Add2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Add2
Entrez Gene ID	24171
Full Name	adducin 2
Synonyms	beta-ADD
General protein information	Preferred Names adducin 2 Names beta-adducin adducin 2 (beta) adducin beta adducin-63 erythrocyte adducin subunit beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34
Summary	plays a role in membrane ion transport; variation is associated with blood pressure variation in the Milan hypertensive strain [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592433.1	XP_017447922.1	beta-adducin isoform X2
NM_001109880.1	NP_001103350.1	beta-adducin isoform a
NM_012491.2	NP_036623.1	beta-adducin isoform b
XM_017592432.1	XP_017447921.1	beta-adducin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ADD2	NP_001171983.1
Mus musculus (house mouse)	Add2	NP_001258786.1
	ADD2	XP_002799318.1
Danio rerio (zebrafish)	add2	XP_698680.6
Pan troglodytes (chimpanzee)	ADD2	XP_003309148.1
Canis lupus familiaris (dog)	ADD2	XP_853066.1
Xenopus tropicalis (tropical clawed frog)	add2	NP_001096433.1
Bos taurus (cattle)	ADD2	NP_001092479.1
Rattus norvegicus (Norway rat)	Add2	NP_001103350.1

Gene Ontology
Bibliography

Related articles in PubMed

DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons.
Engmann O, Giralt A, Gervasi N, Marion-Poll L, Gasmi L, Filhol O, Picciotto MR, Gilligan D, Greengard P, Nairn AC, Herv? D, Girault JA
Nature communications610099(2015 Dec)

Lipid raft-associated ?-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin.
Xu T, Liu W, Yang C, Ba X, Wang X, Jiang Y, Zeng X
Journal of leukocyte biology97(2)297-306(2015 Feb)

[Association and interaction of AGT, AGTR1, ACE, ADRB2, DRD1, ADD1, ADD2, ATP2B1, TBXA2R and PTGS2 genes on the risk of hypertension in Antioquian population].
Valencia DM, Naranjo CA, Parra MV, Caro MA, Valencia AV, Jaramillo CJ, Bedoya G
Biomedica : revista del Instituto Nacional de Salud33(4)598-614(2013 Oct-Dec)

Mutation in the beta adducin subunit causes tissue-specific damage to myogenic tone.
Sonoyama K, Greenstein AS, Micheletti R, Ferrari P, Schiavone A, Aghamohammadzadeh R, Withers SB, Tripodi G, Ferrandi M, Heagerty AM
Journal of hypertension29(3)466-74(2011 Mar)

?-Adducin is required for stable assembly of new synapses and improved memory upon environmental enrichment.
Bednarek E, Caroni P
Neuron69(6)1132-46(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Darpp32 interaction with beta-adducin may be the molecular pathway by which environmental changes may rapidly alter responsiveness of striatal neurons involved in the reward system.
Title: DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons.

Mutations in the beta subunit of the adducin protein result in damage to renal artery myogenic tone and this is associated with renal damage as manifest by proteinuria.
Title: Mutation in the beta adducin subunit causes tissue-specific damage to myogenic tone.

Data show that the pY489-beta-adducin immunoreactivity is located in the hypothalamic tanycytes and is enhanced after fasting for 36-48 h.
Title: Localization of phospho-tyrosine489-beta-adducin immunoreactivity in the hypothalamic tanycytes and its involvement in energy homeostasis.

Expression of the hypertensive rat or human variant of adducin into normal renal epithelial cells recreates the hypertensive phenotype with higher Na+,K+-ATPase activity, mu2-subunit hyperphosphorylation, and impaired Na+,K+-ATPase endocytosis.
Title: Hypertension-linked mutation in the adducin alpha-subunit leads to higher AP2-mu2 phosphorylation and impaired Na+,K+-ATPase trafficking in response to GPCR signals and intracellular sodium.

beta-adducin plays a role in hypertension
Title: Tissue-specific modulation of beta-adducin transcripts in Milan hypertensive rats.

The following Add2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Add2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa42643	NM_012491.2 Latest version!	Rattus norvegicus adducin 2 (Add2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$328.30 $469.00
ORa42637	NM_001109880.1 Latest version!	Rattus norvegicus adducin 2 (Add2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ORa42637	XM_017592432.1 Latest version!	Rattus norvegicus adducin 2 (Add2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ORa60722	XM_017592433.1 Latest version!	Rattus norvegicus adducin 2 (Add2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Add2 ( NM_012491.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036623.1 Rattus norvegicus adducin 2 (Add2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa42643
Clone ID Related Accession (Same CDS sequence)	NM_012491.2
Accession Version	NM_012491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1689bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	beta-adducin isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M63894.1, EV762188.1 and BE121149.1. On Oct 10, 2007 this sequence version replaced NM_012491.1. Transcript Variant: This variant (2) contains a distinct 3' UTR and lacks a portion of the 3' coding region, compared to variant 1. The resulting isoform (b) has a shorter, unique C-terminus when compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: M63894.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGGTACAGC AGAGACTGCC CCCAACTGAA 
GGGGAAGCTT ATCAGACTCC TGGGGCTGGG CAGGGGACCC CTGAGTCCTC AGGCCCACTC 
ACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036623.1
CDS	188..1876
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPVQQRLPPTEGEAYQTPGAGQGTPESSGPLTP

Target ORF information:

RefSeq Version	NM_012491.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adducin 2 (Add2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012491.2

ORF Insert Sequence:

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGGTACAGC AGAGACTGCC CCCAACTGAA 
GGGGAAGCTT ATCAGACTCC TGGGGCTGGG CAGGGGACCC CTGAGTCCTC AGGCCCACTC 
ACCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( NM_001109880.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001103350.1 Rattus norvegicus adducin 2 (Add2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa42637
Clone ID Related Accession (Same CDS sequence)	NM_001109880.1 , XM_017592432.1
Accession Version	NM_001109880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-adducin isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M63894.1, AF130338.1 and DY315967.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GGCGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATTGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAAGAAGGGG AGAAGGACGC AGCCACAGAG GAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCGTCTCCTG TGCAGAGCCC AACGAGGGCA GGGACCAAGA GTCCAGCGGT CTCTCCTTCC 
AAGGCTTCAG AGGATGCCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GAGAAACCAG AAGGGGTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGTGT GGAAGAGGTC 
CTCAGCAAAG GACCGGGCCA GATGACTACC AATGCTGACA CTGATGGCGA CAGTTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCCTTCC TGAAGAAGAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103350.1
CDS	188..2365
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPSTESQLASKGDADTKDELEETVPNPFSQLTDQELEEYKKEVERKKLEQEQEGEKDAATEEPGSPVKSTPASPVQSPTRAGTKSPAVSPSKASEDAKKTEVSEANTEPEPEKPEGVVVNGKEEEPSVEEVLSKGPGQMTTNADTDGDSYKDKTESVTSGPLSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Target ORF information:

RefSeq Version	NM_001109880.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adducin 2 (Add2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109880.1

ORF Insert Sequence:

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GGCGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATTGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAAGAAGGGG AGAAGGACGC AGCCACAGAG GAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCGTCTCCTG TGCAGAGCCC AACGAGGGCA GGGACCAAGA GTCCAGCGGT CTCTCCTTCC 
AAGGCTTCAG AGGATGCCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GAGAAACCAG AAGGGGTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGTGT GGAAGAGGTC 
CTCAGCAAAG GACCGGGCCA GATGACTACC AATGCTGACA CTGATGGCGA CAGTTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCCTTCC TGAAGAAGAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( XM_017592432.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447921.1 Rattus norvegicus adducin 2 (Add2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa42637
Clone ID Related Accession (Same CDS sequence)	NM_001109880.1 , XM_017592432.1
Accession Version	XM_017592432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-adducin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GGCGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATTGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAAGAAGGGG AGAAGGACGC AGCCACAGAG GAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCGTCTCCTG TGCAGAGCCC AACGAGGGCA GGGACCAAGA GTCCAGCGGT CTCTCCTTCC 
AAGGCTTCAG AGGATGCCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GAGAAACCAG AAGGGGTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGTGT GGAAGAGGTC 
CTCAGCAAAG GACCGGGCCA GATGACTACC AATGCTGACA CTGATGGCGA CAGTTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCCTTCC TGAAGAAGAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447921.1
CDS	409..2586
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPSTESQLASKGDADTKDELEETVPNPFSQLTDQELEEYKKEVERKKLEQEQEGEKDAATEEPGSPVKSTPASPVQSPTRAGTKSPAVSPSKASEDAKKTEVSEANTEPEPEKPEGVVVNGKEEEPSVEEVLSKGPGQMTTNADTDGDSYKDKTESVTSGPLSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

Target ORF information:

RefSeq Version	XM_017592432.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adducin 2 (Add2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592432.1

ORF Insert Sequence:

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GGCGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATTGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAAGAAGGGG AGAAGGACGC AGCCACAGAG GAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCGTCTCCTG TGCAGAGCCC AACGAGGGCA GGGACCAAGA GTCCAGCGGT CTCTCCTTCC 
AAGGCTTCAG AGGATGCCAA GAAGACAGAA GTCAGCGAAG CCAACACAGA GCCTGAGCCA 
GAGAAACCAG AAGGGGTGGT GGTGAATGGG AAGGAGGAGG AGCCGAGTGT GGAAGAGGTC 
CTCAGCAAAG GACCGGGCCA GATGACTACC AATGCTGACA CTGATGGCGA CAGTTACAAG 
GACAAAACCG AGTCAGTTAC CAGTGGCCCC TTGTCCCCAG AGGGCTCGCC CTCTAAGTCA 
CCCTCCAAAA AGAAAAAGAA ATTCCGAACC CCCTCCTTCC TGAAGAAGAG CAAGAAGAAG 
GAGAAGGTGG AATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Add2 ( XM_017592433.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447922.1 Rattus norvegicus adducin 2 (Add2), transcript variant X2, mRNA.

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CloneID	ORa60722
Clone ID Related Accession (Same CDS sequence)	XM_017592433.1
Accession Version	XM_017592433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	beta-adducin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GGCGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATTGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAAGAAGGGG AGAAGGACGC AGCCACAGAG GAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCGTCTCCTG TGCAGAGCCC AACGAGGGCA GGGACCAAGA GTCCAGCGGT CTCTCCTTCC 
AAGGCTTCAG AGGGGTTTTC TTCCGAGTCT GAATACTTGT CTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447922.1
CDS	410..2317
Translation	MSEDTVPEAASPPPSQGQHYFDRFSEDDPEYLRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPASSMNFSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFSLHSAIYAARPDVRCAIHLHTPATAAVSAMKCGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGMVALGDTVEEAFYKVFHLQAACEVQVSALSSAGGTENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGVPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRNMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLDMRNKIREQNRQDIKSAGPQSQLLASVIAEKSRSPSTESQLASKGDADTKDELEETVPNPFSQLTDQELEEYKKEVERKKLEQEQEGEKDAATEEPGSPVKSTPASPVQSPTRAGTKSPAVSPSKASEGFSSESEYLSL

Target ORF information:

RefSeq Version	XM_017592433.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adducin 2 (Add2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592433.1

ORF Insert Sequence:

ATGAGTGAGG ACACGGTCCC CGAGGCAGCC TCACCGCCAC CCTCTCAGGG GCAGCACTAC 
TTTGACCGGT TCTCTGAGGA TGACCCTGAA TACTTGCGAC TTCGCAACCG TGCAGCTGAC 
CTGCGACAAG ACTTCAACCT GATGGAGCAG AAGAAACGGG TCACCATGAT CCTGCAGAGC 
CCTTCTTTCA GGGAGGAGCT GGAAGGCCTC ATCCAGGAAC AGATGAAGAA GGGTAACAAC 
TCCTCCAACA TCTGGGCCCT CCGACAGATC GCGGACTTCA TGGCCAGCAC CTCCCACGCA 
GTCTTCCCAG CTTCCTCCAT GAACTTCTCC ATGATGACAC CCATCAATGA CCTCCACACT 
GCCGACTCCC TGAACCTGGC CAAGGGGGAG AGGCTTATGC GGTGCAAGAT CAGCAGTGTC 
TACCGTCTTC TGGACCTCTA TGGCTGGGCG CAGCTCAGCG ACACCTATGT CACGCTGAGA 
GTGAGCAAGG AGCAGGACCA CTTCCTGATC AGCCCCAAAG GGGTTTCCTG CAGTGAGGTC 
ACGGCATCCA GCCTGATTAA GGTGAACATT CTAGGAGAGG TCGTGGAGAA GGGCAGCAGT 
TGCTTCCCAG TGGACACCAC CGGCTTCAGT CTGCACTCAG CCATCTATGC GGCCAGGCCG 
GACGTGCGGT GTGCCATCCA CCTGCACACG CCTGCCACAG CAGCGGTGTC AGCTATGAAG 
TGTGGCCTCC TGCCTGTCTC CCATAATGCC CTGCTGGTGG GGGACATGGC CTACTATGAC 
TTCAATGGGG AAATGGAGCA GGAAGCTGAT CGAATCAACT TGCAGAAGTG CCTTGGACCC 
ACCTGCAAGA TTCTGGTGCT AAGAAACCAT GGTATGGTCG CCCTGGGTGA CACCGTGGAG 
GAAGCTTTCT ACAAGGTCTT CCACCTGCAG GCTGCGTGTG AGGTACAGGT GTCGGCTCTG 
TCCAGCGCTG GGGGGACCGA GAACCTCATC CTCCTGGAGC AAGAGAAACA CCGTCCGCAT 
GAGGTGGGCT CTGTGCAGTG GGCCGGCAGC ACCTTTGGGC CCATGCAGAA GAGCCGGCTG 
GGAGAGCATG AATTTGAAGC CCTCATGAGG ATGCTCGACA ACTTAGGCTA CAGAACAGGC 
TATACGTACC GCCACCCCTT CGTCCAAGAG AAAACCAAAC ACAAAAGTGA AGTGGAGATC 
CCAGCCACGG TCACTGCCTT TGTGTTCGAA GAGGATGGTG TCCCAGTCCC TGCTCTGCGC 
CAGCACGCCC AAAAGCAGCA GAAGGAAAAG ACCCGCTGGC TTAACACTCC CAACACCTAC 
CTGAGGGTGA ATGTGGCCGA CGAGGTGCAG AGGAACATGG GCAGTCCCCG ACCGAAGACC 
ACGTGGATGA AGGCTGATGA AGTGGAAAAG TCCAGCAGCG GCATGCCCAT ACGGATTGAA 
AACCCAAACC AATTTGTGCC TCTCTACACT GACCCCCAAG AAGTGCTGGA CATGAGGAAC 
AAGATTCGAG AACAAAACCG ACAAGATATA AAGTCAGCCG GGCCTCAGTC TCAGCTCTTG 
GCCAGCGTCA TCGCCGAGAA GAGCCGGAGT CCGTCTACAG AGAGCCAGCT GGCGTCCAAG 
GGCGATGCAG ATACCAAAGA TGAATTGGAG GAGACGGTGC CCAACCCCTT CAGCCAACTC 
ACTGACCAGG AGCTGGAGGA GTACAAGAAG GAGGTGGAAA GGAAGAAGCT AGAACAGGAG 
CAAGAAGGGG AGAAGGACGC AGCCACAGAG GAGCCTGGTT CACCTGTAAA GTCCACACCT 
GCGTCTCCTG TGCAGAGCCC AACGAGGGCA GGGACCAAGA GTCCAGCGGT CTCTCCTTCC 
AAGGCTTCAG AGGGGTTTTC TTCCGAGTCT GAATACTTGT CTCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons.
Nature communications610099(2015 Dec)
Engmann O,Giralt A,Gervasi N,Marion-Poll L,Gasmi L,Filhol O,Picciotto MR,Gilligan D,Greengard P,Nairn AC,Herv? D,Girault JA

Lipid raft-associated ?-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin.
Journal of leukocyte biology97(2)297-306(2015 Feb)
Xu T,Liu W,Yang C,Ba X,Wang X,Jiang Y,Zeng X

[Association and interaction of AGT, AGTR1, ACE, ADRB2, DRD1, ADD1, ADD2, ATP2B1, TBXA2R and PTGS2 genes on the risk of hypertension in Antioquian population].
Biomedica : revista del Instituto Nacional de Salud33(4)598-614(2013 Oct-Dec)
Valencia DM,Naranjo CA,Parra MV,Caro MA,Valencia AV,Jaramillo CJ,Bedoya G

Mutation in the beta adducin subunit causes tissue-specific damage to myogenic tone.
Journal of hypertension29(3)466-74(2011 Mar)
Sonoyama K,Greenstein AS,Micheletti R,Ferrari P,Schiavone A,Aghamohammadzadeh R,Withers SB,Tripodi G,Ferrandi M,Heagerty AM

?-Adducin is required for stable assembly of new synapses and improved memory upon environmental enrichment.
Neuron69(6)1132-46(2011 Mar)
Bednarek E,Caroni P

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Add2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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