GTGTGCAGAT TGCAAAGGGC ATGAACTACC TGGAAGACCG GCGTTTGGTA 
CACCGTGACT TGGCAGCCAG GAATGTACTG GTAAAGACAC CACAGCATGT CAAGATCACA 
GATTTTGGAC TGGCCAAACT GCTTGGTGCT GAGGAGAAAG AATACCATGC AGAGGGGGGC 
AAAGTGCCTA TCAAGTGGAT GGCTTTGGAA TCAATTTTAC ACCGAATTTA TACACACCAA 
AGCGACGTCT GGAGCTATGG AGTCACCGTG TGGGAACTGA TGACCTTTGG GTCCAAGCCT 
TATGATGGGA TCCCTGCAAG TGAGATCTCA TCCATCCTAG AGAAAGGAGA GCGCCTTCCA 
CAGCCACCTA TCTGCACCAT CGACGTCTAC ATGATCATGG TCAAGTGCTG GATGATAGAT 
GCTGATAGCC GCCCAAAGTT CCGAGAGTTG ATTCTCGAAT TCTCCAAAAT GGCCAGAGAC 
CCACAGCGCT ACCTTGTTAT CCAGGGGGAT GAAAGGATGC ATTTGCCGAG CCCTACAGAC 
TCCAACTTTT ACCGAGCCCT GATGGAGGAG GAGGACATGG AAGACGTAGT TGATGCTGAT 
GAATACCTCA TCCCACAGCA AGGCTTCTTC AACAGCCCAT CCACGTCACG GACTCCACTC 
TTGAGCTCTC TGAGTGCAAA TAGCAACAGT TCCGCTGTGG CTTGCATTAA TAGAAATGGG 
AGCTGCCGTG TCAAAGAAGA CGCCTTCTTG CAACGGTATA GCTCCGATCC CACCAGCGTC 
CTGACAGAGG ACAACATAGA TGACACATTC CTTCCCGTGC CTGAATATAT AAACCAATCT 
GTTCCCAAGA GGCCGGCTGG CTCTGTGCAG AACCCAGTCT ATCACAATCA GCCCCTGCAT 
CCAGCTCCTG GAAGAGACCT GCATTATCAA AATCCCCATA GCAATGCGGT GAGCAACCCT 
GAGTATCTCA ACACTGCCCA GCCGACCTGC CTCAGTAGTG GGTTTGACAG CTCTGCCCTC 
TGGATCCAGA AAGGCAGCCA CCAAATGAGC CTGGACAACC CTGACTACCA GCAGGACTTC 
TTTCCCAAAG AAGCCAAGCC GAATGGCATC TTTAAGGGCC CCACAGCTGA AAATGCAGAG 
TACCTGCGGG TGGCACCGCC AAGCAGTGAG TTTATTGGAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454562.1
CDS	138..3782
Translation	MGAAAQWFALPSSPSHLCAWISLPCCLSLGCCLAVCQGTSNRLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAVLSNYGTNKTGLRELPMRNLQEILIGAVRFSNNPILCNMETIQWRDIVQDVFLSNMSMDVQRHLTGCPKCDPSCPNGSCWGRGEENCQKLTKIICAQQCSRRCRGRSPSDCCHNQCAAGCTGPRESDCLVCHRFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGVSKCKKCDGPCRKVCNGIGIGEFKDTLSINATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPENWTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKIMNNRAEKDCKATNHVCNPLCSSEGCWGPEPTDCVSCQNVSRGRECVDKCNILEGEPREFVENSECIQCHPECLPQTMNITCTGRGPDNCIKCAHYVDGPHCVKTCPSGIMGENNTLVWKFADANNVCHLCHANCTYGCAGPGLKGCQQPEGPKIPSIATGIVGGLLFIVVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELILEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMEEEDMEDVVDADEYLIPQQGFFNSPSTSRTPLLSSLSANSNSSAVACINRNGSCRVKEDAFLQRYSSDPTSVLTEDNIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLHPAPGRDLHYQNPHSNAVSNPEYLNTAQPTCLSSGFDSSALWIQKGSHQMSLDNPDYQQDFFPKEAKPNGIFKGPTAENAEYLRVAPPSSEFIGA

Target ORF information:

RefSeq Version	XM_017599073.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599073.1

ORF Insert Sequence:

ATGGGAGCGG CAGCACAGTG GTTCGCCCTT CCCTCTTCTC CCTCGCACCT CTGTGCCTGG 
ATTTCCTTGC CCTGCTGTCT GTCCCTGGGC TGCTGCCTGG CAGTTTGCCA AGGCACAAGT 
AACAGGCTCA CCCAACTAGG CACCTTTGAA GACCACTTTC TGAGCCTCCA GAGGATGTTC 
AACAACTGTG AAGTGGTCCT TGGAAACTTG GAAATCACCT ATGTGCAAAG GAATTATGAC 
CTTTCCTTCT TAAAGACCAT TCAGGAGGTG GCTGGCTATG TTCTCATTGC CCTGAACACC 
GTGGAGAGAA TCCCTTTGGA GAACCTGCAG ATCATCAGGG GAAATGCTCT CTACGAAAAC 
ACCTACGCCT TAGCCGTCCT GTCCAACTAT GGAACCAACA AAACTGGGCT TAGGGAACTG 
CCCATGCGGA ACTTACAGGA AATTCTGATC GGTGCTGTGC GATTTAGCAA CAACCCCATC 
CTCTGCAATA TGGAGACCAT CCAGTGGAGG GACATCGTCC AAGATGTCTT TCTGAGCAAC 
ATGTCAATGG ACGTACAGCG CCACCTGACC GGCTGCCCGA AATGTGATCC GAGCTGTCCC 
AATGGAAGCT GCTGGGGAAG AGGAGAGGAG AACTGCCAGA AATTGACCAA AATCATCTGC 
GCCCAGCAAT GTTCCCGGCG TTGTCGTGGC AGGTCCCCTA GCGACTGCTG CCACAACCAG 
TGTGCCGCAG GGTGTACAGG GCCCAGAGAG AGTGACTGTC TGGTCTGCCA CAGGTTCCGA 
GATGAAGCCA CGTGCAAAGA CACCTGCCCA CCACTCATGC TGTACAACCC CACCACGTAC 
CAGATGGATG TCAACCCTGA GGGGAAGTAC AGCTTTGGTG CCACCTGTGT GAAGAAATGC 
CCCAGAAACT ACGTGGTGAC AGATCACGGC TCGTGTGTCC GGGCCTGTGG GCCAGACTAC 
TATGAAGTAG AAGAAGATGG AGTCAGCAAG TGTAAAAAAT GTGATGGGCC CTGCCGCAAA 
GTTTGCAATG GCATAGGCAT TGGTGAATTT AAAGACACAC TCTCCATAAA TGCTACAAAC 
ATCAAACACT TCAAGTACTG CACTGCCATC AGTGGGGACC TCCACATCCT GCCAGTGGCC 
TTTAAGGGGG ATTCTTTCAC CCGCACTCCT CCTCTAGACC CACGGGAACT AGAAATTCTC 
AAAACTGTGA AGGAAATAAC AGGGTTTTTG CTGATTCAGG CTTGGCCTGA AAACTGGACT 
GACCTCCATG CTTTTGAGAA CCTAGAAATA ATTCGTGGCA GAACAAAGCA ACATGGTCAG 
TTTTCTCTGG CGGTTGTCGG CCTGAACATA ACATCGCTGG GGTTGCGTTC CCTCAAGGAG 
ATCAGTGATG GGGATGTGAT TATTTCTGGG AACCGAAATT TGTGCTACGC AAACACTATA 
AACTGGAAAA AACTCTTCGG GACGCCCAAT CAAAAGACCA AAATCATGAA CAACAGAGCT 
GAAAAGGACT GCAAGGCCAC GAACCACGTC TGTAATCCTT TATGCTCCTC GGAAGGCTGC 
TGGGGCCCTG AGCCCACGGA CTGTGTCTCC TGCCAGAATG TGAGCAGAGG CAGGGAGTGC 
GTGGACAAGT GCAACATCCT GGAGGGGGAA CCGAGGGAGT TTGTGGAAAA TTCTGAATGC 
ATCCAGTGCC ATCCAGAATG TCTGCCCCAG ACCATGAACA TCACCTGTAC AGGCCGGGGG 
CCAGACAACT GCATCAAGTG TGCCCACTAT GTTGATGGTC CCCACTGTGT CAAGACCTGC 
CCTTCGGGCA TCATGGGGGA GAACAACACC CTGGTCTGGA AGTTTGCAGA TGCCAATAAC 
GTCTGCCACC TCTGCCATGC AAACTGTACC TATGGATGTG CTGGGCCAGG CCTTAAAGGA 
TGTCAACAAC CAGAAGGGCC AAAGATCCCA TCCATCGCCA CTGGGATTGT GGGTGGCCTC 
CTCTTCATAG TAGTGGTGGC CCTTGGGATC GGCCTCTTCA TGCGAAGGCG TCACATTGTC 
CGAAAACGTA CACTACGCCG CCTGCTTCAA GAGAGAGAGC TCGTGGAACC TCTCACACCC 
AGCGGAGAAG CTCCGAACCA AGCCCACTTG AGGATATTAA AGGAAACAGA ATTCAAAAAG 
ATCAAAGTTC TGGGTTCAGG AGCATTTGGC ACAGTGTATA AGGGTCTCTG GATCCCAGAA 
GGCGAGAAAG TGAAAATCCC TGTGGCCATC AAGGAGTTAA GAGAAGCCAC ATCTCCCAAA 
GCCAACAAGG AAATCCTTGA TGAAGCCTAC GTGATGGCCA GTGTGGACAA CCCTCATGTA 
TGCCGCCTCC TGGGCATCTG TCTGACCTCC ACTGTCCAGC TCATTACACA ACTCATGCCC 
TATGGTTGCC TCCTGGACTA TGTCCGAGAA CATAAGGACA ACATTGGCTC CCAGTACCTA 
CTCAACTGGT GTGTGCAGAT TGCAAAGGGC ATGAACTACC TGGAAGACCG GCGTTTGGTA 
CACCGTGACT TGGCAGCCAG GAATGTACTG GTAAAGACAC CACAGCATGT CAAGATCACA 
GATTTTGGAC TGGCCAAACT GCTTGGTGCT GAGGAGAAAG AATACCATGC AGAGGGGGGC 
AAAGTGCCTA TCAAGTGGAT GGCTTTGGAA TCAATTTTAC ACCGAATTTA TACACACCAA 
AGCGACGTCT GGAGCTATGG AGTCACCGTG TGGGAACTGA TGACCTTTGG GTCCAAGCCT 
TATGATGGGA TCCCTGCAAG TGAGATCTCA TCCATCCTAG AGAAAGGAGA GCGCCTTCCA 
CAGCCACCTA TCTGCACCAT CGACGTCTAC ATGATCATGG TCAAGTGCTG GATGATAGAT 
GCTGATAGCC GCCCAAAGTT CCGAGAGTTG ATTCTCGAAT TCTCCAAAAT GGCCAGAGAC 
CCACAGCGCT ACCTTGTTAT CCAGGGGGAT GAAAGGATGC ATTTGCCGAG CCCTACAGAC 
TCCAACTTTT ACCGAGCCCT GATGGAGGAG GAGGACATGG AAGACGTAGT TGATGCTGAT 
GAATACCTCA TCCCACAGCA AGGCTTCTTC AACAGCCCAT CCACGTCACG GACTCCACTC 
TTGAGCTCTC TGAGTGCAAA TAGCAACAGT TCCGCTGTGG CTTGCATTAA TAGAAATGGG 
AGCTGCCGTG TCAAAGAAGA CGCCTTCTTG CAACGGTATA GCTCCGATCC CACCAGCGTC 
CTGACAGAGG ACAACATAGA TGACACATTC CTTCCCGTGC CTGAATATAT AAACCAATCT 
GTTCCCAAGA GGCCGGCTGG CTCTGTGCAG AACCCAGTCT ATCACAATCA GCCCCTGCAT 
CCAGCTCCTG GAAGAGACCT GCATTATCAA AATCCCCATA GCAATGCGGT GAGCAACCCT 
GAGTATCTCA ACACTGCCCA GCCGACCTGC CTCAGTAGTG GGTTTGACAG CTCTGCCCTC 
TGGATCCAGA AAGGCAGCCA CCAAATGAGC CTGGACAACC CTGACTACCA GCAGGACTTC 
TTTCCCAAAG AAGCCAAGCC GAATGGCATC TTTAAGGGCC CCACAGCTGA AAATGCAGAG 
TACCTGCGGG TGGCACCGCC AAGCAGTGAG TTTATTGGAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfr ( NM_031507.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113695.1 Rattus norvegicus epidermal growth factor receptor (Egfr), mRNA.

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CloneID	ORa13100
Clone ID Related Accession (Same CDS sequence)	NM_031507.1
Accession Version	NM_031507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	epidermal growth factor receptor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M37394.2. On Jan 12, 2005 this sequence version replaced XM_346468.1. Sequence Note: This sequence has been modified as follows: removed 33 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM801042.1, HM801041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGACCCT CAGGGACTGC GAGAACCAAG CTACTGCTGC TGCTGGCTGC GCTCTGCGCC 
GCAGGTGGGG CGCTGGAGGA AAAGAAAGTT TGCCAAGGCA CAAGTAACAG GCTCACCCAA 
CTAGGCACCT TTGAAGACCA CTTTCTGAGC CTCCAGAGGA TGTTCAACAA CTGTGAAGTG 
GTCCTTGGAA ACTTGGAAAT CACCTATGTG CAAAGGAATT ATGACCTTTC CTTCTTAAAG 
ACCATCCAGG AGGTGGCTGG CTATGTTCTC ATTGCCCTGA ACACCGTGGA GAGAATCCCT 
TTGGAGAACC TGCAGATCAT CAGGGGAAAT GCTCTCTACG AAAACACCTA CGCCTTAGCC 
GTCCTGTCCA ACTATGGAAC CAACAAAACT GGGCTTAGGG AACTGCCCAT GCGGAACTTA 
CAGGAAATTC TGATCGGTGC TGTGCGATTT AGCAACAACC CCATCCTCTG CAATATGGAG 
ACCATCCAGT GGAGGGACAT CGTCCAAGAT GTCTTTCTGA GCAACATGTC AATGGACGTA 
CAGCGCCACC TGACGGGCTG CCCGAAATGT GATCCGAGCT GTCCCAATGG AAGCTGCTGG 
GGAAGAGGAG AGGAGAACTG CCAGAAATTG ACCAAAATCA TCTGCGCCCA GCAATGTTCC 
CGGCGTTGTC GTGGCAGGTC CCCTAGCGAC TGCTGCCACA ACCAGTGTGC CGCAGGGTGT 
ACAGGGCCCA GAGAGAGTGA CTGTCTGGTC TGCCACAGGT TCCGAGATGA AGCCACGTGC 
AAAGACACCT GCCCACCACT CATGCTGTAC AACCCCACCA CGTACCAGAT GGATGTCAAC 
CCTGAGGGGA AGTACAGCTT TGGTGCCACC TGTGTGAAGA AATGCCCCAG AAACTACGTG 
GTGACAGATC ACGGCTCGTG TGTCCGGGCC TGTGGGCCAG ACTACTATGA AGTAGAAGAA 
GATGGAGTCA GCAAGTGTAA AAAATGTGAC GGGCCCTGCC GCAAAGTTTG CAATGGCATA 
GGCATTGGTG AATTTAAAGA CACACTCTCC ATAAATGCTA CAAACATCAA ACACTTCAAG 
TACTGCACTG CCATCAGTGG GGACCTCCAC ATCCTGCCAG TGGCCTTTAA GGGGGATTCT 
TTCACCCGCA CTCCTCCTCT AGACCCACGG GAACTAGAAA TTCTCAAAAC TGTGAAGGAA 
ATAACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACT GGACTGACCT CCATGCTTTT 
GAGAACCTAG AAATAATTCG TGGCAGAACA AAGCAACATG GTCAGTTTTC TCTGGCGGTT 
GTCGGCCTGA ACATAACATC GCTGGGGTTG CGTTCCCTCA AGGAGATCAG TGATGGGGAT 
GTGATTATTT CTGGGAACCG AAATTTGTGC TACGCAAACA CTATAAACTG GAAAAAACTC 
TTCGGGACGC CCAATCAAAA GACCAAAATC ATGAACAACA GAGCTGAAAA GGACTGCAAG 
GCCACGAACC ACGTCTGTAA TCCTTTATGC TCCTCGGAAG GCTGCTGGGG CCCTGAGCCC 
ACGGACTGTG TCTCCTGCCA GAATGTGAGC AGAGGCAGGG AGTGCGTGGA CAAGTGCAAC 
ATCCTGGAGG GGGAACCGAG GGAGTTTGTG GAAAATTCTG AATGCATCCA GTGCCATCCA 
GAATGTCTGC CCCAGACCAT GAACATCACC TGTACAGGCC GGGGGCCAGA CAACTGCATC 
AAGTGTGCCC ACTATGTTGA TGGTCCCCAC TGTGTCAAGA CCTGCCCTTC GGGCATCATG 
GGGGAGAACA ACACCCTGGT CTGGAAGTTT GCAGATGCCA ATAACGTCTG CCACCTCTGC 
CATGCAAACT GTACCTATGG ATGTGCTGGG CCAGGCCTTA AAGGATGTCA ACAACCAGAA 
GGGCCAAAGA TCCCATCCAT CGCCACTGGG ATTGTGGGTG GCCTCCTCTT CATAGTAGTG 
GTGGCCCTTG GGATCGGCCT CTTCATGCGT CGACGTCAGC TTGTCCGAAA ACGTACACTA 
CGCCGCCTGC TTCAAGAGAG AGAGCTCGTG GAACCTCTCA CACCCAGCGG AGAAGCTCCG 
AACCAAGCCC ACTTGAGGAT ATTAAAGGAA ACAGAATTCA AAAAGATCAA AGTTCTGGGT 
TCAGGAGCAT TTGGCACAGT GTATAAGGGT CTCTGGATCC CAGAAGGCGA GAAAGTGAAA 
ATCCCTGTGG CCATCAAGGA GTTAAGAGAA GCCACATCTC CCAAAGCCAA CAAGGAAATC 
CTTGATGAAG CCTACGTGAT GGCCAGTGTG GACAACCCTC ATGTATGCCG CCTCCTGGGC 
ATCTGTCTGA CCTCCACTGT CCAGCTCATT ACACAACTCA TGCCCTATGG TTGCCTCCTG 
GACTATGTCC GAGAACATAA GGACAACATT GGCTCCCAGT ACCTACTCAA CTGGTGTGTG 
CAGATTGCAA AGGGCATGAA CTACCTGGAA GACCGGCGTT TGGTACACCG TGACTTGGCA 
GCCAGGAATG TACTGGTAAA GACACCACAG CATGTCAAGA TCACAGATTT TGGACTGGCC 
AAACTGCTTG GTGCTGAGGA GAAAGAATAC CATGCAGAGG GGGGCAAAGT GCCTATCAAG 
TGGATGGCTT TGGAATCAAT TTTACACCGA ATTTATACAC ACCAAAGCGA CGTCTGGAGC 
TATGGAGTCA CCGTGTGGGA ACTGATGACC TTTGGGTCCA AGCCTTATGA TGGGATCCCT 
GCAAGTGAGA TCTCATCCAT CCTAGAGAAA GGAGAGCGCC TTCCACAGCC ACCTATCTGC 
ACCATCGACG TCTACATGAT CATGGTCAAG TGCTGGATGA TAGATGCTGA TAGCCGCCCA 
AAGTTCCGAG AGTTGATTCT CGAATTCTCC AAAATGGCCA GAGACCCACA GCGCTACCTT 
GTTATCCAGG GGGATGAAAG GATGCATTTG CCGAGCCCTA CAGACTCCAA CTTTTACCGA 
GCCCTGATGG AGGAGGAGGA CATGGAAGAC GTAGTTGATG CTGATGAATA CCTCATCCCA 
CAGCAAGGCT TCTTCAACAG CCCATCCACG TCACGGACTC CACTCTTGAG CTCTCTGAGT 
GCAAATAGCA ACAGTTCCAC TGTGGCTTGC ATTAATAGAA ATGGGAGCTG CCGTGTCAAA 
GAAGACGCCT TCTTGCAACG GTATAGCTCC GATCCCACCA GCGTCCTGAC AGAGGACAAC 
ATAGATGACA CATTCCTTCC CGTGCCTGAA TATATAAACC AATCTGTTCC CAAGAGGCCG 
GCTGGCTCTG TGCAGAACCC AGTCTATCAC AATCAGCCCC TGCATCCAGC TCCTGGAAGA 
GACCTGCATT ATCAAAATCC CCATAGCAAT GCGGTGAGCA ACCCTGAGTA TCTCAACACT 
GCCCAGCCGA CCTGCCTCAG TAGTGGGTTT GACAGCTCTG CCCTCTGGAT CCAGAAAGGC 
AGCCACCAAA TGAGCCTGGA CAACCCTGAC TACCAGCAGG ACTTCTTTCC CAAAGAAGCC 
AAGCCGAATG GCATCTTTAA GGGCCCCACA GCTGAAAATG CAGAGTACCT GCGGGTGGCA 
CCGCCAAGCA GTGAGTTTAG TGGAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113695.1
CDS	154..3783
Translation	MRPSGTARTKLLLLLAALCAAGGALEEKKVCQGTSNRLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAVLSNYGTNKTGLRELPMRNLQEILIGAVRFSNNPILCNMETIQWRDIVQDVFLSNMSMDVQRHLTGCPKCDPSCPNGSCWGRGEENCQKLTKIICAQQCSRRCRGRSPSDCCHNQCAAGCTGPRESDCLVCHRFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGVSKCKKCDGPCRKVCNGIGIGEFKDTLSINATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPENWTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKIMNNRAEKDCKATNHVCNPLCSSEGCWGPEPTDCVSCQNVSRGRECVDKCNILEGEPREFVENSECIQCHPECLPQTMNITCTGRGPDNCIKCAHYVDGPHCVKTCPSGIMGENNTLVWKFADANNVCHLCHANCTYGCAGPGLKGCQQPEGPKIPSIATGIVGGLLFIVVVALGIGLFMRRRQLVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELILEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMEEEDMEDVVDADEYLIPQQGFFNSPSTSRTPLLSSLSANSNSSTVACINRNGSCRVKEDAFLQRYSSDPTSVLTEDNIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLHPAPGRDLHYQNPHSNAVSNPEYLNTAQPTCLSSGFDSSALWIQKGSHQMSLDNPDYQQDFFPKEAKPNGIFKGPTAENAEYLRVAPPSSEFSGA

Target ORF information:

RefSeq Version	NM_031507.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus epidermal growth factor receptor (Egfr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031507.1

ORF Insert Sequence:

ATGCGACCCT CAGGGACTGC GAGAACCAAG CTACTGCTGC TGCTGGCTGC GCTCTGCGCC 
GCAGGTGGGG CGCTGGAGGA AAAGAAAGTT TGCCAAGGCA CAAGTAACAG GCTCACCCAA 
CTAGGCACCT TTGAAGACCA CTTTCTGAGC CTCCAGAGGA TGTTCAACAA CTGTGAAGTG 
GTCCTTGGAA ACTTGGAAAT CACCTATGTG CAAAGGAATT ATGACCTTTC CTTCTTAAAG 
ACCATCCAGG AGGTGGCTGG CTATGTTCTC ATTGCCCTGA ACACCGTGGA GAGAATCCCT 
TTGGAGAACC TGCAGATCAT CAGGGGAAAT GCTCTCTACG AAAACACCTA CGCCTTAGCC 
GTCCTGTCCA ACTATGGAAC CAACAAAACT GGGCTTAGGG AACTGCCCAT GCGGAACTTA 
CAGGAAATTC TGATCGGTGC TGTGCGATTT AGCAACAACC CCATCCTCTG CAATATGGAG 
ACCATCCAGT GGAGGGACAT CGTCCAAGAT GTCTTTCTGA GCAACATGTC AATGGACGTA 
CAGCGCCACC TGACGGGCTG CCCGAAATGT GATCCGAGCT GTCCCAATGG AAGCTGCTGG 
GGAAGAGGAG AGGAGAACTG CCAGAAATTG ACCAAAATCA TCTGCGCCCA GCAATGTTCC 
CGGCGTTGTC GTGGCAGGTC CCCTAGCGAC TGCTGCCACA ACCAGTGTGC CGCAGGGTGT 
ACAGGGCCCA GAGAGAGTGA CTGTCTGGTC TGCCACAGGT TCCGAGATGA AGCCACGTGC 
AAAGACACCT GCCCACCACT CATGCTGTAC AACCCCACCA CGTACCAGAT GGATGTCAAC 
CCTGAGGGGA AGTACAGCTT TGGTGCCACC TGTGTGAAGA AATGCCCCAG AAACTACGTG 
GTGACAGATC ACGGCTCGTG TGTCCGGGCC TGTGGGCCAG ACTACTATGA AGTAGAAGAA 
GATGGAGTCA GCAAGTGTAA AAAATGTGAC GGGCCCTGCC GCAAAGTTTG CAATGGCATA 
GGCATTGGTG AATTTAAAGA CACACTCTCC ATAAATGCTA CAAACATCAA ACACTTCAAG 
TACTGCACTG CCATCAGTGG GGACCTCCAC ATCCTGCCAG TGGCCTTTAA GGGGGATTCT 
TTCACCCGCA CTCCTCCTCT AGACCCACGG GAACTAGAAA TTCTCAAAAC TGTGAAGGAA 
ATAACAGGGT TTTTGCTGAT TCAGGCTTGG CCTGAAAACT GGACTGACCT CCATGCTTTT 
GAGAACCTAG AAATAATTCG TGGCAGAACA AAGCAACATG GTCAGTTTTC TCTGGCGGTT 
GTCGGCCTGA ACATAACATC GCTGGGGTTG CGTTCCCTCA AGGAGATCAG TGATGGGGAT 
GTGATTATTT CTGGGAACCG AAATTTGTGC TACGCAAACA CTATAAACTG GAAAAAACTC 
TTCGGGACGC CCAATCAAAA GACCAAAATC ATGAACAACA GAGCTGAAAA GGACTGCAAG 
GCCACGAACC ACGTCTGTAA TCCTTTATGC TCCTCGGAAG GCTGCTGGGG CCCTGAGCCC 
ACGGACTGTG TCTCCTGCCA GAATGTGAGC AGAGGCAGGG AGTGCGTGGA CAAGTGCAAC 
ATCCTGGAGG GGGAACCGAG GGAGTTTGTG GAAAATTCTG AATGCATCCA GTGCCATCCA 
GAATGTCTGC CCCAGACCAT GAACATCACC TGTACAGGCC GGGGGCCAGA CAACTGCATC 
AAGTGTGCCC ACTATGTTGA TGGTCCCCAC TGTGTCAAGA CCTGCCCTTC GGGCATCATG 
GGGGAGAACA ACACCCTGGT CTGGAAGTTT GCAGATGCCA ATAACGTCTG CCACCTCTGC 
CATGCAAACT GTACCTATGG ATGTGCTGGG CCAGGCCTTA AAGGATGTCA ACAACCAGAA 
GGGCCAAAGA TCCCATCCAT CGCCACTGGG ATTGTGGGTG GCCTCCTCTT CATAGTAGTG 
GTGGCCCTTG GGATCGGCCT CTTCATGCGT CGACGTCAGC TTGTCCGAAA ACGTACACTA 
CGCCGCCTGC TTCAAGAGAG AGAGCTCGTG GAACCTCTCA CACCCAGCGG AGAAGCTCCG 
AACCAAGCCC ACTTGAGGAT ATTAAAGGAA ACAGAATTCA AAAAGATCAA AGTTCTGGGT 
TCAGGAGCAT TTGGCACAGT GTATAAGGGT CTCTGGATCC CAGAAGGCGA GAAAGTGAAA 
ATCCCTGTGG CCATCAAGGA GTTAAGAGAA GCCACATCTC CCAAAGCCAA CAAGGAAATC 
CTTGATGAAG CCTACGTGAT GGCCAGTGTG GACAACCCTC ATGTATGCCG CCTCCTGGGC 
ATCTGTCTGA CCTCCACTGT CCAGCTCATT ACACAACTCA TGCCCTATGG TTGCCTCCTG 
GACTATGTCC GAGAACATAA GGACAACATT GGCTCCCAGT ACCTACTCAA CTGGTGTGTG 
CAGATTGCAA AGGGCATGAA CTACCTGGAA GACCGGCGTT TGGTACACCG TGACTTGGCA 
GCCAGGAATG TACTGGTAAA GACACCACAG CATGTCAAGA TCACAGATTT TGGACTGGCC 
AAACTGCTTG GTGCTGAGGA GAAAGAATAC CATGCAGAGG GGGGCAAAGT GCCTATCAAG 
TGGATGGCTT TGGAATCAAT TTTACACCGA ATTTATACAC ACCAAAGCGA CGTCTGGAGC 
TATGGAGTCA CCGTGTGGGA ACTGATGACC TTTGGGTCCA AGCCTTATGA TGGGATCCCT 
GCAAGTGAGA TCTCATCCAT CCTAGAGAAA GGAGAGCGCC TTCCACAGCC ACCTATCTGC 
ACCATCGACG TCTACATGAT CATGGTCAAG TGCTGGATGA TAGATGCTGA TAGCCGCCCA 
AAGTTCCGAG AGTTGATTCT CGAATTCTCC AAAATGGCCA GAGACCCACA GCGCTACCTT 
GTTATCCAGG GGGATGAAAG GATGCATTTG CCGAGCCCTA CAGACTCCAA CTTTTACCGA 
GCCCTGATGG AGGAGGAGGA CATGGAAGAC GTAGTTGATG CTGATGAATA CCTCATCCCA 
CAGCAAGGCT TCTTCAACAG CCCATCCACG TCACGGACTC CACTCTTGAG CTCTCTGAGT 
GCAAATAGCA ACAGTTCCAC TGTGGCTTGC ATTAATAGAA ATGGGAGCTG CCGTGTCAAA 
GAAGACGCCT TCTTGCAACG GTATAGCTCC GATCCCACCA GCGTCCTGAC AGAGGACAAC 
ATAGATGACA CATTCCTTCC CGTGCCTGAA TATATAAACC AATCTGTTCC CAAGAGGCCG 
GCTGGCTCTG TGCAGAACCC AGTCTATCAC AATCAGCCCC TGCATCCAGC TCCTGGAAGA 
GACCTGCATT ATCAAAATCC CCATAGCAAT GCGGTGAGCA ACCCTGAGTA TCTCAACACT 
GCCCAGCCGA CCTGCCTCAG TAGTGGGTTT GACAGCTCTG CCCTCTGGAT CCAGAAAGGC 
AGCCACCAAA TGAGCCTGGA CAACCCTGAC TACCAGCAGG ACTTCTTTCC CAAAGAAGCC 
AAGCCGAATG GCATCTTTAA GGGCCCCACA GCTGAAAATG CAGAGTACCT GCGGGTGGCA 
CCGCCAAGCA GTGAGTTTAG TGGAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egfr ( XM_008770416.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768638.1 Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X1, mRNA.

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CloneID	ORa42894
Clone ID Related Accession (Same CDS sequence)	XM_008770416.2
Accession Version	XM_008770416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	epidermal growth factor receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCAGT TATGCAACCG TTGCTGTTGT GTCCATCCAT GGAGAATAAT TAACAGAGCA 
GCCATTTCCG ATTTCACTGA TCTCGACCTG GATGGTTTTA CGGGACATCC TGTGTTGCTT 
TGTTATATTG GACGTGCAGG CAGAGAGCAC GCTGGGTCTT CTCGGAATCC ATTCTCCCAC 
TCAGGAGTTT GCCAAGGCAC AAGTAACAGG CTCACCCAAC TAGGCACCTT TGAAGACCAC 
TTTCTGAGCC TCCAGAGGAT GTTCAACAAC TGTGAAGTGG TCCTTGGAAA CTTGGAAATC 
ACCTATGTGC AAAGGAATTA TGACCTTTCC TTCTTAAAGA CCATTCAGGA GGTGGCTGGC 
TATGTTCTCA TTGCCCTGAA CACCGTGGAG AGAATCCCTT TGGAGAACCT GCAGATCATC 
AGGGGAAATG CTCTCTACGA AAACACCTAC GCCTTAGCCG TCCTGTCCAA CTATGGAACC 
AACAAAACTG GGCTTAGGGA ACTGCCCATG CGGAACTTAC AGGAAATTCT GATCGGTGCT 
GTGCGATTTA GCAACAACCC CATCCTCTGC AATATGGAGA CCATCCAGTG GAGGGACATC 
GTCCAAGATG TCTTTCTGAG CAACATGTCA ATGGACGTAC AGCGCCACCT GACCGGCTGC 
CCGAAATGTG ATCCGAGCTG TCCCAATGGA AGCTGCTGGG GAAGAGGAGA GGAGAACTGC 
CAGAAATTGA CCAAAATCAT CTGCGCCCAG CAATGTTCCC GGCGTTGTCG TGGCAGGTCC 
CCTAGCGACT GCTGCCACAA CCAGTGTGCC GCAGGGTGTA CAGGGCCCAG AGAGAGTGAC 
TGTCTGGTCT GCCACAGGTT CCGAGATGAA GCCACGTGCA AAGACACCTG CCCACCACTC 
ATGCTGTACA ACCCCACCAC GTACCAGATG GATGTCAACC CTGAGGGGAA GTACAGCTTT 
GGTGCCACCT GTGTGAAGAA ATGCCCCAGA AACTACGTGG TGACAGATCA CGGCTCGTGT 
GTCCGGGCCT GTGGGCCAGA CTACTATGAA GTAGAAGAAG ATGGAGTCAG CAAGTGTAAA 
AAATGTGATG GGCCCTGCCG CAAAGTTTGC AATGGCATAG GCATTGGTGA ATTTAAAGAC 
ACACTCTCCA TAAATGCTAC AAACATCAAA CACTTCAAGT ACTGCACTGC CATCAGTGGG 
GACCTCCACA TCCTGCCAGT GGCCTTTAAG GGGGATTCTT TCACCCGCAC TCCTCCTCTA 
GACCCACGGG AACTAGAAAT TCTCAAAACT GTGAAGGAAA TAACAGGGTT TTTGCTGATT 
CAGGCTTGGC CTGAAAACTG GACTGACCTC CATGCTTTTG AGAACCTAGA AATAATTCGT 
GGCAGAACAA AGCAACATGG TCAGTTTTCT CTGGCGGTTG TCGGCCTGAA CATAACATCG 
CTGGGGTTGC GTTCCCTCAA GGAGATCAGT GATGGGGATG TGATTATTTC TGGGAACCGA 
AATTTGTGCT ACGCAAACAC TATAAACTGG AAAAAACTCT TCGGGACGCC CAATCAAAAG 
ACCAAAATCA TGAACAACAG AGCTGAAAAG GACTGCAAGG CCACGAACCA CGTCTGTAAT 
CCTTTATGCT CCTCGGAAGG CTGCTGGGGC CCTGAGCCCA CGGACTGTGT CTCCTGCCAG 
AATGTGAGCA GAGGCAGGGA GTGCGTGGAC AAGTGCAACA TCCTGGAGGG GGAACCGAGG 
GAGTTTGTGG AAAATTCTGA ATGCATCCAG TGCCATCCAG AATGTCTGCC CCAGACCATG 
AACATCACCT GTACAGGCCG GGGGCCAGAC AACTGCATCA AGTGTGCCCA CTATGTTGAT 
GGTCCCCACT GTGTCAAGAC CTGCCCTTCG GGCATCATGG GGGAGAACAA CACCCTGGTC 
TGGAAGTTTG CAGATGCCAA TAACGTCTGC CACCTCTGCC ATGCAAACTG TACCTATGGA 
TGTGCTGGGC CAGGCCTTAA AGGATGTCAA CAACCAGAAG GGCCAAAGAT CCCATCCATC 
GCCACTGGGA TTGTGGGTGG CCTCCTCTTC ATAGTAGTGG TGGCCCTTGG GATCGGCCTC 
TTCATGCGAA GGCGTCACAT TGTCCGAAAA CGTACACTAC GCCGCCTGCT TCAAGAGAGA 
GAGCTCGTGG AACCTCTCAC ACCCAGCGGA GAAGCTCCGA ACCAAGCCCA CTTGAGGATA 
TTAAAGGAAA CAGAATTCAA AAAGATCAAA GTTCTGGGTT CAGGAGCATT TGGCACAGTG 
TATAAGGGTC TCTGGATCCC AGAAGGCGAG AAAGTGAAAA TCCCTGTGGC CATCAAGGAG 
TTAAGAGAAG CCACATCTCC CAAAGCCAAC AAGGAAATCC TTGATGAAGC CTACGTGATG 
GCCAGTGTGG ACAACCCTCA TGTATGCCGC CTCCTGGGCA TCTGTCTGAC CTCCACTGTC 
CAGCTCATTA CACAACTCAT GCCCTATGGT TGCCTCCTGG ACTATGTCCG AGAACATAAG 
GACAACATTG GCTCCCAGTA CCTACTCAAC TGGTGTGTGC AGATTGCAAA GGGCATGAAC 
TACCTGGAAG ACCGGCGTTT GGTACACCGT GACTTGGCAG CCAGGAATGT ACTGGTAAAG 
ACACCACAGC ATGTCAAGAT CACAGATTTT GGACTGGCCA AACTGCTTGG TGCTGAGGAG 
AAAGAATACC ATGCAGAGGG GGGCAAAGTG CCTATCAAGT GGATGGCTTT GGAATCAATT 
TTACACCGAA TTTATACACA CCAAAGCGAC GTCTGGAGCT ATGGAGTCAC CGTGTGGGAA 
CTGATGACCT TTGGGTCCAA GCCTTATGAT GGGATCCCTG CAAGTGAGAT CTCATCCATC 
CTAGAGAAAG GAGAGCGCCT TCCACAGCCA CCTATCTGCA CCATCGACGT CTACATGATC 
ATGGTCAAGT GCTGGATGAT AGATGCTGAT AGCCGCCCAA AGTTCCGAGA GTTGATTCTC 
GAATTCTCCA AAATGGCCAG AGACCCACAG CGCTACCTTG TTATCCAGGG GGATGAAAGG 
ATGCATTTGC CGAGCCCTAC AGACTCCAAC TTTTACCGAG CCCTGATGGA GGAGGAGGAC 
ATGGAAGACG TAGTTGATGC TGATGAATAC CTCATCCCAC AGCAAGGCTT CTTCAACAGC 
CCATCCACGT CACGGACTCC ACTCTTGAGC TCTCTGAGTG CAAATAGCAA CAGTTCCGCT 
GTGGCTTGCA TTAATAGAAA TGGGAGCTGC CGTGTCAAAG AAGACGCCTT CTTGCAACGG 
TATAGCTCCG ATCCCACCAG CGTCCTGACA GAGGACAACA TAGATGACAC ATTCCTTCCC 
GTGCCTGAAT ATATAAACCA ATCTGTTCCC AAGAGGCCGG CTGGCTCTGT GCAGAACCCA 
GTCTATCACA ATCAGCCCCT GCATCCAGCT CCTGGAAGAG ACCTGCATTA TCAAAATCCC 
CATAGCAATG CGGTGAGCAA CCCTGAGTAT CTCAACACTG CCCAGCCGAC CTGCCTCAGT 
AGTGGGTTTG ACAGCTCTGC CCTCTGGATC CAGAAAGGCA GCCACCAAAT GAGCCTGGAC 
AACCCTGACT ACCAGCAGGA CTTCTTTCCC AAAGAAGCCA AGCCGAATGG CATCTTTAAG 
GGCCCCACAG CTGAAAATGC AGAGTACCTG CGGGTGGCAC CGCCAAGCAG TGAGTTTATT 
GGAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768638.1
CDS	8910..12638
Translation	MFQLCNRCCCVHPWRIINRAAISDFTDLDLDGFTGHPVLLCYIGRAGREHAGSSRNPFSHSGVCQGTSNRLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNALYENTYALAVLSNYGTNKTGLRELPMRNLQEILIGAVRFSNNPILCNMETIQWRDIVQDVFLSNMSMDVQRHLTGCPKCDPSCPNGSCWGRGEENCQKLTKIICAQQCSRRCRGRSPSDCCHNQCAAGCTGPRESDCLVCHRFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGPDYYEVEEDGVSKCKKCDGPCRKVCNGIGIGEFKDTLSINATNIKHFKYCTAISGDLHILPVAFKGDSFTRTPPLDPRELEILKTVKEITGFLLIQAWPENWTDLHAFENLEIIRGRTKQHGQFSLAVVGLNITSLGLRSLKEISDGDVIISGNRNLCYANTINWKKLFGTPNQKTKIMNNRAEKDCKATNHVCNPLCSSEGCWGPEPTDCVSCQNVSRGRECVDKCNILEGEPREFVENSECIQCHPECLPQTMNITCTGRGPDNCIKCAHYVDGPHCVKTCPSGIMGENNTLVWKFADANNVCHLCHANCTYGCAGPGLKGCQQPEGPKIPSIATGIVGGLLFIVVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQAHLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPYGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELILEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMEEEDMEDVVDADEYLIPQQGFFNSPSTSRTPLLSSLSANSNSSAVACINRNGSCRVKEDAFLQRYSSDPTSVLTEDNIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLHPAPGRDLHYQNPHSNAVSNPEYLNTAQPTCLSSGFDSSALWIQKGSHQMSLDNPDYQQDFFPKEAKPNGIFKGPTAENAEYLRVAPPSSEFIGA

Target ORF information:

RefSeq Version	XM_008770416.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus epidermal growth factor receptor (Egfr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770416.2

ORF Insert Sequence:

ATGTTCCAGT TATGCAACCG TTGCTGTTGT GTCCATCCAT GGAGAATAAT TAACAGAGCA 
GCCATTTCCG ATTTCACTGA TCTCGACCTG GATGGTTTTA CGGGACATCC TGTGTTGCTT 
TGTTATATTG GACGTGCAGG CAGAGAGCAC GCTGGGTCTT CTCGGAATCC ATTCTCCCAC 
TCAGGAGTTT GCCAAGGCAC AAGTAACAGG CTCACCCAAC TAGGCACCTT TGAAGACCAC 
TTTCTGAGCC TCCAGAGGAT GTTCAACAAC TGTGAAGTGG TCCTTGGAAA CTTGGAAATC 
ACCTATGTGC AAAGGAATTA TGACCTTTCC TTCTTAAAGA CCATTCAGGA GGTGGCTGGC 
TATGTTCTCA TTGCCCTGAA CACCGTGGAG AGAATCCCTT TGGAGAACCT GCAGATCATC 
AGGGGAAATG CTCTCTACGA AAACACCTAC GCCTTAGCCG TCCTGTCCAA CTATGGAACC 
AACAAAACTG GGCTTAGGGA ACTGCCCATG CGGAACTTAC AGGAAATTCT GATCGGTGCT 
GTGCGATTTA GCAACAACCC CATCCTCTGC AATATGGAGA CCATCCAGTG GAGGGACATC 
GTCCAAGATG TCTTTCTGAG CAACATGTCA ATGGACGTAC AGCGCCACCT GACCGGCTGC 
CCGAAATGTG ATCCGAGCTG TCCCAATGGA AGCTGCTGGG GAAGAGGAGA GGAGAACTGC 
CAGAAATTGA CCAAAATCAT CTGCGCCCAG CAATGTTCCC GGCGTTGTCG TGGCAGGTCC 
CCTAGCGACT GCTGCCACAA CCAGTGTGCC GCAGGGTGTA CAGGGCCCAG AGAGAGTGAC 
TGTCTGGTCT GCCACAGGTT CCGAGATGAA GCCACGTGCA AAGACACCTG CCCACCACTC 
ATGCTGTACA ACCCCACCAC GTACCAGATG GATGTCAACC CTGAGGGGAA GTACAGCTTT 
GGTGCCACCT GTGTGAAGAA ATGCCCCAGA AACTACGTGG TGACAGATCA CGGCTCGTGT 
GTCCGGGCCT GTGGGCCAGA CTACTATGAA GTAGAAGAAG ATGGAGTCAG CAAGTGTAAA 
AAATGTGATG GGCCCTGCCG CAAAGTTTGC AATGGCATAG GCATTGGTGA ATTTAAAGAC 
ACACTCTCCA TAAATGCTAC AAACATCAAA CACTTCAAGT ACTGCACTGC CATCAGTGGG 
GACCTCCACA TCCTGCCAGT GGCCTTTAAG GGGGATTCTT TCACCCGCAC TCCTCCTCTA 
GACCCACGGG AACTAGAAAT TCTCAAAACT GTGAAGGAAA TAACAGGGTT TTTGCTGATT 
CAGGCTTGGC CTGAAAACTG GACTGACCTC CATGCTTTTG AGAACCTAGA AATAATTCGT 
GGCAGAACAA AGCAACATGG TCAGTTTTCT CTGGCGGTTG TCGGCCTGAA CATAACATCG 
CTGGGGTTGC GTTCCCTCAA GGAGATCAGT GATGGGGATG TGATTATTTC TGGGAACCGA 
AATTTGTGCT ACGCAAACAC TATAAACTGG AAAAAACTCT TCGGGACGCC CAATCAAAAG 
ACCAAAATCA TGAACAACAG AGCTGAAAAG GACTGCAAGG CCACGAACCA CGTCTGTAAT 
CCTTTATGCT CCTCGGAAGG CTGCTGGGGC CCTGAGCCCA CGGACTGTGT CTCCTGCCAG 
AATGTGAGCA GAGGCAGGGA GTGCGTGGAC AAGTGCAACA TCCTGGAGGG GGAACCGAGG 
GAGTTTGTGG AAAATTCTGA ATGCATCCAG TGCCATCCAG AATGTCTGCC CCAGACCATG 
AACATCACCT GTACAGGCCG GGGGCCAGAC AACTGCATCA AGTGTGCCCA CTATGTTGAT 
GGTCCCCACT GTGTCAAGAC CTGCCCTTCG GGCATCATGG GGGAGAACAA CACCCTGGTC 
TGGAAGTTTG CAGATGCCAA TAACGTCTGC CACCTCTGCC ATGCAAACTG TACCTATGGA 
TGTGCTGGGC CAGGCCTTAA AGGATGTCAA CAACCAGAAG GGCCAAAGAT CCCATCCATC 
GCCACTGGGA TTGTGGGTGG CCTCCTCTTC ATAGTAGTGG TGGCCCTTGG GATCGGCCTC 
TTCATGCGAA GGCGTCACAT TGTCCGAAAA CGTACACTAC GCCGCCTGCT TCAAGAGAGA 
GAGCTCGTGG AACCTCTCAC ACCCAGCGGA GAAGCTCCGA ACCAAGCCCA CTTGAGGATA 
TTAAAGGAAA CAGAATTCAA AAAGATCAAA GTTCTGGGTT CAGGAGCATT TGGCACAGTG 
TATAAGGGTC TCTGGATCCC AGAAGGCGAG AAAGTGAAAA TCCCTGTGGC CATCAAGGAG 
TTAAGAGAAG CCACATCTCC CAAAGCCAAC AAGGAAATCC TTGATGAAGC CTACGTGATG 
GCCAGTGTGG ACAACCCTCA TGTATGCCGC CTCCTGGGCA TCTGTCTGAC CTCCACTGTC 
CAGCTCATTA CACAACTCAT GCCCTATGGT TGCCTCCTGG ACTATGTCCG AGAACATAAG 
GACAACATTG GCTCCCAGTA CCTACTCAAC TGGTGTGTGC AGATTGCAAA GGGCATGAAC 
TACCTGGAAG ACCGGCGTTT GGTACACCGT GACTTGGCAG CCAGGAATGT ACTGGTAAAG 
ACACCACAGC ATGTCAAGAT CACAGATTTT GGACTGGCCA AACTGCTTGG TGCTGAGGAG 
AAAGAATACC ATGCAGAGGG GGGCAAAGTG CCTATCAAGT GGATGGCTTT GGAATCAATT 
TTACACCGAA TTTATACACA CCAAAGCGAC GTCTGGAGCT ATGGAGTCAC CGTGTGGGAA 
CTGATGACCT TTGGGTCCAA GCCTTATGAT GGGATCCCTG CAAGTGAGAT CTCATCCATC 
CTAGAGAAAG GAGAGCGCCT TCCACAGCCA CCTATCTGCA CCATCGACGT CTACATGATC 
ATGGTCAAGT GCTGGATGAT AGATGCTGAT AGCCGCCCAA AGTTCCGAGA GTTGATTCTC 
GAATTCTCCA AAATGGCCAG AGACCCACAG CGCTACCTTG TTATCCAGGG GGATGAAAGG 
ATGCATTTGC CGAGCCCTAC AGACTCCAAC TTTTACCGAG CCCTGATGGA GGAGGAGGAC 
ATGGAAGACG TAGTTGATGC TGATGAATAC CTCATCCCAC AGCAAGGCTT CTTCAACAGC 
CCATCCACGT CACGGACTCC ACTCTTGAGC TCTCTGAGTG CAAATAGCAA CAGTTCCGCT 
GTGGCTTGCA TTAATAGAAA TGGGAGCTGC CGTGTCAAAG AAGACGCCTT CTTGCAACGG 
TATAGCTCCG ATCCCACCAG CGTCCTGACA GAGGACAACA TAGATGACAC ATTCCTTCCC 
GTGCCTGAAT ATATAAACCA ATCTGTTCCC AAGAGGCCGG CTGGCTCTGT GCAGAACCCA 
GTCTATCACA ATCAGCCCCT GCATCCAGCT CCTGGAAGAG ACCTGCATTA TCAAAATCCC 
CATAGCAATG CGGTGAGCAA CCCTGAGTAT CTCAACACTG CCCAGCCGAC CTGCCTCAGT 
AGTGGGTTTG ACAGCTCTGC CCTCTGGATC CAGAAAGGCA GCCACCAAAT GAGCCTGGAC 
AACCCTGACT ACCAGCAGGA CTTCTTTCCC AAAGAAGCCA AGCCGAATGG CATCTTTAAG 
GGCCCCACAG CTGAAAATGC AGAGTACCTG CGGGTGGCAC CGCCAAGCAG TGAGTTTATT 
GGAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Src-mediated ligand release-independent EGFR transactivation involves TGF-?-induced Smad3 activation in mesangial cells.
Biochemical and biophysical research communications493(2)914-920(2017 11)
Chen Y,Peng FF,Jin J,Chen HM,Yu H,Zhang BF

Noise Stress Induces an Epidermal Growth Factor Receptor/Xeroderma Pigmentosum-A Response in the Auditory Nerve.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society65(3)173-184(2017 Mar)
Guthrie OW

Radiolabeled novel mAb 4G1 for immunoSPECT imaging of EGFRvIII expression in preclinical glioblastoma xenografts.
Oncotarget8(4)6364-6375(2017 Jan)
Liu X,Dong C,Shi J,Ma T,Jin Z,Jia B,Liu Z,Shen L,Wang F

Epidermal Growth Factor Receptor Silencing Blunts the Slow Force Response to Myocardial Stretch.
Journal of the American Heart Association5(10)(2016 Oct)
Brea MS,D?az RG,Escudero DS,Caldiz CI,Portiansky EL,Morgan PE,P?rez NG

Periodic Mechanical Stress Activates PKC?-Dependent EGFR Mitogenic Signals in Rat Chondrocytes via PI3K-Akt and ERK1/2.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology39(4)1281-94(2016)
He P,Shen N,Gao G,Jiang X,Sun H,Zhou D,Xu N,Nong L,Ren K

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Egfr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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