Chrm3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chrm3
Entrez Gene ID	24260
Full Name	cholinergic receptor, muscarinic 3
General protein information	Preferred Names cholinergic receptor, muscarinic 3 Names muscarinic acetylcholine receptor M3 cholinergic receptor, muscarinic 3, cardiac
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 17 Map Location: 17q12.1
Summary	G-protein coupled receptor, muscarinic receeptor subtype that mediates cholinergic-induced contraction in most smooth muscle [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017600452.1	XP_017455941.1	muscarinic acetylcholine receptor M3 isoform X1
XM_017600449.1	XP_017455938.1	muscarinic acetylcholine receptor M3 isoform X1
XM_017600451.1	XP_017455940.1	muscarinic acetylcholine receptor M3 isoform X1
XM_017600450.1	XP_017455939.1	muscarinic acetylcholine receptor M3 isoform X1
NM_012527.2	NP_036659.2	muscarinic acetylcholine receptor M3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHRM3	NP_000731.1
	CHRM3	XP_001096287.1
Bos taurus (cattle)	CHRM3	NP_776695.1
Mus musculus (house mouse)	Chrm3	NP_150372.1
Gallus gallus (chicken)	CHRM3	NP_990730.1
Rattus norvegicus (Norway rat)	Chrm3	NP_036659.2
Danio rerio (zebrafish)	chrm3a	XP_700381.4
Xenopus tropicalis (tropical clawed frog)	LOC100493547	XP_002935876.1
Xenopus tropicalis (tropical clawed frog)	LOC101730815	XP_004920895.1
Canis lupus familiaris (dog)	CHRM3	XP_546069.4
Pan troglodytes (chimpanzee)	CHRM3	NP_001129093.1

Gene Ontology
Bibliography

Related articles in PubMed

M3 cholinoreceptors alter electrical activity of rat left atrium via suppression of L-type Ca
Filatova TS, Naumenko N, Galenko-Yaroshevsky PA, Abramochkin DV
Journal of physiology and biochemistry73(2)167-174(2017 May)

M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product.
Garcia RC, Dati LM, Torres LH, da Silva MA, Udo MS, Abdalla FM, da Costa JL, Gorj?o R, Afeche SC, Yonamine M, Niswender CM, Conn PJ, Camarini R, Sandoval MR, Marcourakis T
Scientific reports517555(2015 Dec)

The expression of ?3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat.
Eastham J, Stephenson C, Korstanje K, Gillespie JI
Naunyn-Schmiedeberg's archives of pharmacology388(7)695-708(2015 Jul)

Different Myocardial Sensitivity in Newborn and Mature Rats to Selective Stimulation of M3 Cholinoreceptors.
Tapilina SV, Abramochkin DV
Bulletin of experimental biology and medicine159(1)8-10(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that, in atrial myocardium (or isolated cardiomyocytes), activation of muscarinic receptors M3 (Chrm3) leads to shortening of action potentials via suppression of L-type calcium channels; the major potassium channels are not affected, but inwardly rectifying potassium channels are suppressed.
Title: M3 cholinoreceptors alter electrical activity of rat left atrium via suppression of L-type Ca

M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product.
Title: M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product.

The expression of beta3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat.
Title: The expression of ?3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat.

The ventricular myocardium of rat pups differs from that of mature rats by a far greater sensitivity to selective stimulation of M3 receptors, suggesting an important functional role of M3 receptors in the newborn rats, which declines with age.
Title: Different Myocardial Sensitivity in Newborn and Mature Rats to Selective Stimulation of M3 Cholinoreceptors.

Activation of muscarinic receptors leads to rapid activation of several kinases that regulates contraction of longitudinal muscle of colon.
Title: Role of various kinases in muscarinic M3 receptor-mediated contraction of longitudinal muscle of rat colon.

The following Chrm3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chrm3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00019	NM_012527.2 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa00019	XM_017600451.1 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa00019	XM_017600449.1 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa00019	XM_017600452.1 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa00019	XM_017600450.1 Latest version!	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chrm3 ( NM_012527.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036659.2 Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), mRNA.

Price & Availability↑

CloneID	ORa00019
Clone ID Related Accession (Same CDS sequence)	XM_017600452.1 , XM_017600449.1 , XM_017600451.1 , XM_017600450.1 , NM_012527.2
Accession Version	NM_012527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07028312.1, AABR07028310.1, AABR07028305.1, AABR07028303.1 and AABR07072561.1. On Feb 14, 2013 this sequence version replaced NM_012527.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BF408808.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036659.2
CDS	548..2317
Translation	MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQETGNFSSNDTSSDPLGGHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFVHPTGSSRSCSSYELQQQGVKRSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSSILNSTKLPSSDNLQVSNEDLGTVDVERNAHKLQAQKSMGDGDNCQKDFTKLPIQLESAVDTGKTSDTNSSADKTTATLPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLLLCQCDKRKRRKQQYQQRQSVIFHKRVPEQAL

Target ORF information:

RefSeq Version	NM_012527.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012527.2

ORF Insert Sequence:

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrm3 ( XM_017600452.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455941.1 Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa00019
Clone ID Related Accession (Same CDS sequence)	XM_017600452.1 , XM_017600449.1 , XM_017600451.1 , XM_017600450.1 , NM_012527.2
Accession Version	XM_017600452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455941.1
CDS	615..2384
Translation	MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQETGNFSSNDTSSDPLGGHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFVHPTGSSRSCSSYELQQQGVKRSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSSILNSTKLPSSDNLQVSNEDLGTVDVERNAHKLQAQKSMGDGDNCQKDFTKLPIQLESAVDTGKTSDTNSSADKTTATLPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLLLCQCDKRKRRKQQYQQRQSVIFHKRVPEQAL

Target ORF information:

RefSeq Version	XM_017600452.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600452.1

ORF Insert Sequence:

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrm3 ( XM_017600449.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455938.1 Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00019
Clone ID Related Accession (Same CDS sequence)	XM_017600452.1 , XM_017600449.1 , XM_017600451.1 , XM_017600450.1 , NM_012527.2
Accession Version	XM_017600449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455938.1
CDS	264..2033
Translation	MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQETGNFSSNDTSSDPLGGHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFVHPTGSSRSCSSYELQQQGVKRSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSSILNSTKLPSSDNLQVSNEDLGTVDVERNAHKLQAQKSMGDGDNCQKDFTKLPIQLESAVDTGKTSDTNSSADKTTATLPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLLLCQCDKRKRRKQQYQQRQSVIFHKRVPEQAL

Target ORF information:

RefSeq Version	XM_017600449.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600449.1

ORF Insert Sequence:

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrm3 ( XM_017600451.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455940.1 Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa00019
Clone ID Related Accession (Same CDS sequence)	XM_017600452.1 , XM_017600449.1 , XM_017600451.1 , XM_017600450.1 , NM_012527.2
Accession Version	XM_017600451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455940.1
CDS	946..2715
Translation	MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQETGNFSSNDTSSDPLGGHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFVHPTGSSRSCSSYELQQQGVKRSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSSILNSTKLPSSDNLQVSNEDLGTVDVERNAHKLQAQKSMGDGDNCQKDFTKLPIQLESAVDTGKTSDTNSSADKTTATLPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLLLCQCDKRKRRKQQYQQRQSVIFHKRVPEQAL

Target ORF information:

RefSeq Version	XM_017600451.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600451.1

ORF Insert Sequence:

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chrm3 ( XM_017600450.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455939.1 Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa00019
Clone ID Related Accession (Same CDS sequence)	XM_017600452.1 , XM_017600449.1 , XM_017600451.1 , XM_017600450.1 , NM_012527.2
Accession Version	XM_017600450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	muscarinic acetylcholine receptor M3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005116.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455939.1
CDS	4391..6160
Translation	MTLHSNSTTSPLFPNISSSWVHSPSEAGLPLGTVTQLGSYNISQETGNFSSNDTSSDPLGGHTIWQVVFIAFLTGFLALVTIIGNILVIVAFKVNKQLKTVNNYFLLSLACADLIIGVISMNLFTTYIIMNRWALGNLACDLWLSIDYVASNASVMNLLVISFDRYFSITRPLTYRAKRTTKRAGVMIGLAWVISFVLWAPAILFWQYFVGKRTVPPGECFIQFLSEPTITFGTAIAAFYMPVTIMTILYWRIYKETEKRTKELAGLQASGTEAEAENFVHPTGSSRSCSSYELQQQGVKRSSRRKYGRCHFWFTTKSWKPSAEQMDQDHSSSDSWNNNDAAASLENSASSDEEDIGSETRAIYSIVLKLPGHSSILNSTKLPSSDNLQVSNEDLGTVDVERNAHKLQAQKSMGDGDNCQKDFTKLPIQLESAVDTGKTSDTNSSADKTTATLPLSFKEATLAKRFALKTRSQITKRKRMSLIKEKKAAQTLSAILLAFIITWTPYNIMVLVNTFCDSCIPKTYWNLGYWLCYINSTVNPVCYALCNKTFRTTFKTLLLCQCDKRKRRKQQYQQRQSVIFHKRVPEQAL

Target ORF information:

RefSeq Version	XM_017600450.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cholinergic receptor, muscarinic 3 (Chrm3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600450.1

ORF Insert Sequence:

ATGACCTTGC ACAGTAACAG TACAACCTCG CCTTTGTTTC CCAACATCAG CTCTTCCTGG 
GTGCACAGTC CCTCGGAGGC AGGGCTGCCC TTGGGGACAG TCACTCAGTT GGGCAGCTAC 
AACATTTCAC AAGAAACTGG GAATTTCTCC TCAAACGACA CCTCCAGCGA CCCTCTCGGG 
GGTCACACCA TCTGGCAAGT GGTCTTCATT GCCTTCTTAA CCGGCTTCCT GGCATTGGTG 
ACCATCATTG GCAACATCCT TGTCATTGTG GCCTTCAAGG TCAACAAACA GCTGAAGACA 
GTCAACAACT ACTTCCTCTT AAGCCTGGCC TGTGCAGACC TGATCATCGG GGTCATTTCC 
ATGAACCTGT TCACTACCTA CATCATTATG AACCGTTGGG CACTGGGGAA CTTAGCCTGC 
GACCTCTGGC TTTCCATTGA CTATGTGGCC AGCAATGCCT CTGTCATGAA TCTGCTGGTC 
ATCAGCTTTG ACAGGTACTT TTCCATCACT AGACCACTCA CCTACCGAGC CAAAAGAACA 
ACAAAACGAG CTGGTGTGAT GATTGGTCTG GCTTGGGTCA TCTCCTTTGT CCTATGGGCT 
CCTGCCATCT TGTTCTGGCA ATACTTTGTA GGGAAGAGAA CTGTGCCCCC AGGAGAATGT 
TTCATTCAGT TTCTGAGTGA GCCCACCATC ACCTTCGGCA CGGCGATCGC TGCCTTTTAC 
ATGCCTGTCA CCATCATGAC TATTTTATAC TGGAGGATCT ATAAGGAAAC TGAGAAGCGT 
ACCAAAGAGC TGGCTGGCCT ACAGGCCTCT GGGACAGAAG CGGAGGCAGA AAACTTTGTC 
CACCCCACAG GCAGTTCTCG AAGCTGTAGC AGCTATGAAC TGCAACAGCA AGGCGTGAAA 
CGATCATCCA GGAGGAAGTA CGGTCGCTGT CACTTCTGGT TCACCACCAA GAGCTGGAAG 
CCCAGTGCCG AGCAGATGGA CCAAGACCAC AGCAGCAGCG ACAGTTGGAA CAACAACGAT 
GCTGCTGCCT CCCTGGAAAA CTCTGCTTCC TCCGATGAAG AGGACATTGG CTCAGAGACC 
AGGGCCATCT ATTCCATTGT CCTCAAGCTT CCAGGCCATA GCTCCATCCT CAACTCTACC 
AAGCTACCGT CCTCAGATAA CCTGCAGGTG TCCAACGAGG ACCTGGGGAC TGTGGATGTG 
GAGAGAAATG CTCACAAGCT TCAGGCCCAG AAGAGCATGG GTGATGGTGA CAACTGTCAG 
AAGGATTTCA CCAAGCTTCC CATCCAGTTA GAGTCTGCCG TGGACACAGG CAAGACCTCT 
GACACCAACT CCTCGGCAGA CAAGACCACG GCTACTCTAC CTCTGTCCTT CAAGGAGGCC 
ACGCTGGCTA AGAGGTTTGC TCTCAAGACC AGAAGTCAGA TCACCAAGCG GAAGAGGATG 
TCGCTCATCA AGGAGAAGAA GGCCGCCCAG ACGCTCAGTG CCATCTTGCT AGCCTTCATC 
ATCACGTGGA CCCCCTACAA CATCATGGTC CTGGTGAACA CCTTCTGTGA CAGCTGCATA 
CCCAAAACCT ATTGGAATCT GGGCTACTGG CTGTGCTATA TCAACAGCAC CGTGAACCCT 
GTGTGCTATG CCCTGTGCAA CAAAACATTC AGAACCACCT TCAAGACGCT CCTCTTGTGC 
CAGTGTGACA AAAGGAAGAG GCGCAAACAG CAGTACCAGC AGAGACAGTC GGTCATTTTT 
CACAAGCGAG TGCCGGAACA GGCCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

M3 cholinoreceptors alter electrical activity of rat left atrium via suppression of L-type Ca
Journal of physiology and biochemistry73(2)167-174(2017 May)
Filatova TS,Naumenko N,Galenko-Yaroshevsky PA,Abramochkin DV

M1 and M3 muscarinic receptors may play a role in the neurotoxicity of anhydroecgonine methyl ester, a cocaine pyrolysis product.
Scientific reports517555(2015 Dec)
Garcia RC,Dati LM,Torres LH,da Silva MA,Udo MS,Abdalla FM,da Costa JL,Gorj?o R,Afeche SC,Yonamine M,Niswender CM,Conn PJ,Camarini R,Sandoval MR,Marcourakis T

The expression of ?3-adrenoceptor and muscarinic type 3 receptor immuno-reactivity in the major pelvic ganglion of the rat.
Naunyn-Schmiedeberg's archives of pharmacology388(7)695-708(2015 Jul)
Eastham J,Stephenson C,Korstanje K,Gillespie JI

Different Myocardial Sensitivity in Newborn and Mature Rats to Selective Stimulation of M3 Cholinoreceptors.
Bulletin of experimental biology and medicine159(1)8-10(2015 May)
Tapilina SV,Abramochkin DV

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Chrm3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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