Cftr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cftr
Entrez Gene ID	24255
Full Name	cystic fibrosis transmembrane conductance regulator
Synonyms	RGD1561193
General protein information	Preferred Names cystic fibrosis transmembrane conductance regulator Names cystic fibrosis transmembrane conductance regulator ATP-binding cassette sub-family C member 7 ATP-binding cassette transporter sub-family C member 7 cAMP-dependent chloride channel channel conductance-controlling ATPase cystic fibrosis transmembrane conductance regulator homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q21
Summary	cAMP-activated chloride channel and ATP binding cassette (ABC) transporter involved in intestinal anion secretion [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006236097.3	XP_006236159.1	cystic fibrosis transmembrane conductance regulator isoform X1
NM_031506.1	NP_113694.1	cystic fibrosis transmembrane conductance regulator

Pathways from BioSystems

Homologies

	CFTR	NP_001028110.1
Pan troglodytes (chimpanzee)	CFTR	NP_001073386.1
Rattus norvegicus (Norway rat)	Cftr	NP_113694.1
Canis lupus familiaris (dog)	CFTR	NP_001007144.1
Mus musculus (house mouse)	Cftr	NP_066388.1
Xenopus tropicalis (tropical clawed frog)	cftr	XP_002935002.2
Homo sapiens (human)	CFTR	NP_000483.3
Bos taurus (cattle)	CFTR	NP_776443.1
Gallus gallus (chicken)	CFTR	NP_001099136.1
Danio rerio (zebrafish)	cftr	NP_001038348.1

Gene Ontology
Bibliography

Related articles in PubMed

Reduced bone length, growth plate thickness, bone content, and IGF-I as a model for poor growth in the CFTR-deficient rat.
Stalvey MS, Havasi V, Tuggle KL, Wang D, Birket S, Rowe SM, Sorscher EJ
PloS one12(11)e0188497(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings appear to be intrinsic to the CFTR deficient state and independent of significant clinical confounders, providing substantive evidence for the importance of CFTR on maintinaing normal bone growth
Title: Reduced bone length, growth plate thickness, bone content, and IGF-I as a model for poor growth in the CFTR-deficient rat.

Increased levels of CFTR, adenylate cyclase and cAMP in cervix of rats treated with sex hormones and genistein could affect the cervical secretory function, influencing female reproductive processes.
Title: Combinatorial effects of genistein and sex-steroids on the level of cystic fibrosis transmembrane regulator (CFTR), adenylate cyclase (AC) and cAMP in the cervix of ovariectomised rats.

increased abundance of CFTR, AC, and cAMP under estrogen influence could account for the increased fluid accumulation within the cervical lumen.
Title: Estrogen and progesterone differentially regulate the levels of cystic fibrosis transmembrane regulator (CFTR), adenylate cyclase (AC), and cyclic adenosine mono-phosphate (cAMP) in the rat cervix.

CFTR Cl- channel is a molecular target of natural compounds EGCG and ECG.
Title: Bioactivity-guided fractionation of an antidiarrheal Chinese herb Rhodiola kirilowii (Regel) Maxim reveals (-)-epicatechin-3-gallate and (-)-epigallocatechin-3-gallate as inhibitors of cystic fibrosis transmembrane conductance regulator.

CFTR is involved in water homeostasis of the seminiferous tubular secretion.
Title: Aquaporin-9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator.

The following Cftr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cftr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13344	NM_031506.1 Latest version!	Rattus norvegicus CF transmembrane conductance regulator (Cftr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa27035	XM_006236097.3 Latest version!	Rattus norvegicus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cftr ( NM_031506.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113694.1 Rattus norvegicus CF transmembrane conductance regulator (Cftr), mRNA.

Price & Availability↑

CloneID	ORa13344
Clone ID Related Accession (Same CDS sequence)	NM_031506.1
Accession Version	NM_031506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Rattus norvegicus(Norway rat)
Product	cystic fibrosis transmembrane conductance regulator
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DP000027.1. On or before Apr 14, 2006 this sequence version replaced XM_342645.2, XM_347229.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689604 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAAGT CGCCTCTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGAAAAGG GTACAGACAC CACTTGGAGC TGTCAGACAT ATACCAAGCC 
CCTTCTTCTG ATTCAGCTGA CCACCTGTCT GAAAAGCTAG AAAGAGAATG GGACAGGGAA 
CAAGCTTCCA AAAAGAAGCC CCAGCTTATC CACGCCCTCC GGCGATGCTT TGTCTGGAGA 
TTCGTCTTCT ACGGAGTCTT GTTATACCTC GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGACAACA CGGAGGAGCG TTCCATTGCC 
ATTTACCTAG GCATAGGCTT ATGCCTCCTC TTCATTGTCA GGACACTGCT TCTGCACCCA 
GCTATTTTTG GCCTTCATCA CATTGGAATG CAGATGAGAA TAGCCATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGT GTTCTTGATA AAATAAGTAT TGGACAACTT 
ATTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGGTTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAGTCTTGG TTATTTTTCA AGCTATCTTA 
GGGAAGATGA TGGTGAAGTA CAGAGATAAA AGAGCTGCAA AGATCAATGA AAGACTTGTG 
ATCACATCAG AAGTTATTGA CAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAGA TAATTGAAAG CTTGAGAGAG GAGGAACTGA AAATGACCCG GAGGTCAGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTTTTTGT AGTCTTTTTA 
TCTGTGCTTC CCTACACAGT CATCAATGGA ATCGTCCTCC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTGCG TATGTCAGTC ACACGGCAAT TCCCCACTGC TGTACAGATA 
TGGTATGATT CTTTGGGAAT GATAAGAAAA ATACAGGATT TCCTACAGAC TCAAGAATAT 
AAAGTGCTGG AGTATAACTT AATGTTCACA GGCCTAGTCA TGGAGAATGT AACAGCATTT 
TGGGAGGAGG GATTTCAGGA ATTACTGGAG AAAGTACAAC TAAACAATGA TGACAGAAAA 
ACTTCCAATG GTGAGAACCA CCTCAGTTTT AGTCATCTCT GTCTAGTGGG AAATCCTGTG 
TTGAAAAACA TCAATTTGAA TATAAAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGAGCAGGAA AGACATCACT CCTGATGCTG ATACTGGGAG AACTGGAAGC GTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TCCTCTCAAA TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GGAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTCAGTGGA GGTCAACGTG CAAGAATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTG TTAGATTCCC CCTTCGGATA TCTAGATGTT 
TTAACTGAAG AACAAATATT TGAAAGTTGT GTCTGTAAAT TGATGGCCAG CAAAACTAGG 
ATTCTGGTCA CATCTAAAAT GGAGCAGTTA AAGAAAGCTG ACAAGATACT CATTTTGCAT 
GAGGGCAGCA GCTATTTTTA TGGGACGTTT TCTGAACTAC AAAGTCTACG TCCAGACTTC 
AGTTCCAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGGAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTGGACGATG CCTCTACCAC CTGGAACAAA 
GCCAAACAGT CATTTAGACA GACTGGAGAG TTTGGAGAAA AAAGGAAGAA CTCTATTCTA 
AGTTCATTCA GCTCTGTGAA GAAAATTTCC ATTGTGCAAA AGACTCCATT GTCCATAGAA 
GGAGAATCTG ATGACCTCCA AGAAAGGAGA CTGTCCCTGG TTCCAGACTC TGAACATGGA 
GAGGCCGCTC TGCCTCGCAG CAACATGATC ACCGCCGGGC CCACGTTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT TATGACATTC ACACCCAGCT CAGTCTCCAG CAGTCTCCAG 
AGGACCAGAG CTTCTATTCG AAAAATATCC TTAGCCCCTC GGATAAGCTT AAAGGAAGAG 
GATATATATT CAAGGCGATT ATCGCAAGAT AGCACACTGA ACATCACCGA AGAAATTAAT 
GAAGAAGATT TAAAGGAGTG TTTTTTTGAT GATATGGTAA AGATCCCCAC GGTGACAACA 
TGGAACACAT ACCTTCGATA TTTCACGCTC CACAGAGGCT TATTTGCAGT GCTGATTTGG 
TGCGTGCTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTGTG GTTGCTTAAA 
AACAACCCCG TTAATGGTGG AAACAATGGC ACTAAAATTG CCAATACCTC CTATGTTGTG 
GTCATCACCA GTTCCAGCTT CTATTACATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCTC TGAGCCTCTT CAGAGGTTTG CCCCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACATTCT ATTCTTCATG CCCCTATGTC AACCTTCAAC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATATT TGACTTCATC CAGTTGCTGT TCATTGTGGT CGGAGCAATA 
ATAGTTGTCT CGGCTTTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGCAGTC 
TTCATTTTAC TGAGGGCATA CTTCCTTCAT ACCTCCCAAC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACGCACCTT GTTACAAGCT TAAAAGGACT GTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACCCTATTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAGATGAG AATAGACATG 
ATTTTCGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACGA CTGGTATTAT TCTAACTTTA GCTATGAATA TTATGAGTAC TTTGCAATGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTCTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATA TGTACCAAGA TAATGAAAGA ACTACATAGT 
GAAGACTCAC CTAATGCTTT AGTCATTAAG AATGAACATG TGAAGAAATG TGATACCTGG 
CCCTCTGGAG GTGAAATGGT TGTCAAAGAC CTTACTGTGA AATATGTGGA TGATGGGAAT 
GCCATATTAG AGAACATTTC TTTTTCAATA AGTCCTGGCC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGCT CAGGAAAAAG TACTTTGCTG TCAGCCTTTT TACGAATGTT GAACATTAAA 
GGCGAAATAC AGATCGATGG TGTCTCCTGG AACTCAATGA CCTTACAAGA ATGGAGAAAA 
GCTTTTGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACTTG 
GATCCCAATG GAAAATGGAG AGATGAAGAA ATATGGAAAG TTGCAGATCA GGTTGGCCTC 
AAGTCTGTGA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGTCACAA GCAGTTGATG TGCTTGGCCC GATCTGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCTAGTGCC AATCTAGACC CCATAACATA CCAAGTGATT 
CGACGAGTTC TAAGACAAGC CTTCGCTGGT TGCACAGTAG TCCTCTGTGA ACACAGGATA 
GAAGCAATGT TGGACTGTCA GCGATTTTTG GTCATAGAGC AGGGCAATGT CTGGCAGTAT 
GAATCCCTCC AGGCACTTCT GAGCGAGAAG AGTGTCTTCC AGCGAGCCCT TAGCTCCTCA 
GAAAAGATGA AGCTCTTCCA CGGCCGGCAC TCCAGCAAGC AGAAGCCTCG GACACAGATC 
ACTGCTGTGA AAGAGGAGAC GGAGGAAGAA GTGCAAGAGA CCCGGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113694.1
CDS	138..4568
Translation	MQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSSDSADHLSEKLEREWDREQASKKKPQLIHALRRCFVWRFVFYGVLLYLGEVTKAVQPVLLGRIIASYDPDNTEERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLISLLSNNLNKFDEGLALAHFIWIAPLQVVLLMGLLWDLLQFSAFCGLGLLIVLVIFQAILGKMMVKYRDKRAAKINERLVITSEVIDNIYSVKAYCWESAMEKIIESLREEELKMTRRSAYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSLGMIRKIQDFLQTQEYKVLEYNLMFTGLVMENVTAFWEEGFQELLEKVQLNNDDRKTSNGENHLSFSHLCLVGNPVLKNINLNIKKGEMLAITGSTGAGKTSLLMLILGELEASEGIIKHSGRVSFSSQISWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQEDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEEQIFESCVCKLMASKTRILVTSKMEQLKKADKILILHEGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDASTTWNKAKQSFRQTGEFGEKRKNSILSSFSSVKKISIVQKTPLSIEGESDDLQERRLSLVPDSEHGEAALPRSNMITAGPTFPGRRRQSVLDLMTFTPSSVSSSLQRTRASIRKISLAPRISLKEEDIYSRRLSQDSTLNITEEINEEDLKECFFDDMVKIPTVTTWNTYLRYFTLHRGLFAVLIWCVLVFLVEVAASLFVLWLLKNNPVNGGNNGTKIANTSYVVVITSSSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTFNKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLLFIVVGAIIVVSALQPYIFLATVPGLAVFILLRAYFLHTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTTGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRVFKFIDIQTEESICTKIMKELHSEDSPNALVIKNEHVKKCDTWPSGGEMVVKDLTVKYVDDGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGEIQIDGVSWNSMTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWRDEEIWKVADQVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSANLDPITYQVIRRVLRQAFAGCTVVLCEHRIEAMLDCQRFLVIEQGNVWQYESLQALLSEKSVFQRALSSSEKMKLFHGRHSSKQKPRTQITAVKEETEEEVQETRL

Target ORF information:

RefSeq Version	NM_031506.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus CF transmembrane conductance regulator (Cftr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031506.1

ORF Insert Sequence:

ATGCAGAAGT CGCCTCTGGA GAAAGCCAGC TTTATCTCCA AACTCTTCTT CAGCTGGACC 
ACACCAATTT TGAGAAAAGG GTACAGACAC CACTTGGAGC TGTCAGACAT ATACCAAGCC 
CCTTCTTCTG ATTCAGCTGA CCACCTGTCT GAAAAGCTAG AAAGAGAATG GGACAGGGAA 
CAAGCTTCCA AAAAGAAGCC CCAGCTTATC CACGCCCTCC GGCGATGCTT TGTCTGGAGA 
TTCGTCTTCT ACGGAGTCTT GTTATACCTC GGGGAAGTCA CCAAGGCTGT CCAGCCTGTC 
TTGCTAGGAA GAATCATAGC ATCCTATGAT CCAGACAACA CGGAGGAGCG TTCCATTGCC 
ATTTACCTAG GCATAGGCTT ATGCCTCCTC TTCATTGTCA GGACACTGCT TCTGCACCCA 
GCTATTTTTG GCCTTCATCA CATTGGAATG CAGATGAGAA TAGCCATGTT TAGCTTGATT 
TATAAGAAGA CTTTAAAGTT GTCAAGCCGT GTTCTTGATA AAATAAGTAT TGGACAACTT 
ATTAGTCTTC TTTCCAACAA CCTGAACAAA TTTGATGAAG GACTTGCCTT GGCACATTTT 
ATATGGATTG CTCCTTTACA AGTGGTTCTT CTGATGGGGC TTCTCTGGGA CTTGTTACAG 
TTCTCAGCCT TCTGTGGCCT TGGTTTACTG ATAGTCTTGG TTATTTTTCA AGCTATCTTA 
GGGAAGATGA TGGTGAAGTA CAGAGATAAA AGAGCTGCAA AGATCAATGA AAGACTTGTG 
ATCACATCAG AAGTTATTGA CAATATCTAT TCTGTTAAGG CATATTGTTG GGAATCAGCG 
ATGGAGAAGA TAATTGAAAG CTTGAGAGAG GAGGAACTGA AAATGACCCG GAGGTCAGCC 
TATATGAGGT TCTTCACTAG CTCTGCCTTC TTCTTTTCAG GGTTTTTTGT AGTCTTTTTA 
TCTGTGCTTC CCTACACAGT CATCAATGGA ATCGTCCTCC GAAAAATATT CACAACCATT 
TCATTCTGCA TTGTCCTGCG TATGTCAGTC ACACGGCAAT TCCCCACTGC TGTACAGATA 
TGGTATGATT CTTTGGGAAT GATAAGAAAA ATACAGGATT TCCTACAGAC TCAAGAATAT 
AAAGTGCTGG AGTATAACTT AATGTTCACA GGCCTAGTCA TGGAGAATGT AACAGCATTT 
TGGGAGGAGG GATTTCAGGA ATTACTGGAG AAAGTACAAC TAAACAATGA TGACAGAAAA 
ACTTCCAATG GTGAGAACCA CCTCAGTTTT AGTCATCTCT GTCTAGTGGG AAATCCTGTG 
TTGAAAAACA TCAATTTGAA TATAAAGAAA GGAGAGATGT TGGCTATTAC TGGATCTACT 
GGAGCAGGAA AGACATCACT CCTGATGCTG ATACTGGGAG AACTGGAAGC GTCAGAGGGA 
ATTATTAAGC ACAGTGGAAG AGTTTCATTC TCCTCTCAAA TTTCTTGGAT TATGCCGGGT 
ACTATCAAAG AAAATATCAT CTTTGGTGTT TCCTATGATG AGTACAGATA TAAGAGTGTT 
GTCAAAGCTT GCCAACTACA GGAGGACATC ACCAAGTTTG CAGAACAAGA CAACACAGTT 
CTTGGAGAAG GTGGAGTCAC ACTCAGTGGA GGTCAACGTG CAAGAATTTC TTTAGCAAGA 
GCAGTATATA AAGATGCTGA TTTGTACCTG TTAGATTCCC CCTTCGGATA TCTAGATGTT 
TTAACTGAAG AACAAATATT TGAAAGTTGT GTCTGTAAAT TGATGGCCAG CAAAACTAGG 
ATTCTGGTCA CATCTAAAAT GGAGCAGTTA AAGAAAGCTG ACAAGATACT CATTTTGCAT 
GAGGGCAGCA GCTATTTTTA TGGGACGTTT TCTGAACTAC AAAGTCTACG TCCAGACTTC 
AGTTCCAAAC TCATGGGGTA TGATACTTTT GACCAGTTTA CTGAGGAAAG AAGGAGTTCA 
ATTCTAACTG AGACCTTACG CAGGTTCTCA GTGGACGATG CCTCTACCAC CTGGAACAAA 
GCCAAACAGT CATTTAGACA GACTGGAGAG TTTGGAGAAA AAAGGAAGAA CTCTATTCTA 
AGTTCATTCA GCTCTGTGAA GAAAATTTCC ATTGTGCAAA AGACTCCATT GTCCATAGAA 
GGAGAATCTG ATGACCTCCA AGAAAGGAGA CTGTCCCTGG TTCCAGACTC TGAACATGGA 
GAGGCCGCTC TGCCTCGCAG CAACATGATC ACCGCCGGGC CCACGTTTCC AGGCAGAAGA 
AGACAGTCTG TTTTGGATCT TATGACATTC ACACCCAGCT CAGTCTCCAG CAGTCTCCAG 
AGGACCAGAG CTTCTATTCG AAAAATATCC TTAGCCCCTC GGATAAGCTT AAAGGAAGAG 
GATATATATT CAAGGCGATT ATCGCAAGAT AGCACACTGA ACATCACCGA AGAAATTAAT 
GAAGAAGATT TAAAGGAGTG TTTTTTTGAT GATATGGTAA AGATCCCCAC GGTGACAACA 
TGGAACACAT ACCTTCGATA TTTCACGCTC CACAGAGGCT TATTTGCAGT GCTGATTTGG 
TGCGTGCTGG TTTTTCTGGT TGAGGTGGCT GCTTCTTTAT TTGTGTTGTG GTTGCTTAAA 
AACAACCCCG TTAATGGTGG AAACAATGGC ACTAAAATTG CCAATACCTC CTATGTTGTG 
GTCATCACCA GTTCCAGCTT CTATTACATT TTTTACATTT ACGTGGGAGT GGCTGACACT 
TTGCTTGCTC TGAGCCTCTT CAGAGGTTTG CCCCTGGTGC ATACGTTAAT CACAGCATCA 
AAAATTTTGC ACAGGAAAAT GTTACATTCT ATTCTTCATG CCCCTATGTC AACCTTCAAC 
AAGCTGAAAG CAGGTGGGAT TCTTAACAGA TTCTCCAAAG ATATAGCAAT TTTGGATGAC 
TTTCTGCCTC TTACCATATT TGACTTCATC CAGTTGCTGT TCATTGTGGT CGGAGCAATA 
ATAGTTGTCT CGGCTTTACA ACCCTACATC TTCCTAGCAA CGGTGCCAGG GCTAGCAGTC 
TTCATTTTAC TGAGGGCATA CTTCCTTCAT ACCTCCCAAC AGCTCAAACA ACTGGAATCT 
GAAGGCAGGA GTCCAATTTT CACGCACCTT GTTACAAGCT TAAAAGGACT GTGGACACTT 
CGAGCCTTCC GACGCCAGAC TTACTTTGAA ACCCTATTCC ACAAAGCTCT GAATTTGCAC 
ACTGCCAACT GGTTTATGTA TCTGGCAACC TTGCGCTGGT TCCAGATGAG AATAGACATG 
ATTTTCGTCC TCTTCTTCAT TGTTGTTACC TTCATCTCCA TTTTAACAAC AGGTGAAGGA 
GAAGGAACGA CTGGTATTAT TCTAACTTTA GCTATGAATA TTATGAGTAC TTTGCAATGG 
GCTGTGAACT CAAGCATTGA TACAGATAGC TTGATGCGAT CTGTGAGCAG AGTCTTTAAG 
TTTATTGATA TACAAACAGA AGAAAGTATA TGTACCAAGA TAATGAAAGA ACTACATAGT 
GAAGACTCAC CTAATGCTTT AGTCATTAAG AATGAACATG TGAAGAAATG TGATACCTGG 
CCCTCTGGAG GTGAAATGGT TGTCAAAGAC CTTACTGTGA AATATGTGGA TGATGGGAAT 
GCCATATTAG AGAACATTTC TTTTTCAATA AGTCCTGGCC AGAGGGTGGG GCTCTTAGGA 
AGAACTGGCT CAGGAAAAAG TACTTTGCTG TCAGCCTTTT TACGAATGTT GAACATTAAA 
GGCGAAATAC AGATCGATGG TGTCTCCTGG AACTCAATGA CCTTACAAGA ATGGAGAAAA 
GCTTTTGGAG TGATAACACA GAAAGTATTT ATCTTTTCTG GAACATTCAG ACAAAACTTG 
GATCCCAATG GAAAATGGAG AGATGAAGAA ATATGGAAAG TTGCAGATCA GGTTGGCCTC 
AAGTCTGTGA TAGAGCAGTT TCCTGGACAG CTCAACTTTA CCCTTGTGGA TGGGGGTTAT 
GTGCTAAGCC ATGGTCACAA GCAGTTGATG TGCTTGGCCC GATCTGTTCT CAGTAAGGCC 
AAGATCATAC TGCTTGATGA GCCTAGTGCC AATCTAGACC CCATAACATA CCAAGTGATT 
CGACGAGTTC TAAGACAAGC CTTCGCTGGT TGCACAGTAG TCCTCTGTGA ACACAGGATA 
GAAGCAATGT TGGACTGTCA GCGATTTTTG GTCATAGAGC AGGGCAATGT CTGGCAGTAT 
GAATCCCTCC AGGCACTTCT GAGCGAGAAG AGTGTCTTCC AGCGAGCCCT TAGCTCCTCA 
GAAAAGATGA AGCTCTTCCA CGGCCGGCAC TCCAGCAAGC AGAAGCCTCG GACACAGATC 
ACTGCTGTGA AAGAGGAGAC GGAGGAAGAA GTGCAAGAGA CCCGGCTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cftr ( XM_006236097.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006236159.1 Rattus norvegicus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa27035
Clone ID Related Accession (Same CDS sequence)	XM_006236097.3
Accession Version	XM_006236097.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cystic fibrosis transmembrane conductance regulator isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006236097.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTACC CGGGCCCGCT GTCTGAGCCA CTGCTGGGGC CACCATGTGA AGGACTGAGT 
AAAAGGGAAG AGCCAAAAGC ATTGAACGGG TCCTGGATAT CCAGAAGTCG AGTCCAACCT 
GAACCTGTCC GGACACAGAC CTTAGTGCAC CACGGAGACA TCATGCAGAA GTCGCCTCTG 
GAGAAAGCCA GCTTTATCTC CAAACTCTTC TTCAGCTGGA CCACACCAAT TTTGAGAAAA 
GGGTACAGAC ACCACTTGGA GCTGTCAGAC ATATACCAAG CCCCTTCTTC TGATTCAGCT 
GACCACCTGT CTGAAAAGCT AGAAAGAGAA TGGGACAGGG AACAAGCTTC CAAAAAGAAG 
CCCCAGCTTA TCCACGCCCT CCGGCGATGC TTTGTCTGGA GATTCGTCTT CTACGGAGTC 
TTGTTATACC TCGGGGAAGT CACCAAGGCT GTCCAGCCTG TCTTGCTAGG AAGAATCATA 
GCATCCTATG ATCCAGACAA CACGGAGGAG CGTTCCATTG CCATTTACCT AGGCATAGGC 
TTATGCCTCC TCTTCATTGT CAGGACACTG CTTCTGCACC CAGCTATTTT TGGCCTTCAT 
CACATTGGAA TGCAGATGAG AATAGCCATG TTTAGCTTGA TTTATAAGAA GACTTTAAAG 
TTGTCAAGCC GTGTTCTTGA TAAAATAAGT ATTGGACAAC TTATTAGTCT TCTTTCCAAC 
AACCTGAACA AATTTGATGA AGGACTTGCC TTGGCACATT TTATATGGAT TGCTCCTTTA 
CAAGTGGTTC TTCTGATGGG GCTTCTCTGG GACTTGTTAC AGTTCTCAGC CTTCTGTGGC 
CTTGGTTTAC TGATAGTCTT GGTTATTTTT CAAGCTATCT TAGGGAAGAT GATGGTGAAG 
TACAGAGATA AAAGAGCTGC AAAGATCAAT GAAAGACTTG TGATCACATC AGAAGTTATT 
GACAATATCT ATTCTGTTAA GGCATATTGT TGGGAATCAG CGATGGAGAA GATAATTGAA 
AGCTTGAGAG AGGAGGAACT GAAAATGACC CGGAGGTCAG CCTATATGAG GTTCTTCACT 
AGCTCTGCCT TCTTCTTTTC AGGGTTTTTT GTAGTCTTTT TATCTGTGCT TCCCTACACA 
GTCATCAATG GAATCGTCCT CCGAAAAATA TTCACAACCA TTTCATTCTG CATTGTCCTG 
CGTATGTCAG TCACACGGCA ATTCCCCACT GCTGTACAGA TATGGTATGA TTCTTTGGGA 
ATGATAAGAA AAATACAGGA TTTCCTACAG ACTCAAGAAT ATAAAGTGCT GGAGTATAAC 
TTAATGTTCA CAGGCCTAGT CATGGAGAAT GTAACAGCAT TTTGGGAGGA GGGATTTCAG 
GAATTACTGG AGAAAGTACA ACTAAACAAT GATGACAGAA AAACTTCCAA TGGTGAGAAC 
CACCTCAGTT TTAGTCATCT CTGTCTAGTG GGAAATCCTG TGTTGAAAAA CATCAATTTG 
AATATAAAGA AAGGAGAGAT GTTGGCTATT ACTGGATCTA CTGGAGCAGG AAAGACATCA 
CTCCTGATGC TGATACTGGG AGAACTGGAA GCGTCAGAGG GAATTATTAA GCACAGTGGA 
AGAGTTTCAT TCTCCTCTCA AATTTCTTGG ATTATGCCGG GTACTATCAA AGAAAATATC 
ATCTTTGGTG TTTCCTATGA TGAGTACAGA TATAAGAGTG TTGTCAAAGC TTGCCAACTA 
CAGGAGGACA TCACCAAGTT TGCAGAACAA GACAACACAG TTCTTGGAGA AGGTGGAGTC 
ACACTCAGTG GAGGTCAACG TGCAAGAATT TCTTTAGCAA GAGCAGTATA TAAAGATGCT 
GATTTGTACC TGTTAGATTC CCCCTTCGGA TATCTAGATG TTTTAACTGA AGAACAAATA 
TTTGAAAGTT GTGTCTGTAA ATTGATGGCC AGCAAAACTA GGATTCTGGT CACATCTAAA 
ATGGAGCAGT TAAAGAAAGC TGACAAGATA CTCATTTTGC ATGAGGGCAG CAGCTATTTT 
TATGGGACGT TTTCTGAACT ACAAAGTCTA CGTCCAGACT TCAGTTCCAA ACTCATGGGG 
TATGATACTT TTGACCAGTT TACTGAGGAA AGAAGGAGTT CAATTCTAAC TGAGACCTTA 
CGCAGGTTCT CAGTGGACGA TGCCTCTACC ACCTGGAACA AAGCCAAACA GTCATTTAGA 
CAGACTGGAG AGTTTGGAGA AAAAAGGAAG AACTCTATTC TAAGTTCATT CAGCTCTGTG 
AAGAAAATTT CCATTGTGCA AAAGACTCCA TTGTCCATAG AAGGAGAATC TGATGACCTC 
CAAGAAAGGA GACTGTCCCT GGTTCCAGAC TCTGAACATG GAGAGGCCGC TCTGCCTCGC 
AGCAACATGA TCACCGCCGG GCCCACGTTT CCAGGCAGAA GAAGACAGTC TGTTTTGGAT 
CTTATGACAT TCACACCCAG CTCAGTCTCC AGCAGTCTCC AGAGGACCAG AGCTTCTATT 
CGAAAAATAT CCTTAGCCCC TCGGATAAGC TTAAAGGAAG AGGATATATA TTCAAGGCGA 
TTATCGCAAG ATAGCACACT GAACATCACC GAAGAAATTA ATGAAGAAGA TTTAAAGGAG 
TGTTTTTTTG ATGATATGGT AAAGATCCCC ACGGTGACAA CATGGAACAC ATACCTTCGA 
TATTTCACGC TCCACAGAGG CTTATTTGCA GTGCTGATTT GGTGCGTGCT GGTTTTTCTG 
GTTGAGGTGG CTGCTTCTTT ATTTGTGTTG TGGTTGCTTA AAAACAACCC CGTTAATGGT 
GGAAACAATG GCACTAAAAT TGCCAATACC TCCTATGTTG TGGTCATCAC CAGTTCCAGC 
TTCTATTACA TTTTTTACAT TTACGTGGGA GTGGCTGACA CTTTGCTTGC TCTGAGCCTC 
TTCAGAGGTT TGCCCCTGGT GCATACGTTA ATCACAGCAT CAAAAATTTT GCACAGGAAA 
ATGTTACATT CTATTCTTCA TGCCCCTATG TCAACCTTCA ACAAGCTGAA AGCAGGTGGG 
ATTCTTAACA GATTCTCCAA AGATATAGCA ATTTTGGATG ACTTTCTGCC TCTTACCATA 
TTTGACTTCA TCCAGTTGCT GTTCATTGTG GTCGGAGCAA TAATAGTTGT CTCGGCTTTA 
CAACCCTACA TCTTCCTAGC AACGGTGCCA GGGCTAGCAG TCTTCATTTT ACTGAGGGCA 
TACTTCCTTC ATACCTCCCA ACAGCTCAAA CAACTGGAAT CTGAAGGCAG GAGTCCAATT 
TTCACGCACC TTGTTACAAG CTTAAAAGGA CTGTGGACAC TTCGAGCCTT CCGACGCCAG 
ACTTACTTTG AAACCCTATT CCACAAAGCT CTGAATTTGC ACACTGCCAA CTGGTTTATG 
TATCTGGCAA CCTTGCGCTG GTTCCAGATG AGAATAGACA TGATTTTCGT CCTCTTCTTC 
ATTGTTGTTA CCTTCATCTC CATTTTAACA ACAGGTGAAG GAGAAGGAAC GACTGGTATT 
ATTCTAACTT TAGCTATGAA TATTATGAGT ACTTTGCAAT GGGCTGTGAA CTCAAGCATT 
GATACAGATA GCTTGATGCG ATCTGTGAGC AGAGTCTTTA AGTTTATTGA TATACAAACA 
GAAGAAAGTA TATGTACCAA GATAATGAAA GAACTACATA GTGAAGACTC ACCTAATGCT 
TTAGTCATTA AGAATGAACA TGTGAAGAAA TGTGATACCT GGCCCTCTGG AGGTGAAATG 
GTTGTCAAAG ACCTTACTGT GAAATATGTG GATGATGGGA ATGCCATATT AGAGAACATT 
TCTTTTTCAA TAAGTCCTGG CCAGAGGGTG GGGCTCTTAG GAAGAACTGG CTCAGGAAAA 
AGTACTTTGC TGTCAGCCTT TTTACGAATG TTGAACATTA AAGGCGAAAT ACAGATCGAT 
GGTGTCTCCT GGAACTCAAT GACCTTACAA GAATGGAGAA AAGCTTTTGG AGTGATAACA 
CAGAAAGTAT TTATCTTTTC TGGAACATTC AGACAAAACT TGGATCCCAA TGGAAAATGG 
AGAGATGAAG AAATATGGAA AGTTGCAGAT CAGGTTGGCC TCAAGTCTGT GATAGAGCAG 
TTTCCTGGAC AGCTCAACTT TACCCTTGTG GATGGGGGTT ATGTGCTAAG CCATGGTCAC 
AAGCAGTTGA TGTGCTTGGC CCGATCTGTT CTCAGTAAGG CCAAGATCAT ACTGCTTGAT 
GAGCCTAGTG CCAATCTAGA CCCCATAACA TACCAAGTGA TTCGACGAGT TCTAAGACAA 
GCCTTCGCTG GTTGCACAGT AGTCCTCTGT GAACACAGGA TAGAAGCAAT GTTGGACTGT 
CAGCGATTTT TGGTCATAGA GCAGGGCAAT GTCTGGCAGT ATGAATCCCT CCAGGCACTT 
CTGAGCGAGA AGAGTGTCTT CCAGCGAGCC CTTAGCTCCT CAGAAAAGAT GAAGCTCTTC 
CACGGCCGGC ACTCCAGCAA GCAGAAGCCT CGGACACAGA TCACTGCTGT GAAAGAGGAG 
ACGGAGGAAG AAGTGCAAGA GACCCGGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006236159.1
CDS	106..4698
Translation	MVYPGPLSEPLLGPPCEGLSKREEPKALNGSWISRSRVQPEPVRTQTLVHHGDIMQKSPLEKASFISKLFFSWTTPILRKGYRHHLELSDIYQAPSSDSADHLSEKLEREWDREQASKKKPQLIHALRRCFVWRFVFYGVLLYLGEVTKAVQPVLLGRIIASYDPDNTEERSIAIYLGIGLCLLFIVRTLLLHPAIFGLHHIGMQMRIAMFSLIYKKTLKLSSRVLDKISIGQLISLLSNNLNKFDEGLALAHFIWIAPLQVVLLMGLLWDLLQFSAFCGLGLLIVLVIFQAILGKMMVKYRDKRAAKINERLVITSEVIDNIYSVKAYCWESAMEKIIESLREEELKMTRRSAYMRFFTSSAFFFSGFFVVFLSVLPYTVINGIVLRKIFTTISFCIVLRMSVTRQFPTAVQIWYDSLGMIRKIQDFLQTQEYKVLEYNLMFTGLVMENVTAFWEEGFQELLEKVQLNNDDRKTSNGENHLSFSHLCLVGNPVLKNINLNIKKGEMLAITGSTGAGKTSLLMLILGELEASEGIIKHSGRVSFSSQISWIMPGTIKENIIFGVSYDEYRYKSVVKACQLQEDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEEQIFESCVCKLMASKTRILVTSKMEQLKKADKILILHEGSSYFYGTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDASTTWNKAKQSFRQTGEFGEKRKNSILSSFSSVKKISIVQKTPLSIEGESDDLQERRLSLVPDSEHGEAALPRSNMITAGPTFPGRRRQSVLDLMTFTPSSVSSSLQRTRASIRKISLAPRISLKEEDIYSRRLSQDSTLNITEEINEEDLKECFFDDMVKIPTVTTWNTYLRYFTLHRGLFAVLIWCVLVFLVEVAASLFVLWLLKNNPVNGGNNGTKIANTSYVVVITSSSFYYIFYIYVGVADTLLALSLFRGLPLVHTLITASKILHRKMLHSILHAPMSTFNKLKAGGILNRFSKDIAILDDFLPLTIFDFIQLLFIVVGAIIVVSALQPYIFLATVPGLAVFILLRAYFLHTSQQLKQLESEGRSPIFTHLVTSLKGLWTLRAFRRQTYFETLFHKALNLHTANWFMYLATLRWFQMRIDMIFVLFFIVVTFISILTTGEGEGTTGIILTLAMNIMSTLQWAVNSSIDTDSLMRSVSRVFKFIDIQTEESICTKIMKELHSEDSPNALVIKNEHVKKCDTWPSGGEMVVKDLTVKYVDDGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGEIQIDGVSWNSMTLQEWRKAFGVITQKVFIFSGTFRQNLDPNGKWRDEEIWKVADQVGLKSVIEQFPGQLNFTLVDGGYVLSHGHKQLMCLARSVLSKAKIILLDEPSANLDPITYQVIRRVLRQAFAGCTVVLCEHRIEAMLDCQRFLVIEQGNVWQYESLQALLSEKSVFQRALSSSEKMKLFHGRHSSKQKPRTQITAVKEETEEEVQETRL

Target ORF information:

RefSeq Version	XM_006236097.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cystic fibrosis transmembrane conductance regulator (Cftr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006236097.3

ORF Insert Sequence:

ATGGTTTACC CGGGCCCGCT GTCTGAGCCA CTGCTGGGGC CACCATGTGA AGGACTGAGT 
AAAAGGGAAG AGCCAAAAGC ATTGAACGGG TCCTGGATAT CCAGAAGTCG AGTCCAACCT 
GAACCTGTCC GGACACAGAC CTTAGTGCAC CACGGAGACA TCATGCAGAA GTCGCCTCTG 
GAGAAAGCCA GCTTTATCTC CAAACTCTTC TTCAGCTGGA CCACACCAAT TTTGAGAAAA 
GGGTACAGAC ACCACTTGGA GCTGTCAGAC ATATACCAAG CCCCTTCTTC TGATTCAGCT 
GACCACCTGT CTGAAAAGCT AGAAAGAGAA TGGGACAGGG AACAAGCTTC CAAAAAGAAG 
CCCCAGCTTA TCCACGCCCT CCGGCGATGC TTTGTCTGGA GATTCGTCTT CTACGGAGTC 
TTGTTATACC TCGGGGAAGT CACCAAGGCT GTCCAGCCTG TCTTGCTAGG AAGAATCATA 
GCATCCTATG ATCCAGACAA CACGGAGGAG CGTTCCATTG CCATTTACCT AGGCATAGGC 
TTATGCCTCC TCTTCATTGT CAGGACACTG CTTCTGCACC CAGCTATTTT TGGCCTTCAT 
CACATTGGAA TGCAGATGAG AATAGCCATG TTTAGCTTGA TTTATAAGAA GACTTTAAAG 
TTGTCAAGCC GTGTTCTTGA TAAAATAAGT ATTGGACAAC TTATTAGTCT TCTTTCCAAC 
AACCTGAACA AATTTGATGA AGGACTTGCC TTGGCACATT TTATATGGAT TGCTCCTTTA 
CAAGTGGTTC TTCTGATGGG GCTTCTCTGG GACTTGTTAC AGTTCTCAGC CTTCTGTGGC 
CTTGGTTTAC TGATAGTCTT GGTTATTTTT CAAGCTATCT TAGGGAAGAT GATGGTGAAG 
TACAGAGATA AAAGAGCTGC AAAGATCAAT GAAAGACTTG TGATCACATC AGAAGTTATT 
GACAATATCT ATTCTGTTAA GGCATATTGT TGGGAATCAG CGATGGAGAA GATAATTGAA 
AGCTTGAGAG AGGAGGAACT GAAAATGACC CGGAGGTCAG CCTATATGAG GTTCTTCACT 
AGCTCTGCCT TCTTCTTTTC AGGGTTTTTT GTAGTCTTTT TATCTGTGCT TCCCTACACA 
GTCATCAATG GAATCGTCCT CCGAAAAATA TTCACAACCA TTTCATTCTG CATTGTCCTG 
CGTATGTCAG TCACACGGCA ATTCCCCACT GCTGTACAGA TATGGTATGA TTCTTTGGGA 
ATGATAAGAA AAATACAGGA TTTCCTACAG ACTCAAGAAT ATAAAGTGCT GGAGTATAAC 
TTAATGTTCA CAGGCCTAGT CATGGAGAAT GTAACAGCAT TTTGGGAGGA GGGATTTCAG 
GAATTACTGG AGAAAGTACA ACTAAACAAT GATGACAGAA AAACTTCCAA TGGTGAGAAC 
CACCTCAGTT TTAGTCATCT CTGTCTAGTG GGAAATCCTG TGTTGAAAAA CATCAATTTG 
AATATAAAGA AAGGAGAGAT GTTGGCTATT ACTGGATCTA CTGGAGCAGG AAAGACATCA 
CTCCTGATGC TGATACTGGG AGAACTGGAA GCGTCAGAGG GAATTATTAA GCACAGTGGA 
AGAGTTTCAT TCTCCTCTCA AATTTCTTGG ATTATGCCGG GTACTATCAA AGAAAATATC 
ATCTTTGGTG TTTCCTATGA TGAGTACAGA TATAAGAGTG TTGTCAAAGC TTGCCAACTA 
CAGGAGGACA TCACCAAGTT TGCAGAACAA GACAACACAG TTCTTGGAGA AGGTGGAGTC 
ACACTCAGTG GAGGTCAACG TGCAAGAATT TCTTTAGCAA GAGCAGTATA TAAAGATGCT 
GATTTGTACC TGTTAGATTC CCCCTTCGGA TATCTAGATG TTTTAACTGA AGAACAAATA 
TTTGAAAGTT GTGTCTGTAA ATTGATGGCC AGCAAAACTA GGATTCTGGT CACATCTAAA 
ATGGAGCAGT TAAAGAAAGC TGACAAGATA CTCATTTTGC ATGAGGGCAG CAGCTATTTT 
TATGGGACGT TTTCTGAACT ACAAAGTCTA CGTCCAGACT TCAGTTCCAA ACTCATGGGG 
TATGATACTT TTGACCAGTT TACTGAGGAA AGAAGGAGTT CAATTCTAAC TGAGACCTTA 
CGCAGGTTCT CAGTGGACGA TGCCTCTACC ACCTGGAACA AAGCCAAACA GTCATTTAGA 
CAGACTGGAG AGTTTGGAGA AAAAAGGAAG AACTCTATTC TAAGTTCATT CAGCTCTGTG 
AAGAAAATTT CCATTGTGCA AAAGACTCCA TTGTCCATAG AAGGAGAATC TGATGACCTC 
CAAGAAAGGA GACTGTCCCT GGTTCCAGAC TCTGAACATG GAGAGGCCGC TCTGCCTCGC 
AGCAACATGA TCACCGCCGG GCCCACGTTT CCAGGCAGAA GAAGACAGTC TGTTTTGGAT 
CTTATGACAT TCACACCCAG CTCAGTCTCC AGCAGTCTCC AGAGGACCAG AGCTTCTATT 
CGAAAAATAT CCTTAGCCCC TCGGATAAGC TTAAAGGAAG AGGATATATA TTCAAGGCGA 
TTATCGCAAG ATAGCACACT GAACATCACC GAAGAAATTA ATGAAGAAGA TTTAAAGGAG 
TGTTTTTTTG ATGATATGGT AAAGATCCCC ACGGTGACAA CATGGAACAC ATACCTTCGA 
TATTTCACGC TCCACAGAGG CTTATTTGCA GTGCTGATTT GGTGCGTGCT GGTTTTTCTG 
GTTGAGGTGG CTGCTTCTTT ATTTGTGTTG TGGTTGCTTA AAAACAACCC CGTTAATGGT 
GGAAACAATG GCACTAAAAT TGCCAATACC TCCTATGTTG TGGTCATCAC CAGTTCCAGC 
TTCTATTACA TTTTTTACAT TTACGTGGGA GTGGCTGACA CTTTGCTTGC TCTGAGCCTC 
TTCAGAGGTT TGCCCCTGGT GCATACGTTA ATCACAGCAT CAAAAATTTT GCACAGGAAA 
ATGTTACATT CTATTCTTCA TGCCCCTATG TCAACCTTCA ACAAGCTGAA AGCAGGTGGG 
ATTCTTAACA GATTCTCCAA AGATATAGCA ATTTTGGATG ACTTTCTGCC TCTTACCATA 
TTTGACTTCA TCCAGTTGCT GTTCATTGTG GTCGGAGCAA TAATAGTTGT CTCGGCTTTA 
CAACCCTACA TCTTCCTAGC AACGGTGCCA GGGCTAGCAG TCTTCATTTT ACTGAGGGCA 
TACTTCCTTC ATACCTCCCA ACAGCTCAAA CAACTGGAAT CTGAAGGCAG GAGTCCAATT 
TTCACGCACC TTGTTACAAG CTTAAAAGGA CTGTGGACAC TTCGAGCCTT CCGACGCCAG 
ACTTACTTTG AAACCCTATT CCACAAAGCT CTGAATTTGC ACACTGCCAA CTGGTTTATG 
TATCTGGCAA CCTTGCGCTG GTTCCAGATG AGAATAGACA TGATTTTCGT CCTCTTCTTC 
ATTGTTGTTA CCTTCATCTC CATTTTAACA ACAGGTGAAG GAGAAGGAAC GACTGGTATT 
ATTCTAACTT TAGCTATGAA TATTATGAGT ACTTTGCAAT GGGCTGTGAA CTCAAGCATT 
GATACAGATA GCTTGATGCG ATCTGTGAGC AGAGTCTTTA AGTTTATTGA TATACAAACA 
GAAGAAAGTA TATGTACCAA GATAATGAAA GAACTACATA GTGAAGACTC ACCTAATGCT 
TTAGTCATTA AGAATGAACA TGTGAAGAAA TGTGATACCT GGCCCTCTGG AGGTGAAATG 
GTTGTCAAAG ACCTTACTGT GAAATATGTG GATGATGGGA ATGCCATATT AGAGAACATT 
TCTTTTTCAA TAAGTCCTGG CCAGAGGGTG GGGCTCTTAG GAAGAACTGG CTCAGGAAAA 
AGTACTTTGC TGTCAGCCTT TTTACGAATG TTGAACATTA AAGGCGAAAT ACAGATCGAT 
GGTGTCTCCT GGAACTCAAT GACCTTACAA GAATGGAGAA AAGCTTTTGG AGTGATAACA 
CAGAAAGTAT TTATCTTTTC TGGAACATTC AGACAAAACT TGGATCCCAA TGGAAAATGG 
AGAGATGAAG AAATATGGAA AGTTGCAGAT CAGGTTGGCC TCAAGTCTGT GATAGAGCAG 
TTTCCTGGAC AGCTCAACTT TACCCTTGTG GATGGGGGTT ATGTGCTAAG CCATGGTCAC 
AAGCAGTTGA TGTGCTTGGC CCGATCTGTT CTCAGTAAGG CCAAGATCAT ACTGCTTGAT 
GAGCCTAGTG CCAATCTAGA CCCCATAACA TACCAAGTGA TTCGACGAGT TCTAAGACAA 
GCCTTCGCTG GTTGCACAGT AGTCCTCTGT GAACACAGGA TAGAAGCAAT GTTGGACTGT 
CAGCGATTTT TGGTCATAGA GCAGGGCAAT GTCTGGCAGT ATGAATCCCT CCAGGCACTT 
CTGAGCGAGA AGAGTGTCTT CCAGCGAGCC CTTAGCTCCT CAGAAAAGAT GAAGCTCTTC 
CACGGCCGGC ACTCCAGCAA GCAGAAGCCT CGGACACAGA TCACTGCTGT GAAAGAGGAG 
ACGGAGGAAG AAGTGCAAGA GACCCGGCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reduced bone length, growth plate thickness, bone content, and IGF-I as a model for poor growth in the CFTR-deficient rat.
PloS one12(11)e0188497(2017)
Stalvey MS,Havasi V,Tuggle KL,Wang D,Birket S,Rowe SM,Sorscher EJ

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Cftr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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