Cyp11b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp11b2
Entrez Gene ID	24294
Full Name	cytochrome P450, family 11, subfamily b, polypeptide 2
Synonyms	Cp45as,Cyp11b3,RNCP45AS
General protein information	Preferred Names cytochrome P450, family 11, subfamily b, polypeptide 2 Names cytochrome P450 11B2, mitochondrial CYPXIB2 CYPXIB3 Cytochrome P450, subfamily XIB, polypeptide 2 (aldosterone synthase) P450-Aldo-1 P450-Aldo-2 aldosterone synthase cytochrome P450 11B3, mitochondrial cytochrome P450, subfamily 11B, polypeptide 2 cytochrome P450-Aldo-1 cytochrome P450-Aldo-2 steroid 11-beta-hydroxylase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34
Summary	steroid 11-beta-monooxygenase that catalyzes steps in the biosynthesis of aldosterone, corticosterone, and 18-hydroxycorticosterone [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765543.2	XP_008763765.1	cytochrome P450 11B2, mitochondrial isoform X4
XM_008765542.2	XP_008763764.1	cytochrome P450 11B2, mitochondrial isoform X3
XM_008765540.2	XP_008763762.1	cytochrome P450 11B2, mitochondrial isoform X1
XM_008765541.2	XP_008763763.1	cytochrome P450 11B2, mitochondrial isoform X2
NM_012538.2	NP_036670.2	cytochrome P450 11B2, mitochondrial

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cyp11c1	NP_001073673.1
Pan troglodytes (chimpanzee)	LOC464437	XP_519994.3
	CYP11B1	XP_001084565.1
Mus musculus (house mouse)	Cyp11b2	NP_034121.3
Rattus norvegicus (Norway rat)	Cyp11b2	NP_036670.2
Homo sapiens (human)	CYP11B1	NP_000488.3
Xenopus tropicalis (tropical clawed frog)	LOC101731070	XP_004920081.1

Gene Ontology
Bibliography

Related articles in PubMed

Aldosterone Synthase CYP11B2 Gene Promoter Polymorphism in a Turkish Population With Chronic Kidney Disease.
Yilmaz M, Sari I, Bagci B, Gumus E, Ozdemir O
Iranian journal of kidney diseases9(3)209-14(2015 May)

[The influence of suoquan capsule on the mRNA expression of CYP11B2 in deficiency of the kidney and diuresis rats].
Li SW, Hu LJ, Wu QH, Huang P, Cao HY
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials34(1)80-3(2011 Jan)

Sodium depletion increases sympathetic neurite outgrowth and expression of a novel TMEM35 gene-derived protein (TUF1) in the rat adrenal zona glomerulosa.
Tran PV, Georgieff MK, Engeland WC
Endocrinology151(10)4852-60(2010 Oct)

In vivo regulation of gene expression of enzymes controlling aldosterone synthesis in rat adrenal.
LeHoux JG, Tremblay A
The Journal of steroid biochemistry and molecular biology43(8)837-46(1992 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data showed that that the CYP11B2 gene -344 C>T polymorphism may have an effect on the development of CKD in diabetic patients.
Title: Aldosterone Synthase CYP11B2 Gene Promoter Polymorphism in a Turkish Population With Chronic Kidney Disease.

Suoquan capsule can increase the mRNA expression of CYP11B2 in rats with kidney deficiency and diuresis.
Title: [The influence of suoquan capsule on the mRNA expression of CYP11B2 in deficiency of the kidney and diuresis rats].

Role of central nervous system aldosterone synthase and mineralocorticoid receptors in salt-induced hypertension in Dahl salt-sensitive rats.
Title: Role of central nervous system aldosterone synthase and mineralocorticoid receptors in salt-induced hypertension in Dahl salt-sensitive rats.

Ang II increased the expression levels of early transcription factors NR4A1 and NR4A2 and aldosterone synthase
Title: Mechanisms of angiotensin II-mediated regulation of aldosterone synthase expression in H295R human adrenocortical and rat adrenal glomerulosa cells.

Data suggest that variations in intake of sodium and potassium act on expression of CYP11B2 in adrenal zona glomerulosa, but not on expression of CYP11B1.
Title: In vivo regulation of gene expression of enzymes controlling aldosterone synthesis in rat adrenal.

The following Cyp11b2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp11b2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12153	NM_012538.2 Latest version!	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa42881	XM_008765540.2 Latest version!	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42882	XM_008765541.2 Latest version!	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42883	XM_008765542.2 Latest version!	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa42884	XM_008765543.2 Latest version!	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp11b2 ( NM_012538.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036670.2 Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), mRNA.

Price & Availability↑

CloneID	ORa12153
Clone ID Related Accession (Same CDS sequence)	NM_012538.2
Accession Version	NM_012538.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 11B2, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07058434.1. On Apr 15, 2009 this sequence version replaced NM_012538.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52766.1, D00568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642898 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGACGT TGGTCCTACT TTATCTGTAC TCCATGGGCC GAAACCCTGC AGTGTTCCCA 
AGACCTGAGC GCTATATGCC TCAGCGCTGG CTGGAGAGGA AAAGGAGTTT CCAGCATCTG 
GCCTTCGGCT TTGGGGTGCG CCAGTGCCTG GGGCGGCGCC TGGCAGAGGT GGAGATGCTG 
CTCCTGCTTC ACCATATGCT GAAAACCTTC CAGGTGGAGA CACTGAGACA AGAGGATGTG 
CAGATGGCCT ATCGCTTTGT TTTGATGCCC AGCTCTAGTC CTGTCCTCAC TTTCCGGCCC 
GTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036670.2
CDS	1..1509
Translation	MAMALRVTADVWLARPWQCLHRTRALGTTATLAPKTLKPFEAIPQYSRNKWLKMIQILREQGQENLHLEMHQAFQELGPIFRHSAGGAQIVSVMLPEDAEKLHQVESILPRRMHLEPWVAHRELRGLRRGVFLLNGADWRFNRLKLNPNVLSPKAVQNFVPMVDEVARDFLEALKKKVRQNARGSLTMDVQQSLFNYTIEASNFALFGERLGLLGHDLNPGSLKFIHALHSMFKSTTQLLFLPRSLTRWTSTRVWKEHFDAWDVISEYANRCIWKVHQELRLGSSQTYSGIVAALITQGALPLDAIKANSMELTAGSVDTTAIPLVMTLFELARNPDVQQALRQETLAAEASIAANPQKAMSDLPLLRAALKETLRLYPVGGFLERILNSDLVLQNYHVPAGTLVLLYLYSMGRNPAVFPRPERYMPQRWLERKRSFQHLAFGFGVRQCLGRRLAEVEMLLLLHHMLKTFQVETLRQEDVQMAYRFVLMPSSSPVLTFRPVS

Target ORF information:

RefSeq Version	NM_012538.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012538.2

ORF Insert Sequence:

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGACGT TGGTCCTACT TTATCTGTAC TCCATGGGCC GAAACCCTGC AGTGTTCCCA 
AGACCTGAGC GCTATATGCC TCAGCGCTGG CTGGAGAGGA AAAGGAGTTT CCAGCATCTG 
GCCTTCGGCT TTGGGGTGCG CCAGTGCCTG GGGCGGCGCC TGGCAGAGGT GGAGATGCTG 
CTCCTGCTTC ACCATATGCT GAAAACCTTC CAGGTGGAGA CACTGAGACA AGAGGATGTG 
CAGATGGCCT ATCGCTTTGT TTTGATGCCC AGCTCTAGTC CTGTCCTCAC TTTCCGGCCC 
GTCAGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp11b2 ( XM_008765540.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763762.1 Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42881
Clone ID Related Accession (Same CDS sequence)	XM_008765540.2
Accession Version	XM_008765540.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 11B2, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGACGT TGGTCCTACT TTATCTGTAC TCCATGGGCC GAAACCCTGC AGTGTTCCCA 
AGACCTGAGC GCTATATGCC TCAGCGCTGG CTGGAGAGGA AAAGGAGTTT CCAGCATCTG 
GCCTTCGGCT TTGGGGTGCG CCAGTGCCTG GGGCGGCGCC TGGCAGAGGT GGAGATGCTG 
CTCCTGCTTC ACCATGTGAG CACCTCTGGG TGGGCCTATG CTGAAAACCT TCCAGGTGGA 
GACACTGAGA CAAGAGGATG TGCAGATGGC CTATCGCTTT GTTTTGATGC CCAGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763762.1
CDS	33..1532
Translation	MAMALRVTADVWLARPWQCLHRTRALGTTATLAPKTLKPFEAIPQYSRNKWLKMIQILREQGQENLHLEMHQAFQELGPIFRHSAGGAQIVSVMLPEDAEKLHQVESILPRRMHLEPWVAHRELRGLRRGVFLLNGADWRFNRLKLNPNVLSPKAVQNFVPMVDEVARDFLEALKKKVRQNARGSLTMDVQQSLFNYTIEASNFALFGERLGLLGHDLNPGSLKFIHALHSMFKSTTQLLFLPRSLTRWTSTRVWKEHFDAWDVISEYANRCIWKVHQELRLGSSQTYSGIVAALITQGALPLDAIKANSMELTAGSVDTTAIPLVMTLFELARNPDVQQALRQETLAAEASIAANPQKAMSDLPLLRAALKETLRLYPVGGFLERILNSDLVLQNYHVPAGTLVLLYLYSMGRNPAVFPRPERYMPQRWLERKRSFQHLAFGFGVRQCLGRRLAEVEMLLLLHHVSTSGWAYAENLPGGDTETRGCADGLSLCFDAQL

Target ORF information:

RefSeq Version	XM_008765540.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765540.2

ORF Insert Sequence:

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGACGT TGGTCCTACT TTATCTGTAC TCCATGGGCC GAAACCCTGC AGTGTTCCCA 
AGACCTGAGC GCTATATGCC TCAGCGCTGG CTGGAGAGGA AAAGGAGTTT CCAGCATCTG 
GCCTTCGGCT TTGGGGTGCG CCAGTGCCTG GGGCGGCGCC TGGCAGAGGT GGAGATGCTG 
CTCCTGCTTC ACCATGTGAG CACCTCTGGG TGGGCCTATG CTGAAAACCT TCCAGGTGGA 
GACACTGAGA CAAGAGGATG TGCAGATGGC CTATCGCTTT GTTTTGATGC CCAGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp11b2 ( XM_008765541.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763763.1 Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa42882
Clone ID Related Accession (Same CDS sequence)	XM_008765541.2
Accession Version	XM_008765541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 11B2, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765541.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGTGTT CCCAAGACCT GAGCGCTATA TGCCTCAGCG CTGGCTGGAG AGGAAAAGGA 
GTTTCCAGCA TCTGGCCTTC GGCTTTGGGG TGCGCCAGTG CCTGGGGCGG CGCCTGGCAG 
AGGTGGAGAT GCTGCTCCTG CTTCACCATA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763763.1
CDS	33..1388
Translation	MAMALRVTADVWLARPWQCLHRTRALGTTATLAPKTLKPFEAIPQYSRNKWLKMIQILREQGQENLHLEMHQAFQELGPIFRHSAGGAQIVSVMLPEDAEKLHQVESILPRRMHLEPWVAHRELRGLRRGVFLLNGADWRFNRLKLNPNVLSPKAVQNFVPMVDEVARDFLEALKKKVRQNARGSLTMDVQQSLFNYTIEASNFALFGERLGLLGHDLNPGSLKFIHALHSMFKSTTQLLFLPRSLTRWTSTRVWKEHFDAWDVISEYANRCIWKVHQELRLGSSQTYSGIVAALITQGALPLDAIKANSMELTAGSVDTTAIPLVMTLFELARNPDVQQALRQETLAAEASIAANPQKAMSDLPLLRAALKETLRLYPVGGFLERILNSDLVLQNYHVPAGCSQDLSAICLSAGWRGKGVSSIWPSALGCASAWGGAWQRWRCCSCFTIC

Target ORF information:

RefSeq Version	XM_008765541.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765541.2

ORF Insert Sequence:

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGTGTT CCCAAGACCT GAGCGCTATA TGCCTCAGCG CTGGCTGGAG AGGAAAAGGA 
GTTTCCAGCA TCTGGCCTTC GGCTTTGGGG TGCGCCAGTG CCTGGGGCGG CGCCTGGCAG 
AGGTGGAGAT GCTGCTCCTG CTTCACCATA TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp11b2 ( XM_008765542.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763764.1 Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X3, mRNA.

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CloneID	ORa42883
Clone ID Related Accession (Same CDS sequence)	XM_008765542.2
Accession Version	XM_008765542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 11B2, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGTGTT CCCAAGACCT GAGCGCTATA TGCCTCAGCG CTGGCTGGAG AGGAAAAGGA 
GTTTCCAGCA TCTGGCCTTC GGCTTTGGGG TGCGCCAGTG CCTGGGGCGG CGCCTGGCAG 
AGGTGGAGAT GCTGCTCCTG CTTCACCATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763764.1
CDS	33..1385
Translation	MAMALRVTADVWLARPWQCLHRTRALGTTATLAPKTLKPFEAIPQYSRNKWLKMIQILREQGQENLHLEMHQAFQELGPIFRHSAGGAQIVSVMLPEDAEKLHQVESILPRRMHLEPWVAHRELRGLRRGVFLLNGADWRFNRLKLNPNVLSPKAVQNFVPMVDEVARDFLEALKKKVRQNARGSLTMDVQQSLFNYTIEASNFALFGERLGLLGHDLNPGSLKFIHALHSMFKSTTQLLFLPRSLTRWTSTRVWKEHFDAWDVISEYANRCIWKVHQELRLGSSQTYSGIVAALITQGALPLDAIKANSMELTAGSVDTTAIPLVMTLFELARNPDVQQALRQETLAAEASIAANPQKAMSDLPLLRAALKETLRLYPVGGFLERILNSDLVLQNYHVPAGCSQDLSAICLSAGWRGKGVSSIWPSALGCASAWGGAWQRWRCCSCFTM

Target ORF information:

RefSeq Version	XM_008765542.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765542.2

ORF Insert Sequence:

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAGCAACT TTGCACTTTT TGGAGAGAGG CTGGGCCTCC TTGGTCATGA CCTGAACCCT 
GGTAGCCTGA AGTTCATCCA TGCCCTACAT TCAATGTTCA AGTCCACCAC ACAGCTCCTG 
TTCTTACCCA GAAGCTTGAC TCGCTGGACA AGCACCCGGG TGTGGAAAGA ACATTTTGAT 
GCCTGGGATG TCATCTCTGA GTATGCCAAC AGATGTATCT GGAAGGTGCA CCAGGAACTC 
AGACTCGGCA GCTCTCAGAC CTACAGTGGC ATTGTGGCAG CACTAATAAC TCAGGGAGCT 
TTACCTCTGG ACGCCATCAA AGCCAACTCT ATGGAGCTCA CTGCTGGGAG CGTTGACACG 
ACAGCAATCC CCTTGGTAAT GACCCTTTTT GAGCTGGCTC GGAACCCAGA TGTTCAGCAG 
GCCCTGCGGC AGGAGACCCT GGCAGCTGAG GCCAGCATCG CTGCTAATCC CCAGAAGGCC 
ATGTCAGACC TGCCCTTGCT GCGGGCTGCC CTTAAAGAGA CCTTGAGACT CTACCCTGTT 
GGTGGCTTTT TGGAGAGAAT CCTAAACTCA GACCTGGTGC TTCAGAACTA TCATGTCCCT 
GCTGGGTGTT CCCAAGACCT GAGCGCTATA TGCCTCAGCG CTGGCTGGAG AGGAAAAGGA 
GTTTCCAGCA TCTGGCCTTC GGCTTTGGGG TGCGCCAGTG CCTGGGGCGG CGCCTGGCAG 
AGGTGGAGAT GCTGCTCCTG CTTCACCATG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyp11b2 ( XM_008765543.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763765.1 Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa42884
Clone ID Related Accession (Same CDS sequence)	XM_008765543.2
Accession Version	XM_008765543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 11B2, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAACAGAT GTATCTGGAA GGTGCACCAG GAACTCAGAC TCGGCAGCTC TCAGACCTAC 
AGTGGCATTG TGGCAGCACT AATAACTCAG GGAGCTTTAC CTCTGGACGC CATCAAAGCC 
AACTCTATGG AGCTCACTGC TGGGAGCGTT GACACGACAG CAATCCCCTT GGTAATGACC 
CTTTTTGAGC TGGCTCGGAA CCCAGATGTT CAGCAGGCCC TGCGGCAGGA GACCCTGGCA 
GCTGAGGCCA GCATCGCTGC TAATCCCCAG AAGGCCATGT CAGACCTGCC CTTGCTGCGG 
GCTGCCCTTA AAGAGACCTT GAGACTCTAC CCTGTTGGTG GCTTTTTGGA GAGAATCCTA 
AACTCAGACC TGGTGCTTCA GAACTATCAT GTCCCTGCTG GGACGTTGGT CCTACTTTAT 
CTGTACTCCA TGGGCCGAAA CCCTGCAGTG TTCCCAAGAC CTGAGCGCTA TATGCCTCAG 
CGCTGGCTGG AGAGGAAAAG GAGTTTCCAG CATCTGGCCT TCGGCTTTGG GGTGCGCCAG 
TGCCTGGGGC GGCGCCTGGC AGAGGTGGAG ATGCTGCTCC TGCTTCACCA TATGCTGAAA 
ACCTTCCAGG TGGAGACACT GAGACAAGAG GATGTGCAGA TGGCCTATCG CTTTGTTTTG 
ATGCCCAGCT CTAGTCCTGT CCTCACTTTC CGGCCCGTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763765.1
CDS	33..1337
Translation	MAMALRVTADVWLARPWQCLHRTRALGTTATLAPKTLKPFEAIPQYSRNKWLKMIQILREQGQENLHLEMHQAFQELGPIFRHSAGGAQIVSVMLPEDAEKLHQVESILPRRMHLEPWVAHRELRGLRRGVFLLNGADWRFNRLKLNPNVLSPKAVQNFVPMVDEVARDFLEALKKKVRQNARGSLTMDVQQSLFNYTIEANRCIWKVHQELRLGSSQTYSGIVAALITQGALPLDAIKANSMELTAGSVDTTAIPLVMTLFELARNPDVQQALRQETLAAEASIAANPQKAMSDLPLLRAALKETLRLYPVGGFLERILNSDLVLQNYHVPAGTLVLLYLYSMGRNPAVFPRPERYMPQRWLERKRSFQHLAFGFGVRQCLGRRLAEVEMLLLLHHMLKTFQVETLRQEDVQMAYRFVLMPSSSPVLTFRPVS

Target ORF information:

RefSeq Version	XM_008765543.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450, family 11, subfamily b, polypeptide 2 (Cyp11b2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765543.2

ORF Insert Sequence:

ATGGCAATGG CTCTCAGGGT GACAGCAGAT GTGTGGCTGG CAAGACCCTG GCAGTGCCTG 
CACAGGACGA GGGCACTGGG CACTACGGCA ACACTGGCCC CCAAGACACT GAAGCCCTTT 
GAAGCCATAC CACAATACTC CAGGAACAAG TGGCTGAAGA TGATACAGAT CCTGAGGGAG 
CAGGGCCAAG AGAACCTACA CCTGGAGATG CACCAGGCCT TCCAGGAGCT GGGGCCCATT 
TTCAGGCACA GTGCAGGGGG AGCACAGATT GTGTCTGTGA TGCTGCCTGA GGACGCTGAG 
AAGCTGCACC AGGTGGAGAG TATCCTCCCG CGTCGGATGC ACCTGGAGCC GTGGGTGGCC 
CACAGGGAAC TCCGTGGCCT GAGACGTGGT GTGTTCTTGC TAAATGGGGC TGACTGGCGC 
TTCAACCGAC TGAAACTGAA CCCAAACGTG CTGTCACCAA AAGCTGTTCA AAATTTTGTC 
CCCATGGTGG ACGAGGTAGC AAGGGACTTC TTGGAGGCCC TGAAAAAGAA GGTGCGTCAG 
AATGCTCGAG GGAGCCTTAC CATGGATGTC CAGCAAAGTC TCTTCAACTA CACTATAGAA 
GCCAACAGAT GTATCTGGAA GGTGCACCAG GAACTCAGAC TCGGCAGCTC TCAGACCTAC 
AGTGGCATTG TGGCAGCACT AATAACTCAG GGAGCTTTAC CTCTGGACGC CATCAAAGCC 
AACTCTATGG AGCTCACTGC TGGGAGCGTT GACACGACAG CAATCCCCTT GGTAATGACC 
CTTTTTGAGC TGGCTCGGAA CCCAGATGTT CAGCAGGCCC TGCGGCAGGA GACCCTGGCA 
GCTGAGGCCA GCATCGCTGC TAATCCCCAG AAGGCCATGT CAGACCTGCC CTTGCTGCGG 
GCTGCCCTTA AAGAGACCTT GAGACTCTAC CCTGTTGGTG GCTTTTTGGA GAGAATCCTA 
AACTCAGACC TGGTGCTTCA GAACTATCAT GTCCCTGCTG GGACGTTGGT CCTACTTTAT 
CTGTACTCCA TGGGCCGAAA CCCTGCAGTG TTCCCAAGAC CTGAGCGCTA TATGCCTCAG 
CGCTGGCTGG AGAGGAAAAG GAGTTTCCAG CATCTGGCCT TCGGCTTTGG GGTGCGCCAG 
TGCCTGGGGC GGCGCCTGGC AGAGGTGGAG ATGCTGCTCC TGCTTCACCA TATGCTGAAA 
ACCTTCCAGG TGGAGACACT GAGACAAGAG GATGTGCAGA TGGCCTATCG CTTTGTTTTG 
ATGCCCAGCT CTAGTCCTGT CCTCACTTTC CGGCCCGTCA GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Aldosterone Synthase CYP11B2 Gene Promoter Polymorphism in a Turkish Population With Chronic Kidney Disease.
ian journal of kidney diseases9(3)209-14(2015 May)
Yilmaz M,Sari I,Bagci B,Gumus E,Ozdemir O

[The influence of suoquan capsule on the mRNA expression of CYP11B2 in deficiency of the kidney and diuresis rats].
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials34(1)80-3(2011 Jan)
Li SW,Hu LJ,Wu QH,Huang P,Cao HY

Sodium depletion increases sympathetic neurite outgrowth and expression of a novel TMEM35 gene-derived protein (TUF1) in the rat adrenal zona glomerulosa.
Endocrinology151(10)4852-60(2010 Oct)
Tran PV,Georgieff MK,Engeland WC

In vivo regulation of gene expression of enzymes controlling aldosterone synthesis in rat adrenal.
The Journal of steroid biochemistry and molecular biology43(8)837-46(1992 Dec)
LeHoux JG,Tremblay A

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Cyp11b2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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